BDBG_05413 cDNA ORF clone, Blastomyces gilchristii SLH14081

Gene
Clones
gRNAs

Summary

Gene Symbol	BDBG_05413
Entrez Gene ID	8503989
Full Name	NAD-specific glutamate dehydrogenase
Gene Type	protein-coding
Organism	Blastomyces gilchristii SLH14081

mRNA and Protein(s)

mRNA	Protein	Name
XM_031722134.1	XP_031578950.1	NAD-specific glutamate dehydrogenase
XM_002623859.1	XP_002623905.1	NAD-specific glutamate dehydrogenase

Pathways from BioSystems
Gene Ontology

The following BDBG_05413 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BDBG_05413 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAl114012	XM_031722134.1 Latest version!	Blastomyces gilchristii SLH14081 NAD-specific glutamate dehydrogenase (BDBG_05413), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAl03016	XM_002623859.1 Latest version!	Ajellomyces dermatitidis SLH14081 NAD-specific glutamate dehydrogenase, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BDBG_05413 ( XM_031722134.1 ) cDNA ORF clone, Blastomyces gilchristii SLH14081 -> XP_031578950.1 Blastomyces gilchristii SLH14081 NAD-specific glutamate dehydrogenase (BDBG_05413), partial mRNA.

Price & Availability↑

CloneID	OAl114012
Clone ID Related Accession (Same CDS sequence)	XM_031722134.1
Accession Version	XM_031722134.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Blastomyces gilchristii SLH14081
Product	NAD-specific glutamate dehydrogenase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101672). COMPLETENESS: incomplete on both ends.

