PHKA1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015470418.1	XP_015325904.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X4
XM_002700010.6	XP_002700056.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
XM_002700010.5	XP_002700056.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
XM_003588156.4	XP_003588204.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X5
XM_015470416.1	XP_015325902.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
XM_002700009.6	XP_002700055.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
XM_015470415.1	XP_015325901.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
XM_005227993.3	XP_005228050.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X4
XM_024988834.1	XP_024844602.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X5
XM_002700009.5	XP_002700055.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
XM_005227993.4	XP_005228050.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X4
XM_003588156.5	XP_003588204.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X6
XM_015470417.1	XP_015325903.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
XM_015470419.1	XP_015325905.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X5
XM_005227992.3	XP_005228049.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
XM_005227992.4	XP_005228049.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa238820	XM_024988834.1 Latest version!	Bos taurus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa35458	XM_015470415.1 Latest version!	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35458	XM_005227992.4 Latest version!	Bos taurus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35459	XM_002700009.6 Latest version!	Bos taurus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35459	XM_015470416.1 Latest version!	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35460	XM_015470417.1 Latest version!	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35460	XM_002700010.6 Latest version!	Bos taurus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35461	XM_005227993.4 Latest version!	Bos taurus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35461	XM_015470418.1 Latest version!	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35462	XM_015470419.1 Latest version!	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35462	XM_003588156.5 Latest version!	Bos taurus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa35460	XM_002700010.5	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35459	XM_002700009.5	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35461	XM_005227993.3	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35462	XM_003588156.4	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa35458	XM_005227992.3	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

PHKA1 ( XM_024988834.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024844602.1 Bos taurus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa238820
Clone ID Related Accession (Same CDS sequence)	XM_024988834.1
Accession Version	XM_024988834.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG ATGTCCATAT GTATCTTCCT 
ACAAAGATAT TTCAGGCTTC CCGGCCTTCA TTCAACTTAC TTGGTTCACC TCATCCCCAA 
CAAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CAAGTCTGGG 
GAAGTGGACT TCAAAGCACT GGTTTTACAG TTAAAAGAGA CCTCCAGCTT ACAAGAACAA 
GCTGATATCC TCTACATGCT ATATACTATG AAAGGACCTG ACTGGGACAC TGGATTGTAT 
GATGAAGAGA GCATCACAGT CAGAGAGCTG CTTACTGAGC TATATGGCAA AGTTGGAGAA 
ATTCGTCACT GGGGCCTGAT CAGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA 
CTCGATGAGG CCTGCACAGA CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCC 
CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTACT CACTCAACTG 
ATAGATGAAG CCAGTGAAGG GGATATGAGC ATTTCAATTC TTACACAGGA AATAATGGTA 
TATCTAGCCA TGTATATGCG AACTCAACCT GGCCTCTTTG CTGAAATGTT TCGACTTCGA 
ATTGGTCTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCCCTTCG ATGCTCAGCT 
GAGGAAGCCA CAGATGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAG CCTCTTGCAT 
CATATACTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCTAC TGATTCAAAT 
ATCAGCCCTG CTATTTCAAT TCGTGAGGTT GGTGCTGTTG GAGCAACCAA AACAGAACGA 
ACTGGGATCA TGCAGTTAAA AAGTGAGATA AAGCAGGTGG ACTTTCGTAG ACTGTCTATC 
TCAACTGAAA GTCAGCCACC TGGAACCTCT ATGAGTTCAA GTGGTGCGTC CTTCTCTAGA 
GCATTTGGTC AACAGACATC TAAAGATAGT CGTCAAGGTC AATGGCAACG CCGAAGAAGG 
CTGGATGGCG CATTGAACAG AGTCCCAATT GGATTTTATC AAAAAGTATG GAAAGTTTTA 
CAGAAGTGTC ACGGACTTTC TGTGGAAGGT TTTGTTCTTC CTTCTTCAAC TACTAGAGAG 
ATGACTCCAG GCGAGATTAA ATTCTCTGTT CATGTGGAGT CTGTCCTGAA TCGTGTACCT 
CAGCCAGAGT ACCGTCAGCT TCTGGTTGAA GCCATCCTTG TCCTCACCAT GATGGCAGAC 
ATTGAAATTC ATAGTATTGG AAGCATCATT GCTGTGGAGA AAATAGTGCA TATTGCCAAT 
GACTTGTTTC TTCAAGAACA GAAAACCCTC GGTGCAGATG ATATCATGTT GGCAAAGGAT 
CCTGCGTCTG GCATCTGTAC TCTTCTGTAT GACAGTGCAC CCAGTGGCAG GTTTGGCACC 
ATGACCTACC TCTCCAAAGC AGCGGCCACC TATGTGCAGG AGTTCCTGCC CCACAGCATC 
TGTGCCATGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024844602.1
CDS	112..3606
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSILPRASTSKEVDASLLSVISFPAFAVEDNQLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYREALEAVLIKGKNGVPLLPELYSVPPDKVDEEYKNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKSILKNKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNNMIVEMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDYDNELESGDWINGYGLSRNDVHMYLPTKIFQASRPSFNLLGSPHPQQEDQVPSVRVEIHLPRDKSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWDTGLYDEESITVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEVLTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATDGLMNLSPSAMKSLLHHILSGKEFGVERSVRPTDSNISPAISIREVGAVGATKTERTGIMQLKSEIKQVDFRRLSISTESQPPGTSMSSSGASFSRAFGQQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMMADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ

Target ORF information:

RefSeq Version	XM_024988834.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988834.1

ORF Insert Sequence:

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG ATGTCCATAT GTATCTTCCT 
ACAAAGATAT TTCAGGCTTC CCGGCCTTCA TTCAACTTAC TTGGTTCACC TCATCCCCAA 
CAAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CAAGTCTGGG 
GAAGTGGACT TCAAAGCACT GGTTTTACAG TTAAAAGAGA CCTCCAGCTT ACAAGAACAA 
GCTGATATCC TCTACATGCT ATATACTATG AAAGGACCTG ACTGGGACAC TGGATTGTAT 
GATGAAGAGA GCATCACAGT CAGAGAGCTG CTTACTGAGC TATATGGCAA AGTTGGAGAA 
ATTCGTCACT GGGGCCTGAT CAGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA 
CTCGATGAGG CCTGCACAGA CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCC 
CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTACT CACTCAACTG 
ATAGATGAAG CCAGTGAAGG GGATATGAGC ATTTCAATTC TTACACAGGA AATAATGGTA 
TATCTAGCCA TGTATATGCG AACTCAACCT GGCCTCTTTG CTGAAATGTT TCGACTTCGA 
ATTGGTCTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCCCTTCG ATGCTCAGCT 
GAGGAAGCCA CAGATGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAG CCTCTTGCAT 
CATATACTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCTAC TGATTCAAAT 
ATCAGCCCTG CTATTTCAAT TCGTGAGGTT GGTGCTGTTG GAGCAACCAA AACAGAACGA 
ACTGGGATCA TGCAGTTAAA AAGTGAGATA AAGCAGGTGG ACTTTCGTAG ACTGTCTATC 
TCAACTGAAA GTCAGCCACC TGGAACCTCT ATGAGTTCAA GTGGTGCGTC CTTCTCTAGA 
GCATTTGGTC AACAGACATC TAAAGATAGT CGTCAAGGTC AATGGCAACG CCGAAGAAGG 
CTGGATGGCG CATTGAACAG AGTCCCAATT GGATTTTATC AAAAAGTATG GAAAGTTTTA 
CAGAAGTGTC ACGGACTTTC TGTGGAAGGT TTTGTTCTTC CTTCTTCAAC TACTAGAGAG 
ATGACTCCAG GCGAGATTAA ATTCTCTGTT CATGTGGAGT CTGTCCTGAA TCGTGTACCT 
CAGCCAGAGT ACCGTCAGCT TCTGGTTGAA GCCATCCTTG TCCTCACCAT GATGGCAGAC 
ATTGAAATTC ATAGTATTGG AAGCATCATT GCTGTGGAGA AAATAGTGCA TATTGCCAAT 
GACTTGTTTC TTCAAGAACA GAAAACCCTC GGTGCAGATG ATATCATGTT GGCAAAGGAT 
CCTGCGTCTG GCATCTGTAC TCTTCTGTAT GACAGTGCAC CCAGTGGCAG GTTTGGCACC 
ATGACCTACC TCTCCAAAGC AGCGGCCACC TATGTGCAGG AGTTCCTGCC CCACAGCATC 
TGTGCCATGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_015470415.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325901.1 Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa35458
Clone ID Related Accession (Same CDS sequence)	XM_015470415.1 , XM_005227992.3 , XM_005227992.4
Accession Version	XM_015470415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647134.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG CTCACTCTGG TGATGAAGTT 
GCTCGTTATT TAGATCACCT TTTGGCACAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC 
TCACAGAAGG GAGGGCTAAA TCGGTTCCGA ACTGCTGTGC AAACAACCTG CGATTTAATG 
TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT GTACAGAATG TCCATATGTA TCTTCCTACA 
AAGATATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG GTTCACCTCA TCCCCAACAA 
GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACAA GTCTGGGGAA 
GTGGACTTCA AAGCACTGGT TTTACAGTTA AAAGAGACCT CCAGCTTACA AGAACAAGCT 
GATATCCTCT ACATGCTATA TACTATGAAA GGACCTGACT GGGACACTGG ATTGTATGAT 
GAAGAGAGCA TCACAGTCAG AGAGCTGCTT ACTGAGCTAT ATGGCAAAGT TGGAGAAATT 
CGTCACTGGG GCCTGATCAG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTC 
GATGAGGCCT GCACAGACCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCCCCA 
GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTACTCAC TCAACTGATA 
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATTCTTA CACAGGAAAT AATGGTATAT 
CTAGCCATGT ATATGCGAAC TCAACCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT 
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG 
GAAGCCACAG ATGGCCTGAT GAATCTCAGT CCTTCAGCCA TGAAGAGCCT CTTGCATCAT 
ATACTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCTACTGA TTCAAATATC 
AGCCCTGCTA TTTCAATTCG TGAGGTTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT 
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGGTGGACT TTCGTAGACT GTCTATCTCA 
ACTGAAAGTC AGGTGAAACA ACCACCTGGA ACCTCTATGA GTTCAAGTGG TGCGTCCTTC 
TCTAGAGCAT TTGGTCAACA GACATCTAAA GATAGTCGTC AAGGTCAATG GCAACGCCGA 
AGAAGGCTGG ATGGCGCATT GAACAGAGTC CCAATTGGAT TTTATCAAAA AGTATGGAAA 
GTTTTACAGA AGTGTCACGG ACTTTCTGTG GAAGGTTTTG TTCTTCCTTC TTCAACTACT 
AGAGAGATGA CTCCAGGCGA GATTAAATTC TCTGTTCATG TGGAGTCTGT CCTGAATCGT 
GTACCTCAGC CAGAGTACCG TCAGCTTCTG GTTGAAGCCA TCCTTGTCCT CACCATGATG 
GCAGACATTG AAATTCATAG TATTGGAAGC ATCATTGCTG TGGAGAAAAT AGTGCATATT 
GCCAATGACT TGTTTCTTCA AGAACAGAAA ACCCTCGGTG CAGATGATAT CATGTTGGCA 
AAGGATCCTG CGTCTGGCAT CTGTACTCTT CTGTATGACA GTGCACCCAG TGGCAGGTTT 
GGCACCATGA CCTACCTCTC CAAAGCAGCG GCCACCTATG TGCAGGAGTT CCTGCCCCAC 
AGCATCTGTG CCATGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325901.1
CDS	328..4008
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSILPRASTSKEVDASLLSVISFPAFAVEDNQLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYREALEAVLIKGKNGVPLLPELYSVPPDKVDEEYKNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKSILKNKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNNMIVEMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDYDNELESGDWINGYGLSRNAHSGDEVARYLDHLLAHTAPHPKLAPTSQKGGLNRFRTAVQTTCDLMSLVTKAKELHVQNVHMYLPTKIFQASRPSFNLLGSPHPQQEDQVPSVRVEIHLPRDKSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWDTGLYDEESITVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEVLTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATDGLMNLSPSAMKSLLHHILSGKEFGVERSVRPTDSNISPAISIREVGAVGATKTERTGIMQLKSEIKQVDFRRLSISTESQVKQPPGTSMSSSGASFSRAFGQQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMMADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ

Target ORF information:

RefSeq Version	XM_015470415.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470415.1

ORF Insert Sequence:

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG CTCACTCTGG TGATGAAGTT 
GCTCGTTATT TAGATCACCT TTTGGCACAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC 
TCACAGAAGG GAGGGCTAAA TCGGTTCCGA ACTGCTGTGC AAACAACCTG CGATTTAATG 
TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT GTACAGAATG TCCATATGTA TCTTCCTACA 
AAGATATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG GTTCACCTCA TCCCCAACAA 
GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACAA GTCTGGGGAA 
GTGGACTTCA AAGCACTGGT TTTACAGTTA AAAGAGACCT CCAGCTTACA AGAACAAGCT 
GATATCCTCT ACATGCTATA TACTATGAAA GGACCTGACT GGGACACTGG ATTGTATGAT 
GAAGAGAGCA TCACAGTCAG AGAGCTGCTT ACTGAGCTAT ATGGCAAAGT TGGAGAAATT 
CGTCACTGGG GCCTGATCAG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTC 
GATGAGGCCT GCACAGACCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCCCCA 
GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTACTCAC TCAACTGATA 
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATTCTTA CACAGGAAAT AATGGTATAT 
CTAGCCATGT ATATGCGAAC TCAACCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT 
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG 
GAAGCCACAG ATGGCCTGAT GAATCTCAGT CCTTCAGCCA TGAAGAGCCT CTTGCATCAT 
ATACTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCTACTGA TTCAAATATC 
AGCCCTGCTA TTTCAATTCG TGAGGTTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT 
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGGTGGACT TTCGTAGACT GTCTATCTCA 
ACTGAAAGTC AGGTGAAACA ACCACCTGGA ACCTCTATGA GTTCAAGTGG TGCGTCCTTC 
TCTAGAGCAT TTGGTCAACA GACATCTAAA GATAGTCGTC AAGGTCAATG GCAACGCCGA 
AGAAGGCTGG ATGGCGCATT GAACAGAGTC CCAATTGGAT TTTATCAAAA AGTATGGAAA 
GTTTTACAGA AGTGTCACGG ACTTTCTGTG GAAGGTTTTG TTCTTCCTTC TTCAACTACT 
AGAGAGATGA CTCCAGGCGA GATTAAATTC TCTGTTCATG TGGAGTCTGT CCTGAATCGT 
GTACCTCAGC CAGAGTACCG TCAGCTTCTG GTTGAAGCCA TCCTTGTCCT CACCATGATG 
GCAGACATTG AAATTCATAG TATTGGAAGC ATCATTGCTG TGGAGAAAAT AGTGCATATT 
GCCAATGACT TGTTTCTTCA AGAACAGAAA ACCCTCGGTG CAGATGATAT CATGTTGGCA 
AAGGATCCTG CGTCTGGCAT CTGTACTCTT CTGTATGACA GTGCACCCAG TGGCAGGTTT 
GGCACCATGA CCTACCTCTC CAAAGCAGCG GCCACCTATG TGCAGGAGTT CCTGCCCCAC 
AGCATCTGTG CCATGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_005227992.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228049.1 Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X1, mRNA.

