ISM2 cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	ISM2
Entrez Gene ID	104991808
Full Name	isthmin 2
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010844414.1	XP_010842716.1	LOW QUALITY PROTEIN: isthmin-2

Pathways from BioSystems
Gene Ontology

The following ISM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ISM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi68105	XM_010844414.1 Latest version!	Bison bison bison isthmin 2 (ISM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ISM2 ( XM_010844414.1 ) cDNA ORF clone, Bison bison bison -> XP_010842716.1 Bison bison bison isthmin 2 (ISM2), mRNA.

Price & Availability↑

CloneID	OBi68105
Clone ID Related Accession (Same CDS sequence)	XM_010844414.1
Accession Version	XM_010844414.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	LOW QUALITY PROTEIN: isthmin-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494773.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 40% of CDS bases frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGGCGAAAC ACGGCGCTTA TGCCCACCAG GCTGGGCCAT CTAAATCACC TGGAAAGGCT 
GCAGACCCTG AACTCCTGTT CCCCCAAGTC TCCCTTCTCT GTGCTCAGAG GCTGCAGACA 
GGCCGACTAC ACAAAGTCAC AGGGACAGAC TTTCCCAGGT CCACATGGGC CTTGACCTCA 
GCCTCCCCAG ATCCCAGCTC TCCCAGAGAA GAGGAGGAGA CCCCTCTGCT CTCCAGGGCC 
CACCTCCAGG CAGGGTCACG TCGACACAGA TGTCGGGCGC TCCCTGAATC AGCACCCCTG 
GTCCTAGATA AGACGCCCTC ACCTGGGACC CTGAAGGACG CTCCCTTGCT GCTGGAGCTG 
CAGAAATTGC CAGGATTGAC CAATACAGAT TTGAGTGCCT TGAACCCCAA CATCCAGATG 
AGGGCTACAA GGGCGGCCAT GACCCAGCGG ATTCCTGGAG AAAAGCTGGG CTCAACAGCT 
CAGCTCCGAG GTGAAGTGGA TCCCCAGGAT GAGCTGGAGG TGGACCTGAT GGCTGAGCCC 
AACAATCGCT GGTCCGAGGG CGCCCCCTGC TGGCTGCCTG CTGAGGAACT CTTCTGGCCT 
CTCTCTTGGG GCTACTCAGA GGGTGAGGAA GAGAGGCCCC GTCTCAAGGG CAGAGCCTCA 
GGAGAAGAGC AGGAGGAGGA AGAGGAGGAT GACCCCCCAG AGTACAGTGA GGGTGAGGAC 
CAGGAGGACA GTGAGGAACA CGGTAGAGAT GATCAGGAGC CGGGCTTCAG TGGGGCCGCA 
GGAGGCTGGG AGCAGGGCCC ACTGTCTCCC AGAGACTGGG CCTTTGAGGA GCCTGATCGC 
TATGACTATG AGCTTCAGGA GGAGTGGAGC CCCTGGTCTC TCTGCAGTGG GACCTGTGGC 
AGTGGCCAGC AGAGGACTCA GCCCTGCAGC TACACCTGCA TTGCCTCCAA GTCCTGCGCC 
TGCGACCTGC CCCCGTCCTG GTCGTTCTGC CCCCAGCTCG GGTGGGCTGA CACGTCCCTT 
GTTCCCCTGC CACCTCCCCT GCCCTCTGCA AATGTGGACA GCTGTGAAAA GTGGCTGAAC 
TGCAAGAGTG ACTTCCTGGC CAAGTACCTG AGCCAGGTGC TGCGGGCCCT GCCCAGCTGC 
CCATGCGCAT ACGCGTTGGA GGCCGTGTCC AGCGCCGTGA GCCTGTGGGA GGAGCATCAA 
GGCCGCAGCT TCCGCTGGAG GGATGCCGGC GGNNNNNNNN NNNNNGTGCC GGTCCACAGC 
CCCTTCTGCC TGCGCTCTCT GNNCAGCACG CCGGCAGCCC AGCACTGCTG CTACGACGTG 
AGCAGCCGGC TGCTGACCCG GGGCAAGGGT GCCGGCGCGC CCGACTTGGT CAGCATCGAC 
GTCTCGCCCG AGCTTCACTT CAAGGTGGAC ACGCTGCCCT GGATCCCGTG TAAGGCGGAC 
TGCACCCACT ACCACGCGGT GCGGCCCGCC AACAATGGCC GAGCCTGCGC GGACAACCCT 
CCCGAGGAGG AGTACCTGGC CCAGTTGCAG GAAGCCAAGG AGTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010842716.1
CDS	1..1548
Translation	MAKHGAYAHQAGPSKSPGKAADPELLFPQVSLLCAQRLQTGRLHKVTGTDFPRSTWALTSASPDPSSPREEEETPLLSRAHLQAGSRRHRCRALPESAPLVLDKTPSPGTLKDAPLLLELQKLPGLTNTDLSALNPNIQMRATRAAMTQRIPGEKLGSTAQLRGEVDPQDELEVDLMAEPNNRWSEGAPCWLPAEELFWPLSWGYSEGEEERPRLKGRASGEEQEEEEEDDPPEYSEGEDQEDSEEHGRDDQEPGFSGAAGGWEQGPLSPRDWAFEEPDRYDYELQEEWSPWSLCSGTCGSGQQRTQPCSYTCIASKSCACDLPPSWSFCPQLGWADTSLVPLPPPLPSANVDSCEKWLNCKSDFLAKYLSQVLRALPSCPCAYALEAVSSAVSLWEEHQGRSFRWRDAGnullnullnullnullnullVPVHSPFCLRSLnullSTPAAQHCCYDVSSRLLTRGKGAGAPDLVSIDVSPELHFKVDTLPWIPCKADCTHYHAVRPANNGRACADNPPEEEYLAQLQEAKEY

