LOC100743105 cDNA ORF clone, Bombus impatiens(common eastern bumble bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100743105
Entrez Gene ID	100743105
Full Name	RNA-binding protein 33
Gene Type	protein-coding
Organism	Bombus impatiens(common eastern bumble bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012386057.1	XP_012241480.1	RNA-binding protein 33
XM_012386057.2	XP_012241480.1	RNA-binding protein 33

Pathways from BioSystems
Gene Ontology

The following LOC100743105 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100743105 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBo104232	XM_012386057.2 Latest version!	Bombus impatiens RNA-binding protein 33 (LOC100743105), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OBo104232	XM_012386057.1	Bombus impatiens RNA-binding protein 33 (LOC100743105), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100743105 ( XM_012386057.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012241480.1 Bombus impatiens RNA-binding protein 33 (LOC100743105), mRNA.

Price & Availability↑

CloneID	OBo104232
Clone ID Related Accession (Same CDS sequence)	XM_012386057.1 , XM_012386057.2
Accession Version	XM_012386057.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	RNA-binding protein 33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176796.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCGCTTCT TTAAACTGAT TGTCACGATA ATAGCGCTCT TCGTCGTTGC GACCTTTGCC 
AAAGATGAGT CCTCGAAAAG CCGCGATAAG AGGCAACAGG TTGCATTCTA TCCGAGAAGA 
GGAGCCAGAC CGCCACCCTA TGCCGTCCAA ATGGAACCAA TCGTTCAATA TTCTCAGAGA 
GATCCGCTCT ACAATCCTTT GGCAAGCTCC AAGAGCGACG ATCTTTACGA AGAGGGTCCG 
TTGAATTATT ATCCGACGGA ACCGGAGCCT ATCATCGAAA TTATCATCAA GGAATCCAAC 
GAAACGTTGC CGACACCGGT ACCCCCACCA CCTCCTCCAC CGCCAAGACC TACGAAAGAG 
CCTATACAAG TGTTTTATGT GAAATACGAG AAAAAGAAAG GTTACGGGGA TAAGTCTCGA 
GTCGTCTACG ATCCACCCGT GCCTGCGCTA ACACCTGTCG AGGAACACGA GGAGATCAAG 
CCAGATAACC CAGCGCCTTA CCCGGAAGAA CCTGTTCACA CGGCGACACC GGCACCGTCA 
GAACCGTCTA CGACTTTGAG AGCTATCATT CGACCCGACA GTGAAGTGTA CCATTCTGGT 
TCTAGCGGTA TTCGCGTCAC CTTCGGCACC GATCAAGTAC CTCCGAACAA TCATCATAAG 
CGTAGCGATT TGGAAGCGCC GCCGACCCAA CAACAGGCCA AGCCGACTTC TACCCCTCCA 
GGATCACCTA AACGACAACA TGGTCCGTAT CAACCGATAC AACCGGTCCA TCAGAGAGTT 
CCACCACCAT TTCCACAGCA GAGAATCGCC AACTCTTTTC CACAACAGCA GGCACTCCAT 
CAACCTCTCC AACAGCAACC GCCTCCTAAT TTCTTGCAGC AACAGCCGTC CTTCTCGTCT 
CCGCCGCAAG CGAGGAGTCC GTTGCAAAGA CCTCAACGAT TGCCTATAAC GATTCAAGGT 
CTACCAGATG TACAACAACG AGCGCCGTTC AATACTTTCG GTACATCTCA TCGCGTCAAC 
