LOC100749430 cDNA ORF clone, Bombus impatiens(common eastern bumble bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100749430
Entrez Gene ID	100749430
Full Name	probable ATP-dependent RNA helicase DDX47
Gene Type	protein-coding
Organism	Bombus impatiens(common eastern bumble bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003485330.3	XP_003485378.1	probable ATP-dependent RNA helicase DDX47
XM_003485330.2	XP_003485378.1	probable ATP-dependent RNA helicase DDX47

Pathways from BioSystems
Gene Ontology

The following LOC100749430 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100749430 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBo97284	XM_003485330.3 Latest version!	Bombus impatiens probable ATP-dependent RNA helicase DDX47 (LOC100749430), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
OBo97284	XM_003485330.2	Bombus impatiens probable ATP-dependent RNA helicase DDX47 (LOC100749430), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100749430 ( XM_003485330.3 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_003485378.1 Bombus impatiens probable ATP-dependent RNA helicase DDX47 (LOC100749430), mRNA.

Price & Availability↑

CloneID	OBo97284
Clone ID Related Accession (Same CDS sequence)	XM_003485330.3 , XM_003485330.2
Accession Version	XM_003485330.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	probable ATP-dependent RNA helicase DDX47
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176470.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 19, 2018 this sequence version replaced XM_003485330.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAATCCAAC AAAACAACAG GAACAAGAAA ATGTAAAGGA ACTGACATGG 
AAAGATTTGG GGATCGTAGA CACATTGTGT AAAACATGTG AAGATTTAAA ATGGAAATCA 
CCTACAAAAA TTCAATGTGA AGCTATTCCG TTAACCCTTG AAGGTAAAGA TGTAATAGGA 
TTAGCAGAAA CTGGTTCTGG TAAAACTGCA GCCTTTGCTA TTCCTATATT ACAGGCACTG 
TTAGAAAATC CACAAAGATA TTTTGCTTTA ATTTTAACAC CTACCAGAGA ACTAGCCTTC 
CAAATATCAG AACAATTTGA AGCTCTAGGG TCAAGTATTG GAGTAAAATG TGCAGTAATA 
GTAGGTGGAA TGGACATGAT GTCTCAGGCA TTGTTATTGG CAAAAAAGCC ACATATTTTA 
ATTGCCACTC CAGGTAGATT AGTTGATCAC TTGGAAAACA CAAAAGGTTT TAATCTACGT 
TCTTTAAAAT TCTTGGTTAT GGATGAGGCA GATCGAATTT TGAATATGGA TTTTGAAGTT 
GAAGTAGATA AAATTTTAAG GGTAATTCCT CGAGAAAGAA GAACATTGTT GTTTTCTGCA 
ACAATGACTA AAAAGGTTCA GAAATTACAG AGAGCATCTT TGCGAAACCC TGTTAAAGTG 
GAAGTTTCAA CAAAGTACCA AACTGTTGAA AAATTACAAC AATATTATAT TTTTATCCCA 
GTTAAATTCA AGGATGTATA TCTCGTACAT ATCTTAAATG AATTAGCAGG TAACAGTTTT 
ATGATATTTT GTGCGACTTG CAACAATACA GTTAGAACTG CTCTTCTTTT ACGAAATTTG 
GGCTTTACTG CTGTTCCTTT GCATGGACAG ATGTCACAGA ATAAGAGAAT AGCTGCACTT 
ACTAAATTCA AGGCGAAAAA TCGATCTATA CTTATTTCCA CAGATGTTGC TAGCAGGGGT 
CTCGATATTC CTCATGTAGA TATTGTAATA AATTTCGATA TACCTACACA TAGTAAAGAT 
TATATACATA GAGTTGGACG TACTGCACGT GCTGGTCGTT CTGGTCGTTC CATTACGTTT 
GTAACACAGT ATGACGTAGA ATTGTATCAA AGAATAGAGC AATTGATATC AAAACAATTA 
CCATTATATC CGACTGAAGA AGAAGAAGTA ATGGTGCTCC AAGAAAGAGT AGCTGAAGCA 
CAACGTATTG TAAAAATGGA AATGAAAGAT ATCGAGGACA GTAAAAAATT AGGTAAACGA 
AAGAAGCGTC AAGATGACAA TGAGGAGGAT GATACAGAAC AATCTATGGG AGTCAGAAAA 
AGGATAAAAG GCAAAAATAA AGGAAGGGCC AAAAAGGGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003485378.1
CDS	162..1523
Translation	MEESNPTKQQEQENVKELTWKDLGIVDTLCKTCEDLKWKSPTKIQCEAIPLTLEGKDVIGLAETGSGKTAAFAIPILQALLENPQRYFALILTPTRELAFQISEQFEALGSSIGVKCAVIVGGMDMMSQALLLAKKPHILIATPGRLVDHLENTKGFNLRSLKFLVMDEADRILNMDFEVEVDKILRVIPRERRTLLFSATMTKKVQKLQRASLRNPVKVEVSTKYQTVEKLQQYYIFIPVKFKDVYLVHILNELAGNSFMIFCATCNNTVRTALLLRNLGFTAVPLHGQMSQNKRIAALTKFKAKNRSILISTDVASRGLDIPHVDIVINFDIPTHSKDYIHRVGRTARAGRSGRSITFVTQYDVELYQRIEQLISKQLPLYPTEEEEVMVLQERVAEAQRIVKMEMKDIEDSKKLGKRKKRQDDNEEDDTEQSMGVRKRIKGKNKGRAKKG