Target ORF information:

RefSeq Version	XM_010844414.1
Organism	Bison bison bison
Definition	Bison bison bison isthmin 2 (ISM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010844414.1

ORF Insert Sequence:

ATGGCGAAAC ACGGCGCTTA TGCCCACCAG GCTGGGCCAT CTAAATCACC TGGAAAGGCT 
GCAGACCCTG AACTCCTGTT CCCCCAAGTC TCCCTTCTCT GTGCTCAGAG GCTGCAGACA 
GGCCGACTAC ACAAAGTCAC AGGGACAGAC TTTCCCAGGT CCACATGGGC CTTGACCTCA 
GCCTCCCCAG ATCCCAGCTC TCCCAGAGAA GAGGAGGAGA CCCCTCTGCT CTCCAGGGCC 
CACCTCCAGG CAGGGTCACG TCGACACAGA TGTCGGGCGC TCCCTGAATC AGCACCCCTG 
GTCCTAGATA AGACGCCCTC ACCTGGGACC CTGAAGGACG CTCCCTTGCT GCTGGAGCTG 
CAGAAATTGC CAGGATTGAC CAATACAGAT TTGAGTGCCT TGAACCCCAA CATCCAGATG 
AGGGCTACAA GGGCGGCCAT GACCCAGCGG ATTCCTGGAG AAAAGCTGGG CTCAACAGCT 
CAGCTCCGAG GTGAAGTGGA TCCCCAGGAT GAGCTGGAGG TGGACCTGAT GGCTGAGCCC 
AACAATCGCT GGTCCGAGGG CGCCCCCTGC TGGCTGCCTG CTGAGGAACT CTTCTGGCCT 
CTCTCTTGGG GCTACTCAGA GGGTGAGGAA GAGAGGCCCC GTCTCAAGGG CAGAGCCTCA 
GGAGAAGAGC AGGAGGAGGA AGAGGAGGAT GACCCCCCAG AGTACAGTGA GGGTGAGGAC 
CAGGAGGACA GTGAGGAACA CGGTAGAGAT GATCAGGAGC CGGGCTTCAG TGGGGCCGCA 
GGAGGCTGGG AGCAGGGCCC ACTGTCTCCC AGAGACTGGG CCTTTGAGGA GCCTGATCGC 
TATGACTATG AGCTTCAGGA GGAGTGGAGC CCCTGGTCTC TCTGCAGTGG GACCTGTGGC 
AGTGGCCAGC AGAGGACTCA GCCCTGCAGC TACACCTGCA TTGCCTCCAA GTCCTGCGCC 
TGCGACCTGC CCCCGTCCTG GTCGTTCTGC CCCCAGCTCG GGTGGGCTGA CACGTCCCTT 
GTTCCCCTGC CACCTCCCCT GCCCTCTGCA AATGTGGACA GCTGTGAAAA GTGGCTGAAC 
TGCAAGAGTG ACTTCCTGGC CAAGTACCTG AGCCAGGTGC TGCGGGCCCT GCCCAGCTGC 
CCATGCGCAT ACGCGTTGGA GGCCGTGTCC AGCGCCGTGA GCCTGTGGGA GGAGCATCAA 
GGCCGCAGCT TCCGCTGGAG GGATGCCGGC GGNNNNNNNN NNNNNGTGCC GGTCCACAGC 
CCCTTCTGCC TGCGCTCTCT GNNCAGCACG CCGGCAGCCC AGCACTGCTG CTACGACGTG 
AGCAGCCGGC TGCTGACCCG GGGCAAGGGT GCCGGCGCGC CCGACTTGGT CAGCATCGAC 
GTCTCGCCCG AGCTTCACTT CAAGGTGGAC ACGCTGCCCT GGATCCCGTG TAAGGCGGAC 
TGCACCCACT ACCACGCGGT GCGGCCCGCC AACAATGGCC GAGCCTGCGC GGACAACCCT 
CCCGAGGAGG AGTACCTGGC CCAGTTGCAG GAAGCCAAGG AGTACTAA