ATTAACCACC CACAACAACC AGGGTTCCCA CCTACGTTGT CGGTTTCCCA TCAAAACGTG 
CAGGTACAAA ATCAACCTTT ACCACCGAAC CAGGGCAGTC CTTTCAACGT GGAACAACAG 
CCGCAGCAGC CACCTTTGTC CTCGTATAAA CAACAAGAAT TAGAAAAACA AAGGTATCAC 
GAGCAACGTC GTCAACAGGA ATTGCTAAAG CATCGCGAAC ATCAGAGGCA ACACGAGCAA 
CAAAGACTTC AGGAGCATCA ACGATTCTTG GAACAACAGA GGATACTGGA TCAGCAGAGG 
CAGCACGAAC AACAGAGACA ACAGGATCAT CAAAGATTGC AGGAGCAACA GAGAATACAG 
GAGCAGCAGA GGATACAGGA ACAGCAGAGG ATACAGGAAC AGCAGAGGAT ACAGGAACAG 
CAGAGGATAC AGGAGCAACA GAGATTACAA GACCAACAGA GATTGCAGGA ACAGCAAAGA 
TTACAGGAGC AACAAAGGCT GCAGGAACAA CGCAGAAGGA AACAAGAACA AGAACAGAAA 
TTGTTACAGG AGCAACAGTA TCGTTCGCAA TTGAAATATA ATCAAGCGCA ACCGGTGCAC 
AATATACCGC AAGTACCAAT CTTGCCTCAA ATTCAAAAAC CAGGAGGAGA AATTTTCAAA 
GCTGTTCCGA AGCTCGAGCA ACACTATGCG ATTCGAGAAA ATCCACTTCA TCCTGGCCCT 
TTCCCACCTA ATCCTGTAAT CCAGCCAACT GGATTCCCGG ACACGCCACA GAATCAATAC 
GTTTCCGCTC AACCGCCGAG TTCGTCGCCA GACATCTTAA GAAACCCACC GAGCGAATTT 
ATAAGCGATC AACAGCAACA TCTGAATACG AAACAGCAAA TTATTCAAAG TCAGCAACAA 
GGATTTTTCT CTCAAAATCT TCAACAAGCG CAACAGCAGC AAAGATTCCC AGCGTCTCCA 
CAACGATCTT CGATCTGGGG ACGTCCATCG TTTGCCGGAA ACAGCGCGAG TCCTTCGCAA 
AAGATTTACG CGACTCCAGT CAGTCCCTCT GAAGAATTTA ATTCGATCTC AACCATCAGA 
CCGTTCGTTC CAAGTACAAC TACTACTACC ACGACGACGA CAACGACGAC TACCACCGAA 
GCTCCAACGA CGACAACAAC TTTCTCTCCA AAGAAAGAGG CTAAAATTAG GGAAAATATC 
GCGAATCTTC CGGACGAGGT ACCGGATGAT ATCAGGGAAC AGCTGCTGAG TTCCGGTATT 
TTGGGTAATG CCGACATACA AATACTGGAT TACGACAAGG TCGGCGACAT ACCGATAGAG 
AAACTACCGC CGGAGGCTCT GGCGAATTTC TACGGTGCCG GAGGCGGTTC CGCGGTAGCT 
GCCAGCGAAC CGGTCCCGTC GATCGTCAAG AAACCCCAAA CGGCAGAGAG CGCTTCCTCC 
TCGACCTCCT CTTCTTCCTC CACTTCCTCG CCAGTTTCGG CGAAGAAGGC GGCTACCGAT 
AGCTCGACTA AGTTCGAGCA GGCTACCTTG CGGCCAGGTG GAGTGGAGAT GAAAGTAGTA 
CGCTTCGATC CAAATACGGA GCAAGGCCAG GCGCTAGCGG ATCAACACAT ACGCGATGAT 
GCTACCAGGT TGAATCCCGT AACCGTAGGA GAGAAAGACG AGTCCCAATA TAATCGTTAT 
CTACCGCTCA AAGTGAGTGG TGCTTCCTTC CCTATTCCGG ACGTGCCGCA ACTTAATGGT 
CGGAAGATCT CTAGCGTAGT TGTATTGGCA CCCGTCGACT ATAACTTTCA TGAGGAAAAA 
GAGATTGAAT CGAGGCAAGG CAGAAGGGTC AGCGATGTGC AAGCGGTCAA GTTTCTCGCC 
GGCGATACTC TGAAACAACT GGTGAAAAAA CCAACGGCGG ACAACTACAA GAAGTGGTTG 
GAGCAGGAAA GCCGAACGGA GCCGCAGAAA CAGTCGGTGG TTCTATTGGT AACCATGCCA 
GAGGGCGCGG ATCAAGGCGA GAAGGAAATC TTCATGTATG ATGTGACCTC GCAAACTGTG 
AGCAAACTGG CCGGAGATCT ATCTACAGCG TTTGTAGATG CTGCTGAAAG TAACTCCGAC 
AATACCGACT CTTTCACCGA TTCAACTCTT TCATCGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012241480.1
CDS	64..3225
