LOC100741403 cDNA ORF clone, Bombus impatiens(common eastern bumble bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100741403
Entrez Gene ID	100741403
Full Name	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
Gene Type	protein-coding
Organism	Bombus impatiens(common eastern bumble bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012392353.1	XP_012247776.1	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
XM_012392353.2	XP_012247776.1	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16

Pathways from BioSystems
Gene Ontology

The following LOC100741403 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100741403 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBo110654	XM_012392353.2 Latest version!	Bombus impatiens pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 (LOC100741403), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OBo110654	XM_012392353.1	Bombus impatiens putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 (LOC100741403), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100741403 ( XM_012392353.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012247776.1 Bombus impatiens putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 (LOC100741403), mRNA.

Price & Availability↑

CloneID	OBo110654
Clone ID Related Accession (Same CDS sequence)	XM_012392353.1 , XM_012392353.2
Accession Version	XM_012392353.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_177768.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAAAT ACAGAAAAAA GGAATCCTCG AGTGAGGACA GTGATAGTGA GGAGGAAAGA 
CGTAAGAAAG ATATTAAAGA AAGAGATGAG TTCGCTAATC GACTGAAAGC AAAGGATGAA 
AGTAAAATAC GAAAGGTGGC TATGCCAGCC GGTTCAGGTG CAGCTGAGGC TGCCAAGAGA 
CTCAAAATTA TGGAAACAGA CATGCGAGAG AAATTGGTAC CCAAACTGCG TGTTGAATCT 
CGTAGGAAAT ACTTGGAGAA ACGTAAAGAG GATAAGGTGG CTGAATTGGA AGCTGATATA 
ATTGACGATG AATATCTGTT TGAGGAGGAA ATATTAACAG AACGGGAAAG GAAAGAGCGG 
GAGCATAAAA AGCAGTTGCT TCAGTTAGCC AAAGAACATG AGAAAGCCAG AGAACTTGAA 
AGGTTGCAAC GTTACTATAT GCCTTTGGAA AAGGGGAAAG TGGCACCAGA ATTGGAAGTT 
CCCGATAACG AACCGCCACA ATCCGAGCAA AGCAAATGGG AATCTGATCA GATGTCTTCT 
GCTGTCTTTC GATTTGGCGC AAAAGACAGA AAAGCACAAC AGGACTACGA TCTTCTGCTG 
GAAGATGAAG TGGAGTTCGT TCAAGCGTTA CGTATGCCTG GCAGTGAGAA GAACAGGAAA 
CGCGAGGATA GTCCTCCTCC ACAAGTAAAG GCGTTACAGA CTATACAGGA GACAAAAAGG 
AGTTTACCGA TCTATCCCTT TAGAAATGAT CTCATTCAGG CTATCAAAGA CCATCAAGTG 
CTGATAATCG AGGGAGAAAC GGGTTCAGGG AAAACTACGC AAATTCCTCA ATATCTATAC 
GAAGCTGGAT TCGCGGAGAA TAATAAAATT ATTGGCTGTA CGCAGCCAAG AAGGGTAGCT 
GCCATGTCTG TTGCTGCGAG AGTTGCTCAC GAAATGGCTG TTAAATTGGG AAATGAAGTG 
GGTTACGCGA TTCGTTTCGA GGATTGCACC TCTCATCGTA CTCGTATCAA ATATATGACC 
GATGGTACCC TGCACCGTGA ATTTCTCAGC GAACCAGATT TGGCTTCTTA CAGTGTTATG 
