NOS3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOS3
Entrez Gene ID	287024
Full Name	nitric oxide synthase 3
General protein information	Preferred Names nitric oxide synthase 3 Names nitric oxide synthase, endothelial EC-NOS NOS type III NOSIII cNOS constitutive NOS eNOS endothelial NOS endothelial nitric oxide synthase nitric oxide synthase 3 (endothelial cell)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015470752.1	XP_015326238.1	nitric oxide synthase, endothelial isoform X1
XM_024990490.1	XP_024846258.1	nitric oxide synthase, endothelial isoform X1
NM_181037.3	NP_851380.2	nitric oxide synthase, endothelial

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NOS3	XP_003318952.1
	NOS3	XP_002803570.1
Xenopus tropicalis (tropical clawed frog)	nos3	NP_001243158.1
Bos taurus (cattle)	NOS3	NP_851380.2
Homo sapiens (human)	NOS3	NP_000594.2
Mus musculus (house mouse)	Nos3	NP_032739.3
Canis lupus familiaris (dog)	NOS3	NP_001003158.2
Rattus norvegicus (Norway rat)	Nos3	NP_068610.1

Gene Ontology
Bibliography

Related articles in PubMed

Activation of transient receptor potential vanilloid 1 decreases endothelial nitric oxide synthase phosphorylation at Thr497 by protein phosphatase 2B-dependent dephosphorylation of protein kinase C.
Ching LC, Zhao JF, Su KH, Shyue SK, Hsu CP, Lu TM, Lin SJ, Lee TS
Acta physiologica (Oxford, England)209(2)124-35(2013 Oct)

Calmodulin-induced structural changes in endothelial nitric oxide synthase.
Persechini A, Tran QK, Black DJ, Gogol EP
FEBS letters587(3)297-301(2013 Jan)

Placental expression and localization of endothelin-1 system and nitric oxide synthases during bovine pregnancy.
Hayashi KG, Hosoe M, Takahashi T
Animal reproduction science134(3-4)150-7(2012 Oct)

Protein kinase C? phosphorylates a novel argininosuccinate synthase site at serine 328 during calcium-dependent stimulation of endothelial nitric-oxide synthase in vascular endothelial cells.
Haines RJ, Corbin KD, Pendleton LC, Eichler DC
The Journal of biological chemistry287(31)26168-76(2012 Jul)

Proteomic identification of S-nitrosylated Golgi proteins: new insights into endothelial cell regulation by eNOS-derived NO.
Sangwung P, Greco TM, Wang Y, Ischiropoulos H, Sessa WC, Iwakiri Y
PloS one7(2)e31564(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Endothelial cell autophagy maintains shear stress-induced nitric oxide generation via glycolysis-dependent ATP/P2Y1 receptor signaling to endothelial nitric oxide synthase.
Title: Endothelial Cell Autophagy Maintains Shear Stress-Induced Nitric Oxide Generation via Glycolysis-Dependent Purinergic Signaling to Endothelial Nitric Oxide Synthase.

These data define the mechanism responsible for the repressive effects of nitric oxide (NO) on the transcriptional activity of beta-catenin and link eNOS-derived NO to the modulation by VEGF of Wnt/beta-catenin-induced endothelial cell proliferation.
Title: Endothelial NO Synthase-Dependent S-Nitrosylation of ?-Catenin Prevents Its Association with TCF4 and Inhibits Proliferation of Endothelial Cells Stimulated by Wnt3a.

S1179D substitution in eNOS increased the rate of flavin reduction, altered the conformational equilibrium of the reductase domain, and increased the rate of its conformational transitions.
Title: Phosphorylation Controls Endothelial Nitric-oxide Synthase by Regulating Its Conformational Dynamics.

TNFalpha reduces eNOS activity in bovine endothelial cells through serine 116 phosphorylation and Pin1 binding.
Title: TNF? reduces eNOS activity in endothelial cells through serine 116 phosphorylation and Pin1 binding: Confirmation of a direct, inhibitory interaction of Pin1 with eNOS.

Signals that activate and phosphorylate eNOS are transmitted through distinct membrane domains in endothelial cells. Cholesterol domains, but not individual caveolae, mediate HDL stimulation of eNOS. VEGF and shear stress may act through caveolae.
Title: Activation of Endothelial Nitric Oxide (eNOS) Occurs through Different Membrane Domains in Endothelial Cells.

The following NOS3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa233552	XM_024990490.1 Latest version!	Bos taurus nitric oxide synthase 3 (NOS3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa90649	XM_015470752.1 Latest version!	Bos taurus nitric oxide synthase 3 (NOS3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa01391	NM_181037.3 Latest version!	Bos taurus nitric oxide synthase 3 (endothelial cell) (NOS3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOS3 ( XM_024990490.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846258.1 Bos taurus nitric oxide synthase 3 (NOS3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa233552
Clone ID Related Accession (Same CDS sequence)	XM_024990490.1
Accession Version	XM_024990490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	nitric oxide synthase, endothelial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCACCT ACATCTGCAA CCACATCAAG TACGCCACCA ACCGCGGCAA CCTTCGCTCG 
GCCATCACAG TGTTCCCGCA GCGCGCCCCG GGCCGCGGAG ACTTCCGGAT CTGGAACAGC 
CAGCTGGTGC GCTACGCAGG CTACAGACAG CAGGATGGCT CTGTGCGTGG GGACCCAGCC 
AACGTGGAGA TCACGGAGCT CTGCATCCAG CACGGCTGGA CCCCCGGAAA CGGCCGCTTC 
GACGTGCTGC CCCTGCTGCT CCAGGCCCCA GACGAGGCTC CAGAGCTCTT TGTTCTGCCC 
CCCGAGCTGG TCCTTGAAGT GCCCCTGGAG CACCCCACAC TGGAGTGGTT CGCGGCCCTG 
GGCCTGCGAT GGTATGCCCT CCCGGCCGTG TCCAACATGC TGCTGGAAAT CGGGGGTCTG 
GAGTTCTCCG CGGCCCCCTT CAGCGGCTGG TACATGAGCA CGGAGATTGG CACGCGGAAC 
CTGTGTGACC CTCACCGCTA CAATATCCTG GAGGATGTGG CCGTCTGCAT GGACCTCGAC 
ACGCGGACCA CCTCGTCCCT GTGGAAGGAC AAGGCGGCCG TGGAGATCAA CCTGGCTGTG 
CTGCACAGCT TTCAGCTCGC CAAGGTGACC ATCGTGGACC ACCACGCCGC CACGGTGTCC 
TTCATGAAGC ACCTGGACAA CGAGCAGAAG GCCAGGGGGG GCTGCCCCGC CGACTGGGCC 
TGGATCGTGC CCCCCATCTC AGGCAGCCTC ACGCCCGTCT TCCACCAGGA GATGGTCAAC 
TACATCCTGT CCCCTGCCTT CCGCTACCAG CCAGACCCCT GGAAAGGGAG CGCGACCAAG 
GGCGCCGGCA TCACCAGGAA GAAGACCTTT AAGGAAGTGG CCAACGCGGT GAAGATCTCT 
GCCTCACTCA TGGGCACCCT GATGGCCAAG CGAGTGAAAG CAACCATCCT GTACGCCTCT 
GAGACCGGCC GGGCCCAGAG CTACGCTCAG CAGCTGGGGA GGCTCTTCCG GAAGGCCTTC 
GATCCCCGGG TCCTGTGCAT GGATGAGTAT GACGTGGTGT CCCTGGAGCA CGAGGCACTG 
GTGCTGGTGG TGACCAGCAC CTTTGGGAAT GGCGATCCCC CGGAGAATGG AGAGAGTTTT 
GCAGCTGCCC TGATGGAGAT GTCGGGGCCC TACAACAGCT CCCCACGGCC GGAACAGCAC 
AAGAGTTACA AGATCCGCTT CAACAGCGTC TCCTGCTCAG ACCCGCTGGT GTCCTCCTGG 
CGGCGGAAGA GAAAGGAGTC CAGCAACACA GACAGTGCGG GGGCCCTGGG GACCCTCAGG 
TTCTGTGTGT TCGGACTGGG CTCCCGGGCA TACCCCCACT TCTGCGCCTT CGCGCGAGCG 
GTGGACACCC GGCTGGAAGA GCTTGGAGGG GAGCGGCTGC TGCAGCTGGG CCAGGGCGAT 
GAGCTCTGCG GCCAGGAAGA GGCCTTCCGT GGTTGGGCAA AGGCGGCCTT CCAGGCCTCC 
TGTGAGACCT TCTGCGTTGG GGAGGAGGCC AAGGCCGCCG CCCAGGACAT CTTCAGCCCC 
AAACGGAGCT GGAAACGCCA GAGGTACCGG CTGAGCACCC AGGCCGAGGG CCTCCAGCTG 
CTGCCAGGCC TGATCCACGT GCACAGACGG AAGATGTTTC AGGCCACAGT CCTCTCGGTG 
GAAAATCTGC AAAGCAGCAA GTCCACCCGG GCCACCATCC TGGTGCGCCT GGACACTGCA 
GGCCAGGAGG GGCTGCAGTA CCAGCCGGGG GACCACATAG GCATCTGCCC GCCCAACCGG 
CCGGGCCTGG TGGAGGCGCT GCTGAGCCGC GTGGAGGACC CGCCACCGCC CACCGAGTCT 
GTGGCTGTGG AGCAGCTGGA GAAAGGCAGC CCAGGCGGCC CTCCTCCCAG CTGGGTGCGG 
GACCCACGGC TGCCCCCGTG CACGCTGCGC CAGGCTCTCA CCTTCTTCCT GGACATCACC 
TCCCCACCCA GCCCCCGGCT TCTCCGACTG CTCAGCACCC TGGCCGAAGA ACCCAGCGAG 
CAGCAGGAGC TTGAGACCCT CAGTCAGGAC CCCCGGCGCT ACGAGGAGTG GAAGTGGTTC 
CGCTGCCCCA CGCTGCTGGA GGTGCTGGAG CAGTTCCCGT CCGTGGCGCT GCCCGCCCCG 
CTGCTCCTCA CCCAGCTGCC CCTGCTGCAG CCCCGGTACT ACTCTGTCAG CTCGGCCCCC 
AACGCCCACC CCGGAGAGGT CCACCTCACA GTGGCCGTGC TGGCGTACAG GACCCAAGAT 
GGGCTGGGCC CCCTACACTA CGGGGTCTGC TCCACATGGC TGAGCCAGCT CAAGACTGGA 
GACCCCGTGC CCTGCTTCAT CAGGGGGGCT CCCTCCTTCC GGCTGCCGCC TGACCCCTAC 
GTGCCCTGCA TCCTCGTGGG CCCTGGCACT GGCATCGCCC CCTTCCGGGG ATTTTGGCAG 
GAGAGGCTGC ATGACATTGA GAGCAAAGGG CTGCAGCCCG CCCCCATGAC CCTGGTGTTC 
GGCTGCCGCT GCTCCCAACT CGACCATCTC TACCGCGACG AGGTGCAGGA CGCCCAGGAG 
CGCGGGGTGT TTGGCCGCGT CCTCACCGCC TTCTCCCGGG AACCTGACAG CCCCAAGACC 
TACGTACAGG ACATCCTGAG AACCGAGCTG GCTGCCGAGG TGCACCGCGT GCTGTGCCTC 
GAGCGGGGCC ACATGTTTGT CTGCGGCGAT GTCACTATGG CAACCAGCGT CCTGCAGACG 
GTGCAGCGCA TCTTGGCGAC AGAGGGCGAC ATGGAGCTGG ACGAGGCGGG CGACGTCATC 
GGCGTGCTGC GGGATCAGCA ACGCTATCAC GAGGACATTT TCGGCCTCAC GCTGCGCACC 
CAGGAGGTGA CAAGCCGTAT ACGTACCCAG AGCTTTTCCC TGCAGGAGCG GCATCTGCGG 
GGCGCGGTGC CCTGGGCCTT CGACCCGCCC GGCCCAGACA CCCCCGGCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846258.1
CDS	645..3638
Translation	MFTYICNHIKYATNRGNLRSAITVFPQRAPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDEAPELFVLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFSAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINLAVLHSFQLAKVTIVDHHAATVSFMKHLDNEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYILSPAFRYQPDPWKGSATKGAGITRKKTFKEVANAVKISASLMGTLMAKRVKATILYASETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHEALVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSVSCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAKAAFQASCETFCVGEEAKAAAQDIFSPKRSWKRQRYRLSTQAEGLQLLPGLIHVHRRKMFQATVLSVENLQSSKSTRATILVRLDTAGQEGLQYQPGDHIGICPPNRPGLVEALLSRVEDPPPPTESVAVEQLEKGSPGGPPPSWVRDPRLPPCTLRQALTFFLDITSPPSPRLLRLLSTLAEEPSEQQELETLSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPNAHPGEVHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKTGDPVPCFIRGAPSFRLPPDPYVPCILVGPGTGIAPFRGFWQERLHDIESKGLQPAPMTLVFGCRCSQLDHLYRDEVQDAQERGVFGRVLTAFSREPDSPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATSVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERHLRGAVPWAFDPPGPDTPGP

Target ORF information:

RefSeq Version	XM_024990490.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nitric oxide synthase 3 (NOS3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990490.1

ORF Insert Sequence:

ATGTTCACCT ACATCTGCAA CCACATCAAG TACGCCACCA ACCGCGGCAA CCTTCGCTCG 
GCCATCACAG TGTTCCCGCA GCGCGCCCCG GGCCGCGGAG ACTTCCGGAT CTGGAACAGC 
CAGCTGGTGC GCTACGCAGG CTACAGACAG CAGGATGGCT CTGTGCGTGG GGACCCAGCC 
AACGTGGAGA TCACGGAGCT CTGCATCCAG CACGGCTGGA CCCCCGGAAA CGGCCGCTTC 
GACGTGCTGC CCCTGCTGCT CCAGGCCCCA GACGAGGCTC CAGAGCTCTT TGTTCTGCCC 
CCCGAGCTGG TCCTTGAAGT GCCCCTGGAG CACCCCACAC TGGAGTGGTT CGCGGCCCTG 
GGCCTGCGAT GGTATGCCCT CCCGGCCGTG TCCAACATGC TGCTGGAAAT CGGGGGTCTG 
GAGTTCTCCG CGGCCCCCTT CAGCGGCTGG TACATGAGCA CGGAGATTGG CACGCGGAAC 
CTGTGTGACC CTCACCGCTA CAATATCCTG GAGGATGTGG CCGTCTGCAT GGACCTCGAC 
ACGCGGACCA CCTCGTCCCT GTGGAAGGAC AAGGCGGCCG TGGAGATCAA CCTGGCTGTG 
CTGCACAGCT TTCAGCTCGC CAAGGTGACC ATCGTGGACC ACCACGCCGC CACGGTGTCC 
TTCATGAAGC ACCTGGACAA CGAGCAGAAG GCCAGGGGGG GCTGCCCCGC CGACTGGGCC 
TGGATCGTGC CCCCCATCTC AGGCAGCCTC ACGCCCGTCT TCCACCAGGA GATGGTCAAC 
TACATCCTGT CCCCTGCCTT CCGCTACCAG CCAGACCCCT GGAAAGGGAG CGCGACCAAG 
GGCGCCGGCA TCACCAGGAA GAAGACCTTT AAGGAAGTGG CCAACGCGGT GAAGATCTCT 
GCCTCACTCA TGGGCACCCT GATGGCCAAG CGAGTGAAAG CAACCATCCT GTACGCCTCT 
GAGACCGGCC GGGCCCAGAG CTACGCTCAG CAGCTGGGGA GGCTCTTCCG GAAGGCCTTC 
GATCCCCGGG TCCTGTGCAT GGATGAGTAT GACGTGGTGT CCCTGGAGCA CGAGGCACTG 
GTGCTGGTGG TGACCAGCAC CTTTGGGAAT GGCGATCCCC CGGAGAATGG AGAGAGTTTT 
GCAGCTGCCC TGATGGAGAT GTCGGGGCCC TACAACAGCT CCCCACGGCC GGAACAGCAC 
AAGAGTTACA AGATCCGCTT CAACAGCGTC TCCTGCTCAG ACCCGCTGGT GTCCTCCTGG 
CGGCGGAAGA GAAAGGAGTC CAGCAACACA GACAGTGCGG GGGCCCTGGG GACCCTCAGG 
TTCTGTGTGT TCGGACTGGG CTCCCGGGCA TACCCCCACT TCTGCGCCTT CGCGCGAGCG 
GTGGACACCC GGCTGGAAGA GCTTGGAGGG GAGCGGCTGC TGCAGCTGGG CCAGGGCGAT 
GAGCTCTGCG GCCAGGAAGA GGCCTTCCGT GGTTGGGCAA AGGCGGCCTT CCAGGCCTCC 
TGTGAGACCT TCTGCGTTGG GGAGGAGGCC AAGGCCGCCG CCCAGGACAT CTTCAGCCCC 
AAACGGAGCT GGAAACGCCA GAGGTACCGG CTGAGCACCC AGGCCGAGGG CCTCCAGCTG 
CTGCCAGGCC TGATCCACGT GCACAGACGG AAGATGTTTC AGGCCACAGT CCTCTCGGTG 
GAAAATCTGC AAAGCAGCAA GTCCACCCGG GCCACCATCC TGGTGCGCCT GGACACTGCA 
GGCCAGGAGG GGCTGCAGTA CCAGCCGGGG GACCACATAG GCATCTGCCC GCCCAACCGG 
CCGGGCCTGG TGGAGGCGCT GCTGAGCCGC GTGGAGGACC CGCCACCGCC CACCGAGTCT 
GTGGCTGTGG AGCAGCTGGA GAAAGGCAGC CCAGGCGGCC CTCCTCCCAG CTGGGTGCGG 
GACCCACGGC TGCCCCCGTG CACGCTGCGC CAGGCTCTCA CCTTCTTCCT GGACATCACC 
TCCCCACCCA GCCCCCGGCT TCTCCGACTG CTCAGCACCC TGGCCGAAGA ACCCAGCGAG 
CAGCAGGAGC TTGAGACCCT CAGTCAGGAC CCCCGGCGCT ACGAGGAGTG GAAGTGGTTC 
CGCTGCCCCA CGCTGCTGGA GGTGCTGGAG CAGTTCCCGT CCGTGGCGCT GCCCGCCCCG 
CTGCTCCTCA CCCAGCTGCC CCTGCTGCAG CCCCGGTACT ACTCTGTCAG CTCGGCCCCC 
AACGCCCACC CCGGAGAGGT CCACCTCACA GTGGCCGTGC TGGCGTACAG GACCCAAGAT 
GGGCTGGGCC CCCTACACTA CGGGGTCTGC TCCACATGGC TGAGCCAGCT CAAGACTGGA 
GACCCCGTGC CCTGCTTCAT CAGGGGGGCT CCCTCCTTCC GGCTGCCGCC TGACCCCTAC 
GTGCCCTGCA TCCTCGTGGG CCCTGGCACT GGCATCGCCC CCTTCCGGGG ATTTTGGCAG 
GAGAGGCTGC ATGACATTGA GAGCAAAGGG CTGCAGCCCG CCCCCATGAC CCTGGTGTTC 
GGCTGCCGCT GCTCCCAACT CGACCATCTC TACCGCGACG AGGTGCAGGA CGCCCAGGAG 
CGCGGGGTGT TTGGCCGCGT CCTCACCGCC TTCTCCCGGG AACCTGACAG CCCCAAGACC 
TACGTACAGG ACATCCTGAG AACCGAGCTG GCTGCCGAGG TGCACCGCGT GCTGTGCCTC 
GAGCGGGGCC ACATGTTTGT CTGCGGCGAT GTCACTATGG CAACCAGCGT CCTGCAGACG 
GTGCAGCGCA TCTTGGCGAC AGAGGGCGAC ATGGAGCTGG ACGAGGCGGG CGACGTCATC 
GGCGTGCTGC GGGATCAGCA ACGCTATCAC GAGGACATTT TCGGCCTCAC GCTGCGCACC 
CAGGAGGTGA CAAGCCGTAT ACGTACCCAG AGCTTTTCCC TGCAGGAGCG GCATCTGCGG 
GGCGCGGTGC CCTGGGCCTT CGACCCGCCC GGCCCAGACA CCCCCGGCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS3 ( XM_015470752.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326238.1 Bos taurus nitric oxide synthase 3 (NOS3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa90649
Clone ID Related Accession (Same CDS sequence)	XM_015470752.1
Accession Version	XM_015470752.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	nitric oxide synthase, endothelial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCACCT ACATCTGCAA CCACATCAAG TACGCCACCA ACCGCGGCAA CCTTCGCTCG 
GCCATCACAG TGTTCCCGCA GCGCGCCCCG GGCCGCGGAG ACTTCCGGAT CTGGAACAGC 
CAGCTGGTGC GCTACGCAGG CTACAGACAG CAGGATGGCT CTGTGCGTGG GGACCCAGCC 
AATGTGGAGA TCACGGAGCT CTGCATCCAG CACGGCTGGA CCCCCGGAAA CGGCCGCTTC 
GACGTGCTGC CCCTGCTGCT CCAGGCCCCA GACGAGGCTC CAGAGCTCTT TGTTCTGCCC 
CCCGAGCTGG TCCTTGAAGT GCCCCTGGAG CACCCCACAC TGGAGTGGTT TGCGGCCCTG 
GGCCTGCGAT GGTATGCCCT CCCGGCCGTG TCCAACATGC TGCTGGAAAT CGGGGGTCTG 
GAGTTCTCCG CGGCCCCCTT CAGCGGCTGG TACATGAGCA CGGAGATTGG CACGCGGAAC 
CTGTGTGACC CTCACCGCTA CAATATCCTG GAGGATGTGG CCGTCTGCAT GGACCTCGAC 
ACGCGGACCA CCTCGTCCCT GTGGAAGGAC AAGGCGGCCG TGGAGATCAA CCTGGCTGTG 
CTGCACAGCT TTCAGCTCGC CAAGGTGACC ATCGTGGACC ACCACGCCGC CACGGTGTCC 
TTCATGAAGC ACCTGGACAA CGAGCAGAAG GCCAGGGGGG GCTGCCCCGC CGACTGGGCC 
TGGATCGTGC CCCCCATCTC AGGCAGCCTC ACGCCCGTCT TCCACCAGGA GATGGTCAAC 
TACATCCTGT CCCCTGCCTT CCGCTACCAG CCAGACCCCT GGAAAGGGAG CGCGACCAAG 
GGCGCAGGCA TCACCAGGAA GAAGACCTTT AAGGAAGTGG CCAACGCGGT GAAGATCTCT 
GCCTCACTCA TGGGCACCCT GATGGCCAAG CGAGTGAAAG CAACCATCCT GTACGCCTCT 
GAGACCGGCC GGGCCCAGAG CTACGCTCAG CAGCTGGGGA GGCTCTTCCG GAAGGCCTTC 
GATCCCCGGG TCCTGTGCAT GGATGAGTAT GACGTGGTGT CCCTGGAGCA CGAGGCACTG 
GTGCTGGTGG TGACCAGCAC CTTTGGGAAT GGCGATCCCC CGGAGAATGG AGAGAGTTTT 
GCAGCTGCCC TGATGGAGAT GTCGGGGCCC TACAACAGCT CCCCACGGCC GGAACAGCAC 
AAGAGTTACA AGATCCGCTT CAACAGCGTC TCCTGCTCAG ACCCGCTGGT GTCCTCCTGG 
CGGCGGAAGA GAAAGGAGTC CAGCAACACA GACAGTGCGG GGGCCCTGGG GACCCTCAGG 
TTCTGTGTGT TCGGACTGGG CTCCCGGGCA TACCCCCACT TCTGCGCCTT CGCGCGAGCG 
GTGGACACCC GGCTGGAAGA GCTTGGAGGG GAGCGGCTGC TGCAGCTGGG CCAGGGCGAT 
GAGCTCTGCG GCCAGGAAGA GGCCTTCCGT GGTTGGGCAA AGGCGGCCTT CCAGGCCTCC 
TGTGAGACCT TCTGCGTTGG GGAGGAGGCC AAGGCCGCCG CCCAGGACAT CTTCAGCCCC 
AAACGGAGCT GGAAACGCCA GAGGTACCGG CTGAGCACCC AGGCCGAGGG CCTCCAGCTG 
CTGCCAGGCC TGATCCACGT GCACAGACGG AAGATGTTTC AGGCCACAGT CCTCTCGGTG 
GAAAATCTGC AAAGCAGCAA GTCCACCCGG GCCACCATCC TGGTGCGCCT GGACACTGCA 
GGCCAGGAGG GGCTGCAGTA CCAGCCGGGG GACCACATAG GCATCTGCCC GCCCAACCGG 
CCGGGCCTGG TGGAGGCGCT GCTGAGCCGC GTGGAGGACC CGCCACCGCC CACCGAGTCT 
GTGGCTGTGG AGCAGCTGGA GAAAGGCAGC CCAGGCGGCC CTCCTCCCAG CTGGGTGCGG 
GACCCACGGC TGCCCCCGTG CACGCTGCGC CAGGCTCTCA CCTTCTTCCT GGACATCACC 
TCCCCACCCA GCCCCCGGCT TCTCCGACTG CTCAGCACCC TGGCCGAAGA ACCCAGCGAG 
CAGCAGGAGC TTGAGACCCT CAGTCAGGAC CCCCGGCGCT ACGAGGAGTG GAAGTGGTTC 
CGCTGCCCCA CGCTGCTGGA GGTGCTGGAG CAGTTCCCGT CCGTGGCGCT GCCCGCCCCG 
CTGCTCCTCA CCCAGCTGCC CCTGCTGCAG CCCCGGTACT ACTCTGTCAG CTCGGCCCCC 
AACGCCCACC CCGGAGAGGT CCACCTCACA GTGGCCGTGC TGGCGTACAG GACCCAAGAT 
GGGCTGGGCC CCCTACACTA CGGGGTCTGC TCCACATGGC TGAGCCAGCT CAAGACTGGA 
GACCCCGTGC CCTGCTTCAT CAGGGGGGCT CCCTCCTTCC GGCTGCCGCC TGACCCCTAC 
GTGCCCTGCA TCCTCGTGGG CCCTGGCACT GGCATCGCCC CCTTCCGGGG ATTTTGGCAG 
GAGAGGCTGC ATGACATTGA GAGCAAAGGG CTGCAGCCCG CCCCCATGAC CCTGGTGTTC 
GGCTGCCGCT GCTCCCAACT CGACCATCTC TACCGCGACG AGGTGCAGGA CGCCCAGGAG 
CGCGGGGTGT TTGGCCGCGT CCTCACCGCC TTCTCCCGGG AACCTGACAG CCCCAAGACC 
TACGTACAGG ACATCCTGAG AACCGAGCTG GCTGCCGAGG TGCACCGCGT GCTGTGCCTC 
GAGCGGGGCC ACATGTTTGT CTGCGGCGAT GTCACTATGG CAACCAGCGT CCTGCAGACG 
GTGCAGCGCA TCTTGGCGAC AGAGGGCGAC ATGGAGCTGG ACGAGGCGGG CGACGTCATC 
GGCGTGCTGC GGGATCAGCA ACGCTATCAC GAGGACATTT TCGGCCTCAC GCTGCGCACC 
CAGGAGGTGA CAAGCCGTAT ACGTACCCAG AGCTTTTCCC TGCAGGAGCG GCATCTGCGG 
GGCGCGGTGC CCTGGGCCTT CGACCCGCCC GGCCCAGACA CCCCCGGCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326238.1
CDS	940..3933
Translation	MFTYICNHIKYATNRGNLRSAITVFPQRAPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDEAPELFVLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFSAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINLAVLHSFQLAKVTIVDHHAATVSFMKHLDNEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYILSPAFRYQPDPWKGSATKGAGITRKKTFKEVANAVKISASLMGTLMAKRVKATILYASETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHEALVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSVSCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAKAAFQASCETFCVGEEAKAAAQDIFSPKRSWKRQRYRLSTQAEGLQLLPGLIHVHRRKMFQATVLSVENLQSSKSTRATILVRLDTAGQEGLQYQPGDHIGICPPNRPGLVEALLSRVEDPPPPTESVAVEQLEKGSPGGPPPSWVRDPRLPPCTLRQALTFFLDITSPPSPRLLRLLSTLAEEPSEQQELETLSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPNAHPGEVHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKTGDPVPCFIRGAPSFRLPPDPYVPCILVGPGTGIAPFRGFWQERLHDIESKGLQPAPMTLVFGCRCSQLDHLYRDEVQDAQERGVFGRVLTAFSREPDSPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATSVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERHLRGAVPWAFDPPGPDTPGP

Target ORF information:

RefSeq Version	XM_015470752.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nitric oxide synthase 3 (NOS3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470752.1

ORF Insert Sequence:

ATGTTCACCT ACATCTGCAA CCACATCAAG TACGCCACCA ACCGCGGCAA CCTTCGCTCG 
GCCATCACAG TGTTCCCGCA GCGCGCCCCG GGCCGCGGAG ACTTCCGGAT CTGGAACAGC 
CAGCTGGTGC GCTACGCAGG CTACAGACAG CAGGATGGCT CTGTGCGTGG GGACCCAGCC 
AATGTGGAGA TCACGGAGCT CTGCATCCAG CACGGCTGGA CCCCCGGAAA CGGCCGCTTC 
GACGTGCTGC CCCTGCTGCT CCAGGCCCCA GACGAGGCTC CAGAGCTCTT TGTTCTGCCC 
CCCGAGCTGG TCCTTGAAGT GCCCCTGGAG CACCCCACAC TGGAGTGGTT TGCGGCCCTG 
GGCCTGCGAT GGTATGCCCT CCCGGCCGTG TCCAACATGC TGCTGGAAAT CGGGGGTCTG 
GAGTTCTCCG CGGCCCCCTT CAGCGGCTGG TACATGAGCA CGGAGATTGG CACGCGGAAC 
CTGTGTGACC CTCACCGCTA CAATATCCTG GAGGATGTGG CCGTCTGCAT GGACCTCGAC 
ACGCGGACCA CCTCGTCCCT GTGGAAGGAC AAGGCGGCCG TGGAGATCAA CCTGGCTGTG 
CTGCACAGCT TTCAGCTCGC CAAGGTGACC ATCGTGGACC ACCACGCCGC CACGGTGTCC 
TTCATGAAGC ACCTGGACAA CGAGCAGAAG GCCAGGGGGG GCTGCCCCGC CGACTGGGCC 
TGGATCGTGC CCCCCATCTC AGGCAGCCTC ACGCCCGTCT TCCACCAGGA GATGGTCAAC 
TACATCCTGT CCCCTGCCTT CCGCTACCAG CCAGACCCCT GGAAAGGGAG CGCGACCAAG 
GGCGCAGGCA TCACCAGGAA GAAGACCTTT AAGGAAGTGG CCAACGCGGT GAAGATCTCT 
GCCTCACTCA TGGGCACCCT GATGGCCAAG CGAGTGAAAG CAACCATCCT GTACGCCTCT 
GAGACCGGCC GGGCCCAGAG CTACGCTCAG CAGCTGGGGA GGCTCTTCCG GAAGGCCTTC 
GATCCCCGGG TCCTGTGCAT GGATGAGTAT GACGTGGTGT CCCTGGAGCA CGAGGCACTG 
GTGCTGGTGG TGACCAGCAC CTTTGGGAAT GGCGATCCCC CGGAGAATGG AGAGAGTTTT 
GCAGCTGCCC TGATGGAGAT GTCGGGGCCC TACAACAGCT CCCCACGGCC GGAACAGCAC 
AAGAGTTACA AGATCCGCTT CAACAGCGTC TCCTGCTCAG ACCCGCTGGT GTCCTCCTGG 
CGGCGGAAGA GAAAGGAGTC CAGCAACACA GACAGTGCGG GGGCCCTGGG GACCCTCAGG 
TTCTGTGTGT TCGGACTGGG CTCCCGGGCA TACCCCCACT TCTGCGCCTT CGCGCGAGCG 
GTGGACACCC GGCTGGAAGA GCTTGGAGGG GAGCGGCTGC TGCAGCTGGG CCAGGGCGAT 
GAGCTCTGCG GCCAGGAAGA GGCCTTCCGT GGTTGGGCAA AGGCGGCCTT CCAGGCCTCC 
TGTGAGACCT TCTGCGTTGG GGAGGAGGCC AAGGCCGCCG CCCAGGACAT CTTCAGCCCC 
AAACGGAGCT GGAAACGCCA GAGGTACCGG CTGAGCACCC AGGCCGAGGG CCTCCAGCTG 
CTGCCAGGCC TGATCCACGT GCACAGACGG AAGATGTTTC AGGCCACAGT CCTCTCGGTG 
GAAAATCTGC AAAGCAGCAA GTCCACCCGG GCCACCATCC TGGTGCGCCT GGACACTGCA 
GGCCAGGAGG GGCTGCAGTA CCAGCCGGGG GACCACATAG GCATCTGCCC GCCCAACCGG 
CCGGGCCTGG TGGAGGCGCT GCTGAGCCGC GTGGAGGACC CGCCACCGCC CACCGAGTCT 
GTGGCTGTGG AGCAGCTGGA GAAAGGCAGC CCAGGCGGCC CTCCTCCCAG CTGGGTGCGG 
GACCCACGGC TGCCCCCGTG CACGCTGCGC CAGGCTCTCA CCTTCTTCCT GGACATCACC 
TCCCCACCCA GCCCCCGGCT TCTCCGACTG CTCAGCACCC TGGCCGAAGA ACCCAGCGAG 
CAGCAGGAGC TTGAGACCCT CAGTCAGGAC CCCCGGCGCT ACGAGGAGTG GAAGTGGTTC 
CGCTGCCCCA CGCTGCTGGA GGTGCTGGAG CAGTTCCCGT CCGTGGCGCT GCCCGCCCCG 
CTGCTCCTCA CCCAGCTGCC CCTGCTGCAG CCCCGGTACT ACTCTGTCAG CTCGGCCCCC 
AACGCCCACC CCGGAGAGGT CCACCTCACA GTGGCCGTGC TGGCGTACAG GACCCAAGAT 
GGGCTGGGCC CCCTACACTA CGGGGTCTGC TCCACATGGC TGAGCCAGCT CAAGACTGGA 
GACCCCGTGC CCTGCTTCAT CAGGGGGGCT CCCTCCTTCC GGCTGCCGCC TGACCCCTAC 
GTGCCCTGCA TCCTCGTGGG CCCTGGCACT GGCATCGCCC CCTTCCGGGG ATTTTGGCAG 
GAGAGGCTGC ATGACATTGA GAGCAAAGGG CTGCAGCCCG CCCCCATGAC CCTGGTGTTC 
GGCTGCCGCT GCTCCCAACT CGACCATCTC TACCGCGACG AGGTGCAGGA CGCCCAGGAG 
CGCGGGGTGT TTGGCCGCGT CCTCACCGCC TTCTCCCGGG AACCTGACAG CCCCAAGACC 
TACGTACAGG ACATCCTGAG AACCGAGCTG GCTGCCGAGG TGCACCGCGT GCTGTGCCTC 
GAGCGGGGCC ACATGTTTGT CTGCGGCGAT GTCACTATGG CAACCAGCGT CCTGCAGACG 
GTGCAGCGCA TCTTGGCGAC AGAGGGCGAC ATGGAGCTGG ACGAGGCGGG CGACGTCATC 
GGCGTGCTGC GGGATCAGCA ACGCTATCAC GAGGACATTT TCGGCCTCAC GCTGCGCACC 
CAGGAGGTGA CAAGCCGTAT ACGTACCCAG AGCTTTTCCC TGCAGGAGCG GCATCTGCGG 
GGCGCGGTGC CCTGGGCCTT CGACCCGCCC GGCCCAGACA CCCCCGGCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS3 ( NM_181037.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_851380.2 Bos taurus nitric oxide synthase 3 (endothelial cell) (NOS3), mRNA.

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CloneID	OBa01391
Clone ID Related Accession (Same CDS sequence)	NM_181037.3
Accession Version	NM_181037.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(cattle)
Product	nitric oxide synthase, endothelial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03032796.1, AAFC03082583.1 and AAFC03082584.1. On Jan 6, 2011 this sequence version replaced NM_181037.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M99057.1, M89952.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01093727, SAMN01093728 [ECO:0000348] ##Evidence-Data-END##

ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTCG GGCTATGCGG CAAGCAGGGC CCAGCCTCCC CGGCACCTGA GCCCAGCCGG 
GCCCCCGCAC CCGCCACCCC GCACGCGCCA GACCACAGCC CAGCTCCCAA CAGCCCCACG 
CTGACCCGGC CTCCGGAGGG GCCCAAGTTC CCTCGCGTGA AGAACTGGGA GCTGGGGAGC 
ATCACCTACG ACACTCTGTG CGCGCAGTCC CAACAGGACG GGCCCTGCAC TCCCAGGCGC 
TGCCTGGGCT CCCTGGTGTT GCCCCGGAAA CTGCAGACCC GGCCCTCCCC GGGACCTCCA 
CCCGCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATC 
AAGAGGAGCG GCTCCCAGGC TCACGAGGAG CGGCTTCAGG AGGTGGAGGC CGAGGTGGCA 
TCCACGGGCA CCTACCACCT CCGAGAGAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG 
CGCAACGCAC CCCGCTGCGT GGGCCGCATC CAGTGGGGGA AGCTGCAGGT GTTTGATGCC 
CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC 
ACCAACCGCG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CGCAGCGCGC CCCGGGCCGC 
GGAGACTTCC GGATCTGGAA CAGCCAGCTG GTGCGCTACG CAGGCTACAG ACAGCAGGAT 
GGCTCTGTGC GTGGGGACCC AGCCAATGTG GAGATCACGG AGCTCTGCAT CCAGCACGGC 
TGGACCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGACGAG 
GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTTG AAGTGCCCCT GGAGCACCCC 
ACACTGGAGT GGTTTGCGGC CCTGGGCCTG CGATGGTATG CCCTCCCGGC CGTGTCCAAC 
ATGCTGCTGG AAATCGGGGG TCTGGAGTTC TCCGCGGCCC CCTTCAGCGG CTGGTACATG 
AGCACGGAGA TTGGCACGCG GAACCTGTGT GACCCTCACC GCTACAATAT CCTGGAGGAT 
GTGGCCGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCCTGTGGAA GGACAAGGCG 
GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC TCGCCAAGGT GACCATCGTG 
GACCACCACG CCGCCACGGT GTCCTTCATG AAGCACCTGG ACAACGAGCA GAAGGCCAGG 
GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCAGGCAG CCTCACGCCC 
GTCTTCCACC AGGAGATGGT CAACTACATC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC 
CCCTGGAAAG GGAGCGCGAC CAAGGGCGCA GGCATCACCA GGAAGAAGAC CTTTAAGGAA 
GTGGCCAACG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG 
AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGCTACGC TCAGCAGCTG 
GGGAGGCTCT TCCGGAAGGC CTTCGATCCC CGGGTCCTGT GCATGGATGA GTATGACGTG 
GTGTCCCTGG AGCACGAGGC ACTGGTGCTG GTGGTGACCA GCACCTTTGG GAATGGCGAT 
CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC 
AGCTCCCCGC GGCCGGAACA GCACAAGAGT TACAAGATCC GCTTCAACAG CGTCTCCTGC 
TCAGACCCGC TGGTGTCCTC CTGGCGGCGG AAGAGAAAGG AGTCCAGCAA CACAGACAGT 
GCGGGGGCCC TGGGGACCCT CAGGTTCTGT GTGTTCGGAC TGGGCTCCCG GGCATACCCC 
CACTTCTGCG CCTTCGCGCG AGCGGTGGAC ACCCGGCTGG AAGAGCTTGG AGGGGAGCGG 
CTGCTGCAGC TGGGCCAGGG CGATGAGCTC TGCGGCCAGG AAGAGGCCTT CCGTGGTTGG 
GCAAAGGCGG CCTTCCAGGC CTCCTGTGAG ACCTTCTGCG TTGGGGAGGA GGCCAAGGCC 
GCCGCCCAGG ACATCTTCAG CCCCAAACGG AGCTGGAAAC GCCAGAGGTA CCGGCTGAGC 