Translation	MRFFKLIVTIIALFVVATFAKDESSKSRDKRQQVAFYPRRGARPPPYAVQMEPIVQYSQRDPLYNPLASSKSDDLYEEGPLNYYPTEPEPIIEIIIKESNETLPTPVPPPPPPPPRPTKEPIQVFYVKYEKKKGYGDKSRVVYDPPVPALTPVEEHEEIKPDNPAPYPEEPVHTATPAPSEPSTTLRAIIRPDSEVYHSGSSGIRVTFGTDQVPPNNHHKRSDLEAPPTQQQAKPTSTPPGSPKRQHGPYQPIQPVHQRVPPPFPQQRIANSFPQQQALHQPLQQQPPPNFLQQQPSFSSPPQARSPLQRPQRLPITIQGLPDVQQRAPFNTFGTSHRVNINHPQQPGFPPTLSVSHQNVQVQNQPLPPNQGSPFNVEQQPQQPPLSSYKQQELEKQRYHEQRRQQELLKHREHQRQHEQQRLQEHQRFLEQQRILDQQRQHEQQRQQDHQRLQEQQRIQEQQRIQEQQRIQEQQRIQEQQRIQEQQRLQDQQRLQEQQRLQEQQRLQEQRRRKQEQEQKLLQEQQYRSQLKYNQAQPVHNIPQVPILPQIQKPGGEIFKAVPKLEQHYAIRENPLHPGPFPPNPVIQPTGFPDTPQNQYVSAQPPSSSPDILRNPPSEFISDQQQHLNTKQQIIQSQQQGFFSQNLQQAQQQQRFPASPQRSSIWGRPSFAGNSASPSQKIYATPVSPSEEFNSISTIRPFVPSTTTTTTTTTTTTTTEAPTTTTTFSPKKEAKIRENIANLPDEVPDDIREQLLSSGILGNADIQILDYDKVGDIPIEKLPPEALANFYGAGGGSAVAASEPVPSIVKKPQTAESASSSTSSSSSTSSPVSAKKAATDSSTKFEQATLRPGGVEMKVVRFDPNTEQGQALADQHIRDDATRLNPVTVGEKDESQYNRYLPLKVSGASFPIPDVPQLNGRKISSVVVLAPVDYNFHEEKEIESRQGRRVSDVQAVKFLAGDTLKQLVKKPTADNYKKWLEQESRTEPQKQSVVLLVTMPEGADQGEKEIFMYDVTSQTVSKLAGDLSTAFVDAAESNSDNTDSFTDSTLSSF

Target ORF information:

RefSeq Version	XM_012386057.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens RNA-binding protein 33 (LOC100743105), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012386057.1

ORF Insert Sequence:

ATGCGCTTCT TTAAACTGAT TGTCACGATA ATAGCGCTCT TCGTCGTTGC GACCTTTGCC 
AAAGATGAGT CCTCGAAAAG CCGCGATAAG AGGCAACAGG TTGCATTCTA TCCGAGAAGA 
GGAGCCAGAC CGCCACCCTA TGCCGTCCAA ATGGAACCAA TCGTTCAATA TTCTCAGAGA 
GATCCGCTCT ACAATCCTTT GGCAAGCTCC AAGAGCGACG ATCTTTACGA AGAGGGTCCG 
TTGAATTATT ATCCGACGGA ACCGGAGCCT ATCATCGAAA TTATCATCAA GGAATCCAAC 
GAAACGTTGC CGACACCGGT ACCCCCACCA CCTCCTCCAC CGCCAAGACC TACGAAAGAG 
CCTATACAAG TGTTTTATGT GAAATACGAG AAAAAGAAAG GTTACGGGGA TAAGTCTCGA 
GTCGTCTACG ATCCACCCGT GCCTGCGCTA ACACCTGTCG AGGAACACGA GGAGATCAAG 
CCAGATAACC CAGCGCCTTA CCCGGAAGAA CCTGTTCACA CGGCGACACC GGCACCGTCA 
GAACCGTCTA CGACTTTGAG AGCTATCATT CGACCCGACA GTGAAGTGTA CCATTCTGGT 
TCTAGCGGTA TTCGCGTCAC CTTCGGCACC GATCAAGTAC CTCCGAACAA TCATCATAAG 
CGTAGCGATT TGGAAGCGCC GCCGACCCAA CAACAGGCCA AGCCGACTTC TACCCCTCCA 
GGATCACCTA AACGACAACA TGGTCCGTAT CAACCGATAC AACCGGTCCA TCAGAGAGTT 
CCACCACCAT TTCCACAGCA GAGAATCGCC AACTCTTTTC CACAACAGCA GGCACTCCAT 
CAACCTCTCC AACAGCAACC GCCTCCTAAT TTCTTGCAGC AACAGCCGTC CTTCTCGTCT 
CCGCCGCAAG CGAGGAGTCC GTTGCAAAGA CCTCAACGAT TGCCTATAAC GATTCAAGGT 
CTACCAGATG TACAACAACG AGCGCCGTTC AATACTTTCG GTACATCTCA TCGCGTCAAC 
ATTAACCACC CACAACAACC AGGGTTCCCA CCTACGTTGT CGGTTTCCCA TCAAAACGTG 