ATCATCGACG AAGCACACGA ACGTACCTTG CACACGGATA TCTTGTTTGG TTTAGTAAAG 
GATATAACAA GGTTTCGAAC AGATCTAAAA CTCCTAATTT CGTCCGCTAC ATTAGACGCT 
ACGAAATTTA GTGAATTCTT CGACGATGCA CCGATATTTC GGATACCTGG TCGCCGGTTT 
CCCGTTGATA TTTATTATAC CAAAGCACCA GAAGCGGATT ACATCGACGC CTGCGTAGTC 
TCCATTCTTC AAATACACGC CACTCAGCCA CCTGGCGATG TACTGGTGTT CCTAACAGGT 
CAAGACGAGA TAGAAACATG TCAGGAAATG TTGCAAGAAA GAGTAAGACG ACTAGGTTCG 
AAATTAGGAG AACTGTTAAT CCTACCCGTA TATGCTAATC TTCCAAGCGA TATGCAAGCA 
AAAATATTTC AACCCACTCC GCTTGGTGCG AGAAAAGTAG TTCTTGCTAC GAACATAGCA 
GAGACATCAT TAACGATAGA CAACATAGTG TACGTGATAG ACCCTGGATT CGCGAAACAG 
AACAATTTTA ATTCCCGAAC AGGCATGGAA AGTTTAATGG TCGTGCCGAT TAGCAAAGCT 
TCTGCGAATC AAAGAGCAGG CAGAGCTGGA AGAGTAGCTC CAGGAAAGTG CTTTCGACTG 
TACACAGCTT GGGCTTACCA ACACGAACTC GAAGACAATA CCGTGCCTGA AATTCAGAGG 
ATCAATCTAG GAAATGCCGT GCTGACCCTG AAAGCTCTGG GAATAAACGA TTTAGTCCAT 
TTCGACTTCC TGGATCCTCC GCCGCATGAA ACGTTGGTCT TAGCTTTAGA ACAGCTATAC 
GCGTTGGGAG CTTTGAATCA TCGCGGAGAA TTAACGAAAC TGGGTCGAAG AATGGCTGAA 
TTTCCGCTAG ATCCTATGAT GGCGAAGATG TTGCTGGCTA GCGAACAATA TCGCTGCTCG 
GAGGAAGTAG CCACGATAGC TGCCATGTTA TCTGTGAACG GAGCCATATT CTACAGGCCT 
AAGGACAAGA TCATTCACGC AGACACGGCT CGAAAGAATT TCCATGTGCC TGGTGGCGAT 
CATTTAACTT TATTGAACGT GTACAATCAG TGGCAGCAAA GTGATTTTAG TACTCATTGG 
TGTTATGAGA ATTTCATTCA ACATCGATCA ATGAAAAGAG CTAGAGATGT TAGGGAGCAA 
TTAGTCGGAT TGATGCAGCG AGTCGAGATG GAACTTGTGT CGGGTATCAC GGAAACGCTG 
AATATCAGGA AGGCTATCAC AGCAGGATAT TTCTATCACG TGGCAAGATT GTCAAAAGGA 
GGTCACTATA AAACTGCAAA ACATAATCAG ACGGTTTCCA TTCACCCTAA TAGCTCGTTA 
TTTCAAGAGC TGCCACGTTG GTTACTTTAT CATGAGTTGG TTTTCACTAC AAAGGAATTT 
ATGAGACAAG TGACAGAAAT CGAAAGCAAG TGGCTTTTGG AGGTAGCACC TCATTATTAC 
AAACCAAAAG AACTAGAAGA TTCTACGAAT AAAAAAATGC CGAAAGTTGC AGGACGATCG 
CACCCAGACG GAACCTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012247776.1
CDS	432..3092
Translation	MSKYRKKESSSEDSDSEEERRKKDIKERDEFANRLKAKDESKIRKVAMPAGSGAAEAAKRLKIMETDMREKLVPKLRVESRRKYLEKRKEDKVAELEADIIDDEYLFEEEILTERERKEREHKKQLLQLAKEHEKARELERLQRYYMPLEKGKVAPELEVPDNEPPQSEQSKWESDQMSSAVFRFGAKDRKAQQDYDLLLEDEVEFVQALRMPGSEKNRKREDSPPPQVKALQTIQETKRSLPIYPFRNDLIQAIKDHQVLIIEGETGSGKTTQIPQYLYEAGFAENNKIIGCTQPRRVAAMSVAARVAHEMAVKLGNEVGYAIRFEDCTSHRTRIKYMTDGTLHREFLSEPDLASYSVMIIDEAHERTLHTDILFGLVKDITRFRTDLKLLISSATLDATKFSEFFDDAPIFRIPGRRFPVDIYYTKAPEADYIDACVVSILQIHATQPPGDVLVFLTGQDEIETCQEMLQERVRRLGSKLGELLILPVYANLPSDMQAKIFQPTPLGARKVVLATNIAETSLTIDNIVYVIDPGFAKQNNFNSRTGMESLMVVPISKASANQRAGRAGRVAPGKCFRLYTAWAYQHELEDNTVPEIQRINLGNAVLTLKALGINDLVHFDFLDPPPHETLVLALEQLYALGALNHRGELTKLGRRMAEFPLDPMMAKMLLASEQYRCSEEVATIAAMLSVNGAIFYRPKDKIIHADTARKNFHVPGGDHLTLLNVYNQWQQSDFSTHWCYENFIQHRSMKRARDVREQLVGLMQRVEMELVSGITETLNIRKAITAGYFYHVARLSKGGHYKTAKHNQTVSIHPNSSLFQELPRWLLYHELVFTTKEFMRQVTEIESKWLLEVAPHYYKPKELEDSTNKKMPKVAGRSHPDGTY