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CloneID	OBa35458
Clone ID Related Accession (Same CDS sequence)	XM_015470415.1 , XM_005227992.3 , XM_005227992.4
Accession Version	XM_005227992.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104727.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227992.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG CTCACTCTGG TGATGAAGTT 
GCTCGTTATT TAGATCACCT TTTGGCACAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC 
TCACAGAAGG GAGGGCTAAA TCGGTTCCGA ACTGCTGTGC AAACAACCTG CGATTTAATG 
TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT GTACAGAATG TCCATATGTA TCTTCCTACA 
AAGATATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG GTTCACCTCA TCCCCAACAA 
GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACAA GTCTGGGGAA 
GTGGACTTCA AAGCACTGGT TTTACAGTTA AAAGAGACCT CCAGCTTACA AGAACAAGCT 
GATATCCTCT ACATGCTATA TACTATGAAA GGACCTGACT GGGACACTGG ATTGTATGAT 
GAAGAGAGCA TCACAGTCAG AGAGCTGCTT ACTGAGCTAT ATGGCAAAGT TGGAGAAATT 
CGTCACTGGG GCCTGATCAG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTC 
GATGAGGCCT GCACAGACCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCCCCA 
GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTACTCAC TCAACTGATA 
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATTCTTA CACAGGAAAT AATGGTATAT 
CTAGCCATGT ATATGCGAAC TCAACCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT 
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG 
GAAGCCACAG ATGGCCTGAT GAATCTCAGT CCTTCAGCCA TGAAGAGCCT CTTGCATCAT 
ATACTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCTACTGA TTCAAATATC 
AGCCCTGCTA TTTCAATTCG TGAGGTTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT 
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGGTGGACT TTCGTAGACT GTCTATCTCA 
ACTGAAAGTC AGGTGAAACA ACCACCTGGA ACCTCTATGA GTTCAAGTGG TGCGTCCTTC 
TCTAGAGCAT TTGGTCAACA GACATCTAAA GATAGTCGTC AAGGTCAATG GCAACGCCGA 
AGAAGGCTGG ATGGCGCATT GAACAGAGTC CCAATTGGAT TTTATCAAAA AGTATGGAAA 
GTTTTACAGA AGTGTCACGG ACTTTCTGTG GAAGGTTTTG TTCTTCCTTC TTCAACTACT 
AGAGAGATGA CTCCAGGCGA GATTAAATTC TCTGTTCATG TGGAGTCTGT CCTGAATCGT 
GTACCTCAGC CAGAGTACCG TCAGCTTCTG GTTGAAGCCA TCCTTGTCCT CACCATGATG 
GCAGACATTG AAATTCATAG TATTGGAAGC ATCATTGCTG TGGAGAAAAT AGTGCATATT 
GCCAATGACT TGTTTCTTCA AGAACAGAAA ACCCTCGGTG CAGATGATAT CATGTTGGCA 
AAGGATCCTG CGTCTGGCAT CTGTACTCTT CTGTATGACA GTGCACCCAG TGGCAGGTTT 
GGCACCATGA CCTACCTCTC CAAAGCAGCG GCCACCTATG TGCAGGAGTT CCTGCCCCAC 
AGCATCTGTG CCATGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228049.1
CDS	328..4008
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSILPRASTSKEVDASLLSVISFPAFAVEDNQLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYREALEAVLIKGKNGVPLLPELYSVPPDKVDEEYKNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKSILKNKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNNMIVEMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDYDNELESGDWINGYGLSRNAHSGDEVARYLDHLLAHTAPHPKLAPTSQKGGLNRFRTAVQTTCDLMSLVTKAKELHVQNVHMYLPTKIFQASRPSFNLLGSPHPQQEDQVPSVRVEIHLPRDKSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWDTGLYDEESITVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEVLTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATDGLMNLSPSAMKSLLHHILSGKEFGVERSVRPTDSNISPAISIREVGAVGATKTERTGIMQLKSEIKQVDFRRLSISTESQVKQPPGTSMSSSGASFSRAFGQQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMMADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ

Target ORF information:

RefSeq Version	XM_005227992.3
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227992.3

ORF Insert Sequence:

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG CTCACTCTGG TGATGAAGTT 
GCTCGTTATT TAGATCACCT TTTGGCACAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC 
TCACAGAAGG GAGGGCTAAA TCGGTTCCGA ACTGCTGTGC AAACAACCTG CGATTTAATG 
TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT GTACAGAATG TCCATATGTA TCTTCCTACA 
AAGATATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG GTTCACCTCA TCCCCAACAA 
GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACAA GTCTGGGGAA 
GTGGACTTCA AAGCACTGGT TTTACAGTTA AAAGAGACCT CCAGCTTACA AGAACAAGCT 
GATATCCTCT ACATGCTATA TACTATGAAA GGACCTGACT GGGACACTGG ATTGTATGAT 
GAAGAGAGCA TCACAGTCAG AGAGCTGCTT ACTGAGCTAT ATGGCAAAGT TGGAGAAATT 
CGTCACTGGG GCCTGATCAG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTC 
GATGAGGCCT GCACAGACCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCCCCA 
GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTACTCAC TCAACTGATA 
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATTCTTA CACAGGAAAT AATGGTATAT 
CTAGCCATGT ATATGCGAAC TCAACCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT 
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG 
GAAGCCACAG ATGGCCTGAT GAATCTCAGT CCTTCAGCCA TGAAGAGCCT CTTGCATCAT 
ATACTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCTACTGA TTCAAATATC 
AGCCCTGCTA TTTCAATTCG TGAGGTTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT 
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGGTGGACT TTCGTAGACT GTCTATCTCA 
ACTGAAAGTC AGGTGAAACA ACCACCTGGA ACCTCTATGA GTTCAAGTGG TGCGTCCTTC 
TCTAGAGCAT TTGGTCAACA GACATCTAAA GATAGTCGTC AAGGTCAATG GCAACGCCGA 
AGAAGGCTGG ATGGCGCATT GAACAGAGTC CCAATTGGAT TTTATCAAAA AGTATGGAAA 
GTTTTACAGA AGTGTCACGG ACTTTCTGTG GAAGGTTTTG TTCTTCCTTC TTCAACTACT 
AGAGAGATGA CTCCAGGCGA GATTAAATTC TCTGTTCATG TGGAGTCTGT CCTGAATCGT 
GTACCTCAGC CAGAGTACCG TCAGCTTCTG GTTGAAGCCA TCCTTGTCCT CACCATGATG 
GCAGACATTG AAATTCATAG TATTGGAAGC ATCATTGCTG TGGAGAAAAT AGTGCATATT 
GCCAATGACT TGTTTCTTCA AGAACAGAAA ACCCTCGGTG CAGATGATAT CATGTTGGCA 
AAGGATCCTG CGTCTGGCAT CTGTACTCTT CTGTATGACA GTGCACCCAG TGGCAGGTTT 
GGCACCATGA CCTACCTCTC CAAAGCAGCG GCCACCTATG TGCAGGAGTT CCTGCCCCAC 
AGCATCTGTG CCATGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_005227992.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228049.1 Bos taurus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa35458
Clone ID Related Accession (Same CDS sequence)	XM_015470415.1 , XM_005227992.3 , XM_005227992.4
Accession Version	XM_005227992.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005227992.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG CTCACTCTGG TGATGAAGTT 
GCTCGTTATT TAGATCACCT TTTGGCACAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC 
TCACAGAAGG GAGGGCTAAA TCGGTTCCGA ACTGCTGTGC AAACAACCTG CGATTTAATG 
TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT GTACAGAATG TCCATATGTA TCTTCCTACA 
AAGATATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG GTTCACCTCA TCCCCAACAA 
GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACAA GTCTGGGGAA 
GTGGACTTCA AAGCACTGGT TTTACAGTTA AAAGAGACCT CCAGCTTACA AGAACAAGCT 
GATATCCTCT ACATGCTATA TACTATGAAA GGACCTGACT GGGACACTGG ATTGTATGAT 
GAAGAGAGCA TCACAGTCAG AGAGCTGCTT ACTGAGCTAT ATGGCAAAGT TGGAGAAATT 
CGTCACTGGG GCCTGATCAG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTC 
GATGAGGCCT GCACAGACCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCCCCA 
GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTACTCAC TCAACTGATA 
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATTCTTA CACAGGAAAT AATGGTATAT 
CTAGCCATGT ATATGCGAAC TCAACCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT 
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG 
GAAGCCACAG ATGGCCTGAT GAATCTCAGT CCTTCAGCCA TGAAGAGCCT CTTGCATCAT 
ATACTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCTACTGA TTCAAATATC 
AGCCCTGCTA TTTCAATTCG TGAGGTTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT 
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGGTGGACT TTCGTAGACT GTCTATCTCA 
ACTGAAAGTC AGGTGAAACA ACCACCTGGA ACCTCTATGA GTTCAAGTGG TGCGTCCTTC 
TCTAGAGCAT TTGGTCAACA GACATCTAAA GATAGTCGTC AAGGTCAATG GCAACGCCGA 
AGAAGGCTGG ATGGCGCATT GAACAGAGTC CCAATTGGAT TTTATCAAAA AGTATGGAAA 
GTTTTACAGA AGTGTCACGG ACTTTCTGTG GAAGGTTTTG TTCTTCCTTC TTCAACTACT 
AGAGAGATGA CTCCAGGCGA GATTAAATTC TCTGTTCATG TGGAGTCTGT CCTGAATCGT 
GTACCTCAGC CAGAGTACCG TCAGCTTCTG GTTGAAGCCA TCCTTGTCCT CACCATGATG 
GCAGACATTG AAATTCATAG TATTGGAAGC ATCATTGCTG TGGAGAAAAT AGTGCATATT 
GCCAATGACT TGTTTCTTCA AGAACAGAAA ACCCTCGGTG CAGATGATAT CATGTTGGCA 
AAGGATCCTG CGTCTGGCAT CTGTACTCTT CTGTATGACA GTGCACCCAG TGGCAGGTTT 
GGCACCATGA CCTACCTCTC CAAAGCAGCG GCCACCTATG TGCAGGAGTT CCTGCCCCAC 
AGCATCTGTG CCATGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228049.1
CDS	346..4026
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSILPRASTSKEVDASLLSVISFPAFAVEDNQLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYREALEAVLIKGKNGVPLLPELYSVPPDKVDEEYKNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKSILKNKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNNMIVEMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDYDNELESGDWINGYGLSRNAHSGDEVARYLDHLLAHTAPHPKLAPTSQKGGLNRFRTAVQTTCDLMSLVTKAKELHVQNVHMYLPTKIFQASRPSFNLLGSPHPQQEDQVPSVRVEIHLPRDKSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWDTGLYDEESITVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEVLTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATDGLMNLSPSAMKSLLHHILSGKEFGVERSVRPTDSNISPAISIREVGAVGATKTERTGIMQLKSEIKQVDFRRLSISTESQVKQPPGTSMSSSGASFSRAFGQQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMMADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ

Target ORF information:

RefSeq Version	XM_005227992.4
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227992.4

ORF Insert Sequence:

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG CTCACTCTGG TGATGAAGTT 
GCTCGTTATT TAGATCACCT TTTGGCACAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC 
TCACAGAAGG GAGGGCTAAA TCGGTTCCGA ACTGCTGTGC AAACAACCTG CGATTTAATG 
TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT GTACAGAATG TCCATATGTA TCTTCCTACA 
AAGATATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG GTTCACCTCA TCCCCAACAA 
GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACAA GTCTGGGGAA 
GTGGACTTCA AAGCACTGGT TTTACAGTTA AAAGAGACCT CCAGCTTACA AGAACAAGCT 
GATATCCTCT ACATGCTATA TACTATGAAA GGACCTGACT GGGACACTGG ATTGTATGAT 
GAAGAGAGCA TCACAGTCAG AGAGCTGCTT ACTGAGCTAT ATGGCAAAGT TGGAGAAATT 
CGTCACTGGG GCCTGATCAG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTC 
GATGAGGCCT GCACAGACCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCCCCA 
GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTACTCAC TCAACTGATA 
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATTCTTA CACAGGAAAT AATGGTATAT 
CTAGCCATGT ATATGCGAAC TCAACCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT 
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG 
GAAGCCACAG ATGGCCTGAT GAATCTCAGT CCTTCAGCCA TGAAGAGCCT CTTGCATCAT 
ATACTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCTACTGA TTCAAATATC 
AGCCCTGCTA TTTCAATTCG TGAGGTTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT 
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGGTGGACT TTCGTAGACT GTCTATCTCA 
ACTGAAAGTC AGGTGAAACA ACCACCTGGA ACCTCTATGA GTTCAAGTGG TGCGTCCTTC 
TCTAGAGCAT TTGGTCAACA GACATCTAAA GATAGTCGTC AAGGTCAATG GCAACGCCGA 
AGAAGGCTGG ATGGCGCATT GAACAGAGTC CCAATTGGAT TTTATCAAAA AGTATGGAAA 
GTTTTACAGA AGTGTCACGG ACTTTCTGTG GAAGGTTTTG TTCTTCCTTC TTCAACTACT 
AGAGAGATGA CTCCAGGCGA GATTAAATTC TCTGTTCATG TGGAGTCTGT CCTGAATCGT 
GTACCTCAGC CAGAGTACCG TCAGCTTCTG GTTGAAGCCA TCCTTGTCCT CACCATGATG 
GCAGACATTG AAATTCATAG TATTGGAAGC ATCATTGCTG TGGAGAAAAT AGTGCATATT 
GCCAATGACT TGTTTCTTCA AGAACAGAAA ACCCTCGGTG CAGATGATAT CATGTTGGCA 
AAGGATCCTG CGTCTGGCAT CTGTACTCTT CTGTATGACA GTGCACCCAG TGGCAGGTTT 
GGCACCATGA CCTACCTCTC CAAAGCAGCG GCCACCTATG TGCAGGAGTT CCTGCCCCAC 
AGCATCTGTG CCATGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_015470416.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325902.1 Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa35459
Clone ID Related Accession (Same CDS sequence)	XM_015470416.1 , XM_002700009.6 , XM_002700009.5
Accession Version	XM_015470416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647134.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG CTCACTCTGG TGATGAAGTT 
GCTCGTTATT TAGATCACCT TTTGGCACAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC 
TCACAGAAGG GAGGGCTAAA TCGGTTCCGA ACTGCTGTGC AAACAACCTG CGATTTAATG 
TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT GTACAGAATG TCCATATGTA TCTTCCTACA 
AAGATATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG GTTCACCTCA TCCCCAACAA 
GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACAA GTCTGGGGAA 
GTGGACTTCA AAGCACTGGT TTTACAGTTA AAAGAGACCT CCAGCTTACA AGAACAAGCT 
GATATCCTCT ACATGCTATA TACTATGAAA GGACCTGACT GGGACACTGG ATTGTATGAT 
GAAGAGAGCA TCACAGTCAG AGAGCTGCTT ACTGAGCTAT ATGGCAAAGT TGGAGAAATT 
CGTCACTGGG GCCTGATCAG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTC 
GATGAGGCCT GCACAGACCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCCCCA 
GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTACTCAC TCAACTGATA 
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATTCTTA CACAGGAAAT AATGGTATAT 
CTAGCCATGT ATATGCGAAC TCAACCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT 
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG 
GAAGCCACAG ATGGCCTGAT GAATCTCAGT CCTTCAGCCA TGAAGAGCCT CTTGCATCAT 
ATACTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCTACTGA TTCAAATATC 
AGCCCTGCTA TTTCAATTCG TGAGGTTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT 
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGGTGGACT TTCGTAGACT GTCTATCTCA 
ACTGAAAGTC AGCCACCTGG AACCTCTATG AGTTCAAGTG GTGCGTCCTT CTCTAGAGCA 
TTTGGTCAAC AGACATCTAA AGATAGTCGT CAAGGTCAAT GGCAACGCCG AAGAAGGCTG 
GATGGCGCAT TGAACAGAGT CCCAATTGGA TTTTATCAAA AAGTATGGAA AGTTTTACAG 
AAGTGTCACG GACTTTCTGT GGAAGGTTTT GTTCTTCCTT CTTCAACTAC TAGAGAGATG 
ACTCCAGGCG AGATTAAATT CTCTGTTCAT GTGGAGTCTG TCCTGAATCG TGTACCTCAG 
CCAGAGTACC GTCAGCTTCT GGTTGAAGCC ATCCTTGTCC TCACCATGAT GGCAGACATT 
GAAATTCATA GTATTGGAAG CATCATTGCT GTGGAGAAAA TAGTGCATAT TGCCAATGAC 
TTGTTTCTTC AAGAACAGAA AACCCTCGGT GCAGATGATA TCATGTTGGC AAAGGATCCT 
GCGTCTGGCA TCTGTACTCT TCTGTATGAC AGTGCACCCA GTGGCAGGTT TGGCACCATG 
ACCTACCTCT CCAAAGCAGC GGCCACCTAT GTGCAGGAGT TCCTGCCCCA CAGCATCTGT 
GCCATGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325902.1
CDS	328..3999
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSILPRASTSKEVDASLLSVISFPAFAVEDNQLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYREALEAVLIKGKNGVPLLPELYSVPPDKVDEEYKNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKSILKNKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNNMIVEMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDYDNELESGDWINGYGLSRNAHSGDEVARYLDHLLAHTAPHPKLAPTSQKGGLNRFRTAVQTTCDLMSLVTKAKELHVQNVHMYLPTKIFQASRPSFNLLGSPHPQQEDQVPSVRVEIHLPRDKSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWDTGLYDEESITVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEVLTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATDGLMNLSPSAMKSLLHHILSGKEFGVERSVRPTDSNISPAISIREVGAVGATKTERTGIMQLKSEIKQVDFRRLSISTESQPPGTSMSSSGASFSRAFGQQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMMADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ

Target ORF information:

RefSeq Version	XM_015470416.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470416.1

ORF Insert Sequence:

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG CTCACTCTGG TGATGAAGTT 
GCTCGTTATT TAGATCACCT TTTGGCACAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC 
TCACAGAAGG GAGGGCTAAA TCGGTTCCGA ACTGCTGTGC AAACAACCTG CGATTTAATG 
TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT GTACAGAATG TCCATATGTA TCTTCCTACA 
AAGATATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG GTTCACCTCA TCCCCAACAA 
GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACAA GTCTGGGGAA 
GTGGACTTCA AAGCACTGGT TTTACAGTTA AAAGAGACCT CCAGCTTACA AGAACAAGCT 
GATATCCTCT ACATGCTATA TACTATGAAA GGACCTGACT GGGACACTGG ATTGTATGAT 
GAAGAGAGCA TCACAGTCAG AGAGCTGCTT ACTGAGCTAT ATGGCAAAGT TGGAGAAATT 
CGTCACTGGG GCCTGATCAG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTC 
GATGAGGCCT GCACAGACCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCCCCA 
GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTACTCAC TCAACTGATA 
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATTCTTA CACAGGAAAT AATGGTATAT 
CTAGCCATGT ATATGCGAAC TCAACCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT 
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG 
GAAGCCACAG ATGGCCTGAT GAATCTCAGT CCTTCAGCCA TGAAGAGCCT CTTGCATCAT 
ATACTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCTACTGA TTCAAATATC 
AGCCCTGCTA TTTCAATTCG TGAGGTTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT 
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGGTGGACT TTCGTAGACT GTCTATCTCA 
ACTGAAAGTC AGCCACCTGG AACCTCTATG AGTTCAAGTG GTGCGTCCTT CTCTAGAGCA 
TTTGGTCAAC AGACATCTAA AGATAGTCGT CAAGGTCAAT GGCAACGCCG AAGAAGGCTG 
GATGGCGCAT TGAACAGAGT CCCAATTGGA TTTTATCAAA AAGTATGGAA AGTTTTACAG 
AAGTGTCACG GACTTTCTGT GGAAGGTTTT GTTCTTCCTT CTTCAACTAC TAGAGAGATG 
ACTCCAGGCG AGATTAAATT CTCTGTTCAT GTGGAGTCTG TCCTGAATCG TGTACCTCAG 
CCAGAGTACC GTCAGCTTCT GGTTGAAGCC ATCCTTGTCC TCACCATGAT GGCAGACATT 
GAAATTCATA GTATTGGAAG CATCATTGCT GTGGAGAAAA TAGTGCATAT TGCCAATGAC 
TTGTTTCTTC AAGAACAGAA AACCCTCGGT GCAGATGATA TCATGTTGGC AAAGGATCCT 
GCGTCTGGCA TCTGTACTCT TCTGTATGAC AGTGCACCCA GTGGCAGGTT TGGCACCATG 
ACCTACCTCT CCAAAGCAGC GGCCACCTAT GTGCAGGAGT TCCTGCCCCA CAGCATCTGT 
GCCATGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_002700009.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002700055.1 Bos taurus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X2, mRNA.

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CloneID	OBa35459
Clone ID Related Accession (Same CDS sequence)	XM_015470416.1 , XM_002700009.6 , XM_002700009.5
Accession Version	XM_002700009.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002700009.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG CTCACTCTGG TGATGAAGTT 
GCTCGTTATT TAGATCACCT TTTGGCACAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC 
TCACAGAAGG GAGGGCTAAA TCGGTTCCGA ACTGCTGTGC AAACAACCTG CGATTTAATG 
TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT GTACAGAATG TCCATATGTA TCTTCCTACA 
AAGATATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG GTTCACCTCA TCCCCAACAA 
GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACAA GTCTGGGGAA 
GTGGACTTCA AAGCACTGGT TTTACAGTTA AAAGAGACCT CCAGCTTACA AGAACAAGCT 
GATATCCTCT ACATGCTATA TACTATGAAA GGACCTGACT GGGACACTGG ATTGTATGAT 
GAAGAGAGCA TCACAGTCAG AGAGCTGCTT ACTGAGCTAT ATGGCAAAGT TGGAGAAATT 
CGTCACTGGG GCCTGATCAG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTC 
GATGAGGCCT GCACAGACCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCCCCA 
GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTACTCAC TCAACTGATA 
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATTCTTA CACAGGAAAT AATGGTATAT 
CTAGCCATGT ATATGCGAAC TCAACCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT 
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG 
GAAGCCACAG ATGGCCTGAT GAATCTCAGT CCTTCAGCCA TGAAGAGCCT CTTGCATCAT 
ATACTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCTACTGA TTCAAATATC 
AGCCCTGCTA TTTCAATTCG TGAGGTTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT 
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGGTGGACT TTCGTAGACT GTCTATCTCA 
ACTGAAAGTC AGCCACCTGG AACCTCTATG AGTTCAAGTG GTGCGTCCTT CTCTAGAGCA 
TTTGGTCAAC AGACATCTAA AGATAGTCGT CAAGGTCAAT GGCAACGCCG AAGAAGGCTG 
GATGGCGCAT TGAACAGAGT CCCAATTGGA TTTTATCAAA AAGTATGGAA AGTTTTACAG 
AAGTGTCACG GACTTTCTGT GGAAGGTTTT GTTCTTCCTT CTTCAACTAC TAGAGAGATG 
ACTCCAGGCG AGATTAAATT CTCTGTTCAT GTGGAGTCTG TCCTGAATCG TGTACCTCAG 
CCAGAGTACC GTCAGCTTCT GGTTGAAGCC ATCCTTGTCC TCACCATGAT GGCAGACATT 
GAAATTCATA GTATTGGAAG CATCATTGCT GTGGAGAAAA TAGTGCATAT TGCCAATGAC 
TTGTTTCTTC AAGAACAGAA AACCCTCGGT GCAGATGATA TCATGTTGGC AAAGGATCCT 
GCGTCTGGCA TCTGTACTCT TCTGTATGAC AGTGCACCCA GTGGCAGGTT TGGCACCATG 
ACCTACCTCT CCAAAGCAGC GGCCACCTAT GTGCAGGAGT TCCTGCCCCA CAGCATCTGT 
GCCATGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002700055.1
CDS	280..3951
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSILPRASTSKEVDASLLSVISFPAFAVEDNQLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYREALEAVLIKGKNGVPLLPELYSVPPDKVDEEYKNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKSILKNKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNNMIVEMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDYDNELESGDWINGYGLSRNAHSGDEVARYLDHLLAHTAPHPKLAPTSQKGGLNRFRTAVQTTCDLMSLVTKAKELHVQNVHMYLPTKIFQASRPSFNLLGSPHPQQEDQVPSVRVEIHLPRDKSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWDTGLYDEESITVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEVLTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATDGLMNLSPSAMKSLLHHILSGKEFGVERSVRPTDSNISPAISIREVGAVGATKTERTGIMQLKSEIKQVDFRRLSISTESQPPGTSMSSSGASFSRAFGQQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMMADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ

Target ORF information:

RefSeq Version	XM_002700009.6
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002700009.6

ORF Insert Sequence:

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG CTCACTCTGG TGATGAAGTT 
GCTCGTTATT TAGATCACCT TTTGGCACAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC 
TCACAGAAGG GAGGGCTAAA TCGGTTCCGA ACTGCTGTGC AAACAACCTG CGATTTAATG 
TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT GTACAGAATG TCCATATGTA TCTTCCTACA 
AAGATATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG GTTCACCTCA TCCCCAACAA 
GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACAA GTCTGGGGAA 
GTGGACTTCA AAGCACTGGT TTTACAGTTA AAAGAGACCT CCAGCTTACA AGAACAAGCT 
GATATCCTCT ACATGCTATA TACTATGAAA GGACCTGACT GGGACACTGG ATTGTATGAT 
GAAGAGAGCA TCACAGTCAG AGAGCTGCTT ACTGAGCTAT ATGGCAAAGT TGGAGAAATT 
CGTCACTGGG GCCTGATCAG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTC 
GATGAGGCCT GCACAGACCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCCCCA 
GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTACTCAC TCAACTGATA 
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATTCTTA CACAGGAAAT AATGGTATAT 
CTAGCCATGT ATATGCGAAC TCAACCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT 
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG 
GAAGCCACAG ATGGCCTGAT GAATCTCAGT CCTTCAGCCA TGAAGAGCCT CTTGCATCAT 
ATACTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCTACTGA TTCAAATATC 
AGCCCTGCTA TTTCAATTCG TGAGGTTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT 
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGGTGGACT TTCGTAGACT GTCTATCTCA 
ACTGAAAGTC AGCCACCTGG AACCTCTATG AGTTCAAGTG GTGCGTCCTT CTCTAGAGCA 
TTTGGTCAAC AGACATCTAA AGATAGTCGT CAAGGTCAAT GGCAACGCCG AAGAAGGCTG 
GATGGCGCAT TGAACAGAGT CCCAATTGGA TTTTATCAAA AAGTATGGAA AGTTTTACAG 
AAGTGTCACG GACTTTCTGT GGAAGGTTTT GTTCTTCCTT CTTCAACTAC TAGAGAGATG 
ACTCCAGGCG AGATTAAATT CTCTGTTCAT GTGGAGTCTG TCCTGAATCG TGTACCTCAG 
CCAGAGTACC GTCAGCTTCT GGTTGAAGCC ATCCTTGTCC TCACCATGAT GGCAGACATT 
GAAATTCATA GTATTGGAAG CATCATTGCT GTGGAGAAAA TAGTGCATAT TGCCAATGAC 
TTGTTTCTTC AAGAACAGAA AACCCTCGGT GCAGATGATA TCATGTTGGC AAAGGATCCT 
GCGTCTGGCA TCTGTACTCT TCTGTATGAC AGTGCACCCA GTGGCAGGTT TGGCACCATG 
ACCTACCTCT CCAAAGCAGC GGCCACCTAT GTGCAGGAGT TCCTGCCCCA CAGCATCTGT 
GCCATGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_002700009.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002700055.1 Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X2, mRNA.

