RPA1 cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	RPA1
Entrez Gene ID	105003892
Full Name	replication protein A1
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010861327.1	XP_010859629.1	replication protein A 70 kDa DNA-binding subunit

Pathways from BioSystems
Gene Ontology

The following RPA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi80510	XM_010861327.1 Latest version!	Bison bison bison replication protein A1, 70kDa (RPA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPA1 ( XM_010861327.1 ) cDNA ORF clone, Bison bison bison -> XP_010859629.1 Bison bison bison replication protein A1, 70kDa (RPA1), mRNA.

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CloneID	OBi80510
Clone ID Related Accession (Same CDS sequence)	XM_010861327.1
Accession Version	XM_010861327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	replication protein A 70 kDa DNA-binding subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011495033.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGCC ACCTGAGCGA GGGGGCCATT GCGGCCATCA TGCAACAGGG AGATACATCT 
ATAAAACCCA TCCTCCAAGT CATTAACATC CGTCCCATTA CTACAGGGAA TAGTCCACCC 
CGTTATCGAC TGCTTATGAG TGATGGATTG AATACTCTAT CTTCATTCAT GTTGGCAACA 
CAGCTGAACC CTCTTGTTGA GGAAGAGCGA TTGTCTAGCA ACTGTATTTG CCAGATTAAC 
CGATTTATTG TGAACACGCT GAAAGATGGA AGGAGAGTGG TTATTTTGAT GGAGTTAGAA 
GTTTTGAAAT CAGCTGAAGC AGTTGGATCA AAGATTGGCA ACCCAGTGCC TTATAATGAA 
GGACACGGAC AGCAGCCAGT AGTTCCTCCT CCTGTCTCAG CAGCCAGTCC ACCAACCAGC 
AGGCCCCAGC AACAGAATGG GAGCCCAGGA ATGGCTTCCA CTGCATCTAA GGCTTTTGGT 
GCCTCAAAGA CATTTGGAAA AGCTGGTGGT ACCAGCCAAG TGAACAGCTC TGGGGGAACA 
CAGGCCAAAG TGGTACCCAT TGCCAGCCTC ACCCCGTACC AATCCAAGTG GACTATCTGT 
GCTCGTGTCA CCAACAAAAG TCAGATCCGT ACCTGGAGCA ACTCCCGAGG GGAAGGGAAG 
CTTTTCTCCA TAGAACTGGT TGATGAGAGT GGTGAAATCA GAGCGACTGC TTTCAATGAG 
CAAGCGGACA AGTTTTTCCC CCTTATTGAC GTGAACAAGG TCTATTATTT CTCTAAAGGC 
ACCCTGAAGA TCGCCAACAA ACAGTTCACA GCCGTCAAAA ACGACTACGA GATGACCTTC 
AACAACGAGA CTTCTGTCAT GCCCTGTGAA GACGGTCATC ACTTACCCAC AGTTCAGTTT 
GATTTCACAG GGATTGGCGA CCTGGAGAGC AAATCTAAGG ACTCGCTTGT AGACATAATC 
GGGATCTGCA AGAACTACGA AGATGTCACA AAAATCATAG TGAAGTCTAA CAACAGAGAA 
GTCTCGAAGA GAAATATCTA TCTGATGGAT ATGTCAGGGA AAGTGGTGAA CGCTACCCTG 
TGGGGAGACG ATGCTGATAA ATTTGATGGC TCTAGACAAC CCGTGATGGC CATCAAAGGA 
GCCAGAGTTT CTGATTTTGG TGGACGGAGC CTCTCTGTCC TATCTTCAAG CACCATCATT 
GTGAACCCTG ACATCCCAGA GGCCTATAAG CTTCGTGGAT GGTTTGACTC AGAAGGACAA 
GCCTTAGATG GCATTTCCAT CTCTGATCTA AAGAGTGGAG GAGCAGGTGG GAGCAATACC 
AACTGGAAAA CATTATATGA GGTCAAGTCG GAGAACCTGG GCCAAGGTGA CAAGCCTGAC 
TATTTTAGCT CTGTGGCAAC TGTGGTGTAT CTTCGCAAAG AGAACTGTAT GTACCAGGCC 
TGCCCAACTC AGGATTGTAA CAAGAAAGTG ATCGACCAGC AGAACGGATT GTACCGCTGC 
GAGAAGTGTG ACAGTGAATT CCCCAATTTC AAGTACCGCA TGATCCTGTC AGTCAATATT 
GCCGATTTTC AAGAGAATCA GTGGGTCACA TGTTTCCAGG AGTCTGCAGA GGCCATCCTT 
GGACAAAGCA CTGCTTATCT TGGGGAATTA AAAGAGAAGA ACGAGCAAGC ATTTGAGGAA 
GTTTTCCAGA ATGCCAACTT TCGATCTTTT ACGTTCAGGA TCAGGGTCAA ACTGGAGACC 
TACAATGATG AGTCTCGAAT TAAGGCCACG GTGGTGGACG TGAAGCCTGT GGACTACAGA 
GAATACGGCA GAAGGCTGGT CATGAACATC AGGAGGAACG CGGCGATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010859629.1
CDS	39..1889
Translation	MVGHLSEGAIAAIMQQGDTSIKPILQVINIRPITTGNSPPRYRLLMSDGLNTLSSFMLATQLNPLVEEERLSSNCICQINRFIVNTLKDGRRVVILMELEVLKSAEAVGSKIGNPVPYNEGHGQQPVVPPPVSAASPPTSRPQQQNGSPGMASTASKAFGASKTFGKAGGTSQVNSSGGTQAKVVPIASLTPYQSKWTICARVTNKSQIRTWSNSRGEGKLFSIELVDESGEIRATAFNEQADKFFPLIDVNKVYYFSKGTLKIANKQFTAVKNDYEMTFNNETSVMPCEDGHHLPTVQFDFTGIGDLESKSKDSLVDIIGICKNYEDVTKIIVKSNNREVSKRNIYLMDMSGKVVNATLWGDDADKFDGSRQPVMAIKGARVSDFGGRSLSVLSSSTIIVNPDIPEAYKLRGWFDSEGQALDGISISDLKSGGAGGSNTNWKTLYEVKSENLGQGDKPDYFSSVATVVYLRKENCMYQACPTQDCNKKVIDQQNGLYRCEKCDSEFPNFKYRMILSVNIADFQENQWVTCFQESAEAILGQSTAYLGELKEKNEQAFEEVFQNANFRSFTFRIRVKLETYNDESRIKATVVDVKPVDYREYGRRLVMNIRRNAAM