Target ORF information:

RefSeq Version	XM_012392353.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 (LOC100741403), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012392353.1

ORF Insert Sequence:

ATGAGCAAAT ACAGAAAAAA GGAATCCTCG AGTGAGGACA GTGATAGTGA GGAGGAAAGA 
CGTAAGAAAG ATATTAAAGA AAGAGATGAG TTCGCTAATC GACTGAAAGC AAAGGATGAA 
AGTAAAATAC GAAAGGTGGC TATGCCAGCC GGTTCAGGTG CAGCTGAGGC TGCCAAGAGA 
CTCAAAATTA TGGAAACAGA CATGCGAGAG AAATTGGTAC CCAAACTGCG TGTTGAATCT 
CGTAGGAAAT ACTTGGAGAA ACGTAAAGAG GATAAGGTGG CTGAATTGGA AGCTGATATA 
ATTGACGATG AATATCTGTT TGAGGAGGAA ATATTAACAG AACGGGAAAG GAAAGAGCGG 
GAGCATAAAA AGCAGTTGCT TCAGTTAGCC AAAGAACATG AGAAAGCCAG AGAACTTGAA 
AGGTTGCAAC GTTACTATAT GCCTTTGGAA AAGGGGAAAG TGGCACCAGA ATTGGAAGTT 
CCCGATAACG AACCGCCACA ATCCGAGCAA AGCAAATGGG AATCTGATCA GATGTCTTCT 
GCTGTCTTTC GATTTGGCGC AAAAGACAGA AAAGCACAAC AGGACTACGA TCTTCTGCTG 
GAAGATGAAG TGGAGTTCGT TCAAGCGTTA CGTATGCCTG GCAGTGAGAA GAACAGGAAA 
CGCGAGGATA GTCCTCCTCC ACAAGTAAAG GCGTTACAGA CTATACAGGA GACAAAAAGG 
AGTTTACCGA TCTATCCCTT TAGAAATGAT CTCATTCAGG CTATCAAAGA CCATCAAGTG 
CTGATAATCG AGGGAGAAAC GGGTTCAGGG AAAACTACGC AAATTCCTCA ATATCTATAC 
GAAGCTGGAT TCGCGGAGAA TAATAAAATT ATTGGCTGTA CGCAGCCAAG AAGGGTAGCT 
GCCATGTCTG TTGCTGCGAG AGTTGCTCAC GAAATGGCTG TTAAATTGGG AAATGAAGTG 
GGTTACGCGA TTCGTTTCGA GGATTGCACC TCTCATCGTA CTCGTATCAA ATATATGACC 
GATGGTACCC TGCACCGTGA ATTTCTCAGC GAACCAGATT TGGCTTCTTA CAGTGTTATG 
ATCATCGACG AAGCACACGA ACGTACCTTG CACACGGATA TCTTGTTTGG TTTAGTAAAG 
GATATAACAA GGTTTCGAAC AGATCTAAAA CTCCTAATTT CGTCCGCTAC ATTAGACGCT 
ACGAAATTTA GTGAATTCTT CGACGATGCA CCGATATTTC GGATACCTGG TCGCCGGTTT 
CCCGTTGATA TTTATTATAC CAAAGCACCA GAAGCGGATT ACATCGACGC CTGCGTAGTC 