ATGCAGACCG CCTTATACGA TGAGTCCATC ATTCAGATCT CAGCGACAAA TCCTCTCTCC 
TACCAGGAGT ACTCTGGCGC GGGCACCGTC ACCGCCGGCT TCCAGAGACA TTCCGTGGTT 
CACATGCAGT ATGATAAGGT CATCCGCACT CCGGGCGGTC AACCGTCGCC CCAGCCTACG 
CATCTGGGTA TTCCAGGCGG CACGCACCGC GTGTTGTCGG AGGAAGGGCC GGGGTATGTT 
GCGGCGAAGT TTGAGGGGAA GAAGAAGCAG ATGGAGGCTG CTATGACATT ATGGACCAGC 
TCGAGGAGAA GGGATTCATC CCGATCGAAT TCGTCTCGTC GGAAACAACT GGTTCTACAA 
CATGCTTGGC ATCGACGACA TGTACTTCAA ACCGAGACGT CGACGCCATC GTCAGCCATA 
TCCTCTCCCT CTACGCCGCC AAGGTGGCCG CATATGCCCG TGATGATTAA GCGTCTGGAG 
ATCAGGTTGG ATAAGGAGGC TGCCGACCAC GCTGTCTACA TCGACACTAG CAAGCCTGGA 
ATAAGCGCCA TCGACGGGCC GCGGTATGAA CAGCGCATCG ACGAGAAATA CATCAATGGC 
TCCACCCCGA AGAACGCCTA TCGTGTGGAA ACTTTCCGTT CCGCCAGCTC CCTTCCAGAT 
AACCATGAGC AGCAGCTGCG TTGCTACTTC GTCTACAAGT GTCACTTCAG CGAGCCGCAT 
CCGGACCCGG AGGAGACGAA TATTGAAATT GTGGGTGAGA AGCGCTTCTT GCAAAAGGCT 
ACCGCCAACA CAAAGGCCAT CTACCAGGAG ATCATTATCA ATGCTGTGGC TAGATCTGGC 
CCTGTCATTG AGATGTTTGA GATTGAAGGC AGCCGTGAAA AGAGACTGGT GATCGCGTAC 
CGCCAGGGTT CCGCTATGGG CATGTTCAGC GCCCTGTCTG ACCTCTACCA CTACTACCGC 
CTTACAAGCT CGCGGAAGTA CCTAGAGAAC TTCTCCAATG GTATCACTGT AGTCTCTCTC 
TACCTGAAAC CCATGGAAGG CGCAGAAATC TCCGGGAAAT ACCCACCCAT CGAAGCTGCG 
ATCCATCAGA TCATGAAGGA GATCTCCTTG CTGTACTGCA TTCCTCAAAA CAAGTTCCAG 
AGCCATTTCG CCAGTGGCCG TCTTAGCTTA CAAGAAACTA TCTACGCCCA CTGCGTATGG 
GTGTTCGTGC AGCAGTTCCT GAACCGCCTG GGATCCGAAT ACACCTCCCT AACCGCCCTC 
CTCGATTCGA GCAATAGCTC ACACGCTGAG CTCCTTTCAA AGATCAAGAA GAGACTTCGT 
ACCGAAACCT TCACCTCAGA CTATATCCTT GAGATCATCA ACAAATACCC CCAACTCATC 
CACAAGCTGT ACCTTGATTT CGCCACAACG CATTACGTTC AGATGGTTGA GGGAGTGCCC 
GATGACTTCC TCCCCACGCT CAGCTACTTG CGTCTGCAGG TAGATGAGCC CCTGGACCAC 
GACCGCCTCG ACGAGCTGAT CTCCAAAACC GTGGTCAGCG AGAATGACGA GATGGTCATG 
AACTCGTTCC GTGTCTTCAA CGCTGCGGTT CTCAAGACTA ACTTCTACAC CCCAACCAAA 
GTCGCTCTCA GTTTCCGTCT CAACGCGGAT TTCCTGCCCG AACACGAATA CCCCCAGCGC 
CTGTACGGAA TGTTCCTTAT CATTAGCTCT GAGTTCCGCG GCTTCCATCT CCGTTTCCGC 
GATATCTCCC GTGGCGGTAT CCGGATAGTC AAGAGTCGTG ACAAGGAAGC CTATGCCATC 
AATGCCCGCT CGCTATTTGA TGAAAATTAC AACCTGGCCA ATACCCAGCA GCGGAAGAAC 
AAGGATATCC CCGAAGGCGG CGCCAAGGGT GTTATCCTGC TCGACGTCAA CCATCAGGAC 
AAGGCGAGAG TGGCCTTTGA GAAATACATC GACAGTATCC TCGACCTGCT CCTCCCGCCC 
GCCAGCCCTG GTATCAAAGA CCCCATCGTC GACCGCCACG GACAGGATGA GATCCTCTTC 
ATGGGTCCTG ATGAGAACAC TGCTGAGCTG GTCGACTGGG CAACTCACCA TGCGCGAAAG 
CGGGGTGCCC CCTGGTGGAA GTCCTTCTTT ACAGGCAAGA GCCCCAAACT CGGCGGTATC 
