GFI1 cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	GFI1
Entrez Gene ID	105003372
Full Name	growth factor independent 1 transcriptional repressor
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010860508.1	XP_010858810.1	zinc finger protein Gfi-1

Pathways from BioSystems
Gene Ontology

The following GFI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GFI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi79940	XM_010860508.1 Latest version!	Bison bison bison growth factor independent 1 transcription repressor (GFI1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GFI1 ( XM_010860508.1 ) cDNA ORF clone, Bison bison bison -> XP_010858810.1 Bison bison bison growth factor independent 1 transcription repressor (GFI1), mRNA.

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CloneID	OBi79940
Clone ID Related Accession (Same CDS sequence)	XM_010860508.1
Accession Version	XM_010860508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	zinc finger protein Gfi-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011495027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 33% of CDS bases ##RefSeq-Attributes-END##

ATGCCGCGTT CGTTCCTGGT TAAGAGCAAA AAAGCTCACA GTTACCACCA GCCGCGCTCC 
CCGGGACCTG ACTATTCCCT CCGCCTGGAG AATGTACTGG CGCCAGGCGG AGCAGACGGC 
ATCTCGAGCA CAGGCGGGGC GAAGGCGGAG CTCAGGGGCC GTGTGTCCCC CGAGTCTCAG 
CTGACCGAAG CCCCCGACAG AGCCTCCGCG TCCCCTGGCA GCTGTGAGGG CAGCGTGTGC 
GATCGGAGCT CGGAGTTTGA AGACTTTTGG AGGCCTCCGT CGCCTTCTGT GTCTCCAGGT 
AGGGACCTGT TGGGGGCTCT GGGGCCCGGG GAGGGAGGCG GGGACAGGGA CCCCGCGCTG 
AGCAATGAAC CCGCGCGGCT TTGGGCACAG AAGTGCCAAA TCGATTGCGG AGAATCTTTT 
CGAGAAGAGG GATTTGTTCT TGCTTCCCGA GGGGAGGCAT TGGCTTCTCT AGGCCAGCGT 
CAGCCTGCTC GCGGGGGTCG GCTGCTTGGA TCCCAGGCGA CGGGACCTGG GCTGTTCATC 
CCTAACATTC ATTGGCACGG AGCTAGTGGG CGAAAGAAAA TAAATGAGTC GCCACCGGAG 
AGTCCTTCTT GGCTGTGCTG GACACCGCCC CGCAAGGACT GGGCGCCGCC GGAATCTCCG 
ACGGAAACTC GAAGTGAGAA GCCACTTCTC ACTGAAGTGA CTAGCCTGAG CTGGATCTGT 
TCACAGAACT GCAGTGTTTG TGAACAGATC TGTAAATCCA TCCCAGGAAA GAAAAAGGAC 
GGAGTGGGAA CACGTAGGGG CCGGGAAAGC GCCGGCACTG GGCCCTCTGC CCGCAGGCTC 
GTCCAGGTCC GAAACAGTGG CCGCTTTCTC GTCCGGCACA GCTCGGCTCT GCTCCAAACG 
TTTGTAAAGC ATGGCGCGCC CTTCTCTACG CGGCCGCCAC ATGTGCCGGT CCAGTCTAAA 
AAGACCCTCT TAACGTCGTG CGCTTCGGCT GTCCTGGCAC AGAAAGTCCT CGCCCGATAT 
CATTTTAAGT TTCTGTGTGC TGCCTTGCCG CCTCCAGTGG TTCTCGGAGG CCCCAGGGAC 
CCGGCCAGGC CGGCGTCCGC GCCCCTCGGC CCCTCTCAGC GGCCCTCTCC CCGGCCCCAC 
CCGCCTCAGG TGTTCTCCAC ACCGCACGGG CTCGAGGTGC ACGTGCGCAG GTCCCACAGC 
GGCACAAGAC CCTTTGCGTG CGAGATTTGC GGCAAGACCT TCGGGCACGC GGTGAGCCTG 
GAGCAGCACA AAGCCGTGCA CTCGCAGGAA CGGAGCTTTG ACTGTAAGAT CTGTGGCAAG 
AGCTTCAAGA GGTCATCTAC GCTGTCCACA CATCTGCTCA TCCACTCAGA CACCCGGCCC 
TACCCCTGTC AGTACTGCGG CAAGAGGTTC CATCAGAAGT CAGACATGAA GAAACACACC 
TTCATCCACA CTGGCGAGAA GCCCCACAAG TGCCAGGTGT GTGGCAAGGC ATTCAGCCAG 
AGCTCCAACC TCATCACCCA CAGCCGCAAG CACACAGGCT TCAAGCCCTT CGGCTGCGAC 
CTGTGTGGAA AAGGCTTCCA GAGGAAGGTG GACCTCAGGC GGCACCGGGA GACGCAGCAT 
GGGCTCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010858810.1
CDS	1648..3279
Translation	MPRSFLVKSKKAHSYHQPRSPGPDYSLRLENVLAPGGADGISSTGGAKAELRGRVSPESQLTEAPDRASASPGSCEGSVCDRSSEFEDFWRPPSPSVSPGRDLLGALGPGEGGGDRDPALSNEPARLWAQKCQIDCGESFREEGFVLASRGEALASLGQRQPARGGRLLGSQATGPGLFIPNIHWHGASGRKKINESPPESPSWLCWTPPRKDWAPPESPTETRSEKPLLTEVTSLSWICSQNCSVCEQICKSIPGKKKDGVGTRRGRESAGTGPSARRLVQVRNSGRFLVRHSSALLQTFVKHGAPFSTRPPHVPVQSKKTLLTSCASAVLAQKVLARYHFKFLCAALPPPVVLGGPRDPARPASAPLGPSQRPSPRPHPPQVFSTPHGLEVHVRRSHSGTRPFACEICGKTFGHAVSLEQHKAVHSQERSFDCKICGKSFKRSSTLSTHLLIHSDTRPYPCQYCGKRFHQKSDMKKHTFIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFGCDLCGKGFQRKVDLRRHRETQHGLK

