LOC110170500 cDNA ORF clone, Boleophthalmus pectinirostris(great blue-spotted mudskipper)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110170500
Entrez Gene ID	110170500
Full Name	regulator of nonsense transcripts 1-like
Gene Type	protein-coding
Organism	Boleophthalmus pectinirostris(great blue-spotted mudskipper)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020936154.1	XP_020791813.1	regulator of nonsense transcripts 1-like isoform X1
XM_020936155.1	XP_020791814.1	regulator of nonsense transcripts 1-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC110170500 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110170500 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo80860	XM_020936154.1 Latest version!	Boleophthalmus pectinirostris regulator of nonsense transcripts 1-like (LOC110170500), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBo80861	XM_020936155.1 Latest version!	Boleophthalmus pectinirostris regulator of nonsense transcripts 1-like (LOC110170500), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110170500 ( XM_020936154.1 ) cDNA ORF clone, Boleophthalmus pectinirostris(great blue-spotted mudskipper) -> XP_020791813.1 Boleophthalmus pectinirostris regulator of nonsense transcripts 1-like (LOC110170500), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBo80860
Clone ID Related Accession (Same CDS sequence)	XM_020936154.1
Accession Version	XM_020936154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-28
Organism	Boleophthalmus pectinirostris(great blue-spotted mudskipper)
Product	regulator of nonsense transcripts 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018358680.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Boleophthalmus pectinirostris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGG AGGCGTACGG GCCGAGCTCG CAGACGCTCA CCTTCCTGGA CACGGAGGAA 
GCGGAGCTGA TCGGAGCGGA CACGCAGGGC TCCGAGTACG ACTTCACCGA CTTCACCCTG 
CCGAGCCAGA GCCAGGGCCC GGGCCACACG CAGGGCCACA CGCAGGGCCA CGCGCAGGGC 
CAGCTGGACG CGCAGTTGAA CGGTCCAGAT GACGGGCTTC ACAACGGAGG AGTGGACGAC 
ACTGTGGCCA AAACCACTCA GCTGCTGCAG GAGCTCAACT TCGAGGAGGA CGAGGAGGAC 
GCGTACTACA CCAAAGACCT GCCCGTGCAC GCCTGCAGTT ACTGCGGCAT CCACGACCCG 
GCCTGCGTCG TTTACTGCAA CACCAGCAAG AAATGGTTCT GTAACGGCCG AGGAAACACG 
TCCGGGAGCC ACATCGTGAA CCACCTGGTG CGTGCGAAGT GTAAAGAGGT GACTCTGCAC 
AAAGACGGGC CCCTGGGCGA GACGGTGCTC GAGTGCTACA ACTGCGGCTG TAGAAACGTC 
TTCCTGCTCG GCTTCATCCC CGCCAAGGCC GACTCTGTGG TGGTGCTCCT CTGCAGACAG 
CCCTGCGCCA GTCAGAGCTC CCTGAAGGAC ATCAACTGGG ACAGCTCCCA GTGGCAGCCG 
