NEFM cDNA ORF clone, Bos indicus(Bos taurus indicus)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEFM
Entrez Gene ID	109563141
Full Name	neurofilament medium
Gene Type	protein-coding
Organism	Bos indicus(Bos taurus indicus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019966373.1	XP_019821932.1	neurofilament medium polypeptide

Pathways from BioSystems
Gene Ontology

The following NEFM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEFM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo53830	XM_019966373.1 Latest version!	Bos indicus neurofilament medium (NEFM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEFM ( XM_019966373.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019821932.1 Bos indicus neurofilament medium (NEFM), mRNA.

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CloneID	OBo53830
Clone ID Related Accession (Same CDS sequence)	XM_019966373.1
Accession Version	XM_019966373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	neurofilament medium polypeptide
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032657.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACA CGTTGGACTC GCTGGGCAAC CCGTCCGCCT ACCGGCGGGT GACCGAAACC 
CGCTCGAGCT TCAGCCGCAT CAGCGGCTCC CCGTCCAGCG GCTTCCGCTC GCAGTCGTGG 
TCCCGCGGCT CGCCCAGCAC CGTGTCCTCC TCCTACAAGC GCAGCGCGCT GGCCCCGCGC 
CTCACCTACA GCTCCGCCAT GCTCAGCTCG GCCGAGAGCA GCCTCGACTT CAGCCAGTCC 
TCGTCTCTGC TTAACGGCGG TTCTGGGCCT GGAGGCGACT ACAAGCTGTC CCGCTCCAAC 
GAGAAGGAGC AGCTGCAGGG GCTGAACGAC CGCTTCGCGG GCTACATCGA GAAGGTCCAC 
TACCTGGAGC AGCAGAACAA GGAGATCGAG GCAGAGATCC AGGCGCTGCG GCAGAAGCAG 
GCCTCGCACG NNNNNNNNNN NGACGCGTAC GACCAGGAAA TCCGCGAGCT ACGCGCCACC 
CTGGAGATGG TGAACCATGA GAAGGCTCAG GTACAGCTGG ACTCGGACCA CCTGGAAGAG 
GATATCCACC GGCTCAAGGA GCGCTTCGAG GAGGAGGCAC GGCTGCGCGA CGACACCGAG 
GCGGCTATCC GCGCGCTGCG CAAAGATATC GAGGAGTCGT CGCTGGTCAA GGTGGAGCTG 
GACAAGAAGG TGCAGTCGCT GCAGGATGAG GTGGCCTTCC TGCGGAGCAA TCACGAGGAG 
GAGGTGGCCG ACCTGCTGGC CCAGATCCAA GCGTCACACA TCACCGTGGA GCGCAAAGAC 
TACCTGAAGA CGGACATCTC GACGGCGCTG AAAGAGATCC GCTCCCAGCT CGAGAGTCAC 
TCCGACCAGA ACATGCACCA GGCCGAAGAG TGGTTTAAGT GCCGCTACGC CAAGCTCACC 
GAGGCGGCCG AGCAGAACAA GGAAGCCATC CGCTCCGCCA AGGAAGAGAT CGCCGAGTAC 
CGGCGCCAGC TGCAGTCCAA GAGCATCGAG CTCGAGTCAG TGCGCGGCAC CAAGGAGTCC 
CTGGAGCGGC AGCTCAGCGA CATCGAGGAG CGCCACAACC ACGACCTTAG CAGCTACCAG 
GACACCATCC AGCAGCTGGA AAATGAGCTT CGGGGCACAA AGTGGGAAAT GGCTCGTCAT 
CTGCGAGAAT ACCAGGATCT CCTCAACGTC AAGATGGCTC TGGATATTGA GATCGCGGCG 
TACAGGAAAC TCCTGGAGGG TGAAGAGACC AGATTTAGCA CATTTGCGNN AAGCATCACT 
GGGCCACTGT ATACACACCG ACAGCCCTCC ATCGCCATAT CCAGTAAGAT TCAGAAAACC 
AAGGTAGAGG CTCCCAAGCT AAAGGTCCAA CACAAATTTG TTGAGGAGAT TATAGAGGAA 
ACCAAGGTGG AAGATGAGAA ATCAGAAATG GAAGAAGCCC TGACGGCCAT TACCGAGGAA 
TTGGCCGTTT CCGTGAAAGA GGAGGTCAAG GAAGAGGAGG CTGAAGAAAA GGAGGAGAAA 
GAAGAAGCCG AAGAAGAAGT TGTTGCTGCC AAAAAGTCTC CAGTGAAAGC TACTGCACCT 
GAACTTAAAG AAGAGGAAGG AGAAAAGGAG GAGGAAGAGG GCCAAGAGGA AGAGGAAGAG 
GAAGAAGAGG CTGCTAAGTC AGACCAAGCC GAGGAAGGAG GATCTGAGAA GGAAGTTTCT 
AGTGAAAAAG AGGAAGGTGA GCAAGAAGAG GAAGGAGAAA CAGAGGCTGA GGGGGAAGGA 
GAGGAAGCCG CTGCCGAAGC TAAGGAGGAA AAGAAAATGG AGGAAAAGGC TGAAGAAGTG 
GCTCCAAAGG AGGAGCTGGC GGCAGAAGCC AAGGTGGAGA AGCCAGAGAA AGCCAAGTCC 
CCAGTGGCCA AGTCCCCAAC AACAAAGTCC CCAACGGCCA AGTCCCCAAC GGCCAAGTCC 
CCAGAGGCAA AGTCCCCAAC AGCAAAATCC CCGACGGCCA AGTCCCCAGT GGCCAAGTCC 
CCGACGGCCA AGTCCCCGAC GGCCAAGTCC CCAGAGGCAA AGTCCCCAGA GGCAAAGTCC 
CCAACAGCAA AATCCCCGAC GGCCAAGTCC CCAGCAGCAA AGTCCCCAGC GGCAAAATCA 
CCTGTGGAGG AAGTGAAACC CAAAGCAGAA GCTGGAGCTG AGAAAGGAGA ACAGAAGGAG 
AAGGTGGAGG AAGAAAAGAA AGAAGCAAAG GAATCTCCCA AGGAAGAGAA GGCAGAGAAA 
AAGGAGGAGA AGGCAAAGGA TGTGCCAGAG AAGAAGAAGG CTGAATCCCC AGTGAAGGCT 
GAGTCCCCAG TGAAGGAGGA GGTGCCTGCC AAGCCAGTAA AGGTGAGCCC AGAGAAGGAA 
GCCAAAGAGG AGGAGAAGCC ACAGGAGAAA GAGAAGGAGA AGGAGAAAGT GGAAGAGGTG 
GGAGGGAAGG AGGAGGGAGG TTTGAAGGAA TCCAGGAAGG AAGACATAGC CATCAATGGG 
GAGGTAGAAG GGAAGGAGGA AGAACAGGAA ACTAAGGAGA AAGGCAGTGG GGGAGAAGAG 
GAGAAAGGAG TCGTCACCAA CGGGCTAGAC GTGAGCCCAG GGGATGAAAA GAAGGGCGGT 
GATAAAAGTG AGGAGAAAGT GGTGGTAACC AAAATGGTGG AAAAAATCAC CAGTGAGGGG 
GGAGATGGTG CTACCAAGTA TATCACCAAA TCTGTAACCG TCACTCAAAA GGTCGAAGAG 
CATGAAGAGA CCTTTGAGGA GAAACTAGTG TCTACTAAAA AGGTAGAGAA AGTCACTTCA 
CACGCCATAG TAAAGGAAGT CACCCAGAGT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019821932.1
CDS	45..2840
Translation	MSYTLDSLGNPSAYRRVTETRSSFSRISGSPSSGFRSQSWSRGSPSTVSSSYKRSALAPRLTYSSAMLSSAESSLDFSQSSSLLNGGSGPGGDYKLSRSNEKEQLQGLNDRFAGYIEKVHYLEQQNKEIEAEIQALRQKQASHnullnullnullnullDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEESSLVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLESHSDQNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERHNHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFAnullSITGPLYTHRQPSIAISSKIQKTKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEEALTAITEELAVSVKEEVKEEEAEEKEEKEEAEEEVVAAKKSPVKATAPELKEEEGEKEEEEGQEEEEEEEEAAKSDQAEEGGSEKEVSSEKEEGEQEEEGETEAEGEGEEAAAEAKEEKKMEEKAEEVAPKEELAAEAKVEKPEKAKSPVAKSPTTKSPTAKSPTAKSPEAKSPTAKSPTAKSPVAKSPTAKSPTAKSPEAKSPEAKSPTAKSPTAKSPAAKSPAAKSPVEEVKPKAEAGAEKGEQKEKVEEEKKEAKESPKEEKAEKKEEKAKDVPEKKKAESPVKAESPVKEEVPAKPVKVSPEKEAKEEEKPQEKEKEKEKVEEVGGKEEGGLKESRKEDIAINGEVEGKEEEQETKEKGSGGEEEKGVVTNGLDVSPGDEKKGGDKSEEKVVVTKMVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQSD

Target ORF information:

RefSeq Version	XM_019966373.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus neurofilament medium (NEFM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019966373.1

ORF Insert Sequence:

ATGAGCTACA CGTTGGACTC GCTGGGCAAC CCGTCCGCCT ACCGGCGGGT GACCGAAACC 
CGCTCGAGCT TCAGCCGCAT CAGCGGCTCC CCGTCCAGCG GCTTCCGCTC GCAGTCGTGG 
TCCCGCGGCT CGCCCAGCAC CGTGTCCTCC TCCTACAAGC GCAGCGCGCT GGCCCCGCGC 
CTCACCTACA GCTCCGCCAT GCTCAGCTCG GCCGAGAGCA GCCTCGACTT CAGCCAGTCC 
TCGTCTCTGC TTAACGGCGG TTCTGGGCCT GGAGGCGACT ACAAGCTGTC CCGCTCCAAC 
GAGAAGGAGC AGCTGCAGGG GCTGAACGAC CGCTTCGCGG GCTACATCGA GAAGGTCCAC 
TACCTGGAGC AGCAGAACAA GGAGATCGAG GCAGAGATCC AGGCGCTGCG GCAGAAGCAG 
GCCTCGCACG NNNNNNNNNN NGACGCGTAC GACCAGGAAA TCCGCGAGCT ACGCGCCACC 
CTGGAGATGG TGAACCATGA GAAGGCTCAG GTACAGCTGG ACTCGGACCA CCTGGAAGAG 
GATATCCACC GGCTCAAGGA GCGCTTCGAG GAGGAGGCAC GGCTGCGCGA CGACACCGAG 
GCGGCTATCC GCGCGCTGCG CAAAGATATC GAGGAGTCGT CGCTGGTCAA GGTGGAGCTG 
GACAAGAAGG TGCAGTCGCT GCAGGATGAG GTGGCCTTCC TGCGGAGCAA TCACGAGGAG 
GAGGTGGCCG ACCTGCTGGC CCAGATCCAA GCGTCACACA TCACCGTGGA GCGCAAAGAC 
TACCTGAAGA CGGACATCTC GACGGCGCTG AAAGAGATCC GCTCCCAGCT CGAGAGTCAC 
TCCGACCAGA ACATGCACCA GGCCGAAGAG TGGTTTAAGT GCCGCTACGC CAAGCTCACC 
GAGGCGGCCG AGCAGAACAA GGAAGCCATC CGCTCCGCCA AGGAAGAGAT CGCCGAGTAC 
CGGCGCCAGC TGCAGTCCAA GAGCATCGAG CTCGAGTCAG TGCGCGGCAC CAAGGAGTCC 
CTGGAGCGGC AGCTCAGCGA CATCGAGGAG CGCCACAACC ACGACCTTAG CAGCTACCAG 
GACACCATCC AGCAGCTGGA AAATGAGCTT CGGGGCACAA AGTGGGAAAT GGCTCGTCAT 
CTGCGAGAAT ACCAGGATCT CCTCAACGTC AAGATGGCTC TGGATATTGA GATCGCGGCG 
TACAGGAAAC TCCTGGAGGG TGAAGAGACC AGATTTAGCA CATTTGCGNN AAGCATCACT 
GGGCCACTGT ATACACACCG ACAGCCCTCC ATCGCCATAT CCAGTAAGAT TCAGAAAACC 
AAGGTAGAGG CTCCCAAGCT AAAGGTCCAA CACAAATTTG TTGAGGAGAT TATAGAGGAA 
ACCAAGGTGG AAGATGAGAA ATCAGAAATG GAAGAAGCCC TGACGGCCAT TACCGAGGAA 
TTGGCCGTTT CCGTGAAAGA GGAGGTCAAG GAAGAGGAGG CTGAAGAAAA GGAGGAGAAA 
GAAGAAGCCG AAGAAGAAGT TGTTGCTGCC AAAAAGTCTC CAGTGAAAGC TACTGCACCT 
GAACTTAAAG AAGAGGAAGG AGAAAAGGAG GAGGAAGAGG GCCAAGAGGA AGAGGAAGAG 
GAAGAAGAGG CTGCTAAGTC AGACCAAGCC GAGGAAGGAG GATCTGAGAA GGAAGTTTCT 
AGTGAAAAAG AGGAAGGTGA GCAAGAAGAG GAAGGAGAAA CAGAGGCTGA GGGGGAAGGA 
GAGGAAGCCG CTGCCGAAGC TAAGGAGGAA AAGAAAATGG AGGAAAAGGC TGAAGAAGTG 
GCTCCAAAGG AGGAGCTGGC GGCAGAAGCC AAGGTGGAGA AGCCAGAGAA AGCCAAGTCC 
CCAGTGGCCA AGTCCCCAAC AACAAAGTCC CCAACGGCCA AGTCCCCAAC GGCCAAGTCC 
CCAGAGGCAA AGTCCCCAAC AGCAAAATCC CCGACGGCCA AGTCCCCAGT GGCCAAGTCC 
CCGACGGCCA AGTCCCCGAC GGCCAAGTCC CCAGAGGCAA AGTCCCCAGA GGCAAAGTCC 
CCAACAGCAA AATCCCCGAC GGCCAAGTCC CCAGCAGCAA AGTCCCCAGC GGCAAAATCA 
CCTGTGGAGG AAGTGAAACC CAAAGCAGAA GCTGGAGCTG AGAAAGGAGA ACAGAAGGAG 
AAGGTGGAGG AAGAAAAGAA AGAAGCAAAG GAATCTCCCA AGGAAGAGAA GGCAGAGAAA 
AAGGAGGAGA AGGCAAAGGA TGTGCCAGAG AAGAAGAAGG CTGAATCCCC AGTGAAGGCT 
GAGTCCCCAG TGAAGGAGGA GGTGCCTGCC AAGCCAGTAA AGGTGAGCCC AGAGAAGGAA 
GCCAAAGAGG AGGAGAAGCC ACAGGAGAAA GAGAAGGAGA AGGAGAAAGT GGAAGAGGTG 
GGAGGGAAGG AGGAGGGAGG TTTGAAGGAA TCCAGGAAGG AAGACATAGC CATCAATGGG 
GAGGTAGAAG GGAAGGAGGA AGAACAGGAA ACTAAGGAGA AAGGCAGTGG GGGAGAAGAG 
GAGAAAGGAG TCGTCACCAA CGGGCTAGAC GTGAGCCCAG GGGATGAAAA GAAGGGCGGT 
GATAAAAGTG AGGAGAAAGT GGTGGTAACC AAAATGGTGG AAAAAATCAC CAGTGAGGGG 
GGAGATGGTG CTACCAAGTA TATCACCAAA TCTGTAACCG TCACTCAAAA GGTCGAAGAG 
CATGAAGAGA CCTTTGAGGA GAAACTAGTG TCTACTAAAA AGGTAGAGAA AGTCACTTCA 
CACGCCATAG TAAAGGAAGT CACCCAGAGT GACTAA