RASAL1 cDNA ORF clone, Bos indicus(Bos taurus indicus)

Gene
Clones
gRNAs

Summary

Gene Symbol	RASAL1
Entrez Gene ID	109571294
Full Name	RAS protein activator like 1
Gene Type	protein-coding
Organism	Bos indicus(Bos taurus indicus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019977659.1	XP_019833218.1	rasGAP-activating-like protein 1 isoform X1
XM_019977660.1	XP_019833219.1	rasGAP-activating-like protein 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following RASAL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASAL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo34534	XM_019977659.1 Latest version!	Bos indicus RAS protein activator like 1 (RASAL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBo34535	XM_019977660.1 Latest version!	Bos indicus RAS protein activator like 1 (RASAL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASAL1 ( XM_019977659.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019833218.1 Bos indicus RAS protein activator like 1 (RASAL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBo34534
Clone ID Related Accession (Same CDS sequence)	XM_019977659.1
Accession Version	XM_019977659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	rasGAP-activating-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032666.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCC TGCCGCGGTC CAGCCTCGGA ACCCGGGCGC CCTCCTGGTC GGAAAGTTTG 
GGGCAGGGCG CCATGGCCAA GAGCAGCTCC CTGAACGTCC GCGTCGTGGA GGGCCGGGCG 
CTGCCCGCCA AGGATGTGTC RGGGAGCAGT GACCCCTACT GCATTGTGAA AGTGGATGAC 
GAAGTTGTGG CCAGGACAGC AACCATCTGG CGGAGCCTGA GCCCCTTCTG GGGGGAGGAG 
TACACCGTGM ACTTGCCCCT GGATTTCCAC CATCTGTCCT TCTACGTGCT GGACGAGGAC 
ACAGTGGGGC AAGATGACAT CATAGGCAAG ATCTCGCTGA GCAGGGAGGC CATTACAGCG 
GACCCCCGAG GAATCGACAG CTGGATCAAC CTGAGCCGCG TGGACCCGGA CTCGGAGGTG 
CAGGGTGAGA TCTGCCTGTC CGTGCAGACG CTGGAGGACG TGCGGGGCCG TTGTCTTCAC 
TGTCACGTGC TCCAGGCTAG GGACCTGGCC CCCCGAGACA TCTCCGGCAC GTCTGACCCA 
TTTGCGCGTG TGTTTTGGGG CAGCCAGAGC TTGGAGACTT CGACCATCAA GAAGACTCGC 
TTCCCACACT GGGATGAGGT GCTGGAGCTG CGGGAGATGC CAGGTTCCCC ATCCCCTCTG 
CGAGTGGAAC TCTGGGACTG GGACATGGTT GGCAAGAATG ACTTTTTGGG AATGGTGGAG 