TCCATTCTTC AAATACACGC CACTCAGCCA CCTGGCGATG TACTGGTGTT CCTAACAGGT 
CAAGACGAGA TAGAAACATG TCAGGAAATG TTGCAAGAAA GAGTAAGACG ACTAGGTTCG 
AAATTAGGAG AACTGTTAAT CCTACCCGTA TATGCTAATC TTCCAAGCGA TATGCAAGCA 
AAAATATTTC AACCCACTCC GCTTGGTGCG AGAAAAGTAG TTCTTGCTAC GAACATAGCA 
GAGACATCAT TAACGATAGA CAACATAGTG TACGTGATAG ACCCTGGATT CGCGAAACAG 
AACAATTTTA ATTCCCGAAC AGGCATGGAA AGTTTAATGG TCGTGCCGAT TAGCAAAGCT 
TCTGCGAATC AAAGAGCAGG CAGAGCTGGA AGAGTAGCTC CAGGAAAGTG CTTTCGACTG 
TACACAGCTT GGGCTTACCA ACACGAACTC GAAGACAATA CCGTGCCTGA AATTCAGAGG 
ATCAATCTAG GAAATGCCGT GCTGACCCTG AAAGCTCTGG GAATAAACGA TTTAGTCCAT 
TTCGACTTCC TGGATCCTCC GCCGCATGAA ACGTTGGTCT TAGCTTTAGA ACAGCTATAC 
GCGTTGGGAG CTTTGAATCA TCGCGGAGAA TTAACGAAAC TGGGTCGAAG AATGGCTGAA 
TTTCCGCTAG ATCCTATGAT GGCGAAGATG TTGCTGGCTA GCGAACAATA TCGCTGCTCG 
GAGGAAGTAG CCACGATAGC TGCCATGTTA TCTGTGAACG GAGCCATATT CTACAGGCCT 
AAGGACAAGA TCATTCACGC AGACACGGCT CGAAAGAATT TCCATGTGCC TGGTGGCGAT 
CATTTAACTT TATTGAACGT GTACAATCAG TGGCAGCAAA GTGATTTTAG TACTCATTGG 
TGTTATGAGA ATTTCATTCA ACATCGATCA ATGAAAAGAG CTAGAGATGT TAGGGAGCAA 
TTAGTCGGAT TGATGCAGCG AGTCGAGATG GAACTTGTGT CGGGTATCAC GGAAACGCTG 
AATATCAGGA AGGCTATCAC AGCAGGATAT TTCTATCACG TGGCAAGATT GTCAAAAGGA 
GGTCACTATA AAACTGCAAA ACATAATCAG ACGGTTTCCA TTCACCCTAA TAGCTCGTTA 
TTTCAAGAGC TGCCACGTTG GTTACTTTAT CATGAGTTGG TTTTCACTAC AAAGGAATTT 
ATGAGACAAG TGACAGAAAT CGAAAGCAAG TGGCTTTTGG AGGTAGCACC TCATTATTAC 
AAACCAAAAG AACTAGAAGA TTCTACGAAT AAAAAAATGC CGAAAGTTGC AGGACGATCG 
CACCCAGACG GAACCTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100741403 ( XM_012392353.2 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012247776.1 Bombus impatiens pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 (LOC100741403), mRNA.