CCCCATGACA GATATGGTAT GACCACCTTA TCCGTCCGCC AGTACGTGCT CGGCATCTAC 
CGCAAGACAG GCATCGACCC ATCCACAGTC CGCAAAATGC AAACCGGCGG ACCCGATGGC 
GACCTCGGCT CCAACGAAAT CCTCCTCGGG AACGAGAAGT ACTGCGCCAT CGTCGATGGG 
TCTGGCGTCC TGGTCGACCC GCAGGGCCTC GACCGCGACG AGCTCGTCAG ACTAGCCAAG 
AAGCGCGCCA TGATTGTCCA CTATGATGCA TCCAAGCTAT CCCCTGAGGG CTACCGTGTT 
CTCGTCGACG AGACCAACGT AACCCTCCCG GACGGCGAAG TCGTCCACAA CGGCACCGTC 
TTCCGCAACA CCTTCCACCT CCGCGGTGGG CGGGCCTTTG ACATCTTCGT CCCCTGCGGC 
GGGCGCCCGG AGTCCATTGA CCTCAGCAAC GTCAGCAAAC TGATCGAGAA CGGCAAAGCC 
GTTATCCCCT ACATTGTCGA GGGCGCCAAC CTCTTCCTAA CCCAGGATAG CAAGATCCGC 
CTGGAAAAAG CCGGCTGCGT CCTCTTCAAA GACGCCTCCG TCAACAAGGG TGGCGTCACC 
TCTTCATCCC TCGAGGTCCT CGCCTCCCTA TCCTTCGACG ACAAGGGCTT CGAGGATCAC 
ATGTGCGTGC GCGAGGACGG GACCATCCCG CCATTCTACG ACGCGTACGT GCGCGAGGTC 
CAGGAGACCA TCCAGCGCAA CGCGCGCTTG GAGTTCGAGG CCATCTGGCG CGAGCATGAG 
GAGACTGGGA TGCCGCGCAG TATGATCAGT GATAAGCTTA GTCTTGCGAT TACGAAGCTG 
GATGAGGAGT TGCAGGAGAC GGAGCTGTGG GATAATACGG TTTTGAGAGA GGATGTGCTG 
CGGGATGCGT TGCCGAAGCT TTTGTTGGAG AAGATTGGAC TGGAGACGAT TTTGGAGAGG 
GTCCCATCCA ACTACCTCCG CTCCATCTTC GGCAGCTACC TTGCCAGCCG CTTCGTCTAC 
GAGTACGGCA GCAGCCCGAG CCAGTTTTCT TTCTTCGATT TCATGTCCAA GAGAACCGCG 
AAGCTCATAG ATCGACAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031578950.1
CDS	1..3264
Translation	MQTALYDESIIQISATNPLSYQEYSGAGTVTAGFQRHSVVHMQYDKVIRTPGGQPSPQPTHLGIPGGTHRVLSEEGPGYVAAKFEGKKKQMEAAMTLWTSSRRRDSSRSNSSRRKQLVLQHAWHRRHVLQTETSTPSSAISSPSTPPRWPHMPVMIKRLEIRLDKEAADHAVYIDTSKPGISAIDGPRYEQRIDEKYINGSTPKNAYRVETFRSASSLPDNHEQQLRCYFVYKCHFSEPHPDPEETNIEIVGEKRFLQKATANTKAIYQEIIINAVARSGPVIEMFEIEGSREKRLVIAYRQGSAMGMFSALSDLYHYYRLTSSRKYLENFSNGITVVSLYLKPMEGAEISGKYPPIEAAIHQIMKEISLLYCIPQNKFQSHFASGRLSLQETIYAHCVWVFVQQFLNRLGSEYTSLTALLDSSNSSHAELLSKIKKRLRTETFTSDYILEIINKYPQLIHKLYLDFATTHYVQMVEGVPDDFLPTLSYLRLQVDEPLDHDRLDELISKTVVSENDEMVMNSFRVFNAAVLKTNFYTPTKVALSFRLNADFLPEHEYPQRLYGMFLIISSEFRGFHLRFRDISRGGIRIVKSRDKEAYAINARSLFDENYNLANTQQRKNKDIPEGGAKGVILLDVNHQDKARVAFEKYIDSILDLLLPPASPGIKDPIVDRHGQDEILFMGPDENTAELVDWATHHARKRGAPWWKSFFTGKSPKLGGIPHDRYGMTTLSVRQYVLGIYRKTGIDPSTVRKMQTGGPDGDLGSNEILLGNEKYCAIVDGSGVLVDPQGLDRDELVRLAKKRAMIVHYDASKLSPEGYRVLVDETNVTLPDGEVVHNGTVFRNTFHLRGGRAFDIFVPCGGRPESIDLSNVSKLIENGKAVIPYIVEGANLFLTQDSKIRLEKAGCVLFKDASVNKGGVTSSSLEVLASLSFDDKGFEDHMCVREDGTIPPFYDAYVREVQETIQRNARLEFEAIWREHEETGMPRSMISDKLSLAITKLDEELQETELWDNTVLREDVLRDALPKLLLEKIGLETILERVPSNYLRSIFGSYLASRFVYEYGSSPSQFSFFDFMSKRTAKLIDRQK