Target ORF information:

RefSeq Version	XM_010861327.1
Organism	Bison bison bison
Definition	Bison bison bison replication protein A1, 70kDa (RPA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010861327.1

ORF Insert Sequence:

ATGGTGGGCC ACCTGAGCGA GGGGGCCATT GCGGCCATCA TGCAACAGGG AGATACATCT 
ATAAAACCCA TCCTCCAAGT CATTAACATC CGTCCCATTA CTACAGGGAA TAGTCCACCC 
CGTTATCGAC TGCTTATGAG TGATGGATTG AATACTCTAT CTTCATTCAT GTTGGCAACA 
CAGCTGAACC CTCTTGTTGA GGAAGAGCGA TTGTCTAGCA ACTGTATTTG CCAGATTAAC 
CGATTTATTG TGAACACGCT GAAAGATGGA AGGAGAGTGG TTATTTTGAT GGAGTTAGAA 
GTTTTGAAAT CAGCTGAAGC AGTTGGATCA AAGATTGGCA ACCCAGTGCC TTATAATGAA 
GGACACGGAC AGCAGCCAGT AGTTCCTCCT CCTGTCTCAG CAGCCAGTCC ACCAACCAGC 
AGGCCCCAGC AACAGAATGG GAGCCCAGGA ATGGCTTCCA CTGCATCTAA GGCTTTTGGT 
GCCTCAAAGA CATTTGGAAA AGCTGGTGGT ACCAGCCAAG TGAACAGCTC TGGGGGAACA 
CAGGCCAAAG TGGTACCCAT TGCCAGCCTC ACCCCGTACC AATCCAAGTG GACTATCTGT 
GCTCGTGTCA CCAACAAAAG TCAGATCCGT ACCTGGAGCA ACTCCCGAGG GGAAGGGAAG 
CTTTTCTCCA TAGAACTGGT TGATGAGAGT GGTGAAATCA GAGCGACTGC TTTCAATGAG 
CAAGCGGACA AGTTTTTCCC CCTTATTGAC GTGAACAAGG TCTATTATTT CTCTAAAGGC 
ACCCTGAAGA TCGCCAACAA ACAGTTCACA GCCGTCAAAA ACGACTACGA GATGACCTTC 
AACAACGAGA CTTCTGTCAT GCCCTGTGAA GACGGTCATC ACTTACCCAC AGTTCAGTTT 
GATTTCACAG GGATTGGCGA CCTGGAGAGC AAATCTAAGG ACTCGCTTGT AGACATAATC 
GGGATCTGCA AGAACTACGA AGATGTCACA AAAATCATAG TGAAGTCTAA CAACAGAGAA 
GTCTCGAAGA GAAATATCTA TCTGATGGAT ATGTCAGGGA AAGTGGTGAA CGCTACCCTG 
TGGGGAGACG ATGCTGATAA ATTTGATGGC TCTAGACAAC CCGTGATGGC CATCAAAGGA 
GCCAGAGTTT CTGATTTTGG TGGACGGAGC CTCTCTGTCC TATCTTCAAG CACCATCATT 
GTGAACCCTG ACATCCCAGA GGCCTATAAG CTTCGTGGAT GGTTTGACTC AGAAGGACAA 
GCCTTAGATG GCATTTCCAT CTCTGATCTA AAGAGTGGAG GAGCAGGTGG GAGCAATACC 
AACTGGAAAA CATTATATGA GGTCAAGTCG GAGAACCTGG GCCAAGGTGA CAAGCCTGAC 
TATTTTAGCT CTGTGGCAAC TGTGGTGTAT CTTCGCAAAG AGAACTGTAT GTACCAGGCC 
TGCCCAACTC AGGATTGTAA CAAGAAAGTG ATCGACCAGC AGAACGGATT GTACCGCTGC 
GAGAAGTGTG ACAGTGAATT CCCCAATTTC AAGTACCGCA TGATCCTGTC AGTCAATATT 
GCCGATTTTC AAGAGAATCA GTGGGTCACA TGTTTCCAGG AGTCTGCAGA GGCCATCCTT 
GGACAAAGCA CTGCTTATCT TGGGGAATTA AAAGAGAAGA ACGAGCAAGC ATTTGAGGAA 
GTTTTCCAGA ATGCCAACTT TCGATCTTTT ACGTTCAGGA TCAGGGTCAA ACTGGAGACC 
TACAATGATG AGTCTCGAAT TAAGGCCACG GTGGTGGACG TGAAGCCTGT GGACTACAGA 
GAATACGGCA GAAGGCTGGT CATGAACATC AGGAGGAACG CGGCGATGTG A