LOC100650372 cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100650372
Entrez Gene ID	100650372
Full Name	endothelin-converting enzyme 1
Gene Type	protein-coding
Organism	Bombus terrestris(buff-tailed bumblebee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012314807.2	XP_012170197.2	endothelin-converting enzyme 1

Pathways from BioSystems
Gene Ontology

The following LOC100650372 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100650372 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo61294	XM_012314807.2 Latest version!	Bombus terrestris endothelin-converting enzyme 1 (LOC100650372), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100650372 ( XM_012314807.2 ) cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee) -> XP_012170197.2 Bombus terrestris endothelin-converting enzyme 1 (LOC100650372), mRNA.

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CloneID	OBo61294
Clone ID Related Accession (Same CDS sequence)	XM_012314807.2
Accession Version	XM_012314807.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Bombus terrestris(buff-tailed bumblebee)
Product	endothelin-converting enzyme 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_015773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 25, 2017 this sequence version replaced XM_012314807.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus terrestris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATAA AATTCACGAC AGCGGTTTTT CTCGCCTATT GTAAAAAGAG GAAGGCTGGA 
TGTCACATCG GGAGCCTAAC TTTCAAGTTT GGCTTCGATC ACATCGTTAA AATGTTACGT 
CTACTGATAC TAGTGTCCCT GTGCGTCGTT ATAAACTCCG GTCTAAAACT CGATGATCTG 
CTGAAATATC CTCGCAGTTT ACAACGTTTG TTTGAATCTA AGGGTGCTGT TGAAGCGAAG 
AAAGTTGAAC GTGAAGTATG TAAGACCGAA GAATGCAAGC TGATTGCACA ATTGATCAAG 
GGCAGTATGG ACGTGTCCGT AGACCCGTGT GACGATTTTT ACGAGTATGC TTGTGGCAAC 
TGGTCGAAAA TCAATCCTCT TCCTGAAAAT AAGACCTCCT GGAGCTTATG GAATTTGGTA 
GCCGAGAAAG TCAAGCAACA AGCTAAAAAT ATAATTTCGG TTAAACCAAA ACCTGACGAT 
CTTTACGCGG TGAGACTTTC TAAGAAGTGG TATCACAGCT GTATGGATAC AGATGCCATA 
GAAAAAAGAG GAGTGGAACC ACTATTGTCA ACCCTATGGA GACACGGAGG ATGGCCACTT 
