Atg5 cDNA ORF clone, Bombyx mori(domestic silkworm)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atg5
Entrez Gene ID	100216348
Full Name	autophagy 5-like
Gene Type	protein-coding
Organism	Bombyx mori(domestic silkworm)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001142487.1	NP_001135959.1	autophagy 5-like

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Upregulation of Atg5 and AIF gene expression in synchronization with programmed cellular death events in integumental epithelium of Bombyx mori induced by a dipteran parasitoid infection.
Anitha J, Pradeep AR, Sivaprasad V
Bulletin of entomological research104(6)794-800(2014 Dec)

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
Ou J, Deng HM, Zheng SC, Huang LH, Feng QL, Liu L
BMC genomics15820(2014 Sep)

Systematic cloning and analysis of autophagy-related genes from the silkworm Bombyx mori.
Zhang X, Hu ZY, Li WF, Li QR, Deng XJ, Yang WY, Cao Y, Zhou CZ
BMC molecular biology1050(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Activation of Atg5, AIF and caspase genes in close association with different cell death events revealed the synchronized differential expression of apoptosis-associated genes in response to macroparasitism.
Title: Upregulation of Atg5 and AIF gene expression in synchronization with programmed cellular death events in integumental epithelium of Bombyx mori induced by a dipteran parasitoid infection.

The following Atg5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atg5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBg13495	NM_001142487.1 Latest version!	Bombyx mori autophagy 5-like (Atg5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atg5 ( NM_001142487.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> NP_001135959.1 Bombyx mori autophagy 5-like (Atg5), mRNA.

Price & Availability↑

CloneID	OBg13495
Clone ID Related Accession (Same CDS sequence)	NM_001142487.1
Accession Version	NM_001142487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-24
Organism	Bombyx mori(domestic silkworm)
Product	autophagy 5-like
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FJ418152.1. ##Evidence-Data-START## Transcript exon combination :: FJ418152.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00016912, SAMD00016913 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAACG ACAGGGAGGT ACTTCGAGAA ATATGGGATG GAAAACTACC GATATGTTTT 
CATCTTGCTC AAGAAGAAAT AATGGAAATT CAACAACCTG ATCCATTTTA CGTTATGGTG 
CCTCGACTTA GCTACTTTCC TCTTGTCATC GATAAGATGA AACGTCACTT TCTCCGATTC 
ATATCACAGG AGAATTCTGA TAGTGAAATG TGGTTGGACT TTAATGGGCA ACCCTTGAAA 
TGGCACTATC CCATAGGATT TTTGTACGAT TTGTATTGTG GTAATGATCC TCAACTACCC 
TGGACTTTGA CAGTACACTT CACAAAGTTC CCTGAAGACA TTCTTCTTCA TTGCCCAAAC 
AAAGATGTAG TAGAAGCACA TTATATGTCT ACAGTGAAAG AGGCTGATGT ACTGAAACAC 
AGAGGACAAG TGATGTCAAC TATGCAAAAA AAGGATCACA ATCAGTTATG GCTTGGATTA 
CAAAATGACA AGTTTGATCA GTTTTGGGCT ATCAACAGAC GTCTTATGGA GTCCCATGGT 
GATAATGATG GATTCAAACA CATACCACTT AGGATTTATA CTGATGATGG TGTATACAAT 
CAACGCCTTG TCTGTCCTAA AAATACTGAT AATAGTAGAA AAACGCTACA ACAGATGATC 
TCCGAGCTAT ATCCAGAAAA ATCAAATGTT AATCTAAGAA CTCATGGTGT AATCATACCT 
CCAGAAACTC CTCTGCAGTG GCTTTCAGAA CATCTCAGTT ACCCTGACAA CTTTTTACAT 
TTGTGTTTAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135959.1
CDS	1..795
Translation	MANDREVLREIWDGKLPICFHLAQEEIMEIQQPDPFYVMVPRLSYFPLVIDKMKRHFLRFISQENSDSEMWLDFNGQPLKWHYPIGFLYDLYCGNDPQLPWTLTVHFTKFPEDILLHCPNKDVVEAHYMSTVKEADVLKHRGQVMSTMQKKDHNQLWLGLQNDKFDQFWAINRRLMESHGDNDGFKHIPLRIYTDDGVYNQRLVCPKNTDNSRKTLQQMISELYPEKSNVNLRTHGVIIPPETPLQWLSEHLSYPDNFLHLCLC

Target ORF information:

RefSeq Version	NM_001142487.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori autophagy 5-like (Atg5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142487.1

ORF Insert Sequence:

ATGGCCAACG ACAGGGAGGT ACTTCGAGAA ATATGGGATG GAAAACTACC GATATGTTTT 
CATCTTGCTC AAGAAGAAAT AATGGAAATT CAACAACCTG ATCCATTTTA CGTTATGGTG 
CCTCGACTTA GCTACTTTCC TCTTGTCATC GATAAGATGA AACGTCACTT TCTCCGATTC 
ATATCACAGG AGAATTCTGA TAGTGAAATG TGGTTGGACT TTAATGGGCA ACCCTTGAAA 
TGGCACTATC CCATAGGATT TTTGTACGAT TTGTATTGTG GTAATGATCC TCAACTACCC 
TGGACTTTGA CAGTACACTT CACAAAGTTC CCTGAAGACA TTCTTCTTCA TTGCCCAAAC 
AAAGATGTAG TAGAAGCACA TTATATGTCT ACAGTGAAAG AGGCTGATGT ACTGAAACAC 
AGAGGACAAG TGATGTCAAC TATGCAAAAA AAGGATCACA ATCAGTTATG GCTTGGATTA 
CAAAATGACA AGTTTGATCA GTTTTGGGCT ATCAACAGAC GTCTTATGGA GTCCCATGGT 
GATAATGATG GATTCAAACA CATACCACTT AGGATTTATA CTGATGATGG TGTATACAAT 
CAACGCCTTG TCTGTCCTAA AAATACTGAT AATAGTAGAA AAACGCTACA ACAGATGATC 
TCCGAGCTAT ATCCAGAAAA ATCAAATGTT AATCTAAGAA CTCATGGTGT AATCATACCT 
CCAGAAACTC CTCTGCAGTG GCTTTCAGAA CATCTCAGTT ACCCTGACAA CTTTTTACAT 
TTGTGTTTAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Upregulation of Atg5 and AIF gene expression in synchronization with programmed cellular death events in integumental epithelium of Bombyx mori induced by a dipteran parasitoid infection.
Bulletin of entomological research104(6)794-800(2014 Dec)
Anitha J,Pradeep AR,Sivaprasad V

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
BMC genomics15820(2014 Sep)
Ou J,Deng HM,Zheng SC,Huang LH,Feng QL,Liu L

Systematic cloning and analysis of autophagy-related genes from the silkworm Bombyx mori.
BMC molecular biology1050(2009 May)
Zhang X,Hu ZY,Li WF,Li QR,Deng XJ,Yang WY,Cao Y,Zhou CZ