Target ORF information:

RefSeq Version	XM_031722134.1
Organism	Blastomyces gilchristii SLH14081
Definition	Blastomyces gilchristii SLH14081 NAD-specific glutamate dehydrogenase (BDBG_05413), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031722134.1

ORF Insert Sequence:

ATGCAGACCG CCTTATACGA TGAGTCCATC ATTCAGATCT CAGCGACAAA TCCTCTCTCC 
TACCAGGAGT ACTCTGGCGC GGGCACCGTC ACCGCCGGCT TCCAGAGACA TTCCGTGGTT 
CACATGCAGT ATGATAAGGT CATCCGCACT CCGGGCGGTC AACCGTCGCC CCAGCCTACG 
CATCTGGGTA TTCCAGGCGG CACGCACCGC GTGTTGTCGG AGGAAGGGCC GGGGTATGTT 
GCGGCGAAGT TTGAGGGGAA GAAGAAGCAG ATGGAGGCTG CTATGACATT ATGGACCAGC 
TCGAGGAGAA GGGATTCATC CCGATCGAAT TCGTCTCGTC GGAAACAACT GGTTCTACAA 
CATGCTTGGC ATCGACGACA TGTACTTCAA ACCGAGACGT CGACGCCATC GTCAGCCATA 
TCCTCTCCCT CTACGCCGCC AAGGTGGCCG CATATGCCCG TGATGATTAA GCGTCTGGAG 
ATCAGGTTGG ATAAGGAGGC TGCCGACCAC GCTGTCTACA TCGACACTAG CAAGCCTGGA 
ATAAGCGCCA TCGACGGGCC GCGGTATGAA CAGCGCATCG ACGAGAAATA CATCAATGGC 
TCCACCCCGA AGAACGCCTA TCGTGTGGAA ACTTTCCGTT CCGCCAGCTC CCTTCCAGAT 
AACCATGAGC AGCAGCTGCG TTGCTACTTC GTCTACAAGT GTCACTTCAG CGAGCCGCAT 
CCGGACCCGG AGGAGACGAA TATTGAAATT GTGGGTGAGA AGCGCTTCTT GCAAAAGGCT 
ACCGCCAACA CAAAGGCCAT CTACCAGGAG ATCATTATCA ATGCTGTGGC TAGATCTGGC 
CCTGTCATTG AGATGTTTGA GATTGAAGGC AGCCGTGAAA AGAGACTGGT GATCGCGTAC 
CGCCAGGGTT CCGCTATGGG CATGTTCAGC GCCCTGTCTG ACCTCTACCA CTACTACCGC 
CTTACAAGCT CGCGGAAGTA CCTAGAGAAC TTCTCCAATG GTATCACTGT AGTCTCTCTC 
TACCTGAAAC CCATGGAAGG CGCAGAAATC TCCGGGAAAT ACCCACCCAT CGAAGCTGCG 
ATCCATCAGA TCATGAAGGA GATCTCCTTG CTGTACTGCA TTCCTCAAAA CAAGTTCCAG 
AGCCATTTCG CCAGTGGCCG TCTTAGCTTA CAAGAAACTA TCTACGCCCA CTGCGTATGG 
GTGTTCGTGC AGCAGTTCCT GAACCGCCTG GGATCCGAAT ACACCTCCCT AACCGCCCTC 
CTCGATTCGA GCAATAGCTC ACACGCTGAG CTCCTTTCAA AGATCAAGAA GAGACTTCGT 
ACCGAAACCT TCACCTCAGA CTATATCCTT GAGATCATCA ACAAATACCC CCAACTCATC 
CACAAGCTGT ACCTTGATTT CGCCACAACG CATTACGTTC AGATGGTTGA GGGAGTGCCC 
GATGACTTCC TCCCCACGCT CAGCTACTTG CGTCTGCAGG TAGATGAGCC CCTGGACCAC 
GACCGCCTCG ACGAGCTGAT CTCCAAAACC GTGGTCAGCG AGAATGACGA GATGGTCATG 
AACTCGTTCC GTGTCTTCAA CGCTGCGGTT CTCAAGACTA ACTTCTACAC CCCAACCAAA 