Target ORF information:

RefSeq Version	XM_010860508.1
Organism	Bison bison bison
Definition	Bison bison bison growth factor independent 1 transcription repressor (GFI1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010860508.1

ORF Insert Sequence:

ATGCCGCGTT CGTTCCTGGT TAAGAGCAAA AAAGCTCACA GTTACCACCA GCCGCGCTCC 
CCGGGACCTG ACTATTCCCT CCGCCTGGAG AATGTACTGG CGCCAGGCGG AGCAGACGGC 
ATCTCGAGCA CAGGCGGGGC GAAGGCGGAG CTCAGGGGCC GTGTGTCCCC CGAGTCTCAG 
CTGACCGAAG CCCCCGACAG AGCCTCCGCG TCCCCTGGCA GCTGTGAGGG CAGCGTGTGC 
GATCGGAGCT CGGAGTTTGA AGACTTTTGG AGGCCTCCGT CGCCTTCTGT GTCTCCAGGT 
AGGGACCTGT TGGGGGCTCT GGGGCCCGGG GAGGGAGGCG GGGACAGGGA CCCCGCGCTG 
AGCAATGAAC CCGCGCGGCT TTGGGCACAG AAGTGCCAAA TCGATTGCGG AGAATCTTTT 
CGAGAAGAGG GATTTGTTCT TGCTTCCCGA GGGGAGGCAT TGGCTTCTCT AGGCCAGCGT 
CAGCCTGCTC GCGGGGGTCG GCTGCTTGGA TCCCAGGCGA CGGGACCTGG GCTGTTCATC 
CCTAACATTC ATTGGCACGG AGCTAGTGGG CGAAAGAAAA TAAATGAGTC GCCACCGGAG 
AGTCCTTCTT GGCTGTGCTG GACACCGCCC CGCAAGGACT GGGCGCCGCC GGAATCTCCG 
ACGGAAACTC GAAGTGAGAA GCCACTTCTC ACTGAAGTGA CTAGCCTGAG CTGGATCTGT 
TCACAGAACT GCAGTGTTTG TGAACAGATC TGTAAATCCA TCCCAGGAAA GAAAAAGGAC 
GGAGTGGGAA CACGTAGGGG CCGGGAAAGC GCCGGCACTG GGCCCTCTGC CCGCAGGCTC 
GTCCAGGTCC GAAACAGTGG CCGCTTTCTC GTCCGGCACA GCTCGGCTCT GCTCCAAACG 
TTTGTAAAGC ATGGCGCGCC CTTCTCTACG CGGCCGCCAC ATGTGCCGGT CCAGTCTAAA 
AAGACCCTCT TAACGTCGTG CGCTTCGGCT GTCCTGGCAC AGAAAGTCCT CGCCCGATAT 
CATTTTAAGT TTCTGTGTGC TGCCTTGCCG CCTCCAGTGG TTCTCGGAGG CCCCAGGGAC 
CCGGCCAGGC CGGCGTCCGC GCCCCTCGGC CCCTCTCAGC GGCCCTCTCC CCGGCCCCAC 
CCGCCTCAGG TGTTCTCCAC ACCGCACGGG CTCGAGGTGC ACGTGCGCAG GTCCCACAGC 
GGCACAAGAC CCTTTGCGTG CGAGATTTGC GGCAAGACCT TCGGGCACGC GGTGAGCCTG 
GAGCAGCACA AAGCCGTGCA CTCGCAGGAA CGGAGCTTTG ACTGTAAGAT CTGTGGCAAG 
AGCTTCAAGA GGTCATCTAC GCTGTCCACA CATCTGCTCA TCCACTCAGA CACCCGGCCC 
TACCCCTGTC AGTACTGCGG CAAGAGGTTC CATCAGAAGT CAGACATGAA GAAACACACC 
TTCATCCACA CTGGCGAGAA GCCCCACAAG TGCCAGGTGT GTGGCAAGGC ATTCAGCCAG 
AGCTCCAACC TCATCACCCA CAGCCGCAAG CACACAGGCT TCAAGCCCTT CGGCTGCGAC 
CTGTGTGGAA AAGGCTTCCA GAGGAAGGTG GACCTCAGGC GGCACCGGGA GACGCAGCAT 
GGGCTCAAGT GA