CTCATCCAGG ACCGCTGCTT CCTGTCGTGG CTCGTCAAGA TCCCGTCGGA GCAGGAGCAG 
CTCAGGGCCC GGCAGATCTC GGCGCAGCAG ATCAACAAGC TGGAGGAACT GTGGAAGGAT 
AACCCCGGGG CGTCGCTGGA GGACCTGGAG AAGCCCGGAG TGGACGAAGA GCCTCAGCAG 
GTTCTGCTCA GATACGAAGA CGCGTATCAG TATCAGAACG TGTTCGGACC TCTGGTCAAA 
CACGAGGCCG ACTACGACAA GAAGCTCAAA GAGTCTCAGA CCCAAGACAA CATCACCATC 
CGCTGGGACC TGGGTCTGAA CAAAAAGCGC ATAGCGTACT TTACCCTGCC CAAGACGGAC 
TCAGGTGCTG CCTTCTGTGA CATGAGGCTG ATGCAGGGAG ACGAGATCTG CCTGAGGTAC 
AAAGGAGACC TGGGCGTCCC GTGGAAGGGC ATCGGGCACG TCATCAAAGT CCCCGACAAT 
TATGGAGATG AAATCGCCAT CGAGCTTCGC AGCGGAGCCA ACGCTCCCGT CGACATCACC 
CACAACTTCC AGGTGGACTT TGTCTGGAAG TCCACGTCTT TCGACAGGAT GCAGAGCGCC 
CTGAAGACGT TCGCCGTGGA CGAGACGTCC GTGTCCGGGT ACATTTACCA CAAACTCCTG 
GGGCACGAGG TGGAGGACGT CACCATCAAG TGTCAGCTCC CCAAACGCTT CACCGCCCAG 
GGCCTGCCCG ATCTCAACCA CTCACAGGTG TACGCTGTGA AGACGGTGCT GCAGAGGCCT 
CTGAGTCTGA TCCAGGGACC TCCAGGAACC GGCAAAACTG TCACGTCTGC CACCATCGTG 
TACCACCTGT CGCGCCAGGG CAACGGGCCG GTGCTGGTGT GTGCTCCCAG TAACATCGCC 
GTGGATCAGC TCACAGAAAA GATCGACAAA ACTGGACTGA AGGTGGTGCG TCTGTGCGCC 
AAGAGCAGAG AGGCCATCGA GTCCCCGGTG TCTTTCCTCG CCCTGCACAA CCAGATCAGC 
AACATGGACA GCATGCCCGA GCTGCAGAAG CTCCAGCAGC TGAAGGACGA GACCGGAGAG 
CTGTCCTCCG CTGACGAGAA GAGATACAGA GCTCTGAAGA GGACCGCGGA GAGGGAGCTG 
CTCATGAATG CAGACGTGAT CTGCTGCACC TGCGTCGGAG CCGGAGATCC TCGTTTGGCC 
AAGATGCAGT TTCGCTCGAT TCTGATCGAC GAGAGCACTC AGGCCACAGA GCCCGAGTGC 
ATGGTCCCCG TGGTGCTCGG GGCCAAACAG CTGATCCTGG TGGGCGATCA CTGCCAGCTG 
GGTCCTGTGG TGATGTGTAA GAAGGCGGCC AAAGCGGGTC TGTCCCAGTC TCTGTTCGAG 
CGTCTGGTGG TCCTGGGCAT CAGACCCATC CGGCTCCAGG TGCAGTACCG CATGCACCCC 
GCCCTCAGCG CCTTCCCCTC CAACATCTTC TACGAGGGCT CCCTGCAGAA CGGCGTCACG 
GCAGCCGATC GCATAAAGAA AGGCTTTGAC TTCCAGTGGC CGCAGCCGGA CAAACCCATG 
TTCTTCTACG TGACTCAGGG ACAGGAGGAG ATCGCCAGCT CCGGGACCTC CTACCTCAAC 
AGGACTGAAG CGGCCAACGT GGAGAAGATC ACCACTCGTC TGCTGAAAGC TGGAGCCAAA 
CCGGATCAGA TCGGCATCAT CACCCCGTAC GAGGGACAGC GCTCCTACCT GGTCCAGTAC 
ATGCAGTTCA GCGGCTCCCT GCACACCAAA CTCTACCAGC AGGTGGAGAT AGCGAGCGTG 
GACGCGTTCC AGGGCCGAGA GAAGGACTTC ATCATCCTGT CGTGCGTCCG AGCCAACGAG 
CACCAGGGCA TCGGCTTCCT CAACGATCCG CGCCGCCTCA ACGTCGCCCT CACCAGAGCC 
AAGTACGGCG TGATCATCGT GGGGAACCCC AAGGCCCTGT CGAAGCAGCC GCTGTGGAAC 
AACCTCCTGA ACTACTACAA AGAGCAGAAG GTCCTGGTGG AGGGGCCCCT GAACAACCTG 
AGGGAGAGTC TCATGCAGTT CAGCAAACCC CGAAAACTGC TCAACACCAT CAACCCCGGA 
GGGCGCTTCA TGAGCACAGC CATGTACGAC GCCAGAGAGG CTCTGATCCC CGGCTCCGCC 
TACGACCGCT CCAGCGCACC GGGACGTCCC TCTAACATGT ACTTCCAGAC CCATGATCAG 
ATCGGGATGA TCGGGGCAGG ACCGGGGCAC ATGGCCATGA ACCTGCCCAT CCCCCTGAAC 
