ITCH cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	ITCH
Entrez Gene ID	104999328
Full Name	itchy E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010854874.1	XP_010853176.1	E3 ubiquitin-protein ligase Itchy homolog

Pathways from BioSystems
Gene Ontology

The following ITCH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITCH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi75752	XM_010854874.1 Latest version!	Bison bison bison itchy E3 ubiquitin protein ligase (ITCH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ITCH ( XM_010854874.1 ) cDNA ORF clone, Bison bison bison -> XP_010853176.1 Bison bison bison itchy E3 ubiquitin protein ligase (ITCH), mRNA.

Price & Availability↑

CloneID	OBi75752
Clone ID Related Accession (Same CDS sequence)	XM_010854874.1
Accession Version	XM_010854874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	E3 ubiquitin-protein ligase Itchy homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494924.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA GTGGATCACA GCTTGGTTCA ATGGGTAGTT TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGGCCAAGT 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA GCCTCTTACA GTTATTGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGGATTG 
GATATCTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA ACCAGCAGAG ACAATAGGAG ATTTGTCAGT CTGTCTTGAT 
GGGCTGCAAC TAGAGTCTGA AGTTGTTGCC AATGGTGAAA CTGCATGTTC AGAAAGTGCT 
TCACACAATG ATGATGGCTC CAGGCCCAAG GATGAAACAA GAGCGAACGC AAGTGGATCA 
GATGACCCTG AGGATGCAGG GTCTGGTGAA AATAGGAGGA TCAATGGGAA TAATTCTCCA 
TCACTCTCAA ATGGCGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCAC GTAGACCAGC ATCTGTCAAT GGCTCACCAT CTACCACTTC TGAAAGTGAT 
GGCTCTAGTA CAGGCTCTCT GCCACCAACA AATACAAATT CAAGTACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGTG CAGGCCCTAG ACCATTAAAT 
CCTGTACCTC AAGCTCCCCT CCCACCTGGT TGGGAGCAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCAGA ACCTCTACCT 
CCTGGCTGGG AGCGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCAGTCC GGAACTATGA ACAATGGCAG 
CTCCAGCGTA GTCAACTTCA GGGAGCTATG CAGCAGTTTA ACCAGAGGTT CATTTATGGG 
AATCAAGATT TGTTTGCTAC ATCACAAAAT AAAGAATTTG ATCCCCTGGG TCCCCTGCCG 
CCTGGTTGGG AGAAGAGAAC GGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAAT CAAGGTCAAT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACGGTAGAC GGAATTCCGT ATTTCGTGGA CCACAATAGA 
AGAACAACCA CCTATATAGA TCCCCGCACC GGAAAATCTG CCTTAGACAA TGGACCCCAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAG GTTCAATATT TCCGTTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC 
TTTCAGCAGA TAATGAGCTT CAGTCCTCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA 
CATGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGGAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATCTT GAATAAACCA GTTGGACTGA AGGATTTAGA ATCGATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA GTGTGATTTG 
GAAATGTATT TCTCCATTGA TAAAGAAATC TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCCAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AAGAATATAT CAGAATAGTA 
GCTGAATGGA GATTGTCTCG TGGTGTTGAA GAACAGACGC AAGCTTTCTT TGAGGGCTTT 
AATGAAATTC TTCCCCAGCA GTATCTACAG TACTTCGATG CAAAGGAATT AGAGGTTCTT 
TTATGTGGGA TGCAAGAGAT TGATTTGAAT GATTGGCAAA GACATGCCAT CTACCGTCAC 
TATACCAGGA CAAGTAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATCGATAAT 
GAGAAGAGAA TGCGACTTTT GCAGTTTGTT ACTGGGACCT GCCGACTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGGCCA CAGAAGTTCT GCATTGAAAA AGTCGGGAAA 
GAAAATTGGT TGCCTAGAAG TCATACCTGT TTTAACCGCC TGGACCTGCC ACCCTACAAG 
AGCTATGAGC AACTGAAGGA AAAGCTGTTA TTTGCTATTG AAGAAACAGA AGGCTTTGGA 
CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010853176.1
CDS	262..2850
Translation	MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAGLDIYETLKSNNMKLEEVVVTLQLVGDKEPAETIGDLSVCLDGLQLESEVVANGETACSESASHNDDGSRPKDETRANASGSDDPEDAGSGENRRINGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTTSESDGSSTGSLPPTNTNSSTSEGATSGLIIPLTISGGAGPRPLNPVPQAPLPPGWEQRVDQHGRVYYVDHIEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRNQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSIDKEILGEIKSHDLKPNGGNILVTEENKEEYIRIVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_010854874.1
Organism	Bison bison bison
Definition	Bison bison bison itchy E3 ubiquitin protein ligase (ITCH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010854874.1