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CloneID	OBa35459
Clone ID Related Accession (Same CDS sequence)	XM_015470416.1 , XM_002700009.6 , XM_002700009.5
Accession Version	XM_002700009.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104727.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002700009.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG CTCACTCTGG TGATGAAGTT 
GCTCGTTATT TAGATCACCT TTTGGCACAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC 
TCACAGAAGG GAGGGCTAAA TCGGTTCCGA ACTGCTGTGC AAACAACCTG CGATTTAATG 
TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT GTACAGAATG TCCATATGTA TCTTCCTACA 
AAGATATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG GTTCACCTCA TCCCCAACAA 
GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACAA GTCTGGGGAA 
GTGGACTTCA AAGCACTGGT TTTACAGTTA AAAGAGACCT CCAGCTTACA AGAACAAGCT 
GATATCCTCT ACATGCTATA TACTATGAAA GGACCTGACT GGGACACTGG ATTGTATGAT 
GAAGAGAGCA TCACAGTCAG AGAGCTGCTT ACTGAGCTAT ATGGCAAAGT TGGAGAAATT 
CGTCACTGGG GCCTGATCAG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTC 
GATGAGGCCT GCACAGACCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCCCCA 
GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTACTCAC TCAACTGATA 
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATTCTTA CACAGGAAAT AATGGTATAT 
CTAGCCATGT ATATGCGAAC TCAACCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT 
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG 
GAAGCCACAG ATGGCCTGAT GAATCTCAGT CCTTCAGCCA TGAAGAGCCT CTTGCATCAT 
ATACTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCTACTGA TTCAAATATC 
AGCCCTGCTA TTTCAATTCG TGAGGTTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT 
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGGTGGACT TTCGTAGACT GTCTATCTCA 
ACTGAAAGTC AGCCACCTGG AACCTCTATG AGTTCAAGTG GTGCGTCCTT CTCTAGAGCA 
TTTGGTCAAC AGACATCTAA AGATAGTCGT CAAGGTCAAT GGCAACGCCG AAGAAGGCTG 
GATGGCGCAT TGAACAGAGT CCCAATTGGA TTTTATCAAA AAGTATGGAA AGTTTTACAG 
AAGTGTCACG GACTTTCTGT GGAAGGTTTT GTTCTTCCTT CTTCAACTAC TAGAGAGATG 
ACTCCAGGCG AGATTAAATT CTCTGTTCAT GTGGAGTCTG TCCTGAATCG TGTACCTCAG 
CCAGAGTACC GTCAGCTTCT GGTTGAAGCC ATCCTTGTCC TCACCATGAT GGCAGACATT 
GAAATTCATA GTATTGGAAG CATCATTGCT GTGGAGAAAA TAGTGCATAT TGCCAATGAC 
TTGTTTCTTC AAGAACAGAA AACCCTCGGT GCAGATGATA TCATGTTGGC AAAGGATCCT 
GCGTCTGGCA TCTGTACTCT TCTGTATGAC AGTGCACCCA GTGGCAGGTT TGGCACCATG 
ACCTACCTCT CCAAAGCAGC GGCCACCTAT GTGCAGGAGT TCCTGCCCCA CAGCATCTGT 
GCCATGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002700055.1
CDS	328..3999
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSILPRASTSKEVDASLLSVISFPAFAVEDNQLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYREALEAVLIKGKNGVPLLPELYSVPPDKVDEEYKNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKSILKNKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNNMIVEMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDYDNELESGDWINGYGLSRNAHSGDEVARYLDHLLAHTAPHPKLAPTSQKGGLNRFRTAVQTTCDLMSLVTKAKELHVQNVHMYLPTKIFQASRPSFNLLGSPHPQQEDQVPSVRVEIHLPRDKSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWDTGLYDEESITVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEVLTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATDGLMNLSPSAMKSLLHHILSGKEFGVERSVRPTDSNISPAISIREVGAVGATKTERTGIMQLKSEIKQVDFRRLSISTESQPPGTSMSSSGASFSRAFGQQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMMADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ

Target ORF information:

RefSeq Version	XM_002700009.5
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002700009.5

ORF Insert Sequence:

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG CTCACTCTGG TGATGAAGTT 
GCTCGTTATT TAGATCACCT TTTGGCACAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC 
TCACAGAAGG GAGGGCTAAA TCGGTTCCGA ACTGCTGTGC AAACAACCTG CGATTTAATG 
TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT GTACAGAATG TCCATATGTA TCTTCCTACA 
AAGATATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG GTTCACCTCA TCCCCAACAA 
GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACAA GTCTGGGGAA 
GTGGACTTCA AAGCACTGGT TTTACAGTTA AAAGAGACCT CCAGCTTACA AGAACAAGCT 
GATATCCTCT ACATGCTATA TACTATGAAA GGACCTGACT GGGACACTGG ATTGTATGAT 
GAAGAGAGCA TCACAGTCAG AGAGCTGCTT ACTGAGCTAT ATGGCAAAGT TGGAGAAATT 
CGTCACTGGG GCCTGATCAG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTC 
GATGAGGCCT GCACAGACCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCCCCA 
GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTACTCAC TCAACTGATA 
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATTCTTA CACAGGAAAT AATGGTATAT 
CTAGCCATGT ATATGCGAAC TCAACCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT 
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG 
GAAGCCACAG ATGGCCTGAT GAATCTCAGT CCTTCAGCCA TGAAGAGCCT CTTGCATCAT 
ATACTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCTACTGA TTCAAATATC 
AGCCCTGCTA TTTCAATTCG TGAGGTTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT 
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGGTGGACT TTCGTAGACT GTCTATCTCA 
ACTGAAAGTC AGCCACCTGG AACCTCTATG AGTTCAAGTG GTGCGTCCTT CTCTAGAGCA 
TTTGGTCAAC AGACATCTAA AGATAGTCGT CAAGGTCAAT GGCAACGCCG AAGAAGGCTG 
GATGGCGCAT TGAACAGAGT CCCAATTGGA TTTTATCAAA AAGTATGGAA AGTTTTACAG 
AAGTGTCACG GACTTTCTGT GGAAGGTTTT GTTCTTCCTT CTTCAACTAC TAGAGAGATG 
ACTCCAGGCG AGATTAAATT CTCTGTTCAT GTGGAGTCTG TCCTGAATCG TGTACCTCAG 
CCAGAGTACC GTCAGCTTCT GGTTGAAGCC ATCCTTGTCC TCACCATGAT GGCAGACATT 
GAAATTCATA GTATTGGAAG CATCATTGCT GTGGAGAAAA TAGTGCATAT TGCCAATGAC 
TTGTTTCTTC AAGAACAGAA AACCCTCGGT GCAGATGATA TCATGTTGGC AAAGGATCCT 
GCGTCTGGCA TCTGTACTCT TCTGTATGAC AGTGCACCCA GTGGCAGGTT TGGCACCATG 
ACCTACCTCT CCAAAGCAGC GGCCACCTAT GTGCAGGAGT TCCTGCCCCA CAGCATCTGT 
GCCATGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_002700010.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002700056.1 Bos taurus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa35460
Clone ID Related Accession (Same CDS sequence)	XM_002700010.6 , XM_002700010.5 , XM_015470417.1
Accession Version	XM_002700010.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002700010.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG CTCACTCTGG TGATGAAGTT 
GCTCGTTATT TAGATCACCT TTTGGCACAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC 
TCACAGAAGG GAGGGCTAAA TCGGTTCCGA ACTGCTGTGC AAACAACCTG CGATTTAATG 
TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT GTACAGAATG TCCATATGTA TCTTCCTACA 
AAGATATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG GTTCACCTCA TCCCCAACAA 
GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACAA GTCTGGGGAA 
GTGGACTTCA AAGCACTGGT TTTACAGTTA AAAGAGACCT CCAGCTTACA AGAACAAGCT 
GATATCCTCT ACATGCTATA TACTATGAAA GGACCTGACT GGGACACTGG ATTGTATGAT 
GAAGAGAGCA TCACAGTCAG AGAGCTGCTT ACTGAGCTAT ATGGCAAAGT TGGAGAAATT 
CGTCACTGGG GCCTGATCAG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTC 
GATGAGGCCT GCACAGACCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCCCCA 
GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTACTCAC TCAACTGATA 
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATTCTTA CACAGGAAAT AATGGTATAT 
CTAGCCATGT ATATGCGAAC TCAACCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT 
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG 
GAAGCCACAG ATGGCCTGAT GAATCTCAGT CCTTCAGCCA TGAAGAGCCT CTTGCATCAT 
ATACTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCTACTGA TTCAAATATC 
AGCCCTGCTA TTTCAATTCG TGAGGTTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT 
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGCCACCTG GAACCTCTAT GAGTTCAAGT 
GGTGCGTCCT TCTCTAGAGC ATTTGGTCAA CAGACATCTA AAGATAGTCG TCAAGGTCAA 
TGGCAACGCC GAAGAAGGCT GGATGGCGCA TTGAACAGAG TCCCAATTGG ATTTTATCAA 
AAAGTATGGA AAGTTTTACA GAAGTGTCAC GGACTTTCTG TGGAAGGTTT TGTTCTTCCT 
TCTTCAACTA CTAGAGAGAT GACTCCAGGC GAGATTAAAT TCTCTGTTCA TGTGGAGTCT 
GTCCTGAATC GTGTACCTCA GCCAGAGTAC CGTCAGCTTC TGGTTGAAGC CATCCTTGTC 
CTCACCATGA TGGCAGACAT TGAAATTCAT AGTATTGGAA GCATCATTGC TGTGGAGAAA 
ATAGTGCATA TTGCCAATGA CTTGTTTCTT CAAGAACAGA AAACCCTCGG TGCAGATGAT 
ATCATGTTGG CAAAGGATCC TGCGTCTGGC ATCTGTACTC TTCTGTATGA CAGTGCACCC 
AGTGGCAGGT TTGGCACCAT GACCTACCTC TCCAAAGCAG CGGCCACCTA TGTGCAGGAG 
TTCCTGCCCC ACAGCATCTG TGCCATGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002700056.1
CDS	337..3969
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSILPRASTSKEVDASLLSVISFPAFAVEDNQLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYREALEAVLIKGKNGVPLLPELYSVPPDKVDEEYKNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKSILKNKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNNMIVEMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDYDNELESGDWINGYGLSRNAHSGDEVARYLDHLLAHTAPHPKLAPTSQKGGLNRFRTAVQTTCDLMSLVTKAKELHVQNVHMYLPTKIFQASRPSFNLLGSPHPQQEDQVPSVRVEIHLPRDKSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWDTGLYDEESITVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEVLTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATDGLMNLSPSAMKSLLHHILSGKEFGVERSVRPTDSNISPAISIREVGAVGATKTERTGIMQLKSEIKQPPGTSMSSSGASFSRAFGQQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMMADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ

Target ORF information:

RefSeq Version	XM_002700010.6
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002700010.6

ORF Insert Sequence:

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG CTCACTCTGG TGATGAAGTT 
GCTCGTTATT TAGATCACCT TTTGGCACAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC 
TCACAGAAGG GAGGGCTAAA TCGGTTCCGA ACTGCTGTGC AAACAACCTG CGATTTAATG 
TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT GTACAGAATG TCCATATGTA TCTTCCTACA 
AAGATATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG GTTCACCTCA TCCCCAACAA 
GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACAA GTCTGGGGAA 
GTGGACTTCA AAGCACTGGT TTTACAGTTA AAAGAGACCT CCAGCTTACA AGAACAAGCT 
GATATCCTCT ACATGCTATA TACTATGAAA GGACCTGACT GGGACACTGG ATTGTATGAT 
GAAGAGAGCA TCACAGTCAG AGAGCTGCTT ACTGAGCTAT ATGGCAAAGT TGGAGAAATT 
CGTCACTGGG GCCTGATCAG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTC 
GATGAGGCCT GCACAGACCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCCCCA 
GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTACTCAC TCAACTGATA 
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATTCTTA CACAGGAAAT AATGGTATAT 
CTAGCCATGT ATATGCGAAC TCAACCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT 
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG 
GAAGCCACAG ATGGCCTGAT GAATCTCAGT CCTTCAGCCA TGAAGAGCCT CTTGCATCAT 
ATACTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCTACTGA TTCAAATATC 
AGCCCTGCTA TTTCAATTCG TGAGGTTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT 
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGCCACCTG GAACCTCTAT GAGTTCAAGT 
GGTGCGTCCT TCTCTAGAGC ATTTGGTCAA CAGACATCTA AAGATAGTCG TCAAGGTCAA 
TGGCAACGCC GAAGAAGGCT GGATGGCGCA TTGAACAGAG TCCCAATTGG ATTTTATCAA 
AAAGTATGGA AAGTTTTACA GAAGTGTCAC GGACTTTCTG TGGAAGGTTT TGTTCTTCCT 
TCTTCAACTA CTAGAGAGAT GACTCCAGGC GAGATTAAAT TCTCTGTTCA TGTGGAGTCT 
GTCCTGAATC GTGTACCTCA GCCAGAGTAC CGTCAGCTTC TGGTTGAAGC CATCCTTGTC 
CTCACCATGA TGGCAGACAT TGAAATTCAT AGTATTGGAA GCATCATTGC TGTGGAGAAA 
ATAGTGCATA TTGCCAATGA CTTGTTTCTT CAAGAACAGA AAACCCTCGG TGCAGATGAT 
ATCATGTTGG CAAAGGATCC TGCGTCTGGC ATCTGTACTC TTCTGTATGA CAGTGCACCC 
AGTGGCAGGT TTGGCACCAT GACCTACCTC TCCAAAGCAG CGGCCACCTA TGTGCAGGAG 
TTCCTGCCCC ACAGCATCTG TGCCATGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_002700010.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002700056.1 Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X3, mRNA.