CTGGTGATGC CCCCGATGCC CCCGCCCAGT TACATGGGCC ACGCCAACGG CCCCGCAGGG 
ATGAAAGGCG GGATGAAGGG AAAGCCGGGC CGCGGTCGGG GTCAGAGGTC ACGTGGAGGC 
GGGGCTCAGG GGAACGGCCC CCACAGCCAG GCCAGTCAGG ACGGGGCCTC CCAGCCCTTC 
TCCCAGGGGC CCCTCACTCA GGGCTACATG AACATGAGCC AACCCTCTCA GATGAGCCAA 
CCGGGGCTGT CCCAACCCGA GCTCTCGCAG GACAGTTACC TCGGAGACGA GTTCAAGTCC 
CAGATGGACG TGGCCCTGTC CCAGGACTCC ACCTACCAGG GCGAGAGAGC CTATCAGCAC 
GGGGTGACCG GACTGTCCCA GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020791813.1
CDS	198..3524
Translation	MSVEAYGPSSQTLTFLDTEEAELIGADTQGSEYDFTDFTLPSQSQGPGHTQGHTQGHAQGQLDAQLNGPDDGLHNGGVDDTVAKTTQLLQELNFEEDEEDAYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQISAQQINKLEELWKDNPGASLEDLEKPGVDEEPQQVLLRYEDAYQYQNVFGPLVKHEADYDKKLKESQTQDNITIRWDLGLNKKRIAYFTLPKTDSGAAFCDMRLMQGDEICLRYKGDLGVPWKGIGHVIKVPDNYGDEIAIELRSGANAPVDITHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVTIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLSRQGNGPVLVCAPSNIAVDQLTEKIDKTGLKVVRLCAKSREAIESPVSFLALHNQISNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRIKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTRLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQQVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRAKYGVIIVGNPKALSKQPLWNNLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLLNTINPGGRFMSTAMYDAREALIPGSAYDRSSAPGRPSNMYFQTHDQIGMIGAGPGHMAMNLPIPLNLVMPPMPPPSYMGHANGPAGMKGGMKGKPGRGRGQRSRGGGAQGNGPHSQASQDGASQPFSQGPLTQGYMNMSQPSQMSQPGLSQPELSQDSYLGDEFKSQMDVALSQDSTYQGERAYQHGVTGLSQY

Target ORF information:

RefSeq Version	XM_020936154.1
Organism	Boleophthalmus pectinirostris(great blue-spotted mudskipper)
Definition	Boleophthalmus pectinirostris regulator of nonsense transcripts 1-like (LOC110170500), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020936154.1

ORF Insert Sequence:

ATGAGTGTGG AGGCGTACGG GCCGAGCTCG CAGACGCTCA CCTTCCTGGA CACGGAGGAA 
GCGGAGCTGA TCGGAGCGGA CACGCAGGGC TCCGAGTACG ACTTCACCGA CTTCACCCTG 
CCGAGCCAGA GCCAGGGCCC GGGCCACACG CAGGGCCACA CGCAGGGCCA CGCGCAGGGC 
CAGCTGGACG CGCAGTTGAA CGGTCCAGAT GACGGGCTTC ACAACGGAGG AGTGGACGAC 
ACTGTGGCCA AAACCACTCA GCTGCTGCAG GAGCTCAACT TCGAGGAGGA CGAGGAGGAC 
GCGTACTACA CCAAAGACCT GCCCGTGCAC GCCTGCAGTT ACTGCGGCAT CCACGACCCG 
GCCTGCGTCG TTTACTGCAA CACCAGCAAG AAATGGTTCT GTAACGGCCG AGGAAACACG 
TCCGGGAGCC ACATCGTGAA CCACCTGGTG CGTGCGAAGT GTAAAGAGGT GACTCTGCAC 
AAAGACGGGC CCCTGGGCGA GACGGTGCTC GAGTGCTACA ACTGCGGCTG TAGAAACGTC 
TTCCTGCTCG GCTTCATCCC CGCCAAGGCC GACTCTGTGG TGGTGCTCCT CTGCAGACAG 
