LOC100749006 cDNA ORF clone, Bombus impatiens(common eastern bumble bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100749006
Entrez Gene ID	100749006
Full Name	uncharacterized LOC100749006
Gene Type	protein-coding
Organism	Bombus impatiens(common eastern bumble bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012387845.2	XP_012243268.1	uncharacterized protein LOC100749006 isoform X3
XM_024368866.1	XP_024224634.1	uncharacterized protein LOC100749006 isoform X3
XM_012387847.1	XP_012243270.1	uncharacterized protein LOC100749006 isoform X3
XM_003490922.3	XP_003490970.1	uncharacterized protein LOC100749006 isoform X1
XM_024368865.1	XP_024224633.1	uncharacterized protein LOC100749006 isoform X2
XM_012387845.1	XP_012243268.1	uncharacterized protein LOC100749006 isoform X2
XM_012387846.1	XP_012243269.1	uncharacterized protein LOC100749006 isoform X2
XM_003490922.2	XP_003490970.1	uncharacterized protein LOC100749006 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100749006 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100749006 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBo106113	XM_024368866.1 Latest version!	Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBo106113	XM_012387846.1 Latest version!	Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBo106113	XM_012387845.2 Latest version!	Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBo106114	XM_012387847.1 Latest version!	Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBo106112	XM_003490922.3 Latest version!	Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBo115590	XM_024368865.1 Latest version!	Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OBo106113	XM_012387845.1	Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBo106112	XM_003490922.2	Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100749006 ( XM_012387845.2 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012243268.1 Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X3, mRNA.

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CloneID	OBo106113
Clone ID Related Accession (Same CDS sequence)	XM_012387845.2 , XM_024368866.1 , XM_012387845.1 , XM_012387846.1
Accession Version	XM_012387845.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	uncharacterized protein LOC100749006 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176906.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 19, 2018 this sequence version replaced XM_012387845.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGAT TTTTCAACTC CCTGAAAAAC TTCGCGTCAG GAGCCGCGTA CGCTGCCAGC 
TCTGCCTCGA AGTACGAAGA ATTGGAAGTC GAAGACACGA AGATGAGATT TAGCCTGACG 
CCGCAACCAG GAGAAAGTGG TTTTATACAA TGGCTGGACG CGATGAAAAT GGTTGCCAGG 
TTACCAGGAG GAATTCCGCC GGAATTTCGA AAGAAGTTGT GGCTGACACT GGCAGAACGT 
CATCTAGAGC AGCGCGGCGT AGATTGGAAG CAGGCTGAAA AGATCTGCTT CAACGAGTGG 
AGCAATCCTG ACGATGAAGA GCTTGGTATA CAAATCGTTA AAGACCTACA CAGAACGGGC 
TGCAGTCTGT TTTGCGGTGC AGCTGGCAGA GACAATCAAG CAGTGCTTCG TCGAGTTCTG 
CTTGGTTTCG CCCGATGGAA CAAGTCCGTC GGCTATTGCC AGGGCCTGAA CGTGTTGGCC 
GCCCTCGTTT TGCAAGTAAT GGATCGCGCC GAGTCCGCCG CGGTGAAGGT GATGATCTAC 
TTGATAGAGG GTGTCCTGCC CGAGGGTTAC TTCGCGGACA ATCTCCGTGG CCTGTCGGTC 
GACATGGCGG TATTTCGAGA TCTCCTCCGG GGCAGACTGC CCAAGCTCTC GAAACATCTG 
GAGGCTCTGC AGAACGATGC TAAGGACAAG GCGACAGGCA GCAGTTACGA GCCACCTCTG 
ACGAATGTGT TCACGATGCA ATGGTTCCTT ACTCTTTTCT GTCACTGTTT GCCACAAGAG 
GCTGTCCTGC GCGTATGGGA CCTCATATTT CTCGAAGGAG ATGAGATATT GCTTAGAACC 
GCACTGGCCA TCTGGGAAGG ACTTTCCGAT CGTATTATGA CGGTGACATC GGCGGACGAG 
TTTTATAGTA TAATGGGAGT GCTGACAAGG GAGATGCTCG AGTTCACGGA TACTAATAAC 
CTCATAAAGA ATATCGTTAG TATGGGATCT TTGCATGGTG TAACGGGGCT AAGAGAGAAA 
CATAGATACA ATATCACACC TTGGGCAAGA AAATTGAGCG ACGACGAGGA CAGCGACACG 
GAGGAGGATG AAAGGCTGGC CGTGGCTGCT GCGATGTTCA GTATGGCACA GCGATTGAAG 
AAAGATCGTA TACCTCAAAC GATTGGAGCG CTGCAGGCAA TGGCACCCAG CAGCGACCGA 
GAACGGCTCG CTTTGGACAT TACCACTTTG AAACAGCAGT ACGCCAAGCT ACGCGAACGT 
CAACGACAAG CGCATATTAT ACTTTCTGCT GCATGTGCAA GGCAGACCAT GGTACCACCT 
CCTACTTCTC AGGCCATGAA TCATCTTTTA GTGGGCAAAA GTGCGCTCGT GAGTGGGAAG 
AATCGACCCC TGAGCCTACC TTCTGGTGCT GCAACGACGA AGATAAGACC TACGACGCTA 
AATCAAAACC GAAGGGACAG ACAAGGCGTC ACGCTTCACT GGAAAGACAC CAAAAAACCG 
AAGCAGAAAC CCTCGAATTT ACCAGAAACG GTGGAATGTA GCGTTGTAAC AATGCAATCC 
ACGGAAGCTG AGCTGGCTTC TCCAAAGCGG AGTAACGGTG ACAGTGATAG CGACAGCACA 
TCGACGGAAT TGTGCGACGA ACCGGACCGT CTCAGTGACG TTGACAGCGA AGATCTGACG 
TCAGCATCCG AATCCTGTGT TGTCAGTACG GATGAGGAGA AAAGCTCTCG AATCACCGGA 
TCACCGATTC GCGAGAAGCC ACTCGATCGT TCGTTTCTCG AAGAGAAGAT GGAACCAATT 
AACGCCGAAG ATTCGAAGGA CTCGAAAAGC TCCGAGGATA ACCTTGGTGA CATATCAATC 
GCTAAAATCA CCGACCAAAT AAGACGATTA TCAGCAGAGG ATGACAATAA TTCCATGGTT 
CCTCAAAAAT TTAGCGTGCA GAGCAAAGAA TCGCCGGAAG AACAATCGAC GAAAGAATCG 
AAAAAAGAGA AATCCATATC AGATCTATCT ATAGATTACA CTTCCGATAG TAATATCATT 
AAAAAATCGT TATTGGACTT GAACGAGTAC GATTATCTAG GTTTCAGTGG CAGTAAGATC 
AGTTCGAAAG AAGATGAAGT ATTAGTGCAC GAAAGGCATC GATCTAAACA GACAACAAAC 
GAGATTGTAG ATATACCTCG AAACGAAGTT GCGACAATCA AGAAAGACCA AACTGAAGAC 
AAGATAACTA CGACTATGAA ACAGGATTCT TTAAACATAT ACTTGGATTA TTCAGCATCC 
TTGGACAAGA AATACGATAG AATCAAAGAT AAATCAAGCC TCGATGTAGA GATAGAAAAT 
GAAATTAGAT CCGACCGATA TAAAACATCC TTCGATTTCT CTAGTAGAAT ATCTTTGGAC 
TTGAAACCGT ATGAAACTTC AAAAGTAGGA AGTTTAACCA GTGACACTGC AGACACAGTG 
AATACTGAAA GAACGCTTTA TGGCAAGGCC TACGTTGGTC ATCTTCCTAT TTCACCTGTA 
ACAGTTGATC AAAAATTAAG CAGAGTGACA CCCAGTATAC ATACAACGCA TAATACTACT 
GTATTAACTC CTGAAACGCC TGAAATTGTA CATAAAACGT TAGAGAACAC GTCATATAAC 
GATAGAATAC AGACGACTCA AACGAAAATT TATTCAGACC TAGTGAAGAG TCCTAAAACA 
CCGGAAAGCA ACAAATCATT CAGTTCAGGT GAAACCATGG GACCAGATTC GAAACCATCG 
AGTCTGACAG TTAGCCCAAG GACGCCGGTT AGATCAGAAT CTCGAGATTT CCCTATGGAC 
AAATCCCTGT GTTCGTCAGT TAGCTCAGAC TCGAAGACTT TGGTTCTTCA TTCTGCAGAT 
TCAGACAGTA CGAGGGACAG CACGAAAACC GACGCGAAGA AAATGTATGA GATTTCCAAT 
TCGGCGACAC CTATCAGCAT GGACTCGTTG CAGAAATCAG AAGCCAGCCC AAAACTAGTC 
GTGAGCAGCT CCAGTGAATC GTGTAGCCCG AGTAAATTAA AGTCTTCCGA GAGGTCGTTC 
AGTTCGGACC TGCAATCGCC AATAAGTGCC AACTCCATAA AGTATACTTC GAATTTCAGT 
AGACGGGGCC AAGATGACGT ATCAGGCTCG CCAATGGACT TGAGCAGCGC TACCTCGCCG 
TTTTATCCGA TCTCGAATCC GAATCAAAAA ACTCCGACGT CCCCGTACAG TGCTTCTAGA 
AGAAGATCCA GTTTGGACAG CAACGAAACG TCACCTGAGC AGAAATCGAC CAGTTCGAGG 
GAGTCGAACA AGTTTCTAGG TTATCAGTCG AAATTTGACT CCTCCATAAA GACGTCTGAT 
ATGAAAGATG TAGACGGTAT AGCACGAAAA GGTGACACAT TCGTCAGACC AGAGTTCATC 
ACGAGGAATG AAGATTCGAG TATTAATTTG ACGGATAGAA GAATCAATGT TAGTGATGTT 
AAGTATTATC AAACAACGAG TACGGATTAC TATCGCAAGG ATAAGGATAT TTTTGATGAC 
GGAGGGGATG ATCCGTCATC GGAGAAAGAC GTGGTACCTT CTTTGCCTCA GGAGAAGCTA 
GATAAACATT ATTTGAACTA TCGATATAGA GATAGATCGA AGTTGTTGGA AAGAAGTTCA 
AGCAGTTTGG ACAGTAGAAG GTACGCTGAT ATGAAGTCAT CGGCTTCCAC CGATGAATTT 
TCAACGTCGA TCGCGCAGAA GAGATCCAGT GACATTTTAG AAGATATTAA ACACTTGGAG 
GCGAAAGCAA ACGATGGGTC GTCGGATAAT GGGATGTTGA CGAAGAAGAG CAGTTATATT 
TGGGAGGATA TGAAGAATTT AGAAGCCAGG AGGCAAGATA CCTTTAGTGA TTCAGCGAGG 
AACTTTTCGA AACATCGTCT GGAGGTACCG GATATAAATA TAACCGGAGA AAGCAGAAAA 
TCGTACGTAG TACATCCGAA GATCAATATC GACGAAAATA GCGACAGCAT ATTGAAGACC 
ATGGCCTGTG GTAAGAGTAA CGGCACAGAT GATGGCAGGG AGTCGAAGTT GGGCGTGTGG 
ACGAAGGTGA AGCCTCGTAA AAAGAGTGAC AATGGGCGGA GGAACAGCGA CAGGGCGTTG 
AAGATCATTC AAGAAAACTC CGCTATACTT CAGAAGATTC TTGCGTGTCA GGCAAAAAAG 
CGTCTGCCAG ACCTGGAGGA GATATCGAAA GAAATTACCA TTAGCCCTAT AAACGAGGAG 
ATTTCGAAGA TCTTTAGTCC GATATTAGAG AAAATGGGAT TAAACGAACA CGAGATTAAC 
GAGGAATTGG CGAGAATCAA TTTCAAGGAT TTCGACAACA TGACCGCTAC CAGTGTGTCC 
GAGTTTGATG CAAAGATTAA CGAGGAATTA TCGAAGCTTT CTCTGATCGA CGAGACGGAG 
CAGATGGACC ACTTCGACGT GGACGAGGTG ATATCCCACG AATACTTGAA CACGAGAGAA 
GCTCTAATAG ATCGTAAGAT AAACGAGGAA TTGTCGAAAT TATTGGCAAA CTACGACGAT 
CATTCTCCGG CTAGTATGGT GAGTTTGGAC AAAGGACCAT CCAGCCAGAA TATTAGCGAG 
ATCGAAGGAC TGGATCTGTC CAGTATTTCA ACTAACGTAT TTTCCTATAA ATCATCCAAT 
GACTCCATTG ACACGAGAAG CGACTTAGGC TCGACGCAGG AACCATCCAT TCTCGTTGAC 
AAGTTTCCGA AATATACGGC ATACGCCGTA CCGAAGTATC ACGTGGACGA CTCATCACCT 
AAAAGCGACA TAGACATCTA CAGAGAATTG GAGAAACTCG ATAAGATCTC TTCCGCACAG 
GTGTTACCAG ATCCGACTCT GGAGCTTCCA CAGAAGGAAT TGTCCTCGAT TCCGTACGTT 
CCAAATACGT CGCCCTTCAA GGATGTGTCA CCATTGAGGT ACACTTCCAA GCCGGCTCAA 
TACGACACTT CACCCCTGAA GACTTCTTAC GATCCCTACA AGACCTTTGA CTTCAAGCCC 
AAGTTGTCAC CTAAACAAGT TTCCACTAAT CCATTTGTGT CAGCTACGTA TGAGAAACCT 
GTGGTGGATA CCGCTTTCGA GTACATTAAC AATAAACCAG AGATCTCGAT CAATACGTAT 
GATCTACATT TGAAGAGTCC AATGATGACG AAGGAATCGC TAGAGTTTCG TGTCAGATAC 
GACGATGATC CAGTCAGAGA AGTTAACACC GATTACATGT CTCTCCAGCC AGAAAGTAGA 
TCGATTACTA CCCCGAGCAA GTCTCCTTAT TTCAGCAATG GAAATACCGA ACCGTTAACG 
CCTACGAAAT ATATTTCTAA GGAGGAAAGA ATCTTGGATA CTGGTGGTAC CTCGTTTCTG 
AACTATCCCA GCGAATCTTC CGACCTTCTT CGTCGAAAAT ACGACGCTTT GTCTCCTAAG 
GAGTACACTC ATACCAAGAG TACGACGGAT TATGATAGAC ACTTAGGCAC GTTACCTCCT 
ATAGAACCTG GAACAGAGAC AGGATCGTAT TTACCGACGA GACACCGAGA TTATCATTCT 
CTATCACCGA ATCGTCAGTT TCCTGATCAC TTTCCTTCTA CTATCAATCA TCACTACACC 
TCGAAACTTT CATCAGGTCT CTCTGACGAA TCGAAACGAC CGGTTTCTCA TCCGGAATTC 
CCTGGCAAGA TGAATGTTGG GAAATATGCA GAATTACCCG ATAGAAGTTC CAGCAGTTAC 
AAGAAATCAT CTCTTAGCTT AGGTAACATA GGACACTCGA GTAAAGGAGC TGATAATAAT 
TTCAACACTG TGACCAGTCC AAAAACGCAG TTCAGTCCCT TTCCCGTTAG AAACGCTCCT 
AGGAAGCCGA AAGAACTTAC GCTGAAATTG GGCCTCTATT CGCCCAAAGG TTCTGATACA 
GGACAGTTAA AAAGGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012243268.1
CDS	416..6376
Translation	MSGFFNSLKNFASGAAYAASSASKYEELEVEDTKMRFSLTPQPGESGFIQWLDAMKMVARLPGGIPPEFRKKLWLTLAERHLEQRGVDWKQAEKICFNEWSNPDDEELGIQIVKDLHRTGCSLFCGAAGRDNQAVLRRVLLGFARWNKSVGYCQGLNVLAALVLQVMDRAESAAVKVMIYLIEGVLPEGYFADNLRGLSVDMAVFRDLLRGRLPKLSKHLEALQNDAKDKATGSSYEPPLTNVFTMQWFLTLFCHCLPQEAVLRVWDLIFLEGDEILLRTALAIWEGLSDRIMTVTSADEFYSIMGVLTREMLEFTDTNNLIKNIVSMGSLHGVTGLREKHRYNITPWARKLSDDEDSDTEEDERLAVAAAMFSMAQRLKKDRIPQTIGALQAMAPSSDRERLALDITTLKQQYAKLRERQRQAHIILSAACARQTMVPPPTSQAMNHLLVGKSALVSGKNRPLSLPSGAATTKIRPTTLNQNRRDRQGVTLHWKDTKKPKQKPSNLPETVECSVVTMQSTEAELASPKRSNGDSDSDSTSTELCDEPDRLSDVDSEDLTSASESCVVSTDEEKSSRITGSPIREKPLDRSFLEEKMEPINAEDSKDSKSSEDNLGDISIAKITDQIRRLSAEDDNNSMVPQKFSVQSKESPEEQSTKESKKEKSISDLSIDYTSDSNIIKKSLLDLNEYDYLGFSGSKISSKEDEVLVHERHRSKQTTNEIVDIPRNEVATIKKDQTEDKITTTMKQDSLNIYLDYSASLDKKYDRIKDKSSLDVEIENEIRSDRYKTSFDFSSRISLDLKPYETSKVGSLTSDTADTVNTERTLYGKAYVGHLPISPVTVDQKLSRVTPSIHTTHNTTVLTPETPEIVHKTLENTSYNDRIQTTQTKIYSDLVKSPKTPESNKSFSSGETMGPDSKPSSLTVSPRTPVRSESRDFPMDKSLCSSVSSDSKTLVLHSADSDSTRDSTKTDAKKMYEISNSATPISMDSLQKSEASPKLVVSSSSESCSPSKLKSSERSFSSDLQSPISANSIKYTSNFSRRGQDDVSGSPMDLSSATSPFYPISNPNQKTPTSPYSASRRRSSLDSNETSPEQKSTSSRESNKFLGYQSKFDSSIKTSDMKDVDGIARKGDTFVRPEFITRNEDSSINLTDRRINVSDVKYYQTTSTDYYRKDKDIFDDGGDDPSSEKDVVPSLPQEKLDKHYLNYRYRDRSKLLERSSSSLDSRRYADMKSSASTDEFSTSIAQKRSSDILEDIKHLEAKANDGSSDNGMLTKKSSYIWEDMKNLEARRQDTFSDSARNFSKHRLEVPDINITGESRKSYVVHPKINIDENSDSILKTMACGKSNGTDDGRESKLGVWTKVKPRKKSDNGRRNSDRALKIIQENSAILQKILACQAKKRLPDLEEISKEITISPINEEISKIFSPILEKMGLNEHEINEELARINFKDFDNMTATSVSEFDAKINEELSKLSLIDETEQMDHFDVDEVISHEYLNTREALIDRKINEELSKLLANYDDHSPASMVSLDKGPSSQNISEIEGLDLSSISTNVFSYKSSNDSIDTRSDLGSTQEPSILVDKFPKYTAYAVPKYHVDDSSPKSDIDIYRELEKLDKISSAQVLPDPTLELPQKELSSIPYVPNTSPFKDVSPLRYTSKPAQYDTSPLKTSYDPYKTFDFKPKLSPKQVSTNPFVSATYEKPVVDTAFEYINNKPEISINTYDLHLKSPMMTKESLEFRVRYDDDPVREVNTDYMSLQPESRSITTPSKSPYFSNGNTEPLTPTKYISKEERILDTGGTSFLNYPSESSDLLRRKYDALSPKEYTHTKSTTDYDRHLGTLPPIEPGTETGSYLPTRHRDYHSLSPNRQFPDHFPSTINHHYTSKLSSGLSDESKRPVSHPEFPGKMNVGKYAELPDRSSSSYKKSSLSLGNIGHSSKGADNNFNTVTSPKTQFSPFPVRNAPRKPKELTLKLGLYSPKGSDTGQLKRS

Target ORF information:

RefSeq Version	XM_012387845.2
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012387845.2

ORF Insert Sequence:

ATGAGCGGAT TTTTCAACTC CCTGAAAAAC TTCGCGTCAG GAGCCGCGTA CGCTGCCAGC 
TCTGCCTCGA AGTACGAAGA ATTGGAAGTC GAAGACACGA AGATGAGATT TAGCCTGACG 
CCGCAACCAG GAGAAAGTGG TTTTATACAA TGGCTGGACG CGATGAAAAT GGTTGCCAGG 
TTACCAGGAG GAATTCCGCC GGAATTTCGA AAGAAGTTGT GGCTGACACT GGCAGAACGT 
CATCTAGAGC AGCGCGGCGT AGATTGGAAG CAGGCTGAAA AGATCTGCTT CAACGAGTGG 
AGCAATCCTG ACGATGAAGA GCTTGGTATA CAAATCGTTA AAGACCTACA CAGAACGGGC 
TGCAGTCTGT TTTGCGGTGC AGCTGGCAGA GACAATCAAG CAGTGCTTCG TCGAGTTCTG 
CTTGGTTTCG CCCGATGGAA CAAGTCCGTC GGCTATTGCC AGGGCCTGAA CGTGTTGGCC 
GCCCTCGTTT TGCAAGTAAT GGATCGCGCC GAGTCCGCCG CGGTGAAGGT GATGATCTAC 
TTGATAGAGG GTGTCCTGCC CGAGGGTTAC TTCGCGGACA ATCTCCGTGG CCTGTCGGTC 
GACATGGCGG TATTTCGAGA TCTCCTCCGG GGCAGACTGC CCAAGCTCTC GAAACATCTG 
GAGGCTCTGC AGAACGATGC TAAGGACAAG GCGACAGGCA GCAGTTACGA GCCACCTCTG 
ACGAATGTGT TCACGATGCA ATGGTTCCTT ACTCTTTTCT GTCACTGTTT GCCACAAGAG 
GCTGTCCTGC GCGTATGGGA CCTCATATTT CTCGAAGGAG ATGAGATATT GCTTAGAACC 
GCACTGGCCA TCTGGGAAGG ACTTTCCGAT CGTATTATGA CGGTGACATC GGCGGACGAG 
TTTTATAGTA TAATGGGAGT GCTGACAAGG GAGATGCTCG AGTTCACGGA TACTAATAAC 
CTCATAAAGA ATATCGTTAG TATGGGATCT TTGCATGGTG TAACGGGGCT AAGAGAGAAA 
CATAGATACA ATATCACACC TTGGGCAAGA AAATTGAGCG ACGACGAGGA CAGCGACACG 
GAGGAGGATG AAAGGCTGGC CGTGGCTGCT GCGATGTTCA GTATGGCACA GCGATTGAAG 
AAAGATCGTA TACCTCAAAC GATTGGAGCG CTGCAGGCAA TGGCACCCAG CAGCGACCGA 
GAACGGCTCG CTTTGGACAT TACCACTTTG AAACAGCAGT ACGCCAAGCT ACGCGAACGT 
CAACGACAAG CGCATATTAT ACTTTCTGCT GCATGTGCAA GGCAGACCAT GGTACCACCT 
CCTACTTCTC AGGCCATGAA TCATCTTTTA GTGGGCAAAA GTGCGCTCGT GAGTGGGAAG 
AATCGACCCC TGAGCCTACC TTCTGGTGCT GCAACGACGA AGATAAGACC TACGACGCTA 
AATCAAAACC GAAGGGACAG ACAAGGCGTC ACGCTTCACT GGAAAGACAC CAAAAAACCG 
AAGCAGAAAC CCTCGAATTT ACCAGAAACG GTGGAATGTA GCGTTGTAAC AATGCAATCC 
ACGGAAGCTG AGCTGGCTTC TCCAAAGCGG AGTAACGGTG ACAGTGATAG CGACAGCACA 
TCGACGGAAT TGTGCGACGA ACCGGACCGT CTCAGTGACG TTGACAGCGA AGATCTGACG 
TCAGCATCCG AATCCTGTGT TGTCAGTACG GATGAGGAGA AAAGCTCTCG AATCACCGGA 
TCACCGATTC GCGAGAAGCC ACTCGATCGT TCGTTTCTCG AAGAGAAGAT GGAACCAATT 
AACGCCGAAG ATTCGAAGGA CTCGAAAAGC TCCGAGGATA ACCTTGGTGA CATATCAATC 
GCTAAAATCA CCGACCAAAT AAGACGATTA TCAGCAGAGG ATGACAATAA TTCCATGGTT 
CCTCAAAAAT TTAGCGTGCA GAGCAAAGAA TCGCCGGAAG AACAATCGAC GAAAGAATCG 
AAAAAAGAGA AATCCATATC AGATCTATCT ATAGATTACA CTTCCGATAG TAATATCATT 
AAAAAATCGT TATTGGACTT GAACGAGTAC GATTATCTAG GTTTCAGTGG CAGTAAGATC 
AGTTCGAAAG AAGATGAAGT ATTAGTGCAC GAAAGGCATC GATCTAAACA GACAACAAAC 
GAGATTGTAG ATATACCTCG AAACGAAGTT GCGACAATCA AGAAAGACCA AACTGAAGAC 
AAGATAACTA CGACTATGAA ACAGGATTCT TTAAACATAT ACTTGGATTA TTCAGCATCC 
TTGGACAAGA AATACGATAG AATCAAAGAT AAATCAAGCC TCGATGTAGA GATAGAAAAT 
GAAATTAGAT CCGACCGATA TAAAACATCC TTCGATTTCT CTAGTAGAAT ATCTTTGGAC 
TTGAAACCGT ATGAAACTTC AAAAGTAGGA AGTTTAACCA GTGACACTGC AGACACAGTG 
AATACTGAAA GAACGCTTTA TGGCAAGGCC TACGTTGGTC ATCTTCCTAT TTCACCTGTA 
ACAGTTGATC AAAAATTAAG CAGAGTGACA CCCAGTATAC ATACAACGCA TAATACTACT 
GTATTAACTC CTGAAACGCC TGAAATTGTA CATAAAACGT TAGAGAACAC GTCATATAAC 
GATAGAATAC AGACGACTCA AACGAAAATT TATTCAGACC TAGTGAAGAG TCCTAAAACA 
CCGGAAAGCA ACAAATCATT CAGTTCAGGT GAAACCATGG GACCAGATTC GAAACCATCG 
AGTCTGACAG TTAGCCCAAG GACGCCGGTT AGATCAGAAT CTCGAGATTT CCCTATGGAC 
AAATCCCTGT GTTCGTCAGT TAGCTCAGAC TCGAAGACTT TGGTTCTTCA TTCTGCAGAT 
TCAGACAGTA CGAGGGACAG CACGAAAACC GACGCGAAGA AAATGTATGA GATTTCCAAT 
TCGGCGACAC CTATCAGCAT GGACTCGTTG CAGAAATCAG AAGCCAGCCC AAAACTAGTC 
GTGAGCAGCT CCAGTGAATC GTGTAGCCCG AGTAAATTAA AGTCTTCCGA GAGGTCGTTC 
AGTTCGGACC TGCAATCGCC AATAAGTGCC AACTCCATAA AGTATACTTC GAATTTCAGT 
AGACGGGGCC AAGATGACGT ATCAGGCTCG CCAATGGACT TGAGCAGCGC TACCTCGCCG 
TTTTATCCGA TCTCGAATCC GAATCAAAAA ACTCCGACGT CCCCGTACAG TGCTTCTAGA 
AGAAGATCCA GTTTGGACAG CAACGAAACG TCACCTGAGC AGAAATCGAC CAGTTCGAGG 
GAGTCGAACA AGTTTCTAGG TTATCAGTCG AAATTTGACT CCTCCATAAA GACGTCTGAT 
ATGAAAGATG TAGACGGTAT AGCACGAAAA GGTGACACAT TCGTCAGACC AGAGTTCATC 
ACGAGGAATG AAGATTCGAG TATTAATTTG ACGGATAGAA GAATCAATGT TAGTGATGTT 
AAGTATTATC AAACAACGAG TACGGATTAC TATCGCAAGG ATAAGGATAT TTTTGATGAC 
GGAGGGGATG ATCCGTCATC GGAGAAAGAC GTGGTACCTT CTTTGCCTCA GGAGAAGCTA 
GATAAACATT ATTTGAACTA TCGATATAGA GATAGATCGA AGTTGTTGGA AAGAAGTTCA 
AGCAGTTTGG ACAGTAGAAG GTACGCTGAT ATGAAGTCAT CGGCTTCCAC CGATGAATTT 
TCAACGTCGA TCGCGCAGAA GAGATCCAGT GACATTTTAG AAGATATTAA ACACTTGGAG 
GCGAAAGCAA ACGATGGGTC GTCGGATAAT GGGATGTTGA CGAAGAAGAG CAGTTATATT 
TGGGAGGATA TGAAGAATTT AGAAGCCAGG AGGCAAGATA CCTTTAGTGA TTCAGCGAGG 
AACTTTTCGA AACATCGTCT GGAGGTACCG GATATAAATA TAACCGGAGA AAGCAGAAAA 
TCGTACGTAG TACATCCGAA GATCAATATC GACGAAAATA GCGACAGCAT ATTGAAGACC 
ATGGCCTGTG GTAAGAGTAA CGGCACAGAT GATGGCAGGG AGTCGAAGTT GGGCGTGTGG 
ACGAAGGTGA AGCCTCGTAA AAAGAGTGAC AATGGGCGGA GGAACAGCGA CAGGGCGTTG 
AAGATCATTC AAGAAAACTC CGCTATACTT CAGAAGATTC TTGCGTGTCA GGCAAAAAAG 
CGTCTGCCAG ACCTGGAGGA GATATCGAAA GAAATTACCA TTAGCCCTAT AAACGAGGAG 
ATTTCGAAGA TCTTTAGTCC GATATTAGAG AAAATGGGAT TAAACGAACA CGAGATTAAC 
GAGGAATTGG CGAGAATCAA TTTCAAGGAT TTCGACAACA TGACCGCTAC CAGTGTGTCC 
GAGTTTGATG CAAAGATTAA CGAGGAATTA TCGAAGCTTT CTCTGATCGA CGAGACGGAG 
CAGATGGACC ACTTCGACGT GGACGAGGTG ATATCCCACG AATACTTGAA CACGAGAGAA 
GCTCTAATAG ATCGTAAGAT AAACGAGGAA TTGTCGAAAT TATTGGCAAA CTACGACGAT 
CATTCTCCGG CTAGTATGGT GAGTTTGGAC AAAGGACCAT CCAGCCAGAA TATTAGCGAG 
ATCGAAGGAC TGGATCTGTC CAGTATTTCA ACTAACGTAT TTTCCTATAA ATCATCCAAT 
GACTCCATTG ACACGAGAAG CGACTTAGGC TCGACGCAGG AACCATCCAT TCTCGTTGAC 
AAGTTTCCGA AATATACGGC ATACGCCGTA CCGAAGTATC ACGTGGACGA CTCATCACCT 
AAAAGCGACA TAGACATCTA CAGAGAATTG GAGAAACTCG ATAAGATCTC TTCCGCACAG 
GTGTTACCAG ATCCGACTCT GGAGCTTCCA CAGAAGGAAT TGTCCTCGAT TCCGTACGTT 
CCAAATACGT CGCCCTTCAA GGATGTGTCA CCATTGAGGT ACACTTCCAA GCCGGCTCAA 
TACGACACTT CACCCCTGAA GACTTCTTAC GATCCCTACA AGACCTTTGA CTTCAAGCCC 
AAGTTGTCAC CTAAACAAGT TTCCACTAAT CCATTTGTGT CAGCTACGTA TGAGAAACCT 
GTGGTGGATA CCGCTTTCGA GTACATTAAC AATAAACCAG AGATCTCGAT CAATACGTAT 
GATCTACATT TGAAGAGTCC AATGATGACG AAGGAATCGC TAGAGTTTCG TGTCAGATAC 
GACGATGATC CAGTCAGAGA AGTTAACACC GATTACATGT CTCTCCAGCC AGAAAGTAGA 
TCGATTACTA CCCCGAGCAA GTCTCCTTAT TTCAGCAATG GAAATACCGA ACCGTTAACG 
CCTACGAAAT ATATTTCTAA GGAGGAAAGA ATCTTGGATA CTGGTGGTAC CTCGTTTCTG 
AACTATCCCA GCGAATCTTC CGACCTTCTT CGTCGAAAAT ACGACGCTTT GTCTCCTAAG 
GAGTACACTC ATACCAAGAG TACGACGGAT TATGATAGAC ACTTAGGCAC GTTACCTCCT 
ATAGAACCTG GAACAGAGAC AGGATCGTAT TTACCGACGA GACACCGAGA TTATCATTCT 
CTATCACCGA ATCGTCAGTT TCCTGATCAC TTTCCTTCTA CTATCAATCA TCACTACACC 
TCGAAACTTT CATCAGGTCT CTCTGACGAA TCGAAACGAC CGGTTTCTCA TCCGGAATTC 
CCTGGCAAGA TGAATGTTGG GAAATATGCA GAATTACCCG ATAGAAGTTC CAGCAGTTAC 
AAGAAATCAT CTCTTAGCTT AGGTAACATA GGACACTCGA GTAAAGGAGC TGATAATAAT 
TTCAACACTG TGACCAGTCC AAAAACGCAG TTCAGTCCCT TTCCCGTTAG AAACGCTCCT 
AGGAAGCCGA AAGAACTTAC GCTGAAATTG GGCCTCTATT CGCCCAAAGG TTCTGATACA 
GGACAGTTAA AAAGGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100749006 ( XM_024368866.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_024224634.1 Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X4, mRNA.