TTCCCCCCAC AGGTCCTGCA ACACAACCCG CCCAATGGCT GGTTCCGCCT CCTGCCCTTT 
CCCAGAGCAG AGGAGGATTC TGGCGGGAGC TTGGGTGCTC TGAGGCTGAA GGTGCGCCTG 
ACCGAGGACT CTGTCCTGCC CTCACGGTAC TACCAGCCAC TTAGGGAGCT GCTCATGGAG 
TCGGTGCTGG GGCCAGCAGA GGAGGATGCC GCCAGCCCCT TGGCTGTCCT GGAGGAGTTG 
ACCTCGGGTG ACTGCCGCCA GGAGCTGGCC ACCAAGCTGG TGAAGCTCTT TCTGGGCCAG 
GGCCTGACTG GGCCCTTTCT GGACTATCTC ACCCGGCGCG AGGTGGCTCG GACCACTGAC 
CCCAACACCC TCTTCCGTTC TAACTCCCTG GCGTCCAAGT CAGTGGAGCA GTTTATGAAG 
CTCGTGGGCA TGCCCTATCT GCATGAGGTC CTAAGGCCAG TGATCAACCG CGTCTTCGAG 
GAGAGGAAGT ACATGGAGCT GGATCCCTGC AAGATGGACC TGGGCCGCAC CCGGAGAATC 
TCCTTCAAGG GTGCACCCTC CGAGGAACAC GTGCGTGAGG CCAGCCTGGG GCTGCTGACG 
GGCTACCTGG GGCCTATYGT GGACGCCATT GTGGGCTCCG TGGGGCGCTG CCCATCCGCC 
ATGCGCCTCG CCTTCAAGCA GCTGCGCCAG CGCGTGGAGG AGCGTTTTCC CCAGGCGGAG 
CATGAGGACG TGAAGTACCT GGCCATAAGC GGCTTTCTCT TCCTGCGATT CTTCGCACCT 
GCCATCCTCT CTCCAAAGCT GTTTGACCTC CGGGACCAGC ACGCGGATCC CCAGACCAGC 
CGCTCACTGC TGCTGCTTGC CAAGGCTGTG CAGAGCATTG GAAATCTGGG CCAGCAGCTG 
GGCCAGGGCA AGGAGCTGTG GATGGCCCCC CTACACCCCT TYCTGCTGCA GAGTATTTCC 
AGGGTGAGAG ACTTCCTGGA CCAGCTGGTG GAGGTGGATG GGAAGGAGGA GGCTGGGGGC 
CCAGCCAGGG CCCTGGTCCC GCCCTCAATG ACTGTCCGAG AAGGCTACCT ACTCAAGCGC 
AAGGAGGAGC CTGCCGGCCT GGCCACACGC TTTGCCTTCA AGAAGCGCTA CTTCCGGCTC 
AGCGGGGAGA TGCTGTCCTA CTCCAAGAGT CCTGAGTGGC AGRTGCGCTC GTCCATCCCC 
GTGTCGCACA TCCGAGCCGT GGAGCGCGTG GACGAGGGCG CCTTCCAGCT GCCGCACGTG 
ATGCAGGTGG TGACGCAGGA CGGCGCGGGG GCGCCGCACA CCACCTACCT CCAGTGCAAG 
AACGTGAACG AGCTCAACCA ATGGCTGTCG GCCCTGCGCA AGGCCAGCGC CCCCAACCCG 
GACAAGCTGG CCTCCTRCCA CCCCGGTGCC TTCCGCAGCG GGCACTGGAC CTGCTGCCTC 
CAGGCCGAGC GCTCAGCTTC CGGTTGCAGC CGCACGCACT CGGCCGTCAC CCTGGGGGAC 
TGGAGTGACC CGCTGGATCC CGATGCTGAG ACCCAGATGG TGTATCGGCA GCTGCTTCTG 
GGGMGGGACC GGCTCAGGAT GAAATTCCTT GAGGATTCCA ACATGGATAC AACTCTGGAG 
GCAGCCACAG AGCAGGGCTC CAGTGCCATG GAAGGGGCCT GTACTGATGC CCTGGCTCGG 
CAGAGGGAGG CAGCTGCCCG CCTGCTGAAG GTGCTCACAG ACCTGGACCA AGCCCACGAG 
GAGTTCCAGC AGCAGGAACA GGGGAAGGTG GTTTCGGGCC CCCTGAGGCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019833218.1
CDS	629..3142