CCCTGCGCCA GTCAGAGCTC CCTGAAGGAC ATCAACTGGG ACAGCTCCCA GTGGCAGCCG 
CTCATCCAGG ACCGCTGCTT CCTGTCGTGG CTCGTCAAGA TCCCGTCGGA GCAGGAGCAG 
CTCAGGGCCC GGCAGATCTC GGCGCAGCAG ATCAACAAGC TGGAGGAACT GTGGAAGGAT 
AACCCCGGGG CGTCGCTGGA GGACCTGGAG AAGCCCGGAG TGGACGAAGA GCCTCAGCAG 
GTTCTGCTCA GATACGAAGA CGCGTATCAG TATCAGAACG TGTTCGGACC TCTGGTCAAA 
CACGAGGCCG ACTACGACAA GAAGCTCAAA GAGTCTCAGA CCCAAGACAA CATCACCATC 
CGCTGGGACC TGGGTCTGAA CAAAAAGCGC ATAGCGTACT TTACCCTGCC CAAGACGGAC 
TCAGGTGCTG CCTTCTGTGA CATGAGGCTG ATGCAGGGAG ACGAGATCTG CCTGAGGTAC 
AAAGGAGACC TGGGCGTCCC GTGGAAGGGC ATCGGGCACG TCATCAAAGT CCCCGACAAT 
TATGGAGATG AAATCGCCAT CGAGCTTCGC AGCGGAGCCA ACGCTCCCGT CGACATCACC 
CACAACTTCC AGGTGGACTT TGTCTGGAAG TCCACGTCTT TCGACAGGAT GCAGAGCGCC 
CTGAAGACGT TCGCCGTGGA CGAGACGTCC GTGTCCGGGT ACATTTACCA CAAACTCCTG 
GGGCACGAGG TGGAGGACGT CACCATCAAG TGTCAGCTCC CCAAACGCTT CACCGCCCAG 
GGCCTGCCCG ATCTCAACCA CTCACAGGTG TACGCTGTGA AGACGGTGCT GCAGAGGCCT 
CTGAGTCTGA TCCAGGGACC TCCAGGAACC GGCAAAACTG TCACGTCTGC CACCATCGTG 
TACCACCTGT CGCGCCAGGG CAACGGGCCG GTGCTGGTGT GTGCTCCCAG TAACATCGCC 
GTGGATCAGC TCACAGAAAA GATCGACAAA ACTGGACTGA AGGTGGTGCG TCTGTGCGCC 
AAGAGCAGAG AGGCCATCGA GTCCCCGGTG TCTTTCCTCG CCCTGCACAA CCAGATCAGC 
AACATGGACA GCATGCCCGA GCTGCAGAAG CTCCAGCAGC TGAAGGACGA GACCGGAGAG 
CTGTCCTCCG CTGACGAGAA GAGATACAGA GCTCTGAAGA GGACCGCGGA GAGGGAGCTG 
CTCATGAATG CAGACGTGAT CTGCTGCACC TGCGTCGGAG CCGGAGATCC TCGTTTGGCC 
AAGATGCAGT TTCGCTCGAT TCTGATCGAC GAGAGCACTC AGGCCACAGA GCCCGAGTGC 
ATGGTCCCCG TGGTGCTCGG GGCCAAACAG CTGATCCTGG TGGGCGATCA CTGCCAGCTG 
GGTCCTGTGG TGATGTGTAA GAAGGCGGCC AAAGCGGGTC TGTCCCAGTC TCTGTTCGAG 
CGTCTGGTGG TCCTGGGCAT CAGACCCATC CGGCTCCAGG TGCAGTACCG CATGCACCCC 
GCCCTCAGCG CCTTCCCCTC CAACATCTTC TACGAGGGCT CCCTGCAGAA CGGCGTCACG 
GCAGCCGATC GCATAAAGAA AGGCTTTGAC TTCCAGTGGC CGCAGCCGGA CAAACCCATG 
TTCTTCTACG TGACTCAGGG ACAGGAGGAG ATCGCCAGCT CCGGGACCTC CTACCTCAAC 
AGGACTGAAG CGGCCAACGT GGAGAAGATC ACCACTCGTC TGCTGAAAGC TGGAGCCAAA 
CCGGATCAGA TCGGCATCAT CACCCCGTAC GAGGGACAGC GCTCCTACCT GGTCCAGTAC 
ATGCAGTTCA GCGGCTCCCT GCACACCAAA CTCTACCAGC AGGTGGAGAT AGCGAGCGTG 
GACGCGTTCC AGGGCCGAGA GAAGGACTTC ATCATCCTGT CGTGCGTCCG AGCCAACGAG 
CACCAGGGCA TCGGCTTCCT CAACGATCCG CGCCGCCTCA ACGTCGCCCT CACCAGAGCC 
AAGTACGGCG TGATCATCGT GGGGAACCCC AAGGCCCTGT CGAAGCAGCC GCTGTGGAAC 
AACCTCCTGA ACTACTACAA AGAGCAGAAG GTCCTGGTGG AGGGGCCCCT GAACAACCTG 
AGGGAGAGTC TCATGCAGTT CAGCAAACCC CGAAAACTGC TCAACACCAT CAACCCCGGA 
GGGCGCTTCA TGAGCACAGC CATGTACGAC GCCAGAGAGG CTCTGATCCC CGGCTCCGCC 
TACGACCGCT CCAGCGCACC GGGACGTCCC TCTAACATGT ACTTCCAGAC CCATGATCAG 