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CloneID	OBo106113
Clone ID Related Accession (Same CDS sequence)	XM_012387845.2 , XM_024368866.1 , XM_012387845.1 , XM_012387846.1
Accession Version	XM_024368866.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	uncharacterized protein LOC100749006 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176906.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGAT TTTTCAACTC CCTGAAAAAC TTCGCGTCAG GAGCCGCGTA CGCTGCCAGC 
TCTGCCTCGA AGTACGAAGA ATTGGAAGTC GAAGACACGA AGATGAGATT TAGCCTGACG 
CCGCAACCAG GAGAAAGTGG TTTTATACAA TGGCTGGACG CGATGAAAAT GGTTGCCAGG 
TTACCAGGAG GAATTCCGCC GGAATTTCGA AAGAAGTTGT GGCTGACACT GGCAGAACGT 
CATCTAGAGC AGCGCGGCGT AGATTGGAAG CAGGCTGAAA AGATCTGCTT CAACGAGTGG 
AGCAATCCTG ACGATGAAGA GCTTGGTATA CAAATCGTTA AAGACCTACA CAGAACGGGC 
TGCAGTCTGT TTTGCGGTGC AGCTGGCAGA GACAATCAAG CAGTGCTTCG TCGAGTTCTG 
CTTGGTTTCG CCCGATGGAA CAAGTCCGTC GGCTATTGCC AGGGCCTGAA CGTGTTGGCC 
GCCCTCGTTT TGCAAGTAAT GGATCGCGCC GAGTCCGCCG CGGTGAAGGT GATGATCTAC 
TTGATAGAGG GTGTCCTGCC CGAGGGTTAC TTCGCGGACA ATCTCCGTGG CCTGTCGGTC 
GACATGGCGG TATTTCGAGA TCTCCTCCGG GGCAGACTGC CCAAGCTCTC GAAACATCTG 
GAGGCTCTGC AGAACGATGC TAAGGACAAG GCGACAGGCA GCAGTTACGA GCCACCTCTG 
ACGAATGTGT TCACGATGCA ATGGTTCCTT ACTCTTTTCT GTCACTGTTT GCCACAAGAG 
GCTGTCCTGC GCGTATGGGA CCTCATATTT CTCGAAGGAG ATGAGATATT GCTTAGAACC 
GCACTGGCCA TCTGGGAAGG ACTTTCCGAT CGTATTATGA CGGTGACATC GGCGGACGAG 
TTTTATAGTA TAATGGGAGT GCTGACAAGG GAGATGCTCG AGTTCACGGA TACTAATAAC 
CTCATAAAGA ATATCGTTAG TATGGGATCT TTGCATGGTG TAACGGGGCT AAGAGAGAAA 
CATAGATACA ATATCACACC TTGGGCAAGA AAATTGAGCG ACGACGAGGA CAGCGACACG 
GAGGAGGATG AAAGGCTGGC CGTGGCTGCT GCGATGTTCA GTATGGCACA GCGATTGAAG 
AAAGATCGTA TACCTCAAAC GATTGGAGCG CTGCAGGCAA TGGCACCCAG CAGCGACCGA 
GAACGGCTCG CTTTGGACAT TACCACTTTG AAACAGCAGT ACGCCAAGCT ACGCGAACGT 
CAACGACAAG CGCATATTAT ACTTTCTGCT GCATGTGCAA GGCAGACCAT GGTACCACCT 
CCTACTTCTC AGGCCATGAA TCATCTTTTA GTGGGCAAAA GTGCGCTCGT GAGTGGGAAG 
AATCGACCCC TGAGCCTACC TTCTGGTGCT GCAACGACGA AGATAAGACC TACGACGCTA 
AATCAAAACC GAAGGGACAG ACAAGGCGTC ACGCTTCACT GGAAAGACAC CAAAAAACCG 
AAGCAGAAAC CCTCGAATTT ACCAGAAACG GTGGAATGTA GCGTTGTAAC AATGCAATCC 
ACGGAAGCTG AGCTGGCTTC TCCAAAGCGG AGTAACGGTG ACAGTGATAG CGACAGCACA 
TCGACGGAAT TGTGCGACGA ACCGGACCGT CTCAGTGACG TTGACAGCGA AGATCTGACG 
TCAGCATCCG AATCCTGTGT TGTCAGTACG GATGAGGAGA AAAGCTCTCG AATCACCGGA 
TCACCGATTC GCGAGAAGCC ACTCGATCGT TCGTTTCTCG AAGAGAAGAT GGAACCAATT 
AACGCCGAAG ATTCGAAGGA CTCGAAAAGC TCCGAGGATA ACCTTGGTGA CATATCAATC 
GCTAAAATCA CCGACCAAAT AAGACGATTA TCAGCAGAGG ATGACAATAA TTCCATGGTT 
CCTCAAAAAT TTAGCGTGCA GAGCAAAGAA TCGCCGGAAG AACAATCGAC GAAAGAATCG 
AAAAAAGAGA AATCCATATC AGATCTATCT ATAGATTACA CTTCCGATAG TAATATCATT 
AAAAAATCGT TATTGGACTT GAACGAGTAC GATTATCTAG GTTTCAGTGG CAGTAAGATC 
AGTTCGAAAG AAGATGAAGT ATTAGTGCAC GAAAGGCATC GATCTAAACA GACAACAAAC 
GAGATTGTAG ATATACCTCG AAACGAAGTT GCGACAATCA AGAAAGACCA AACTGAAGAC 
AAGATAACTA CGACTATGAA ACAGGATTCT TTAAACATAT ACTTGGATTA TTCAGCATCC 
TTGGACAAGA AATACGATAG AATCAAAGAT AAATCAAGCC TCGATGTAGA GATAGAAAAT 
GAAATTAGAT CCGACCGATA TAAAACATCC TTCGATTTCT CTAGTAGAAT ATCTTTGGAC 
TTGAAACCGT ATGAAACTTC AAAAGTAGGA AGTTTAACCA GTGACACTGC AGACACAGTG 
AATACTGAAA GAACGCTTTA TGGCAAGGCC TACGTTGGTC ATCTTCCTAT TTCACCTGTA 
ACAGTTGATC AAAAATTAAG CAGAGTGACA CCCAGTATAC ATACAACGCA TAATACTACT 
GTATTAACTC CTGAAACGCC TGAAATTGTA CATAAAACGT TAGAGAACAC GTCATATAAC 
GATAGAATAC AGACGACTCA AACGAAAATT TATTCAGACC TAGTGAAGAG TCCTAAAACA 
CCGGAAAGCA ACAAATCATT CAGTTCAGGT GAAACCATGG GACCAGATTC GAAACCATCG 
AGTCTGACAG TTAGCCCAAG GACGCCGGTT AGATCAGAAT CTCGAGATTT CCCTATGGAC 
AAATCCCTGT GTTCGTCAGT TAGCTCAGAC TCGAAGACTT TGGTTCTTCA TTCTGCAGAT 
TCAGACAGTA CGAGGGACAG CACGAAAACC GACGCGAAGA AAATGTATGA GATTTCCAAT 
TCGGCGACAC CTATCAGCAT GGACTCGTTG CAGAAATCAG AAGCCAGCCC AAAACTAGTC 
GTGAGCAGCT CCAGTGAATC GTGTAGCCCG AGTAAATTAA AGTCTTCCGA GAGGTCGTTC 
AGTTCGGACC TGCAATCGCC AATAAGTGCC AACTCCATAA AGTATACTTC GAATTTCAGT 
AGACGGGGCC AAGATGACGT ATCAGGCTCG CCAATGGACT TGAGCAGCGC TACCTCGCCG 
TTTTATCCGA TCTCGAATCC GAATCAAAAA ACTCCGACGT CCCCGTACAG TGCTTCTAGA 
AGAAGATCCA GTTTGGACAG CAACGAAACG TCACCTGAGC AGAAATCGAC CAGTTCGAGG 
GAGTCGAACA AGTTTCTAGG TTATCAGTCG AAATTTGACT CCTCCATAAA GACGTCTGAT 
ATGAAAGATG TAGACGGTAT AGCACGAAAA GGTGACACAT TCGTCAGACC AGAGTTCATC 
ACGAGGAATG AAGATTCGAG TATTAATTTG ACGGATAGAA GAATCAATGT TAGTGATGTT 
AAGTATTATC AAACAACGAG TACGGATTAC TATCGCAAGG ATAAGGATAT TTTTGATGAC 
GGAGGGGATG ATCCGTCATC GGAGAAAGAC GTGGTACCTT CTTTGCCTCA GGAGAAGCTA 
GATAAACATT ATTTGAACTA TCGATATAGA GATAGATCGA AGTTGTTGGA AAGAAGTTCA 
AGCAGTTTGG ACAGTAGAAG GTACGCTGAT ATGAAGTCAT CGGCTTCCAC CGATGAATTT 
TCAACGTCGA TCGCGCAGAA GAGATCCAGT GACATTTTAG AAGATATTAA ACACTTGGAG 
GCGAAAGCAA ACGATGGGTC GTCGGATAAT GGGATGTTGA CGAAGAAGAG CAGTTATATT 
TGGGAGGATA TGAAGAATTT AGAAGCCAGG AGGCAAGATA CCTTTAGTGA TTCAGCGAGG 
AACTTTTCGA AACATCGTCT GGAGGTACCG GATATAAATA TAACCGGAGA AAGCAGAAAA 
TCGTACGTAG TACATCCGAA GATCAATATC GACGAAAATA GCGACAGCAT ATTGAAGACC 
ATGGCCTGTG GTAAGAGTAA CGGCACAGAT GATGGCAGGG AGTCGAAGTT GGGCGTGTGG 
ACGAAGGTGA AGCCTCGTAA AAAGAGTGAC AATGGGCGGA GGAACAGCGA CAGGGCGTTG 
AAGATCATTC AAGAAAACTC CGCTATACTT CAGAAGATTC TTGCGTGTCA GGCAAAAAAG 
CGTCTGCCAG ACCTGGAGGA GATATCGAAA GAAATTACCA TTAGCCCTAT AAACGAGGAG 
ATTTCGAAGA TCTTTAGTCC GATATTAGAG AAAATGGGAT TAAACGAACA CGAGATTAAC 
GAGGAATTGG CGAGAATCAA TTTCAAGGAT TTCGACAACA TGACCGCTAC CAGTGTGTCC 
GAGTTTGATG CAAAGATTAA CGAGGAATTA TCGAAGCTTT CTCTGATCGA CGAGACGGAG 
CAGATGGACC ACTTCGACGT GGACGAGGTG ATATCCCACG AATACTTGAA CACGAGAGAA 
GCTCTAATAG ATCGTAAGAT AAACGAGGAA TTGTCGAAAT TATTGGCAAA CTACGACGAT 
CATTCTCCGG CTAGTATGGT GAGTTTGGAC AAAGGACCAT CCAGCCAGAA TATTAGCGAG 
ATCGAAGGAC TGGATCTGTC CAGTATTTCA ACTAACGTAT TTTCCTATAA ATCATCCAAT 
GACTCCATTG ACACGAGAAG CGACTTAGGC TCGACGCAGG AACCATCCAT TCTCGTTGAC 
AAGTTTCCGA AATATACGGC ATACGCCGTA CCGAAGTATC ACGTGGACGA CTCATCACCT 
AAAAGCGACA TAGACATCTA CAGAGAATTG GAGAAACTCG ATAAGATCTC TTCCGCACAG 
GTGTTACCAG ATCCGACTCT GGAGCTTCCA CAGAAGGAAT TGTCCTCGAT TCCGTACGTT 
CCAAATACGT CGCCCTTCAA GGATGTGTCA CCATTGAGGT ACACTTCCAA GCCGGCTCAA 
TACGACACTT CACCCCTGAA GACTTCTTAC GATCCCTACA AGACCTTTGA CTTCAAGCCC 
AAGTTGTCAC CTAAACAAGT TTCCACTAAT CCATTTGTGT CAGCTACGTA TGAGAAACCT 
GTGGTGGATA CCGCTTTCGA GTACATTAAC AATAAACCAG AGATCTCGAT CAATACGTAT 
GATCTACATT TGAAGAGTCC AATGATGACG AAGGAATCGC TAGAGTTTCG TGTCAGATAC 
GACGATGATC CAGTCAGAGA AGTTAACACC GATTACATGT CTCTCCAGCC AGAAAGTAGA 
TCGATTACTA CCCCGAGCAA GTCTCCTTAT TTCAGCAATG GAAATACCGA ACCGTTAACG 
CCTACGAAAT ATATTTCTAA GGAGGAAAGA ATCTTGGATA CTGGTGGTAC CTCGTTTCTG 
AACTATCCCA GCGAATCTTC CGACCTTCTT CGTCGAAAAT ACGACGCTTT GTCTCCTAAG 
GAGTACACTC ATACCAAGAG TACGACGGAT TATGATAGAC ACTTAGGCAC GTTACCTCCT 
ATAGAACCTG GAACAGAGAC AGGATCGTAT TTACCGACGA GACACCGAGA TTATCATTCT 
CTATCACCGA ATCGTCAGTT TCCTGATCAC TTTCCTTCTA CTATCAATCA TCACTACACC 
TCGAAACTTT CATCAGGTCT CTCTGACGAA TCGAAACGAC CGGTTTCTCA TCCGGAATTC 
CCTGGCAAGA TGAATGTTGG GAAATATGCA GAATTACCCG ATAGAAGTTC CAGCAGTTAC 
AAGAAATCAT CTCTTAGCTT AGGTAACATA GGACACTCGA GTAAAGGAGC TGATAATAAT 
TTCAACACTG TGACCAGTCC AAAAACGCAG TTCAGTCCCT TTCCCGTTAG AAACGCTCCT 
AGGAAGCCGA AAGAACTTAC GCTGAAATTG GGCCTCTATT CGCCCAAAGG TTCTGATACA 
GGACAGTTAA AAAGGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024224634.1
CDS	458..6418
Translation	MSGFFNSLKNFASGAAYAASSASKYEELEVEDTKMRFSLTPQPGESGFIQWLDAMKMVARLPGGIPPEFRKKLWLTLAERHLEQRGVDWKQAEKICFNEWSNPDDEELGIQIVKDLHRTGCSLFCGAAGRDNQAVLRRVLLGFARWNKSVGYCQGLNVLAALVLQVMDRAESAAVKVMIYLIEGVLPEGYFADNLRGLSVDMAVFRDLLRGRLPKLSKHLEALQNDAKDKATGSSYEPPLTNVFTMQWFLTLFCHCLPQEAVLRVWDLIFLEGDEILLRTALAIWEGLSDRIMTVTSADEFYSIMGVLTREMLEFTDTNNLIKNIVSMGSLHGVTGLREKHRYNITPWARKLSDDEDSDTEEDERLAVAAAMFSMAQRLKKDRIPQTIGALQAMAPSSDRERLALDITTLKQQYAKLRERQRQAHIILSAACARQTMVPPPTSQAMNHLLVGKSALVSGKNRPLSLPSGAATTKIRPTTLNQNRRDRQGVTLHWKDTKKPKQKPSNLPETVECSVVTMQSTEAELASPKRSNGDSDSDSTSTELCDEPDRLSDVDSEDLTSASESCVVSTDEEKSSRITGSPIREKPLDRSFLEEKMEPINAEDSKDSKSSEDNLGDISIAKITDQIRRLSAEDDNNSMVPQKFSVQSKESPEEQSTKESKKEKSISDLSIDYTSDSNIIKKSLLDLNEYDYLGFSGSKISSKEDEVLVHERHRSKQTTNEIVDIPRNEVATIKKDQTEDKITTTMKQDSLNIYLDYSASLDKKYDRIKDKSSLDVEIENEIRSDRYKTSFDFSSRISLDLKPYETSKVGSLTSDTADTVNTERTLYGKAYVGHLPISPVTVDQKLSRVTPSIHTTHNTTVLTPETPEIVHKTLENTSYNDRIQTTQTKIYSDLVKSPKTPESNKSFSSGETMGPDSKPSSLTVSPRTPVRSESRDFPMDKSLCSSVSSDSKTLVLHSADSDSTRDSTKTDAKKMYEISNSATPISMDSLQKSEASPKLVVSSSSESCSPSKLKSSERSFSSDLQSPISANSIKYTSNFSRRGQDDVSGSPMDLSSATSPFYPISNPNQKTPTSPYSASRRRSSLDSNETSPEQKSTSSRESNKFLGYQSKFDSSIKTSDMKDVDGIARKGDTFVRPEFITRNEDSSINLTDRRINVSDVKYYQTTSTDYYRKDKDIFDDGGDDPSSEKDVVPSLPQEKLDKHYLNYRYRDRSKLLERSSSSLDSRRYADMKSSASTDEFSTSIAQKRSSDILEDIKHLEAKANDGSSDNGMLTKKSSYIWEDMKNLEARRQDTFSDSARNFSKHRLEVPDINITGESRKSYVVHPKINIDENSDSILKTMACGKSNGTDDGRESKLGVWTKVKPRKKSDNGRRNSDRALKIIQENSAILQKILACQAKKRLPDLEEISKEITISPINEEISKIFSPILEKMGLNEHEINEELARINFKDFDNMTATSVSEFDAKINEELSKLSLIDETEQMDHFDVDEVISHEYLNTREALIDRKINEELSKLLANYDDHSPASMVSLDKGPSSQNISEIEGLDLSSISTNVFSYKSSNDSIDTRSDLGSTQEPSILVDKFPKYTAYAVPKYHVDDSSPKSDIDIYRELEKLDKISSAQVLPDPTLELPQKELSSIPYVPNTSPFKDVSPLRYTSKPAQYDTSPLKTSYDPYKTFDFKPKLSPKQVSTNPFVSATYEKPVVDTAFEYINNKPEISINTYDLHLKSPMMTKESLEFRVRYDDDPVREVNTDYMSLQPESRSITTPSKSPYFSNGNTEPLTPTKYISKEERILDTGGTSFLNYPSESSDLLRRKYDALSPKEYTHTKSTTDYDRHLGTLPPIEPGTETGSYLPTRHRDYHSLSPNRQFPDHFPSTINHHYTSKLSSGLSDESKRPVSHPEFPGKMNVGKYAELPDRSSSSYKKSSLSLGNIGHSSKGADNNFNTVTSPKTQFSPFPVRNAPRKPKELTLKLGLYSPKGSDTGQLKRS

Target ORF information:

RefSeq Version	XM_024368866.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024368866.1

ORF Insert Sequence:

ATGAGCGGAT TTTTCAACTC CCTGAAAAAC TTCGCGTCAG GAGCCGCGTA CGCTGCCAGC 
TCTGCCTCGA AGTACGAAGA ATTGGAAGTC GAAGACACGA AGATGAGATT TAGCCTGACG 
CCGCAACCAG GAGAAAGTGG TTTTATACAA TGGCTGGACG CGATGAAAAT GGTTGCCAGG 
TTACCAGGAG GAATTCCGCC GGAATTTCGA AAGAAGTTGT GGCTGACACT GGCAGAACGT 
CATCTAGAGC AGCGCGGCGT AGATTGGAAG CAGGCTGAAA AGATCTGCTT CAACGAGTGG 
AGCAATCCTG ACGATGAAGA GCTTGGTATA CAAATCGTTA AAGACCTACA CAGAACGGGC 
TGCAGTCTGT TTTGCGGTGC AGCTGGCAGA GACAATCAAG CAGTGCTTCG TCGAGTTCTG 
CTTGGTTTCG CCCGATGGAA CAAGTCCGTC GGCTATTGCC AGGGCCTGAA CGTGTTGGCC 
GCCCTCGTTT TGCAAGTAAT GGATCGCGCC GAGTCCGCCG CGGTGAAGGT GATGATCTAC 
TTGATAGAGG GTGTCCTGCC CGAGGGTTAC TTCGCGGACA ATCTCCGTGG CCTGTCGGTC 
GACATGGCGG TATTTCGAGA TCTCCTCCGG GGCAGACTGC CCAAGCTCTC GAAACATCTG 
GAGGCTCTGC AGAACGATGC TAAGGACAAG GCGACAGGCA GCAGTTACGA GCCACCTCTG 
ACGAATGTGT TCACGATGCA ATGGTTCCTT ACTCTTTTCT GTCACTGTTT GCCACAAGAG 
GCTGTCCTGC GCGTATGGGA CCTCATATTT CTCGAAGGAG ATGAGATATT GCTTAGAACC 
GCACTGGCCA TCTGGGAAGG ACTTTCCGAT CGTATTATGA CGGTGACATC GGCGGACGAG 
TTTTATAGTA TAATGGGAGT GCTGACAAGG GAGATGCTCG AGTTCACGGA TACTAATAAC 
CTCATAAAGA ATATCGTTAG TATGGGATCT TTGCATGGTG TAACGGGGCT AAGAGAGAAA 
CATAGATACA ATATCACACC TTGGGCAAGA AAATTGAGCG ACGACGAGGA CAGCGACACG 
GAGGAGGATG AAAGGCTGGC CGTGGCTGCT GCGATGTTCA GTATGGCACA GCGATTGAAG 
AAAGATCGTA TACCTCAAAC GATTGGAGCG CTGCAGGCAA TGGCACCCAG CAGCGACCGA 
GAACGGCTCG CTTTGGACAT TACCACTTTG AAACAGCAGT ACGCCAAGCT ACGCGAACGT 
CAACGACAAG CGCATATTAT ACTTTCTGCT GCATGTGCAA GGCAGACCAT GGTACCACCT 
CCTACTTCTC AGGCCATGAA TCATCTTTTA GTGGGCAAAA GTGCGCTCGT GAGTGGGAAG 
AATCGACCCC TGAGCCTACC TTCTGGTGCT GCAACGACGA AGATAAGACC TACGACGCTA 
AATCAAAACC GAAGGGACAG ACAAGGCGTC ACGCTTCACT GGAAAGACAC CAAAAAACCG 
AAGCAGAAAC CCTCGAATTT ACCAGAAACG GTGGAATGTA GCGTTGTAAC AATGCAATCC 
ACGGAAGCTG AGCTGGCTTC TCCAAAGCGG AGTAACGGTG ACAGTGATAG CGACAGCACA 
TCGACGGAAT TGTGCGACGA ACCGGACCGT CTCAGTGACG TTGACAGCGA AGATCTGACG 
TCAGCATCCG AATCCTGTGT TGTCAGTACG GATGAGGAGA AAAGCTCTCG AATCACCGGA 
TCACCGATTC GCGAGAAGCC ACTCGATCGT TCGTTTCTCG AAGAGAAGAT GGAACCAATT 
AACGCCGAAG ATTCGAAGGA CTCGAAAAGC TCCGAGGATA ACCTTGGTGA CATATCAATC 
GCTAAAATCA CCGACCAAAT AAGACGATTA TCAGCAGAGG ATGACAATAA TTCCATGGTT 
CCTCAAAAAT TTAGCGTGCA GAGCAAAGAA TCGCCGGAAG AACAATCGAC GAAAGAATCG 
AAAAAAGAGA AATCCATATC AGATCTATCT ATAGATTACA CTTCCGATAG TAATATCATT 
AAAAAATCGT TATTGGACTT GAACGAGTAC GATTATCTAG GTTTCAGTGG CAGTAAGATC 
AGTTCGAAAG AAGATGAAGT ATTAGTGCAC GAAAGGCATC GATCTAAACA GACAACAAAC 
GAGATTGTAG ATATACCTCG AAACGAAGTT GCGACAATCA AGAAAGACCA AACTGAAGAC 
AAGATAACTA CGACTATGAA ACAGGATTCT TTAAACATAT ACTTGGATTA TTCAGCATCC 
TTGGACAAGA AATACGATAG AATCAAAGAT AAATCAAGCC TCGATGTAGA GATAGAAAAT 
GAAATTAGAT CCGACCGATA TAAAACATCC TTCGATTTCT CTAGTAGAAT ATCTTTGGAC 
TTGAAACCGT ATGAAACTTC AAAAGTAGGA AGTTTAACCA GTGACACTGC AGACACAGTG 
AATACTGAAA GAACGCTTTA TGGCAAGGCC TACGTTGGTC ATCTTCCTAT TTCACCTGTA 
ACAGTTGATC AAAAATTAAG CAGAGTGACA CCCAGTATAC ATACAACGCA TAATACTACT 
GTATTAACTC CTGAAACGCC TGAAATTGTA CATAAAACGT TAGAGAACAC GTCATATAAC 
GATAGAATAC AGACGACTCA AACGAAAATT TATTCAGACC TAGTGAAGAG TCCTAAAACA 
CCGGAAAGCA ACAAATCATT CAGTTCAGGT GAAACCATGG GACCAGATTC GAAACCATCG 
AGTCTGACAG TTAGCCCAAG GACGCCGGTT AGATCAGAAT CTCGAGATTT CCCTATGGAC 
AAATCCCTGT GTTCGTCAGT TAGCTCAGAC TCGAAGACTT TGGTTCTTCA TTCTGCAGAT 
TCAGACAGTA CGAGGGACAG CACGAAAACC GACGCGAAGA AAATGTATGA GATTTCCAAT 
TCGGCGACAC CTATCAGCAT GGACTCGTTG CAGAAATCAG AAGCCAGCCC AAAACTAGTC 
GTGAGCAGCT CCAGTGAATC GTGTAGCCCG AGTAAATTAA AGTCTTCCGA GAGGTCGTTC 
AGTTCGGACC TGCAATCGCC AATAAGTGCC AACTCCATAA AGTATACTTC GAATTTCAGT 
AGACGGGGCC AAGATGACGT ATCAGGCTCG CCAATGGACT TGAGCAGCGC TACCTCGCCG 
TTTTATCCGA TCTCGAATCC GAATCAAAAA ACTCCGACGT CCCCGTACAG TGCTTCTAGA 
AGAAGATCCA GTTTGGACAG CAACGAAACG TCACCTGAGC AGAAATCGAC CAGTTCGAGG 
GAGTCGAACA AGTTTCTAGG TTATCAGTCG AAATTTGACT CCTCCATAAA GACGTCTGAT 
ATGAAAGATG TAGACGGTAT AGCACGAAAA GGTGACACAT TCGTCAGACC AGAGTTCATC 
ACGAGGAATG AAGATTCGAG TATTAATTTG ACGGATAGAA GAATCAATGT TAGTGATGTT 
AAGTATTATC AAACAACGAG TACGGATTAC TATCGCAAGG ATAAGGATAT TTTTGATGAC 
GGAGGGGATG ATCCGTCATC GGAGAAAGAC GTGGTACCTT CTTTGCCTCA GGAGAAGCTA 
GATAAACATT ATTTGAACTA TCGATATAGA GATAGATCGA AGTTGTTGGA AAGAAGTTCA 
AGCAGTTTGG ACAGTAGAAG GTACGCTGAT ATGAAGTCAT CGGCTTCCAC CGATGAATTT 
TCAACGTCGA TCGCGCAGAA GAGATCCAGT GACATTTTAG AAGATATTAA ACACTTGGAG 
GCGAAAGCAA ACGATGGGTC GTCGGATAAT GGGATGTTGA CGAAGAAGAG CAGTTATATT 
TGGGAGGATA TGAAGAATTT AGAAGCCAGG AGGCAAGATA CCTTTAGTGA TTCAGCGAGG 
AACTTTTCGA AACATCGTCT GGAGGTACCG GATATAAATA TAACCGGAGA AAGCAGAAAA 
TCGTACGTAG TACATCCGAA GATCAATATC GACGAAAATA GCGACAGCAT ATTGAAGACC 
ATGGCCTGTG GTAAGAGTAA CGGCACAGAT GATGGCAGGG AGTCGAAGTT GGGCGTGTGG 
ACGAAGGTGA AGCCTCGTAA AAAGAGTGAC AATGGGCGGA GGAACAGCGA CAGGGCGTTG 
AAGATCATTC AAGAAAACTC CGCTATACTT CAGAAGATTC TTGCGTGTCA GGCAAAAAAG 
CGTCTGCCAG ACCTGGAGGA GATATCGAAA GAAATTACCA TTAGCCCTAT AAACGAGGAG 
ATTTCGAAGA TCTTTAGTCC GATATTAGAG AAAATGGGAT TAAACGAACA CGAGATTAAC 
GAGGAATTGG CGAGAATCAA TTTCAAGGAT TTCGACAACA TGACCGCTAC CAGTGTGTCC 
GAGTTTGATG CAAAGATTAA CGAGGAATTA TCGAAGCTTT CTCTGATCGA CGAGACGGAG 
CAGATGGACC ACTTCGACGT GGACGAGGTG ATATCCCACG AATACTTGAA CACGAGAGAA 
GCTCTAATAG ATCGTAAGAT AAACGAGGAA TTGTCGAAAT TATTGGCAAA CTACGACGAT 
CATTCTCCGG CTAGTATGGT GAGTTTGGAC AAAGGACCAT CCAGCCAGAA TATTAGCGAG 
ATCGAAGGAC TGGATCTGTC CAGTATTTCA ACTAACGTAT TTTCCTATAA ATCATCCAAT 
GACTCCATTG ACACGAGAAG CGACTTAGGC TCGACGCAGG AACCATCCAT TCTCGTTGAC 
AAGTTTCCGA AATATACGGC ATACGCCGTA CCGAAGTATC ACGTGGACGA CTCATCACCT 
AAAAGCGACA TAGACATCTA CAGAGAATTG GAGAAACTCG ATAAGATCTC TTCCGCACAG 
GTGTTACCAG ATCCGACTCT GGAGCTTCCA CAGAAGGAAT TGTCCTCGAT TCCGTACGTT 
CCAAATACGT CGCCCTTCAA GGATGTGTCA CCATTGAGGT ACACTTCCAA GCCGGCTCAA 
TACGACACTT CACCCCTGAA GACTTCTTAC GATCCCTACA AGACCTTTGA CTTCAAGCCC 
AAGTTGTCAC CTAAACAAGT TTCCACTAAT CCATTTGTGT CAGCTACGTA TGAGAAACCT 
GTGGTGGATA CCGCTTTCGA GTACATTAAC AATAAACCAG AGATCTCGAT CAATACGTAT 
GATCTACATT TGAAGAGTCC AATGATGACG AAGGAATCGC TAGAGTTTCG TGTCAGATAC 
GACGATGATC CAGTCAGAGA AGTTAACACC GATTACATGT CTCTCCAGCC AGAAAGTAGA 
TCGATTACTA CCCCGAGCAA GTCTCCTTAT TTCAGCAATG GAAATACCGA ACCGTTAACG 
CCTACGAAAT ATATTTCTAA GGAGGAAAGA ATCTTGGATA CTGGTGGTAC CTCGTTTCTG 
AACTATCCCA GCGAATCTTC CGACCTTCTT CGTCGAAAAT ACGACGCTTT GTCTCCTAAG 
GAGTACACTC ATACCAAGAG TACGACGGAT TATGATAGAC ACTTAGGCAC GTTACCTCCT 
ATAGAACCTG GAACAGAGAC AGGATCGTAT TTACCGACGA GACACCGAGA TTATCATTCT 
CTATCACCGA ATCGTCAGTT TCCTGATCAC TTTCCTTCTA CTATCAATCA TCACTACACC 
TCGAAACTTT CATCAGGTCT CTCTGACGAA TCGAAACGAC CGGTTTCTCA TCCGGAATTC 
CCTGGCAAGA TGAATGTTGG GAAATATGCA GAATTACCCG ATAGAAGTTC CAGCAGTTAC 
AAGAAATCAT CTCTTAGCTT AGGTAACATA GGACACTCGA GTAAAGGAGC TGATAATAAT 
TTCAACACTG TGACCAGTCC AAAAACGCAG TTCAGTCCCT TTCCCGTTAG AAACGCTCCT 
AGGAAGCCGA AAGAACTTAC GCTGAAATTG GGCCTCTATT CGCCCAAAGG TTCTGATACA 
GGACAGTTAA AAAGGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100749006 ( XM_012387845.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012243268.1 Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X2, mRNA.