GTCGCTCTCA GTTTCCGTCT CAACGCGGAT TTCCTGCCCG AACACGAATA CCCCCAGCGC 
CTGTACGGAA TGTTCCTTAT CATTAGCTCT GAGTTCCGCG GCTTCCATCT CCGTTTCCGC 
GATATCTCCC GTGGCGGTAT CCGGATAGTC AAGAGTCGTG ACAAGGAAGC CTATGCCATC 
AATGCCCGCT CGCTATTTGA TGAAAATTAC AACCTGGCCA ATACCCAGCA GCGGAAGAAC 
AAGGATATCC CCGAAGGCGG CGCCAAGGGT GTTATCCTGC TCGACGTCAA CCATCAGGAC 
AAGGCGAGAG TGGCCTTTGA GAAATACATC GACAGTATCC TCGACCTGCT CCTCCCGCCC 
GCCAGCCCTG GTATCAAAGA CCCCATCGTC GACCGCCACG GACAGGATGA GATCCTCTTC 
ATGGGTCCTG ATGAGAACAC TGCTGAGCTG GTCGACTGGG CAACTCACCA TGCGCGAAAG 
CGGGGTGCCC CCTGGTGGAA GTCCTTCTTT ACAGGCAAGA GCCCCAAACT CGGCGGTATC 
CCCCATGACA GATATGGTAT GACCACCTTA TCCGTCCGCC AGTACGTGCT CGGCATCTAC 
CGCAAGACAG GCATCGACCC ATCCACAGTC CGCAAAATGC AAACCGGCGG ACCCGATGGC 
GACCTCGGCT CCAACGAAAT CCTCCTCGGG AACGAGAAGT ACTGCGCCAT CGTCGATGGG 
TCTGGCGTCC TGGTCGACCC GCAGGGCCTC GACCGCGACG AGCTCGTCAG ACTAGCCAAG 
AAGCGCGCCA TGATTGTCCA CTATGATGCA TCCAAGCTAT CCCCTGAGGG CTACCGTGTT 
CTCGTCGACG AGACCAACGT AACCCTCCCG GACGGCGAAG TCGTCCACAA CGGCACCGTC 
TTCCGCAACA CCTTCCACCT CCGCGGTGGG CGGGCCTTTG ACATCTTCGT CCCCTGCGGC 
GGGCGCCCGG AGTCCATTGA CCTCAGCAAC GTCAGCAAAC TGATCGAGAA CGGCAAAGCC 
GTTATCCCCT ACATTGTCGA GGGCGCCAAC CTCTTCCTAA CCCAGGATAG CAAGATCCGC 
CTGGAAAAAG CCGGCTGCGT CCTCTTCAAA GACGCCTCCG TCAACAAGGG TGGCGTCACC 
TCTTCATCCC TCGAGGTCCT CGCCTCCCTA TCCTTCGACG ACAAGGGCTT CGAGGATCAC 
ATGTGCGTGC GCGAGGACGG GACCATCCCG CCATTCTACG ACGCGTACGT GCGCGAGGTC 
CAGGAGACCA TCCAGCGCAA CGCGCGCTTG GAGTTCGAGG CCATCTGGCG CGAGCATGAG 
GAGACTGGGA TGCCGCGCAG TATGATCAGT GATAAGCTTA GTCTTGCGAT TACGAAGCTG 
GATGAGGAGT TGCAGGAGAC GGAGCTGTGG GATAATACGG TTTTGAGAGA GGATGTGCTG 
CGGGATGCGT TGCCGAAGCT TTTGTTGGAG AAGATTGGAC TGGAGACGAT TTTGGAGAGG 
GTCCCATCCA ACTACCTCCG CTCCATCTTC GGCAGCTACC TTGCCAGCCG CTTCGTCTAC 
GAGTACGGCA GCAGCCCGAG CCAGTTTTCT TTCTTCGATT TCATGTCCAA GAGAACCGCG 
AAGCTCATAG ATCGACAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BDBG_05413 ( XM_002623859.1 ) cDNA ORF clone, Blastomyces gilchristii SLH14081 -> XP_002623905.1 Ajellomyces dermatitidis SLH14081 NAD-specific glutamate dehydrogenase, mRNA.