Target ORF information:

RefSeq Version	XM_003485330.3
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens probable ATP-dependent RNA helicase DDX47 (LOC100749430), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003485330.3

ORF Insert Sequence:

ATGGAAGAAA GTAATCCAAC AAAACAACAG GAACAAGAAA ATGTAAAGGA ACTGACATGG 
AAAGATTTGG GGATCGTAGA CACATTGTGT AAAACATGTG AAGATTTAAA ATGGAAATCA 
CCTACAAAAA TTCAATGTGA AGCTATTCCG TTAACCCTTG AAGGTAAAGA TGTAATAGGA 
TTAGCAGAAA CTGGTTCTGG TAAAACTGCA GCCTTTGCTA TTCCTATATT ACAGGCACTG 
TTAGAAAATC CACAAAGATA TTTTGCTTTA ATTTTAACAC CTACCAGAGA ACTAGCCTTC 
CAAATATCAG AACAATTTGA AGCTCTAGGG TCAAGTATTG GAGTAAAATG TGCAGTAATA 
GTAGGTGGAA TGGACATGAT GTCTCAGGCA TTGTTATTGG CAAAAAAGCC ACATATTTTA 
ATTGCCACTC CAGGTAGATT AGTTGATCAC TTGGAAAACA CAAAAGGTTT TAATCTACGT 
TCTTTAAAAT TCTTGGTTAT GGATGAGGCA GATCGAATTT TGAATATGGA TTTTGAAGTT 
GAAGTAGATA AAATTTTAAG GGTAATTCCT CGAGAAAGAA GAACATTGTT GTTTTCTGCA 
ACAATGACTA AAAAGGTTCA GAAATTACAG AGAGCATCTT TGCGAAACCC TGTTAAAGTG 
GAAGTTTCAA CAAAGTACCA AACTGTTGAA AAATTACAAC AATATTATAT TTTTATCCCA 
GTTAAATTCA AGGATGTATA TCTCGTACAT ATCTTAAATG AATTAGCAGG TAACAGTTTT 
ATGATATTTT GTGCGACTTG CAACAATACA GTTAGAACTG CTCTTCTTTT ACGAAATTTG 
GGCTTTACTG CTGTTCCTTT GCATGGACAG ATGTCACAGA ATAAGAGAAT AGCTGCACTT 
ACTAAATTCA AGGCGAAAAA TCGATCTATA CTTATTTCCA CAGATGTTGC TAGCAGGGGT 
CTCGATATTC CTCATGTAGA TATTGTAATA AATTTCGATA TACCTACACA TAGTAAAGAT 
TATATACATA GAGTTGGACG TACTGCACGT GCTGGTCGTT CTGGTCGTTC CATTACGTTT 
GTAACACAGT ATGACGTAGA ATTGTATCAA AGAATAGAGC AATTGATATC AAAACAATTA 
CCATTATATC CGACTGAAGA AGAAGAAGTA ATGGTGCTCC AAGAAAGAGT AGCTGAAGCA 
CAACGTATTG TAAAAATGGA AATGAAAGAT ATCGAGGACA GTAAAAAATT AGGTAAACGA 
AAGAAGCGTC AAGATGACAA TGAGGAGGAT GATACAGAAC AATCTATGGG AGTCAGAAAA 
AGGATAAAAG GCAAAAATAA AGGAAGGGCC AAAAAGGGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100749430 ( XM_003485330.2 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_003485378.1 Bombus impatiens probable ATP-dependent RNA helicase DDX47 (LOC100749430), mRNA.