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA GCTTGGTTCA ATGGGTAGTT TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGGCCAAGT 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA GCCTCTTACA GTTATTGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGGATTG 
GATATCTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA ACCAGCAGAG ACAATAGGAG ATTTGTCAGT CTGTCTTGAT 
GGGCTGCAAC TAGAGTCTGA AGTTGTTGCC AATGGTGAAA CTGCATGTTC AGAAAGTGCT 
TCACACAATG ATGATGGCTC CAGGCCCAAG GATGAAACAA GAGCGAACGC AAGTGGATCA 
GATGACCCTG AGGATGCAGG GTCTGGTGAA AATAGGAGGA TCAATGGGAA TAATTCTCCA 
TCACTCTCAA ATGGCGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCAC GTAGACCAGC ATCTGTCAAT GGCTCACCAT CTACCACTTC TGAAAGTGAT 
GGCTCTAGTA CAGGCTCTCT GCCACCAACA AATACAAATT CAAGTACATC TGAAGGAGCA 
ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGTG CAGGCCCTAG ACCATTAAAT 
CCTGTACCTC AAGCTCCCCT CCCACCTGGT TGGGAGCAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCAGA ACCTCTACCT 
CCTGGCTGGG AGCGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCAGTCC GGAACTATGA ACAATGGCAG 
CTCCAGCGTA GTCAACTTCA GGGAGCTATG CAGCAGTTTA ACCAGAGGTT CATTTATGGG 
AATCAAGATT TGTTTGCTAC ATCACAAAAT AAAGAATTTG ATCCCCTGGG TCCCCTGCCG 
CCTGGTTGGG AGAAGAGAAC GGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAAT CAAGGTCAAT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACGGTAGAC GGAATTCCGT ATTTCGTGGA CCACAATAGA 
AGAACAACCA CCTATATAGA TCCCCGCACC GGAAAATCTG CCTTAGACAA TGGACCCCAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAG GTTCAATATT TCCGTTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC 
TTTCAGCAGA TAATGAGCTT CAGTCCTCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA 
CATGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGGAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATCTT GAATAAACCA GTTGGACTGA AGGATTTAGA ATCGATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA GTGTGATTTG 
GAAATGTATT TCTCCATTGA TAAAGAAATC TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCCAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AAGAATATAT CAGAATAGTA 
GCTGAATGGA GATTGTCTCG TGGTGTTGAA GAACAGACGC AAGCTTTCTT TGAGGGCTTT 
AATGAAATTC TTCCCCAGCA GTATCTACAG TACTTCGATG CAAAGGAATT AGAGGTTCTT 
TTATGTGGGA TGCAAGAGAT TGATTTGAAT GATTGGCAAA GACATGCCAT CTACCGTCAC 
TATACCAGGA CAAGTAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATCGATAAT 
GAGAAGAGAA TGCGACTTTT GCAGTTTGTT ACTGGGACCT GCCGACTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGGCCA CAGAAGTTCT GCATTGAAAA AGTCGGGAAA 
GAAAATTGGT TGCCTAGAAG TCATACCTGT TTTAACCGCC TGGACCTGCC ACCCTACAAG 
AGCTATGAGC AACTGAAGGA AAAGCTGTTA TTTGCTATTG AAGAAACAGA AGGCTTTGGA 
CAAGAGTGA