Translation	MPSLPRSSLGTRAPSWSESLGQGAMAKSSSLNVRVVEGRALPAKDVnullGSSDPYCIVKVDDEVVARTATIWRSLSPFWGEEYTVnullLPLDFHHLSFYVLDEDTVGQDDIIGKISLSREAITADPRGIDSWINLSRVDPDSEVQGEICLSVQTLEDVRGRCLHCHVLQARDLAPRDISGTSDPFARVFWGSQSLETSTIKKTRFPHWDEVLELREMPGSPSPLRVELWDWDMVGKNDFLGMVEFPPQVLQHNPPNGWFRLLPFPRAEEDSGGSLGALRLKVRLTEDSVLPSRYYQPLRELLMESVLGPAEEDAASPLAVLEELTSGDCRQELATKLVKLFLGQGLTGPFLDYLTRREVARTTDPNTLFRSNSLASKSVEQFMKLVGMPYLHEVLRPVINRVFEERKYMELDPCKMDLGRTRRISFKGAPSEEHVREASLGLLTGYLGPnullVDAIVGSVGRCPSAMRLAFKQLRQRVEERFPQAEHEDVKYLAISGFLFLRFFAPAILSPKLFDLRDQHADPQTSRSLLLLAKAVQSIGNLGQQLGQGKELWMAPLHPnullLLQSISRVRDFLDQLVEVDGKEEAGGPARALVPPSMTVREGYLLKRKEEPAGLATRFAFKKRYFRLSGEMLSYSKSPEWQnullRSSIPVSHIRAVERVDEGAFQLPHVMQVVTQDGAGAPHTTYLQCKNVNELNQWLSALRKASAPNPDKLASnullHPGAFRSGHWTCCLQAERSASGCSRTHSAVTLGDWSDPLDPDAETQMVYRQLLLGnullDRLRMKFLEDSNMDTTLEAATEQGSSAMEGACTDALARQREAAARLLKVLTDLDQAHEEFQQQEQGKVVSGPLRP

Target ORF information:

RefSeq Version	XM_019977659.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus RAS protein activator like 1 (RASAL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019977659.1

ORF Insert Sequence:

ATGCCCTCCC TGCCGCGGTC CAGCCTCGGA ACCCGGGCGC CCTCCTGGTC GGAAAGTTTG 
GGGCAGGGCG CCATGGCCAA GAGCAGCTCC CTGAACGTCC GCGTCGTGGA GGGCCGGGCG 
CTGCCCGCCA AGGATGTGTC RGGGAGCAGT GACCCCTACT GCATTGTGAA AGTGGATGAC 
GAAGTTGTGG CCAGGACAGC AACCATCTGG CGGAGCCTGA GCCCCTTCTG GGGGGAGGAG 
TACACCGTGM ACTTGCCCCT GGATTTCCAC CATCTGTCCT TCTACGTGCT GGACGAGGAC 
ACAGTGGGGC AAGATGACAT CATAGGCAAG ATCTCGCTGA GCAGGGAGGC CATTACAGCG 
GACCCCCGAG GAATCGACAG CTGGATCAAC CTGAGCCGCG TGGACCCGGA CTCGGAGGTG 
CAGGGTGAGA TCTGCCTGTC CGTGCAGACG CTGGAGGACG TGCGGGGCCG TTGTCTTCAC 
TGTCACGTGC TCCAGGCTAG GGACCTGGCC CCCCGAGACA TCTCCGGCAC GTCTGACCCA 
TTTGCGCGTG TGTTTTGGGG CAGCCAGAGC TTGGAGACTT CGACCATCAA GAAGACTCGC 
TTCCCACACT GGGATGAGGT GCTGGAGCTG CGGGAGATGC CAGGTTCCCC ATCCCCTCTG 
CGAGTGGAAC TCTGGGACTG GGACATGGTT GGCAAGAATG ACTTTTTGGG AATGGTGGAG 
TTCCCCCCAC AGGTCCTGCA ACACAACCCG CCCAATGGCT GGTTCCGCCT CCTGCCCTTT 
CCCAGAGCAG AGGAGGATTC TGGCGGGAGC TTGGGTGCTC TGAGGCTGAA GGTGCGCCTG 
ACCGAGGACT CTGTCCTGCC CTCACGGTAC TACCAGCCAC TTAGGGAGCT GCTCATGGAG 
TCGGTGCTGG GGCCAGCAGA GGAGGATGCC GCCAGCCCCT TGGCTGTCCT GGAGGAGTTG 