Price & Availability↑

CloneID	OBo97284
Clone ID Related Accession (Same CDS sequence)	XM_003485330.3 , XM_003485330.2
Accession Version	XM_003485330.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	probable ATP-dependent RNA helicase DDX47
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176470.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2015 this sequence version replaced XM_003485330.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA GTAATCCAAC AAAACAACAG GAACAAGAAA ATGTAAAGGA ACTGACATGG 
AAAGATTTGG GGATCGTAGA CACATTGTGT AAAACATGTG AAGATTTAAA ATGGAAATCA 
CCTACAAAAA TTCAATGTGA AGCTATTCCG TTAACCCTTG AAGGTAAAGA TGTAATAGGA 
TTAGCAGAAA CTGGTTCTGG TAAAACTGCA GCCTTTGCTA TTCCTATATT ACAGGCACTG 
TTAGAAAATC CACAAAGATA TTTTGCTTTA ATTTTAACAC CTACCAGAGA ACTAGCCTTC 
CAAATATCAG AACAATTTGA AGCTCTAGGG TCAAGTATTG GAGTAAAATG TGCAGTAATA 
GTAGGTGGAA TGGACATGAT GTCTCAGGCA TTGTTATTGG CAAAAAAGCC ACATATTTTA 
ATTGCCACTC CAGGTAGATT AGTTGATCAC TTGGAAAACA CAAAAGGTTT TAATCTACGT 
TCTTTAAAAT TCTTGGTTAT GGATGAGGCA GATCGAATTT TGAATATGGA TTTTGAAGTT 
GAAGTAGATA AAATTTTAAG GGTAATTCCT CGAGAAAGAA GAACATTGTT GTTTTCTGCA 
ACAATGACTA AAAAGGTTCA GAAATTACAG AGAGCATCTT TGCGAAACCC TGTTAAAGTG 
GAAGTTTCAA CAAAGTACCA AACTGTTGAA AAATTACAAC AATATTATAT TTTTATCCCA 
GTTAAATTCA AGGATGTATA TCTCGTACAT ATCTTAAATG AATTAGCAGG TAACAGTTTT 
ATGATATTTT GTGCGACTTG CAACAATACA GTTAGAACTG CTCTTCTTTT ACGAAATTTG 
GGCTTTACTG CTGTTCCTTT GCATGGACAG ATGTCACAGA ATAAGAGAAT AGCTGCACTT 
ACTAAATTCA AGGCGAAAAA TCGATCTATA CTTATTTCCA CAGATGTTGC TAGCAGGGGT 
CTCGATATTC CTCATGTAGA TATTGTAATA AATTTCGATA TACCTACACA TAGTAAAGAT 
TATATACATA GAGTTGGACG TACTGCACGT GCTGGTCGTT CTGGTCGTTC CATTACGTTT 
GTAACACAGT ATGACGTAGA ATTGTATCAA AGAATAGAGC AATTGATATC AAAACAATTA 
CCATTATATC CGACTGAAGA AGAAGAAGTA ATGGTGCTCC AAGAAAGAGT AGCTGAAGCA 
CAACGTATTG TAAAAATGGA AATGAAAGAT ATCGAGGACA GTAAAAAATT AGGTAAACGA 
AAGAAGCGTC AAGATGACAA TGAGGAGGAT GATACAGAAC AATCTATGGG AGTCAGAAAA 
AGGATAAAAG GCAAAAATAA AGGAAGGGCC AAAAAGGGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003485378.1
CDS	162..1523
Translation	MEESNPTKQQEQENVKELTWKDLGIVDTLCKTCEDLKWKSPTKIQCEAIPLTLEGKDVIGLAETGSGKTAAFAIPILQALLENPQRYFALILTPTRELAFQISEQFEALGSSIGVKCAVIVGGMDMMSQALLLAKKPHILIATPGRLVDHLENTKGFNLRSLKFLVMDEADRILNMDFEVEVDKILRVIPRERRTLLFSATMTKKVQKLQRASLRNPVKVEVSTKYQTVEKLQQYYIFIPVKFKDVYLVHILNELAGNSFMIFCATCNNTVRTALLLRNLGFTAVPLHGQMSQNKRIAALTKFKAKNRSILISTDVASRGLDIPHVDIVINFDIPTHSKDYIHRVGRTARAGRSGRSITFVTQYDVELYQRIEQLISKQLPLYPTEEEEVMVLQERVAEAQRIVKMEMKDIEDSKKLGKRKKRQDDNEEDDTEQSMGVRKRIKGKNKGRAKKG