CAGGTACAAA ATCAACCTTT ACCACCGAAC CAGGGCAGTC CTTTCAACGT GGAACAACAG 
CCGCAGCAGC CACCTTTGTC CTCGTATAAA CAACAAGAAT TAGAAAAACA AAGGTATCAC 
GAGCAACGTC GTCAACAGGA ATTGCTAAAG CATCGCGAAC ATCAGAGGCA ACACGAGCAA 
CAAAGACTTC AGGAGCATCA ACGATTCTTG GAACAACAGA GGATACTGGA TCAGCAGAGG 
CAGCACGAAC AACAGAGACA ACAGGATCAT CAAAGATTGC AGGAGCAACA GAGAATACAG 
GAGCAGCAGA GGATACAGGA ACAGCAGAGG ATACAGGAAC AGCAGAGGAT ACAGGAACAG 
CAGAGGATAC AGGAGCAACA GAGATTACAA GACCAACAGA GATTGCAGGA ACAGCAAAGA 
TTACAGGAGC AACAAAGGCT GCAGGAACAA CGCAGAAGGA AACAAGAACA AGAACAGAAA 
TTGTTACAGG AGCAACAGTA TCGTTCGCAA TTGAAATATA ATCAAGCGCA ACCGGTGCAC 
AATATACCGC AAGTACCAAT CTTGCCTCAA ATTCAAAAAC CAGGAGGAGA AATTTTCAAA 
GCTGTTCCGA AGCTCGAGCA ACACTATGCG ATTCGAGAAA ATCCACTTCA TCCTGGCCCT 
TTCCCACCTA ATCCTGTAAT CCAGCCAACT GGATTCCCGG ACACGCCACA GAATCAATAC 
GTTTCCGCTC AACCGCCGAG TTCGTCGCCA GACATCTTAA GAAACCCACC GAGCGAATTT 
ATAAGCGATC AACAGCAACA TCTGAATACG AAACAGCAAA TTATTCAAAG TCAGCAACAA 
GGATTTTTCT CTCAAAATCT TCAACAAGCG CAACAGCAGC AAAGATTCCC AGCGTCTCCA 
CAACGATCTT CGATCTGGGG ACGTCCATCG TTTGCCGGAA ACAGCGCGAG TCCTTCGCAA 
AAGATTTACG CGACTCCAGT CAGTCCCTCT GAAGAATTTA ATTCGATCTC AACCATCAGA 
CCGTTCGTTC CAAGTACAAC TACTACTACC ACGACGACGA CAACGACGAC TACCACCGAA 
GCTCCAACGA CGACAACAAC TTTCTCTCCA AAGAAAGAGG CTAAAATTAG GGAAAATATC 
GCGAATCTTC CGGACGAGGT ACCGGATGAT ATCAGGGAAC AGCTGCTGAG TTCCGGTATT 
TTGGGTAATG CCGACATACA AATACTGGAT TACGACAAGG TCGGCGACAT ACCGATAGAG 
AAACTACCGC CGGAGGCTCT GGCGAATTTC TACGGTGCCG GAGGCGGTTC CGCGGTAGCT 
GCCAGCGAAC CGGTCCCGTC GATCGTCAAG AAACCCCAAA CGGCAGAGAG CGCTTCCTCC 
TCGACCTCCT CTTCTTCCTC CACTTCCTCG CCAGTTTCGG CGAAGAAGGC GGCTACCGAT 
AGCTCGACTA AGTTCGAGCA GGCTACCTTG CGGCCAGGTG GAGTGGAGAT GAAAGTAGTA 
CGCTTCGATC CAAATACGGA GCAAGGCCAG GCGCTAGCGG ATCAACACAT ACGCGATGAT 
GCTACCAGGT TGAATCCCGT AACCGTAGGA GAGAAAGACG AGTCCCAATA TAATCGTTAT 
CTACCGCTCA AAGTGAGTGG TGCTTCCTTC CCTATTCCGG ACGTGCCGCA ACTTAATGGT 
CGGAAGATCT CTAGCGTAGT TGTATTGGCA CCCGTCGACT ATAACTTTCA TGAGGAAAAA 
GAGATTGAAT CGAGGCAAGG CAGAAGGGTC AGCGATGTGC AAGCGGTCAA GTTTCTCGCC 
GGCGATACTC TGAAACAACT GGTGAAAAAA CCAACGGCGG ACAACTACAA GAAGTGGTTG 
GAGCAGGAAA GCCGAACGGA GCCGCAGAAA CAGTCGGTGG TTCTATTGGT AACCATGCCA 
GAGGGCGCGG ATCAAGGCGA GAAGGAAATC TTCATGTATG ATGTGACCTC GCAAACTGTG 
AGCAAACTGG CCGGAGATCT ATCTACAGCG TTTGTAGATG CTGCTGAAAG TAACTCCGAC 
AATACCGACT CTTTCACCGA TTCAACTCTT TCATCGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100743105 ( XM_012386057.2 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012241480.1 Bombus impatiens RNA-binding protein 33 (LOC100743105), mRNA.

Price & Availability↑

CloneID	OBo104232
Clone ID Related Accession (Same CDS sequence)	XM_012386057.1 , XM_012386057.2
Accession Version	XM_012386057.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	RNA-binding protein 33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176796.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 19, 2018 this sequence version replaced XM_012386057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTTCT TTAAACTGAT TGTCACGATA ATAGCGCTCT TCGTCGTTGC GACCTTTGCC 
AAAGATGAGT CCTCGAAAAG CCGCGATAAG AGGCAACAGG TTGCATTCTA TCCGAGAAGA 
GGAGCCAGAC CGCCACCCTA TGCCGTCCAA ATGGAACCAA TCGTTCAATA TTCTCAGAGA 
GATCCGCTCT ACAATCCTTT GGCAAGCTCC AAGAGCGACG ATCTTTACGA AGAGGGTCCG 
TTGAATTATT ATCCGACGGA ACCGGAGCCT ATCATCGAAA TTATCATCAA GGAATCCAAC 
GAAACGTTGC CGACACCGGT ACCCCCACCA CCTCCTCCAC CGCCAAGACC TACGAAAGAG 
CCTATACAAG TGTTTTATGT GAAATACGAG AAAAAGAAAG GTTACGGGGA TAAGTCTCGA 
GTCGTCTACG ATCCACCCGT GCCTGCGCTA ACACCTGTCG AGGAACACGA GGAGATCAAG 
CCAGATAACC CAGCGCCTTA CCCGGAAGAA CCTGTTCACA CGGCGACACC GGCACCGTCA 
GAACCGTCTA CGACTTTGAG AGCTATCATT CGACCCGACA GTGAAGTGTA CCATTCTGGT 
TCTAGCGGTA TTCGCGTCAC CTTCGGCACC GATCAAGTAC CTCCGAACAA TCATCATAAG 
CGTAGCGATT TGGAAGCGCC GCCGACCCAA CAACAGGCCA AGCCGACTTC TACCCCTCCA 
GGATCACCTA AACGACAACA TGGTCCGTAT CAACCGATAC AACCGGTCCA TCAGAGAGTT 
CCACCACCAT TTCCACAGCA GAGAATCGCC AACTCTTTTC CACAACAGCA GGCACTCCAT 
CAACCTCTCC AACAGCAACC GCCTCCTAAT TTCTTGCAGC AACAGCCGTC CTTCTCGTCT 
CCGCCGCAAG CGAGGAGTCC GTTGCAAAGA CCTCAACGAT TGCCTATAAC GATTCAAGGT 
CTACCAGATG TACAACAACG AGCGCCGTTC AATACTTTCG GTACATCTCA TCGCGTCAAC 
ATTAACCACC CACAACAACC AGGGTTCCCA CCTACGTTGT CGGTTTCCCA TCAAAACGTG 
CAGGTACAAA ATCAACCTTT ACCACCGAAC CAGGGCAGTC CTTTCAACGT GGAACAACAG 
CCGCAGCAGC CACCTTTGTC CTCGTATAAA CAACAAGAAT TAGAAAAACA AAGGTATCAC 
GAGCAACGTC GTCAACAGGA ATTGCTAAAG CATCGCGAAC ATCAGAGGCA ACACGAGCAA 
CAAAGACTTC AGGAGCATCA ACGATTCTTG GAACAACAGA GGATACTGGA TCAGCAGAGG 
CAGCACGAAC AACAGAGACA ACAGGATCAT CAAAGATTGC AGGAGCAACA GAGAATACAG 
GAGCAGCAGA GGATACAGGA ACAGCAGAGG ATACAGGAAC AGCAGAGGAT ACAGGAACAG 
CAGAGGATAC AGGAGCAACA GAGATTACAA GACCAACAGA GATTGCAGGA ACAGCAAAGA 
TTACAGGAGC AACAAAGGCT GCAGGAACAA CGCAGAAGGA AACAAGAACA AGAACAGAAA 
TTGTTACAGG AGCAACAGTA TCGTTCGCAA TTGAAATATA ATCAAGCGCA ACCGGTGCAC 
AATATACCGC AAGTACCAAT CTTGCCTCAA ATTCAAAAAC CAGGAGGAGA AATTTTCAAA 
GCTGTTCCGA AGCTCGAGCA ACACTATGCG ATTCGAGAAA ATCCACTTCA TCCTGGCCCT 
TTCCCACCTA ATCCTGTAAT CCAGCCAACT GGATTCCCGG ACACGCCACA GAATCAATAC 
GTTTCCGCTC AACCGCCGAG TTCGTCGCCA GACATCTTAA GAAACCCACC GAGCGAATTT 
ATAAGCGATC AACAGCAACA TCTGAATACG AAACAGCAAA TTATTCAAAG TCAGCAACAA 
GGATTTTTCT CTCAAAATCT TCAACAAGCG CAACAGCAGC AAAGATTCCC AGCGTCTCCA 
CAACGATCTT CGATCTGGGG ACGTCCATCG TTTGCCGGAA ACAGCGCGAG TCCTTCGCAA 
AAGATTTACG CGACTCCAGT CAGTCCCTCT GAAGAATTTA ATTCGATCTC AACCATCAGA 
CCGTTCGTTC CAAGTACAAC TACTACTACC ACGACGACGA CAACGACGAC TACCACCGAA 
GCTCCAACGA CGACAACAAC TTTCTCTCCA AAGAAAGAGG CTAAAATTAG GGAAAATATC 
GCGAATCTTC CGGACGAGGT ACCGGATGAT ATCAGGGAAC AGCTGCTGAG TTCCGGTATT 
TTGGGTAATG CCGACATACA AATACTGGAT TACGACAAGG TCGGCGACAT ACCGATAGAG 
AAACTACCGC CGGAGGCTCT GGCGAATTTC TACGGTGCCG GAGGCGGTTC CGCGGTAGCT 
GCCAGCGAAC CGGTCCCGTC GATCGTCAAG AAACCCCAAA CGGCAGAGAG CGCTTCCTCC 
TCGACCTCCT CTTCTTCCTC CACTTCCTCG CCAGTTTCGG CGAAGAAGGC GGCTACCGAT 
AGCTCGACTA AGTTCGAGCA GGCTACCTTG CGGCCAGGTG GAGTGGAGAT GAAAGTAGTA 
CGCTTCGATC CAAATACGGA GCAAGGCCAG GCGCTAGCGG ATCAACACAT ACGCGATGAT 
GCTACCAGGT TGAATCCCGT AACCGTAGGA GAGAAAGACG AGTCCCAATA TAATCGTTAT 
CTACCGCTCA AAGTGAGTGG TGCTTCCTTC CCTATTCCGG ACGTGCCGCA ACTTAATGGT 
CGGAAGATCT CTAGCGTAGT TGTATTGGCA CCCGTCGACT ATAACTTTCA TGAGGAAAAA 
GAGATTGAAT CGAGGCAAGG CAGAAGGGTC AGCGATGTGC AAGCGGTCAA GTTTCTCGCC 
GGCGATACTC TGAAACAACT GGTGAAAAAA CCAACGGCGG ACAACTACAA GAAGTGGTTG 
GAGCAGGAAA GCCGAACGGA GCCGCAGAAA CAGTCGGTGG TTCTATTGGT AACCATGCCA 
GAGGGCGCGG ATCAAGGCGA GAAGGAAATC TTCATGTATG ATGTGACCTC GCAAACTGTG 
AGCAAACTGG CCGGAGATCT ATCTACAGCG TTTGTAGATG CTGCTGAAAG TAACTCCGAC 
AATACCGACT CTTTCACCGA TTCAACTCTT TCATCGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012241480.1
CDS	1213..4374
Translation	MRFFKLIVTIIALFVVATFAKDESSKSRDKRQQVAFYPRRGARPPPYAVQMEPIVQYSQRDPLYNPLASSKSDDLYEEGPLNYYPTEPEPIIEIIIKESNETLPTPVPPPPPPPPRPTKEPIQVFYVKYEKKKGYGDKSRVVYDPPVPALTPVEEHEEIKPDNPAPYPEEPVHTATPAPSEPSTTLRAIIRPDSEVYHSGSSGIRVTFGTDQVPPNNHHKRSDLEAPPTQQQAKPTSTPPGSPKRQHGPYQPIQPVHQRVPPPFPQQRIANSFPQQQALHQPLQQQPPPNFLQQQPSFSSPPQARSPLQRPQRLPITIQGLPDVQQRAPFNTFGTSHRVNINHPQQPGFPPTLSVSHQNVQVQNQPLPPNQGSPFNVEQQPQQPPLSSYKQQELEKQRYHEQRRQQELLKHREHQRQHEQQRLQEHQRFLEQQRILDQQRQHEQQRQQDHQRLQEQQRIQEQQRIQEQQRIQEQQRIQEQQRIQEQQRLQDQQRLQEQQRLQEQQRLQEQRRRKQEQEQKLLQEQQYRSQLKYNQAQPVHNIPQVPILPQIQKPGGEIFKAVPKLEQHYAIRENPLHPGPFPPNPVIQPTGFPDTPQNQYVSAQPPSSSPDILRNPPSEFISDQQQHLNTKQQIIQSQQQGFFSQNLQQAQQQQRFPASPQRSSIWGRPSFAGNSASPSQKIYATPVSPSEEFNSISTIRPFVPSTTTTTTTTTTTTTTEAPTTTTTFSPKKEAKIRENIANLPDEVPDDIREQLLSSGILGNADIQILDYDKVGDIPIEKLPPEALANFYGAGGGSAVAASEPVPSIVKKPQTAESASSSTSSSSSTSSPVSAKKAATDSSTKFEQATLRPGGVEMKVVRFDPNTEQGQALADQHIRDDATRLNPVTVGEKDESQYNRYLPLKVSGASFPIPDVPQLNGRKISSVVVLAPVDYNFHEEKEIESRQGRRVSDVQAVKFLAGDTLKQLVKKPTADNYKKWLEQESRTEPQKQSVVLLVTMPEGADQGEKEIFMYDVTSQTVSKLAGDLSTAFVDAAESNSDNTDSFTDSTLSSF

Target ORF information:

RefSeq Version	XM_012386057.2
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens RNA-binding protein 33 (LOC100743105), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012386057.2

ORF Insert Sequence:

ATGCGCTTCT TTAAACTGAT TGTCACGATA ATAGCGCTCT TCGTCGTTGC GACCTTTGCC 
AAAGATGAGT CCTCGAAAAG CCGCGATAAG AGGCAACAGG TTGCATTCTA TCCGAGAAGA 
GGAGCCAGAC CGCCACCCTA TGCCGTCCAA ATGGAACCAA TCGTTCAATA TTCTCAGAGA 
GATCCGCTCT ACAATCCTTT GGCAAGCTCC AAGAGCGACG ATCTTTACGA AGAGGGTCCG 
TTGAATTATT ATCCGACGGA ACCGGAGCCT ATCATCGAAA TTATCATCAA GGAATCCAAC 
GAAACGTTGC CGACACCGGT ACCCCCACCA CCTCCTCCAC CGCCAAGACC TACGAAAGAG 
CCTATACAAG TGTTTTATGT GAAATACGAG AAAAAGAAAG GTTACGGGGA TAAGTCTCGA 
GTCGTCTACG ATCCACCCGT GCCTGCGCTA ACACCTGTCG AGGAACACGA GGAGATCAAG 
CCAGATAACC CAGCGCCTTA CCCGGAAGAA CCTGTTCACA CGGCGACACC GGCACCGTCA 
GAACCGTCTA CGACTTTGAG AGCTATCATT CGACCCGACA GTGAAGTGTA CCATTCTGGT 
TCTAGCGGTA TTCGCGTCAC CTTCGGCACC GATCAAGTAC CTCCGAACAA TCATCATAAG 
CGTAGCGATT TGGAAGCGCC GCCGACCCAA CAACAGGCCA AGCCGACTTC TACCCCTCCA 
GGATCACCTA AACGACAACA TGGTCCGTAT CAACCGATAC AACCGGTCCA TCAGAGAGTT 
CCACCACCAT TTCCACAGCA GAGAATCGCC AACTCTTTTC CACAACAGCA GGCACTCCAT 
CAACCTCTCC AACAGCAACC GCCTCCTAAT TTCTTGCAGC AACAGCCGTC CTTCTCGTCT 
CCGCCGCAAG CGAGGAGTCC GTTGCAAAGA CCTCAACGAT TGCCTATAAC GATTCAAGGT 
CTACCAGATG TACAACAACG AGCGCCGTTC AATACTTTCG GTACATCTCA TCGCGTCAAC 
ATTAACCACC CACAACAACC AGGGTTCCCA CCTACGTTGT CGGTTTCCCA TCAAAACGTG 
CAGGTACAAA ATCAACCTTT ACCACCGAAC CAGGGCAGTC CTTTCAACGT GGAACAACAG 
CCGCAGCAGC CACCTTTGTC CTCGTATAAA CAACAAGAAT TAGAAAAACA AAGGTATCAC 