ATCATGGAAG AGGGAGAGTG GGATGAGGAT ATATACAACT GGCAAATAGT CGATGACCAA 
TTTGCTCGTA TATCAGGATT TAACGCATTT CACGATTTAC GTTACGTCCC ACAATCACCT 
GAAAGCAACC ATACGATAAT TATTATGTTA CCTCATTTAC CGCAGGGTGC ATATGGGCTT 
ATTACAGTGG AGAAAATATT GAATTTAGAC AGCAGCGATG AAAATAACGA AAGTGGAGAA 
GGTAGCCAGG AAAAAGGAAG CAAGGAAAGA GCAGAACAAA AGCAGCATGA AGAGAATGAC 
GAAGAAGATG AAGAAGAGAA TGAATCAGAG GACGACTGGA ACACTGTAGA AGAAGATGAA 
GAAGGAGAGA CGAGAAAGAA GAAAATGATC GCTAGAAAAT ACCAGAGAAA ATTAGGTCAC 
GCTAAGAAGG GGCATAATAA GAAAAAAGTT ATGCATAATA TTATTGGTAA AAAGAAAAGG 
ACGAGAAGAA CTAAAAGGAA TATTATAGGC GAGAAACATC TTCATAAACT ATTGCGTAAA 
CTAAAGGCTA ATAAATATAA TATCAACAAT AAGGTCAACA ATAATGGACA TTTGTCACGC 
AATAAGTCGA TTACAAAGAA ACATAGTATG AAAGATAAAA CGATAAGGAA AAATTTACGA 
AACCACTTAG GTCGCAATAA ACATAATAAC AAGAAGAAGA AGGTGAAAGA GAACAAGAAA 
GAAGGGGAAG TACTACGCAA GAGGAAAATA CACAGAAGGA AGATTACCAA GCAATTAGGG 
CATAGCAGAA GGATGCATAA TAAGAACAAA GCATTGGGAA AACATACCTA CGAGGAGAAG 
ATGAAGAAGA AATTGCAGAG GACTGGAACG AAAGCAAAGG TTCATGCCGG TGAAAATCAT 
AAGCACCAGA AAATTACAGG CGCCACAGCG CATCACGTCG CAAACAAGAT GAACGTTTCG 
AAACATAAGC AAATATCCGA CTATACTGAA GATACATATA ACAGTGTCGA TACCAACGAT 
GACGATGATA ATAATAACAA CGATGAATAT AACGAATATA GTGCCGAAGC TAACGAAGAC 
GATAACGAGG AAGAGAACTA CGACGACGAC GATGATAACG ATGAGAATAA CGAAGATGAC 
GACGAAGATA ACTACGAGGA TGACGAAAAT GACGACGAAG ATAACTACGA GGATGACGAA 
GATGACGATA ATGATGACAA TGATAATAAT TACGAGCATG ATGAAGAAGA AGTTGAAGAG 
CAAAGACAAA CATATCGAGA TTACATATTA AATGTATCTT TAATACTTTC TAGGGAAAGA 
GGAATTGAAA TACCGGAGGA ACAAATGGTG AAAGATGTCG AAGATCTCGT AAAATTTATC 
GTTAATTTGA CAGAAATCGT ACATGCCGTT GAGGATGACG ATGAACAAAT CGAGAATATA 
ACGCTTAAAG AATTTCAAGA ATACTACGAA GATTTAGGTC CTATTTCGCG TACTAACAGA 
ATAAATTGGA TAAGAAAAAT ACAAAAAATA TTTTCCGAGG CTGGATTGGA GTTTGATAAC 
GACGTAGAAG TTTCTGTATC GTATCCCAAA TATATGGAAG AACTTCATAC CTTGCTCGCA 
AAAACTCCAG AAAGAGTACT TGTTAACTAT ATTCATTGGC TCTTCATTAG TAGGATAATG 
ATAGCTGGAC CATCAGAACT AACAGAATTG TTCGAACAAT GGACCGCAGA CTGGTTCTAT 
TCCACAAGAG AGCAGATGTG CCTGGAAATT GCGCCACTGG GTAAAGTAGC AGGATACGAA 
TACATTAAAC AATATCTGCC AGAAAACGTA GCAAAAATGG CCAAAGACAT GATCGACGAC 