ACCTCGGGTG ACTGCCGCCA GGAGCTGGCC ACCAAGCTGG TGAAGCTCTT TCTGGGCCAG 
GGCCTGACTG GGCCCTTTCT GGACTATCTC ACCCGGCGCG AGGTGGCTCG GACCACTGAC 
CCCAACACCC TCTTCCGTTC TAACTCCCTG GCGTCCAAGT CAGTGGAGCA GTTTATGAAG 
CTCGTGGGCA TGCCCTATCT GCATGAGGTC CTAAGGCCAG TGATCAACCG CGTCTTCGAG 
GAGAGGAAGT ACATGGAGCT GGATCCCTGC AAGATGGACC TGGGCCGCAC CCGGAGAATC 
TCCTTCAAGG GTGCACCCTC CGAGGAACAC GTGCGTGAGG CCAGCCTGGG GCTGCTGACG 
GGCTACCTGG GGCCTATYGT GGACGCCATT GTGGGCTCCG TGGGGCGCTG CCCATCCGCC 
ATGCGCCTCG CCTTCAAGCA GCTGCGCCAG CGCGTGGAGG AGCGTTTTCC CCAGGCGGAG 
CATGAGGACG TGAAGTACCT GGCCATAAGC GGCTTTCTCT TCCTGCGATT CTTCGCACCT 
GCCATCCTCT CTCCAAAGCT GTTTGACCTC CGGGACCAGC ACGCGGATCC CCAGACCAGC 
CGCTCACTGC TGCTGCTTGC CAAGGCTGTG CAGAGCATTG GAAATCTGGG CCAGCAGCTG 
GGCCAGGGCA AGGAGCTGTG GATGGCCCCC CTACACCCCT TYCTGCTGCA GAGTATTTCC 
AGGGTGAGAG ACTTCCTGGA CCAGCTGGTG GAGGTGGATG GGAAGGAGGA GGCTGGGGGC 
CCAGCCAGGG CCCTGGTCCC GCCCTCAATG ACTGTCCGAG AAGGCTACCT ACTCAAGCGC 
AAGGAGGAGC CTGCCGGCCT GGCCACACGC TTTGCCTTCA AGAAGCGCTA CTTCCGGCTC 
AGCGGGGAGA TGCTGTCCTA CTCCAAGAGT CCTGAGTGGC AGRTGCGCTC GTCCATCCCC 
GTGTCGCACA TCCGAGCCGT GGAGCGCGTG GACGAGGGCG CCTTCCAGCT GCCGCACGTG 
ATGCAGGTGG TGACGCAGGA CGGCGCGGGG GCGCCGCACA CCACCTACCT CCAGTGCAAG 
AACGTGAACG AGCTCAACCA ATGGCTGTCG GCCCTGCGCA AGGCCAGCGC CCCCAACCCG 
GACAAGCTGG CCTCCTRCCA CCCCGGTGCC TTCCGCAGCG GGCACTGGAC CTGCTGCCTC 
CAGGCCGAGC GCTCAGCTTC CGGTTGCAGC CGCACGCACT CGGCCGTCAC CCTGGGGGAC 
TGGAGTGACC CGCTGGATCC CGATGCTGAG ACCCAGATGG TGTATCGGCA GCTGCTTCTG 
GGGMGGGACC GGCTCAGGAT GAAATTCCTT GAGGATTCCA ACATGGATAC AACTCTGGAG 
GCAGCCACAG AGCAGGGCTC CAGTGCCATG GAAGGGGCCT GTACTGATGC CCTGGCTCGG 
CAGAGGGAGG CAGCTGCCCG CCTGCTGAAG GTGCTCACAG ACCTGGACCA AGCCCACGAG 
GAGTTCCAGC AGCAGGAACA GGGGAAGGTG GTTTCGGGCC CCCTGAGGCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL1 ( XM_019977660.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019833219.1 Bos indicus RAS protein activator like 1 (RASAL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBo34535
Clone ID Related Accession (Same CDS sequence)	XM_019977660.1