ATCGGGATGA TCGGGGCAGG ACCGGGGCAC ATGGCCATGA ACCTGCCCAT CCCCCTGAAC 
CTGGTGATGC CCCCGATGCC CCCGCCCAGT TACATGGGCC ACGCCAACGG CCCCGCAGGG 
ATGAAAGGCG GGATGAAGGG AAAGCCGGGC CGCGGTCGGG GTCAGAGGTC ACGTGGAGGC 
GGGGCTCAGG GGAACGGCCC CCACAGCCAG GCCAGTCAGG ACGGGGCCTC CCAGCCCTTC 
TCCCAGGGGC CCCTCACTCA GGGCTACATG AACATGAGCC AACCCTCTCA GATGAGCCAA 
CCGGGGCTGT CCCAACCCGA GCTCTCGCAG GACAGTTACC TCGGAGACGA GTTCAAGTCC 
CAGATGGACG TGGCCCTGTC CCAGGACTCC ACCTACCAGG GCGAGAGAGC CTATCAGCAC 
GGGGTGACCG GACTGTCCCA GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110170500 ( XM_020936155.1 ) cDNA ORF clone, Boleophthalmus pectinirostris(great blue-spotted mudskipper) -> XP_020791814.1 Boleophthalmus pectinirostris regulator of nonsense transcripts 1-like (LOC110170500), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBo80861
Clone ID Related Accession (Same CDS sequence)	XM_020936155.1
Accession Version	XM_020936155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-28
Organism	Boleophthalmus pectinirostris(great blue-spotted mudskipper)
Product	regulator of nonsense transcripts 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018358680.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Boleophthalmus pectinirostris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGG AGGCGTACGG GCCGAGCTCG CAGACGCTCA CCTTCCTGGA CACGGAGGAA 
GCGGAGCTGA TCGGAGCGGA CACGCAGGGC TCCGAGTACG ACTTCACCGA CTTCACCCTG 
CCGAGCCAGA GCCAGGGCCC GGGCCACACG CAGGGCCACA CGCAGGGCCA CGCGCAGGGC 
CAGCTGGACG CGCAGTTGAA CGGTCCAGAT GACGGGCTTC ACAACGGAGG AGTGGACGAC 
ACTGTGGCCA AAACCACTCA GCTGCTGCAG GAGCTCAACT TCGAGGAGGA CGAGGAGGAC 
GCGTACTACA CCAAAGACCT GCCCGTGCAC GCCTGCAGTT ACTGCGGCAT CCACGACCCG 
GCCTGCGTCG TTTACTGCAA CACCAGCAAG AAATGGTTCT GTAACGGCCG AGGAAACACG 
TCCGGGAGCC ACATCGTGAA CCACCTGGTG CGTGCGAAGT GTAAAGAGGT GACTCTGCAC 
AAAGACGGGC CCCTGGGCGA GACGGTGCTC GAGTGCTACA ACTGCGGCTG TAGAAACGTC 
TTCCTGCTCG GCTTCATCCC CGCCAAGGCC GACTCTGTGG TGGTGCTCCT CTGCAGACAG 
CCCTGCGCCA GTCAGAGCTC CCTGAAGGAC ATCAACTGGG ACAGCTCCCA GTGGCAGCCG 
CTCATCCAGG ACCGCTGCTT CCTGTCGTGG CTCGTCAAGA TCCCGTCGGA GCAGGAGCAG 
CTCAGGGCCC GGCAGATCTC GGCGCAGCAG ATCAACAAGC TGGAGGAACT GTGGAAGGAT 
AACCCCGGGG CGTCGCTGGA GGACCTGGAG AAGCCCGGAG TGGACGAAGA GCCTCAGCAG 
GTTCTGCTCA GATACGAAGA CGCGTATCAG TATCAGAACG TGTTCGGACC TCTGGTCAAA 
CACGAGGCCG ACTACGACAA GAAGCTCAAA GAGTCTCAGA CCCAAGACAA CATCACCATC 
CGCTGGGACC TGGGTCTGAA CAAAAAGCGC ATAGCGTACT TTACCCTGCC CAAGACGGAC 
TCAGACATGA GGCTGATGCA GGGAGACGAG ATCTGCCTGA GGTACAAAGG AGACCTGGGC 