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CloneID	OBo106113
Clone ID Related Accession (Same CDS sequence)	XM_012387845.2 , XM_024368866.1 , XM_012387845.1 , XM_012387846.1
Accession Version	XM_012387845.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	uncharacterized protein LOC100749006 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176906.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGAT TTTTCAACTC CCTGAAAAAC TTCGCGTCAG GAGCCGCGTA CGCTGCCAGC 
TCTGCCTCGA AGTACGAAGA ATTGGAAGTC GAAGACACGA AGATGAGATT TAGCCTGACG 
CCGCAACCAG GAGAAAGTGG TTTTATACAA TGGCTGGACG CGATGAAAAT GGTTGCCAGG 
TTACCAGGAG GAATTCCGCC GGAATTTCGA AAGAAGTTGT GGCTGACACT GGCAGAACGT 
CATCTAGAGC AGCGCGGCGT AGATTGGAAG CAGGCTGAAA AGATCTGCTT CAACGAGTGG 
AGCAATCCTG ACGATGAAGA GCTTGGTATA CAAATCGTTA AAGACCTACA CAGAACGGGC 
TGCAGTCTGT TTTGCGGTGC AGCTGGCAGA GACAATCAAG CAGTGCTTCG TCGAGTTCTG 
CTTGGTTTCG CCCGATGGAA CAAGTCCGTC GGCTATTGCC AGGGCCTGAA CGTGTTGGCC 
GCCCTCGTTT TGCAAGTAAT GGATCGCGCC GAGTCCGCCG CGGTGAAGGT GATGATCTAC 
TTGATAGAGG GTGTCCTGCC CGAGGGTTAC TTCGCGGACA ATCTCCGTGG CCTGTCGGTC 
GACATGGCGG TATTTCGAGA TCTCCTCCGG GGCAGACTGC CCAAGCTCTC GAAACATCTG 
GAGGCTCTGC AGAACGATGC TAAGGACAAG GCGACAGGCA GCAGTTACGA GCCACCTCTG 
ACGAATGTGT TCACGATGCA ATGGTTCCTT ACTCTTTTCT GTCACTGTTT GCCACAAGAG 
GCTGTCCTGC GCGTATGGGA CCTCATATTT CTCGAAGGAG ATGAGATATT GCTTAGAACC 
GCACTGGCCA TCTGGGAAGG ACTTTCCGAT CGTATTATGA CGGTGACATC GGCGGACGAG 
TTTTATAGTA TAATGGGAGT GCTGACAAGG GAGATGCTCG AGTTCACGGA TACTAATAAC 
CTCATAAAGA ATATCGTTAG TATGGGATCT TTGCATGGTG TAACGGGGCT AAGAGAGAAA 
CATAGATACA ATATCACACC TTGGGCAAGA AAATTGAGCG ACGACGAGGA CAGCGACACG 
GAGGAGGATG AAAGGCTGGC CGTGGCTGCT GCGATGTTCA GTATGGCACA GCGATTGAAG 
AAAGATCGTA TACCTCAAAC GATTGGAGCG CTGCAGGCAA TGGCACCCAG CAGCGACCGA 
GAACGGCTCG CTTTGGACAT TACCACTTTG AAACAGCAGT ACGCCAAGCT ACGCGAACGT 
CAACGACAAG CGCATATTAT ACTTTCTGCT GCATGTGCAA GGCAGACCAT GGTACCACCT 
CCTACTTCTC AGGCCATGAA TCATCTTTTA GTGGGCAAAA GTGCGCTCGT GAGTGGGAAG 
AATCGACCCC TGAGCCTACC TTCTGGTGCT GCAACGACGA AGATAAGACC TACGACGCTA 
AATCAAAACC GAAGGGACAG ACAAGGCGTC ACGCTTCACT GGAAAGACAC CAAAAAACCG 
AAGCAGAAAC CCTCGAATTT ACCAGAAACG GTGGAATGTA GCGTTGTAAC AATGCAATCC 
ACGGAAGCTG AGCTGGCTTC TCCAAAGCGG AGTAACGGTG ACAGTGATAG CGACAGCACA 
TCGACGGAAT TGTGCGACGA ACCGGACCGT CTCAGTGACG TTGACAGCGA AGATCTGACG 
TCAGCATCCG AATCCTGTGT TGTCAGTACG GATGAGGAGA AAAGCTCTCG AATCACCGGA 
TCACCGATTC GCGAGAAGCC ACTCGATCGT TCGTTTCTCG AAGAGAAGAT GGAACCAATT 
AACGCCGAAG ATTCGAAGGA CTCGAAAAGC TCCGAGGATA ACCTTGGTGA CATATCAATC 
GCTAAAATCA CCGACCAAAT AAGACGATTA TCAGCAGAGG ATGACAATAA TTCCATGGTT 
CCTCAAAAAT TTAGCGTGCA GAGCAAAGAA TCGCCGGAAG AACAATCGAC GAAAGAATCG 
AAAAAAGAGA AATCCATATC AGATCTATCT ATAGATTACA CTTCCGATAG TAATATCATT 
AAAAAATCGT TATTGGACTT GAACGAGTAC GATTATCTAG GTTTCAGTGG CAGTAAGATC 
AGTTCGAAAG AAGATGAAGT ATTAGTGCAC GAAAGGCATC GATCTAAACA GACAACAAAC 
GAGATTGTAG ATATACCTCG AAACGAAGTT GCGACAATCA AGAAAGACCA AACTGAAGAC 
AAGATAACTA CGACTATGAA ACAGGATTCT TTAAACATAT ACTTGGATTA TTCAGCATCC 
TTGGACAAGA AATACGATAG AATCAAAGAT AAATCAAGCC TCGATGTAGA GATAGAAAAT 
GAAATTAGAT CCGACCGATA TAAAACATCC TTCGATTTCT CTAGTAGAAT ATCTTTGGAC 
TTGAAACCGT ATGAAACTTC AAAAGTAGGA AGTTTAACCA GTGACACTGC AGACACAGTG 
AATACTGAAA GAACGCTTTA TGGCAAGGCC TACGTTGGTC ATCTTCCTAT TTCACCTGTA 
ACAGTTGATC AAAAATTAAG CAGAGTGACA CCCAGTATAC ATACAACGCA TAATACTACT 
GTATTAACTC CTGAAACGCC TGAAATTGTA CATAAAACGT TAGAGAACAC GTCATATAAC 
GATAGAATAC AGACGACTCA AACGAAAATT TATTCAGACC TAGTGAAGAG TCCTAAAACA 
CCGGAAAGCA ACAAATCATT CAGTTCAGGT GAAACCATGG GACCAGATTC GAAACCATCG 
AGTCTGACAG TTAGCCCAAG GACGCCGGTT AGATCAGAAT CTCGAGATTT CCCTATGGAC 
AAATCCCTGT GTTCGTCAGT TAGCTCAGAC TCGAAGACTT TGGTTCTTCA TTCTGCAGAT 
TCAGACAGTA CGAGGGACAG CACGAAAACC GACGCGAAGA AAATGTATGA GATTTCCAAT 
TCGGCGACAC CTATCAGCAT GGACTCGTTG CAGAAATCAG AAGCCAGCCC AAAACTAGTC 
GTGAGCAGCT CCAGTGAATC GTGTAGCCCG AGTAAATTAA AGTCTTCCGA GAGGTCGTTC 
AGTTCGGACC TGCAATCGCC AATAAGTGCC AACTCCATAA AGTATACTTC GAATTTCAGT 
AGACGGGGCC AAGATGACGT ATCAGGCTCG CCAATGGACT TGAGCAGCGC TACCTCGCCG 
TTTTATCCGA TCTCGAATCC GAATCAAAAA ACTCCGACGT CCCCGTACAG TGCTTCTAGA 
AGAAGATCCA GTTTGGACAG CAACGAAACG TCACCTGAGC AGAAATCGAC CAGTTCGAGG 
GAGTCGAACA AGTTTCTAGG TTATCAGTCG AAATTTGACT CCTCCATAAA GACGTCTGAT 
ATGAAAGATG TAGACGGTAT AGCACGAAAA GGTGACACAT TCGTCAGACC AGAGTTCATC 
ACGAGGAATG AAGATTCGAG TATTAATTTG ACGGATAGAA GAATCAATGT TAGTGATGTT 
AAGTATTATC AAACAACGAG TACGGATTAC TATCGCAAGG ATAAGGATAT TTTTGATGAC 
GGAGGGGATG ATCCGTCATC GGAGAAAGAC GTGGTACCTT CTTTGCCTCA GGAGAAGCTA 
GATAAACATT ATTTGAACTA TCGATATAGA GATAGATCGA AGTTGTTGGA AAGAAGTTCA 
AGCAGTTTGG ACAGTAGAAG GTACGCTGAT ATGAAGTCAT CGGCTTCCAC CGATGAATTT 
TCAACGTCGA TCGCGCAGAA GAGATCCAGT GACATTTTAG AAGATATTAA ACACTTGGAG 
GCGAAAGCAA ACGATGGGTC GTCGGATAAT GGGATGTTGA CGAAGAAGAG CAGTTATATT 
TGGGAGGATA TGAAGAATTT AGAAGCCAGG AGGCAAGATA CCTTTAGTGA TTCAGCGAGG 
AACTTTTCGA AACATCGTCT GGAGGTACCG GATATAAATA TAACCGGAGA AAGCAGAAAA 
TCGTACGTAG TACATCCGAA GATCAATATC GACGAAAATA GCGACAGCAT ATTGAAGACC 
ATGGCCTGTG GTAAGAGTAA CGGCACAGAT GATGGCAGGG AGTCGAAGTT GGGCGTGTGG 
ACGAAGGTGA AGCCTCGTAA AAAGAGTGAC AATGGGCGGA GGAACAGCGA CAGGGCGTTG 
AAGATCATTC AAGAAAACTC CGCTATACTT CAGAAGATTC TTGCGTGTCA GGCAAAAAAG 
CGTCTGCCAG ACCTGGAGGA GATATCGAAA GAAATTACCA TTAGCCCTAT AAACGAGGAG 
ATTTCGAAGA TCTTTAGTCC GATATTAGAG AAAATGGGAT TAAACGAACA CGAGATTAAC 
GAGGAATTGG CGAGAATCAA TTTCAAGGAT TTCGACAACA TGACCGCTAC CAGTGTGTCC 
GAGTTTGATG CAAAGATTAA CGAGGAATTA TCGAAGCTTT CTCTGATCGA CGAGACGGAG 
CAGATGGACC ACTTCGACGT GGACGAGGTG ATATCCCACG AATACTTGAA CACGAGAGAA 
GCTCTAATAG ATCGTAAGAT AAACGAGGAA TTGTCGAAAT TATTGGCAAA CTACGACGAT 
CATTCTCCGG CTAGTATGGT GAGTTTGGAC AAAGGACCAT CCAGCCAGAA TATTAGCGAG 
ATCGAAGGAC TGGATCTGTC CAGTATTTCA ACTAACGTAT TTTCCTATAA ATCATCCAAT 
GACTCCATTG ACACGAGAAG CGACTTAGGC TCGACGCAGG AACCATCCAT TCTCGTTGAC 
AAGTTTCCGA AATATACGGC ATACGCCGTA CCGAAGTATC ACGTGGACGA CTCATCACCT 
AAAAGCGACA TAGACATCTA CAGAGAATTG GAGAAACTCG ATAAGATCTC TTCCGCACAG 
GTGTTACCAG ATCCGACTCT GGAGCTTCCA CAGAAGGAAT TGTCCTCGAT TCCGTACGTT 
CCAAATACGT CGCCCTTCAA GGATGTGTCA CCATTGAGGT ACACTTCCAA GCCGGCTCAA 
TACGACACTT CACCCCTGAA GACTTCTTAC GATCCCTACA AGACCTTTGA CTTCAAGCCC 
AAGTTGTCAC CTAAACAAGT TTCCACTAAT CCATTTGTGT CAGCTACGTA TGAGAAACCT 
GTGGTGGATA CCGCTTTCGA GTACATTAAC AATAAACCAG AGATCTCGAT CAATACGTAT 
GATCTACATT TGAAGAGTCC AATGATGACG AAGGAATCGC TAGAGTTTCG TGTCAGATAC 
GACGATGATC CAGTCAGAGA AGTTAACACC GATTACATGT CTCTCCAGCC AGAAAGTAGA 
TCGATTACTA CCCCGAGCAA GTCTCCTTAT TTCAGCAATG GAAATACCGA ACCGTTAACG 
CCTACGAAAT ATATTTCTAA GGAGGAAAGA ATCTTGGATA CTGGTGGTAC CTCGTTTCTG 
AACTATCCCA GCGAATCTTC CGACCTTCTT CGTCGAAAAT ACGACGCTTT GTCTCCTAAG 
GAGTACACTC ATACCAAGAG TACGACGGAT TATGATAGAC ACTTAGGCAC GTTACCTCCT 
ATAGAACCTG GAACAGAGAC AGGATCGTAT TTACCGACGA GACACCGAGA TTATCATTCT 
CTATCACCGA ATCGTCAGTT TCCTGATCAC TTTCCTTCTA CTATCAATCA TCACTACACC 
TCGAAACTTT CATCAGGTCT CTCTGACGAA TCGAAACGAC CGGTTTCTCA TCCGGAATTC 
CCTGGCAAGA TGAATGTTGG GAAATATGCA GAATTACCCG ATAGAAGTTC CAGCAGTTAC 
AAGAAATCAT CTCTTAGCTT AGGTAACATA GGACACTCGA GTAAAGGAGC TGATAATAAT 
TTCAACACTG TGACCAGTCC AAAAACGCAG TTCAGTCCCT TTCCCGTTAG AAACGCTCCT 
AGGAAGCCGA AAGAACTTAC GCTGAAATTG GGCCTCTATT CGCCCAAAGG TTCTGATACA 
GGACAGTTAA AAAGGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012243268.1
CDS	415..6375
Translation	MSGFFNSLKNFASGAAYAASSASKYEELEVEDTKMRFSLTPQPGESGFIQWLDAMKMVARLPGGIPPEFRKKLWLTLAERHLEQRGVDWKQAEKICFNEWSNPDDEELGIQIVKDLHRTGCSLFCGAAGRDNQAVLRRVLLGFARWNKSVGYCQGLNVLAALVLQVMDRAESAAVKVMIYLIEGVLPEGYFADNLRGLSVDMAVFRDLLRGRLPKLSKHLEALQNDAKDKATGSSYEPPLTNVFTMQWFLTLFCHCLPQEAVLRVWDLIFLEGDEILLRTALAIWEGLSDRIMTVTSADEFYSIMGVLTREMLEFTDTNNLIKNIVSMGSLHGVTGLREKHRYNITPWARKLSDDEDSDTEEDERLAVAAAMFSMAQRLKKDRIPQTIGALQAMAPSSDRERLALDITTLKQQYAKLRERQRQAHIILSAACARQTMVPPPTSQAMNHLLVGKSALVSGKNRPLSLPSGAATTKIRPTTLNQNRRDRQGVTLHWKDTKKPKQKPSNLPETVECSVVTMQSTEAELASPKRSNGDSDSDSTSTELCDEPDRLSDVDSEDLTSASESCVVSTDEEKSSRITGSPIREKPLDRSFLEEKMEPINAEDSKDSKSSEDNLGDISIAKITDQIRRLSAEDDNNSMVPQKFSVQSKESPEEQSTKESKKEKSISDLSIDYTSDSNIIKKSLLDLNEYDYLGFSGSKISSKEDEVLVHERHRSKQTTNEIVDIPRNEVATIKKDQTEDKITTTMKQDSLNIYLDYSASLDKKYDRIKDKSSLDVEIENEIRSDRYKTSFDFSSRISLDLKPYETSKVGSLTSDTADTVNTERTLYGKAYVGHLPISPVTVDQKLSRVTPSIHTTHNTTVLTPETPEIVHKTLENTSYNDRIQTTQTKIYSDLVKSPKTPESNKSFSSGETMGPDSKPSSLTVSPRTPVRSESRDFPMDKSLCSSVSSDSKTLVLHSADSDSTRDSTKTDAKKMYEISNSATPISMDSLQKSEASPKLVVSSSSESCSPSKLKSSERSFSSDLQSPISANSIKYTSNFSRRGQDDVSGSPMDLSSATSPFYPISNPNQKTPTSPYSASRRRSSLDSNETSPEQKSTSSRESNKFLGYQSKFDSSIKTSDMKDVDGIARKGDTFVRPEFITRNEDSSINLTDRRINVSDVKYYQTTSTDYYRKDKDIFDDGGDDPSSEKDVVPSLPQEKLDKHYLNYRYRDRSKLLERSSSSLDSRRYADMKSSASTDEFSTSIAQKRSSDILEDIKHLEAKANDGSSDNGMLTKKSSYIWEDMKNLEARRQDTFSDSARNFSKHRLEVPDINITGESRKSYVVHPKINIDENSDSILKTMACGKSNGTDDGRESKLGVWTKVKPRKKSDNGRRNSDRALKIIQENSAILQKILACQAKKRLPDLEEISKEITISPINEEISKIFSPILEKMGLNEHEINEELARINFKDFDNMTATSVSEFDAKINEELSKLSLIDETEQMDHFDVDEVISHEYLNTREALIDRKINEELSKLLANYDDHSPASMVSLDKGPSSQNISEIEGLDLSSISTNVFSYKSSNDSIDTRSDLGSTQEPSILVDKFPKYTAYAVPKYHVDDSSPKSDIDIYRELEKLDKISSAQVLPDPTLELPQKELSSIPYVPNTSPFKDVSPLRYTSKPAQYDTSPLKTSYDPYKTFDFKPKLSPKQVSTNPFVSATYEKPVVDTAFEYINNKPEISINTYDLHLKSPMMTKESLEFRVRYDDDPVREVNTDYMSLQPESRSITTPSKSPYFSNGNTEPLTPTKYISKEERILDTGGTSFLNYPSESSDLLRRKYDALSPKEYTHTKSTTDYDRHLGTLPPIEPGTETGSYLPTRHRDYHSLSPNRQFPDHFPSTINHHYTSKLSSGLSDESKRPVSHPEFPGKMNVGKYAELPDRSSSSYKKSSLSLGNIGHSSKGADNNFNTVTSPKTQFSPFPVRNAPRKPKELTLKLGLYSPKGSDTGQLKRS

Target ORF information:

RefSeq Version	XM_012387845.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012387845.1

ORF Insert Sequence:

ATGAGCGGAT TTTTCAACTC CCTGAAAAAC TTCGCGTCAG GAGCCGCGTA CGCTGCCAGC 
TCTGCCTCGA AGTACGAAGA ATTGGAAGTC GAAGACACGA AGATGAGATT TAGCCTGACG 
CCGCAACCAG GAGAAAGTGG TTTTATACAA TGGCTGGACG CGATGAAAAT GGTTGCCAGG 
TTACCAGGAG GAATTCCGCC GGAATTTCGA AAGAAGTTGT GGCTGACACT GGCAGAACGT 
CATCTAGAGC AGCGCGGCGT AGATTGGAAG CAGGCTGAAA AGATCTGCTT CAACGAGTGG 
AGCAATCCTG ACGATGAAGA GCTTGGTATA CAAATCGTTA AAGACCTACA CAGAACGGGC 
TGCAGTCTGT TTTGCGGTGC AGCTGGCAGA GACAATCAAG CAGTGCTTCG TCGAGTTCTG 
CTTGGTTTCG CCCGATGGAA CAAGTCCGTC GGCTATTGCC AGGGCCTGAA CGTGTTGGCC 
GCCCTCGTTT TGCAAGTAAT GGATCGCGCC GAGTCCGCCG CGGTGAAGGT GATGATCTAC 
TTGATAGAGG GTGTCCTGCC CGAGGGTTAC TTCGCGGACA ATCTCCGTGG CCTGTCGGTC 
GACATGGCGG TATTTCGAGA TCTCCTCCGG GGCAGACTGC CCAAGCTCTC GAAACATCTG 
GAGGCTCTGC AGAACGATGC TAAGGACAAG GCGACAGGCA GCAGTTACGA GCCACCTCTG 
ACGAATGTGT TCACGATGCA ATGGTTCCTT ACTCTTTTCT GTCACTGTTT GCCACAAGAG 
GCTGTCCTGC GCGTATGGGA CCTCATATTT CTCGAAGGAG ATGAGATATT GCTTAGAACC 
GCACTGGCCA TCTGGGAAGG ACTTTCCGAT CGTATTATGA CGGTGACATC GGCGGACGAG 
TTTTATAGTA TAATGGGAGT GCTGACAAGG GAGATGCTCG AGTTCACGGA TACTAATAAC 
CTCATAAAGA ATATCGTTAG TATGGGATCT TTGCATGGTG TAACGGGGCT AAGAGAGAAA 
CATAGATACA ATATCACACC TTGGGCAAGA AAATTGAGCG ACGACGAGGA CAGCGACACG 
GAGGAGGATG AAAGGCTGGC CGTGGCTGCT GCGATGTTCA GTATGGCACA GCGATTGAAG 
AAAGATCGTA TACCTCAAAC GATTGGAGCG CTGCAGGCAA TGGCACCCAG CAGCGACCGA 
GAACGGCTCG CTTTGGACAT TACCACTTTG AAACAGCAGT ACGCCAAGCT ACGCGAACGT 
CAACGACAAG CGCATATTAT ACTTTCTGCT GCATGTGCAA GGCAGACCAT GGTACCACCT 
CCTACTTCTC AGGCCATGAA TCATCTTTTA GTGGGCAAAA GTGCGCTCGT GAGTGGGAAG 
AATCGACCCC TGAGCCTACC TTCTGGTGCT GCAACGACGA AGATAAGACC TACGACGCTA 
AATCAAAACC GAAGGGACAG ACAAGGCGTC ACGCTTCACT GGAAAGACAC CAAAAAACCG 
AAGCAGAAAC CCTCGAATTT ACCAGAAACG GTGGAATGTA GCGTTGTAAC AATGCAATCC 
ACGGAAGCTG AGCTGGCTTC TCCAAAGCGG AGTAACGGTG ACAGTGATAG CGACAGCACA 
TCGACGGAAT TGTGCGACGA ACCGGACCGT CTCAGTGACG TTGACAGCGA AGATCTGACG 
TCAGCATCCG AATCCTGTGT TGTCAGTACG GATGAGGAGA AAAGCTCTCG AATCACCGGA 
TCACCGATTC GCGAGAAGCC ACTCGATCGT TCGTTTCTCG AAGAGAAGAT GGAACCAATT 
AACGCCGAAG ATTCGAAGGA CTCGAAAAGC TCCGAGGATA ACCTTGGTGA CATATCAATC 
GCTAAAATCA CCGACCAAAT AAGACGATTA TCAGCAGAGG ATGACAATAA TTCCATGGTT 
CCTCAAAAAT TTAGCGTGCA GAGCAAAGAA TCGCCGGAAG AACAATCGAC GAAAGAATCG 
AAAAAAGAGA AATCCATATC AGATCTATCT ATAGATTACA CTTCCGATAG TAATATCATT 
AAAAAATCGT TATTGGACTT GAACGAGTAC GATTATCTAG GTTTCAGTGG CAGTAAGATC 
AGTTCGAAAG AAGATGAAGT ATTAGTGCAC GAAAGGCATC GATCTAAACA GACAACAAAC 
GAGATTGTAG ATATACCTCG AAACGAAGTT GCGACAATCA AGAAAGACCA AACTGAAGAC 
AAGATAACTA CGACTATGAA ACAGGATTCT TTAAACATAT ACTTGGATTA TTCAGCATCC 
TTGGACAAGA AATACGATAG AATCAAAGAT AAATCAAGCC TCGATGTAGA GATAGAAAAT 
GAAATTAGAT CCGACCGATA TAAAACATCC TTCGATTTCT CTAGTAGAAT ATCTTTGGAC 
TTGAAACCGT ATGAAACTTC AAAAGTAGGA AGTTTAACCA GTGACACTGC AGACACAGTG 
AATACTGAAA GAACGCTTTA TGGCAAGGCC TACGTTGGTC ATCTTCCTAT TTCACCTGTA 
ACAGTTGATC AAAAATTAAG CAGAGTGACA CCCAGTATAC ATACAACGCA TAATACTACT 
GTATTAACTC CTGAAACGCC TGAAATTGTA CATAAAACGT TAGAGAACAC GTCATATAAC 
GATAGAATAC AGACGACTCA AACGAAAATT TATTCAGACC TAGTGAAGAG TCCTAAAACA 
CCGGAAAGCA ACAAATCATT CAGTTCAGGT GAAACCATGG GACCAGATTC GAAACCATCG 
AGTCTGACAG TTAGCCCAAG GACGCCGGTT AGATCAGAAT CTCGAGATTT CCCTATGGAC 
AAATCCCTGT GTTCGTCAGT TAGCTCAGAC TCGAAGACTT TGGTTCTTCA TTCTGCAGAT 
TCAGACAGTA CGAGGGACAG CACGAAAACC GACGCGAAGA AAATGTATGA GATTTCCAAT 
TCGGCGACAC CTATCAGCAT GGACTCGTTG CAGAAATCAG AAGCCAGCCC AAAACTAGTC 
GTGAGCAGCT CCAGTGAATC GTGTAGCCCG AGTAAATTAA AGTCTTCCGA GAGGTCGTTC 
AGTTCGGACC TGCAATCGCC AATAAGTGCC AACTCCATAA AGTATACTTC GAATTTCAGT 
AGACGGGGCC AAGATGACGT ATCAGGCTCG CCAATGGACT TGAGCAGCGC TACCTCGCCG 
TTTTATCCGA TCTCGAATCC GAATCAAAAA ACTCCGACGT CCCCGTACAG TGCTTCTAGA 
AGAAGATCCA GTTTGGACAG CAACGAAACG TCACCTGAGC AGAAATCGAC CAGTTCGAGG 
GAGTCGAACA AGTTTCTAGG TTATCAGTCG AAATTTGACT CCTCCATAAA GACGTCTGAT 
ATGAAAGATG TAGACGGTAT AGCACGAAAA GGTGACACAT TCGTCAGACC AGAGTTCATC 
ACGAGGAATG AAGATTCGAG TATTAATTTG ACGGATAGAA GAATCAATGT TAGTGATGTT 
AAGTATTATC AAACAACGAG TACGGATTAC TATCGCAAGG ATAAGGATAT TTTTGATGAC 
GGAGGGGATG ATCCGTCATC GGAGAAAGAC GTGGTACCTT CTTTGCCTCA GGAGAAGCTA 
GATAAACATT ATTTGAACTA TCGATATAGA GATAGATCGA AGTTGTTGGA AAGAAGTTCA 
AGCAGTTTGG ACAGTAGAAG GTACGCTGAT ATGAAGTCAT CGGCTTCCAC CGATGAATTT 
TCAACGTCGA TCGCGCAGAA GAGATCCAGT GACATTTTAG AAGATATTAA ACACTTGGAG 
GCGAAAGCAA ACGATGGGTC GTCGGATAAT GGGATGTTGA CGAAGAAGAG CAGTTATATT 
TGGGAGGATA TGAAGAATTT AGAAGCCAGG AGGCAAGATA CCTTTAGTGA TTCAGCGAGG 
AACTTTTCGA AACATCGTCT GGAGGTACCG GATATAAATA TAACCGGAGA AAGCAGAAAA 
TCGTACGTAG TACATCCGAA GATCAATATC GACGAAAATA GCGACAGCAT ATTGAAGACC 
ATGGCCTGTG GTAAGAGTAA CGGCACAGAT GATGGCAGGG AGTCGAAGTT GGGCGTGTGG 
ACGAAGGTGA AGCCTCGTAA AAAGAGTGAC AATGGGCGGA GGAACAGCGA CAGGGCGTTG 
AAGATCATTC AAGAAAACTC CGCTATACTT CAGAAGATTC TTGCGTGTCA GGCAAAAAAG 
CGTCTGCCAG ACCTGGAGGA GATATCGAAA GAAATTACCA TTAGCCCTAT AAACGAGGAG 
ATTTCGAAGA TCTTTAGTCC GATATTAGAG AAAATGGGAT TAAACGAACA CGAGATTAAC 
GAGGAATTGG CGAGAATCAA TTTCAAGGAT TTCGACAACA TGACCGCTAC CAGTGTGTCC 
GAGTTTGATG CAAAGATTAA CGAGGAATTA TCGAAGCTTT CTCTGATCGA CGAGACGGAG 
CAGATGGACC ACTTCGACGT GGACGAGGTG ATATCCCACG AATACTTGAA CACGAGAGAA 
GCTCTAATAG ATCGTAAGAT AAACGAGGAA TTGTCGAAAT TATTGGCAAA CTACGACGAT 
CATTCTCCGG CTAGTATGGT GAGTTTGGAC AAAGGACCAT CCAGCCAGAA TATTAGCGAG 
ATCGAAGGAC TGGATCTGTC CAGTATTTCA ACTAACGTAT TTTCCTATAA ATCATCCAAT 
GACTCCATTG ACACGAGAAG CGACTTAGGC TCGACGCAGG AACCATCCAT TCTCGTTGAC 
AAGTTTCCGA AATATACGGC ATACGCCGTA CCGAAGTATC ACGTGGACGA CTCATCACCT 
AAAAGCGACA TAGACATCTA CAGAGAATTG GAGAAACTCG ATAAGATCTC TTCCGCACAG 
GTGTTACCAG ATCCGACTCT GGAGCTTCCA CAGAAGGAAT TGTCCTCGAT TCCGTACGTT 
CCAAATACGT CGCCCTTCAA GGATGTGTCA CCATTGAGGT ACACTTCCAA GCCGGCTCAA 
TACGACACTT CACCCCTGAA GACTTCTTAC GATCCCTACA AGACCTTTGA CTTCAAGCCC 
AAGTTGTCAC CTAAACAAGT TTCCACTAAT CCATTTGTGT CAGCTACGTA TGAGAAACCT 
GTGGTGGATA CCGCTTTCGA GTACATTAAC AATAAACCAG AGATCTCGAT CAATACGTAT 
GATCTACATT TGAAGAGTCC AATGATGACG AAGGAATCGC TAGAGTTTCG TGTCAGATAC 
GACGATGATC CAGTCAGAGA AGTTAACACC GATTACATGT CTCTCCAGCC AGAAAGTAGA 
TCGATTACTA CCCCGAGCAA GTCTCCTTAT TTCAGCAATG GAAATACCGA ACCGTTAACG 
CCTACGAAAT ATATTTCTAA GGAGGAAAGA ATCTTGGATA CTGGTGGTAC CTCGTTTCTG 
AACTATCCCA GCGAATCTTC CGACCTTCTT CGTCGAAAAT ACGACGCTTT GTCTCCTAAG 
GAGTACACTC ATACCAAGAG TACGACGGAT TATGATAGAC ACTTAGGCAC GTTACCTCCT 
ATAGAACCTG GAACAGAGAC AGGATCGTAT TTACCGACGA GACACCGAGA TTATCATTCT 
CTATCACCGA ATCGTCAGTT TCCTGATCAC TTTCCTTCTA CTATCAATCA TCACTACACC 
TCGAAACTTT CATCAGGTCT CTCTGACGAA TCGAAACGAC CGGTTTCTCA TCCGGAATTC 
CCTGGCAAGA TGAATGTTGG GAAATATGCA GAATTACCCG ATAGAAGTTC CAGCAGTTAC 
AAGAAATCAT CTCTTAGCTT AGGTAACATA GGACACTCGA GTAAAGGAGC TGATAATAAT 
TTCAACACTG TGACCAGTCC AAAAACGCAG TTCAGTCCCT TTCCCGTTAG AAACGCTCCT 
AGGAAGCCGA AAGAACTTAC GCTGAAATTG GGCCTCTATT CGCCCAAAGG TTCTGATACA 
GGACAGTTAA AAAGGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100749006 ( XM_012387846.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012243269.1 Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X3, mRNA.