Price & Availability↑

CloneID	OAl03016
Clone ID Related Accession (Same CDS sequence)	XM_002623859.1
Accession Version	XM_002623859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2009-10-08
Organism	Blastomyces gilchristii SLH14081
Product	NAD-specific glutamate dehydrogenase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101672). COMPLETENESS: incomplete on both ends.

ATGCAGACCG CCTTATACGA TGAGTCCATC ATTCAGATCT CAGCGACAAA TCCTCTCTCC 
TACCAGGAGT ACTCTGGCGC GGGCACCGTC ACCGCCGGCT TCCAGAGACA TTCCGTGGTT 
CACATGCAGT ATGATAAGGT CATCCGCACT CCGGGCGGTC AACCGTCGCC CCAGCCTACG 
CATCTGGGTA TTCCAGGCGG CACGCACCGC GTGTTGTCGG AGGAAGGGCC GGGGTATGTT 
GCGGCGAAGT TTGAGGGGAA GAAGAAGCAG ATGGAGGCTG TTATGGACCA GCTCGAGGAG 
AAGGGATTCA TCCCGATCGA ATTCGTCTCG TCGGAAACAA CTGGTTCTAC AACATGCTTG 
GCATCGACGA CATGTACTTC AAACCGAGAC GTCGACGCCA TCGTCAGCCA TATCCTCTCC 
CTCTACGCCG CCAAGGAGGC TGCCGACCAC GCTGTCTACA TCGACACTAG CAAGCCTGGA 
ATAAGCGCCA TCGACGGGCC GCGGTATGAA CAGCGCATCG ACGAGAAATA CATCAATGGC 
TCCACCCCGA AGAACGCCTA TCGTGTGGAA ACTTTCCGTT CCGCCAGCTC CCTTCCAGAT 
AACCATGAGC AGCAGCTGCG TTGCTACTTC GTCTACAAGT GTCACTTCAG CGAGCCGCAT 
CCGGACCCGG AGGAGACGAA TATTGAAATT GTGGGTGAGA AGCGCTTCTT GCAAAAGGCT 
ACCGCCAACA CAAAGGCCAT CTACCAGGAG ATCATTATCA ATGCTGTGGC TAGATCTGGC 
CCTGTCATTG AGATGTTTGA GATTGAAGGC AGCCGTGAAA AGAGACTGGT GATCGCGTAC 
CGCCAGGGTT CCGCTATGGG CATGTTCAGC GCCCTGTCTG ACCTCTACCA CTACTACCGC 
CTTACAAGCT CGCGGAAGTA CCTAGAGAAC TTCTCCAATG GTATCACTGT AGTCTCTCTC 
TACCTGAAAC CCATGGAAGG CGCAGAAATC TCCGGGAAAT ACCCACCCAT CGAAGCTGCG 
ATCCATCAGA TCATGAAGGA GATCTCCTTG CTGTACTGCA TTCCTCAAAA CAAGTTCCAG 
AGCCATTTCG CCAGTGGCCG TCTTAGCTTA CAAGAAACTA TCTACGCCCA CTGCGTATGG 
GTGTTCGTGC AGCAGTTCCT GAACCGCCTG GGATCCGAAT ACACCTCCCT AACCGCCCTC 
CTCGATTCGA GCAATAGCTC ACACGCTGAG CTCCTTTCAA AGATCAAGAA GAGACTTCGT 
ACCGAAACCT TCACCTCAGA CTATATCCTT GAGATCATCA ACAAATACCC CCAACTCATC 
CACAAGCTGT ACCTTGATTT CGCCACAACG CATTACGTTC AGATGGTTGA GGGAGTGCCC 
GATGACTTCC TCCCCACGCT CAGCTACTTG CGTCTGCAGG TAGATGAGCC CCTGGACCAC 
GACCGCCTCG ACGAGCTGAT CTCCAAAACC GTGGTCAGCG AGAATGACGA GATGGTCATG 
AACTCGTTCC GTGTCTTCAA CGCTGCGGTT CTCAAGACTA ACTTCTACAC CCCAACCAAA 
GTCGCTCTCA GTTTCCGTCT CAACGCGGAT TTCCTGCCCG AACACGAATA CCCCCAGCGC 
CTGTACGGAA TGTTCCTTAT CATTAGCTCT GAGTTCCGCG GCTTCCATCT CCGTTTCCGC 
GATATCTCCC GTGGCGGTAT CCGGATAGTC AAGAGTCGTG ACAAGGAAGC CTATGCCATC 
AATGCCCGCT CGCTATTTGA TGAAAATTAC AACCTGGCCA ATACCCAGCA GCGGAAGAAC 
AAGGATATCC CCGAAGGCGG CGCCAAGGGT GTTATCCTGC TCGACGTCAA CCATCAGGAC 
AAGGCGAGAG TGGCCTTTGA GAAATACATC GACAGTATCC TCGACCTGCT CCTCCCGCCC 
GCCAGCCCTG GTATCAAAGA CCCCATCGTC GACCGCCACG GACAGGATGA GATCCTCTTC 
ATGGGTCCTG ATGAGAACAC TGCTGAGCTG GTCGACTGGG CAACTCACCA TGCGCGAAAG 
CGGGGTGCCC CCTGGTGGAA GTCCTTCTTT ACAGGCAAGA GCCCCAAACT CGGCGGTATC 
CCCCATGACA GATATGGTAT GACCACCTTA TCCGTCCGCC AGTACGTGCT CGGCATCTAC 
CGCAAGACAG GCATCGACCC ATCCACAGTC CGCAAAATGC AAACCGGCGG ACCCGATGGC 
GACCTCGGCT CCAACGAAAT CCTCCTCGGG AACGAGAAGT ACTGCGCCAT CGTCGATGGG 
TCTGGCGTCC TGGTCGACCC GCAGGGCCTC GACCGCGACG AGCTCGTCAG ACTAGCCAAG 
AAGCGCGCCA TGATTGTCCA CTATGATGCA TCCAAGCTAT CCCCTGAGGG CTACCGTGTT 
CTCGTCGACG AGACCAACGT AACCCTCCCG GACGGCGAAG TCGTCCACAA CGGCACCGTC 
TTCCGCAACA CCTTCCACCT CCGCGGTGGG CGGGCCTTTG ACATCTTCGT CCCCTGCGGC 
GGGCGCCCGG AGTCCATTGA CCTCAGCAAC GTCAGCAAAC TGATCGAGAA CGGCAAAGCC 
GTTATCCCCT ACATTGTCGA GGGCGCCAAC CTCTTCCTAA CCCAGGATAG CAAGATCCGC 
CTGGAAAAAG CCGGCTGCGT CCTCTTCAAA GACGCCTCCG TCAACAAGGG TGGCGTCACC 
TCTTCATCCC TCGAGGTCCT CGCCTCCCTA TCCTTCGACG ACAAGGGCTT CGAGGATCAC 
ATGTGCGTGC GCGAGGACGG GACCATCCCG CCATTCTACG ACGCGTACGT GCGCGAGGTC 
CAGGAGACCA TCCAGCGCAA CGCGCGCTTG GAGTTCGAGG CCATCTGGCG CGAGCATGAG 
GAGACTGGGA TGCCGCGCAG TATGATCAGT GATAAGCTTA GTCTTGCGAT TACGAAGCTG 
GATGAGGAGT TGCAGGAGAC GGAGCTGTGG GATAATACGG TTTTGAGAGA GGATGTGCTG 
CGGGATGCGT TGCCGAAGCT TTTGTTGGAG AAGATTGGAC TGGAGACGAT TTTGGAGAGG 
GTCCCATCCA ACTACCTCCG CTCCATCTTC GGCAGCTACC TTGCCAGCCG CTTCGTCTAC 
GAGTACGGCA GCAGCCCGAG CCAGTTTTCT TTCTTCGATT TCATGTCCAA GAGAACCGCG 
AAGCTCATAG ATCGACAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002623905.1
CDS	1..3204
Translation	MQTALYDESIIQISATNPLSYQEYSGAGTVTAGFQRHSVVHMQYDKVIRTPGGQPSPQPTHLGIPGGTHRVLSEEGPGYVAAKFEGKKKQMEAVMDQLEEKGFIPIEFVSSETTGSTTCLASTTCTSNRDVDAIVSHILSLYAAKEAADHAVYIDTSKPGISAIDGPRYEQRIDEKYINGSTPKNAYRVETFRSASSLPDNHEQQLRCYFVYKCHFSEPHPDPEETNIEIVGEKRFLQKATANTKAIYQEIIINAVARSGPVIEMFEIEGSREKRLVIAYRQGSAMGMFSALSDLYHYYRLTSSRKYLENFSNGITVVSLYLKPMEGAEISGKYPPIEAAIHQIMKEISLLYCIPQNKFQSHFASGRLSLQETIYAHCVWVFVQQFLNRLGSEYTSLTALLDSSNSSHAELLSKIKKRLRTETFTSDYILEIINKYPQLIHKLYLDFATTHYVQMVEGVPDDFLPTLSYLRLQVDEPLDHDRLDELISKTVVSENDEMVMNSFRVFNAAVLKTNFYTPTKVALSFRLNADFLPEHEYPQRLYGMFLIISSEFRGFHLRFRDISRGGIRIVKSRDKEAYAINARSLFDENYNLANTQQRKNKDIPEGGAKGVILLDVNHQDKARVAFEKYIDSILDLLLPPASPGIKDPIVDRHGQDEILFMGPDENTAELVDWATHHARKRGAPWWKSFFTGKSPKLGGIPHDRYGMTTLSVRQYVLGIYRKTGIDPSTVRKMQTGGPDGDLGSNEILLGNEKYCAIVDGSGVLVDPQGLDRDELVRLAKKRAMIVHYDASKLSPEGYRVLVDETNVTLPDGEVVHNGTVFRNTFHLRGGRAFDIFVPCGGRPESIDLSNVSKLIENGKAVIPYIVEGANLFLTQDSKIRLEKAGCVLFKDASVNKGGVTSSSLEVLASLSFDDKGFEDHMCVREDGTIPPFYDAYVREVQETIQRNARLEFEAIWREHEETGMPRSMISDKLSLAITKLDEELQETELWDNTVLREDVLRDALPKLLLEKIGLETILERVPSNYLRSIFGSYLASRFVYEYGSSPSQFSFFDFMSKRTAKLIDRQK