Accession Version	XM_019977660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	rasGAP-activating-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032666.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAC TGGCCTGTTG GACAGCAACC ATCTGGCGGA GCCTGAGCCC CTTCTGGGGG 
GAGGAGTACA CCGTGMACTT GCCCCTGGAT TTCCACCATC TGTCCTTCTA CGTGCTGGAC 
GAGGACACAG TGGGGCAAGA TGACATCATA GGCAAGATCT CGCTGAGCAG GGAGGCCATT 
ACAGCGGACC CCCGAGGAAT CGACAGCTGG ATCAACCTGA GCCGCGTGGA CCCGGACTCG 
GAGGTGCAGG GTGAGATCTG CCTGTCCGTG CAGACGCTGG AGGACGTGCG GGGCCGTTGT 
CTTCACTGTC ACGTGCTCCA GGCTAGGGAC CTGGCCCCCC GAGACATCTC CGGCACGTCT 
GACCCATTTG CGCGTGTGTT TTGGGGCAGC CAGAGCTTGG AGACTTCGAC CATCAAGAAG 
ACTCGCTTCC CACACTGGGA TGAGGTGCTG GAGCTGCGGG AGATGCCAGG TTCCCCATCC 
CCTCTGCGAG TGGAACTCTG GGACTGGGAC ATGGTTGGCA AGAATGACTT TTTGGGAATG 
GTGGAGTTCC CCCCACAGGT CCTGCAACAC AACCCGCCCA ATGGCTGGTT CCGCCTCCTG 
CCCTTTCCCA GAGCAGAGGA GGATTCTGGC GGGAGCTTGG GTGCTCTGAG GCTGAAGGTG 
CGCCTGACCG AGGACTCTGT CCTGCCCTCA CGGTACTACC AGCCACTTAG GGAGCTGCTC 
ATGGAGTCGG TGCTGGGGCC AGCAGAGGAG GATGCCGCCA GCCCCTTGGC TGTCCTGGAG 
GAGTTGACCT CGGGTGACTG CCGCCAGGAG CTGGCCACCA AGCTGGTGAA GCTCTTTCTG 
GGCCAGGGCC TGACTGGGCC CTTTCTGGAC TATCTCACCC GGCGCGAGGT GGCTCGGACC 
ACTGACCCCA ACACCCTCTT CCGTTCTAAC TCCCTGGCGT CCAAGTCAGT GGAGCAGTTT 
ATGAAGCTCG TGGGCATGCC CTATCTGCAT GAGGTCCTAA GGCCAGTGAT CAACCGCGTC 
TTCGAGGAGA GGAAGTACAT GGAGCTGGAT CCCTGCAAGA TGGACCTGGG CCGCACCCGG 
AGAATCTCCT TCAAGGGTGC ACCCTCCGAG GAACACGTGC GTGAGGCCAG CCTGGGGCTG 
CTGACGGGCT ACCTGGGGCC TATYGTGGAC GCCATTGTGG GCTCCGTGGG GCGCTGCCCA 
TCCGCCATGC GCCTCGCCTT CAAGCAGCTG CGCCAGCGCG TGGAGGAGCG TTTTCCCCAG 
GCGGAGCATG AGGACGTGAA GTACCTGGCC ATAAGCGGCT TTCTCTTCCT GCGATTCTTC 
GCACCTGCCA TCCTCTCTCC AAAGCTGTTT GACCTCCGGG ACCAGCACGC GGATCCCCAG 
ACCAGCCGCT CACTGCTGCT GCTTGCCAAG GCTGTGCAGA GCATTGGAAA TCTGGGCCAG 
CAGCTGGGCC AGGGCAAGGA GCTGTGGATG GCCCCCCTAC ACCCCTTYCT GCTGCAGAGT 
ATTTCCAGGG TGAGAGACTT CCTGGACCAG CTGGTGGAGG TGGATGGGAA GGAGGAGGCT 
GGGGGCCCAG CCAGGGCCCT GGTCCCGCCC TCAATGACTG TCCGAGAAGG CTACCTACTC 
AAGCGCAAGG AGGAGCCTGC CGGCCTGGCC ACACGCTTTG CCTTCAAGAA GCGCTACTTC 
CGGCTCAGCG GGGAGATGCT GTCCTACTCC AAGAGTCCTG AGTGGCAGRT GCGCTCGTCC 
ATCCCCGTGT CGCACATCCG AGCCGTGGAG CGCGTGGACG AGGGCGCCTT CCAGCTGCCG 
CACGTGATGC AGGTGGTGAC GCAGGACGGC GCGGGGGCGC CGCACACCAC CTACCTCCAG 