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CloneID	OBo106113
Clone ID Related Accession (Same CDS sequence)	XM_012387845.2 , XM_024368866.1 , XM_012387845.1 , XM_012387846.1
Accession Version	XM_012387846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	uncharacterized protein LOC100749006 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176906.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGAT TTTTCAACTC CCTGAAAAAC TTCGCGTCAG GAGCCGCGTA CGCTGCCAGC 
TCTGCCTCGA AGTACGAAGA ATTGGAAGTC GAAGACACGA AGATGAGATT TAGCCTGACG 
CCGCAACCAG GAGAAAGTGG TTTTATACAA TGGCTGGACG CGATGAAAAT GGTTGCCAGG 
TTACCAGGAG GAATTCCGCC GGAATTTCGA AAGAAGTTGT GGCTGACACT GGCAGAACGT 
CATCTAGAGC AGCGCGGCGT AGATTGGAAG CAGGCTGAAA AGATCTGCTT CAACGAGTGG 
AGCAATCCTG ACGATGAAGA GCTTGGTATA CAAATCGTTA AAGACCTACA CAGAACGGGC 
TGCAGTCTGT TTTGCGGTGC AGCTGGCAGA GACAATCAAG CAGTGCTTCG TCGAGTTCTG 
CTTGGTTTCG CCCGATGGAA CAAGTCCGTC GGCTATTGCC AGGGCCTGAA CGTGTTGGCC 
GCCCTCGTTT TGCAAGTAAT GGATCGCGCC GAGTCCGCCG CGGTGAAGGT GATGATCTAC 
TTGATAGAGG GTGTCCTGCC CGAGGGTTAC TTCGCGGACA ATCTCCGTGG CCTGTCGGTC 
GACATGGCGG TATTTCGAGA TCTCCTCCGG GGCAGACTGC CCAAGCTCTC GAAACATCTG 
GAGGCTCTGC AGAACGATGC TAAGGACAAG GCGACAGGCA GCAGTTACGA GCCACCTCTG 
ACGAATGTGT TCACGATGCA ATGGTTCCTT ACTCTTTTCT GTCACTGTTT GCCACAAGAG 
GCTGTCCTGC GCGTATGGGA CCTCATATTT CTCGAAGGAG ATGAGATATT GCTTAGAACC 
GCACTGGCCA TCTGGGAAGG ACTTTCCGAT CGTATTATGA CGGTGACATC GGCGGACGAG 
TTTTATAGTA TAATGGGAGT GCTGACAAGG GAGATGCTCG AGTTCACGGA TACTAATAAC 
CTCATAAAGA ATATCGTTAG TATGGGATCT TTGCATGGTG TAACGGGGCT AAGAGAGAAA 
CATAGATACA ATATCACACC TTGGGCAAGA AAATTGAGCG ACGACGAGGA CAGCGACACG 
GAGGAGGATG AAAGGCTGGC CGTGGCTGCT GCGATGTTCA GTATGGCACA GCGATTGAAG 
AAAGATCGTA TACCTCAAAC GATTGGAGCG CTGCAGGCAA TGGCACCCAG CAGCGACCGA 
GAACGGCTCG CTTTGGACAT TACCACTTTG AAACAGCAGT ACGCCAAGCT ACGCGAACGT 
CAACGACAAG CGCATATTAT ACTTTCTGCT GCATGTGCAA GGCAGACCAT GGTACCACCT 
CCTACTTCTC AGGCCATGAA TCATCTTTTA GTGGGCAAAA GTGCGCTCGT GAGTGGGAAG 
AATCGACCCC TGAGCCTACC TTCTGGTGCT GCAACGACGA AGATAAGACC TACGACGCTA 
AATCAAAACC GAAGGGACAG ACAAGGCGTC ACGCTTCACT GGAAAGACAC CAAAAAACCG 
AAGCAGAAAC CCTCGAATTT ACCAGAAACG GTGGAATGTA GCGTTGTAAC AATGCAATCC 
ACGGAAGCTG AGCTGGCTTC TCCAAAGCGG AGTAACGGTG ACAGTGATAG CGACAGCACA 
TCGACGGAAT TGTGCGACGA ACCGGACCGT CTCAGTGACG TTGACAGCGA AGATCTGACG 
TCAGCATCCG AATCCTGTGT TGTCAGTACG GATGAGGAGA AAAGCTCTCG AATCACCGGA 
TCACCGATTC GCGAGAAGCC ACTCGATCGT TCGTTTCTCG AAGAGAAGAT GGAACCAATT 
AACGCCGAAG ATTCGAAGGA CTCGAAAAGC TCCGAGGATA ACCTTGGTGA CATATCAATC 
GCTAAAATCA CCGACCAAAT AAGACGATTA TCAGCAGAGG ATGACAATAA TTCCATGGTT 
CCTCAAAAAT TTAGCGTGCA GAGCAAAGAA TCGCCGGAAG AACAATCGAC GAAAGAATCG 
AAAAAAGAGA AATCCATATC AGATCTATCT ATAGATTACA CTTCCGATAG TAATATCATT 
AAAAAATCGT TATTGGACTT GAACGAGTAC GATTATCTAG GTTTCAGTGG CAGTAAGATC 
AGTTCGAAAG AAGATGAAGT ATTAGTGCAC GAAAGGCATC GATCTAAACA GACAACAAAC 
GAGATTGTAG ATATACCTCG AAACGAAGTT GCGACAATCA AGAAAGACCA AACTGAAGAC 
AAGATAACTA CGACTATGAA ACAGGATTCT TTAAACATAT ACTTGGATTA TTCAGCATCC 
TTGGACAAGA AATACGATAG AATCAAAGAT AAATCAAGCC TCGATGTAGA GATAGAAAAT 
GAAATTAGAT CCGACCGATA TAAAACATCC TTCGATTTCT CTAGTAGAAT ATCTTTGGAC 
TTGAAACCGT ATGAAACTTC AAAAGTAGGA AGTTTAACCA GTGACACTGC AGACACAGTG 
AATACTGAAA GAACGCTTTA TGGCAAGGCC TACGTTGGTC ATCTTCCTAT TTCACCTGTA 
ACAGTTGATC AAAAATTAAG CAGAGTGACA CCCAGTATAC ATACAACGCA TAATACTACT 
GTATTAACTC CTGAAACGCC TGAAATTGTA CATAAAACGT TAGAGAACAC GTCATATAAC 
GATAGAATAC AGACGACTCA AACGAAAATT TATTCAGACC TAGTGAAGAG TCCTAAAACA 
CCGGAAAGCA ACAAATCATT CAGTTCAGGT GAAACCATGG GACCAGATTC GAAACCATCG 
AGTCTGACAG TTAGCCCAAG GACGCCGGTT AGATCAGAAT CTCGAGATTT CCCTATGGAC 
AAATCCCTGT GTTCGTCAGT TAGCTCAGAC TCGAAGACTT TGGTTCTTCA TTCTGCAGAT 
TCAGACAGTA CGAGGGACAG CACGAAAACC GACGCGAAGA AAATGTATGA GATTTCCAAT 
TCGGCGACAC CTATCAGCAT GGACTCGTTG CAGAAATCAG AAGCCAGCCC AAAACTAGTC 
GTGAGCAGCT CCAGTGAATC GTGTAGCCCG AGTAAATTAA AGTCTTCCGA GAGGTCGTTC 
AGTTCGGACC TGCAATCGCC AATAAGTGCC AACTCCATAA AGTATACTTC GAATTTCAGT 
AGACGGGGCC AAGATGACGT ATCAGGCTCG CCAATGGACT TGAGCAGCGC TACCTCGCCG 
TTTTATCCGA TCTCGAATCC GAATCAAAAA ACTCCGACGT CCCCGTACAG TGCTTCTAGA 
AGAAGATCCA GTTTGGACAG CAACGAAACG TCACCTGAGC AGAAATCGAC CAGTTCGAGG 
GAGTCGAACA AGTTTCTAGG TTATCAGTCG AAATTTGACT CCTCCATAAA GACGTCTGAT 
ATGAAAGATG TAGACGGTAT AGCACGAAAA GGTGACACAT TCGTCAGACC AGAGTTCATC 
ACGAGGAATG AAGATTCGAG TATTAATTTG ACGGATAGAA GAATCAATGT TAGTGATGTT 
AAGTATTATC AAACAACGAG TACGGATTAC TATCGCAAGG ATAAGGATAT TTTTGATGAC 
GGAGGGGATG ATCCGTCATC GGAGAAAGAC GTGGTACCTT CTTTGCCTCA GGAGAAGCTA 
GATAAACATT ATTTGAACTA TCGATATAGA GATAGATCGA AGTTGTTGGA AAGAAGTTCA 
AGCAGTTTGG ACAGTAGAAG GTACGCTGAT ATGAAGTCAT CGGCTTCCAC CGATGAATTT 
TCAACGTCGA TCGCGCAGAA GAGATCCAGT GACATTTTAG AAGATATTAA ACACTTGGAG 
GCGAAAGCAA ACGATGGGTC GTCGGATAAT GGGATGTTGA CGAAGAAGAG CAGTTATATT 
TGGGAGGATA TGAAGAATTT AGAAGCCAGG AGGCAAGATA CCTTTAGTGA TTCAGCGAGG 
AACTTTTCGA AACATCGTCT GGAGGTACCG GATATAAATA TAACCGGAGA AAGCAGAAAA 
TCGTACGTAG TACATCCGAA GATCAATATC GACGAAAATA GCGACAGCAT ATTGAAGACC 
ATGGCCTGTG GTAAGAGTAA CGGCACAGAT GATGGCAGGG AGTCGAAGTT GGGCGTGTGG 
ACGAAGGTGA AGCCTCGTAA AAAGAGTGAC AATGGGCGGA GGAACAGCGA CAGGGCGTTG 
AAGATCATTC AAGAAAACTC CGCTATACTT CAGAAGATTC TTGCGTGTCA GGCAAAAAAG 
CGTCTGCCAG ACCTGGAGGA GATATCGAAA GAAATTACCA TTAGCCCTAT AAACGAGGAG 
ATTTCGAAGA TCTTTAGTCC GATATTAGAG AAAATGGGAT TAAACGAACA CGAGATTAAC 
GAGGAATTGG CGAGAATCAA TTTCAAGGAT TTCGACAACA TGACCGCTAC CAGTGTGTCC 
GAGTTTGATG CAAAGATTAA CGAGGAATTA TCGAAGCTTT CTCTGATCGA CGAGACGGAG 
CAGATGGACC ACTTCGACGT GGACGAGGTG ATATCCCACG AATACTTGAA CACGAGAGAA 
GCTCTAATAG ATCGTAAGAT AAACGAGGAA TTGTCGAAAT TATTGGCAAA CTACGACGAT 
CATTCTCCGG CTAGTATGGT GAGTTTGGAC AAAGGACCAT CCAGCCAGAA TATTAGCGAG 
ATCGAAGGAC TGGATCTGTC CAGTATTTCA ACTAACGTAT TTTCCTATAA ATCATCCAAT 
GACTCCATTG ACACGAGAAG CGACTTAGGC TCGACGCAGG AACCATCCAT TCTCGTTGAC 
AAGTTTCCGA AATATACGGC ATACGCCGTA CCGAAGTATC ACGTGGACGA CTCATCACCT 
AAAAGCGACA TAGACATCTA CAGAGAATTG GAGAAACTCG ATAAGATCTC TTCCGCACAG 
GTGTTACCAG ATCCGACTCT GGAGCTTCCA CAGAAGGAAT TGTCCTCGAT TCCGTACGTT 
CCAAATACGT CGCCCTTCAA GGATGTGTCA CCATTGAGGT ACACTTCCAA GCCGGCTCAA 
TACGACACTT CACCCCTGAA GACTTCTTAC GATCCCTACA AGACCTTTGA CTTCAAGCCC 
AAGTTGTCAC CTAAACAAGT TTCCACTAAT CCATTTGTGT CAGCTACGTA TGAGAAACCT 
GTGGTGGATA CCGCTTTCGA GTACATTAAC AATAAACCAG AGATCTCGAT CAATACGTAT 
GATCTACATT TGAAGAGTCC AATGATGACG AAGGAATCGC TAGAGTTTCG TGTCAGATAC 
GACGATGATC CAGTCAGAGA AGTTAACACC GATTACATGT CTCTCCAGCC AGAAAGTAGA 
TCGATTACTA CCCCGAGCAA GTCTCCTTAT TTCAGCAATG GAAATACCGA ACCGTTAACG 
CCTACGAAAT ATATTTCTAA GGAGGAAAGA ATCTTGGATA CTGGTGGTAC CTCGTTTCTG 
AACTATCCCA GCGAATCTTC CGACCTTCTT CGTCGAAAAT ACGACGCTTT GTCTCCTAAG 
GAGTACACTC ATACCAAGAG TACGACGGAT TATGATAGAC ACTTAGGCAC GTTACCTCCT 
ATAGAACCTG GAACAGAGAC AGGATCGTAT TTACCGACGA GACACCGAGA TTATCATTCT 
CTATCACCGA ATCGTCAGTT TCCTGATCAC TTTCCTTCTA CTATCAATCA TCACTACACC 
TCGAAACTTT CATCAGGTCT CTCTGACGAA TCGAAACGAC CGGTTTCTCA TCCGGAATTC 
CCTGGCAAGA TGAATGTTGG GAAATATGCA GAATTACCCG ATAGAAGTTC CAGCAGTTAC 
AAGAAATCAT CTCTTAGCTT AGGTAACATA GGACACTCGA GTAAAGGAGC TGATAATAAT 
TTCAACACTG TGACCAGTCC AAAAACGCAG TTCAGTCCCT TTCCCGTTAG AAACGCTCCT 
AGGAAGCCGA AAGAACTTAC GCTGAAATTG GGCCTCTATT CGCCCAAAGG TTCTGATACA 
GGACAGTTAA AAAGGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012243269.1
CDS	212..6172
Translation	MSGFFNSLKNFASGAAYAASSASKYEELEVEDTKMRFSLTPQPGESGFIQWLDAMKMVARLPGGIPPEFRKKLWLTLAERHLEQRGVDWKQAEKICFNEWSNPDDEELGIQIVKDLHRTGCSLFCGAAGRDNQAVLRRVLLGFARWNKSVGYCQGLNVLAALVLQVMDRAESAAVKVMIYLIEGVLPEGYFADNLRGLSVDMAVFRDLLRGRLPKLSKHLEALQNDAKDKATGSSYEPPLTNVFTMQWFLTLFCHCLPQEAVLRVWDLIFLEGDEILLRTALAIWEGLSDRIMTVTSADEFYSIMGVLTREMLEFTDTNNLIKNIVSMGSLHGVTGLREKHRYNITPWARKLSDDEDSDTEEDERLAVAAAMFSMAQRLKKDRIPQTIGALQAMAPSSDRERLALDITTLKQQYAKLRERQRQAHIILSAACARQTMVPPPTSQAMNHLLVGKSALVSGKNRPLSLPSGAATTKIRPTTLNQNRRDRQGVTLHWKDTKKPKQKPSNLPETVECSVVTMQSTEAELASPKRSNGDSDSDSTSTELCDEPDRLSDVDSEDLTSASESCVVSTDEEKSSRITGSPIREKPLDRSFLEEKMEPINAEDSKDSKSSEDNLGDISIAKITDQIRRLSAEDDNNSMVPQKFSVQSKESPEEQSTKESKKEKSISDLSIDYTSDSNIIKKSLLDLNEYDYLGFSGSKISSKEDEVLVHERHRSKQTTNEIVDIPRNEVATIKKDQTEDKITTTMKQDSLNIYLDYSASLDKKYDRIKDKSSLDVEIENEIRSDRYKTSFDFSSRISLDLKPYETSKVGSLTSDTADTVNTERTLYGKAYVGHLPISPVTVDQKLSRVTPSIHTTHNTTVLTPETPEIVHKTLENTSYNDRIQTTQTKIYSDLVKSPKTPESNKSFSSGETMGPDSKPSSLTVSPRTPVRSESRDFPMDKSLCSSVSSDSKTLVLHSADSDSTRDSTKTDAKKMYEISNSATPISMDSLQKSEASPKLVVSSSSESCSPSKLKSSERSFSSDLQSPISANSIKYTSNFSRRGQDDVSGSPMDLSSATSPFYPISNPNQKTPTSPYSASRRRSSLDSNETSPEQKSTSSRESNKFLGYQSKFDSSIKTSDMKDVDGIARKGDTFVRPEFITRNEDSSINLTDRRINVSDVKYYQTTSTDYYRKDKDIFDDGGDDPSSEKDVVPSLPQEKLDKHYLNYRYRDRSKLLERSSSSLDSRRYADMKSSASTDEFSTSIAQKRSSDILEDIKHLEAKANDGSSDNGMLTKKSSYIWEDMKNLEARRQDTFSDSARNFSKHRLEVPDINITGESRKSYVVHPKINIDENSDSILKTMACGKSNGTDDGRESKLGVWTKVKPRKKSDNGRRNSDRALKIIQENSAILQKILACQAKKRLPDLEEISKEITISPINEEISKIFSPILEKMGLNEHEINEELARINFKDFDNMTATSVSEFDAKINEELSKLSLIDETEQMDHFDVDEVISHEYLNTREALIDRKINEELSKLLANYDDHSPASMVSLDKGPSSQNISEIEGLDLSSISTNVFSYKSSNDSIDTRSDLGSTQEPSILVDKFPKYTAYAVPKYHVDDSSPKSDIDIYRELEKLDKISSAQVLPDPTLELPQKELSSIPYVPNTSPFKDVSPLRYTSKPAQYDTSPLKTSYDPYKTFDFKPKLSPKQVSTNPFVSATYEKPVVDTAFEYINNKPEISINTYDLHLKSPMMTKESLEFRVRYDDDPVREVNTDYMSLQPESRSITTPSKSPYFSNGNTEPLTPTKYISKEERILDTGGTSFLNYPSESSDLLRRKYDALSPKEYTHTKSTTDYDRHLGTLPPIEPGTETGSYLPTRHRDYHSLSPNRQFPDHFPSTINHHYTSKLSSGLSDESKRPVSHPEFPGKMNVGKYAELPDRSSSSYKKSSLSLGNIGHSSKGADNNFNTVTSPKTQFSPFPVRNAPRKPKELTLKLGLYSPKGSDTGQLKRS

Target ORF information:

RefSeq Version	XM_012387846.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012387846.1

ORF Insert Sequence:

ATGAGCGGAT TTTTCAACTC CCTGAAAAAC TTCGCGTCAG GAGCCGCGTA CGCTGCCAGC 
TCTGCCTCGA AGTACGAAGA ATTGGAAGTC GAAGACACGA AGATGAGATT TAGCCTGACG 
CCGCAACCAG GAGAAAGTGG TTTTATACAA TGGCTGGACG CGATGAAAAT GGTTGCCAGG 
TTACCAGGAG GAATTCCGCC GGAATTTCGA AAGAAGTTGT GGCTGACACT GGCAGAACGT 
CATCTAGAGC AGCGCGGCGT AGATTGGAAG CAGGCTGAAA AGATCTGCTT CAACGAGTGG 
AGCAATCCTG ACGATGAAGA GCTTGGTATA CAAATCGTTA AAGACCTACA CAGAACGGGC 
TGCAGTCTGT TTTGCGGTGC AGCTGGCAGA GACAATCAAG CAGTGCTTCG TCGAGTTCTG 
CTTGGTTTCG CCCGATGGAA CAAGTCCGTC GGCTATTGCC AGGGCCTGAA CGTGTTGGCC 
GCCCTCGTTT TGCAAGTAAT GGATCGCGCC GAGTCCGCCG CGGTGAAGGT GATGATCTAC 
TTGATAGAGG GTGTCCTGCC CGAGGGTTAC TTCGCGGACA ATCTCCGTGG CCTGTCGGTC 
GACATGGCGG TATTTCGAGA TCTCCTCCGG GGCAGACTGC CCAAGCTCTC GAAACATCTG 
GAGGCTCTGC AGAACGATGC TAAGGACAAG GCGACAGGCA GCAGTTACGA GCCACCTCTG 
ACGAATGTGT TCACGATGCA ATGGTTCCTT ACTCTTTTCT GTCACTGTTT GCCACAAGAG 
GCTGTCCTGC GCGTATGGGA CCTCATATTT CTCGAAGGAG ATGAGATATT GCTTAGAACC 
GCACTGGCCA TCTGGGAAGG ACTTTCCGAT CGTATTATGA CGGTGACATC GGCGGACGAG 
TTTTATAGTA TAATGGGAGT GCTGACAAGG GAGATGCTCG AGTTCACGGA TACTAATAAC 
CTCATAAAGA ATATCGTTAG TATGGGATCT TTGCATGGTG TAACGGGGCT AAGAGAGAAA 
CATAGATACA ATATCACACC TTGGGCAAGA AAATTGAGCG ACGACGAGGA CAGCGACACG 
GAGGAGGATG AAAGGCTGGC CGTGGCTGCT GCGATGTTCA GTATGGCACA GCGATTGAAG 
AAAGATCGTA TACCTCAAAC GATTGGAGCG CTGCAGGCAA TGGCACCCAG CAGCGACCGA 
GAACGGCTCG CTTTGGACAT TACCACTTTG AAACAGCAGT ACGCCAAGCT ACGCGAACGT 
CAACGACAAG CGCATATTAT ACTTTCTGCT GCATGTGCAA GGCAGACCAT GGTACCACCT 
CCTACTTCTC AGGCCATGAA TCATCTTTTA GTGGGCAAAA GTGCGCTCGT GAGTGGGAAG 
AATCGACCCC TGAGCCTACC TTCTGGTGCT GCAACGACGA AGATAAGACC TACGACGCTA 
AATCAAAACC GAAGGGACAG ACAAGGCGTC ACGCTTCACT GGAAAGACAC CAAAAAACCG 
AAGCAGAAAC CCTCGAATTT ACCAGAAACG GTGGAATGTA GCGTTGTAAC AATGCAATCC 
ACGGAAGCTG AGCTGGCTTC TCCAAAGCGG AGTAACGGTG ACAGTGATAG CGACAGCACA 
TCGACGGAAT TGTGCGACGA ACCGGACCGT CTCAGTGACG TTGACAGCGA AGATCTGACG 
TCAGCATCCG AATCCTGTGT TGTCAGTACG GATGAGGAGA AAAGCTCTCG AATCACCGGA 
TCACCGATTC GCGAGAAGCC ACTCGATCGT TCGTTTCTCG AAGAGAAGAT GGAACCAATT 
AACGCCGAAG ATTCGAAGGA CTCGAAAAGC TCCGAGGATA ACCTTGGTGA CATATCAATC 
GCTAAAATCA CCGACCAAAT AAGACGATTA TCAGCAGAGG ATGACAATAA TTCCATGGTT 
CCTCAAAAAT TTAGCGTGCA GAGCAAAGAA TCGCCGGAAG AACAATCGAC GAAAGAATCG 
AAAAAAGAGA AATCCATATC AGATCTATCT ATAGATTACA CTTCCGATAG TAATATCATT 
AAAAAATCGT TATTGGACTT GAACGAGTAC GATTATCTAG GTTTCAGTGG CAGTAAGATC 
AGTTCGAAAG AAGATGAAGT ATTAGTGCAC GAAAGGCATC GATCTAAACA GACAACAAAC 
GAGATTGTAG ATATACCTCG AAACGAAGTT GCGACAATCA AGAAAGACCA AACTGAAGAC 
AAGATAACTA CGACTATGAA ACAGGATTCT TTAAACATAT ACTTGGATTA TTCAGCATCC 
TTGGACAAGA AATACGATAG AATCAAAGAT AAATCAAGCC TCGATGTAGA GATAGAAAAT 
GAAATTAGAT CCGACCGATA TAAAACATCC TTCGATTTCT CTAGTAGAAT ATCTTTGGAC 
TTGAAACCGT ATGAAACTTC AAAAGTAGGA AGTTTAACCA GTGACACTGC AGACACAGTG 
AATACTGAAA GAACGCTTTA TGGCAAGGCC TACGTTGGTC ATCTTCCTAT TTCACCTGTA 
ACAGTTGATC AAAAATTAAG CAGAGTGACA CCCAGTATAC ATACAACGCA TAATACTACT 
GTATTAACTC CTGAAACGCC TGAAATTGTA CATAAAACGT TAGAGAACAC GTCATATAAC 
GATAGAATAC AGACGACTCA AACGAAAATT TATTCAGACC TAGTGAAGAG TCCTAAAACA 
CCGGAAAGCA ACAAATCATT CAGTTCAGGT GAAACCATGG GACCAGATTC GAAACCATCG 
AGTCTGACAG TTAGCCCAAG GACGCCGGTT AGATCAGAAT CTCGAGATTT CCCTATGGAC 
AAATCCCTGT GTTCGTCAGT TAGCTCAGAC TCGAAGACTT TGGTTCTTCA TTCTGCAGAT 
TCAGACAGTA CGAGGGACAG CACGAAAACC GACGCGAAGA AAATGTATGA GATTTCCAAT 
TCGGCGACAC CTATCAGCAT GGACTCGTTG CAGAAATCAG AAGCCAGCCC AAAACTAGTC 
GTGAGCAGCT CCAGTGAATC GTGTAGCCCG AGTAAATTAA AGTCTTCCGA GAGGTCGTTC 
AGTTCGGACC TGCAATCGCC AATAAGTGCC AACTCCATAA AGTATACTTC GAATTTCAGT 
AGACGGGGCC AAGATGACGT ATCAGGCTCG CCAATGGACT TGAGCAGCGC TACCTCGCCG 
TTTTATCCGA TCTCGAATCC GAATCAAAAA ACTCCGACGT CCCCGTACAG TGCTTCTAGA 
AGAAGATCCA GTTTGGACAG CAACGAAACG TCACCTGAGC AGAAATCGAC CAGTTCGAGG 
GAGTCGAACA AGTTTCTAGG TTATCAGTCG AAATTTGACT CCTCCATAAA GACGTCTGAT 
ATGAAAGATG TAGACGGTAT AGCACGAAAA GGTGACACAT TCGTCAGACC AGAGTTCATC 
ACGAGGAATG AAGATTCGAG TATTAATTTG ACGGATAGAA GAATCAATGT TAGTGATGTT 
AAGTATTATC AAACAACGAG TACGGATTAC TATCGCAAGG ATAAGGATAT TTTTGATGAC 
GGAGGGGATG ATCCGTCATC GGAGAAAGAC GTGGTACCTT CTTTGCCTCA GGAGAAGCTA 
GATAAACATT ATTTGAACTA TCGATATAGA GATAGATCGA AGTTGTTGGA AAGAAGTTCA 
AGCAGTTTGG ACAGTAGAAG GTACGCTGAT ATGAAGTCAT CGGCTTCCAC CGATGAATTT 
TCAACGTCGA TCGCGCAGAA GAGATCCAGT GACATTTTAG AAGATATTAA ACACTTGGAG 
GCGAAAGCAA ACGATGGGTC GTCGGATAAT GGGATGTTGA CGAAGAAGAG CAGTTATATT 
TGGGAGGATA TGAAGAATTT AGAAGCCAGG AGGCAAGATA CCTTTAGTGA TTCAGCGAGG 
AACTTTTCGA AACATCGTCT GGAGGTACCG GATATAAATA TAACCGGAGA AAGCAGAAAA 
TCGTACGTAG TACATCCGAA GATCAATATC GACGAAAATA GCGACAGCAT ATTGAAGACC 
ATGGCCTGTG GTAAGAGTAA CGGCACAGAT GATGGCAGGG AGTCGAAGTT GGGCGTGTGG 
ACGAAGGTGA AGCCTCGTAA AAAGAGTGAC AATGGGCGGA GGAACAGCGA CAGGGCGTTG 
AAGATCATTC AAGAAAACTC CGCTATACTT CAGAAGATTC TTGCGTGTCA GGCAAAAAAG 
CGTCTGCCAG ACCTGGAGGA GATATCGAAA GAAATTACCA TTAGCCCTAT AAACGAGGAG 
ATTTCGAAGA TCTTTAGTCC GATATTAGAG AAAATGGGAT TAAACGAACA CGAGATTAAC 
GAGGAATTGG CGAGAATCAA TTTCAAGGAT TTCGACAACA TGACCGCTAC CAGTGTGTCC 
GAGTTTGATG CAAAGATTAA CGAGGAATTA TCGAAGCTTT CTCTGATCGA CGAGACGGAG 
CAGATGGACC ACTTCGACGT GGACGAGGTG ATATCCCACG AATACTTGAA CACGAGAGAA 
GCTCTAATAG ATCGTAAGAT AAACGAGGAA TTGTCGAAAT TATTGGCAAA CTACGACGAT 
CATTCTCCGG CTAGTATGGT GAGTTTGGAC AAAGGACCAT CCAGCCAGAA TATTAGCGAG 
ATCGAAGGAC TGGATCTGTC CAGTATTTCA ACTAACGTAT TTTCCTATAA ATCATCCAAT 
GACTCCATTG ACACGAGAAG CGACTTAGGC TCGACGCAGG AACCATCCAT TCTCGTTGAC 
AAGTTTCCGA AATATACGGC ATACGCCGTA CCGAAGTATC ACGTGGACGA CTCATCACCT 
AAAAGCGACA TAGACATCTA CAGAGAATTG GAGAAACTCG ATAAGATCTC TTCCGCACAG 
GTGTTACCAG ATCCGACTCT GGAGCTTCCA CAGAAGGAAT TGTCCTCGAT TCCGTACGTT 
CCAAATACGT CGCCCTTCAA GGATGTGTCA CCATTGAGGT ACACTTCCAA GCCGGCTCAA 
TACGACACTT CACCCCTGAA GACTTCTTAC GATCCCTACA AGACCTTTGA CTTCAAGCCC 
AAGTTGTCAC CTAAACAAGT TTCCACTAAT CCATTTGTGT CAGCTACGTA TGAGAAACCT 
GTGGTGGATA CCGCTTTCGA GTACATTAAC AATAAACCAG AGATCTCGAT CAATACGTAT 
GATCTACATT TGAAGAGTCC AATGATGACG AAGGAATCGC TAGAGTTTCG TGTCAGATAC 
GACGATGATC CAGTCAGAGA AGTTAACACC GATTACATGT CTCTCCAGCC AGAAAGTAGA 
TCGATTACTA CCCCGAGCAA GTCTCCTTAT TTCAGCAATG GAAATACCGA ACCGTTAACG 
CCTACGAAAT ATATTTCTAA GGAGGAAAGA ATCTTGGATA CTGGTGGTAC CTCGTTTCTG 
AACTATCCCA GCGAATCTTC CGACCTTCTT CGTCGAAAAT ACGACGCTTT GTCTCCTAAG 
GAGTACACTC ATACCAAGAG TACGACGGAT TATGATAGAC ACTTAGGCAC GTTACCTCCT 
ATAGAACCTG GAACAGAGAC AGGATCGTAT TTACCGACGA GACACCGAGA TTATCATTCT 
CTATCACCGA ATCGTCAGTT TCCTGATCAC TTTCCTTCTA CTATCAATCA TCACTACACC 
TCGAAACTTT CATCAGGTCT CTCTGACGAA TCGAAACGAC CGGTTTCTCA TCCGGAATTC 
CCTGGCAAGA TGAATGTTGG GAAATATGCA GAATTACCCG ATAGAAGTTC CAGCAGTTAC 
AAGAAATCAT CTCTTAGCTT AGGTAACATA GGACACTCGA GTAAAGGAGC TGATAATAAT 
TTCAACACTG TGACCAGTCC AAAAACGCAG TTCAGTCCCT TTCCCGTTAG AAACGCTCCT 
AGGAAGCCGA AAGAACTTAC GCTGAAATTG GGCCTCTATT CGCCCAAAGG TTCTGATACA 
GGACAGTTAA AAAGGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100749006 ( XM_012387847.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012243270.1 Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X4, mRNA.