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CloneID	OBa35460
Clone ID Related Accession (Same CDS sequence)	XM_002700010.6 , XM_002700010.5 , XM_015470417.1
Accession Version	XM_002700010.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104727.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002700010.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG CTCACTCTGG TGATGAAGTT 
GCTCGTTATT TAGATCACCT TTTGGCACAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC 
TCACAGAAGG GAGGGCTAAA TCGGTTCCGA ACTGCTGTGC AAACAACCTG CGATTTAATG 
TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT GTACAGAATG TCCATATGTA TCTTCCTACA 
AAGATATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG GTTCACCTCA TCCCCAACAA 
GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACAA GTCTGGGGAA 
GTGGACTTCA AAGCACTGGT TTTACAGTTA AAAGAGACCT CCAGCTTACA AGAACAAGCT 
GATATCCTCT ACATGCTATA TACTATGAAA GGACCTGACT GGGACACTGG ATTGTATGAT 
GAAGAGAGCA TCACAGTCAG AGAGCTGCTT ACTGAGCTAT ATGGCAAAGT TGGAGAAATT 
CGTCACTGGG GCCTGATCAG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTC 
GATGAGGCCT GCACAGACCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCCCCA 
GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTACTCAC TCAACTGATA 
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATTCTTA CACAGGAAAT AATGGTATAT 
CTAGCCATGT ATATGCGAAC TCAACCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT 
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG 
GAAGCCACAG ATGGCCTGAT GAATCTCAGT CCTTCAGCCA TGAAGAGCCT CTTGCATCAT 
ATACTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCTACTGA TTCAAATATC 
AGCCCTGCTA TTTCAATTCG TGAGGTTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT 
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGCCACCTG GAACCTCTAT GAGTTCAAGT 
GGTGCGTCCT TCTCTAGAGC ATTTGGTCAA CAGACATCTA AAGATAGTCG TCAAGGTCAA 
TGGCAACGCC GAAGAAGGCT GGATGGCGCA TTGAACAGAG TCCCAATTGG ATTTTATCAA 
AAAGTATGGA AAGTTTTACA GAAGTGTCAC GGACTTTCTG TGGAAGGTTT TGTTCTTCCT 
TCTTCAACTA CTAGAGAGAT GACTCCAGGC GAGATTAAAT TCTCTGTTCA TGTGGAGTCT 
GTCCTGAATC GTGTACCTCA GCCAGAGTAC CGTCAGCTTC TGGTTGAAGC CATCCTTGTC 
CTCACCATGA TGGCAGACAT TGAAATTCAT AGTATTGGAA GCATCATTGC TGTGGAGAAA 
ATAGTGCATA TTGCCAATGA CTTGTTTCTT CAAGAACAGA AAACCCTCGG TGCAGATGAT 
ATCATGTTGG CAAAGGATCC TGCGTCTGGC ATCTGTACTC TTCTGTATGA CAGTGCACCC 
AGTGGCAGGT TTGGCACCAT GACCTACCTC TCCAAAGCAG CGGCCACCTA TGTGCAGGAG 
TTCCTGCCCC ACAGCATCTG TGCCATGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002700056.1
CDS	328..3960
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSILPRASTSKEVDASLLSVISFPAFAVEDNQLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYREALEAVLIKGKNGVPLLPELYSVPPDKVDEEYKNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKSILKNKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNNMIVEMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDYDNELESGDWINGYGLSRNAHSGDEVARYLDHLLAHTAPHPKLAPTSQKGGLNRFRTAVQTTCDLMSLVTKAKELHVQNVHMYLPTKIFQASRPSFNLLGSPHPQQEDQVPSVRVEIHLPRDKSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWDTGLYDEESITVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEVLTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATDGLMNLSPSAMKSLLHHILSGKEFGVERSVRPTDSNISPAISIREVGAVGATKTERTGIMQLKSEIKQPPGTSMSSSGASFSRAFGQQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMMADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ

Target ORF information:

RefSeq Version	XM_002700010.5
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002700010.5

ORF Insert Sequence:

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG CTCACTCTGG TGATGAAGTT 
GCTCGTTATT TAGATCACCT TTTGGCACAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC 
TCACAGAAGG GAGGGCTAAA TCGGTTCCGA ACTGCTGTGC AAACAACCTG CGATTTAATG 
TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT GTACAGAATG TCCATATGTA TCTTCCTACA 
AAGATATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG GTTCACCTCA TCCCCAACAA 
GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACAA GTCTGGGGAA 
GTGGACTTCA AAGCACTGGT TTTACAGTTA AAAGAGACCT CCAGCTTACA AGAACAAGCT 
GATATCCTCT ACATGCTATA TACTATGAAA GGACCTGACT GGGACACTGG ATTGTATGAT 
GAAGAGAGCA TCACAGTCAG AGAGCTGCTT ACTGAGCTAT ATGGCAAAGT TGGAGAAATT 
CGTCACTGGG GCCTGATCAG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTC 
GATGAGGCCT GCACAGACCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCCCCA 
GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTACTCAC TCAACTGATA 
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATTCTTA CACAGGAAAT AATGGTATAT 
CTAGCCATGT ATATGCGAAC TCAACCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT 
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG 
GAAGCCACAG ATGGCCTGAT GAATCTCAGT CCTTCAGCCA TGAAGAGCCT CTTGCATCAT 
ATACTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCTACTGA TTCAAATATC 
AGCCCTGCTA TTTCAATTCG TGAGGTTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT 
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGCCACCTG GAACCTCTAT GAGTTCAAGT 
GGTGCGTCCT TCTCTAGAGC ATTTGGTCAA CAGACATCTA AAGATAGTCG TCAAGGTCAA 
TGGCAACGCC GAAGAAGGCT GGATGGCGCA TTGAACAGAG TCCCAATTGG ATTTTATCAA 
AAAGTATGGA AAGTTTTACA GAAGTGTCAC GGACTTTCTG TGGAAGGTTT TGTTCTTCCT 
TCTTCAACTA CTAGAGAGAT GACTCCAGGC GAGATTAAAT TCTCTGTTCA TGTGGAGTCT 
GTCCTGAATC GTGTACCTCA GCCAGAGTAC CGTCAGCTTC TGGTTGAAGC CATCCTTGTC 
CTCACCATGA TGGCAGACAT TGAAATTCAT AGTATTGGAA GCATCATTGC TGTGGAGAAA 
ATAGTGCATA TTGCCAATGA CTTGTTTCTT CAAGAACAGA AAACCCTCGG TGCAGATGAT 
ATCATGTTGG CAAAGGATCC TGCGTCTGGC ATCTGTACTC TTCTGTATGA CAGTGCACCC 
AGTGGCAGGT TTGGCACCAT GACCTACCTC TCCAAAGCAG CGGCCACCTA TGTGCAGGAG 
TTCCTGCCCC ACAGCATCTG TGCCATGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_015470417.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325903.1 Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa35460
Clone ID Related Accession (Same CDS sequence)	XM_002700010.6 , XM_002700010.5 , XM_015470417.1
Accession Version	XM_015470417.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647134.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG CTCACTCTGG TGATGAAGTT 
GCTCGTTATT TAGATCACCT TTTGGCACAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC 
TCACAGAAGG GAGGGCTAAA TCGGTTCCGA ACTGCTGTGC AAACAACCTG CGATTTAATG 
TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT GTACAGAATG TCCATATGTA TCTTCCTACA 
AAGATATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG GTTCACCTCA TCCCCAACAA 
GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACAA GTCTGGGGAA 
GTGGACTTCA AAGCACTGGT TTTACAGTTA AAAGAGACCT CCAGCTTACA AGAACAAGCT 
GATATCCTCT ACATGCTATA TACTATGAAA GGACCTGACT GGGACACTGG ATTGTATGAT 
GAAGAGAGCA TCACAGTCAG AGAGCTGCTT ACTGAGCTAT ATGGCAAAGT TGGAGAAATT 
CGTCACTGGG GCCTGATCAG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTC 
GATGAGGCCT GCACAGACCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCCCCA 
GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTACTCAC TCAACTGATA 
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATTCTTA CACAGGAAAT AATGGTATAT 
CTAGCCATGT ATATGCGAAC TCAACCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT 
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG 
GAAGCCACAG ATGGCCTGAT GAATCTCAGT CCTTCAGCCA TGAAGAGCCT CTTGCATCAT 
ATACTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCTACTGA TTCAAATATC 
AGCCCTGCTA TTTCAATTCG TGAGGTTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT 
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGCCACCTG GAACCTCTAT GAGTTCAAGT 
GGTGCGTCCT TCTCTAGAGC ATTTGGTCAA CAGACATCTA AAGATAGTCG TCAAGGTCAA 
TGGCAACGCC GAAGAAGGCT GGATGGCGCA TTGAACAGAG TCCCAATTGG ATTTTATCAA 
AAAGTATGGA AAGTTTTACA GAAGTGTCAC GGACTTTCTG TGGAAGGTTT TGTTCTTCCT 
TCTTCAACTA CTAGAGAGAT GACTCCAGGC GAGATTAAAT TCTCTGTTCA TGTGGAGTCT 
GTCCTGAATC GTGTACCTCA GCCAGAGTAC CGTCAGCTTC TGGTTGAAGC CATCCTTGTC 
CTCACCATGA TGGCAGACAT TGAAATTCAT AGTATTGGAA GCATCATTGC TGTGGAGAAA 
ATAGTGCATA TTGCCAATGA CTTGTTTCTT CAAGAACAGA AAACCCTCGG TGCAGATGAT 
ATCATGTTGG CAAAGGATCC TGCGTCTGGC ATCTGTACTC TTCTGTATGA CAGTGCACCC 
AGTGGCAGGT TTGGCACCAT GACCTACCTC TCCAAAGCAG CGGCCACCTA TGTGCAGGAG 
TTCCTGCCCC ACAGCATCTG TGCCATGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325903.1
CDS	328..3960
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSILPRASTSKEVDASLLSVISFPAFAVEDNQLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYREALEAVLIKGKNGVPLLPELYSVPPDKVDEEYKNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKSILKNKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNNMIVEMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDYDNELESGDWINGYGLSRNAHSGDEVARYLDHLLAHTAPHPKLAPTSQKGGLNRFRTAVQTTCDLMSLVTKAKELHVQNVHMYLPTKIFQASRPSFNLLGSPHPQQEDQVPSVRVEIHLPRDKSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWDTGLYDEESITVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEVLTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATDGLMNLSPSAMKSLLHHILSGKEFGVERSVRPTDSNISPAISIREVGAVGATKTERTGIMQLKSEIKQPPGTSMSSSGASFSRAFGQQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMMADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ

Target ORF information:

RefSeq Version	XM_015470417.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470417.1

ORF Insert Sequence:

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG CTCACTCTGG TGATGAAGTT 
GCTCGTTATT TAGATCACCT TTTGGCACAC ACTGCTCCCC ATCCTAAACT AGCCCCTACC 
TCACAGAAGG GAGGGCTAAA TCGGTTCCGA ACTGCTGTGC AAACAACCTG CGATTTAATG 
TCCTTGGTGA CCAAGGCCAA GGAGCTGCAT GTACAGAATG TCCATATGTA TCTTCCTACA 
AAGATATTTC AGGCTTCCCG GCCTTCATTC AACTTACTTG GTTCACCTCA TCCCCAACAA 
GAGGACCAGG TTCCCTCTGT TCGTGTAGAA ATACATCTTC CTAGAGACAA GTCTGGGGAA 
GTGGACTTCA AAGCACTGGT TTTACAGTTA AAAGAGACCT CCAGCTTACA AGAACAAGCT 
GATATCCTCT ACATGCTATA TACTATGAAA GGACCTGACT GGGACACTGG ATTGTATGAT 
GAAGAGAGCA TCACAGTCAG AGAGCTGCTT ACTGAGCTAT ATGGCAAAGT TGGAGAAATT 
CGTCACTGGG GCCTGATCAG ATACATCTCT GGAATCTTAA GGAAGAAGGT GGAAGCACTC 
GATGAGGCCT GCACAGACCT TCTCTCCCAT CAGAAACATT TGACAGTGGG ACTCCCCCCA 
GAACCTCGAG AGAAGACCAT CTCTGCACCT CTGCCCTATG AAGTACTCAC TCAACTGATA 
GATGAAGCCA GTGAAGGGGA TATGAGCATT TCAATTCTTA CACAGGAAAT AATGGTATAT 
CTAGCCATGT ATATGCGAAC TCAACCTGGC CTCTTTGCTG AAATGTTTCG ACTTCGAATT 
GGTCTGATCA TACAAGTTAT GGCAACAGAA CTGGCCCACT CCCTTCGATG CTCAGCTGAG 
GAAGCCACAG ATGGCCTGAT GAATCTCAGT CCTTCAGCCA TGAAGAGCCT CTTGCATCAT 
ATACTCAGTG GCAAGGAGTT TGGAGTGGAA CGAAGTGTTC GTCCTACTGA TTCAAATATC 
AGCCCTGCTA TTTCAATTCG TGAGGTTGGT GCTGTTGGAG CAACCAAAAC AGAACGAACT 
GGGATCATGC AGTTAAAAAG TGAGATAAAG CAGCCACCTG GAACCTCTAT GAGTTCAAGT 
GGTGCGTCCT TCTCTAGAGC ATTTGGTCAA CAGACATCTA AAGATAGTCG TCAAGGTCAA 
TGGCAACGCC GAAGAAGGCT GGATGGCGCA TTGAACAGAG TCCCAATTGG ATTTTATCAA 
AAAGTATGGA AAGTTTTACA GAAGTGTCAC GGACTTTCTG TGGAAGGTTT TGTTCTTCCT 
TCTTCAACTA CTAGAGAGAT GACTCCAGGC GAGATTAAAT TCTCTGTTCA TGTGGAGTCT 
GTCCTGAATC GTGTACCTCA GCCAGAGTAC CGTCAGCTTC TGGTTGAAGC CATCCTTGTC 
CTCACCATGA TGGCAGACAT TGAAATTCAT AGTATTGGAA GCATCATTGC TGTGGAGAAA 
ATAGTGCATA TTGCCAATGA CTTGTTTCTT CAAGAACAGA AAACCCTCGG TGCAGATGAT 
ATCATGTTGG CAAAGGATCC TGCGTCTGGC ATCTGTACTC TTCTGTATGA CAGTGCACCC 
AGTGGCAGGT TTGGCACCAT GACCTACCTC TCCAAAGCAG CGGCCACCTA TGTGCAGGAG 
TTCCTGCCCC ACAGCATCTG TGCCATGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_015470418.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325904.1 Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X4, mRNA.

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CloneID	OBa35461
Clone ID Related Accession (Same CDS sequence)	XM_015470418.1 , XM_005227993.3 , XM_005227993.4
Accession Version	XM_015470418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647134.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG ATGTCCATAT GTATCTTCCT 
ACAAAGATAT TTCAGGCTTC CCGGCCTTCA TTCAACTTAC TTGGTTCACC TCATCCCCAA 
CAAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CAAGTCTGGG 
GAAGTGGACT TCAAAGCACT GGTTTTACAG TTAAAAGAGA CCTCCAGCTT ACAAGAACAA 
GCTGATATCC TCTACATGCT ATATACTATG AAAGGACCTG ACTGGGACAC TGGATTGTAT 
GATGAAGAGA GCATCACAGT CAGAGAGCTG CTTACTGAGC TATATGGCAA AGTTGGAGAA 
ATTCGTCACT GGGGCCTGAT CAGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA 
CTCGATGAGG CCTGCACAGA CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCC 
CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTACT CACTCAACTG 
ATAGATGAAG CCAGTGAAGG GGATATGAGC ATTTCAATTC TTACACAGGA AATAATGGTA 
TATCTAGCCA TGTATATGCG AACTCAACCT GGCCTCTTTG CTGAAATGTT TCGACTTCGA 
ATTGGTCTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCCCTTCG ATGCTCAGCT 
GAGGAAGCCA CAGATGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAG CCTCTTGCAT 
CATATACTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCTAC TGATTCAAAT 
ATCAGCCCTG CTATTTCAAT TCGTGAGGTT GGTGCTGTTG GAGCAACCAA AACAGAACGA 
ACTGGGATCA TGCAGTTAAA AAGTGAGATA AAGCAGGTGG ACTTTCGTAG ACTGTCTATC 
TCAACTGAAA GTCAGGTGAA ACAACCACCT GGAACCTCTA TGAGTTCAAG TGGTGCGTCC 
TTCTCTAGAG CATTTGGTCA ACAGACATCT AAAGATAGTC GTCAAGGTCA ATGGCAACGC 
CGAAGAAGGC TGGATGGCGC ATTGAACAGA GTCCCAATTG GATTTTATCA AAAAGTATGG 
AAAGTTTTAC AGAAGTGTCA CGGACTTTCT GTGGAAGGTT TTGTTCTTCC TTCTTCAACT 
ACTAGAGAGA TGACTCCAGG CGAGATTAAA TTCTCTGTTC ATGTGGAGTC TGTCCTGAAT 
CGTGTACCTC AGCCAGAGTA CCGTCAGCTT CTGGTTGAAG CCATCCTTGT CCTCACCATG 
ATGGCAGACA TTGAAATTCA TAGTATTGGA AGCATCATTG CTGTGGAGAA AATAGTGCAT 
ATTGCCAATG ACTTGTTTCT TCAAGAACAG AAAACCCTCG GTGCAGATGA TATCATGTTG 
GCAAAGGATC CTGCGTCTGG CATCTGTACT CTTCTGTATG ACAGTGCACC CAGTGGCAGG 
TTTGGCACCA TGACCTACCT CTCCAAAGCA GCGGCCACCT ATGTGCAGGA GTTCCTGCCC 
CACAGCATCT GTGCCATGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325904.1
CDS	328..3831
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSILPRASTSKEVDASLLSVISFPAFAVEDNQLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYREALEAVLIKGKNGVPLLPELYSVPPDKVDEEYKNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKSILKNKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNNMIVEMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDYDNELESGDWINGYGLSRNDVHMYLPTKIFQASRPSFNLLGSPHPQQEDQVPSVRVEIHLPRDKSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWDTGLYDEESITVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEVLTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATDGLMNLSPSAMKSLLHHILSGKEFGVERSVRPTDSNISPAISIREVGAVGATKTERTGIMQLKSEIKQVDFRRLSISTESQVKQPPGTSMSSSGASFSRAFGQQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMMADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ

Target ORF information:

RefSeq Version	XM_015470418.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470418.1

ORF Insert Sequence:

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG ATGTCCATAT GTATCTTCCT 
ACAAAGATAT TTCAGGCTTC CCGGCCTTCA TTCAACTTAC TTGGTTCACC TCATCCCCAA 
CAAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CAAGTCTGGG 
GAAGTGGACT TCAAAGCACT GGTTTTACAG TTAAAAGAGA CCTCCAGCTT ACAAGAACAA 
GCTGATATCC TCTACATGCT ATATACTATG AAAGGACCTG ACTGGGACAC TGGATTGTAT 
GATGAAGAGA GCATCACAGT CAGAGAGCTG CTTACTGAGC TATATGGCAA AGTTGGAGAA 
ATTCGTCACT GGGGCCTGAT CAGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA 
CTCGATGAGG CCTGCACAGA CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCC 
CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTACT CACTCAACTG 
ATAGATGAAG CCAGTGAAGG GGATATGAGC ATTTCAATTC TTACACAGGA AATAATGGTA 
TATCTAGCCA TGTATATGCG AACTCAACCT GGCCTCTTTG CTGAAATGTT TCGACTTCGA 
ATTGGTCTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCCCTTCG ATGCTCAGCT 
GAGGAAGCCA CAGATGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAG CCTCTTGCAT 
CATATACTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCTAC TGATTCAAAT 
ATCAGCCCTG CTATTTCAAT TCGTGAGGTT GGTGCTGTTG GAGCAACCAA AACAGAACGA 
ACTGGGATCA TGCAGTTAAA AAGTGAGATA AAGCAGGTGG ACTTTCGTAG ACTGTCTATC 
TCAACTGAAA GTCAGGTGAA ACAACCACCT GGAACCTCTA TGAGTTCAAG TGGTGCGTCC 
TTCTCTAGAG CATTTGGTCA ACAGACATCT AAAGATAGTC GTCAAGGTCA ATGGCAACGC 
CGAAGAAGGC TGGATGGCGC ATTGAACAGA GTCCCAATTG GATTTTATCA AAAAGTATGG 
AAAGTTTTAC AGAAGTGTCA CGGACTTTCT GTGGAAGGTT TTGTTCTTCC TTCTTCAACT 
ACTAGAGAGA TGACTCCAGG CGAGATTAAA TTCTCTGTTC ATGTGGAGTC TGTCCTGAAT 
CGTGTACCTC AGCCAGAGTA CCGTCAGCTT CTGGTTGAAG CCATCCTTGT CCTCACCATG 
ATGGCAGACA TTGAAATTCA TAGTATTGGA AGCATCATTG CTGTGGAGAA AATAGTGCAT 
ATTGCCAATG ACTTGTTTCT TCAAGAACAG AAAACCCTCG GTGCAGATGA TATCATGTTG 
GCAAAGGATC CTGCGTCTGG CATCTGTACT CTTCTGTATG ACAGTGCACC CAGTGGCAGG 
TTTGGCACCA TGACCTACCT CTCCAAAGCA GCGGCCACCT ATGTGCAGGA GTTCCTGCCC 
CACAGCATCT GTGCCATGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_005227993.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228050.1 Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X4, mRNA.

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CloneID	OBa35461
Clone ID Related Accession (Same CDS sequence)	XM_015470418.1 , XM_005227993.3 , XM_005227993.4
Accession Version	XM_005227993.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104727.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227993.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG ATGTCCATAT GTATCTTCCT 
ACAAAGATAT TTCAGGCTTC CCGGCCTTCA TTCAACTTAC TTGGTTCACC TCATCCCCAA 
CAAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CAAGTCTGGG 
GAAGTGGACT TCAAAGCACT GGTTTTACAG TTAAAAGAGA CCTCCAGCTT ACAAGAACAA 
GCTGATATCC TCTACATGCT ATATACTATG AAAGGACCTG ACTGGGACAC TGGATTGTAT 
GATGAAGAGA GCATCACAGT CAGAGAGCTG CTTACTGAGC TATATGGCAA AGTTGGAGAA 
ATTCGTCACT GGGGCCTGAT CAGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA 
CTCGATGAGG CCTGCACAGA CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCC 
CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTACT CACTCAACTG 
ATAGATGAAG CCAGTGAAGG GGATATGAGC ATTTCAATTC TTACACAGGA AATAATGGTA 
TATCTAGCCA TGTATATGCG AACTCAACCT GGCCTCTTTG CTGAAATGTT TCGACTTCGA 
ATTGGTCTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCCCTTCG ATGCTCAGCT 
GAGGAAGCCA CAGATGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAG CCTCTTGCAT 
CATATACTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCTAC TGATTCAAAT 
ATCAGCCCTG CTATTTCAAT TCGTGAGGTT GGTGCTGTTG GAGCAACCAA AACAGAACGA 
ACTGGGATCA TGCAGTTAAA AAGTGAGATA AAGCAGGTGG ACTTTCGTAG ACTGTCTATC 
TCAACTGAAA GTCAGGTGAA ACAACCACCT GGAACCTCTA TGAGTTCAAG TGGTGCGTCC 
TTCTCTAGAG CATTTGGTCA ACAGACATCT AAAGATAGTC GTCAAGGTCA ATGGCAACGC 
CGAAGAAGGC TGGATGGCGC ATTGAACAGA GTCCCAATTG GATTTTATCA AAAAGTATGG 
AAAGTTTTAC AGAAGTGTCA CGGACTTTCT GTGGAAGGTT TTGTTCTTCC TTCTTCAACT 
ACTAGAGAGA TGACTCCAGG CGAGATTAAA TTCTCTGTTC ATGTGGAGTC TGTCCTGAAT 
CGTGTACCTC AGCCAGAGTA CCGTCAGCTT CTGGTTGAAG CCATCCTTGT CCTCACCATG 
ATGGCAGACA TTGAAATTCA TAGTATTGGA AGCATCATTG CTGTGGAGAA AATAGTGCAT 
ATTGCCAATG ACTTGTTTCT TCAAGAACAG AAAACCCTCG GTGCAGATGA TATCATGTTG 
GCAAAGGATC CTGCGTCTGG CATCTGTACT CTTCTGTATG ACAGTGCACC CAGTGGCAGG 
TTTGGCACCA TGACCTACCT CTCCAAAGCA GCGGCCACCT ATGTGCAGGA GTTCCTGCCC 
CACAGCATCT GTGCCATGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228050.1
CDS	328..3831
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSILPRASTSKEVDASLLSVISFPAFAVEDNQLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYREALEAVLIKGKNGVPLLPELYSVPPDKVDEEYKNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKSILKNKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNNMIVEMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDYDNELESGDWINGYGLSRNDVHMYLPTKIFQASRPSFNLLGSPHPQQEDQVPSVRVEIHLPRDKSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWDTGLYDEESITVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEVLTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATDGLMNLSPSAMKSLLHHILSGKEFGVERSVRPTDSNISPAISIREVGAVGATKTERTGIMQLKSEIKQVDFRRLSISTESQVKQPPGTSMSSSGASFSRAFGQQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMMADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ

Target ORF information:

RefSeq Version	XM_005227993.3
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227993.3

ORF Insert Sequence:

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG ATGTCCATAT GTATCTTCCT 
ACAAAGATAT TTCAGGCTTC CCGGCCTTCA TTCAACTTAC TTGGTTCACC TCATCCCCAA 
CAAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CAAGTCTGGG 
GAAGTGGACT TCAAAGCACT GGTTTTACAG TTAAAAGAGA CCTCCAGCTT ACAAGAACAA 
GCTGATATCC TCTACATGCT ATATACTATG AAAGGACCTG ACTGGGACAC TGGATTGTAT 
GATGAAGAGA GCATCACAGT CAGAGAGCTG CTTACTGAGC TATATGGCAA AGTTGGAGAA 
ATTCGTCACT GGGGCCTGAT CAGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA 
CTCGATGAGG CCTGCACAGA CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCC 
CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTACT CACTCAACTG 
ATAGATGAAG CCAGTGAAGG GGATATGAGC ATTTCAATTC TTACACAGGA AATAATGGTA 
TATCTAGCCA TGTATATGCG AACTCAACCT GGCCTCTTTG CTGAAATGTT TCGACTTCGA 
ATTGGTCTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCCCTTCG ATGCTCAGCT 
GAGGAAGCCA CAGATGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAG CCTCTTGCAT 
CATATACTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCTAC TGATTCAAAT 
ATCAGCCCTG CTATTTCAAT TCGTGAGGTT GGTGCTGTTG GAGCAACCAA AACAGAACGA 
ACTGGGATCA TGCAGTTAAA AAGTGAGATA AAGCAGGTGG ACTTTCGTAG ACTGTCTATC 
TCAACTGAAA GTCAGGTGAA ACAACCACCT GGAACCTCTA TGAGTTCAAG TGGTGCGTCC 
TTCTCTAGAG CATTTGGTCA ACAGACATCT AAAGATAGTC GTCAAGGTCA ATGGCAACGC 
CGAAGAAGGC TGGATGGCGC ATTGAACAGA GTCCCAATTG GATTTTATCA AAAAGTATGG 
AAAGTTTTAC AGAAGTGTCA CGGACTTTCT GTGGAAGGTT TTGTTCTTCC TTCTTCAACT 
ACTAGAGAGA TGACTCCAGG CGAGATTAAA TTCTCTGTTC ATGTGGAGTC TGTCCTGAAT 
CGTGTACCTC AGCCAGAGTA CCGTCAGCTT CTGGTTGAAG CCATCCTTGT CCTCACCATG 
ATGGCAGACA TTGAAATTCA TAGTATTGGA AGCATCATTG CTGTGGAGAA AATAGTGCAT 
ATTGCCAATG ACTTGTTTCT TCAAGAACAG AAAACCCTCG GTGCAGATGA TATCATGTTG 
GCAAAGGATC CTGCGTCTGG CATCTGTACT CTTCTGTATG ACAGTGCACC CAGTGGCAGG 
TTTGGCACCA TGACCTACCT CTCCAAAGCA GCGGCCACCT ATGTGCAGGA GTTCCTGCCC 
CACAGCATCT GTGCCATGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_005227993.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228050.1 Bos taurus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa35461
Clone ID Related Accession (Same CDS sequence)	XM_015470418.1 , XM_005227993.3 , XM_005227993.4
Accession Version	XM_005227993.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005227993.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG ATGTCCATAT GTATCTTCCT 
ACAAAGATAT TTCAGGCTTC CCGGCCTTCA TTCAACTTAC TTGGTTCACC TCATCCCCAA 
CAAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CAAGTCTGGG 
GAAGTGGACT TCAAAGCACT GGTTTTACAG TTAAAAGAGA CCTCCAGCTT ACAAGAACAA 
GCTGATATCC TCTACATGCT ATATACTATG AAAGGACCTG ACTGGGACAC TGGATTGTAT 
GATGAAGAGA GCATCACAGT CAGAGAGCTG CTTACTGAGC TATATGGCAA AGTTGGAGAA 
ATTCGTCACT GGGGCCTGAT CAGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA 
CTCGATGAGG CCTGCACAGA CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCC 
CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTACT CACTCAACTG 
ATAGATGAAG CCAGTGAAGG GGATATGAGC ATTTCAATTC TTACACAGGA AATAATGGTA 
TATCTAGCCA TGTATATGCG AACTCAACCT GGCCTCTTTG CTGAAATGTT TCGACTTCGA 
ATTGGTCTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCCCTTCG ATGCTCAGCT 
GAGGAAGCCA CAGATGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAG CCTCTTGCAT 
CATATACTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCTAC TGATTCAAAT 
ATCAGCCCTG CTATTTCAAT TCGTGAGGTT GGTGCTGTTG GAGCAACCAA AACAGAACGA 
ACTGGGATCA TGCAGTTAAA AAGTGAGATA AAGCAGGTGG ACTTTCGTAG ACTGTCTATC 
TCAACTGAAA GTCAGGTGAA ACAACCACCT GGAACCTCTA TGAGTTCAAG TGGTGCGTCC 
TTCTCTAGAG CATTTGGTCA ACAGACATCT AAAGATAGTC GTCAAGGTCA ATGGCAACGC 
CGAAGAAGGC TGGATGGCGC ATTGAACAGA GTCCCAATTG GATTTTATCA AAAAGTATGG 
AAAGTTTTAC AGAAGTGTCA CGGACTTTCT GTGGAAGGTT TTGTTCTTCC TTCTTCAACT 
ACTAGAGAGA TGACTCCAGG CGAGATTAAA TTCTCTGTTC ATGTGGAGTC TGTCCTGAAT 
CGTGTACCTC AGCCAGAGTA CCGTCAGCTT CTGGTTGAAG CCATCCTTGT CCTCACCATG 
ATGGCAGACA TTGAAATTCA TAGTATTGGA AGCATCATTG CTGTGGAGAA AATAGTGCAT 
ATTGCCAATG ACTTGTTTCT TCAAGAACAG AAAACCCTCG GTGCAGATGA TATCATGTTG 
GCAAAGGATC CTGCGTCTGG CATCTGTACT CTTCTGTATG ACAGTGCACC CAGTGGCAGG 
TTTGGCACCA TGACCTACCT CTCCAAAGCA GCGGCCACCT ATGTGCAGGA GTTCCTGCCC 
CACAGCATCT GTGCCATGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228050.1
CDS	280..3783
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSILPRASTSKEVDASLLSVISFPAFAVEDNQLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYREALEAVLIKGKNGVPLLPELYSVPPDKVDEEYKNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKSILKNKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNNMIVEMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDYDNELESGDWINGYGLSRNDVHMYLPTKIFQASRPSFNLLGSPHPQQEDQVPSVRVEIHLPRDKSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWDTGLYDEESITVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEVLTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATDGLMNLSPSAMKSLLHHILSGKEFGVERSVRPTDSNISPAISIREVGAVGATKTERTGIMQLKSEIKQVDFRRLSISTESQVKQPPGTSMSSSGASFSRAFGQQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMMADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ

Target ORF information:

RefSeq Version	XM_005227993.4
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227993.4

ORF Insert Sequence:

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG ATGTCCATAT GTATCTTCCT 
ACAAAGATAT TTCAGGCTTC CCGGCCTTCA TTCAACTTAC TTGGTTCACC TCATCCCCAA 
CAAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CAAGTCTGGG 
GAAGTGGACT TCAAAGCACT GGTTTTACAG TTAAAAGAGA CCTCCAGCTT ACAAGAACAA 
GCTGATATCC TCTACATGCT ATATACTATG AAAGGACCTG ACTGGGACAC TGGATTGTAT 
GATGAAGAGA GCATCACAGT CAGAGAGCTG CTTACTGAGC TATATGGCAA AGTTGGAGAA 
ATTCGTCACT GGGGCCTGAT CAGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA 
CTCGATGAGG CCTGCACAGA CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCC 
CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTACT CACTCAACTG 
ATAGATGAAG CCAGTGAAGG GGATATGAGC ATTTCAATTC TTACACAGGA AATAATGGTA 
TATCTAGCCA TGTATATGCG AACTCAACCT GGCCTCTTTG CTGAAATGTT TCGACTTCGA 
ATTGGTCTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCCCTTCG ATGCTCAGCT 
GAGGAAGCCA CAGATGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAG CCTCTTGCAT 
CATATACTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCTAC TGATTCAAAT 
ATCAGCCCTG CTATTTCAAT TCGTGAGGTT GGTGCTGTTG GAGCAACCAA AACAGAACGA 
ACTGGGATCA TGCAGTTAAA AAGTGAGATA AAGCAGGTGG ACTTTCGTAG ACTGTCTATC 
TCAACTGAAA GTCAGGTGAA ACAACCACCT GGAACCTCTA TGAGTTCAAG TGGTGCGTCC 
TTCTCTAGAG CATTTGGTCA ACAGACATCT AAAGATAGTC GTCAAGGTCA ATGGCAACGC 
CGAAGAAGGC TGGATGGCGC ATTGAACAGA GTCCCAATTG GATTTTATCA AAAAGTATGG 
AAAGTTTTAC AGAAGTGTCA CGGACTTTCT GTGGAAGGTT TTGTTCTTCC TTCTTCAACT 
ACTAGAGAGA TGACTCCAGG CGAGATTAAA TTCTCTGTTC ATGTGGAGTC TGTCCTGAAT 
CGTGTACCTC AGCCAGAGTA CCGTCAGCTT CTGGTTGAAG CCATCCTTGT CCTCACCATG 
ATGGCAGACA TTGAAATTCA TAGTATTGGA AGCATCATTG CTGTGGAGAA AATAGTGCAT 
ATTGCCAATG ACTTGTTTCT TCAAGAACAG AAAACCCTCG GTGCAGATGA TATCATGTTG 
GCAAAGGATC CTGCGTCTGG CATCTGTACT CTTCTGTATG ACAGTGCACC CAGTGGCAGG 
TTTGGCACCA TGACCTACCT CTCCAAAGCA GCGGCCACCT ATGTGCAGGA GTTCCTGCCC 
CACAGCATCT GTGCCATGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_003588156.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003588204.1 Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X5, mRNA.

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CloneID	OBa35462
Clone ID Related Accession (Same CDS sequence)	XM_003588156.4 , XM_003588156.5 , XM_015470419.1
Accession Version	XM_003588156.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104727.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003588156.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG ATGTCCATAT GTATCTTCCT 
ACAAAGATAT TTCAGGCTTC CCGGCCTTCA TTCAACTTAC TTGGTTCACC TCATCCCCAA 
CAAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CAAGTCTGGG 
GAAGTGGACT TCAAAGCACT GGTTTTACAG TTAAAAGAGA CCTCCAGCTT ACAAGAACAA 
GCTGATATCC TCTACATGCT ATATACTATG AAAGGACCTG ACTGGGACAC TGGATTGTAT 
GATGAAGAGA GCATCACAGT CAGAGAGCTG CTTACTGAGC TATATGGCAA AGTTGGAGAA 
ATTCGTCACT GGGGCCTGAT CAGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA 
CTCGATGAGG CCTGCACAGA CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCC 
CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTACT CACTCAACTG 
ATAGATGAAG CCAGTGAAGG GGATATGAGC ATTTCAATTC TTACACAGGA AATAATGGTA 
TATCTAGCCA TGTATATGCG AACTCAACCT GGCCTCTTTG CTGAAATGTT TCGACTTCGA 
ATTGGTCTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCCCTTCG ATGCTCAGCT 
GAGGAAGCCA CAGATGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAG CCTCTTGCAT 
CATATACTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCTAC TGATTCAAAT 
ATCAGCCCTG CTATTTCAAT TCGTGAGGTT GGTGCTGTTG GAGCAACCAA AACAGAACGA 
ACTGGGATCA TGCAGTTAAA AAGTGAGATA AAGCAGCCAC CTGGAACCTC TATGAGTTCA 
AGTGGTGCGT CCTTCTCTAG AGCATTTGGT CAACAGACAT CTAAAGATAG TCGTCAAGGT 
CAATGGCAAC GCCGAAGAAG GCTGGATGGC GCATTGAACA GAGTCCCAAT TGGATTTTAT 
CAAAAAGTAT GGAAAGTTTT ACAGAAGTGT CACGGACTTT CTGTGGAAGG TTTTGTTCTT 
CCTTCTTCAA CTACTAGAGA GATGACTCCA GGCGAGATTA AATTCTCTGT TCATGTGGAG 
TCTGTCCTGA ATCGTGTACC TCAGCCAGAG TACCGTCAGC TTCTGGTTGA AGCCATCCTT 
GTCCTCACCA TGATGGCAGA CATTGAAATT CATAGTATTG GAAGCATCAT TGCTGTGGAG 
AAAATAGTGC ATATTGCCAA TGACTTGTTT CTTCAAGAAC AGAAAACCCT CGGTGCAGAT 
GATATCATGT TGGCAAAGGA TCCTGCGTCT GGCATCTGTA CTCTTCTGTA TGACAGTGCA 
CCCAGTGGCA GGTTTGGCAC CATGACCTAC CTCTCCAAAG CAGCGGCCAC CTATGTGCAG 
GAGTTCCTGC CCCACAGCAT CTGTGCCATG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003588204.1
CDS	328..3783
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSILPRASTSKEVDASLLSVISFPAFAVEDNQLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYREALEAVLIKGKNGVPLLPELYSVPPDKVDEEYKNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKSILKNKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNNMIVEMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDYDNELESGDWINGYGLSRNDVHMYLPTKIFQASRPSFNLLGSPHPQQEDQVPSVRVEIHLPRDKSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWDTGLYDEESITVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEVLTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATDGLMNLSPSAMKSLLHHILSGKEFGVERSVRPTDSNISPAISIREVGAVGATKTERTGIMQLKSEIKQPPGTSMSSSGASFSRAFGQQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMMADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ

Target ORF information:

RefSeq Version	XM_003588156.4
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003588156.4

ORF Insert Sequence:

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG ATGTCCATAT GTATCTTCCT 
ACAAAGATAT TTCAGGCTTC CCGGCCTTCA TTCAACTTAC TTGGTTCACC TCATCCCCAA 
CAAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CAAGTCTGGG 
GAAGTGGACT TCAAAGCACT GGTTTTACAG TTAAAAGAGA CCTCCAGCTT ACAAGAACAA 
GCTGATATCC TCTACATGCT ATATACTATG AAAGGACCTG ACTGGGACAC TGGATTGTAT 
GATGAAGAGA GCATCACAGT CAGAGAGCTG CTTACTGAGC TATATGGCAA AGTTGGAGAA 
ATTCGTCACT GGGGCCTGAT CAGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA 
CTCGATGAGG CCTGCACAGA CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCC 
CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTACT CACTCAACTG 
ATAGATGAAG CCAGTGAAGG GGATATGAGC ATTTCAATTC TTACACAGGA AATAATGGTA 
TATCTAGCCA TGTATATGCG AACTCAACCT GGCCTCTTTG CTGAAATGTT TCGACTTCGA 
ATTGGTCTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCCCTTCG ATGCTCAGCT 
GAGGAAGCCA CAGATGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAG CCTCTTGCAT 
CATATACTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCTAC TGATTCAAAT 
ATCAGCCCTG CTATTTCAAT TCGTGAGGTT GGTGCTGTTG GAGCAACCAA AACAGAACGA 
ACTGGGATCA TGCAGTTAAA AAGTGAGATA AAGCAGCCAC CTGGAACCTC TATGAGTTCA 
AGTGGTGCGT CCTTCTCTAG AGCATTTGGT CAACAGACAT CTAAAGATAG TCGTCAAGGT 
CAATGGCAAC GCCGAAGAAG GCTGGATGGC GCATTGAACA GAGTCCCAAT TGGATTTTAT 
CAAAAAGTAT GGAAAGTTTT ACAGAAGTGT CACGGACTTT CTGTGGAAGG TTTTGTTCTT 
CCTTCTTCAA CTACTAGAGA GATGACTCCA GGCGAGATTA AATTCTCTGT TCATGTGGAG 
TCTGTCCTGA ATCGTGTACC TCAGCCAGAG TACCGTCAGC TTCTGGTTGA AGCCATCCTT 
GTCCTCACCA TGATGGCAGA CATTGAAATT CATAGTATTG GAAGCATCAT TGCTGTGGAG 
AAAATAGTGC ATATTGCCAA TGACTTGTTT CTTCAAGAAC AGAAAACCCT CGGTGCAGAT 
GATATCATGT TGGCAAAGGA TCCTGCGTCT GGCATCTGTA CTCTTCTGTA TGACAGTGCA 
CCCAGTGGCA GGTTTGGCAC CATGACCTAC CTCTCCAAAG CAGCGGCCAC CTATGTGCAG 
GAGTTCCTGC CCCACAGCAT CTGTGCCATG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_003588156.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003588204.1 Bos taurus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X6, mRNA.

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CloneID	OBa35462
Clone ID Related Accession (Same CDS sequence)	XM_003588156.4 , XM_003588156.5 , XM_015470419.1
Accession Version	XM_003588156.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003588156.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG ATGTCCATAT GTATCTTCCT 
ACAAAGATAT TTCAGGCTTC CCGGCCTTCA TTCAACTTAC TTGGTTCACC TCATCCCCAA 
CAAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CAAGTCTGGG 
GAAGTGGACT TCAAAGCACT GGTTTTACAG TTAAAAGAGA CCTCCAGCTT ACAAGAACAA 
GCTGATATCC TCTACATGCT ATATACTATG AAAGGACCTG ACTGGGACAC TGGATTGTAT 
GATGAAGAGA GCATCACAGT CAGAGAGCTG CTTACTGAGC TATATGGCAA AGTTGGAGAA 
ATTCGTCACT GGGGCCTGAT CAGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA 
CTCGATGAGG CCTGCACAGA CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCC 
CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTACT CACTCAACTG 
ATAGATGAAG CCAGTGAAGG GGATATGAGC ATTTCAATTC TTACACAGGA AATAATGGTA 
TATCTAGCCA TGTATATGCG AACTCAACCT GGCCTCTTTG CTGAAATGTT TCGACTTCGA 
ATTGGTCTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCCCTTCG ATGCTCAGCT 
GAGGAAGCCA CAGATGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAG CCTCTTGCAT 
CATATACTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCTAC TGATTCAAAT 
ATCAGCCCTG CTATTTCAAT TCGTGAGGTT GGTGCTGTTG GAGCAACCAA AACAGAACGA 
ACTGGGATCA TGCAGTTAAA AAGTGAGATA AAGCAGCCAC CTGGAACCTC TATGAGTTCA 
AGTGGTGCGT CCTTCTCTAG AGCATTTGGT CAACAGACAT CTAAAGATAG TCGTCAAGGT 
CAATGGCAAC GCCGAAGAAG GCTGGATGGC GCATTGAACA GAGTCCCAAT TGGATTTTAT 
CAAAAAGTAT GGAAAGTTTT ACAGAAGTGT CACGGACTTT CTGTGGAAGG TTTTGTTCTT 
CCTTCTTCAA CTACTAGAGA GATGACTCCA GGCGAGATTA AATTCTCTGT TCATGTGGAG 
TCTGTCCTGA ATCGTGTACC TCAGCCAGAG TACCGTCAGC TTCTGGTTGA AGCCATCCTT 
GTCCTCACCA TGATGGCAGA CATTGAAATT CATAGTATTG GAAGCATCAT TGCTGTGGAG 
AAAATAGTGC ATATTGCCAA TGACTTGTTT CTTCAAGAAC AGAAAACCCT CGGTGCAGAT 
GATATCATGT TGGCAAAGGA TCCTGCGTCT GGCATCTGTA CTCTTCTGTA TGACAGTGCA 
CCCAGTGGCA GGTTTGGCAC CATGACCTAC CTCTCCAAAG CAGCGGCCAC CTATGTGCAG 
GAGTTCCTGC CCCACAGCAT CTGTGCCATG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003588204.1
CDS	112..3567
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSILPRASTSKEVDASLLSVISFPAFAVEDNQLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYREALEAVLIKGKNGVPLLPELYSVPPDKVDEEYKNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKSILKNKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNNMIVEMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDYDNELESGDWINGYGLSRNDVHMYLPTKIFQASRPSFNLLGSPHPQQEDQVPSVRVEIHLPRDKSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWDTGLYDEESITVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEVLTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATDGLMNLSPSAMKSLLHHILSGKEFGVERSVRPTDSNISPAISIREVGAVGATKTERTGIMQLKSEIKQPPGTSMSSSGASFSRAFGQQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMMADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ

Target ORF information:

RefSeq Version	XM_003588156.5
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003588156.5

ORF Insert Sequence:

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG ATGTCCATAT GTATCTTCCT 
ACAAAGATAT TTCAGGCTTC CCGGCCTTCA TTCAACTTAC TTGGTTCACC TCATCCCCAA 
CAAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CAAGTCTGGG 
GAAGTGGACT TCAAAGCACT GGTTTTACAG TTAAAAGAGA CCTCCAGCTT ACAAGAACAA 
GCTGATATCC TCTACATGCT ATATACTATG AAAGGACCTG ACTGGGACAC TGGATTGTAT 
GATGAAGAGA GCATCACAGT CAGAGAGCTG CTTACTGAGC TATATGGCAA AGTTGGAGAA 
ATTCGTCACT GGGGCCTGAT CAGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA 
CTCGATGAGG CCTGCACAGA CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCC 
CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTACT CACTCAACTG 
ATAGATGAAG CCAGTGAAGG GGATATGAGC ATTTCAATTC TTACACAGGA AATAATGGTA 
TATCTAGCCA TGTATATGCG AACTCAACCT GGCCTCTTTG CTGAAATGTT TCGACTTCGA 
ATTGGTCTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCCCTTCG ATGCTCAGCT 
GAGGAAGCCA CAGATGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAG CCTCTTGCAT 
CATATACTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCTAC TGATTCAAAT 
ATCAGCCCTG CTATTTCAAT TCGTGAGGTT GGTGCTGTTG GAGCAACCAA AACAGAACGA 
ACTGGGATCA TGCAGTTAAA AAGTGAGATA AAGCAGCCAC CTGGAACCTC TATGAGTTCA 
AGTGGTGCGT CCTTCTCTAG AGCATTTGGT CAACAGACAT CTAAAGATAG TCGTCAAGGT 
CAATGGCAAC GCCGAAGAAG GCTGGATGGC GCATTGAACA GAGTCCCAAT TGGATTTTAT 
CAAAAAGTAT GGAAAGTTTT ACAGAAGTGT CACGGACTTT CTGTGGAAGG TTTTGTTCTT 
CCTTCTTCAA CTACTAGAGA GATGACTCCA GGCGAGATTA AATTCTCTGT TCATGTGGAG 
TCTGTCCTGA ATCGTGTACC TCAGCCAGAG TACCGTCAGC TTCTGGTTGA AGCCATCCTT 
GTCCTCACCA TGATGGCAGA CATTGAAATT CATAGTATTG GAAGCATCAT TGCTGTGGAG 
AAAATAGTGC ATATTGCCAA TGACTTGTTT CTTCAAGAAC AGAAAACCCT CGGTGCAGAT 
GATATCATGT TGGCAAAGGA TCCTGCGTCT GGCATCTGTA CTCTTCTGTA TGACAGTGCA 
CCCAGTGGCA GGTTTGGCAC CATGACCTAC CTCTCCAAAG CAGCGGCCAC CTATGTGCAG 
GAGTTCCTGC CCCACAGCAT CTGTGCCATG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_015470419.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325905.1 Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa35462
Clone ID Related Accession (Same CDS sequence)	XM_003588156.4 , XM_003588156.5 , XM_015470419.1
Accession Version	XM_015470419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647134.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG ATGTCCATAT GTATCTTCCT 
ACAAAGATAT TTCAGGCTTC CCGGCCTTCA TTCAACTTAC TTGGTTCACC TCATCCCCAA 
CAAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CAAGTCTGGG 
GAAGTGGACT TCAAAGCACT GGTTTTACAG TTAAAAGAGA CCTCCAGCTT ACAAGAACAA 
GCTGATATCC TCTACATGCT ATATACTATG AAAGGACCTG ACTGGGACAC TGGATTGTAT 
GATGAAGAGA GCATCACAGT CAGAGAGCTG CTTACTGAGC TATATGGCAA AGTTGGAGAA 
ATTCGTCACT GGGGCCTGAT CAGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA 
CTCGATGAGG CCTGCACAGA CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCC 
CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTACT CACTCAACTG 
ATAGATGAAG CCAGTGAAGG GGATATGAGC ATTTCAATTC TTACACAGGA AATAATGGTA 
TATCTAGCCA TGTATATGCG AACTCAACCT GGCCTCTTTG CTGAAATGTT TCGACTTCGA 
ATTGGTCTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCCCTTCG ATGCTCAGCT 
GAGGAAGCCA CAGATGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAG CCTCTTGCAT 
CATATACTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCTAC TGATTCAAAT 
ATCAGCCCTG CTATTTCAAT TCGTGAGGTT GGTGCTGTTG GAGCAACCAA AACAGAACGA 
ACTGGGATCA TGCAGTTAAA AAGTGAGATA AAGCAGCCAC CTGGAACCTC TATGAGTTCA 
AGTGGTGCGT CCTTCTCTAG AGCATTTGGT CAACAGACAT CTAAAGATAG TCGTCAAGGT 
CAATGGCAAC GCCGAAGAAG GCTGGATGGC GCATTGAACA GAGTCCCAAT TGGATTTTAT 
CAAAAAGTAT GGAAAGTTTT ACAGAAGTGT CACGGACTTT CTGTGGAAGG TTTTGTTCTT 
CCTTCTTCAA CTACTAGAGA GATGACTCCA GGCGAGATTA AATTCTCTGT TCATGTGGAG 
TCTGTCCTGA ATCGTGTACC TCAGCCAGAG TACCGTCAGC TTCTGGTTGA AGCCATCCTT 
GTCCTCACCA TGATGGCAGA CATTGAAATT CATAGTATTG GAAGCATCAT TGCTGTGGAG 
AAAATAGTGC ATATTGCCAA TGACTTGTTT CTTCAAGAAC AGAAAACCCT CGGTGCAGAT 
GATATCATGT TGGCAAAGGA TCCTGCGTCT GGCATCTGTA CTCTTCTGTA TGACAGTGCA 
CCCAGTGGCA GGTTTGGCAC CATGACCTAC CTCTCCAAAG CAGCGGCCAC CTATGTGCAG 
GAGTTCCTGC CCCACAGCAT CTGTGCCATG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325905.1
CDS	328..3783
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSILPRASTSKEVDASLLSVISFPAFAVEDNQLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYREALEAVLIKGKNGVPLLPELYSVPPDKVDEEYKNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKSILKNKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNNMIVEMLRTDLSYLCSRWRMTGQPTITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDYDNELESGDWINGYGLSRNDVHMYLPTKIFQASRPSFNLLGSPHPQQEDQVPSVRVEIHLPRDKSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWDTGLYDEESITVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEVLTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATDGLMNLSPSAMKSLLHHILSGKEFGVERSVRPTDSNISPAISIREVGAVGATKTERTGIMQLKSEIKQPPGTSMSSSGASFSRAFGQQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMMADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSICAMQ

Target ORF information:

RefSeq Version	XM_015470419.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470419.1

ORF Insert Sequence:

ATGAGGAGTC GGAGTAACTC CGGGGTCCGG CTAGACGGCT ATGCTAGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA CCCAGTGACT GGCTTACTTC CAGCCAGCTA TGACCAGAAA 
GATGCCTGGG TCCGGGATAA TGTGTACAGC ATCTTGGCTG TGTGGGGTTT GGGCCTGGCC 
TATCGGAAGA ATGCAGACCG AGATGAGGAT AAGGCAAAAG CCTATGAACT GGAACAGAGT 
GTAGTGAAGC TGATGAGGGG ACTGCTGCAC TGCATGATTA GACAGGTGGA TAAAGTAGAA 
TCCTTCAAGT ATAGTCAGAG TACCAAGGAT AGCCTCCATG CCAAGTACAA CACCAAAACC 
TGTGCCACTG TAGTGGGTGA TGATCAGTGG GGACACCTGC AGTTGGATGC TACCTCTGTG 
TACCTGCTCT TCTTAGCACA AATGACTGCC TCAGGACTTC ATATTATCCA CAGCCTAGAT 
GAAGTTAATT TCATACAGAA CCTTGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTCGGGATAT GGGAACGTGG AGACAAGACC AATCAAGGAA TCTCAGAATT GAATGCCAGT 
TCAGTTGGAA TGGCAAAGGC AGCCCTGGAA GCATTAGATG AACTGGACCT GTTTGGTGTG 
AAGGGTGGGC CGCAGTCAGT TATCCATGTC CTAGCTGATG AAGTACAACA CTGCCAGTCT 
ATCCTTAATT CAATACTCCC CCGGGCTTCA ACATCTAAAG AAGTTGATGC TAGTTTACTT 
TCAGTTATTT CCTTCCCAGC CTTTGCAGTA GAGGATAACC AGTTGGTGGA GCTCACAAAA 
CAGGAAATCA TCACCAAGCT TCAAGGTCGT TATGGTTGTT GTCGCTTTCT ACGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGT CTGTACTATG AACCAGCTGA GCTGAAGTTA 
TTTGAAAACA TTGAGTGTGA ATGGCCGTTG TTCTGGACAT ACTTTATCCT CGATGGGGTC 
TTCAGTGGCA ATGCAGAACA GGTTCAAGAA TACAGAGAAG CTCTTGAAGC AGTCCTCATC 
AAGGGCAAAA ACGGTGTCCC ACTTCTGCCA GAGTTGTACA GTGTTCCTCC TGACAAGGTT 
GATGAAGAAT ATAAAAATCC TCACACTGTG GACCGAGTCC CCATGGGCAA ACTGCCTCAT 
ATGTGGGGAC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTAAA TCGTAGGTTT TCTACTGTAC CAAAGCCAGA TGTTGTGGTT 
CAAGTTTCCA TTCTAGCTGA AACAGAAGAA ATCAAGTCCA TTTTGAAGAA CAAGGGAATT 
GATGTGGAGA CCATTGCTGA GGTATATCCC ATCAGAGTAC AACCAGCTCG TATTCTCAGC 
CACATTTATT CCAGCCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGAAG ACCCTACAGA 
CACATGGGTG TCCTTGGAAC TTCAAAACTC TATGACATTC GGAAAACTAT CTTTACCTTC 
ACTCCCCAGT TTATAGACCA GCAACAATTC TACCTGGCTC TCGACAACAA CATGATAGTG 
GAAATGCTTA GAACAGACCT CTCCTATCTC TGTAGCCGCT GGAGAATGAC AGGCCAGCCC 
ACCATCACCT TCCCCATCTC ACACAGCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG AAAAATGCAG GATGGATATT TTGGTGGGGC AAGGATTCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGTTGCA CACATTTGAG CTTCATGGAC 
CCTGGACCTG AGGGTAAACT GTACAGTGAA GACTATGATG ACTATGATAA TGAGCTGGAA 
TCTGGTGACT GGATTAATGG CTATGGTTTA TCCCGTAATG ATGTCCATAT GTATCTTCCT 
ACAAAGATAT TTCAGGCTTC CCGGCCTTCA TTCAACTTAC TTGGTTCACC TCATCCCCAA 
CAAGAGGACC AGGTTCCCTC TGTTCGTGTA GAAATACATC TTCCTAGAGA CAAGTCTGGG 
GAAGTGGACT TCAAAGCACT GGTTTTACAG TTAAAAGAGA CCTCCAGCTT ACAAGAACAA 
GCTGATATCC TCTACATGCT ATATACTATG AAAGGACCTG ACTGGGACAC TGGATTGTAT 
GATGAAGAGA GCATCACAGT CAGAGAGCTG CTTACTGAGC TATATGGCAA AGTTGGAGAA 
ATTCGTCACT GGGGCCTGAT CAGATACATC TCTGGAATCT TAAGGAAGAA GGTGGAAGCA 
CTCGATGAGG CCTGCACAGA CCTTCTCTCC CATCAGAAAC ATTTGACAGT GGGACTCCCC 
CCAGAACCTC GAGAGAAGAC CATCTCTGCA CCTCTGCCCT ATGAAGTACT CACTCAACTG 
ATAGATGAAG CCAGTGAAGG GGATATGAGC ATTTCAATTC TTACACAGGA AATAATGGTA 
TATCTAGCCA TGTATATGCG AACTCAACCT GGCCTCTTTG CTGAAATGTT TCGACTTCGA 
ATTGGTCTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCCCTTCG ATGCTCAGCT 
GAGGAAGCCA CAGATGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAG CCTCTTGCAT 
CATATACTCA GTGGCAAGGA GTTTGGAGTG GAACGAAGTG TTCGTCCTAC TGATTCAAAT 
ATCAGCCCTG CTATTTCAAT TCGTGAGGTT GGTGCTGTTG GAGCAACCAA AACAGAACGA 
ACTGGGATCA TGCAGTTAAA AAGTGAGATA AAGCAGCCAC CTGGAACCTC TATGAGTTCA 
AGTGGTGCGT CCTTCTCTAG AGCATTTGGT CAACAGACAT CTAAAGATAG TCGTCAAGGT 
CAATGGCAAC GCCGAAGAAG GCTGGATGGC GCATTGAACA GAGTCCCAAT TGGATTTTAT 
CAAAAAGTAT GGAAAGTTTT ACAGAAGTGT CACGGACTTT CTGTGGAAGG TTTTGTTCTT 
CCTTCTTCAA CTACTAGAGA GATGACTCCA GGCGAGATTA AATTCTCTGT TCATGTGGAG 
TCTGTCCTGA ATCGTGTACC TCAGCCAGAG TACCGTCAGC TTCTGGTTGA AGCCATCCTT 
GTCCTCACCA TGATGGCAGA CATTGAAATT CATAGTATTG GAAGCATCAT TGCTGTGGAG 
AAAATAGTGC ATATTGCCAA TGACTTGTTT CTTCAAGAAC AGAAAACCCT CGGTGCAGAT 
GATATCATGT TGGCAAAGGA TCCTGCGTCT GGCATCTGTA CTCTTCTGTA TGACAGTGCA 
CCCAGTGGCA GGTTTGGCAC CATGACCTAC CTCTCCAAAG CAGCGGCCAC CTATGTGCAG 
GAGTTCCTGC CCCACAGCAT CTGTGCCATG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PHKA1
Entrez Gene ID	100336808
Full Name	phosphorylase kinase regulatory subunit alpha 1
General protein information	Preferred Names phosphorylase kinase regulatory subunit alpha 1 Names phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform phosphorylase kinase, alpha 1 (muscle)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Danio rerio (zebrafish)	phka1	XP_005166575.1
Pan troglodytes (chimpanzee)	PHKA1	XP_003317571.2
Mus musculus (house mouse)	Phka1	NP_032858.2
Gallus gallus (chicken)	PHKA1	XP_004940655.1
	PHKA1	XP_001094193.1
Xenopus tropicalis (tropical clawed frog)	phka1	NP_001121510.1
Homo sapiens (human)	PHKA1	NP_002628.2
Canis lupus familiaris (dog)	PHKA1	XP_005641551.1
Bos taurus (cattle)	PHKA1	XP_005228049.1
Rattus norvegicus (Norway rat)	Phka1	NP_072148.1
Danio rerio (zebrafish)	si:dkey-22f5.10	XP_690880.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PHKA1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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