GAGCAACGTC GTCAACAGGA ATTGCTAAAG CATCGCGAAC ATCAGAGGCA ACACGAGCAA 
CAAAGACTTC AGGAGCATCA ACGATTCTTG GAACAACAGA GGATACTGGA TCAGCAGAGG 
CAGCACGAAC AACAGAGACA ACAGGATCAT CAAAGATTGC AGGAGCAACA GAGAATACAG 
GAGCAGCAGA GGATACAGGA ACAGCAGAGG ATACAGGAAC AGCAGAGGAT ACAGGAACAG 
CAGAGGATAC AGGAGCAACA GAGATTACAA GACCAACAGA GATTGCAGGA ACAGCAAAGA 
TTACAGGAGC AACAAAGGCT GCAGGAACAA CGCAGAAGGA AACAAGAACA AGAACAGAAA 
TTGTTACAGG AGCAACAGTA TCGTTCGCAA TTGAAATATA ATCAAGCGCA ACCGGTGCAC 
AATATACCGC AAGTACCAAT CTTGCCTCAA ATTCAAAAAC CAGGAGGAGA AATTTTCAAA 
GCTGTTCCGA AGCTCGAGCA ACACTATGCG ATTCGAGAAA ATCCACTTCA TCCTGGCCCT 
TTCCCACCTA ATCCTGTAAT CCAGCCAACT GGATTCCCGG ACACGCCACA GAATCAATAC 
GTTTCCGCTC AACCGCCGAG TTCGTCGCCA GACATCTTAA GAAACCCACC GAGCGAATTT 
ATAAGCGATC AACAGCAACA TCTGAATACG AAACAGCAAA TTATTCAAAG TCAGCAACAA 
GGATTTTTCT CTCAAAATCT TCAACAAGCG CAACAGCAGC AAAGATTCCC AGCGTCTCCA 
CAACGATCTT CGATCTGGGG ACGTCCATCG TTTGCCGGAA ACAGCGCGAG TCCTTCGCAA 
AAGATTTACG CGACTCCAGT CAGTCCCTCT GAAGAATTTA ATTCGATCTC AACCATCAGA 
CCGTTCGTTC CAAGTACAAC TACTACTACC ACGACGACGA CAACGACGAC TACCACCGAA 
GCTCCAACGA CGACAACAAC TTTCTCTCCA AAGAAAGAGG CTAAAATTAG GGAAAATATC 
GCGAATCTTC CGGACGAGGT ACCGGATGAT ATCAGGGAAC AGCTGCTGAG TTCCGGTATT 
TTGGGTAATG CCGACATACA AATACTGGAT TACGACAAGG TCGGCGACAT ACCGATAGAG 
AAACTACCGC CGGAGGCTCT GGCGAATTTC TACGGTGCCG GAGGCGGTTC CGCGGTAGCT 
GCCAGCGAAC CGGTCCCGTC GATCGTCAAG AAACCCCAAA CGGCAGAGAG CGCTTCCTCC 
TCGACCTCCT CTTCTTCCTC CACTTCCTCG CCAGTTTCGG CGAAGAAGGC GGCTACCGAT 
AGCTCGACTA AGTTCGAGCA GGCTACCTTG CGGCCAGGTG GAGTGGAGAT GAAAGTAGTA 
CGCTTCGATC CAAATACGGA GCAAGGCCAG GCGCTAGCGG ATCAACACAT ACGCGATGAT 
GCTACCAGGT TGAATCCCGT AACCGTAGGA GAGAAAGACG AGTCCCAATA TAATCGTTAT 
CTACCGCTCA AAGTGAGTGG TGCTTCCTTC CCTATTCCGG ACGTGCCGCA ACTTAATGGT 
CGGAAGATCT CTAGCGTAGT TGTATTGGCA CCCGTCGACT ATAACTTTCA TGAGGAAAAA 
GAGATTGAAT CGAGGCAAGG CAGAAGGGTC AGCGATGTGC AAGCGGTCAA GTTTCTCGCC 
GGCGATACTC TGAAACAACT GGTGAAAAAA CCAACGGCGG ACAACTACAA GAAGTGGTTG 
GAGCAGGAAA GCCGAACGGA GCCGCAGAAA CAGTCGGTGG TTCTATTGGT AACCATGCCA 
GAGGGCGCGG ATCAAGGCGA GAAGGAAATC TTCATGTATG ATGTGACCTC GCAAACTGTG 
AGCAAACTGG CCGGAGATCT ATCTACAGCG TTTGTAGATG CTGCTGAAAG TAACTCCGAC 
AATACCGACT CTTTCACCGA TTCAACTCTT TCATCGTTTT AA