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CloneID	OBo106114
Clone ID Related Accession (Same CDS sequence)	XM_012387847.1
Accession Version	XM_012387847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	uncharacterized protein LOC100749006 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176906.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTA GCCTGACGCC GCAACCAGGA GAAAGTGGTT TTATACAATG GCTGGACGCG 
ATGAAAATGG TTGCCAGGTT ACCAGGAGGA ATTCCGCCGG AATTTCGAAA GAAGTTGTGG 
CTGACACTGG CAGAACGTCA TCTAGAGCAG CGCGGCGTAG ATTGGAAGCA GGCTGAAAAG 
ATCTGCTTCA ACGAGTGGAG CAATCCTGAC GATGAAGAGC TTGGTATACA AATCGTTAAA 
GACCTACACA GAACGGGCTG CAGTCTGTTT TGCGGTGCAG CTGGCAGAGA CAATCAAGCA 
GTGCTTCGTC GAGTTCTGCT TGGTTTCGCC CGATGGAACA AGTCCGTCGG CTATTGCCAG 
GGCCTGAACG TGTTGGCCGC CCTCGTTTTG CAAGTAATGG ATCGCGCCGA GTCCGCCGCG 
GTGAAGGTGA TGATCTACTT GATAGAGGGT GTCCTGCCCG AGGGTTACTT CGCGGACAAT 
CTCCGTGGCC TGTCGGTCGA CATGGCGGTA TTTCGAGATC TCCTCCGGGG CAGACTGCCC 
AAGCTCTCGA AACATCTGGA GGCTCTGCAG AACGATGCTA AGGACAAGGC GACAGGCAGC 
AGTTACGAGC CACCTCTGAC GAATGTGTTC ACGATGCAAT GGTTCCTTAC TCTTTTCTGT 
CACTGTTTGC CACAAGAGGC TGTCCTGCGC GTATGGGACC TCATATTTCT CGAAGGAGAT 
GAGATATTGC TTAGAACCGC ACTGGCCATC TGGGAAGGAC TTTCCGATCG TATTATGACG 
GTGACATCGG CGGACGAGTT TTATAGTATA ATGGGAGTGC TGACAAGGGA GATGCTCGAG 
TTCACGGATA CTAATAACCT CATAAAGAAT ATCGTTAGTA TGGGATCTTT GCATGGTGTA 
ACGGGGCTAA GAGAGAAACA TAGATACAAT ATCACACCTT GGGCAAGAAA ATTGAGCGAC 
GACGAGGACA GCGACACGGA GGAGGATGAA AGGCTGGCCG TGGCTGCTGC GATGTTCAGT 
ATGGCACAGC GATTGAAGAA AGATCGTATA CCTCAAACGA TTGGAGCGCT GCAGGCAATG 
GCACCCAGCA GCGACCGAGA ACGGCTCGCT TTGGACATTA CCACTTTGAA ACAGCAGTAC 
GCCAAGCTAC GCGAACGTCA ACGACAAGCG CATATTATAC TTTCTGCTGC ATGTGCAAGG 
CAGACCATGG TACCACCTCC TACTTCTCAG GCCATGAATC ATCTTTTAGT GGGCAAAAGT 
GCGCTCGTGA GTGGGAAGAA TCGACCCCTG AGCCTACCTT CTGGTGCTGC AACGACGAAG 
ATAAGACCTA CGACGCTAAA TCAAAACCGA AGGGACAGAC AAGGCGTCAC GCTTCACTGG 
AAAGACACCA AAAAACCGAA GCAGAAACCC TCGAATTTAC CAGAAACGGT GGAATGTAGC 
GTTGTAACAA TGCAATCCAC GGAAGCTGAG CTGGCTTCTC CAAAGCGGAG TAACGGTGAC 
AGTGATAGCG ACAGCACATC GACGGAATTG TGCGACGAAC CGGACCGTCT CAGTGACGTT 
GACAGCGAAG ATCTGACGTC AGCATCCGAA TCCTGTGTTG TCAGTACGGA TGAGGAGAAA 
AGCTCTCGAA TCACCGGATC ACCGATTCGC GAGAAGCCAC TCGATCGTTC GTTTCTCGAA 
GAGAAGATGG AACCAATTAA CGCCGAAGAT TCGAAGGACT CGAAAAGCTC CGAGGATAAC 
CTTGGTGACA TATCAATCGC TAAAATCACC GACCAAATAA GACGATTATC AGCAGAGGAT 
GACAATAATT CCATGGTTCC TCAAAAATTT AGCGTGCAGA GCAAAGAATC GCCGGAAGAA 
CAATCGACGA AAGAATCGAA AAAAGAGAAA TCCATATCAG ATCTATCTAT AGATTACACT 
TCCGATAGTA ATATCATTAA AAAATCGTTA TTGGACTTGA ACGAGTACGA TTATCTAGGT 
TTCAGTGGCA GTAAGATCAG TTCGAAAGAA GATGAAGTAT TAGTGCACGA AAGGCATCGA 
TCTAAACAGA CAACAAACGA GATTGTAGAT ATACCTCGAA ACGAAGTTGC GACAATCAAG 
AAAGACCAAA CTGAAGACAA GATAACTACG ACTATGAAAC AGGATTCTTT AAACATATAC 
TTGGATTATT CAGCATCCTT GGACAAGAAA TACGATAGAA TCAAAGATAA ATCAAGCCTC 
GATGTAGAGA TAGAAAATGA AATTAGATCC GACCGATATA AAACATCCTT CGATTTCTCT 
AGTAGAATAT CTTTGGACTT GAAACCGTAT GAAACTTCAA AAGTAGGAAG TTTAACCAGT 
GACACTGCAG ACACAGTGAA TACTGAAAGA ACGCTTTATG GCAAGGCCTA CGTTGGTCAT 
CTTCCTATTT CACCTGTAAC AGTTGATCAA AAATTAAGCA GAGTGACACC CAGTATACAT 
ACAACGCATA ATACTACTGT ATTAACTCCT GAAACGCCTG AAATTGTACA TAAAACGTTA 
GAGAACACGT CATATAACGA TAGAATACAG ACGACTCAAA CGAAAATTTA TTCAGACCTA 
GTGAAGAGTC CTAAAACACC GGAAAGCAAC AAATCATTCA GTTCAGGTGA AACCATGGGA 
CCAGATTCGA AACCATCGAG TCTGACAGTT AGCCCAAGGA CGCCGGTTAG ATCAGAATCT 
CGAGATTTCC CTATGGACAA ATCCCTGTGT TCGTCAGTTA GCTCAGACTC GAAGACTTTG 
GTTCTTCATT CTGCAGATTC AGACAGTACG AGGGACAGCA CGAAAACCGA CGCGAAGAAA 
ATGTATGAGA TTTCCAATTC GGCGACACCT ATCAGCATGG ACTCGTTGCA GAAATCAGAA 
GCCAGCCCAA AACTAGTCGT GAGCAGCTCC AGTGAATCGT GTAGCCCGAG TAAATTAAAG 
TCTTCCGAGA GGTCGTTCAG TTCGGACCTG CAATCGCCAA TAAGTGCCAA CTCCATAAAG 
TATACTTCGA ATTTCAGTAG ACGGGGCCAA GATGACGTAT CAGGCTCGCC AATGGACTTG 
AGCAGCGCTA CCTCGCCGTT TTATCCGATC TCGAATCCGA ATCAAAAAAC TCCGACGTCC 
CCGTACAGTG CTTCTAGAAG AAGATCCAGT TTGGACAGCA ACGAAACGTC ACCTGAGCAG 
AAATCGACCA GTTCGAGGGA GTCGAACAAG TTTCTAGGTT ATCAGTCGAA ATTTGACTCC 
TCCATAAAGA CGTCTGATAT GAAAGATGTA GACGGTATAG CACGAAAAGG TGACACATTC 
GTCAGACCAG AGTTCATCAC GAGGAATGAA GATTCGAGTA TTAATTTGAC GGATAGAAGA 
ATCAATGTTA GTGATGTTAA GTATTATCAA ACAACGAGTA CGGATTACTA TCGCAAGGAT 
AAGGATATTT TTGATGACGG AGGGGATGAT CCGTCATCGG AGAAAGACGT GGTACCTTCT 
TTGCCTCAGG AGAAGCTAGA TAAACATTAT TTGAACTATC GATATAGAGA TAGATCGAAG 
TTGTTGGAAA GAAGTTCAAG CAGTTTGGAC AGTAGAAGGT ACGCTGATAT GAAGTCATCG 
GCTTCCACCG ATGAATTTTC AACGTCGATC GCGCAGAAGA GATCCAGTGA CATTTTAGAA 
GATATTAAAC ACTTGGAGGC GAAAGCAAAC GATGGGTCGT CGGATAATGG GATGTTGACG 
AAGAAGAGCA GTTATATTTG GGAGGATATG AAGAATTTAG AAGCCAGGAG GCAAGATACC 
TTTAGTGATT CAGCGAGGAA CTTTTCGAAA CATCGTCTGG AGGTACCGGA TATAAATATA 
ACCGGAGAAA GCAGAAAATC GTACGTAGTA CATCCGAAGA TCAATATCGA CGAAAATAGC 
GACAGCATAT TGAAGACCAT GGCCTGTGGT AAGAGTAACG GCACAGATGA TGGCAGGGAG 
TCGAAGTTGG GCGTGTGGAC GAAGGTGAAG CCTCGTAAAA AGAGTGACAA TGGGCGGAGG 
AACAGCGACA GGGCGTTGAA GATCATTCAA GAAAACTCCG CTATACTTCA GAAGATTCTT 
GCGTGTCAGG CAAAAAAGCG TCTGCCAGAC CTGGAGGAGA TATCGAAAGA AATTACCATT 
AGCCCTATAA ACGAGGAGAT TTCGAAGATC TTTAGTCCGA TATTAGAGAA AATGGGATTA 
AACGAACACG AGATTAACGA GGAATTGGCG AGAATCAATT TCAAGGATTT CGACAACATG 
ACCGCTACCA GTGTGTCCGA GTTTGATGCA AAGATTAACG AGGAATTATC GAAGCTTTCT 
CTGATCGACG AGACGGAGCA GATGGACCAC TTCGACGTGG ACGAGGTGAT ATCCCACGAA 
TACTTGAACA CGAGAGAAGC TCTAATAGAT CGTAAGATAA ACGAGGAATT GTCGAAATTA 
TTGGCAAACT ACGACGATCA TTCTCCGGCT AGTATGGTGA GTTTGGACAA AGGACCATCC 
AGCCAGAATA TTAGCGAGAT CGAAGGACTG GATCTGTCCA GTATTTCAAC TAACGTATTT 
TCCTATAAAT CATCCAATGA CTCCATTGAC ACGAGAAGCG ACTTAGGCTC GACGCAGGAA 
CCATCCATTC TCGTTGACAA GTTTCCGAAA TATACGGCAT ACGCCGTACC GAAGTATCAC 
GTGGACGACT CATCACCTAA AAGCGACATA GACATCTACA GAGAATTGGA GAAACTCGAT 
AAGATCTCTT CCGCACAGGT GTTACCAGAT CCGACTCTGG AGCTTCCACA GAAGGAATTG 
TCCTCGATTC CGTACGTTCC AAATACGTCG CCCTTCAAGG ATGTGTCACC ATTGAGGTAC 
ACTTCCAAGC CGGCTCAATA CGACACTTCA CCCCTGAAGA CTTCTTACGA TCCCTACAAG 
ACCTTTGACT TCAAGCCCAA GTTGTCACCT AAACAAGTTT CCACTAATCC ATTTGTGTCA 
GCTACGTATG AGAAACCTGT GGTGGATACC GCTTTCGAGT ACATTAACAA TAAACCAGAG 
ATCTCGATCA ATACGTATGA TCTACATTTG AAGAGTCCAA TGATGACGAA GGAATCGCTA 
GAGTTTCGTG TCAGATACGA CGATGATCCA GTCAGAGAAG TTAACACCGA TTACATGTCT 
CTCCAGCCAG AAAGTAGATC GATTACTACC CCGAGCAAGT CTCCTTATTT CAGCAATGGA 
AATACCGAAC CGTTAACGCC TACGAAATAT ATTTCTAAGG AGGAAAGAAT CTTGGATACT 
GGTGGTACCT CGTTTCTGAA CTATCCCAGC GAATCTTCCG ACCTTCTTCG TCGAAAATAC 
GACGCTTTGT CTCCTAAGGA GTACACTCAT ACCAAGAGTA CGACGGATTA TGATAGACAC 
TTAGGCACGT TACCTCCTAT AGAACCTGGA ACAGAGACAG GATCGTATTT ACCGACGAGA 
CACCGAGATT ATCATTCTCT ATCACCGAAT CGTCAGTTTC CTGATCACTT TCCTTCTACT 
ATCAATCATC ACTACACCTC GAAACTTTCA TCAGGTCTCT CTGACGAATC GAAACGACCG 
GTTTCTCATC CGGAATTCCC TGGCAAGATG AATGTTGGGA AATATGCAGA ATTACCCGAT 
AGAAGTTCCA GCAGTTACAA GAAATCATCT CTTAGCTTAG GTAACATAGG ACACTCGAGT 
AAAGGAGCTG ATAATAATTT CAACACTGTG ACCAGTCCAA AAACGCAGTT CAGTCCCTTT 
CCCGTTAGAA ACGCTCCTAG GAAGCCGAAA GAACTTACGC TGAAATTGGG CCTCTATTCG 
CCCAAAGGTT CTGATACAGG ACAGTTAAAA AGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012243270.1
CDS	231..6089
Translation	MRFSLTPQPGESGFIQWLDAMKMVARLPGGIPPEFRKKLWLTLAERHLEQRGVDWKQAEKICFNEWSNPDDEELGIQIVKDLHRTGCSLFCGAAGRDNQAVLRRVLLGFARWNKSVGYCQGLNVLAALVLQVMDRAESAAVKVMIYLIEGVLPEGYFADNLRGLSVDMAVFRDLLRGRLPKLSKHLEALQNDAKDKATGSSYEPPLTNVFTMQWFLTLFCHCLPQEAVLRVWDLIFLEGDEILLRTALAIWEGLSDRIMTVTSADEFYSIMGVLTREMLEFTDTNNLIKNIVSMGSLHGVTGLREKHRYNITPWARKLSDDEDSDTEEDERLAVAAAMFSMAQRLKKDRIPQTIGALQAMAPSSDRERLALDITTLKQQYAKLRERQRQAHIILSAACARQTMVPPPTSQAMNHLLVGKSALVSGKNRPLSLPSGAATTKIRPTTLNQNRRDRQGVTLHWKDTKKPKQKPSNLPETVECSVVTMQSTEAELASPKRSNGDSDSDSTSTELCDEPDRLSDVDSEDLTSASESCVVSTDEEKSSRITGSPIREKPLDRSFLEEKMEPINAEDSKDSKSSEDNLGDISIAKITDQIRRLSAEDDNNSMVPQKFSVQSKESPEEQSTKESKKEKSISDLSIDYTSDSNIIKKSLLDLNEYDYLGFSGSKISSKEDEVLVHERHRSKQTTNEIVDIPRNEVATIKKDQTEDKITTTMKQDSLNIYLDYSASLDKKYDRIKDKSSLDVEIENEIRSDRYKTSFDFSSRISLDLKPYETSKVGSLTSDTADTVNTERTLYGKAYVGHLPISPVTVDQKLSRVTPSIHTTHNTTVLTPETPEIVHKTLENTSYNDRIQTTQTKIYSDLVKSPKTPESNKSFSSGETMGPDSKPSSLTVSPRTPVRSESRDFPMDKSLCSSVSSDSKTLVLHSADSDSTRDSTKTDAKKMYEISNSATPISMDSLQKSEASPKLVVSSSSESCSPSKLKSSERSFSSDLQSPISANSIKYTSNFSRRGQDDVSGSPMDLSSATSPFYPISNPNQKTPTSPYSASRRRSSLDSNETSPEQKSTSSRESNKFLGYQSKFDSSIKTSDMKDVDGIARKGDTFVRPEFITRNEDSSINLTDRRINVSDVKYYQTTSTDYYRKDKDIFDDGGDDPSSEKDVVPSLPQEKLDKHYLNYRYRDRSKLLERSSSSLDSRRYADMKSSASTDEFSTSIAQKRSSDILEDIKHLEAKANDGSSDNGMLTKKSSYIWEDMKNLEARRQDTFSDSARNFSKHRLEVPDINITGESRKSYVVHPKINIDENSDSILKTMACGKSNGTDDGRESKLGVWTKVKPRKKSDNGRRNSDRALKIIQENSAILQKILACQAKKRLPDLEEISKEITISPINEEISKIFSPILEKMGLNEHEINEELARINFKDFDNMTATSVSEFDAKINEELSKLSLIDETEQMDHFDVDEVISHEYLNTREALIDRKINEELSKLLANYDDHSPASMVSLDKGPSSQNISEIEGLDLSSISTNVFSYKSSNDSIDTRSDLGSTQEPSILVDKFPKYTAYAVPKYHVDDSSPKSDIDIYRELEKLDKISSAQVLPDPTLELPQKELSSIPYVPNTSPFKDVSPLRYTSKPAQYDTSPLKTSYDPYKTFDFKPKLSPKQVSTNPFVSATYEKPVVDTAFEYINNKPEISINTYDLHLKSPMMTKESLEFRVRYDDDPVREVNTDYMSLQPESRSITTPSKSPYFSNGNTEPLTPTKYISKEERILDTGGTSFLNYPSESSDLLRRKYDALSPKEYTHTKSTTDYDRHLGTLPPIEPGTETGSYLPTRHRDYHSLSPNRQFPDHFPSTINHHYTSKLSSGLSDESKRPVSHPEFPGKMNVGKYAELPDRSSSSYKKSSLSLGNIGHSSKGADNNFNTVTSPKTQFSPFPVRNAPRKPKELTLKLGLYSPKGSDTGQLKRS

Target ORF information:

RefSeq Version	XM_012387847.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012387847.1

ORF Insert Sequence:

ATGAGATTTA GCCTGACGCC GCAACCAGGA GAAAGTGGTT TTATACAATG GCTGGACGCG 
ATGAAAATGG TTGCCAGGTT ACCAGGAGGA ATTCCGCCGG AATTTCGAAA GAAGTTGTGG 
CTGACACTGG CAGAACGTCA TCTAGAGCAG CGCGGCGTAG ATTGGAAGCA GGCTGAAAAG 
ATCTGCTTCA ACGAGTGGAG CAATCCTGAC GATGAAGAGC TTGGTATACA AATCGTTAAA 
GACCTACACA GAACGGGCTG CAGTCTGTTT TGCGGTGCAG CTGGCAGAGA CAATCAAGCA 
GTGCTTCGTC GAGTTCTGCT TGGTTTCGCC CGATGGAACA AGTCCGTCGG CTATTGCCAG 
GGCCTGAACG TGTTGGCCGC CCTCGTTTTG CAAGTAATGG ATCGCGCCGA GTCCGCCGCG 
GTGAAGGTGA TGATCTACTT GATAGAGGGT GTCCTGCCCG AGGGTTACTT CGCGGACAAT 
CTCCGTGGCC TGTCGGTCGA CATGGCGGTA TTTCGAGATC TCCTCCGGGG CAGACTGCCC 
AAGCTCTCGA AACATCTGGA GGCTCTGCAG AACGATGCTA AGGACAAGGC GACAGGCAGC 
AGTTACGAGC CACCTCTGAC GAATGTGTTC ACGATGCAAT GGTTCCTTAC TCTTTTCTGT 
CACTGTTTGC CACAAGAGGC TGTCCTGCGC GTATGGGACC TCATATTTCT CGAAGGAGAT 
GAGATATTGC TTAGAACCGC ACTGGCCATC TGGGAAGGAC TTTCCGATCG TATTATGACG 
GTGACATCGG CGGACGAGTT TTATAGTATA ATGGGAGTGC TGACAAGGGA GATGCTCGAG 
TTCACGGATA CTAATAACCT CATAAAGAAT ATCGTTAGTA TGGGATCTTT GCATGGTGTA 
ACGGGGCTAA GAGAGAAACA TAGATACAAT ATCACACCTT GGGCAAGAAA ATTGAGCGAC 
GACGAGGACA GCGACACGGA GGAGGATGAA AGGCTGGCCG TGGCTGCTGC GATGTTCAGT 
ATGGCACAGC GATTGAAGAA AGATCGTATA CCTCAAACGA TTGGAGCGCT GCAGGCAATG 
GCACCCAGCA GCGACCGAGA ACGGCTCGCT TTGGACATTA CCACTTTGAA ACAGCAGTAC 
GCCAAGCTAC GCGAACGTCA ACGACAAGCG CATATTATAC TTTCTGCTGC ATGTGCAAGG 
CAGACCATGG TACCACCTCC TACTTCTCAG GCCATGAATC ATCTTTTAGT GGGCAAAAGT 
GCGCTCGTGA GTGGGAAGAA TCGACCCCTG AGCCTACCTT CTGGTGCTGC AACGACGAAG 
ATAAGACCTA CGACGCTAAA TCAAAACCGA AGGGACAGAC AAGGCGTCAC GCTTCACTGG 
AAAGACACCA AAAAACCGAA GCAGAAACCC TCGAATTTAC CAGAAACGGT GGAATGTAGC 
GTTGTAACAA TGCAATCCAC GGAAGCTGAG CTGGCTTCTC CAAAGCGGAG TAACGGTGAC 
AGTGATAGCG ACAGCACATC GACGGAATTG TGCGACGAAC CGGACCGTCT CAGTGACGTT 
GACAGCGAAG ATCTGACGTC AGCATCCGAA TCCTGTGTTG TCAGTACGGA TGAGGAGAAA 
AGCTCTCGAA TCACCGGATC ACCGATTCGC GAGAAGCCAC TCGATCGTTC GTTTCTCGAA 
GAGAAGATGG AACCAATTAA CGCCGAAGAT TCGAAGGACT CGAAAAGCTC CGAGGATAAC 
CTTGGTGACA TATCAATCGC TAAAATCACC GACCAAATAA GACGATTATC AGCAGAGGAT 
GACAATAATT CCATGGTTCC TCAAAAATTT AGCGTGCAGA GCAAAGAATC GCCGGAAGAA 
CAATCGACGA AAGAATCGAA AAAAGAGAAA TCCATATCAG ATCTATCTAT AGATTACACT 
TCCGATAGTA ATATCATTAA AAAATCGTTA TTGGACTTGA ACGAGTACGA TTATCTAGGT 
TTCAGTGGCA GTAAGATCAG TTCGAAAGAA GATGAAGTAT TAGTGCACGA AAGGCATCGA 
TCTAAACAGA CAACAAACGA GATTGTAGAT ATACCTCGAA ACGAAGTTGC GACAATCAAG 
AAAGACCAAA CTGAAGACAA GATAACTACG ACTATGAAAC AGGATTCTTT AAACATATAC 
TTGGATTATT CAGCATCCTT GGACAAGAAA TACGATAGAA TCAAAGATAA ATCAAGCCTC 
GATGTAGAGA TAGAAAATGA AATTAGATCC GACCGATATA AAACATCCTT CGATTTCTCT 
AGTAGAATAT CTTTGGACTT GAAACCGTAT GAAACTTCAA AAGTAGGAAG TTTAACCAGT 
GACACTGCAG ACACAGTGAA TACTGAAAGA ACGCTTTATG GCAAGGCCTA CGTTGGTCAT 
CTTCCTATTT CACCTGTAAC AGTTGATCAA AAATTAAGCA GAGTGACACC CAGTATACAT 
ACAACGCATA ATACTACTGT ATTAACTCCT GAAACGCCTG AAATTGTACA TAAAACGTTA 
GAGAACACGT CATATAACGA TAGAATACAG ACGACTCAAA CGAAAATTTA TTCAGACCTA 
GTGAAGAGTC CTAAAACACC GGAAAGCAAC AAATCATTCA GTTCAGGTGA AACCATGGGA 
CCAGATTCGA AACCATCGAG TCTGACAGTT AGCCCAAGGA CGCCGGTTAG ATCAGAATCT 
CGAGATTTCC CTATGGACAA ATCCCTGTGT TCGTCAGTTA GCTCAGACTC GAAGACTTTG 
GTTCTTCATT CTGCAGATTC AGACAGTACG AGGGACAGCA CGAAAACCGA CGCGAAGAAA 
ATGTATGAGA TTTCCAATTC GGCGACACCT ATCAGCATGG ACTCGTTGCA GAAATCAGAA 
GCCAGCCCAA AACTAGTCGT GAGCAGCTCC AGTGAATCGT GTAGCCCGAG TAAATTAAAG 
TCTTCCGAGA GGTCGTTCAG TTCGGACCTG CAATCGCCAA TAAGTGCCAA CTCCATAAAG 
TATACTTCGA ATTTCAGTAG ACGGGGCCAA GATGACGTAT CAGGCTCGCC AATGGACTTG 
AGCAGCGCTA CCTCGCCGTT TTATCCGATC TCGAATCCGA ATCAAAAAAC TCCGACGTCC 
CCGTACAGTG CTTCTAGAAG AAGATCCAGT TTGGACAGCA ACGAAACGTC ACCTGAGCAG 
AAATCGACCA GTTCGAGGGA GTCGAACAAG TTTCTAGGTT ATCAGTCGAA ATTTGACTCC 
TCCATAAAGA CGTCTGATAT GAAAGATGTA GACGGTATAG CACGAAAAGG TGACACATTC 
GTCAGACCAG AGTTCATCAC GAGGAATGAA GATTCGAGTA TTAATTTGAC GGATAGAAGA 
ATCAATGTTA GTGATGTTAA GTATTATCAA ACAACGAGTA CGGATTACTA TCGCAAGGAT 
AAGGATATTT TTGATGACGG AGGGGATGAT CCGTCATCGG AGAAAGACGT GGTACCTTCT 
TTGCCTCAGG AGAAGCTAGA TAAACATTAT TTGAACTATC GATATAGAGA TAGATCGAAG 
TTGTTGGAAA GAAGTTCAAG CAGTTTGGAC AGTAGAAGGT ACGCTGATAT GAAGTCATCG 
GCTTCCACCG ATGAATTTTC AACGTCGATC GCGCAGAAGA GATCCAGTGA CATTTTAGAA 
GATATTAAAC ACTTGGAGGC GAAAGCAAAC GATGGGTCGT CGGATAATGG GATGTTGACG 
AAGAAGAGCA GTTATATTTG GGAGGATATG AAGAATTTAG AAGCCAGGAG GCAAGATACC 
TTTAGTGATT CAGCGAGGAA CTTTTCGAAA CATCGTCTGG AGGTACCGGA TATAAATATA 
ACCGGAGAAA GCAGAAAATC GTACGTAGTA CATCCGAAGA TCAATATCGA CGAAAATAGC 
GACAGCATAT TGAAGACCAT GGCCTGTGGT AAGAGTAACG GCACAGATGA TGGCAGGGAG 
TCGAAGTTGG GCGTGTGGAC GAAGGTGAAG CCTCGTAAAA AGAGTGACAA TGGGCGGAGG 
AACAGCGACA GGGCGTTGAA GATCATTCAA GAAAACTCCG CTATACTTCA GAAGATTCTT 
GCGTGTCAGG CAAAAAAGCG TCTGCCAGAC CTGGAGGAGA TATCGAAAGA AATTACCATT 
AGCCCTATAA ACGAGGAGAT TTCGAAGATC TTTAGTCCGA TATTAGAGAA AATGGGATTA 
AACGAACACG AGATTAACGA GGAATTGGCG AGAATCAATT TCAAGGATTT CGACAACATG 
ACCGCTACCA GTGTGTCCGA GTTTGATGCA AAGATTAACG AGGAATTATC GAAGCTTTCT 
CTGATCGACG AGACGGAGCA GATGGACCAC TTCGACGTGG ACGAGGTGAT ATCCCACGAA 
TACTTGAACA CGAGAGAAGC TCTAATAGAT CGTAAGATAA ACGAGGAATT GTCGAAATTA 
TTGGCAAACT ACGACGATCA TTCTCCGGCT AGTATGGTGA GTTTGGACAA AGGACCATCC 
AGCCAGAATA TTAGCGAGAT CGAAGGACTG GATCTGTCCA GTATTTCAAC TAACGTATTT 
TCCTATAAAT CATCCAATGA CTCCATTGAC ACGAGAAGCG ACTTAGGCTC GACGCAGGAA 
CCATCCATTC TCGTTGACAA GTTTCCGAAA TATACGGCAT ACGCCGTACC GAAGTATCAC 
GTGGACGACT CATCACCTAA AAGCGACATA GACATCTACA GAGAATTGGA GAAACTCGAT 
AAGATCTCTT CCGCACAGGT GTTACCAGAT CCGACTCTGG AGCTTCCACA GAAGGAATTG 
TCCTCGATTC CGTACGTTCC AAATACGTCG CCCTTCAAGG ATGTGTCACC ATTGAGGTAC 
ACTTCCAAGC CGGCTCAATA CGACACTTCA CCCCTGAAGA CTTCTTACGA TCCCTACAAG 
ACCTTTGACT TCAAGCCCAA GTTGTCACCT AAACAAGTTT CCACTAATCC ATTTGTGTCA 
GCTACGTATG AGAAACCTGT GGTGGATACC GCTTTCGAGT ACATTAACAA TAAACCAGAG 
ATCTCGATCA ATACGTATGA TCTACATTTG AAGAGTCCAA TGATGACGAA GGAATCGCTA 
GAGTTTCGTG TCAGATACGA CGATGATCCA GTCAGAGAAG TTAACACCGA TTACATGTCT 
CTCCAGCCAG AAAGTAGATC GATTACTACC CCGAGCAAGT CTCCTTATTT CAGCAATGGA 
AATACCGAAC CGTTAACGCC TACGAAATAT ATTTCTAAGG AGGAAAGAAT CTTGGATACT 
GGTGGTACCT CGTTTCTGAA CTATCCCAGC GAATCTTCCG ACCTTCTTCG TCGAAAATAC 
GACGCTTTGT CTCCTAAGGA GTACACTCAT ACCAAGAGTA CGACGGATTA TGATAGACAC 
TTAGGCACGT TACCTCCTAT AGAACCTGGA ACAGAGACAG GATCGTATTT ACCGACGAGA 
CACCGAGATT ATCATTCTCT ATCACCGAAT CGTCAGTTTC CTGATCACTT TCCTTCTACT 
ATCAATCATC ACTACACCTC GAAACTTTCA TCAGGTCTCT CTGACGAATC GAAACGACCG 
GTTTCTCATC CGGAATTCCC TGGCAAGATG AATGTTGGGA AATATGCAGA ATTACCCGAT 
AGAAGTTCCA GCAGTTACAA GAAATCATCT CTTAGCTTAG GTAACATAGG ACACTCGAGT 
AAAGGAGCTG ATAATAATTT CAACACTGTG ACCAGTCCAA AAACGCAGTT CAGTCCCTTT 
CCCGTTAGAA ACGCTCCTAG GAAGCCGAAA GAACTTACGC TGAAATTGGG CCTCTATTCG 
CCCAAAGGTT CTGATACAGG ACAGTTAAAA AGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100749006 ( XM_003490922.3 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_003490970.1 Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X1, mRNA.