GTCCCGTGGA AGGGCATCGG GCACGTCATC AAAGTCCCCG ACAATTATGG AGATGAAATC 
GCCATCGAGC TTCGCAGCGG AGCCAACGCT CCCGTCGACA TCACCCACAA CTTCCAGGTG 
GACTTTGTCT GGAAGTCCAC GTCTTTCGAC AGGATGCAGA GCGCCCTGAA GACGTTCGCC 
GTGGACGAGA CGTCCGTGTC CGGGTACATT TACCACAAAC TCCTGGGGCA CGAGGTGGAG 
GACGTCACCA TCAAGTGTCA GCTCCCCAAA CGCTTCACCG CCCAGGGCCT GCCCGATCTC 
AACCACTCAC AGGTGTACGC TGTGAAGACG GTGCTGCAGA GGCCTCTGAG TCTGATCCAG 
GGACCTCCAG GAACCGGCAA AACTGTCACG TCTGCCACCA TCGTGTACCA CCTGTCGCGC 
CAGGGCAACG GGCCGGTGCT GGTGTGTGCT CCCAGTAACA TCGCCGTGGA TCAGCTCACA 
GAAAAGATCG ACAAAACTGG ACTGAAGGTG GTGCGTCTGT GCGCCAAGAG CAGAGAGGCC 
ATCGAGTCCC CGGTGTCTTT CCTCGCCCTG CACAACCAGA TCAGCAACAT GGACAGCATG 
CCCGAGCTGC AGAAGCTCCA GCAGCTGAAG GACGAGACCG GAGAGCTGTC CTCCGCTGAC 
GAGAAGAGAT ACAGAGCTCT GAAGAGGACC GCGGAGAGGG AGCTGCTCAT GAATGCAGAC 
GTGATCTGCT GCACCTGCGT CGGAGCCGGA GATCCTCGTT TGGCCAAGAT GCAGTTTCGC 
TCGATTCTGA TCGACGAGAG CACTCAGGCC ACAGAGCCCG AGTGCATGGT CCCCGTGGTG 
CTCGGGGCCA AACAGCTGAT CCTGGTGGGC GATCACTGCC AGCTGGGTCC TGTGGTGATG 
TGTAAGAAGG CGGCCAAAGC GGGTCTGTCC CAGTCTCTGT TCGAGCGTCT GGTGGTCCTG 
GGCATCAGAC CCATCCGGCT CCAGGTGCAG TACCGCATGC ACCCCGCCCT CAGCGCCTTC 
CCCTCCAACA TCTTCTACGA GGGCTCCCTG CAGAACGGCG TCACGGCAGC CGATCGCATA 
AAGAAAGGCT TTGACTTCCA GTGGCCGCAG CCGGACAAAC CCATGTTCTT CTACGTGACT 
CAGGGACAGG AGGAGATCGC CAGCTCCGGG ACCTCCTACC TCAACAGGAC TGAAGCGGCC 
AACGTGGAGA AGATCACCAC TCGTCTGCTG AAAGCTGGAG CCAAACCGGA TCAGATCGGC 
ATCATCACCC CGTACGAGGG ACAGCGCTCC TACCTGGTCC AGTACATGCA GTTCAGCGGC 
TCCCTGCACA CCAAACTCTA CCAGCAGGTG GAGATAGCGA GCGTGGACGC GTTCCAGGGC 
CGAGAGAAGG ACTTCATCAT CCTGTCGTGC GTCCGAGCCA ACGAGCACCA GGGCATCGGC 
TTCCTCAACG ATCCGCGCCG CCTCAACGTC GCCCTCACCA GAGCCAAGTA CGGCGTGATC 
ATCGTGGGGA ACCCCAAGGC CCTGTCGAAG CAGCCGCTGT GGAACAACCT CCTGAACTAC 
TACAAAGAGC AGAAGGTCCT GGTGGAGGGG CCCCTGAACA ACCTGAGGGA GAGTCTCATG 
CAGTTCAGCA AACCCCGAAA ACTGCTCAAC ACCATCAACC CCGGAGGGCG CTTCATGAGC 
ACAGCCATGT ACGACGCCAG AGAGGCTCTG ATCCCCGGCT CCGCCTACGA CCGCTCCAGC 
GCACCGGGAC GTCCCTCTAA CATGTACTTC CAGACCCATG ATCAGATCGG GATGATCGGG 
GCAGGACCGG GGCACATGGC CATGAACCTG CCCATCCCCC TGAACCTGGT GATGCCCCCG 
ATGCCCCCGC CCAGTTACAT GGGCCACGCC AACGGCCCCG CAGGGATGAA AGGCGGGATG 
AAGGGAAAGC CGGGCCGCGG TCGGGGTCAG AGGTCACGTG GAGGCGGGGC TCAGGGGAAC 
GGCCCCCACA GCCAGGCCAG TCAGGACGGG GCCTCCCAGC CCTTCTCCCA GGGGCCCCTC 
ACTCAGGGCT ACATGAACAT GAGCCAACCC TCTCAGATGA GCCAACCGGG GCTGTCCCAA 
CCCGAGCTCT CGCAGGACAG TTACCTCGGA GACGAGTTCA AGTCCCAGAT GGACGTGGCC 
CTGTCCCAGG ACTCCACCTA CCAGGGCGAG AGAGCCTATC AGCACGGGGT GACCGGACTG 
TCCCAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020791814.1
CDS	198..3509
Translation	MSVEAYGPSSQTLTFLDTEEAELIGADTQGSEYDFTDFTLPSQSQGPGHTQGHTQGHAQGQLDAQLNGPDDGLHNGGVDDTVAKTTQLLQELNFEEDEEDAYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQISAQQINKLEELWKDNPGASLEDLEKPGVDEEPQQVLLRYEDAYQYQNVFGPLVKHEADYDKKLKESQTQDNITIRWDLGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDLGVPWKGIGHVIKVPDNYGDEIAIELRSGANAPVDITHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVTIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLSRQGNGPVLVCAPSNIAVDQLTEKIDKTGLKVVRLCAKSREAIESPVSFLALHNQISNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRIKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTRLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQQVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRAKYGVIIVGNPKALSKQPLWNNLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLLNTINPGGRFMSTAMYDAREALIPGSAYDRSSAPGRPSNMYFQTHDQIGMIGAGPGHMAMNLPIPLNLVMPPMPPPSYMGHANGPAGMKGGMKGKPGRGRGQRSRGGGAQGNGPHSQASQDGASQPFSQGPLTQGYMNMSQPSQMSQPGLSQPELSQDSYLGDEFKSQMDVALSQDSTYQGERAYQHGVTGLSQY

Target ORF information:

RefSeq Version	XM_020936155.1
Organism	Boleophthalmus pectinirostris(great blue-spotted mudskipper)
Definition	Boleophthalmus pectinirostris regulator of nonsense transcripts 1-like (LOC110170500), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020936155.1

ORF Insert Sequence:

ATGAGTGTGG AGGCGTACGG GCCGAGCTCG CAGACGCTCA CCTTCCTGGA CACGGAGGAA 
GCGGAGCTGA TCGGAGCGGA CACGCAGGGC TCCGAGTACG ACTTCACCGA CTTCACCCTG 
CCGAGCCAGA GCCAGGGCCC GGGCCACACG CAGGGCCACA CGCAGGGCCA CGCGCAGGGC 
CAGCTGGACG CGCAGTTGAA CGGTCCAGAT GACGGGCTTC ACAACGGAGG AGTGGACGAC 
ACTGTGGCCA AAACCACTCA GCTGCTGCAG GAGCTCAACT TCGAGGAGGA CGAGGAGGAC 
GCGTACTACA CCAAAGACCT GCCCGTGCAC GCCTGCAGTT ACTGCGGCAT CCACGACCCG 
GCCTGCGTCG TTTACTGCAA CACCAGCAAG AAATGGTTCT GTAACGGCCG AGGAAACACG 
TCCGGGAGCC ACATCGTGAA CCACCTGGTG CGTGCGAAGT GTAAAGAGGT GACTCTGCAC 
AAAGACGGGC CCCTGGGCGA GACGGTGCTC GAGTGCTACA ACTGCGGCTG TAGAAACGTC 
TTCCTGCTCG GCTTCATCCC CGCCAAGGCC GACTCTGTGG TGGTGCTCCT CTGCAGACAG 
CCCTGCGCCA GTCAGAGCTC CCTGAAGGAC ATCAACTGGG ACAGCTCCCA GTGGCAGCCG 
CTCATCCAGG ACCGCTGCTT CCTGTCGTGG CTCGTCAAGA TCCCGTCGGA GCAGGAGCAG 
CTCAGGGCCC GGCAGATCTC GGCGCAGCAG ATCAACAAGC TGGAGGAACT GTGGAAGGAT 
AACCCCGGGG CGTCGCTGGA GGACCTGGAG AAGCCCGGAG TGGACGAAGA GCCTCAGCAG 
GTTCTGCTCA GATACGAAGA CGCGTATCAG TATCAGAACG TGTTCGGACC TCTGGTCAAA 
CACGAGGCCG ACTACGACAA GAAGCTCAAA GAGTCTCAGA CCCAAGACAA CATCACCATC 
CGCTGGGACC TGGGTCTGAA CAAAAAGCGC ATAGCGTACT TTACCCTGCC CAAGACGGAC 
TCAGACATGA GGCTGATGCA GGGAGACGAG ATCTGCCTGA GGTACAAAGG AGACCTGGGC 