Price & Availability↑

CloneID	OBo110654
Clone ID Related Accession (Same CDS sequence)	XM_012392353.1 , XM_012392353.2
Accession Version	XM_012392353.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_177768.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 19, 2018 this sequence version replaced XM_012392353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAAAT ACAGAAAAAA GGAATCCTCG AGTGAGGACA GTGATAGTGA GGAGGAAAGA 
CGTAAGAAAG ATATTAAAGA AAGAGATGAG TTCGCTAATC GACTGAAAGC AAAGGATGAA 
AGTAAAATAC GAAAGGTGGC TATGCCAGCC GGTTCAGGTG CAGCTGAGGC TGCCAAGAGA 
CTCAAAATTA TGGAAACAGA CATGCGAGAG AAATTGGTAC CCAAACTGCG TGTTGAATCT 
CGTAGGAAAT ACTTGGAGAA ACGTAAAGAG GATAAGGTGG CTGAATTGGA AGCTGATATA 
ATTGACGATG AATATCTGTT TGAGGAGGAA ATATTAACAG AACGGGAAAG GAAAGAGCGG 
GAGCATAAAA AGCAGTTGCT TCAGTTAGCC AAAGAACATG AGAAAGCCAG AGAACTTGAA 
AGGTTGCAAC GTTACTATAT GCCTTTGGAA AAGGGGAAAG TGGCACCAGA ATTGGAAGTT 
CCCGATAACG AACCGCCACA ATCCGAGCAA AGCAAATGGG AATCTGATCA GATGTCTTCT 
GCTGTCTTTC GATTTGGCGC AAAAGACAGA AAAGCACAAC AGGACTACGA TCTTCTGCTG 
GAAGATGAAG TGGAGTTCGT TCAAGCGTTA CGTATGCCTG GCAGTGAGAA GAACAGGAAA 
CGCGAGGATA GTCCTCCTCC ACAAGTAAAG GCGTTACAGA CTATACAGGA GACAAAAAGG 
AGTTTACCGA TCTATCCCTT TAGAAATGAT CTCATTCAGG CTATCAAAGA CCATCAAGTG 
CTGATAATCG AGGGAGAAAC GGGTTCAGGG AAAACTACGC AAATTCCTCA ATATCTATAC 
GAAGCTGGAT TCGCGGAGAA TAATAAAATT ATTGGCTGTA CGCAGCCAAG AAGGGTAGCT 
GCCATGTCTG TTGCTGCGAG AGTTGCTCAC GAAATGGCTG TTAAATTGGG AAATGAAGTG 
GGTTACGCGA TTCGTTTCGA GGATTGCACC TCTCATCGTA CTCGTATCAA ATATATGACC 
GATGGTACCC TGCACCGTGA ATTTCTCAGC GAACCAGATT TGGCTTCTTA CAGTGTTATG 
ATCATCGACG AAGCACACGA ACGTACCTTG CACACGGATA TCTTGTTTGG TTTAGTAAAG 
GATATAACAA GGTTTCGAAC AGATCTAAAA CTCCTAATTT CGTCCGCTAC ATTAGACGCT 
ACGAAATTTA GTGAATTCTT CGACGATGCA CCGATATTTC GGATACCTGG TCGCCGGTTT 
CCCGTTGATA TTTATTATAC CAAAGCACCA GAAGCGGATT ACATCGACGC CTGCGTAGTC 
TCCATTCTTC AAATACACGC CACTCAGCCA CCTGGCGATG TACTGGTGTT CCTAACAGGT 
CAAGACGAGA TAGAAACATG TCAGGAAATG TTGCAAGAAA GAGTAAGACG ACTAGGTTCG 
AAATTAGGAG AACTGTTAAT CCTACCCGTA TATGCTAATC TTCCAAGCGA TATGCAAGCA 
AAAATATTTC AACCCACTCC GCTTGGTGCG AGAAAAGTAG TTCTTGCTAC GAACATAGCA 
GAGACATCAT TAACGATAGA CAACATAGTG TACGTGATAG ACCCTGGATT CGCGAAACAG 
AACAATTTTA ATTCCCGAAC AGGCATGGAA AGTTTAATGG TCGTGCCGAT TAGCAAAGCT 
TCTGCGAATC AAAGAGCAGG CAGAGCTGGA AGAGTAGCTC CAGGAAAGTG CTTTCGACTG 
TACACAGCTT GGGCTTACCA ACACGAACTC GAAGACAATA CCGTGCCTGA AATTCAGAGG 
ATCAATCTAG GAAATGCCGT GCTGACCCTG AAAGCTCTGG GAATAAACGA TTTAGTCCAT 
TTCGACTTCC TGGATCCTCC GCCGCATGAA ACGTTGGTCT TAGCTTTAGA ACAGCTATAC 
GCGTTGGGAG CTTTGAATCA TCGCGGAGAA TTAACGAAAC TGGGTCGAAG AATGGCTGAA 
TTTCCGCTAG ATCCTATGAT GGCGAAGATG TTGCTGGCTA GCGAACAATA TCGCTGCTCG 
GAGGAAGTAG CCACGATAGC TGCCATGTTA TCTGTGAACG GAGCCATATT CTACAGGCCT 
AAGGACAAGA TCATTCACGC AGACACGGCT CGAAAGAATT TCCATGTGCC TGGTGGCGAT 
CATTTAACTT TATTGAACGT GTACAATCAG TGGCAGCAAA GTGATTTTAG TACTCATTGG 
TGTTATGAGA ATTTCATTCA ACATCGATCA ATGAAAAGAG CTAGAGATGT TAGGGAGCAA 
TTAGTCGGAT TGATGCAGCG AGTCGAGATG GAACTTGTGT CGGGTATCAC GGAAACGCTG 
AATATCAGGA AGGCTATCAC AGCAGGATAT TTCTATCACG TGGCAAGATT GTCAAAAGGA 
GGTCACTATA AAACTGCAAA ACATAATCAG ACGGTTTCCA TTCACCCTAA TAGCTCGTTA 
TTTCAAGAGC TGCCACGTTG GTTACTTTAT CATGAGTTGG TTTTCACTAC AAAGGAATTT 
ATGAGACAAG TGACAGAAAT CGAAAGCAAG TGGCTTTTGG AGGTAGCACC TCATTATTAC 
AAACCAAAAG AACTAGAAGA TTCTACGAAT AAAAAAATGC CGAAAGTTGC AGGACGATCG 
CACCCAGACG GAACCTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012247776.1
CDS	534..3194
Translation	MSKYRKKESSSEDSDSEEERRKKDIKERDEFANRLKAKDESKIRKVAMPAGSGAAEAAKRLKIMETDMREKLVPKLRVESRRKYLEKRKEDKVAELEADIIDDEYLFEEEILTERERKEREHKKQLLQLAKEHEKARELERLQRYYMPLEKGKVAPELEVPDNEPPQSEQSKWESDQMSSAVFRFGAKDRKAQQDYDLLLEDEVEFVQALRMPGSEKNRKREDSPPPQVKALQTIQETKRSLPIYPFRNDLIQAIKDHQVLIIEGETGSGKTTQIPQYLYEAGFAENNKIIGCTQPRRVAAMSVAARVAHEMAVKLGNEVGYAIRFEDCTSHRTRIKYMTDGTLHREFLSEPDLASYSVMIIDEAHERTLHTDILFGLVKDITRFRTDLKLLISSATLDATKFSEFFDDAPIFRIPGRRFPVDIYYTKAPEADYIDACVVSILQIHATQPPGDVLVFLTGQDEIETCQEMLQERVRRLGSKLGELLILPVYANLPSDMQAKIFQPTPLGARKVVLATNIAETSLTIDNIVYVIDPGFAKQNNFNSRTGMESLMVVPISKASANQRAGRAGRVAPGKCFRLYTAWAYQHELEDNTVPEIQRINLGNAVLTLKALGINDLVHFDFLDPPPHETLVLALEQLYALGALNHRGELTKLGRRMAEFPLDPMMAKMLLASEQYRCSEEVATIAAMLSVNGAIFYRPKDKIIHADTARKNFHVPGGDHLTLLNVYNQWQQSDFSTHWCYENFIQHRSMKRARDVREQLVGLMQRVEMELVSGITETLNIRKAITAGYFYHVARLSKGGHYKTAKHNQTVSIHPNSSLFQELPRWLLYHELVFTTKEFMRQVTEIESKWLLEVAPHYYKPKELEDSTNKKMPKVAGRSHPDGTY