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CloneID	OBo106112
Clone ID Related Accession (Same CDS sequence)	XM_003490922.3 , XM_003490922.2
Accession Version	XM_003490922.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	uncharacterized protein LOC100749006 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176906.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 19, 2018 this sequence version replaced XM_003490922.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTA ACGTGATGCC TCTGAAGAAG TACGCGGAAA TTAGTTACCG CGTGAAATGT 
GTGGCCGTGG AAAATGAGCG CGTGTTTGGT GACGTGTACG TCACACAACA AACACGAAAT 
TTGCTGTATT GCGCAGATGG ATATCAGTTT CCCTCGTTGT ATAACAGAAG CAGACGCAAT 
CTGATTCAGA AATCATGGGA AGGTCCGGAT TTCATGGAAC TGAATCGGAA AAAGCCGATC 
AGAAGGGCTA GTTGCACGCC GCCCAAAGTG GAGAGAACAA ATTTGAAGAG GTCGAAATCT 
TTGGGTGACA CGTGGGACGT AATCGAAACT GATTGCAAAA ATGCTAACAA TTCGAAAGAT 
GAAAATTACA TTTGCAAGCC AAAGACATAT CCATCACCGT TACCGAATCC ACCCGAGAAC 
AAAAAAGGAA CTGTGTCTTC GTTAGTTGAT CAATTGTTGC TAGATATTTA TGGATTGCCA 
GACGATGACA GAAGACGGTC AGAAAGTGAC TCGACAGCCT CATCCCTGAA GATACGTCCG 
CAACATCAGC ACCTGCAGAA GGCGCGTTTA CTCTGGAAAA GTGAGCACGA GCTTCAAGTT 
TTAGTGCTGA ATTTACGCGA CCATATTAAT CACACTGGTG AAATACTCAT TCGACAGTTG 
CGTCGTAAAG ATCATCTCGC CACCAAATGT GAGAAACTAT GCGCCATAAT TACGGCTCAC 
CTTCTGGCAG TCAGCCCAAA ATGCGTCGAA GACACGAAGA TGAGATTTAG CCTGACGCCG 
CAACCAGGAG AAAGTGGTTT TATACAATGG CTGGACGCGA TGAAAATGGT TGCCAGGTTA 
CCAGGAGGAA TTCCGCCGGA ATTTCGAAAG AAGTTGTGGC TGACACTGGC AGAACGTCAT 
CTAGAGCAGC GCGGCGTAGA TTGGAAGCAG GCTGAAAAGA TCTGCTTCAA CGAGTGGAGC 
AATCCTGACG ATGAAGAGCT TGGTATACAA ATCGTTAAAG ACCTACACAG AACGGGCTGC 
AGTCTGTTTT GCGGTGCAGC TGGCAGAGAC AATCAAGCAG TGCTTCGTCG AGTTCTGCTT 
GGTTTCGCCC GATGGAACAA GTCCGTCGGC TATTGCCAGG GCCTGAACGT GTTGGCCGCC 
CTCGTTTTGC AAGTAATGGA TCGCGCCGAG TCCGCCGCGG TGAAGGTGAT GATCTACTTG 
ATAGAGGGTG TCCTGCCCGA GGGTTACTTC GCGGACAATC TCCGTGGCCT GTCGGTCGAC 
ATGGCGGTAT TTCGAGATCT CCTCCGGGGC AGACTGCCCA AGCTCTCGAA ACATCTGGAG 
GCTCTGCAGA ACGATGCTAA GGACAAGGCG ACAGGCAGCA GTTACGAGCC ACCTCTGACG 
AATGTGTTCA CGATGCAATG GTTCCTTACT CTTTTCTGTC ACTGTTTGCC ACAAGAGGCT 
GTCCTGCGCG TATGGGACCT CATATTTCTC GAAGGAGATG AGATATTGCT TAGAACCGCA 
CTGGCCATCT GGGAAGGACT TTCCGATCGT ATTATGACGG TGACATCGGC GGACGAGTTT 
TATAGTATAA TGGGAGTGCT GACAAGGGAG ATGCTCGAGT TCACGGATAC TAATAACCTC 
ATAAAGAATA TCGTTAGTAT GGGATCTTTG CATGGTGTAA CGGGGCTAAG AGAGAAACAT 
AGATACAATA TCACACCTTG GGCAAGAAAA TTGAGCGACG ACGAGGACAG CGACACGGAG 
GAGGATGAAA GGCTGGCCGT GGCTGCTGCG ATGTTCAGTA TGGCACAGCG ATTGAAGAAA 
GATCGTATAC CTCAAACGAT TGGAGCGCTG CAGGCAATGG CACCCAGCAG CGACCGAGAA 
CGGCTCGCTT TGGACATTAC CACTTTGAAA CAGCAGTACG CCAAGCTACG CGAACGTCAA 
CGACAAGCGC ATATTATACT TTCTGCTGCA TGTGCAAGGC AGACCATGGT ACCACCTCCT 
ACTTCTCAGG CCATGAATCA TCTTTTAGTG GGCAAAAGTG CGCTCGTGAG TGGGAAGAAT 
CGACCCCTGA GCCTACCTTC TGGTGCTGCA ACGACGAAGA TAAGACCTAC GACGCTAAAT 
CAAAACCGAA GGGACAGACA AGGCGTCACG CTTCACTGGA AAGACACCAA AAAACCGAAG 
CAGAAACCCT CGAATTTACC AGAAACGGTG GAATGTAGCG TTGTAACAAT GCAATCCACG 
GAAGCTGAGC TGGCTTCTCC AAAGCGGAGT AACGGTGACA GTGATAGCGA CAGCACATCG 
ACGGAATTGT GCGACGAACC GGACCGTCTC AGTGACGTTG ACAGCGAAGA TCTGACGTCA 
GCATCCGAAT CCTGTGTTGT CAGTACGGAT GAGGAGAAAA GCTCTCGAAT CACCGGATCA 
CCGATTCGCG AGAAGCCACT CGATCGTTCG TTTCTCGAAG AGAAGATGGA ACCAATTAAC 
GCCGAAGATT CGAAGGACTC GAAAAGCTCC GAGGATAACC TTGGTGACAT ATCAATCGCT 
AAAATCACCG ACCAAATAAG ACGATTATCA GCAGAGGATG ACAATAATTC CATGGTTCCT 
CAAAAATTTA GCGTGCAGAG CAAAGAATCG CCGGAAGAAC AATCGACGAA AGAATCGAAA 
AAAGAGAAAT CCATATCAGA TCTATCTATA GATTACACTT CCGATAGTAA TATCATTAAA 
AAATCGTTAT TGGACTTGAA CGAGTACGAT TATCTAGGTT TCAGTGGCAG TAAGATCAGT 
TCGAAAGAAG ATGAAGTATT AGTGCACGAA AGGCATCGAT CTAAACAGAC AACAAACGAG 
ATTGTAGATA TACCTCGAAA CGAAGTTGCG ACAATCAAGA AAGACCAAAC TGAAGACAAG 
ATAACTACGA CTATGAAACA GGATTCTTTA AACATATACT TGGATTATTC AGCATCCTTG 
GACAAGAAAT ACGATAGAAT CAAAGATAAA TCAAGCCTCG ATGTAGAGAT AGAAAATGAA 
ATTAGATCCG ACCGATATAA AACATCCTTC GATTTCTCTA GTAGAATATC TTTGGACTTG 
AAACCGTATG AAACTTCAAA AGTAGGAAGT TTAACCAGTG ACACTGCAGA CACAGTGAAT 
ACTGAAAGAA CGCTTTATGG CAAGGCCTAC GTTGGTCATC TTCCTATTTC ACCTGTAACA 
GTTGATCAAA AATTAAGCAG AGTGACACCC AGTATACATA CAACGCATAA TACTACTGTA 
TTAACTCCTG AAACGCCTGA AATTGTACAT AAAACGTTAG AGAACACGTC ATATAACGAT 
AGAATACAGA CGACTCAAAC GAAAATTTAT TCAGACCTAG TGAAGAGTCC TAAAACACCG 
GAAAGCAACA AATCATTCAG TTCAGGTGAA ACCATGGGAC CAGATTCGAA ACCATCGAGT 
CTGACAGTTA GCCCAAGGAC GCCGGTTAGA TCAGAATCTC GAGATTTCCC TATGGACAAA 
TCCCTGTGTT CGTCAGTTAG CTCAGACTCG AAGACTTTGG TTCTTCATTC TGCAGATTCA 
GACAGTACGA GGGACAGCAC GAAAACCGAC GCGAAGAAAA TGTATGAGAT TTCCAATTCG 
GCGACACCTA TCAGCATGGA CTCGTTGCAG AAATCAGAAG CCAGCCCAAA ACTAGTCGTG 
AGCAGCTCCA GTGAATCGTG TAGCCCGAGT AAATTAAAGT CTTCCGAGAG GTCGTTCAGT 
TCGGACCTGC AATCGCCAAT AAGTGCCAAC TCCATAAAGT ATACTTCGAA TTTCAGTAGA 
CGGGGCCAAG ATGACGTATC AGGCTCGCCA ATGGACTTGA GCAGCGCTAC CTCGCCGTTT 
TATCCGATCT CGAATCCGAA TCAAAAAACT CCGACGTCCC CGTACAGTGC TTCTAGAAGA 
AGATCCAGTT TGGACAGCAA CGAAACGTCA CCTGAGCAGA AATCGACCAG TTCGAGGGAG 
TCGAACAAGT TTCTAGGTTA TCAGTCGAAA TTTGACTCCT CCATAAAGAC GTCTGATATG 
AAAGATGTAG ACGGTATAGC ACGAAAAGGT GACACATTCG TCAGACCAGA GTTCATCACG 
AGGAATGAAG ATTCGAGTAT TAATTTGACG GATAGAAGAA TCAATGTTAG TGATGTTAAG 
TATTATCAAA CAACGAGTAC GGATTACTAT CGCAAGGATA AGGATATTTT TGATGACGGA 
GGGGATGATC CGTCATCGGA GAAAGACGTG GTACCTTCTT TGCCTCAGGA GAAGCTAGAT 
AAACATTATT TGAACTATCG ATATAGAGAT AGATCGAAGT TGTTGGAAAG AAGTTCAAGC 
AGTTTGGACA GTAGAAGGTA CGCTGATATG AAGTCATCGG CTTCCACCGA TGAATTTTCA 
ACGTCGATCG CGCAGAAGAG ATCCAGTGAC ATTTTAGAAG ATATTAAACA CTTGGAGGCG 
AAAGCAAACG ATGGGTCGTC GGATAATGGG ATGTTGACGA AGAAGAGCAG TTATATTTGG 
GAGGATATGA AGAATTTAGA AGCCAGGAGG CAAGATACCT TTAGTGATTC AGCGAGGAAC 
TTTTCGAAAC ATCGTCTGGA GGTACCGGAT ATAAATATAA CCGGAGAAAG CAGAAAATCG 
TACGTAGTAC ATCCGAAGAT CAATATCGAC GAAAATAGCG ACAGCATATT GAAGACCATG 
GCCTGTGGTA AGAGTAACGG CACAGATGAT GGCAGGGAGT CGAAGTTGGG CGTGTGGACG 
AAGGTGAAGC CTCGTAAAAA GAGTGACAAT GGGCGGAGGA ACAGCGACAG GGCGTTGAAG 
ATCATTCAAG AAAACTCCGC TATACTTCAG AAGATTCTTG CGTGTCAGGC AAAAAAGCGT 
CTGCCAGACC TGGAGGAGAT ATCGAAAGAA ATTACCATTA GCCCTATAAA CGAGGAGATT 
TCGAAGATCT TTAGTCCGAT ATTAGAGAAA ATGGGATTAA ACGAACACGA GATTAACGAG 
GAATTGGCGA GAATCAATTT CAAGGATTTC GACAACATGA CCGCTACCAG TGTGTCCGAG 
TTTGATGCAA AGATTAACGA GGAATTATCG AAGCTTTCTC TGATCGACGA GACGGAGCAG 
ATGGACCACT TCGACGTGGA CGAGGTGATA TCCCACGAAT ACTTGAACAC GAGAGAAGCT 
CTAATAGATC GTAAGATAAA CGAGGAATTG TCGAAATTAT TGGCAAACTA CGACGATCAT 
TCTCCGGCTA GTATGGTGAG TTTGGACAAA GGACCATCCA GCCAGAATAT TAGCGAGATC 
GAAGGACTGG ATCTGTCCAG TATTTCAACT AACGTATTTT CCTATAAATC ATCCAATGAC 
TCCATTGACA CGAGAAGCGA CTTAGGCTCG ACGCAGGAAC CATCCATTCT CGTTGACAAG 
TTTCCGAAAT ATACGGCATA CGCCGTACCG AAGTATCACG TGGACGACTC ATCACCTAAA 
AGCGACATAG ACATCTACAG AGAATTGGAG AAACTCGATA AGATCTCTTC CGCACAGGTG 
TTACCAGATC CGACTCTGGA GCTTCCACAG AAGGAATTGT CCTCGATTCC GTACGTTCCA 
AATACGTCGC CCTTCAAGGA TGTGTCACCA TTGAGGTACA CTTCCAAGCC GGCTCAATAC 
GACACTTCAC CCCTGAAGAC TTCTTACGAT CCCTACAAGA CCTTTGACTT CAAGCCCAAG 
TTGTCACCTA AACAAGTTTC CACTAATCCA TTTGTGTCAG CTACGTATGA GAAACCTGTG 
GTGGATACCG CTTTCGAGTA CATTAACAAT AAACCAGAGA TCTCGATCAA TACGTATGAT 
CTACATTTGA AGAGTCCAAT GATGACGAAG GAATCGCTAG AGTTTCGTGT CAGATACGAC 
GATGATCCAG TCAGAGAAGT TAACACCGAT TACATGTCTC TCCAGCCAGA AAGTAGATCG 
ATTACTACCC CGAGCAAGTC TCCTTATTTC AGCAATGGAA ATACCGAACC GTTAACGCCT 
ACGAAATATA TTTCTAAGGA GGAAAGAATC TTGGATACTG GTGGTACCTC GTTTCTGAAC 
TATCCCAGCG AATCTTCCGA CCTTCTTCGT CGAAAATACG ACGCTTTGTC TCCTAAGGAG 
TACACTCATA CCAAGAGTAC GACGGATTAT GATAGACACT TAGGCACGTT ACCTCCTATA 
GAACCTGGAA CAGAGACAGG ATCGTATTTA CCGACGAGAC ACCGAGATTA TCATTCTCTA 
TCACCGAATC GTCAGTTTCC TGATCACTTT CCTTCTACTA TCAATCATCA CTACACCTCG 
AAACTTTCAT CAGGTCTCTC TGACGAATCG AAACGACCGG TTTCTCATCC GGAATTCCCT 
GGCAAGATGA ATGTTGGGAA ATATGCAGAA TTACCCGATA GAAGTTCCAG CAGTTACAAG 
AAATCATCTC TTAGCTTAGG TAACATAGGA CACTCGAGTA AAGGAGCTGA TAATAATTTC 
AACACTGTGA CCAGTCCAAA AACGCAGTTC AGTCCCTTTC CCGTTAGAAA CGCTCCTAGG 
AAGCCGAAAG AACTTACGCT GAAATTGGGC CTCTATTCGC CCAAAGGTTC TGATACAGGA 
CAGTTAAAAA GGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003490970.1
CDS	1744..8361
Translation	MSCNVMPLKKYAEISYRVKCVAVENERVFGDVYVTQQTRNLLYCADGYQFPSLYNRSRRNLIQKSWEGPDFMELNRKKPIRRASCTPPKVERTNLKRSKSLGDTWDVIETDCKNANNSKDENYICKPKTYPSPLPNPPENKKGTVSSLVDQLLLDIYGLPDDDRRRSESDSTASSLKIRPQHQHLQKARLLWKSEHELQVLVLNLRDHINHTGEILIRQLRRKDHLATKCEKLCAIITAHLLAVSPKCVEDTKMRFSLTPQPGESGFIQWLDAMKMVARLPGGIPPEFRKKLWLTLAERHLEQRGVDWKQAEKICFNEWSNPDDEELGIQIVKDLHRTGCSLFCGAAGRDNQAVLRRVLLGFARWNKSVGYCQGLNVLAALVLQVMDRAESAAVKVMIYLIEGVLPEGYFADNLRGLSVDMAVFRDLLRGRLPKLSKHLEALQNDAKDKATGSSYEPPLTNVFTMQWFLTLFCHCLPQEAVLRVWDLIFLEGDEILLRTALAIWEGLSDRIMTVTSADEFYSIMGVLTREMLEFTDTNNLIKNIVSMGSLHGVTGLREKHRYNITPWARKLSDDEDSDTEEDERLAVAAAMFSMAQRLKKDRIPQTIGALQAMAPSSDRERLALDITTLKQQYAKLRERQRQAHIILSAACARQTMVPPPTSQAMNHLLVGKSALVSGKNRPLSLPSGAATTKIRPTTLNQNRRDRQGVTLHWKDTKKPKQKPSNLPETVECSVVTMQSTEAELASPKRSNGDSDSDSTSTELCDEPDRLSDVDSEDLTSASESCVVSTDEEKSSRITGSPIREKPLDRSFLEEKMEPINAEDSKDSKSSEDNLGDISIAKITDQIRRLSAEDDNNSMVPQKFSVQSKESPEEQSTKESKKEKSISDLSIDYTSDSNIIKKSLLDLNEYDYLGFSGSKISSKEDEVLVHERHRSKQTTNEIVDIPRNEVATIKKDQTEDKITTTMKQDSLNIYLDYSASLDKKYDRIKDKSSLDVEIENEIRSDRYKTSFDFSSRISLDLKPYETSKVGSLTSDTADTVNTERTLYGKAYVGHLPISPVTVDQKLSRVTPSIHTTHNTTVLTPETPEIVHKTLENTSYNDRIQTTQTKIYSDLVKSPKTPESNKSFSSGETMGPDSKPSSLTVSPRTPVRSESRDFPMDKSLCSSVSSDSKTLVLHSADSDSTRDSTKTDAKKMYEISNSATPISMDSLQKSEASPKLVVSSSSESCSPSKLKSSERSFSSDLQSPISANSIKYTSNFSRRGQDDVSGSPMDLSSATSPFYPISNPNQKTPTSPYSASRRRSSLDSNETSPEQKSTSSRESNKFLGYQSKFDSSIKTSDMKDVDGIARKGDTFVRPEFITRNEDSSINLTDRRINVSDVKYYQTTSTDYYRKDKDIFDDGGDDPSSEKDVVPSLPQEKLDKHYLNYRYRDRSKLLERSSSSLDSRRYADMKSSASTDEFSTSIAQKRSSDILEDIKHLEAKANDGSSDNGMLTKKSSYIWEDMKNLEARRQDTFSDSARNFSKHRLEVPDINITGESRKSYVVHPKINIDENSDSILKTMACGKSNGTDDGRESKLGVWTKVKPRKKSDNGRRNSDRALKIIQENSAILQKILACQAKKRLPDLEEISKEITISPINEEISKIFSPILEKMGLNEHEINEELARINFKDFDNMTATSVSEFDAKINEELSKLSLIDETEQMDHFDVDEVISHEYLNTREALIDRKINEELSKLLANYDDHSPASMVSLDKGPSSQNISEIEGLDLSSISTNVFSYKSSNDSIDTRSDLGSTQEPSILVDKFPKYTAYAVPKYHVDDSSPKSDIDIYRELEKLDKISSAQVLPDPTLELPQKELSSIPYVPNTSPFKDVSPLRYTSKPAQYDTSPLKTSYDPYKTFDFKPKLSPKQVSTNPFVSATYEKPVVDTAFEYINNKPEISINTYDLHLKSPMMTKESLEFRVRYDDDPVREVNTDYMSLQPESRSITTPSKSPYFSNGNTEPLTPTKYISKEERILDTGGTSFLNYPSESSDLLRRKYDALSPKEYTHTKSTTDYDRHLGTLPPIEPGTETGSYLPTRHRDYHSLSPNRQFPDHFPSTINHHYTSKLSSGLSDESKRPVSHPEFPGKMNVGKYAELPDRSSSSYKKSSLSLGNIGHSSKGADNNFNTVTSPKTQFSPFPVRNAPRKPKELTLKLGLYSPKGSDTGQLKRS

Target ORF information:

RefSeq Version	XM_003490922.3
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003490922.3

ORF Insert Sequence:

ATGAGCTGTA ACGTGATGCC TCTGAAGAAG TACGCGGAAA TTAGTTACCG CGTGAAATGT 
GTGGCCGTGG AAAATGAGCG CGTGTTTGGT GACGTGTACG TCACACAACA AACACGAAAT 
TTGCTGTATT GCGCAGATGG ATATCAGTTT CCCTCGTTGT ATAACAGAAG CAGACGCAAT 
CTGATTCAGA AATCATGGGA AGGTCCGGAT TTCATGGAAC TGAATCGGAA AAAGCCGATC 
AGAAGGGCTA GTTGCACGCC GCCCAAAGTG GAGAGAACAA ATTTGAAGAG GTCGAAATCT 
TTGGGTGACA CGTGGGACGT AATCGAAACT GATTGCAAAA ATGCTAACAA TTCGAAAGAT 
GAAAATTACA TTTGCAAGCC AAAGACATAT CCATCACCGT TACCGAATCC ACCCGAGAAC 
AAAAAAGGAA CTGTGTCTTC GTTAGTTGAT CAATTGTTGC TAGATATTTA TGGATTGCCA 
GACGATGACA GAAGACGGTC AGAAAGTGAC TCGACAGCCT CATCCCTGAA GATACGTCCG 
CAACATCAGC ACCTGCAGAA GGCGCGTTTA CTCTGGAAAA GTGAGCACGA GCTTCAAGTT 
TTAGTGCTGA ATTTACGCGA CCATATTAAT CACACTGGTG AAATACTCAT TCGACAGTTG 
CGTCGTAAAG ATCATCTCGC CACCAAATGT GAGAAACTAT GCGCCATAAT TACGGCTCAC 
CTTCTGGCAG TCAGCCCAAA ATGCGTCGAA GACACGAAGA TGAGATTTAG CCTGACGCCG 
CAACCAGGAG AAAGTGGTTT TATACAATGG CTGGACGCGA TGAAAATGGT TGCCAGGTTA 
CCAGGAGGAA TTCCGCCGGA ATTTCGAAAG AAGTTGTGGC TGACACTGGC AGAACGTCAT 
CTAGAGCAGC GCGGCGTAGA TTGGAAGCAG GCTGAAAAGA TCTGCTTCAA CGAGTGGAGC 
AATCCTGACG ATGAAGAGCT TGGTATACAA ATCGTTAAAG ACCTACACAG AACGGGCTGC 
AGTCTGTTTT GCGGTGCAGC TGGCAGAGAC AATCAAGCAG TGCTTCGTCG AGTTCTGCTT 
GGTTTCGCCC GATGGAACAA GTCCGTCGGC TATTGCCAGG GCCTGAACGT GTTGGCCGCC 
CTCGTTTTGC AAGTAATGGA TCGCGCCGAG TCCGCCGCGG TGAAGGTGAT GATCTACTTG 
ATAGAGGGTG TCCTGCCCGA GGGTTACTTC GCGGACAATC TCCGTGGCCT GTCGGTCGAC 
ATGGCGGTAT TTCGAGATCT CCTCCGGGGC AGACTGCCCA AGCTCTCGAA ACATCTGGAG 
GCTCTGCAGA ACGATGCTAA GGACAAGGCG ACAGGCAGCA GTTACGAGCC ACCTCTGACG 
AATGTGTTCA CGATGCAATG GTTCCTTACT CTTTTCTGTC ACTGTTTGCC ACAAGAGGCT 
GTCCTGCGCG TATGGGACCT CATATTTCTC GAAGGAGATG AGATATTGCT TAGAACCGCA 
CTGGCCATCT GGGAAGGACT TTCCGATCGT ATTATGACGG TGACATCGGC GGACGAGTTT 
TATAGTATAA TGGGAGTGCT GACAAGGGAG ATGCTCGAGT TCACGGATAC TAATAACCTC 
ATAAAGAATA TCGTTAGTAT GGGATCTTTG CATGGTGTAA CGGGGCTAAG AGAGAAACAT 
AGATACAATA TCACACCTTG GGCAAGAAAA TTGAGCGACG ACGAGGACAG CGACACGGAG 
GAGGATGAAA GGCTGGCCGT GGCTGCTGCG ATGTTCAGTA TGGCACAGCG ATTGAAGAAA 
GATCGTATAC CTCAAACGAT TGGAGCGCTG CAGGCAATGG CACCCAGCAG CGACCGAGAA 
CGGCTCGCTT TGGACATTAC CACTTTGAAA CAGCAGTACG CCAAGCTACG CGAACGTCAA 
CGACAAGCGC ATATTATACT TTCTGCTGCA TGTGCAAGGC AGACCATGGT ACCACCTCCT 
ACTTCTCAGG CCATGAATCA TCTTTTAGTG GGCAAAAGTG CGCTCGTGAG TGGGAAGAAT 
CGACCCCTGA GCCTACCTTC TGGTGCTGCA ACGACGAAGA TAAGACCTAC GACGCTAAAT 
CAAAACCGAA GGGACAGACA AGGCGTCACG CTTCACTGGA AAGACACCAA AAAACCGAAG 
CAGAAACCCT CGAATTTACC AGAAACGGTG GAATGTAGCG TTGTAACAAT GCAATCCACG 
GAAGCTGAGC TGGCTTCTCC AAAGCGGAGT AACGGTGACA GTGATAGCGA CAGCACATCG 
ACGGAATTGT GCGACGAACC GGACCGTCTC AGTGACGTTG ACAGCGAAGA TCTGACGTCA 
GCATCCGAAT CCTGTGTTGT CAGTACGGAT GAGGAGAAAA GCTCTCGAAT CACCGGATCA 
CCGATTCGCG AGAAGCCACT CGATCGTTCG TTTCTCGAAG AGAAGATGGA ACCAATTAAC 
GCCGAAGATT CGAAGGACTC GAAAAGCTCC GAGGATAACC TTGGTGACAT ATCAATCGCT 
AAAATCACCG ACCAAATAAG ACGATTATCA GCAGAGGATG ACAATAATTC CATGGTTCCT 
CAAAAATTTA GCGTGCAGAG CAAAGAATCG CCGGAAGAAC AATCGACGAA AGAATCGAAA 
AAAGAGAAAT CCATATCAGA TCTATCTATA GATTACACTT CCGATAGTAA TATCATTAAA 
AAATCGTTAT TGGACTTGAA CGAGTACGAT TATCTAGGTT TCAGTGGCAG TAAGATCAGT 
TCGAAAGAAG ATGAAGTATT AGTGCACGAA AGGCATCGAT CTAAACAGAC AACAAACGAG 
ATTGTAGATA TACCTCGAAA CGAAGTTGCG ACAATCAAGA AAGACCAAAC TGAAGACAAG 
ATAACTACGA CTATGAAACA GGATTCTTTA AACATATACT TGGATTATTC AGCATCCTTG 
GACAAGAAAT ACGATAGAAT CAAAGATAAA TCAAGCCTCG ATGTAGAGAT AGAAAATGAA 
ATTAGATCCG ACCGATATAA AACATCCTTC GATTTCTCTA GTAGAATATC TTTGGACTTG 
AAACCGTATG AAACTTCAAA AGTAGGAAGT TTAACCAGTG ACACTGCAGA CACAGTGAAT 
ACTGAAAGAA CGCTTTATGG CAAGGCCTAC GTTGGTCATC TTCCTATTTC ACCTGTAACA 
GTTGATCAAA AATTAAGCAG AGTGACACCC AGTATACATA CAACGCATAA TACTACTGTA 
TTAACTCCTG AAACGCCTGA AATTGTACAT AAAACGTTAG AGAACACGTC ATATAACGAT 
AGAATACAGA CGACTCAAAC GAAAATTTAT TCAGACCTAG TGAAGAGTCC TAAAACACCG 
GAAAGCAACA AATCATTCAG TTCAGGTGAA ACCATGGGAC CAGATTCGAA ACCATCGAGT 
CTGACAGTTA GCCCAAGGAC GCCGGTTAGA TCAGAATCTC GAGATTTCCC TATGGACAAA 
TCCCTGTGTT CGTCAGTTAG CTCAGACTCG AAGACTTTGG TTCTTCATTC TGCAGATTCA 
GACAGTACGA GGGACAGCAC GAAAACCGAC GCGAAGAAAA TGTATGAGAT TTCCAATTCG 
GCGACACCTA TCAGCATGGA CTCGTTGCAG AAATCAGAAG CCAGCCCAAA ACTAGTCGTG 
AGCAGCTCCA GTGAATCGTG TAGCCCGAGT AAATTAAAGT CTTCCGAGAG GTCGTTCAGT 
TCGGACCTGC AATCGCCAAT AAGTGCCAAC TCCATAAAGT ATACTTCGAA TTTCAGTAGA 
CGGGGCCAAG ATGACGTATC AGGCTCGCCA ATGGACTTGA GCAGCGCTAC CTCGCCGTTT 
TATCCGATCT CGAATCCGAA TCAAAAAACT CCGACGTCCC CGTACAGTGC TTCTAGAAGA 
AGATCCAGTT TGGACAGCAA CGAAACGTCA CCTGAGCAGA AATCGACCAG TTCGAGGGAG 
TCGAACAAGT TTCTAGGTTA TCAGTCGAAA TTTGACTCCT CCATAAAGAC GTCTGATATG 
AAAGATGTAG ACGGTATAGC ACGAAAAGGT GACACATTCG TCAGACCAGA GTTCATCACG 
AGGAATGAAG ATTCGAGTAT TAATTTGACG GATAGAAGAA TCAATGTTAG TGATGTTAAG 
TATTATCAAA CAACGAGTAC GGATTACTAT CGCAAGGATA AGGATATTTT TGATGACGGA 
GGGGATGATC CGTCATCGGA GAAAGACGTG GTACCTTCTT TGCCTCAGGA GAAGCTAGAT 
AAACATTATT TGAACTATCG ATATAGAGAT AGATCGAAGT TGTTGGAAAG AAGTTCAAGC 
AGTTTGGACA GTAGAAGGTA CGCTGATATG AAGTCATCGG CTTCCACCGA TGAATTTTCA 
ACGTCGATCG CGCAGAAGAG ATCCAGTGAC ATTTTAGAAG ATATTAAACA CTTGGAGGCG 
AAAGCAAACG ATGGGTCGTC GGATAATGGG ATGTTGACGA AGAAGAGCAG TTATATTTGG 
GAGGATATGA AGAATTTAGA AGCCAGGAGG CAAGATACCT TTAGTGATTC AGCGAGGAAC 
TTTTCGAAAC ATCGTCTGGA GGTACCGGAT ATAAATATAA CCGGAGAAAG CAGAAAATCG 
TACGTAGTAC ATCCGAAGAT CAATATCGAC GAAAATAGCG ACAGCATATT GAAGACCATG 
GCCTGTGGTA AGAGTAACGG CACAGATGAT GGCAGGGAGT CGAAGTTGGG CGTGTGGACG 
AAGGTGAAGC CTCGTAAAAA GAGTGACAAT GGGCGGAGGA ACAGCGACAG GGCGTTGAAG 
ATCATTCAAG AAAACTCCGC TATACTTCAG AAGATTCTTG CGTGTCAGGC AAAAAAGCGT 
CTGCCAGACC TGGAGGAGAT ATCGAAAGAA ATTACCATTA GCCCTATAAA CGAGGAGATT 
TCGAAGATCT TTAGTCCGAT ATTAGAGAAA ATGGGATTAA ACGAACACGA GATTAACGAG 
GAATTGGCGA GAATCAATTT CAAGGATTTC GACAACATGA CCGCTACCAG TGTGTCCGAG 
TTTGATGCAA AGATTAACGA GGAATTATCG AAGCTTTCTC TGATCGACGA GACGGAGCAG 
ATGGACCACT TCGACGTGGA CGAGGTGATA TCCCACGAAT ACTTGAACAC GAGAGAAGCT 
CTAATAGATC GTAAGATAAA CGAGGAATTG TCGAAATTAT TGGCAAACTA CGACGATCAT 
TCTCCGGCTA GTATGGTGAG TTTGGACAAA GGACCATCCA GCCAGAATAT TAGCGAGATC 
GAAGGACTGG ATCTGTCCAG TATTTCAACT AACGTATTTT CCTATAAATC ATCCAATGAC 
TCCATTGACA CGAGAAGCGA CTTAGGCTCG ACGCAGGAAC CATCCATTCT CGTTGACAAG 
TTTCCGAAAT ATACGGCATA CGCCGTACCG AAGTATCACG TGGACGACTC ATCACCTAAA 
AGCGACATAG ACATCTACAG AGAATTGGAG AAACTCGATA AGATCTCTTC CGCACAGGTG 
TTACCAGATC CGACTCTGGA GCTTCCACAG AAGGAATTGT CCTCGATTCC GTACGTTCCA 
AATACGTCGC CCTTCAAGGA TGTGTCACCA TTGAGGTACA CTTCCAAGCC GGCTCAATAC 
GACACTTCAC CCCTGAAGAC TTCTTACGAT CCCTACAAGA CCTTTGACTT CAAGCCCAAG 
TTGTCACCTA AACAAGTTTC CACTAATCCA TTTGTGTCAG CTACGTATGA GAAACCTGTG 
GTGGATACCG CTTTCGAGTA CATTAACAAT AAACCAGAGA TCTCGATCAA TACGTATGAT 
CTACATTTGA AGAGTCCAAT GATGACGAAG GAATCGCTAG AGTTTCGTGT CAGATACGAC 
GATGATCCAG TCAGAGAAGT TAACACCGAT TACATGTCTC TCCAGCCAGA AAGTAGATCG 
ATTACTACCC CGAGCAAGTC TCCTTATTTC AGCAATGGAA ATACCGAACC GTTAACGCCT 
ACGAAATATA TTTCTAAGGA GGAAAGAATC TTGGATACTG GTGGTACCTC GTTTCTGAAC 
TATCCCAGCG AATCTTCCGA CCTTCTTCGT CGAAAATACG ACGCTTTGTC TCCTAAGGAG 
TACACTCATA CCAAGAGTAC GACGGATTAT GATAGACACT TAGGCACGTT ACCTCCTATA 
GAACCTGGAA CAGAGACAGG ATCGTATTTA CCGACGAGAC ACCGAGATTA TCATTCTCTA 
TCACCGAATC GTCAGTTTCC TGATCACTTT CCTTCTACTA TCAATCATCA CTACACCTCG 
AAACTTTCAT CAGGTCTCTC TGACGAATCG AAACGACCGG TTTCTCATCC GGAATTCCCT 
GGCAAGATGA ATGTTGGGAA ATATGCAGAA TTACCCGATA GAAGTTCCAG CAGTTACAAG 
AAATCATCTC TTAGCTTAGG TAACATAGGA CACTCGAGTA AAGGAGCTGA TAATAATTTC 
AACACTGTGA CCAGTCCAAA AACGCAGTTC AGTCCCTTTC CCGTTAGAAA CGCTCCTAGG 
AAGCCGAAAG AACTTACGCT GAAATTGGGC CTCTATTCGC CCAAAGGTTC TGATACAGGA 
CAGTTAAAAA GGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100749006 ( XM_003490922.2 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_003490970.1 Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X1, mRNA.