TGCAAGAACG TGAACGAGCT CAACCAATGG CTGTCGGCCC TGCGCAAGGC CAGCGCCCCC 
AACCCGGACA AGCTGGCCTC CTRCCACCCC GGTGCCTTCC GCAGCGGGCA CTGGACCTGC 
TGCCTCCAGG CCGAGCGCTC AGCTTCCGGT TGCAGCCGCA CGCACTCGGC CGTCACCCTG 
GGGGACTGGA GTGACCCGCT GGATCCCGAT GCTGAGACCC AGATGGTGTA TCGGCAGCTG 
CTTCTGGGGM GGGACCGGCT CAGGATGAAA TTCCTTGAGG ATTCCAACAT GGATACAACT 
CTGGAGGCAG CCACAGAGCA GGGCTCCAGT GCCATGGAAG GGGCCTGTAC TGATGCCCTG 
GCTCGGCAGA GGGAGGCAGC TGCCCGCCTG CTGAAGGTGC TCACAGACCT GGACCAAGCC 
CACGAGGAGT TCCAGCAGCA GGAACAGGGG AAGGTGGTTT CGGGCCCCCT GAGGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019833219.1
CDS	84..2423
Translation	MDSLACWTATIWRSLSPFWGEEYTVnullLPLDFHHLSFYVLDEDTVGQDDIIGKISLSREAITADPRGIDSWINLSRVDPDSEVQGEICLSVQTLEDVRGRCLHCHVLQARDLAPRDISGTSDPFARVFWGSQSLETSTIKKTRFPHWDEVLELREMPGSPSPLRVELWDWDMVGKNDFLGMVEFPPQVLQHNPPNGWFRLLPFPRAEEDSGGSLGALRLKVRLTEDSVLPSRYYQPLRELLMESVLGPAEEDAASPLAVLEELTSGDCRQELATKLVKLFLGQGLTGPFLDYLTRREVARTTDPNTLFRSNSLASKSVEQFMKLVGMPYLHEVLRPVINRVFEERKYMELDPCKMDLGRTRRISFKGAPSEEHVREASLGLLTGYLGPnullVDAIVGSVGRCPSAMRLAFKQLRQRVEERFPQAEHEDVKYLAISGFLFLRFFAPAILSPKLFDLRDQHADPQTSRSLLLLAKAVQSIGNLGQQLGQGKELWMAPLHPnullLLQSISRVRDFLDQLVEVDGKEEAGGPARALVPPSMTVREGYLLKRKEEPAGLATRFAFKKRYFRLSGEMLSYSKSPEWQnullRSSIPVSHIRAVERVDEGAFQLPHVMQVVTQDGAGAPHTTYLQCKNVNELNQWLSALRKASAPNPDKLASnullHPGAFRSGHWTCCLQAERSASGCSRTHSAVTLGDWSDPLDPDAETQMVYRQLLLGnullDRLRMKFLEDSNMDTTLEAATEQGSSAMEGACTDALARQREAAARLLKVLTDLDQAHEEFQQQEQGKVVSGPLRP

Target ORF information:

RefSeq Version	XM_019977660.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus RAS protein activator like 1 (RASAL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019977660.1

ORF Insert Sequence:

ATGGACTCAC TGGCCTGTTG GACAGCAACC ATCTGGCGGA GCCTGAGCCC CTTCTGGGGG 
GAGGAGTACA CCGTGMACTT GCCCCTGGAT TTCCACCATC TGTCCTTCTA CGTGCTGGAC 
GAGGACACAG TGGGGCAAGA TGACATCATA GGCAAGATCT CGCTGAGCAG GGAGGCCATT 
ACAGCGGACC CCCGAGGAAT CGACAGCTGG ATCAACCTGA GCCGCGTGGA CCCGGACTCG 
GAGGTGCAGG GTGAGATCTG CCTGTCCGTG CAGACGCTGG AGGACGTGCG GGGCCGTTGT 
CTTCACTGTC ACGTGCTCCA GGCTAGGGAC CTGGCCCCCC GAGACATCTC CGGCACGTCT 
GACCCATTTG CGCGTGTGTT TTGGGGCAGC CAGAGCTTGG AGACTTCGAC CATCAAGAAG 