Target ORF information:

RefSeq Version	XM_002623859.1
Organism	Blastomyces gilchristii SLH14081
Definition	Ajellomyces dermatitidis SLH14081 NAD-specific glutamate dehydrogenase, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002623859.1

ORF Insert Sequence:

ATGCAGACCG CCTTATACGA TGAGTCCATC ATTCAGATCT CAGCGACAAA TCCTCTCTCC 
TACCAGGAGT ACTCTGGCGC GGGCACCGTC ACCGCCGGCT TCCAGAGACA TTCCGTGGTT 
CACATGCAGT ATGATAAGGT CATCCGCACT CCGGGCGGTC AACCGTCGCC CCAGCCTACG 
CATCTGGGTA TTCCAGGCGG CACGCACCGC GTGTTGTCGG AGGAAGGGCC GGGGTATGTT 
GCGGCGAAGT TTGAGGGGAA GAAGAAGCAG ATGGAGGCTG TTATGGACCA GCTCGAGGAG 
AAGGGATTCA TCCCGATCGA ATTCGTCTCG TCGGAAACAA CTGGTTCTAC AACATGCTTG 
GCATCGACGA CATGTACTTC AAACCGAGAC GTCGACGCCA TCGTCAGCCA TATCCTCTCC 
CTCTACGCCG CCAAGGAGGC TGCCGACCAC GCTGTCTACA TCGACACTAG CAAGCCTGGA 
ATAAGCGCCA TCGACGGGCC GCGGTATGAA CAGCGCATCG ACGAGAAATA CATCAATGGC 
TCCACCCCGA AGAACGCCTA TCGTGTGGAA ACTTTCCGTT CCGCCAGCTC CCTTCCAGAT 
AACCATGAGC AGCAGCTGCG TTGCTACTTC GTCTACAAGT GTCACTTCAG CGAGCCGCAT 
CCGGACCCGG AGGAGACGAA TATTGAAATT GTGGGTGAGA AGCGCTTCTT GCAAAAGGCT 
ACCGCCAACA CAAAGGCCAT CTACCAGGAG ATCATTATCA ATGCTGTGGC TAGATCTGGC 
CCTGTCATTG AGATGTTTGA GATTGAAGGC AGCCGTGAAA AGAGACTGGT GATCGCGTAC 
CGCCAGGGTT CCGCTATGGG CATGTTCAGC GCCCTGTCTG ACCTCTACCA CTACTACCGC 
CTTACAAGCT CGCGGAAGTA CCTAGAGAAC TTCTCCAATG GTATCACTGT AGTCTCTCTC 
TACCTGAAAC CCATGGAAGG CGCAGAAATC TCCGGGAAAT ACCCACCCAT CGAAGCTGCG 
ATCCATCAGA TCATGAAGGA GATCTCCTTG CTGTACTGCA TTCCTCAAAA CAAGTTCCAG 
AGCCATTTCG CCAGTGGCCG TCTTAGCTTA CAAGAAACTA TCTACGCCCA CTGCGTATGG 
GTGTTCGTGC AGCAGTTCCT GAACCGCCTG GGATCCGAAT ACACCTCCCT AACCGCCCTC 
CTCGATTCGA GCAATAGCTC ACACGCTGAG CTCCTTTCAA AGATCAAGAA GAGACTTCGT 
ACCGAAACCT TCACCTCAGA CTATATCCTT GAGATCATCA ACAAATACCC CCAACTCATC 
CACAAGCTGT ACCTTGATTT CGCCACAACG CATTACGTTC AGATGGTTGA GGGAGTGCCC 
GATGACTTCC TCCCCACGCT CAGCTACTTG CGTCTGCAGG TAGATGAGCC CCTGGACCAC 
GACCGCCTCG ACGAGCTGAT CTCCAAAACC GTGGTCAGCG AGAATGACGA GATGGTCATG 
AACTCGTTCC GTGTCTTCAA CGCTGCGGTT CTCAAGACTA ACTTCTACAC CCCAACCAAA 
GTCGCTCTCA GTTTCCGTCT CAACGCGGAT TTCCTGCCCG AACACGAATA CCCCCAGCGC 
CTGTACGGAA TGTTCCTTAT CATTAGCTCT GAGTTCCGCG GCTTCCATCT CCGTTTCCGC 
GATATCTCCC GTGGCGGTAT CCGGATAGTC AAGAGTCGTG ACAAGGAAGC CTATGCCATC 
AATGCCCGCT CGCTATTTGA TGAAAATTAC AACCTGGCCA ATACCCAGCA GCGGAAGAAC 
AAGGATATCC CCGAAGGCGG CGCCAAGGGT GTTATCCTGC TCGACGTCAA CCATCAGGAC 
AAGGCGAGAG TGGCCTTTGA GAAATACATC GACAGTATCC TCGACCTGCT CCTCCCGCCC 
GCCAGCCCTG GTATCAAAGA CCCCATCGTC GACCGCCACG GACAGGATGA GATCCTCTTC 
ATGGGTCCTG ATGAGAACAC TGCTGAGCTG GTCGACTGGG CAACTCACCA TGCGCGAAAG 
CGGGGTGCCC CCTGGTGGAA GTCCTTCTTT ACAGGCAAGA GCCCCAAACT CGGCGGTATC 
CCCCATGACA GATATGGTAT GACCACCTTA TCCGTCCGCC AGTACGTGCT CGGCATCTAC 
CGCAAGACAG GCATCGACCC ATCCACAGTC CGCAAAATGC AAACCGGCGG ACCCGATGGC 
GACCTCGGCT CCAACGAAAT CCTCCTCGGG AACGAGAAGT ACTGCGCCAT CGTCGATGGG 
TCTGGCGTCC TGGTCGACCC GCAGGGCCTC GACCGCGACG AGCTCGTCAG ACTAGCCAAG 
AAGCGCGCCA TGATTGTCCA CTATGATGCA TCCAAGCTAT CCCCTGAGGG CTACCGTGTT 
CTCGTCGACG AGACCAACGT AACCCTCCCG GACGGCGAAG TCGTCCACAA CGGCACCGTC 
TTCCGCAACA CCTTCCACCT CCGCGGTGGG CGGGCCTTTG ACATCTTCGT CCCCTGCGGC 
GGGCGCCCGG AGTCCATTGA CCTCAGCAAC GTCAGCAAAC TGATCGAGAA CGGCAAAGCC 
GTTATCCCCT ACATTGTCGA GGGCGCCAAC CTCTTCCTAA CCCAGGATAG CAAGATCCGC 
CTGGAAAAAG CCGGCTGCGT CCTCTTCAAA GACGCCTCCG TCAACAAGGG TGGCGTCACC 
TCTTCATCCC TCGAGGTCCT CGCCTCCCTA TCCTTCGACG ACAAGGGCTT CGAGGATCAC 
ATGTGCGTGC GCGAGGACGG GACCATCCCG CCATTCTACG ACGCGTACGT GCGCGAGGTC 
CAGGAGACCA TCCAGCGCAA CGCGCGCTTG GAGTTCGAGG CCATCTGGCG CGAGCATGAG 
GAGACTGGGA TGCCGCGCAG TATGATCAGT GATAAGCTTA GTCTTGCGAT TACGAAGCTG 
GATGAGGAGT TGCAGGAGAC GGAGCTGTGG GATAATACGG TTTTGAGAGA GGATGTGCTG 
CGGGATGCGT TGCCGAAGCT TTTGTTGGAG AAGATTGGAC TGGAGACGAT TTTGGAGAGG 
GTCCCATCCA ACTACCTCCG CTCCATCTTC GGCAGCTACC TTGCCAGCCG CTTCGTCTAC 
GAGTACGGCA GCAGCCCGAG CCAGTTTTCT TTCTTCGATT TCATGTCCAA GAGAACCGCG 
AAGCTCATAG ATCGACAGAA ATGA