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CloneID	OBo106112
Clone ID Related Accession (Same CDS sequence)	XM_003490922.3 , XM_003490922.2
Accession Version	XM_003490922.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	uncharacterized protein LOC100749006 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176906.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2015 this sequence version replaced XM_003490922.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTA ACGTGATGCC TCTGAAGAAG TACGCGGAAA TTAGTTACCG CGTGAAATGT 
GTGGCCGTGG AAAATGAGCG CGTGTTTGGT GACGTGTACG TCACACAACA AACACGAAAT 
TTGCTGTATT GCGCAGATGG ATATCAGTTT CCCTCGTTGT ATAACAGAAG CAGACGCAAT 
CTGATTCAGA AATCATGGGA AGGTCCGGAT TTCATGGAAC TGAATCGGAA AAAGCCGATC 
AGAAGGGCTA GTTGCACGCC GCCCAAAGTG GAGAGAACAA ATTTGAAGAG GTCGAAATCT 
TTGGGTGACA CGTGGGACGT AATCGAAACT GATTGCAAAA ATGCTAACAA TTCGAAAGAT 
GAAAATTACA TTTGCAAGCC AAAGACATAT CCATCACCGT TACCGAATCC ACCCGAGAAC 
AAAAAAGGAA CTGTGTCTTC GTTAGTTGAT CAATTGTTGC TAGATATTTA TGGATTGCCA 
GACGATGACA GAAGACGGTC AGAAAGTGAC TCGACAGCCT CATCCCTGAA GATACGTCCG 
CAACATCAGC ACCTGCAGAA GGCGCGTTTA CTCTGGAAAA GTGAGCACGA GCTTCAAGTT 
TTAGTGCTGA ATTTACGCGA CCATATTAAT CACACTGGTG AAATACTCAT TCGACAGTTG 
CGTCGTAAAG ATCATCTCGC CACCAAATGT GAGAAACTAT GCGCCATAAT TACGGCTCAC 
CTTCTGGCAG TCAGCCCAAA ATGCGTCGAA GACACGAAGA TGAGATTTAG CCTGACGCCG 
CAACCAGGAG AAAGTGGTTT TATACAATGG CTGGACGCGA TGAAAATGGT TGCCAGGTTA 
CCAGGAGGAA TTCCGCCGGA ATTTCGAAAG AAGTTGTGGC TGACACTGGC AGAACGTCAT 
CTAGAGCAGC GCGGCGTAGA TTGGAAGCAG GCTGAAAAGA TCTGCTTCAA CGAGTGGAGC 
AATCCTGACG ATGAAGAGCT TGGTATACAA ATCGTTAAAG ACCTACACAG AACGGGCTGC 
AGTCTGTTTT GCGGTGCAGC TGGCAGAGAC AATCAAGCAG TGCTTCGTCG AGTTCTGCTT 
GGTTTCGCCC GATGGAACAA GTCCGTCGGC TATTGCCAGG GCCTGAACGT GTTGGCCGCC 
CTCGTTTTGC AAGTAATGGA TCGCGCCGAG TCCGCCGCGG TGAAGGTGAT GATCTACTTG 
ATAGAGGGTG TCCTGCCCGA GGGTTACTTC GCGGACAATC TCCGTGGCCT GTCGGTCGAC 
ATGGCGGTAT TTCGAGATCT CCTCCGGGGC AGACTGCCCA AGCTCTCGAA ACATCTGGAG 
GCTCTGCAGA ACGATGCTAA GGACAAGGCG ACAGGCAGCA GTTACGAGCC ACCTCTGACG 
AATGTGTTCA CGATGCAATG GTTCCTTACT CTTTTCTGTC ACTGTTTGCC ACAAGAGGCT 
GTCCTGCGCG TATGGGACCT CATATTTCTC GAAGGAGATG AGATATTGCT TAGAACCGCA 
CTGGCCATCT GGGAAGGACT TTCCGATCGT ATTATGACGG TGACATCGGC GGACGAGTTT 
TATAGTATAA TGGGAGTGCT GACAAGGGAG ATGCTCGAGT TCACGGATAC TAATAACCTC 
ATAAAGAATA TCGTTAGTAT GGGATCTTTG CATGGTGTAA CGGGGCTAAG AGAGAAACAT 
AGATACAATA TCACACCTTG GGCAAGAAAA TTGAGCGACG ACGAGGACAG CGACACGGAG 
GAGGATGAAA GGCTGGCCGT GGCTGCTGCG ATGTTCAGTA TGGCACAGCG ATTGAAGAAA 
GATCGTATAC CTCAAACGAT TGGAGCGCTG CAGGCAATGG CACCCAGCAG CGACCGAGAA 
CGGCTCGCTT TGGACATTAC CACTTTGAAA CAGCAGTACG CCAAGCTACG CGAACGTCAA 
CGACAAGCGC ATATTATACT TTCTGCTGCA TGTGCAAGGC AGACCATGGT ACCACCTCCT 
ACTTCTCAGG CCATGAATCA TCTTTTAGTG GGCAAAAGTG CGCTCGTGAG TGGGAAGAAT 
CGACCCCTGA GCCTACCTTC TGGTGCTGCA ACGACGAAGA TAAGACCTAC GACGCTAAAT 
CAAAACCGAA GGGACAGACA AGGCGTCACG CTTCACTGGA AAGACACCAA AAAACCGAAG 
CAGAAACCCT CGAATTTACC AGAAACGGTG GAATGTAGCG TTGTAACAAT GCAATCCACG 
GAAGCTGAGC TGGCTTCTCC AAAGCGGAGT AACGGTGACA GTGATAGCGA CAGCACATCG 
ACGGAATTGT GCGACGAACC GGACCGTCTC AGTGACGTTG ACAGCGAAGA TCTGACGTCA 
GCATCCGAAT CCTGTGTTGT CAGTACGGAT GAGGAGAAAA GCTCTCGAAT CACCGGATCA 
CCGATTCGCG AGAAGCCACT CGATCGTTCG TTTCTCGAAG AGAAGATGGA ACCAATTAAC 
GCCGAAGATT CGAAGGACTC GAAAAGCTCC GAGGATAACC TTGGTGACAT ATCAATCGCT 
AAAATCACCG ACCAAATAAG ACGATTATCA GCAGAGGATG ACAATAATTC CATGGTTCCT 
CAAAAATTTA GCGTGCAGAG CAAAGAATCG CCGGAAGAAC AATCGACGAA AGAATCGAAA 
AAAGAGAAAT CCATATCAGA TCTATCTATA GATTACACTT CCGATAGTAA TATCATTAAA 
AAATCGTTAT TGGACTTGAA CGAGTACGAT TATCTAGGTT TCAGTGGCAG TAAGATCAGT 
TCGAAAGAAG ATGAAGTATT AGTGCACGAA AGGCATCGAT CTAAACAGAC AACAAACGAG 
ATTGTAGATA TACCTCGAAA CGAAGTTGCG ACAATCAAGA AAGACCAAAC TGAAGACAAG 
ATAACTACGA CTATGAAACA GGATTCTTTA AACATATACT TGGATTATTC AGCATCCTTG 
GACAAGAAAT ACGATAGAAT CAAAGATAAA TCAAGCCTCG ATGTAGAGAT AGAAAATGAA 
ATTAGATCCG ACCGATATAA AACATCCTTC GATTTCTCTA GTAGAATATC TTTGGACTTG 
AAACCGTATG AAACTTCAAA AGTAGGAAGT TTAACCAGTG ACACTGCAGA CACAGTGAAT 
ACTGAAAGAA CGCTTTATGG CAAGGCCTAC GTTGGTCATC TTCCTATTTC ACCTGTAACA 
GTTGATCAAA AATTAAGCAG AGTGACACCC AGTATACATA CAACGCATAA TACTACTGTA 
TTAACTCCTG AAACGCCTGA AATTGTACAT AAAACGTTAG AGAACACGTC ATATAACGAT 
AGAATACAGA CGACTCAAAC GAAAATTTAT TCAGACCTAG TGAAGAGTCC TAAAACACCG 
GAAAGCAACA AATCATTCAG TTCAGGTGAA ACCATGGGAC CAGATTCGAA ACCATCGAGT 
CTGACAGTTA GCCCAAGGAC GCCGGTTAGA TCAGAATCTC GAGATTTCCC TATGGACAAA 
TCCCTGTGTT CGTCAGTTAG CTCAGACTCG AAGACTTTGG TTCTTCATTC TGCAGATTCA 
GACAGTACGA GGGACAGCAC GAAAACCGAC GCGAAGAAAA TGTATGAGAT TTCCAATTCG 
GCGACACCTA TCAGCATGGA CTCGTTGCAG AAATCAGAAG CCAGCCCAAA ACTAGTCGTG 
AGCAGCTCCA GTGAATCGTG TAGCCCGAGT AAATTAAAGT CTTCCGAGAG GTCGTTCAGT 
TCGGACCTGC AATCGCCAAT AAGTGCCAAC TCCATAAAGT ATACTTCGAA TTTCAGTAGA 
CGGGGCCAAG ATGACGTATC AGGCTCGCCA ATGGACTTGA GCAGCGCTAC CTCGCCGTTT 
TATCCGATCT CGAATCCGAA TCAAAAAACT CCGACGTCCC CGTACAGTGC TTCTAGAAGA 
AGATCCAGTT TGGACAGCAA CGAAACGTCA CCTGAGCAGA AATCGACCAG TTCGAGGGAG 
TCGAACAAGT TTCTAGGTTA TCAGTCGAAA TTTGACTCCT CCATAAAGAC GTCTGATATG 
AAAGATGTAG ACGGTATAGC ACGAAAAGGT GACACATTCG TCAGACCAGA GTTCATCACG 
AGGAATGAAG ATTCGAGTAT TAATTTGACG GATAGAAGAA TCAATGTTAG TGATGTTAAG 
TATTATCAAA CAACGAGTAC GGATTACTAT CGCAAGGATA AGGATATTTT TGATGACGGA 
GGGGATGATC CGTCATCGGA GAAAGACGTG GTACCTTCTT TGCCTCAGGA GAAGCTAGAT 
AAACATTATT TGAACTATCG ATATAGAGAT AGATCGAAGT TGTTGGAAAG AAGTTCAAGC 
AGTTTGGACA GTAGAAGGTA CGCTGATATG AAGTCATCGG CTTCCACCGA TGAATTTTCA 
ACGTCGATCG CGCAGAAGAG ATCCAGTGAC ATTTTAGAAG ATATTAAACA CTTGGAGGCG 
AAAGCAAACG ATGGGTCGTC GGATAATGGG ATGTTGACGA AGAAGAGCAG TTATATTTGG 
GAGGATATGA AGAATTTAGA AGCCAGGAGG CAAGATACCT TTAGTGATTC AGCGAGGAAC 
TTTTCGAAAC ATCGTCTGGA GGTACCGGAT ATAAATATAA CCGGAGAAAG CAGAAAATCG 
TACGTAGTAC ATCCGAAGAT CAATATCGAC GAAAATAGCG ACAGCATATT GAAGACCATG 
GCCTGTGGTA AGAGTAACGG CACAGATGAT GGCAGGGAGT CGAAGTTGGG CGTGTGGACG 
AAGGTGAAGC CTCGTAAAAA GAGTGACAAT GGGCGGAGGA ACAGCGACAG GGCGTTGAAG 
ATCATTCAAG AAAACTCCGC TATACTTCAG AAGATTCTTG CGTGTCAGGC AAAAAAGCGT 
CTGCCAGACC TGGAGGAGAT ATCGAAAGAA ATTACCATTA GCCCTATAAA CGAGGAGATT 
TCGAAGATCT TTAGTCCGAT ATTAGAGAAA ATGGGATTAA ACGAACACGA GATTAACGAG 
GAATTGGCGA GAATCAATTT CAAGGATTTC GACAACATGA CCGCTACCAG TGTGTCCGAG 
TTTGATGCAA AGATTAACGA GGAATTATCG AAGCTTTCTC TGATCGACGA GACGGAGCAG 
ATGGACCACT TCGACGTGGA CGAGGTGATA TCCCACGAAT ACTTGAACAC GAGAGAAGCT 
CTAATAGATC GTAAGATAAA CGAGGAATTG TCGAAATTAT TGGCAAACTA CGACGATCAT 
TCTCCGGCTA GTATGGTGAG TTTGGACAAA GGACCATCCA GCCAGAATAT TAGCGAGATC 
GAAGGACTGG ATCTGTCCAG TATTTCAACT AACGTATTTT CCTATAAATC ATCCAATGAC 
TCCATTGACA CGAGAAGCGA CTTAGGCTCG ACGCAGGAAC CATCCATTCT CGTTGACAAG 
TTTCCGAAAT ATACGGCATA CGCCGTACCG AAGTATCACG TGGACGACTC ATCACCTAAA 
AGCGACATAG ACATCTACAG AGAATTGGAG AAACTCGATA AGATCTCTTC CGCACAGGTG 
TTACCAGATC CGACTCTGGA GCTTCCACAG AAGGAATTGT CCTCGATTCC GTACGTTCCA 
AATACGTCGC CCTTCAAGGA TGTGTCACCA TTGAGGTACA CTTCCAAGCC GGCTCAATAC 
GACACTTCAC CCCTGAAGAC TTCTTACGAT CCCTACAAGA CCTTTGACTT CAAGCCCAAG 
TTGTCACCTA AACAAGTTTC CACTAATCCA TTTGTGTCAG CTACGTATGA GAAACCTGTG 
GTGGATACCG CTTTCGAGTA CATTAACAAT AAACCAGAGA TCTCGATCAA TACGTATGAT 
CTACATTTGA AGAGTCCAAT GATGACGAAG GAATCGCTAG AGTTTCGTGT CAGATACGAC 
GATGATCCAG TCAGAGAAGT TAACACCGAT TACATGTCTC TCCAGCCAGA AAGTAGATCG 
ATTACTACCC CGAGCAAGTC TCCTTATTTC AGCAATGGAA ATACCGAACC GTTAACGCCT 
ACGAAATATA TTTCTAAGGA GGAAAGAATC TTGGATACTG GTGGTACCTC GTTTCTGAAC 
TATCCCAGCG AATCTTCCGA CCTTCTTCGT CGAAAATACG ACGCTTTGTC TCCTAAGGAG 
TACACTCATA CCAAGAGTAC GACGGATTAT GATAGACACT TAGGCACGTT ACCTCCTATA 
GAACCTGGAA CAGAGACAGG ATCGTATTTA CCGACGAGAC ACCGAGATTA TCATTCTCTA 
TCACCGAATC GTCAGTTTCC TGATCACTTT CCTTCTACTA TCAATCATCA CTACACCTCG 
AAACTTTCAT CAGGTCTCTC TGACGAATCG AAACGACCGG TTTCTCATCC GGAATTCCCT 
GGCAAGATGA ATGTTGGGAA ATATGCAGAA TTACCCGATA GAAGTTCCAG CAGTTACAAG 
AAATCATCTC TTAGCTTAGG TAACATAGGA CACTCGAGTA AAGGAGCTGA TAATAATTTC 
AACACTGTGA CCAGTCCAAA AACGCAGTTC AGTCCCTTTC CCGTTAGAAA CGCTCCTAGG 
AAGCCGAAAG AACTTACGCT GAAATTGGGC CTCTATTCGC CCAAAGGTTC TGATACAGGA 
CAGTTAAAAA GGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003490970.1
CDS	948..7565
Translation	MSCNVMPLKKYAEISYRVKCVAVENERVFGDVYVTQQTRNLLYCADGYQFPSLYNRSRRNLIQKSWEGPDFMELNRKKPIRRASCTPPKVERTNLKRSKSLGDTWDVIETDCKNANNSKDENYICKPKTYPSPLPNPPENKKGTVSSLVDQLLLDIYGLPDDDRRRSESDSTASSLKIRPQHQHLQKARLLWKSEHELQVLVLNLRDHINHTGEILIRQLRRKDHLATKCEKLCAIITAHLLAVSPKCVEDTKMRFSLTPQPGESGFIQWLDAMKMVARLPGGIPPEFRKKLWLTLAERHLEQRGVDWKQAEKICFNEWSNPDDEELGIQIVKDLHRTGCSLFCGAAGRDNQAVLRRVLLGFARWNKSVGYCQGLNVLAALVLQVMDRAESAAVKVMIYLIEGVLPEGYFADNLRGLSVDMAVFRDLLRGRLPKLSKHLEALQNDAKDKATGSSYEPPLTNVFTMQWFLTLFCHCLPQEAVLRVWDLIFLEGDEILLRTALAIWEGLSDRIMTVTSADEFYSIMGVLTREMLEFTDTNNLIKNIVSMGSLHGVTGLREKHRYNITPWARKLSDDEDSDTEEDERLAVAAAMFSMAQRLKKDRIPQTIGALQAMAPSSDRERLALDITTLKQQYAKLRERQRQAHIILSAACARQTMVPPPTSQAMNHLLVGKSALVSGKNRPLSLPSGAATTKIRPTTLNQNRRDRQGVTLHWKDTKKPKQKPSNLPETVECSVVTMQSTEAELASPKRSNGDSDSDSTSTELCDEPDRLSDVDSEDLTSASESCVVSTDEEKSSRITGSPIREKPLDRSFLEEKMEPINAEDSKDSKSSEDNLGDISIAKITDQIRRLSAEDDNNSMVPQKFSVQSKESPEEQSTKESKKEKSISDLSIDYTSDSNIIKKSLLDLNEYDYLGFSGSKISSKEDEVLVHERHRSKQTTNEIVDIPRNEVATIKKDQTEDKITTTMKQDSLNIYLDYSASLDKKYDRIKDKSSLDVEIENEIRSDRYKTSFDFSSRISLDLKPYETSKVGSLTSDTADTVNTERTLYGKAYVGHLPISPVTVDQKLSRVTPSIHTTHNTTVLTPETPEIVHKTLENTSYNDRIQTTQTKIYSDLVKSPKTPESNKSFSSGETMGPDSKPSSLTVSPRTPVRSESRDFPMDKSLCSSVSSDSKTLVLHSADSDSTRDSTKTDAKKMYEISNSATPISMDSLQKSEASPKLVVSSSSESCSPSKLKSSERSFSSDLQSPISANSIKYTSNFSRRGQDDVSGSPMDLSSATSPFYPISNPNQKTPTSPYSASRRRSSLDSNETSPEQKSTSSRESNKFLGYQSKFDSSIKTSDMKDVDGIARKGDTFVRPEFITRNEDSSINLTDRRINVSDVKYYQTTSTDYYRKDKDIFDDGGDDPSSEKDVVPSLPQEKLDKHYLNYRYRDRSKLLERSSSSLDSRRYADMKSSASTDEFSTSIAQKRSSDILEDIKHLEAKANDGSSDNGMLTKKSSYIWEDMKNLEARRQDTFSDSARNFSKHRLEVPDINITGESRKSYVVHPKINIDENSDSILKTMACGKSNGTDDGRESKLGVWTKVKPRKKSDNGRRNSDRALKIIQENSAILQKILACQAKKRLPDLEEISKEITISPINEEISKIFSPILEKMGLNEHEINEELARINFKDFDNMTATSVSEFDAKINEELSKLSLIDETEQMDHFDVDEVISHEYLNTREALIDRKINEELSKLLANYDDHSPASMVSLDKGPSSQNISEIEGLDLSSISTNVFSYKSSNDSIDTRSDLGSTQEPSILVDKFPKYTAYAVPKYHVDDSSPKSDIDIYRELEKLDKISSAQVLPDPTLELPQKELSSIPYVPNTSPFKDVSPLRYTSKPAQYDTSPLKTSYDPYKTFDFKPKLSPKQVSTNPFVSATYEKPVVDTAFEYINNKPEISINTYDLHLKSPMMTKESLEFRVRYDDDPVREVNTDYMSLQPESRSITTPSKSPYFSNGNTEPLTPTKYISKEERILDTGGTSFLNYPSESSDLLRRKYDALSPKEYTHTKSTTDYDRHLGTLPPIEPGTETGSYLPTRHRDYHSLSPNRQFPDHFPSTINHHYTSKLSSGLSDESKRPVSHPEFPGKMNVGKYAELPDRSSSSYKKSSLSLGNIGHSSKGADNNFNTVTSPKTQFSPFPVRNAPRKPKELTLKLGLYSPKGSDTGQLKRS

Target ORF information:

RefSeq Version	XM_003490922.2
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003490922.2

ORF Insert Sequence:

ATGAGCTGTA ACGTGATGCC TCTGAAGAAG TACGCGGAAA TTAGTTACCG CGTGAAATGT 
GTGGCCGTGG AAAATGAGCG CGTGTTTGGT GACGTGTACG TCACACAACA AACACGAAAT 
TTGCTGTATT GCGCAGATGG ATATCAGTTT CCCTCGTTGT ATAACAGAAG CAGACGCAAT 
CTGATTCAGA AATCATGGGA AGGTCCGGAT TTCATGGAAC TGAATCGGAA AAAGCCGATC 
AGAAGGGCTA GTTGCACGCC GCCCAAAGTG GAGAGAACAA ATTTGAAGAG GTCGAAATCT 
TTGGGTGACA CGTGGGACGT AATCGAAACT GATTGCAAAA ATGCTAACAA TTCGAAAGAT 
GAAAATTACA TTTGCAAGCC AAAGACATAT CCATCACCGT TACCGAATCC ACCCGAGAAC 
AAAAAAGGAA CTGTGTCTTC GTTAGTTGAT CAATTGTTGC TAGATATTTA TGGATTGCCA 
GACGATGACA GAAGACGGTC AGAAAGTGAC TCGACAGCCT CATCCCTGAA GATACGTCCG 
CAACATCAGC ACCTGCAGAA GGCGCGTTTA CTCTGGAAAA GTGAGCACGA GCTTCAAGTT 
TTAGTGCTGA ATTTACGCGA CCATATTAAT CACACTGGTG AAATACTCAT TCGACAGTTG 
CGTCGTAAAG ATCATCTCGC CACCAAATGT GAGAAACTAT GCGCCATAAT TACGGCTCAC 
CTTCTGGCAG TCAGCCCAAA ATGCGTCGAA GACACGAAGA TGAGATTTAG CCTGACGCCG 
CAACCAGGAG AAAGTGGTTT TATACAATGG CTGGACGCGA TGAAAATGGT TGCCAGGTTA 
CCAGGAGGAA TTCCGCCGGA ATTTCGAAAG AAGTTGTGGC TGACACTGGC AGAACGTCAT 
CTAGAGCAGC GCGGCGTAGA TTGGAAGCAG GCTGAAAAGA TCTGCTTCAA CGAGTGGAGC 
AATCCTGACG ATGAAGAGCT TGGTATACAA ATCGTTAAAG ACCTACACAG AACGGGCTGC 
AGTCTGTTTT GCGGTGCAGC TGGCAGAGAC AATCAAGCAG TGCTTCGTCG AGTTCTGCTT 
GGTTTCGCCC GATGGAACAA GTCCGTCGGC TATTGCCAGG GCCTGAACGT GTTGGCCGCC 
CTCGTTTTGC AAGTAATGGA TCGCGCCGAG TCCGCCGCGG TGAAGGTGAT GATCTACTTG 
ATAGAGGGTG TCCTGCCCGA GGGTTACTTC GCGGACAATC TCCGTGGCCT GTCGGTCGAC 
ATGGCGGTAT TTCGAGATCT CCTCCGGGGC AGACTGCCCA AGCTCTCGAA ACATCTGGAG 
GCTCTGCAGA ACGATGCTAA GGACAAGGCG ACAGGCAGCA GTTACGAGCC ACCTCTGACG 
AATGTGTTCA CGATGCAATG GTTCCTTACT CTTTTCTGTC ACTGTTTGCC ACAAGAGGCT 
GTCCTGCGCG TATGGGACCT CATATTTCTC GAAGGAGATG AGATATTGCT TAGAACCGCA 
CTGGCCATCT GGGAAGGACT TTCCGATCGT ATTATGACGG TGACATCGGC GGACGAGTTT 
TATAGTATAA TGGGAGTGCT GACAAGGGAG ATGCTCGAGT TCACGGATAC TAATAACCTC 
ATAAAGAATA TCGTTAGTAT GGGATCTTTG CATGGTGTAA CGGGGCTAAG AGAGAAACAT 
AGATACAATA TCACACCTTG GGCAAGAAAA TTGAGCGACG ACGAGGACAG CGACACGGAG 
GAGGATGAAA GGCTGGCCGT GGCTGCTGCG ATGTTCAGTA TGGCACAGCG ATTGAAGAAA 
GATCGTATAC CTCAAACGAT TGGAGCGCTG CAGGCAATGG CACCCAGCAG CGACCGAGAA 
CGGCTCGCTT TGGACATTAC CACTTTGAAA CAGCAGTACG CCAAGCTACG CGAACGTCAA 
CGACAAGCGC ATATTATACT TTCTGCTGCA TGTGCAAGGC AGACCATGGT ACCACCTCCT 
ACTTCTCAGG CCATGAATCA TCTTTTAGTG GGCAAAAGTG CGCTCGTGAG TGGGAAGAAT 
CGACCCCTGA GCCTACCTTC TGGTGCTGCA ACGACGAAGA TAAGACCTAC GACGCTAAAT 
CAAAACCGAA GGGACAGACA AGGCGTCACG CTTCACTGGA AAGACACCAA AAAACCGAAG 
CAGAAACCCT CGAATTTACC AGAAACGGTG GAATGTAGCG TTGTAACAAT GCAATCCACG 
GAAGCTGAGC TGGCTTCTCC AAAGCGGAGT AACGGTGACA GTGATAGCGA CAGCACATCG 
ACGGAATTGT GCGACGAACC GGACCGTCTC AGTGACGTTG ACAGCGAAGA TCTGACGTCA 
GCATCCGAAT CCTGTGTTGT CAGTACGGAT GAGGAGAAAA GCTCTCGAAT CACCGGATCA 
CCGATTCGCG AGAAGCCACT CGATCGTTCG TTTCTCGAAG AGAAGATGGA ACCAATTAAC 
GCCGAAGATT CGAAGGACTC GAAAAGCTCC GAGGATAACC TTGGTGACAT ATCAATCGCT 
AAAATCACCG ACCAAATAAG ACGATTATCA GCAGAGGATG ACAATAATTC CATGGTTCCT 
CAAAAATTTA GCGTGCAGAG CAAAGAATCG CCGGAAGAAC AATCGACGAA AGAATCGAAA 
AAAGAGAAAT CCATATCAGA TCTATCTATA GATTACACTT CCGATAGTAA TATCATTAAA 
AAATCGTTAT TGGACTTGAA CGAGTACGAT TATCTAGGTT TCAGTGGCAG TAAGATCAGT 
TCGAAAGAAG ATGAAGTATT AGTGCACGAA AGGCATCGAT CTAAACAGAC AACAAACGAG 
ATTGTAGATA TACCTCGAAA CGAAGTTGCG ACAATCAAGA AAGACCAAAC TGAAGACAAG 
ATAACTACGA CTATGAAACA GGATTCTTTA AACATATACT TGGATTATTC AGCATCCTTG 
GACAAGAAAT ACGATAGAAT CAAAGATAAA TCAAGCCTCG ATGTAGAGAT AGAAAATGAA 
ATTAGATCCG ACCGATATAA AACATCCTTC GATTTCTCTA GTAGAATATC TTTGGACTTG 
AAACCGTATG AAACTTCAAA AGTAGGAAGT TTAACCAGTG ACACTGCAGA CACAGTGAAT 
ACTGAAAGAA CGCTTTATGG CAAGGCCTAC GTTGGTCATC TTCCTATTTC ACCTGTAACA 
GTTGATCAAA AATTAAGCAG AGTGACACCC AGTATACATA CAACGCATAA TACTACTGTA 
TTAACTCCTG AAACGCCTGA AATTGTACAT AAAACGTTAG AGAACACGTC ATATAACGAT 
AGAATACAGA CGACTCAAAC GAAAATTTAT TCAGACCTAG TGAAGAGTCC TAAAACACCG 
GAAAGCAACA AATCATTCAG TTCAGGTGAA ACCATGGGAC CAGATTCGAA ACCATCGAGT 
CTGACAGTTA GCCCAAGGAC GCCGGTTAGA TCAGAATCTC GAGATTTCCC TATGGACAAA 
TCCCTGTGTT CGTCAGTTAG CTCAGACTCG AAGACTTTGG TTCTTCATTC TGCAGATTCA 
GACAGTACGA GGGACAGCAC GAAAACCGAC GCGAAGAAAA TGTATGAGAT TTCCAATTCG 
GCGACACCTA TCAGCATGGA CTCGTTGCAG AAATCAGAAG CCAGCCCAAA ACTAGTCGTG 
AGCAGCTCCA GTGAATCGTG TAGCCCGAGT AAATTAAAGT CTTCCGAGAG GTCGTTCAGT 
TCGGACCTGC AATCGCCAAT AAGTGCCAAC TCCATAAAGT ATACTTCGAA TTTCAGTAGA 
CGGGGCCAAG ATGACGTATC AGGCTCGCCA ATGGACTTGA GCAGCGCTAC CTCGCCGTTT 
TATCCGATCT CGAATCCGAA TCAAAAAACT CCGACGTCCC CGTACAGTGC TTCTAGAAGA 
AGATCCAGTT TGGACAGCAA CGAAACGTCA CCTGAGCAGA AATCGACCAG TTCGAGGGAG 
TCGAACAAGT TTCTAGGTTA TCAGTCGAAA TTTGACTCCT CCATAAAGAC GTCTGATATG 
AAAGATGTAG ACGGTATAGC ACGAAAAGGT GACACATTCG TCAGACCAGA GTTCATCACG 
AGGAATGAAG ATTCGAGTAT TAATTTGACG GATAGAAGAA TCAATGTTAG TGATGTTAAG 
TATTATCAAA CAACGAGTAC GGATTACTAT CGCAAGGATA AGGATATTTT TGATGACGGA 
GGGGATGATC CGTCATCGGA GAAAGACGTG GTACCTTCTT TGCCTCAGGA GAAGCTAGAT 
AAACATTATT TGAACTATCG ATATAGAGAT AGATCGAAGT TGTTGGAAAG AAGTTCAAGC 
AGTTTGGACA GTAGAAGGTA CGCTGATATG AAGTCATCGG CTTCCACCGA TGAATTTTCA 
ACGTCGATCG CGCAGAAGAG ATCCAGTGAC ATTTTAGAAG ATATTAAACA CTTGGAGGCG 
AAAGCAAACG ATGGGTCGTC GGATAATGGG ATGTTGACGA AGAAGAGCAG TTATATTTGG 
GAGGATATGA AGAATTTAGA AGCCAGGAGG CAAGATACCT TTAGTGATTC AGCGAGGAAC 
TTTTCGAAAC ATCGTCTGGA GGTACCGGAT ATAAATATAA CCGGAGAAAG CAGAAAATCG 
TACGTAGTAC ATCCGAAGAT CAATATCGAC GAAAATAGCG ACAGCATATT GAAGACCATG 
GCCTGTGGTA AGAGTAACGG CACAGATGAT GGCAGGGAGT CGAAGTTGGG CGTGTGGACG 
AAGGTGAAGC CTCGTAAAAA GAGTGACAAT GGGCGGAGGA ACAGCGACAG GGCGTTGAAG 
ATCATTCAAG AAAACTCCGC TATACTTCAG AAGATTCTTG CGTGTCAGGC AAAAAAGCGT 
CTGCCAGACC TGGAGGAGAT ATCGAAAGAA ATTACCATTA GCCCTATAAA CGAGGAGATT 
TCGAAGATCT TTAGTCCGAT ATTAGAGAAA ATGGGATTAA ACGAACACGA GATTAACGAG 
GAATTGGCGA GAATCAATTT CAAGGATTTC GACAACATGA CCGCTACCAG TGTGTCCGAG 
TTTGATGCAA AGATTAACGA GGAATTATCG AAGCTTTCTC TGATCGACGA GACGGAGCAG 
ATGGACCACT TCGACGTGGA CGAGGTGATA TCCCACGAAT ACTTGAACAC GAGAGAAGCT 
CTAATAGATC GTAAGATAAA CGAGGAATTG TCGAAATTAT TGGCAAACTA CGACGATCAT 
TCTCCGGCTA GTATGGTGAG TTTGGACAAA GGACCATCCA GCCAGAATAT TAGCGAGATC 
GAAGGACTGG ATCTGTCCAG TATTTCAACT AACGTATTTT CCTATAAATC ATCCAATGAC 
TCCATTGACA CGAGAAGCGA CTTAGGCTCG ACGCAGGAAC CATCCATTCT CGTTGACAAG 
TTTCCGAAAT ATACGGCATA CGCCGTACCG AAGTATCACG TGGACGACTC ATCACCTAAA 
AGCGACATAG ACATCTACAG AGAATTGGAG AAACTCGATA AGATCTCTTC CGCACAGGTG 
TTACCAGATC CGACTCTGGA GCTTCCACAG AAGGAATTGT CCTCGATTCC GTACGTTCCA 
AATACGTCGC CCTTCAAGGA TGTGTCACCA TTGAGGTACA CTTCCAAGCC GGCTCAATAC 
GACACTTCAC CCCTGAAGAC TTCTTACGAT CCCTACAAGA CCTTTGACTT CAAGCCCAAG 
TTGTCACCTA AACAAGTTTC CACTAATCCA TTTGTGTCAG CTACGTATGA GAAACCTGTG 
GTGGATACCG CTTTCGAGTA CATTAACAAT AAACCAGAGA TCTCGATCAA TACGTATGAT 
CTACATTTGA AGAGTCCAAT GATGACGAAG GAATCGCTAG AGTTTCGTGT CAGATACGAC 
GATGATCCAG TCAGAGAAGT TAACACCGAT TACATGTCTC TCCAGCCAGA AAGTAGATCG 
ATTACTACCC CGAGCAAGTC TCCTTATTTC AGCAATGGAA ATACCGAACC GTTAACGCCT 
ACGAAATATA TTTCTAAGGA GGAAAGAATC TTGGATACTG GTGGTACCTC GTTTCTGAAC 
TATCCCAGCG AATCTTCCGA CCTTCTTCGT CGAAAATACG ACGCTTTGTC TCCTAAGGAG 
TACACTCATA CCAAGAGTAC GACGGATTAT GATAGACACT TAGGCACGTT ACCTCCTATA 
GAACCTGGAA CAGAGACAGG ATCGTATTTA CCGACGAGAC ACCGAGATTA TCATTCTCTA 
TCACCGAATC GTCAGTTTCC TGATCACTTT CCTTCTACTA TCAATCATCA CTACACCTCG 
AAACTTTCAT CAGGTCTCTC TGACGAATCG AAACGACCGG TTTCTCATCC GGAATTCCCT 
GGCAAGATGA ATGTTGGGAA ATATGCAGAA TTACCCGATA GAAGTTCCAG CAGTTACAAG 
AAATCATCTC TTAGCTTAGG TAACATAGGA CACTCGAGTA AAGGAGCTGA TAATAATTTC 
AACACTGTGA CCAGTCCAAA AACGCAGTTC AGTCCCTTTC CCGTTAGAAA CGCTCCTAGG 
AAGCCGAAAG AACTTACGCT GAAATTGGGC CTCTATTCGC CCAAAGGTTC TGATACAGGA 
CAGTTAAAAA GGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100749006 ( XM_024368865.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_024224633.1 Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X2, mRNA.