Target ORF information:

RefSeq Version	XM_012392353.2
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 (LOC100741403), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012392353.2

ORF Insert Sequence:

ATGAGCAAAT ACAGAAAAAA GGAATCCTCG AGTGAGGACA GTGATAGTGA GGAGGAAAGA 
CGTAAGAAAG ATATTAAAGA AAGAGATGAG TTCGCTAATC GACTGAAAGC AAAGGATGAA 
AGTAAAATAC GAAAGGTGGC TATGCCAGCC GGTTCAGGTG CAGCTGAGGC TGCCAAGAGA 
CTCAAAATTA TGGAAACAGA CATGCGAGAG AAATTGGTAC CCAAACTGCG TGTTGAATCT 
CGTAGGAAAT ACTTGGAGAA ACGTAAAGAG GATAAGGTGG CTGAATTGGA AGCTGATATA 
ATTGACGATG AATATCTGTT TGAGGAGGAA ATATTAACAG AACGGGAAAG GAAAGAGCGG 
GAGCATAAAA AGCAGTTGCT TCAGTTAGCC AAAGAACATG AGAAAGCCAG AGAACTTGAA 
AGGTTGCAAC GTTACTATAT GCCTTTGGAA AAGGGGAAAG TGGCACCAGA ATTGGAAGTT 
CCCGATAACG AACCGCCACA ATCCGAGCAA AGCAAATGGG AATCTGATCA GATGTCTTCT 
GCTGTCTTTC GATTTGGCGC AAAAGACAGA AAAGCACAAC AGGACTACGA TCTTCTGCTG 
GAAGATGAAG TGGAGTTCGT TCAAGCGTTA CGTATGCCTG GCAGTGAGAA GAACAGGAAA 
CGCGAGGATA GTCCTCCTCC ACAAGTAAAG GCGTTACAGA CTATACAGGA GACAAAAAGG 
AGTTTACCGA TCTATCCCTT TAGAAATGAT CTCATTCAGG CTATCAAAGA CCATCAAGTG 
CTGATAATCG AGGGAGAAAC GGGTTCAGGG AAAACTACGC AAATTCCTCA ATATCTATAC 
GAAGCTGGAT TCGCGGAGAA TAATAAAATT ATTGGCTGTA CGCAGCCAAG AAGGGTAGCT 
GCCATGTCTG TTGCTGCGAG AGTTGCTCAC GAAATGGCTG TTAAATTGGG AAATGAAGTG 
GGTTACGCGA TTCGTTTCGA GGATTGCACC TCTCATCGTA CTCGTATCAA ATATATGACC 
GATGGTACCC TGCACCGTGA ATTTCTCAGC GAACCAGATT TGGCTTCTTA CAGTGTTATG 
ATCATCGACG AAGCACACGA ACGTACCTTG CACACGGATA TCTTGTTTGG TTTAGTAAAG 
GATATAACAA GGTTTCGAAC AGATCTAAAA CTCCTAATTT CGTCCGCTAC ATTAGACGCT 
ACGAAATTTA GTGAATTCTT CGACGATGCA CCGATATTTC GGATACCTGG TCGCCGGTTT 
CCCGTTGATA TTTATTATAC CAAAGCACCA GAAGCGGATT ACATCGACGC CTGCGTAGTC 
TCCATTCTTC AAATACACGC CACTCAGCCA CCTGGCGATG TACTGGTGTT CCTAACAGGT 
CAAGACGAGA TAGAAACATG TCAGGAAATG TTGCAAGAAA GAGTAAGACG ACTAGGTTCG 
AAATTAGGAG AACTGTTAAT CCTACCCGTA TATGCTAATC TTCCAAGCGA TATGCAAGCA 
AAAATATTTC AACCCACTCC GCTTGGTGCG AGAAAAGTAG TTCTTGCTAC GAACATAGCA 
GAGACATCAT TAACGATAGA CAACATAGTG TACGTGATAG ACCCTGGATT CGCGAAACAG 
AACAATTTTA ATTCCCGAAC AGGCATGGAA AGTTTAATGG TCGTGCCGAT TAGCAAAGCT 
TCTGCGAATC AAAGAGCAGG CAGAGCTGGA AGAGTAGCTC CAGGAAAGTG CTTTCGACTG 
TACACAGCTT GGGCTTACCA ACACGAACTC GAAGACAATA CCGTGCCTGA AATTCAGAGG 
ATCAATCTAG GAAATGCCGT GCTGACCCTG AAAGCTCTGG GAATAAACGA TTTAGTCCAT 
TTCGACTTCC TGGATCCTCC GCCGCATGAA ACGTTGGTCT TAGCTTTAGA ACAGCTATAC 
GCGTTGGGAG CTTTGAATCA TCGCGGAGAA TTAACGAAAC TGGGTCGAAG AATGGCTGAA 
TTTCCGCTAG ATCCTATGAT GGCGAAGATG TTGCTGGCTA GCGAACAATA TCGCTGCTCG 
GAGGAAGTAG CCACGATAGC TGCCATGTTA TCTGTGAACG GAGCCATATT CTACAGGCCT 
AAGGACAAGA TCATTCACGC AGACACGGCT CGAAAGAATT TCCATGTGCC TGGTGGCGAT 
CATTTAACTT TATTGAACGT GTACAATCAG TGGCAGCAAA GTGATTTTAG TACTCATTGG 
TGTTATGAGA ATTTCATTCA ACATCGATCA ATGAAAAGAG CTAGAGATGT TAGGGAGCAA 
TTAGTCGGAT TGATGCAGCG AGTCGAGATG GAACTTGTGT CGGGTATCAC GGAAACGCTG 
AATATCAGGA AGGCTATCAC AGCAGGATAT TTCTATCACG TGGCAAGATT GTCAAAAGGA 
GGTCACTATA AAACTGCAAA ACATAATCAG ACGGTTTCCA TTCACCCTAA TAGCTCGTTA 
TTTCAAGAGC TGCCACGTTG GTTACTTTAT CATGAGTTGG TTTTCACTAC AAAGGAATTT 
ATGAGACAAG TGACAGAAAT CGAAAGCAAG TGGCTTTTGG AGGTAGCACC TCATTATTAC 
AAACCAAAAG AACTAGAAGA TTCTACGAAT AAAAAAATGC CGAAAGTTGC AGGACGATCG 
CACCCAGACG GAACCTACTA A