ACTCGCTTCC CACACTGGGA TGAGGTGCTG GAGCTGCGGG AGATGCCAGG TTCCCCATCC 
CCTCTGCGAG TGGAACTCTG GGACTGGGAC ATGGTTGGCA AGAATGACTT TTTGGGAATG 
GTGGAGTTCC CCCCACAGGT CCTGCAACAC AACCCGCCCA ATGGCTGGTT CCGCCTCCTG 
CCCTTTCCCA GAGCAGAGGA GGATTCTGGC GGGAGCTTGG GTGCTCTGAG GCTGAAGGTG 
CGCCTGACCG AGGACTCTGT CCTGCCCTCA CGGTACTACC AGCCACTTAG GGAGCTGCTC 
ATGGAGTCGG TGCTGGGGCC AGCAGAGGAG GATGCCGCCA GCCCCTTGGC TGTCCTGGAG 
GAGTTGACCT CGGGTGACTG CCGCCAGGAG CTGGCCACCA AGCTGGTGAA GCTCTTTCTG 
GGCCAGGGCC TGACTGGGCC CTTTCTGGAC TATCTCACCC GGCGCGAGGT GGCTCGGACC 
ACTGACCCCA ACACCCTCTT CCGTTCTAAC TCCCTGGCGT CCAAGTCAGT GGAGCAGTTT 
ATGAAGCTCG TGGGCATGCC CTATCTGCAT GAGGTCCTAA GGCCAGTGAT CAACCGCGTC 
TTCGAGGAGA GGAAGTACAT GGAGCTGGAT CCCTGCAAGA TGGACCTGGG CCGCACCCGG 
AGAATCTCCT TCAAGGGTGC ACCCTCCGAG GAACACGTGC GTGAGGCCAG CCTGGGGCTG 
CTGACGGGCT ACCTGGGGCC TATYGTGGAC GCCATTGTGG GCTCCGTGGG GCGCTGCCCA 
TCCGCCATGC GCCTCGCCTT CAAGCAGCTG CGCCAGCGCG TGGAGGAGCG TTTTCCCCAG 
GCGGAGCATG AGGACGTGAA GTACCTGGCC ATAAGCGGCT TTCTCTTCCT GCGATTCTTC 
GCACCTGCCA TCCTCTCTCC AAAGCTGTTT GACCTCCGGG ACCAGCACGC GGATCCCCAG 
ACCAGCCGCT CACTGCTGCT GCTTGCCAAG GCTGTGCAGA GCATTGGAAA TCTGGGCCAG 
CAGCTGGGCC AGGGCAAGGA GCTGTGGATG GCCCCCCTAC ACCCCTTYCT GCTGCAGAGT 
ATTTCCAGGG TGAGAGACTT CCTGGACCAG CTGGTGGAGG TGGATGGGAA GGAGGAGGCT 
GGGGGCCCAG CCAGGGCCCT GGTCCCGCCC TCAATGACTG TCCGAGAAGG CTACCTACTC 
AAGCGCAAGG AGGAGCCTGC CGGCCTGGCC ACACGCTTTG CCTTCAAGAA GCGCTACTTC 
CGGCTCAGCG GGGAGATGCT GTCCTACTCC AAGAGTCCTG AGTGGCAGRT GCGCTCGTCC 
ATCCCCGTGT CGCACATCCG AGCCGTGGAG CGCGTGGACG AGGGCGCCTT CCAGCTGCCG 
CACGTGATGC AGGTGGTGAC GCAGGACGGC GCGGGGGCGC CGCACACCAC CTACCTCCAG 
TGCAAGAACG TGAACGAGCT CAACCAATGG CTGTCGGCCC TGCGCAAGGC CAGCGCCCCC 
AACCCGGACA AGCTGGCCTC CTRCCACCCC GGTGCCTTCC GCAGCGGGCA CTGGACCTGC 
TGCCTCCAGG CCGAGCGCTC AGCTTCCGGT TGCAGCCGCA CGCACTCGGC CGTCACCCTG 
GGGGACTGGA GTGACCCGCT GGATCCCGAT GCTGAGACCC AGATGGTGTA TCGGCAGCTG 
CTTCTGGGGM GGGACCGGCT CAGGATGAAA TTCCTTGAGG ATTCCAACAT GGATACAACT 
CTGGAGGCAG CCACAGAGCA GGGCTCCAGT GCCATGGAAG GGGCCTGTAC TGATGCCCTG 
GCTCGGCAGA GGGAGGCAGC TGCCCGCCTG CTGAAGGTGC TCACAGACCT GGACCAAGCC 
CACGAGGAGT TCCAGCAGCA GGAACAGGGG AAGGTGGTTT CGGGCCCCCT GAGGCCCTAA