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CloneID	OBo115590
Clone ID Related Accession (Same CDS sequence)	XM_024368865.1
Accession Version	XM_024368865.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	uncharacterized protein LOC100749006 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176906.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGTA ACGTGATGCC TCTGAAGAAG TACGCGGAAA TTAGTTACCG CGTGAAATGT 
GTGGCCGTGG AAAATGAGCG CGTGTTTGGT GACGTGTACG TCACACAACA AACACGAAAT 
TTGCTGTATT GCGCAGATGG ATATCAGTTT CCCTCGTTGT ATAACAGAAG CAGACGCAAT 
CTGATTCAGA AATCATGGGA AGGTCCGGAT TTCATGGAAC TGAATCGGAA AAAGCCGATC 
AGAAGGGCTA GTTGCACGCC GCCCAAAGTG GAGAGAACAA ATTTGAAGAG GTCGAAATCT 
TTGGGTGACA CGTGGGACGT AATCGAAACT GATTGCAAAA ATGCTAACAA TTCGAAAGAT 
GAAAATTACA TTTGCAAGCC AAAGACATAT CCATCACCGT TACCGAATCC ACCCGAGAAC 
AAAAAAGGAA CTGTGTCTTC GTTAGTTGAT CAATTGTTGC TAGATATTTA TGGATTGCCA 
GACGATGACA GAAGACGGTC AGAAAGTGAC TCGACAGCCT CATCCCTGAA GATACGTCCG 
CAACATCAGC ACCTGCAGAA GGCGCGTTTA CTCTGGAAAA GTGAGCACGA GCTTCAAGTT 
TTAGTGCTGA ATTTACGCGA CCATATTAAT CACACTGGTG AAATACTCAT TCGACAGTTG 
CGTCGTAAAG ATCATCTCGC CACCAAATGT GAGAAACTAT GCGCCATAAT TACGGCTCAC 
CTTCTGGCAG TCAGCCCAAA ATGCGTCGAA GACACGAAGA TGAGATTTAG CCTGACGCCG 
CAACCAGGAG AAAGTGGTTT TATACAATGG CTGGACGCGA TGAAAATGGT TGCCAGGTTA 
CCAGGAGGAA TTCCGCCGGA ATTTCGAAAG AAGTTGTGGC TGACACTGGC AGAACGTCAT 
CTAGAGCAGC GCGGCGTAGA TTGGAAGCAG GCTGAAAAGA TCTGCTTCAA CGAGTGGAGC 
AATCCTGACG ATGAAGAGCT TGGTATACAA ATCGTTAAAG ACCTACACAG AACGGGCTGC 
AGTCTGTTTT GCGGTGCAGC TGGCAGAGAC AATCAAGCAG TGCTTCGTCG AGTTCTGCTT 
GGTTTCGCCC GATGGAACAA GTCCGTCGGC TATTGCCAGG GCCTGAACGT GTTGGCCGCC 
CTCGTTTTGC AAGTAATGGA TCGCGCCGAG TCCGCCGCGG TGAAGGTGAT GATCTACTTG 
ATAGAGGGTG TCCTGCCCGA GGGTTACTTC GCGGACAATC TCCGTGGCCT GTCGGTCGAC 
ATGGCGGTAT TTCGAGATCT CCTCCGGGGC AGACTGCCCA AGCTCTCGAA ACATCTGGAG 
GCTCTGCAGA ACGATGCTAA GGACAAGGCG ACAGGCAGCA GTTACGAGCC ACCTCTGACG 
AATGTGTTCA CGATGCAATG GTTCCTTACT CTTTTCTGTC ACTGTTTGCC ACAAGAGGCT 
GTCCTGCGCG TATGGGACCT CATATTTCTC GAAGGAGATG AGATATTGCT TAGAACCGCA 
CTGGCCATCT GGGAAGGACT TTCCGATCGT ATTATGACGG TGACATCGGC GGACGAGTTT 
TATAGTATAA TGGGAGTGCT GACAAGGGAG ATGCTCGAGT TCACGGATAC TAATAACCTC 
ATAAAGAATA TCGTTAGTAT GGGATCTTTG CATGGTGTAA CGGGGCTAAG AGAGAAACAT 
AGATACAATA TCACACCTTG GGCAAGAAAA TTGAGCGACG ACGAGGACAG CGACACGGAG 
GAGGATGAAA GGCTGGCCGT GGCTGCTGCG ATGTTCAGTA TGGCACAGCG ATTGAAGAAA 
GATCGTATAC CTCAAACGAT TGGAGCGCTG CAGGCAATGG CACCCAGCAG CGACCGAGAA 
CGGCTCGCTT TGGACATTAC CACTTTGAAA CAGCAGTACG CCAAGCTACG CGAACGTCAA 
CGACAAGCGC ATATTATACT TTCTGTGGGC AAAAGTGCGC TCGTGAGTGG GAAGAATCGA 
CCCCTGAGCC TACCTTCTGG TGCTGCAACG ACGAAGATAA GACCTACGAC GCTAAATCAA 
AACCGAAGGG ACAGACAAGG CGTCACGCTT CACTGGAAAG ACACCAAAAA ACCGAAGCAG 
AAACCCTCGA ATTTACCAGA AACGGTGGAA TGTAGCGTTG TAACAATGCA ATCCACGGAA 
GCTGAGCTGG CTTCTCCAAA GCGGAGTAAC GGTGACAGTG ATAGCGACAG CACATCGACG 
GAATTGTGCG ACGAACCGGA CCGTCTCAGT GACGTTGACA GCGAAGATCT GACGTCAGCA 
TCCGAATCCT GTGTTGTCAG TACGGATGAG GAGAAAAGCT CTCGAATCAC CGGATCACCG 
ATTCGCGAGA AGCCACTCGA TCGTTCGTTT CTCGAAGAGA AGATGGAACC AATTAACGCC 
GAAGATTCGA AGGACTCGAA AAGCTCCGAG GATAACCTTG GTGACATATC AATCGCTAAA 
ATCACCGACC AAATAAGACG ATTATCAGCA GAGGATGACA ATAATTCCAT GGTTCCTCAA 
AAATTTAGCG TGCAGAGCAA AGAATCGCCG GAAGAACAAT CGACGAAAGA ATCGAAAAAA 
GAGAAATCCA TATCAGATCT ATCTATAGAT TACACTTCCG ATAGTAATAT CATTAAAAAA 
TCGTTATTGG ACTTGAACGA GTACGATTAT CTAGGTTTCA GTGGCAGTAA GATCAGTTCG 
AAAGAAGATG AAGTATTAGT GCACGAAAGG CATCGATCTA AACAGACAAC AAACGAGATT 
GTAGATATAC CTCGAAACGA AGTTGCGACA ATCAAGAAAG ACCAAACTGA AGACAAGATA 
ACTACGACTA TGAAACAGGA TTCTTTAAAC ATATACTTGG ATTATTCAGC ATCCTTGGAC 
AAGAAATACG ATAGAATCAA AGATAAATCA AGCCTCGATG TAGAGATAGA AAATGAAATT 
AGATCCGACC GATATAAAAC ATCCTTCGAT TTCTCTAGTA GAATATCTTT GGACTTGAAA 
CCGTATGAAA CTTCAAAAGT AGGAAGTTTA ACCAGTGACA CTGCAGACAC AGTGAATACT 
GAAAGAACGC TTTATGGCAA GGCCTACGTT GGTCATCTTC CTATTTCACC TGTAACAGTT 
GATCAAAAAT TAAGCAGAGT GACACCCAGT ATACATACAA CGCATAATAC TACTGTATTA 
ACTCCTGAAA CGCCTGAAAT TGTACATAAA ACGTTAGAGA ACACGTCATA TAACGATAGA 
ATACAGACGA CTCAAACGAA AATTTATTCA GACCTAGTGA AGAGTCCTAA AACACCGGAA 
AGCAACAAAT CATTCAGTTC AGGTGAAACC ATGGGACCAG ATTCGAAACC ATCGAGTCTG 
ACAGTTAGCC CAAGGACGCC GGTTAGATCA GAATCTCGAG ATTTCCCTAT GGACAAATCC 
CTGTGTTCGT CAGTTAGCTC AGACTCGAAG ACTTTGGTTC TTCATTCTGC AGATTCAGAC 
AGTACGAGGG ACAGCACGAA AACCGACGCG AAGAAAATGT ATGAGATTTC CAATTCGGCG 
ACACCTATCA GCATGGACTC GTTGCAGAAA TCAGAAGCCA GCCCAAAACT AGTCGTGAGC 
AGCTCCAGTG AATCGTGTAG CCCGAGTAAA TTAAAGTCTT CCGAGAGGTC GTTCAGTTCG 
GACCTGCAAT CGCCAATAAG TGCCAACTCC ATAAAGTATA CTTCGAATTT CAGTAGACGG 
GGCCAAGATG ACGTATCAGG CTCGCCAATG GACTTGAGCA GCGCTACCTC GCCGTTTTAT 
CCGATCTCGA ATCCGAATCA AAAAACTCCG ACGTCCCCGT ACAGTGCTTC TAGAAGAAGA 
TCCAGTTTGG ACAGCAACGA AACGTCACCT GAGCAGAAAT CGACCAGTTC GAGGGAGTCG 
AACAAGTTTC TAGGTTATCA GTCGAAATTT GACTCCTCCA TAAAGACGTC TGATATGAAA 
GATGTAGACG GTATAGCACG AAAAGGTGAC ACATTCGTCA GACCAGAGTT CATCACGAGG 
AATGAAGATT CGAGTATTAA TTTGACGGAT AGAAGAATCA ATGTTAGTGA TGTTAAGTAT 
TATCAAACAA CGAGTACGGA TTACTATCGC AAGGATAAGG ATATTTTTGA TGACGGAGGG 
GATGATCCGT CATCGGAGAA AGACGTGGTA CCTTCTTTGC CTCAGGAGAA GCTAGATAAA 
CATTATTTGA ACTATCGATA TAGAGATAGA TCGAAGTTGT TGGAAAGAAG TTCAAGCAGT 
TTGGACAGTA GAAGGTACGC TGATATGAAG TCATCGGCTT CCACCGATGA ATTTTCAACG 
TCGATCGCGC AGAAGAGATC CAGTGACATT TTAGAAGATA TTAAACACTT GGAGGCGAAA 
GCAAACGATG GGTCGTCGGA TAATGGGATG TTGACGAAGA AGAGCAGTTA TATTTGGGAG 
GATATGAAGA ATTTAGAAGC CAGGAGGCAA GATACCTTTA GTGATTCAGC GAGGAACTTT 
TCGAAACATC GTCTGGAGGT ACCGGATATA AATATAACCG GAGAAAGCAG AAAATCGTAC 
GTAGTACATC CGAAGATCAA TATCGACGAA AATAGCGACA GCATATTGAA GACCATGGCC 
TGTGGTAAGA GTAACGGCAC AGATGATGGC AGGGAGTCGA AGTTGGGCGT GTGGACGAAG 
GTGAAGCCTC GTAAAAAGAG TGACAATGGG CGGAGGAACA GCGACAGGGC GTTGAAGATC 
ATTCAAGAAA ACTCCGCTAT ACTTCAGAAG ATTCTTGCGT GTCAGGCAAA AAAGCGTCTG 
CCAGACCTGG AGGAGATATC GAAAGAAATT ACCATTAGCC CTATAAACGA GGAGATTTCG 
AAGATCTTTA GTCCGATATT AGAGAAAATG GGATTAAACG AACACGAGAT TAACGAGGAA 
TTGGCGAGAA TCAATTTCAA GGATTTCGAC AACATGACCG CTACCAGTGT GTCCGAGTTT 
GATGCAAAGA TTAACGAGGA ATTATCGAAG CTTTCTCTGA TCGACGAGAC GGAGCAGATG 
GACCACTTCG ACGTGGACGA GGTGATATCC CACGAATACT TGAACACGAG AGAAGCTCTA 
ATAGATCGTA AGATAAACGA GGAATTGTCG AAATTATTGG CAAACTACGA CGATCATTCT 
CCGGCTAGTA TGGTGAGTTT GGACAAAGGA CCATCCAGCC AGAATATTAG CGAGATCGAA 
GGACTGGATC TGTCCAGTAT TTCAACTAAC GTATTTTCCT ATAAATCATC CAATGACTCC 
ATTGACACGA GAAGCGACTT AGGCTCGACG CAGGAACCAT CCATTCTCGT TGACAAGTTT 
CCGAAATATA CGGCATACGC CGTACCGAAG TATCACGTGG ACGACTCATC ACCTAAAAGC 
GACATAGACA TCTACAGAGA ATTGGAGAAA CTCGATAAGA TCTCTTCCGC ACAGGTGTTA 
CCAGATCCGA CTCTGGAGCT TCCACAGAAG GAATTGTCCT CGATTCCGTA CGTTCCAAAT 
ACGTCGCCCT TCAAGGATGT GTCACCATTG AGGTACACTT CCAAGCCGGC TCAATACGAC 
ACTTCACCCC TGAAGACTTC TTACGATCCC TACAAGACCT TTGACTTCAA GCCCAAGTTG 
TCACCTAAAC AAGTTTCCAC TAATCCATTT GTGTCAGCTA CGTATGAGAA ACCTGTGGTG 
GATACCGCTT TCGAGTACAT TAACAATAAA CCAGAGATCT CGATCAATAC GTATGATCTA 
CATTTGAAGA GTCCAATGAT GACGAAGGAA TCGCTAGAGT TTCGTGTCAG ATACGACGAT 
GATCCAGTCA GAGAAGTTAA CACCGATTAC ATGTCTCTCC AGCCAGAAAG TAGATCGATT 
ACTACCCCGA GCAAGTCTCC TTATTTCAGC AATGGAAATA CCGAACCGTT AACGCCTACG 
AAATATATTT CTAAGGAGGA AAGAATCTTG GATACTGGTG GTACCTCGTT TCTGAACTAT 
CCCAGCGAAT CTTCCGACCT TCTTCGTCGA AAATACGACG CTTTGTCTCC TAAGGAGTAC 
ACTCATACCA AGAGTACGAC GGATTATGAT AGACACTTAG GCACGTTACC TCCTATAGAA 
CCTGGAACAG AGACAGGATC GTATTTACCG ACGAGACACC GAGATTATCA TTCTCTATCA 
CCGAATCGTC AGTTTCCTGA TCACTTTCCT TCTACTATCA ATCATCACTA CACCTCGAAA 
CTTTCATCAG GTCTCTCTGA CGAATCGAAA CGACCGGTTT CTCATCCGGA ATTCCCTGGC 
AAGATGAATG TTGGGAAATA TGCAGAATTA CCCGATAGAA GTTCCAGCAG TTACAAGAAA 
TCATCTCTTA GCTTAGGTAA CATAGGACAC TCGAGTAAAG GAGCTGATAA TAATTTCAAC 
ACTGTGACCA GTCCAAAAAC GCAGTTCAGT CCCTTTCCCG TTAGAAACGC TCCTAGGAAG 
CCGAAAGAAC TTACGCTGAA ATTGGGCCTC TATTCGCCCA AAGGTTCTGA TACAGGACAG 
TTAAAAAGGT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024224633.1
CDS	1744..8298
Translation	MSCNVMPLKKYAEISYRVKCVAVENERVFGDVYVTQQTRNLLYCADGYQFPSLYNRSRRNLIQKSWEGPDFMELNRKKPIRRASCTPPKVERTNLKRSKSLGDTWDVIETDCKNANNSKDENYICKPKTYPSPLPNPPENKKGTVSSLVDQLLLDIYGLPDDDRRRSESDSTASSLKIRPQHQHLQKARLLWKSEHELQVLVLNLRDHINHTGEILIRQLRRKDHLATKCEKLCAIITAHLLAVSPKCVEDTKMRFSLTPQPGESGFIQWLDAMKMVARLPGGIPPEFRKKLWLTLAERHLEQRGVDWKQAEKICFNEWSNPDDEELGIQIVKDLHRTGCSLFCGAAGRDNQAVLRRVLLGFARWNKSVGYCQGLNVLAALVLQVMDRAESAAVKVMIYLIEGVLPEGYFADNLRGLSVDMAVFRDLLRGRLPKLSKHLEALQNDAKDKATGSSYEPPLTNVFTMQWFLTLFCHCLPQEAVLRVWDLIFLEGDEILLRTALAIWEGLSDRIMTVTSADEFYSIMGVLTREMLEFTDTNNLIKNIVSMGSLHGVTGLREKHRYNITPWARKLSDDEDSDTEEDERLAVAAAMFSMAQRLKKDRIPQTIGALQAMAPSSDRERLALDITTLKQQYAKLRERQRQAHIILSVGKSALVSGKNRPLSLPSGAATTKIRPTTLNQNRRDRQGVTLHWKDTKKPKQKPSNLPETVECSVVTMQSTEAELASPKRSNGDSDSDSTSTELCDEPDRLSDVDSEDLTSASESCVVSTDEEKSSRITGSPIREKPLDRSFLEEKMEPINAEDSKDSKSSEDNLGDISIAKITDQIRRLSAEDDNNSMVPQKFSVQSKESPEEQSTKESKKEKSISDLSIDYTSDSNIIKKSLLDLNEYDYLGFSGSKISSKEDEVLVHERHRSKQTTNEIVDIPRNEVATIKKDQTEDKITTTMKQDSLNIYLDYSASLDKKYDRIKDKSSLDVEIENEIRSDRYKTSFDFSSRISLDLKPYETSKVGSLTSDTADTVNTERTLYGKAYVGHLPISPVTVDQKLSRVTPSIHTTHNTTVLTPETPEIVHKTLENTSYNDRIQTTQTKIYSDLVKSPKTPESNKSFSSGETMGPDSKPSSLTVSPRTPVRSESRDFPMDKSLCSSVSSDSKTLVLHSADSDSTRDSTKTDAKKMYEISNSATPISMDSLQKSEASPKLVVSSSSESCSPSKLKSSERSFSSDLQSPISANSIKYTSNFSRRGQDDVSGSPMDLSSATSPFYPISNPNQKTPTSPYSASRRRSSLDSNETSPEQKSTSSRESNKFLGYQSKFDSSIKTSDMKDVDGIARKGDTFVRPEFITRNEDSSINLTDRRINVSDVKYYQTTSTDYYRKDKDIFDDGGDDPSSEKDVVPSLPQEKLDKHYLNYRYRDRSKLLERSSSSLDSRRYADMKSSASTDEFSTSIAQKRSSDILEDIKHLEAKANDGSSDNGMLTKKSSYIWEDMKNLEARRQDTFSDSARNFSKHRLEVPDINITGESRKSYVVHPKINIDENSDSILKTMACGKSNGTDDGRESKLGVWTKVKPRKKSDNGRRNSDRALKIIQENSAILQKILACQAKKRLPDLEEISKEITISPINEEISKIFSPILEKMGLNEHEINEELARINFKDFDNMTATSVSEFDAKINEELSKLSLIDETEQMDHFDVDEVISHEYLNTREALIDRKINEELSKLLANYDDHSPASMVSLDKGPSSQNISEIEGLDLSSISTNVFSYKSSNDSIDTRSDLGSTQEPSILVDKFPKYTAYAVPKYHVDDSSPKSDIDIYRELEKLDKISSAQVLPDPTLELPQKELSSIPYVPNTSPFKDVSPLRYTSKPAQYDTSPLKTSYDPYKTFDFKPKLSPKQVSTNPFVSATYEKPVVDTAFEYINNKPEISINTYDLHLKSPMMTKESLEFRVRYDDDPVREVNTDYMSLQPESRSITTPSKSPYFSNGNTEPLTPTKYISKEERILDTGGTSFLNYPSESSDLLRRKYDALSPKEYTHTKSTTDYDRHLGTLPPIEPGTETGSYLPTRHRDYHSLSPNRQFPDHFPSTINHHYTSKLSSGLSDESKRPVSHPEFPGKMNVGKYAELPDRSSSSYKKSSLSLGNIGHSSKGADNNFNTVTSPKTQFSPFPVRNAPRKPKELTLKLGLYSPKGSDTGQLKRS

Target ORF information:

RefSeq Version	XM_024368865.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens uncharacterized LOC100749006 (LOC100749006), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024368865.1

ORF Insert Sequence:

ATGAGCTGTA ACGTGATGCC TCTGAAGAAG TACGCGGAAA TTAGTTACCG CGTGAAATGT 
GTGGCCGTGG AAAATGAGCG CGTGTTTGGT GACGTGTACG TCACACAACA AACACGAAAT 
TTGCTGTATT GCGCAGATGG ATATCAGTTT CCCTCGTTGT ATAACAGAAG CAGACGCAAT 
CTGATTCAGA AATCATGGGA AGGTCCGGAT TTCATGGAAC TGAATCGGAA AAAGCCGATC 
AGAAGGGCTA GTTGCACGCC GCCCAAAGTG GAGAGAACAA ATTTGAAGAG GTCGAAATCT 
TTGGGTGACA CGTGGGACGT AATCGAAACT GATTGCAAAA ATGCTAACAA TTCGAAAGAT 
GAAAATTACA TTTGCAAGCC AAAGACATAT CCATCACCGT TACCGAATCC ACCCGAGAAC 
AAAAAAGGAA CTGTGTCTTC GTTAGTTGAT CAATTGTTGC TAGATATTTA TGGATTGCCA 
GACGATGACA GAAGACGGTC AGAAAGTGAC TCGACAGCCT CATCCCTGAA GATACGTCCG 
CAACATCAGC ACCTGCAGAA GGCGCGTTTA CTCTGGAAAA GTGAGCACGA GCTTCAAGTT 
TTAGTGCTGA ATTTACGCGA CCATATTAAT CACACTGGTG AAATACTCAT TCGACAGTTG 
CGTCGTAAAG ATCATCTCGC CACCAAATGT GAGAAACTAT GCGCCATAAT TACGGCTCAC 
CTTCTGGCAG TCAGCCCAAA ATGCGTCGAA GACACGAAGA TGAGATTTAG CCTGACGCCG 
CAACCAGGAG AAAGTGGTTT TATACAATGG CTGGACGCGA TGAAAATGGT TGCCAGGTTA 
CCAGGAGGAA TTCCGCCGGA ATTTCGAAAG AAGTTGTGGC TGACACTGGC AGAACGTCAT 
CTAGAGCAGC GCGGCGTAGA TTGGAAGCAG GCTGAAAAGA TCTGCTTCAA CGAGTGGAGC 
AATCCTGACG ATGAAGAGCT TGGTATACAA ATCGTTAAAG ACCTACACAG AACGGGCTGC 
AGTCTGTTTT GCGGTGCAGC TGGCAGAGAC AATCAAGCAG TGCTTCGTCG AGTTCTGCTT 
GGTTTCGCCC GATGGAACAA GTCCGTCGGC TATTGCCAGG GCCTGAACGT GTTGGCCGCC 
CTCGTTTTGC AAGTAATGGA TCGCGCCGAG TCCGCCGCGG TGAAGGTGAT GATCTACTTG 
ATAGAGGGTG TCCTGCCCGA GGGTTACTTC GCGGACAATC TCCGTGGCCT GTCGGTCGAC 
ATGGCGGTAT TTCGAGATCT CCTCCGGGGC AGACTGCCCA AGCTCTCGAA ACATCTGGAG 
GCTCTGCAGA ACGATGCTAA GGACAAGGCG ACAGGCAGCA GTTACGAGCC ACCTCTGACG 
AATGTGTTCA CGATGCAATG GTTCCTTACT CTTTTCTGTC ACTGTTTGCC ACAAGAGGCT 
GTCCTGCGCG TATGGGACCT CATATTTCTC GAAGGAGATG AGATATTGCT TAGAACCGCA 
CTGGCCATCT GGGAAGGACT TTCCGATCGT ATTATGACGG TGACATCGGC GGACGAGTTT 
TATAGTATAA TGGGAGTGCT GACAAGGGAG ATGCTCGAGT TCACGGATAC TAATAACCTC 
ATAAAGAATA TCGTTAGTAT GGGATCTTTG CATGGTGTAA CGGGGCTAAG AGAGAAACAT 
AGATACAATA TCACACCTTG GGCAAGAAAA TTGAGCGACG ACGAGGACAG CGACACGGAG 
GAGGATGAAA GGCTGGCCGT GGCTGCTGCG ATGTTCAGTA TGGCACAGCG ATTGAAGAAA 
GATCGTATAC CTCAAACGAT TGGAGCGCTG CAGGCAATGG CACCCAGCAG CGACCGAGAA 
CGGCTCGCTT TGGACATTAC CACTTTGAAA CAGCAGTACG CCAAGCTACG CGAACGTCAA 
CGACAAGCGC ATATTATACT TTCTGTGGGC AAAAGTGCGC TCGTGAGTGG GAAGAATCGA 
CCCCTGAGCC TACCTTCTGG TGCTGCAACG ACGAAGATAA GACCTACGAC GCTAAATCAA 
AACCGAAGGG ACAGACAAGG CGTCACGCTT CACTGGAAAG ACACCAAAAA ACCGAAGCAG 
AAACCCTCGA ATTTACCAGA AACGGTGGAA TGTAGCGTTG TAACAATGCA ATCCACGGAA 
GCTGAGCTGG CTTCTCCAAA GCGGAGTAAC GGTGACAGTG ATAGCGACAG CACATCGACG 
GAATTGTGCG ACGAACCGGA CCGTCTCAGT GACGTTGACA GCGAAGATCT GACGTCAGCA 
TCCGAATCCT GTGTTGTCAG TACGGATGAG GAGAAAAGCT CTCGAATCAC CGGATCACCG 
ATTCGCGAGA AGCCACTCGA TCGTTCGTTT CTCGAAGAGA AGATGGAACC AATTAACGCC 
GAAGATTCGA AGGACTCGAA AAGCTCCGAG GATAACCTTG GTGACATATC AATCGCTAAA 
ATCACCGACC AAATAAGACG ATTATCAGCA GAGGATGACA ATAATTCCAT GGTTCCTCAA 
AAATTTAGCG TGCAGAGCAA AGAATCGCCG GAAGAACAAT CGACGAAAGA ATCGAAAAAA 
GAGAAATCCA TATCAGATCT ATCTATAGAT TACACTTCCG ATAGTAATAT CATTAAAAAA 
TCGTTATTGG ACTTGAACGA GTACGATTAT CTAGGTTTCA GTGGCAGTAA GATCAGTTCG 
AAAGAAGATG AAGTATTAGT GCACGAAAGG CATCGATCTA AACAGACAAC AAACGAGATT 
GTAGATATAC CTCGAAACGA AGTTGCGACA ATCAAGAAAG ACCAAACTGA AGACAAGATA 
ACTACGACTA TGAAACAGGA TTCTTTAAAC ATATACTTGG ATTATTCAGC ATCCTTGGAC 
AAGAAATACG ATAGAATCAA AGATAAATCA AGCCTCGATG TAGAGATAGA AAATGAAATT 
AGATCCGACC GATATAAAAC ATCCTTCGAT TTCTCTAGTA GAATATCTTT GGACTTGAAA 
CCGTATGAAA CTTCAAAAGT AGGAAGTTTA ACCAGTGACA CTGCAGACAC AGTGAATACT 
GAAAGAACGC TTTATGGCAA GGCCTACGTT GGTCATCTTC CTATTTCACC TGTAACAGTT 
GATCAAAAAT TAAGCAGAGT GACACCCAGT ATACATACAA CGCATAATAC TACTGTATTA 
ACTCCTGAAA CGCCTGAAAT TGTACATAAA ACGTTAGAGA ACACGTCATA TAACGATAGA 
ATACAGACGA CTCAAACGAA AATTTATTCA GACCTAGTGA AGAGTCCTAA AACACCGGAA 
AGCAACAAAT CATTCAGTTC AGGTGAAACC ATGGGACCAG ATTCGAAACC ATCGAGTCTG 
ACAGTTAGCC CAAGGACGCC GGTTAGATCA GAATCTCGAG ATTTCCCTAT GGACAAATCC 
CTGTGTTCGT CAGTTAGCTC AGACTCGAAG ACTTTGGTTC TTCATTCTGC AGATTCAGAC 
AGTACGAGGG ACAGCACGAA AACCGACGCG AAGAAAATGT ATGAGATTTC CAATTCGGCG 
ACACCTATCA GCATGGACTC GTTGCAGAAA TCAGAAGCCA GCCCAAAACT AGTCGTGAGC 
AGCTCCAGTG AATCGTGTAG CCCGAGTAAA TTAAAGTCTT CCGAGAGGTC GTTCAGTTCG 
GACCTGCAAT CGCCAATAAG TGCCAACTCC ATAAAGTATA CTTCGAATTT CAGTAGACGG 
GGCCAAGATG ACGTATCAGG CTCGCCAATG GACTTGAGCA GCGCTACCTC GCCGTTTTAT 
CCGATCTCGA ATCCGAATCA AAAAACTCCG ACGTCCCCGT ACAGTGCTTC TAGAAGAAGA 
TCCAGTTTGG ACAGCAACGA AACGTCACCT GAGCAGAAAT CGACCAGTTC GAGGGAGTCG 
AACAAGTTTC TAGGTTATCA GTCGAAATTT GACTCCTCCA TAAAGACGTC TGATATGAAA 
GATGTAGACG GTATAGCACG AAAAGGTGAC ACATTCGTCA GACCAGAGTT CATCACGAGG 
AATGAAGATT CGAGTATTAA TTTGACGGAT AGAAGAATCA ATGTTAGTGA TGTTAAGTAT 
TATCAAACAA CGAGTACGGA TTACTATCGC AAGGATAAGG ATATTTTTGA TGACGGAGGG 
GATGATCCGT CATCGGAGAA AGACGTGGTA CCTTCTTTGC CTCAGGAGAA GCTAGATAAA 
CATTATTTGA ACTATCGATA TAGAGATAGA TCGAAGTTGT TGGAAAGAAG TTCAAGCAGT 
TTGGACAGTA GAAGGTACGC TGATATGAAG TCATCGGCTT CCACCGATGA ATTTTCAACG 
TCGATCGCGC AGAAGAGATC CAGTGACATT TTAGAAGATA TTAAACACTT GGAGGCGAAA 
GCAAACGATG GGTCGTCGGA TAATGGGATG TTGACGAAGA AGAGCAGTTA TATTTGGGAG 
GATATGAAGA ATTTAGAAGC CAGGAGGCAA GATACCTTTA GTGATTCAGC GAGGAACTTT 
TCGAAACATC GTCTGGAGGT ACCGGATATA AATATAACCG GAGAAAGCAG AAAATCGTAC 
GTAGTACATC CGAAGATCAA TATCGACGAA AATAGCGACA GCATATTGAA GACCATGGCC 
TGTGGTAAGA GTAACGGCAC AGATGATGGC AGGGAGTCGA AGTTGGGCGT GTGGACGAAG 
GTGAAGCCTC GTAAAAAGAG TGACAATGGG CGGAGGAACA GCGACAGGGC GTTGAAGATC 
ATTCAAGAAA ACTCCGCTAT ACTTCAGAAG ATTCTTGCGT GTCAGGCAAA AAAGCGTCTG 
CCAGACCTGG AGGAGATATC GAAAGAAATT ACCATTAGCC CTATAAACGA GGAGATTTCG 
AAGATCTTTA GTCCGATATT AGAGAAAATG GGATTAAACG AACACGAGAT TAACGAGGAA 
TTGGCGAGAA TCAATTTCAA GGATTTCGAC AACATGACCG CTACCAGTGT GTCCGAGTTT 
GATGCAAAGA TTAACGAGGA ATTATCGAAG CTTTCTCTGA TCGACGAGAC GGAGCAGATG 
GACCACTTCG ACGTGGACGA GGTGATATCC CACGAATACT TGAACACGAG AGAAGCTCTA 
ATAGATCGTA AGATAAACGA GGAATTGTCG AAATTATTGG CAAACTACGA CGATCATTCT 
CCGGCTAGTA TGGTGAGTTT GGACAAAGGA CCATCCAGCC AGAATATTAG CGAGATCGAA 
GGACTGGATC TGTCCAGTAT TTCAACTAAC GTATTTTCCT ATAAATCATC CAATGACTCC 
ATTGACACGA GAAGCGACTT AGGCTCGACG CAGGAACCAT CCATTCTCGT TGACAAGTTT 
CCGAAATATA CGGCATACGC CGTACCGAAG TATCACGTGG ACGACTCATC ACCTAAAAGC 
GACATAGACA TCTACAGAGA ATTGGAGAAA CTCGATAAGA TCTCTTCCGC ACAGGTGTTA 
CCAGATCCGA CTCTGGAGCT TCCACAGAAG GAATTGTCCT CGATTCCGTA CGTTCCAAAT 
ACGTCGCCCT TCAAGGATGT GTCACCATTG AGGTACACTT CCAAGCCGGC TCAATACGAC 
ACTTCACCCC TGAAGACTTC TTACGATCCC TACAAGACCT TTGACTTCAA GCCCAAGTTG 
TCACCTAAAC AAGTTTCCAC TAATCCATTT GTGTCAGCTA CGTATGAGAA ACCTGTGGTG 
GATACCGCTT TCGAGTACAT TAACAATAAA CCAGAGATCT CGATCAATAC GTATGATCTA 
CATTTGAAGA GTCCAATGAT GACGAAGGAA TCGCTAGAGT TTCGTGTCAG ATACGACGAT 
GATCCAGTCA GAGAAGTTAA CACCGATTAC ATGTCTCTCC AGCCAGAAAG TAGATCGATT 
ACTACCCCGA GCAAGTCTCC TTATTTCAGC AATGGAAATA CCGAACCGTT AACGCCTACG 
AAATATATTT CTAAGGAGGA AAGAATCTTG GATACTGGTG GTACCTCGTT TCTGAACTAT 
CCCAGCGAAT CTTCCGACCT TCTTCGTCGA AAATACGACG CTTTGTCTCC TAAGGAGTAC 
ACTCATACCA AGAGTACGAC GGATTATGAT AGACACTTAG GCACGTTACC TCCTATAGAA 
CCTGGAACAG AGACAGGATC GTATTTACCG ACGAGACACC GAGATTATCA TTCTCTATCA 
CCGAATCGTC AGTTTCCTGA TCACTTTCCT TCTACTATCA ATCATCACTA CACCTCGAAA 
CTTTCATCAG GTCTCTCTGA CGAATCGAAA CGACCGGTTT CTCATCCGGA ATTCCCTGGC 
AAGATGAATG TTGGGAAATA TGCAGAATTA CCCGATAGAA GTTCCAGCAG TTACAAGAAA 
TCATCTCTTA GCTTAGGTAA CATAGGACAC TCGAGTAAAG GAGCTGATAA TAATTTCAAC 
ACTGTGACCA GTCCAAAAAC GCAGTTCAGT CCCTTTCCCG TTAGAAACGC TCCTAGGAAG 
CCGAAAGAAC TTACGCTGAA ATTGGGCCTC TATTCGCCCA AAGGTTCTGA TACAGGACAG 
TTAAAAAGGT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

LOC100749006 cDNA ORF clone, Bombus impatiens(common eastern bumble bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム