PRKAR2B cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKAR2B
Entrez Gene ID	282463
Full Name	protein kinase cAMP-dependent type II regulatory subunit beta
General protein information	Preferred Names protein kinase cAMP-dependent type II regulatory subunit beta Names cAMP-dependent protein kinase type II-beta regulatory subunit protein kinase, cAMP-dependent, regulatory, type II, beta
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174649.2	NP_777074.1	cAMP-dependent protein kinase type II-beta regulatory subunit

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Prkar2b	NP_035288.2
Bos taurus (cattle)	PRKAR2B	NP_777074.1
Rattus norvegicus (Norway rat)	Prkar2b	NP_001025191.1
Xenopus tropicalis (tropical clawed frog)	prkar2b	XP_002933139.2
Homo sapiens (human)	PRKAR2B	NP_002727.2
	PRKAR2B	XP_001089109.2
Gallus gallus (chicken)	PRKAR2B	XP_001232697.3
Pan troglodytes (chimpanzee)	PRKAR2B	XP_001148361.2
Danio rerio (zebrafish)	LOC100331387	XP_002666815.3
Canis lupus familiaris (dog)	PRKAR2B	XP_858066.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Conformational differences among solution structures of the type Ialpha, IIalpha and IIbeta protein kinase A regulatory subunit homodimers: role of the linker regions.
Vigil D, Blumenthal DK, Heller WT, Brown S, Canaves JM, Taylor SS, Trewhella J
Journal of molecular biology337(5)1183-94(2004 Apr)

Synteny mapping of five human chromosome 7 genes on bovine chromosomes 4 and 21.
Antoniou E, Womack JE, Grosz MD
Cytogenetics and cell genetics84(1-2)121-4(1999)

Identification of the MAP2- and P75-binding domain in the regulatory subunit (RII beta) of type II cAMP-dependent protein kinase. Cloning and expression of the cDNA for bovine brain RII beta.
Luo Z, Shafit-Zagardo B, Erlichman J
The Journal of biological chemistry265(35)21804-10(1990 Dec)

The RII subunit of cAMP-dependent protein kinase binds to a common amino-terminal domain in microtubule-associated proteins 2A, 2B, and 2C.
Obar RA, Dingus J, Bayley H, Vallee RB
Neuron3(5)639-45(1989 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

angle X-ray scattering studies indicate that the RIalpha, RIIalpha, and RIIbeta homodimers differ markedly in overall shape, despite extensive sequence homology and similar molecular masses
Title: Conformational differences among solution structures of the type Ialpha, IIalpha and IIbeta protein kinase A regulatory subunit homodimers: role of the linker regions.

The following PRKAR2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKAR2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa43639	NM_174649.2 Latest version!	Bos taurus protein kinase cAMP-dependent type II regulatory subunit beta (PRKAR2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKAR2B ( NM_174649.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777074.1 Bos taurus protein kinase cAMP-dependent type II regulatory subunit beta (PRKAR2B), mRNA.

Price & Availability↑

CloneID	OBa43639
Clone ID Related Accession (Same CDS sequence)	NM_174649.2
Accession Version	NM_174649.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Bos taurus(cattle)
Product	cAMP-dependent protein kinase type II-beta regulatory subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J05692.1. On Jun 3, 2003 this sequence version replaced NM_174649.1. ##Evidence-Data-START## Transcript exon combination :: J05692.1, BC151299.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGAGCATCG AGATCCCAGC GGGACTGACG GAGCTGCTGC AGGGCTTCAC CGTGGAGGTC 
CTGAGGCACC AGCCCGCGGA CCTGCTGGAG TTCGCGCTGC AGCACTTCAC ACGCCTGCAG 
GAGGAGAACG AGCGCAAAGG CACCGCGCGC TTCGGCCATG AAGGCAGGAC CTGGGGGGAC 
GCGGGCGCCG CCGGCGGGGG CGGCACCCCC AGCAAGGGGG TCAACTTCGC CGAGGAGCCC 
AGGCACAGCG ATTCGGAGAA CGGCGAGGAG GAGGAGGAAG AGGCCGCGGA TGCAGGGGCA 
TTCAACGCTC CAGTAATAAA CCGATTTACA AGGCGTGCCT CAGTATGTGC AGAAGCTTAT 
AATCCTGATG AAGAAGAAGA TGATGCTGAA TCCAGGATTA TACATCCCAA AACTGACGAT 
CAAAGAAATC GATTGCAAGA GGCTTGCAAA GACATCCTGC TATTTAAGAA CCTGGATCCG 
GAGCAGATGT CTCAAGTATT AGATGCCATG TTTGAAAAAT TGGTTAAAGA AGGGGAACAT 
GTAATTGATC AAGGTGATGA TGGCGACAAC TTTTATGTAA TTGATAGAGG AACGTTTGAT 
ATTTATGTGA AATGCGATGG TGTTGGACGG TGTGTTGGCA ACTATGACAA TCGTGGGAGT 
TTTGGCGAAC TGGCCTTAAT GTACAACACA CCCAGAGCAG CTACAATCAC TGCTACCTCT 
CCTGGTGCTC TGTGGGGTTT GGACAGGGTA ACCTTCAGGA GGATAATTGT AAAAAATAAT 
GCCAAAAAGA GAAAAATGTA TGAAAGCTTT ATTGAGTCAC TGCCATTCCT TAAATCTTTG 
GAAGTTTCTG AACGCCTGAA AGTGGTAGAC GTGATAGGCA CCAAAGTATA CAACGATGGA 
GAACAAATCA TTGCTCAGGG AGATTCGGCT GATTCTTTTT TCATTGTAGA ATCGGGAGAA 
GTGAAAATTA CTATGAAAAG GAAGGGTAAG TCAGAGGTGG AGGAGAACGG TGCAGTTGAA 
ATCGCTCGGT GTTCTCGAGG GCAGTACTTC GGCGAACTGG CTCTGGTAAC GAACAAGCCC 
CGAGCCGCTT CCGCACACGC CATCGGGACC GTCAAATGTT TAGCCATGGA TGTGCAAGCA 
TTTGAAAGGC TTTTGGGACC TTGCATGGAA ATTATGAAAA GGAACATCGC TACCTATGAA 
GAGCAATTAG TTGCCCTGTT TGGAACAAAC ATGGATATTG TTGAACCCAC TGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777074.1
CDS	1..1257
Translation	MSIEIPAGLTELLQGFTVEVLRHQPADLLEFALQHFTRLQEENERKGTARFGHEGRTWGDAGAAGGGGTPSKGVNFAEEPRHSDSENGEEEEEEAADAGAFNAPVINRFTRRASVCAEAYNPDEEEDDAESRIIHPKTDDQRNRLQEACKDILLFKNLDPEQMSQVLDAMFEKLVKEGEHVIDQGDDGDNFYVIDRGTFDIYVKCDGVGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEVSERLKVVDVIGTKVYNDGEQIIAQGDSADSFFIVESGEVKITMKRKGKSEVEENGAVEIARCSRGQYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVALFGTNMDIVEPTA

Target ORF information:

RefSeq Version	NM_174649.2
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase cAMP-dependent type II regulatory subunit beta (PRKAR2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174649.2

ORF Insert Sequence:

ATGAGCATCG AGATCCCAGC GGGACTGACG GAGCTGCTGC AGGGCTTCAC CGTGGAGGTC 
CTGAGGCACC AGCCCGCGGA CCTGCTGGAG TTCGCGCTGC AGCACTTCAC ACGCCTGCAG 
GAGGAGAACG AGCGCAAAGG CACCGCGCGC TTCGGCCATG AAGGCAGGAC CTGGGGGGAC 
GCGGGCGCCG CCGGCGGGGG CGGCACCCCC AGCAAGGGGG TCAACTTCGC CGAGGAGCCC 
AGGCACAGCG ATTCGGAGAA CGGCGAGGAG GAGGAGGAAG AGGCCGCGGA TGCAGGGGCA 
TTCAACGCTC CAGTAATAAA CCGATTTACA AGGCGTGCCT CAGTATGTGC AGAAGCTTAT 
AATCCTGATG AAGAAGAAGA TGATGCTGAA TCCAGGATTA TACATCCCAA AACTGACGAT 
CAAAGAAATC GATTGCAAGA GGCTTGCAAA GACATCCTGC TATTTAAGAA CCTGGATCCG 
GAGCAGATGT CTCAAGTATT AGATGCCATG TTTGAAAAAT TGGTTAAAGA AGGGGAACAT 
GTAATTGATC AAGGTGATGA TGGCGACAAC TTTTATGTAA TTGATAGAGG AACGTTTGAT 
ATTTATGTGA AATGCGATGG TGTTGGACGG TGTGTTGGCA ACTATGACAA TCGTGGGAGT 
TTTGGCGAAC TGGCCTTAAT GTACAACACA CCCAGAGCAG CTACAATCAC TGCTACCTCT 
CCTGGTGCTC TGTGGGGTTT GGACAGGGTA ACCTTCAGGA GGATAATTGT AAAAAATAAT 
GCCAAAAAGA GAAAAATGTA TGAAAGCTTT ATTGAGTCAC TGCCATTCCT TAAATCTTTG 
GAAGTTTCTG AACGCCTGAA AGTGGTAGAC GTGATAGGCA CCAAAGTATA CAACGATGGA 
GAACAAATCA TTGCTCAGGG AGATTCGGCT GATTCTTTTT TCATTGTAGA ATCGGGAGAA 
GTGAAAATTA CTATGAAAAG GAAGGGTAAG TCAGAGGTGG AGGAGAACGG TGCAGTTGAA 
ATCGCTCGGT GTTCTCGAGG GCAGTACTTC GGCGAACTGG CTCTGGTAAC GAACAAGCCC 
CGAGCCGCTT CCGCACACGC CATCGGGACC GTCAAATGTT TAGCCATGGA TGTGCAAGCA 
TTTGAAAGGC TTTTGGGACC TTGCATGGAA ATTATGAAAA GGAACATCGC TACCTATGAA 
GAGCAATTAG TTGCCCTGTT TGGAACAAAC ATGGATATTG TTGAACCCAC TGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Conformational differences among solution structures of the type Ialpha, IIalpha and IIbeta protein kinase A regulatory subunit homodimers: role of the linker regions.
Journal of molecular biology337(5)1183-94(2004 Apr)
Vigil D,Blumenthal DK,Heller WT,Brown S,Canaves JM,Taylor SS,Trewhella J

Synteny mapping of five human chromosome 7 genes on bovine chromosomes 4 and 21.
Cytogenetics and cell genetics84(1-2)121-4(1999)
Antoniou E,Womack JE,Grosz MD

Identification of the MAP2- and P75-binding domain in the regulatory subunit (RII beta) of type II cAMP-dependent protein kinase. Cloning and expression of the cDNA for bovine brain RII beta.
The Journal of biological chemistry265(35)21804-10(1990 Dec)
Luo Z,Shafit-Zagardo B,Erlichman J

The RII subunit of cAMP-dependent protein kinase binds to a common amino-terminal domain in microtubule-associated proteins 2A, 2B, and 2C.
Neuron3(5)639-45(1989 Nov)
Obar RA,Dingus J,Bayley H,Vallee RB