ATACAAAAGG AAATCGAATA TCGCATCGAA GCGTCGAACT GGATGGACGA TGATACTAAA 
CATTTTGTTT TGGACAAATT AGTGCACATG AAGAACTGGG TTGGCTATCC AAGTTGGCTG 
CAAAACTCTA CGCTCGTAAA ACGCTACTTC CGAGGGTTTA CAGTCGGTAC TTCATTCTAC 
GAGAACTTCC TCAACTATGC GAGATATTCT AGATGGAAAA GCTTACGACG AAATCTAGAT 
AATAGCGAAA TTTACGAGAT GGATCCCCTG GAATTGAATG CTTTCTTCAT ACCAAACGAT 
AATTCTATAT CAATCACGGC GGCAGATTTG CAAAATCCAT TGTTTGCTCA AAATCGACCT 
TGGTATGTGA ATTTCGGTAT TATTGGTCTC GTCATGGGTC ATGAAGTAAA TCACGGATTC 
GATGATGAAG GTCATCTTTA TGACGAAGGA GGAAATCAAG TGGAATGGCT AACTGCAATG 
GCAGACGCTT ACGATAAGAG AGCAATATGT TTCGTGAACC AATACGATGG TTATAGTATC 
ATCAAAGGAG AAAATTACAC GATCGAGAAT TATGGTAACA GAACCGCAGG TGAAAATATT 
GCGGATTCGA TGGGCCTGCA TGCTGTGTTC GGTGCTTATA AACGAAGGGA ACGAGAATGT 
GTAAATGCGG ACCCTGCTCT ACCAGGACTA GAACAATTTA GCAACGACCA ACTTTTCTTC 
TTATCATTTG CAAACTTATG GTGCGAAACA GCAGACAAAG AAGCATTAAT ATCCCAGAGC 
AAATACGACG TGCATAGTAC GGCACGTTTA AGAGTTATTG GTTCGGTGTC AAATTCAGAA 
GATTTCGCCA AAGCTTTCAA CTGTCCAGTA GGTAGTCCTA TGAATCCTGA AAACAAATGC 
AACATATGGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012170197.2
CDS	4..3378
Translation	MSIKFTTAVFLAYCKKRKAGCHIGSLTFKFGFDHIVKMLRLLILVSLCVVINSGLKLDDLLKYPRSLQRLFESKGAVEAKKVEREVCKTEECKLIAQLIKGSMDVSVDPCDDFYEYACGNWSKINPLPENKTSWSLWNLVAEKVKQQAKNIISVKPKPDDLYAVRLSKKWYHSCMDTDAIEKRGVEPLLSTLWRHGGWPLIMEEGEWDEDIYNWQIVDDQFARISGFNAFHDLRYVPQSPESNHTIIIMLPHLPQGAYGLITVEKILNLDSSDENNESGEGSQEKGSKERAEQKQHEENDEEDEEENESEDDWNTVEEDEEGETRKKKMIARKYQRKLGHAKKGHNKKKVMHNIIGKKKRTRRTKRNIIGEKHLHKLLRKLKANKYNINNKVNNNGHLSRNKSITKKHSMKDKTIRKNLRNHLGRNKHNNKKKKVKENKKEGEVLRKRKIHRRKITKQLGHSRRMHNKNKALGKHTYEEKMKKKLQRTGTKAKVHAGENHKHQKITGATAHHVANKMNVSKHKQISDYTEDTYNSVDTNDDDDNNNNDEYNEYSAEANEDDNEEENYDDDDDNDENNEDDDEDNYEDDENDDEDNYEDDEDDDNDDNDNNYEHDEEEVEEQRQTYRDYILNVSLILSRERGIEIPEEQMVKDVEDLVKFIVNLTEIVHAVEDDDEQIENITLKEFQEYYEDLGPISRTNRINWIRKIQKIFSEAGLEFDNDVEVSVSYPKYMEELHTLLAKTPERVLVNYIHWLFISRIMIAGPSELTELFEQWTADWFYSTREQMCLEIAPLGKVAGYEYIKQYLPENVAKMAKDMIDDIQKEIEYRIEASNWMDDDTKHFVLDKLVHMKNWVGYPSWLQNSTLVKRYFRGFTVGTSFYENFLNYARYSRWKSLRRNLDNSEIYEMDPLELNAFFIPNDNSISITAADLQNPLFAQNRPWYVNFGIIGLVMGHEVNHGFDDEGHLYDEGGNQVEWLTAMADAYDKRAICFVNQYDGYSIIKGENYTIENYGNRTAGENIADSMGLHAVFGAYKRRERECVNADPALPGLEQFSNDQLFFLSFANLWCETADKEALISQSKYDVHSTARLRVIGSVSNSEDFAKAFNCPVGSPMNPENKCNIWE

Target ORF information:

RefSeq Version	XM_012314807.2
Organism	Bombus terrestris(buff-tailed bumblebee)
Definition	Bombus terrestris endothelin-converting enzyme 1 (LOC100650372), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012314807.2

ORF Insert Sequence:

ATGAGTATAA AATTCACGAC AGCGGTTTTT CTCGCCTATT GTAAAAAGAG GAAGGCTGGA 
TGTCACATCG GGAGCCTAAC TTTCAAGTTT GGCTTCGATC ACATCGTTAA AATGTTACGT 
CTACTGATAC TAGTGTCCCT GTGCGTCGTT ATAAACTCCG GTCTAAAACT CGATGATCTG 
CTGAAATATC CTCGCAGTTT ACAACGTTTG TTTGAATCTA AGGGTGCTGT TGAAGCGAAG 
AAAGTTGAAC GTGAAGTATG TAAGACCGAA GAATGCAAGC TGATTGCACA ATTGATCAAG 
GGCAGTATGG ACGTGTCCGT AGACCCGTGT GACGATTTTT ACGAGTATGC TTGTGGCAAC 
TGGTCGAAAA TCAATCCTCT TCCTGAAAAT AAGACCTCCT GGAGCTTATG GAATTTGGTA 
GCCGAGAAAG TCAAGCAACA AGCTAAAAAT ATAATTTCGG TTAAACCAAA ACCTGACGAT 
CTTTACGCGG TGAGACTTTC TAAGAAGTGG TATCACAGCT GTATGGATAC AGATGCCATA 
GAAAAAAGAG GAGTGGAACC ACTATTGTCA ACCCTATGGA GACACGGAGG ATGGCCACTT 
ATCATGGAAG AGGGAGAGTG GGATGAGGAT ATATACAACT GGCAAATAGT CGATGACCAA 
TTTGCTCGTA TATCAGGATT TAACGCATTT CACGATTTAC GTTACGTCCC ACAATCACCT 
GAAAGCAACC ATACGATAAT TATTATGTTA CCTCATTTAC CGCAGGGTGC ATATGGGCTT 
ATTACAGTGG AGAAAATATT GAATTTAGAC AGCAGCGATG AAAATAACGA AAGTGGAGAA 
GGTAGCCAGG AAAAAGGAAG CAAGGAAAGA GCAGAACAAA AGCAGCATGA AGAGAATGAC 
GAAGAAGATG AAGAAGAGAA TGAATCAGAG GACGACTGGA ACACTGTAGA AGAAGATGAA 
GAAGGAGAGA CGAGAAAGAA GAAAATGATC GCTAGAAAAT ACCAGAGAAA ATTAGGTCAC 
GCTAAGAAGG GGCATAATAA GAAAAAAGTT ATGCATAATA TTATTGGTAA AAAGAAAAGG 
ACGAGAAGAA CTAAAAGGAA TATTATAGGC GAGAAACATC TTCATAAACT ATTGCGTAAA 
CTAAAGGCTA ATAAATATAA TATCAACAAT AAGGTCAACA ATAATGGACA TTTGTCACGC 
AATAAGTCGA TTACAAAGAA ACATAGTATG AAAGATAAAA CGATAAGGAA AAATTTACGA 
AACCACTTAG GTCGCAATAA ACATAATAAC AAGAAGAAGA AGGTGAAAGA GAACAAGAAA 
GAAGGGGAAG TACTACGCAA GAGGAAAATA CACAGAAGGA AGATTACCAA GCAATTAGGG 
CATAGCAGAA GGATGCATAA TAAGAACAAA GCATTGGGAA AACATACCTA CGAGGAGAAG 
ATGAAGAAGA AATTGCAGAG GACTGGAACG AAAGCAAAGG TTCATGCCGG TGAAAATCAT 
AAGCACCAGA AAATTACAGG CGCCACAGCG CATCACGTCG CAAACAAGAT GAACGTTTCG 
AAACATAAGC AAATATCCGA CTATACTGAA GATACATATA ACAGTGTCGA TACCAACGAT 
GACGATGATA ATAATAACAA CGATGAATAT AACGAATATA GTGCCGAAGC TAACGAAGAC 
GATAACGAGG AAGAGAACTA CGACGACGAC GATGATAACG ATGAGAATAA CGAAGATGAC 
GACGAAGATA ACTACGAGGA TGACGAAAAT GACGACGAAG ATAACTACGA GGATGACGAA 
GATGACGATA ATGATGACAA TGATAATAAT TACGAGCATG ATGAAGAAGA AGTTGAAGAG 
CAAAGACAAA CATATCGAGA TTACATATTA AATGTATCTT TAATACTTTC TAGGGAAAGA 
GGAATTGAAA TACCGGAGGA ACAAATGGTG AAAGATGTCG AAGATCTCGT AAAATTTATC 
GTTAATTTGA CAGAAATCGT ACATGCCGTT GAGGATGACG ATGAACAAAT CGAGAATATA 
ACGCTTAAAG AATTTCAAGA ATACTACGAA GATTTAGGTC CTATTTCGCG TACTAACAGA 
ATAAATTGGA TAAGAAAAAT ACAAAAAATA TTTTCCGAGG CTGGATTGGA GTTTGATAAC 
GACGTAGAAG TTTCTGTATC GTATCCCAAA TATATGGAAG AACTTCATAC CTTGCTCGCA 
AAAACTCCAG AAAGAGTACT TGTTAACTAT ATTCATTGGC TCTTCATTAG TAGGATAATG 
ATAGCTGGAC CATCAGAACT AACAGAATTG TTCGAACAAT GGACCGCAGA CTGGTTCTAT 
TCCACAAGAG AGCAGATGTG CCTGGAAATT GCGCCACTGG GTAAAGTAGC AGGATACGAA 
TACATTAAAC AATATCTGCC AGAAAACGTA GCAAAAATGG CCAAAGACAT GATCGACGAC 
ATACAAAAGG AAATCGAATA TCGCATCGAA GCGTCGAACT GGATGGACGA TGATACTAAA 
CATTTTGTTT TGGACAAATT AGTGCACATG AAGAACTGGG TTGGCTATCC AAGTTGGCTG 
CAAAACTCTA CGCTCGTAAA ACGCTACTTC CGAGGGTTTA CAGTCGGTAC TTCATTCTAC 
GAGAACTTCC TCAACTATGC GAGATATTCT AGATGGAAAA GCTTACGACG AAATCTAGAT 
AATAGCGAAA TTTACGAGAT GGATCCCCTG GAATTGAATG CTTTCTTCAT ACCAAACGAT 
AATTCTATAT CAATCACGGC GGCAGATTTG CAAAATCCAT TGTTTGCTCA AAATCGACCT 
TGGTATGTGA ATTTCGGTAT TATTGGTCTC GTCATGGGTC ATGAAGTAAA TCACGGATTC 
GATGATGAAG GTCATCTTTA TGACGAAGGA GGAAATCAAG TGGAATGGCT AACTGCAATG 
GCAGACGCTT ACGATAAGAG AGCAATATGT TTCGTGAACC AATACGATGG TTATAGTATC 
ATCAAAGGAG AAAATTACAC GATCGAGAAT TATGGTAACA GAACCGCAGG TGAAAATATT 
GCGGATTCGA TGGGCCTGCA TGCTGTGTTC GGTGCTTATA AACGAAGGGA ACGAGAATGT 
GTAAATGCGG ACCCTGCTCT ACCAGGACTA GAACAATTTA GCAACGACCA ACTTTTCTTC 
TTATCATTTG CAAACTTATG GTGCGAAACA GCAGACAAAG AAGCATTAAT ATCCCAGAGC 
AAATACGACG TGCATAGTAC GGCACGTTTA AGAGTTATTG GTTCGGTGTC AAATTCAGAA 
GATTTCGCCA AAGCTTTCAA CTGTCCAGTA GGTAGTCCTA TGAATCCTGA AAACAAATGC 
AACATATGGG AATAA