ACCCAGGCCG AGGGCCTCCA GCTGCTGCCA GGCCTGATCC ACGTGCACAG ACGGAAGATG 
TTTCAGGCCA CAGTCCTCTC GGTGGAAAAT CTGCAAAGCA GCAAGTCCAC CCGGGCCACC 
ATCCTGGTGC GCCTGGACAC TGCAGGCCAG GAGGGGCTGC AGTACCAGCC GGGGGACCAC 
ATAGGCATCT GCCCGCCCAA CCGGCCGGGC CTGGTGGAGG CGCTGCTGAG CCGCGTGGAG 
GACCCGCCAC CGCCCACCGA GTCTGTGGCT GTGGAGCAGC TGGAGAAAGG CAGCCCAGGC 
GGCCCTCCTC CCAGCTGGGT GCGGGACCCA CGGCTGCCCC CGTGCACGCT GCGCCAGGCT 
CTCACCTTCT TCCTGGACAT CACCTCCCCA CCCAGCCCCC GGCTTCTCCG ACTGCTCAGC 
ACCCTGGCCG AAGAACCCAG CGAGCAGCAG GAGCTTGAGA CCCTCAGTCA GGACCCCCGG 
CGCTACGAGG AGTGGAAGTG GTTCCGCTGC CCCACGCTGC TGGAGGTGCT GGAGCAGTTC 
CCGTCCGTGG CGCTGCCCGC CCCGCTGCTC CTCACCCAGC TGCCCCTGCT GCAGCCCCGG 
TACTACTCTG TCAGCTCGGC CCCCAACGCC CACCCCGGAG AGGTCCACCT CACAGTGGCC 
GTGCTGGCGT ACAGGACCCA AGATGGGCTG GGCCCCCTAC ACTACGGGGT CTGCTCCACA 
TGGCTGAGCC AGCTCAAGAC TGGAGACCCC GTGCCCTGCT TCATCAGGGG GGCTCCCTCC 
TTCCGGCTGC CGCCTGACCC CTACGTGCCC TGCATCCTCG TGGGCCCTGG CACTGGCATC 
GCCCCCTTCC GGGGATTTTG GCAGGAGAGG CTGCATGACA TTGAGAGCAA AGGGCTGCAG 
CCCGCCCCCA TGACCCTGGT GTTCGGCTGC CGCTGCTCCC AACTCGACCA TCTCTACCGC 
GACGAGGTGC AGGACGCCCA GGAGCGCGGG GTGTTTGGCC GCGTCCTCAC CGCCTTCTCC 
CGGGAACCTG ACAGCCCCAA GACCTACGTA CAGGACATCC TGAGAACCGA GCTGGCTGCC 
GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTCTGCGG CGATGTCACT 
ATGGCAACCA GCGTCCTGCA GACGGTGCAG CGCATCTTGG CGACAGAGGG CGACATGGAG 
CTGGACGAGG CGGGCGACGT CATCGGCGTG CTGCGGGATC AGCAACGCTA TCACGAGGAC 
ATTTTCGGCC TCACGCTGCG CACCCAGGAG GTGACAAGCC GTATACGTAC CCAGAGCTTT 
TCCCTGCAGG AGCGGCATCT GCGGGGCGCG GTGCCCTGGG CCTTCGACCC GCCCGGCCCA 
GACACCCCCG GCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851380.2
CDS	44..3661
Translation	MGNLKSVGQEPGPPCGLGLGLGLGLCGKQGPASPAPEPSRAPAPATPHAPDHSPAPNSPTLTRPPEGPKFPRVKNWELGSITYDTLCAQSQQDGPCTPRRCLGSLVLPRKLQTRPSPGPPPAEQLLSQARDFINQYYSSIKRSGSQAHEERLQEVEAEVASTGTYHLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCSSAQEMFTYICNHIKYATNRGNLRSAITVFPQRAPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDEAPELFVLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFSAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINLAVLHSFQLAKVTIVDHHAATVSFMKHLDNEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYILSPAFRYQPDPWKGSATKGAGITRKKTFKEVANAVKISASLMGTLMAKRVKATILYASETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHEALVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSVSCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAKAAFQASCETFCVGEEAKAAAQDIFSPKRSWKRQRYRLSTQAEGLQLLPGLIHVHRRKMFQATVLSVENLQSSKSTRATILVRLDTAGQEGLQYQPGDHIGICPPNRPGLVEALLSRVEDPPPPTESVAVEQLEKGSPGGPPPSWVRDPRLPPCTLRQALTFFLDITSPPSPRLLRLLSTLAEEPSEQQELETLSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPNAHPGEVHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKTGDPVPCFIRGAPSFRLPPDPYVPCILVGPGTGIAPFRGFWQERLHDIESKGLQPAPMTLVFGCRCSQLDHLYRDEVQDAQERGVFGRVLTAFSREPDSPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATSVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERHLRGAVPWAFDPPGPDTPGP

Target ORF information:

RefSeq Version	NM_181037.3
Organism	Bos taurus(cattle)
Definition	Bos taurus nitric oxide synthase 3 (endothelial cell) (NOS3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181037.3

ORF Insert Sequence:

ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CCTGCGGCCT GGGGCTGGGG 
CTGGGCCTCG GGCTATGCGG CAAGCAGGGC CCAGCCTCCC CGGCACCTGA GCCCAGCCGG 
GCCCCCGCAC CCGCCACCCC GCACGCGCCA GACCACAGCC CAGCTCCCAA CAGCCCCACG 
CTGACCCGGC CTCCGGAGGG GCCCAAGTTC CCTCGCGTGA AGAACTGGGA GCTGGGGAGC 
ATCACCTACG ACACTCTGTG CGCGCAGTCC CAACAGGACG GGCCCTGCAC TCCCAGGCGC 
TGCCTGGGCT CCCTGGTGTT GCCCCGGAAA CTGCAGACCC GGCCCTCCCC GGGACCTCCA 
CCCGCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATC 
AAGAGGAGCG GCTCCCAGGC TCACGAGGAG CGGCTTCAGG AGGTGGAGGC CGAGGTGGCA 
TCCACGGGCA CCTACCACCT CCGAGAGAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG 
CGCAACGCAC CCCGCTGCGT GGGCCGCATC CAGTGGGGGA AGCTGCAGGT GTTTGATGCC 
CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC 
ACCAACCGCG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CGCAGCGCGC CCCGGGCCGC 
GGAGACTTCC GGATCTGGAA CAGCCAGCTG GTGCGCTACG CAGGCTACAG ACAGCAGGAT 
GGCTCTGTGC GTGGGGACCC AGCCAATGTG GAGATCACGG AGCTCTGCAT CCAGCACGGC 
TGGACCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGACGAG 
GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTTG AAGTGCCCCT GGAGCACCCC 
ACACTGGAGT GGTTTGCGGC CCTGGGCCTG CGATGGTATG CCCTCCCGGC CGTGTCCAAC 
ATGCTGCTGG AAATCGGGGG TCTGGAGTTC TCCGCGGCCC CCTTCAGCGG CTGGTACATG 
AGCACGGAGA TTGGCACGCG GAACCTGTGT GACCCTCACC GCTACAATAT CCTGGAGGAT 
GTGGCCGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCCTGTGGAA GGACAAGGCG 
GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC TCGCCAAGGT GACCATCGTG 
GACCACCACG CCGCCACGGT GTCCTTCATG AAGCACCTGG ACAACGAGCA GAAGGCCAGG 
GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCAGGCAG CCTCACGCCC 
GTCTTCCACC AGGAGATGGT CAACTACATC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC 
CCCTGGAAAG GGAGCGCGAC CAAGGGCGCA GGCATCACCA GGAAGAAGAC CTTTAAGGAA 
GTGGCCAACG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG 
AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGCTACGC TCAGCAGCTG 
GGGAGGCTCT TCCGGAAGGC CTTCGATCCC CGGGTCCTGT GCATGGATGA GTATGACGTG 
GTGTCCCTGG AGCACGAGGC ACTGGTGCTG GTGGTGACCA GCACCTTTGG GAATGGCGAT 
CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC 
AGCTCCCCGC GGCCGGAACA GCACAAGAGT TACAAGATCC GCTTCAACAG CGTCTCCTGC 
TCAGACCCGC TGGTGTCCTC CTGGCGGCGG AAGAGAAAGG AGTCCAGCAA CACAGACAGT 
GCGGGGGCCC TGGGGACCCT CAGGTTCTGT GTGTTCGGAC TGGGCTCCCG GGCATACCCC 
CACTTCTGCG CCTTCGCGCG AGCGGTGGAC ACCCGGCTGG AAGAGCTTGG AGGGGAGCGG 
CTGCTGCAGC TGGGCCAGGG CGATGAGCTC TGCGGCCAGG AAGAGGCCTT CCGTGGTTGG 
GCAAAGGCGG CCTTCCAGGC CTCCTGTGAG ACCTTCTGCG TTGGGGAGGA GGCCAAGGCC 
GCCGCCCAGG ACATCTTCAG CCCCAAACGG AGCTGGAAAC GCCAGAGGTA CCGGCTGAGC 
ACCCAGGCCG AGGGCCTCCA GCTGCTGCCA GGCCTGATCC ACGTGCACAG ACGGAAGATG 
TTTCAGGCCA CAGTCCTCTC GGTGGAAAAT CTGCAAAGCA GCAAGTCCAC CCGGGCCACC 
ATCCTGGTGC GCCTGGACAC TGCAGGCCAG GAGGGGCTGC AGTACCAGCC GGGGGACCAC 
ATAGGCATCT GCCCGCCCAA CCGGCCGGGC CTGGTGGAGG CGCTGCTGAG CCGCGTGGAG 
GACCCGCCAC CGCCCACCGA GTCTGTGGCT GTGGAGCAGC TGGAGAAAGG CAGCCCAGGC 
GGCCCTCCTC CCAGCTGGGT GCGGGACCCA CGGCTGCCCC CGTGCACGCT GCGCCAGGCT 
CTCACCTTCT TCCTGGACAT CACCTCCCCA CCCAGCCCCC GGCTTCTCCG ACTGCTCAGC 
ACCCTGGCCG AAGAACCCAG CGAGCAGCAG GAGCTTGAGA CCCTCAGTCA GGACCCCCGG 
CGCTACGAGG AGTGGAAGTG GTTCCGCTGC CCCACGCTGC TGGAGGTGCT GGAGCAGTTC 
CCGTCCGTGG CGCTGCCCGC CCCGCTGCTC CTCACCCAGC TGCCCCTGCT GCAGCCCCGG 
TACTACTCTG TCAGCTCGGC CCCCAACGCC CACCCCGGAG AGGTCCACCT CACAGTGGCC 
GTGCTGGCGT ACAGGACCCA AGATGGGCTG GGCCCCCTAC ACTACGGGGT CTGCTCCACA 
TGGCTGAGCC AGCTCAAGAC TGGAGACCCC GTGCCCTGCT TCATCAGGGG GGCTCCCTCC 
TTCCGGCTGC CGCCTGACCC CTACGTGCCC TGCATCCTCG TGGGCCCTGG CACTGGCATC 
GCCCCCTTCC GGGGATTTTG GCAGGAGAGG CTGCATGACA TTGAGAGCAA AGGGCTGCAG 
CCCGCCCCCA TGACCCTGGT GTTCGGCTGC CGCTGCTCCC AACTCGACCA TCTCTACCGC 
GACGAGGTGC AGGACGCCCA GGAGCGCGGG GTGTTTGGCC GCGTCCTCAC CGCCTTCTCC 
CGGGAACCTG ACAGCCCCAA GACCTACGTA CAGGACATCC TGAGAACCGA GCTGGCTGCC 
GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTCTGCGG CGATGTCACT 
ATGGCAACCA GCGTCCTGCA GACGGTGCAG CGCATCTTGG CGACAGAGGG CGACATGGAG 
CTGGACGAGG CGGGCGACGT CATCGGCGTG CTGCGGGATC AGCAACGCTA TCACGAGGAC 
ATTTTCGGCC TCACGCTGCG CACCCAGGAG GTGACAAGCC GTATACGTAC CCAGAGCTTT 
TCCCTGCAGG AGCGGCATCT GCGGGGCGCG GTGCCCTGGG CCTTCGACCC GCCCGGCCCA 
GACACCCCCG GCCCCTGA