Target ORF information:

RefSeq Version	XM_003485330.2
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens probable ATP-dependent RNA helicase DDX47 (LOC100749430), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003485330.2

ORF Insert Sequence:

ATGGAAGAAA GTAATCCAAC AAAACAACAG GAACAAGAAA ATGTAAAGGA ACTGACATGG 
AAAGATTTGG GGATCGTAGA CACATTGTGT AAAACATGTG AAGATTTAAA ATGGAAATCA 
CCTACAAAAA TTCAATGTGA AGCTATTCCG TTAACCCTTG AAGGTAAAGA TGTAATAGGA 
TTAGCAGAAA CTGGTTCTGG TAAAACTGCA GCCTTTGCTA TTCCTATATT ACAGGCACTG 
TTAGAAAATC CACAAAGATA TTTTGCTTTA ATTTTAACAC CTACCAGAGA ACTAGCCTTC 
CAAATATCAG AACAATTTGA AGCTCTAGGG TCAAGTATTG GAGTAAAATG TGCAGTAATA 
GTAGGTGGAA TGGACATGAT GTCTCAGGCA TTGTTATTGG CAAAAAAGCC ACATATTTTA 
ATTGCCACTC CAGGTAGATT AGTTGATCAC TTGGAAAACA CAAAAGGTTT TAATCTACGT 
TCTTTAAAAT TCTTGGTTAT GGATGAGGCA GATCGAATTT TGAATATGGA TTTTGAAGTT 
GAAGTAGATA AAATTTTAAG GGTAATTCCT CGAGAAAGAA GAACATTGTT GTTTTCTGCA 
ACAATGACTA AAAAGGTTCA GAAATTACAG AGAGCATCTT TGCGAAACCC TGTTAAAGTG 
GAAGTTTCAA CAAAGTACCA AACTGTTGAA AAATTACAAC AATATTATAT TTTTATCCCA 
GTTAAATTCA AGGATGTATA TCTCGTACAT ATCTTAAATG AATTAGCAGG TAACAGTTTT 
ATGATATTTT GTGCGACTTG CAACAATACA GTTAGAACTG CTCTTCTTTT ACGAAATTTG 
GGCTTTACTG CTGTTCCTTT GCATGGACAG ATGTCACAGA ATAAGAGAAT AGCTGCACTT 
ACTAAATTCA AGGCGAAAAA TCGATCTATA CTTATTTCCA CAGATGTTGC TAGCAGGGGT 
CTCGATATTC CTCATGTAGA TATTGTAATA AATTTCGATA TACCTACACA TAGTAAAGAT 
TATATACATA GAGTTGGACG TACTGCACGT GCTGGTCGTT CTGGTCGTTC CATTACGTTT 
GTAACACAGT ATGACGTAGA ATTGTATCAA AGAATAGAGC AATTGATATC AAAACAATTA 
CCATTATATC CGACTGAAGA AGAAGAAGTA ATGGTGCTCC AAGAAAGAGT AGCTGAAGCA 
CAACGTATTG TAAAAATGGA AATGAAAGAT ATCGAGGACA GTAAAAAATT AGGTAAACGA 
AAGAAGCGTC AAGATGACAA TGAGGAGGAT GATACAGAAC AATCTATGGG AGTCAGAAAA 
AGGATAAAAG GCAAAAATAA AGGAAGGGCC AAAAAGGGTT AA