GTCCCGTGGA AGGGCATCGG GCACGTCATC AAAGTCCCCG ACAATTATGG AGATGAAATC 
GCCATCGAGC TTCGCAGCGG AGCCAACGCT CCCGTCGACA TCACCCACAA CTTCCAGGTG 
GACTTTGTCT GGAAGTCCAC GTCTTTCGAC AGGATGCAGA GCGCCCTGAA GACGTTCGCC 
GTGGACGAGA CGTCCGTGTC CGGGTACATT TACCACAAAC TCCTGGGGCA CGAGGTGGAG 
GACGTCACCA TCAAGTGTCA GCTCCCCAAA CGCTTCACCG CCCAGGGCCT GCCCGATCTC 
AACCACTCAC AGGTGTACGC TGTGAAGACG GTGCTGCAGA GGCCTCTGAG TCTGATCCAG 
GGACCTCCAG GAACCGGCAA AACTGTCACG TCTGCCACCA TCGTGTACCA CCTGTCGCGC 
CAGGGCAACG GGCCGGTGCT GGTGTGTGCT CCCAGTAACA TCGCCGTGGA TCAGCTCACA 
GAAAAGATCG ACAAAACTGG ACTGAAGGTG GTGCGTCTGT GCGCCAAGAG CAGAGAGGCC 
ATCGAGTCCC CGGTGTCTTT CCTCGCCCTG CACAACCAGA TCAGCAACAT GGACAGCATG 
CCCGAGCTGC AGAAGCTCCA GCAGCTGAAG GACGAGACCG GAGAGCTGTC CTCCGCTGAC 
GAGAAGAGAT ACAGAGCTCT GAAGAGGACC GCGGAGAGGG AGCTGCTCAT GAATGCAGAC 
GTGATCTGCT GCACCTGCGT CGGAGCCGGA GATCCTCGTT TGGCCAAGAT GCAGTTTCGC 
TCGATTCTGA TCGACGAGAG CACTCAGGCC ACAGAGCCCG AGTGCATGGT CCCCGTGGTG 
CTCGGGGCCA AACAGCTGAT CCTGGTGGGC GATCACTGCC AGCTGGGTCC TGTGGTGATG 
TGTAAGAAGG CGGCCAAAGC GGGTCTGTCC CAGTCTCTGT TCGAGCGTCT GGTGGTCCTG 
GGCATCAGAC CCATCCGGCT CCAGGTGCAG TACCGCATGC ACCCCGCCCT CAGCGCCTTC 
CCCTCCAACA TCTTCTACGA GGGCTCCCTG CAGAACGGCG TCACGGCAGC CGATCGCATA 
AAGAAAGGCT TTGACTTCCA GTGGCCGCAG CCGGACAAAC CCATGTTCTT CTACGTGACT 
CAGGGACAGG AGGAGATCGC CAGCTCCGGG ACCTCCTACC TCAACAGGAC TGAAGCGGCC 
AACGTGGAGA AGATCACCAC TCGTCTGCTG AAAGCTGGAG CCAAACCGGA TCAGATCGGC 
ATCATCACCC CGTACGAGGG ACAGCGCTCC TACCTGGTCC AGTACATGCA GTTCAGCGGC 
TCCCTGCACA CCAAACTCTA CCAGCAGGTG GAGATAGCGA GCGTGGACGC GTTCCAGGGC 
CGAGAGAAGG ACTTCATCAT CCTGTCGTGC GTCCGAGCCA ACGAGCACCA GGGCATCGGC 
TTCCTCAACG ATCCGCGCCG CCTCAACGTC GCCCTCACCA GAGCCAAGTA CGGCGTGATC 
ATCGTGGGGA ACCCCAAGGC CCTGTCGAAG CAGCCGCTGT GGAACAACCT CCTGAACTAC 
TACAAAGAGC AGAAGGTCCT GGTGGAGGGG CCCCTGAACA ACCTGAGGGA GAGTCTCATG 
CAGTTCAGCA AACCCCGAAA ACTGCTCAAC ACCATCAACC CCGGAGGGCG CTTCATGAGC 
ACAGCCATGT ACGACGCCAG AGAGGCTCTG ATCCCCGGCT CCGCCTACGA CCGCTCCAGC 
GCACCGGGAC GTCCCTCTAA CATGTACTTC CAGACCCATG ATCAGATCGG GATGATCGGG 
GCAGGACCGG GGCACATGGC CATGAACCTG CCCATCCCCC TGAACCTGGT GATGCCCCCG 
ATGCCCCCGC CCAGTTACAT GGGCCACGCC AACGGCCCCG CAGGGATGAA AGGCGGGATG 
AAGGGAAAGC CGGGCCGCGG TCGGGGTCAG AGGTCACGTG GAGGCGGGGC TCAGGGGAAC 
GGCCCCCACA GCCAGGCCAG TCAGGACGGG GCCTCCCAGC CCTTCTCCCA GGGGCCCCTC 
ACTCAGGGCT ACATGAACAT GAGCCAACCC TCTCAGATGA GCCAACCGGG GCTGTCCCAA 
CCCGAGCTCT CGCAGGACAG TTACCTCGGA GACGAGTTCA AGTCCCAGAT GGACGTGGCC 
CTGTCCCAGG ACTCCACCTA CCAGGGCGAG AGAGCCTATC AGCACGGGGT GACCGGACTG 
TCCCAGTACT AG