RPE65 cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	RPE65
Entrez Gene ID	104991595
Full Name	RPE65, retinoid isomerohydrolase
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010844136.1	XP_010842438.1	retinoid isomerohydrolase

Pathways from BioSystems
Gene Ontology

The following RPE65 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPE65 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi67882	XM_010844136.1 Latest version!	Bison bison bison retinal pigment epithelium-specific protein 65kDa (RPE65), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPE65 ( XM_010844136.1 ) cDNA ORF clone, Bison bison bison -> XP_010842438.1 Bison bison bison retinal pigment epithelium-specific protein 65kDa (RPE65), mRNA.

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CloneID	OBi67882
Clone ID Related Accession (Same CDS sequence)	XM_010844136.1
Accession Version	XM_010844136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	retinoid isomerohydrolase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494767.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCC AAGTTGAACA TCCAGCTGGT GGTTACAAGA AACTGTTTGA AACTGTGGAG 
GAACTATCCT CACCGCTCAC AGCCCATGTT ACAGGCAGGA TCCCCCTCTG GCTAACCGGC 
AGTCTCCTTC GATGTGGGCC AGGACTCTTT GAGGTTGGAT CGGAACCATT TTACCACCTG 
TTTGATGGGC AAGCCCTCCT ACACAAGTTT GACTTTAAAG AAGGACATGT CACATACCAC 
AGAAGGTTCA TCCGCACTGA TGCTTACGTA CGGGCAATGA CTGAGAAAAG GATCGTCATA 
ACAGAATTTG GCACCTGTGC TTTCCCAGAT CCCTGCAAGA ATATATTTTC CAGGTTTTTT 
TCTTACTTCC GAGGAGTGGA GGTTACTGAC AATGCCCTTG TTAATATCTA CCCAGTGGGG 
GAAGATTACT ATGCCTGCAC AGAGACCAAC TTCATTACAA AGATTAATCC TGAGACCTTG 
GAAACAATTA AGCAGGTTGA CCTTTGCAAC TATGTCTCAG TCAATGGAGC CACTGCTCAC 
CCCCACATTG AAAATGATGG GACTGTTTAC AACATTGGTA ATTGCTTTGG GAAAAATTTT 
TCAATTGCCT ACAATATTGT AAAGATCCCA CCACTACAAG CAGACAAGGA AGATCCAATA 
AGCAAGTCAG AGATCGTTGT ACAATTCCCC TGCAGTGACC GATTCAAGCC ATCTTACGTC 
CATAGTTTTG GTTTGACTCC CAACTATATT GTTTTTGTGG AGACACCAGT CAAAATTAAT 
CTGTTCAAGT TTCTTTCTTC ATGGAGTCTT TGGGGAGCCA ATTACATGGA TTGTTTTGAA 
TCCAATGAAA CCATGGGGGT TTGGCTTCAT ATTGCTGACA AAAAAAGAAA AAAGTATATC 
AATAATAAAT ACAGGACCTC TCCTTTTAAC CTTTTTCATC ACATCAATAC CTATGAAGAC 
CATGAGTTTC TGATTGTGGA TCTCTGTTGC TGGAAAGGAT TTGAATTTGT TTATAATTAT 
TTATATTTAG CCAATTTACG TGAGAACTGG GAAGAGGTGA AAAAAAATGC CAGAAAGGCT 
CCTCAGCCTG AAGTTAGGAG ATACGTACTT CCTTTGAATA TTGACAAGGC TGACACAGGC 
AAGAATTTAG TCACACTCCC CAACACAACT GCCACTGCAA TTCTGTGCAG TGACGAGACC 
ATCTGGCTGG AACCTGAGGT TCTCTTTTCA GGGCCTCGCC AAGCATTTGA GTTTCCTCAA 
ATCAATTACC AGAAGTATGG TGGGAAACCT TACACATATG CATATGGACT TGGCTTGAAT 
CACTTTGTTC CAGACAGGCT CTGTAAGCTG AACGTCAAAA CTAAAGAAAC CTGGGTATGG 
CAAGAGCCTG ATTCATACCC CTCAGAACCT ATCTTTGTTT CTCACCCAGA TGCCTTGGAG 
GAAGATGACG GTGTAGTTCT GAGTGTGGTG GTGAGCCCTG GGGCAGGACA AAAGCCTGCT 
TATCTTCTGA TTCTGAATGC CAAGGACTTG AGTGAAGTTG CCAGGGCTGA AGTGGAGATT 
AACATCCCCG TCACCTTTCA TGGACTGTTC AAAAAATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010842438.1
CDS	53..1654
Translation	MSSQVEHPAGGYKKLFETVEELSSPLTAHVTGRIPLWLTGSLLRCGPGLFEVGSEPFYHLFDGQALLHKFDFKEGHVTYHRRFIRTDAYVRAMTEKRIVITEFGTCAFPDPCKNIFSRFFSYFRGVEVTDNALVNIYPVGEDYYACTETNFITKINPETLETIKQVDLCNYVSVNGATAHPHIENDGTVYNIGNCFGKNFSIAYNIVKIPPLQADKEDPISKSEIVVQFPCSDRFKPSYVHSFGLTPNYIVFVETPVKINLFKFLSSWSLWGANYMDCFESNETMGVWLHIADKKRKKYINNKYRTSPFNLFHHINTYEDHEFLIVDLCCWKGFEFVYNYLYLANLRENWEEVKKNARKAPQPEVRRYVLPLNIDKADTGKNLVTLPNTTATAILCSDETIWLEPEVLFSGPRQAFEFPQINYQKYGGKPYTYAYGLGLNHFVPDRLCKLNVKTKETWVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPAYLLILNAKDLSEVARAEVEINIPVTFHGLFKKS

Target ORF information:

RefSeq Version	XM_010844136.1
Organism	Bison bison bison
Definition	Bison bison bison retinal pigment epithelium-specific protein 65kDa (RPE65), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010844136.1

ORF Insert Sequence:

ATGTCCAGCC AAGTTGAACA TCCAGCTGGT GGTTACAAGA AACTGTTTGA AACTGTGGAG 
GAACTATCCT CACCGCTCAC AGCCCATGTT ACAGGCAGGA TCCCCCTCTG GCTAACCGGC 
AGTCTCCTTC GATGTGGGCC AGGACTCTTT GAGGTTGGAT CGGAACCATT TTACCACCTG 
TTTGATGGGC AAGCCCTCCT ACACAAGTTT GACTTTAAAG AAGGACATGT CACATACCAC 
AGAAGGTTCA TCCGCACTGA TGCTTACGTA CGGGCAATGA CTGAGAAAAG GATCGTCATA 
ACAGAATTTG GCACCTGTGC TTTCCCAGAT CCCTGCAAGA ATATATTTTC CAGGTTTTTT 
TCTTACTTCC GAGGAGTGGA GGTTACTGAC AATGCCCTTG TTAATATCTA CCCAGTGGGG 
GAAGATTACT ATGCCTGCAC AGAGACCAAC TTCATTACAA AGATTAATCC TGAGACCTTG 
GAAACAATTA AGCAGGTTGA CCTTTGCAAC TATGTCTCAG TCAATGGAGC CACTGCTCAC 
CCCCACATTG AAAATGATGG GACTGTTTAC AACATTGGTA ATTGCTTTGG GAAAAATTTT 
TCAATTGCCT ACAATATTGT AAAGATCCCA CCACTACAAG CAGACAAGGA AGATCCAATA 
AGCAAGTCAG AGATCGTTGT ACAATTCCCC TGCAGTGACC GATTCAAGCC ATCTTACGTC 
CATAGTTTTG GTTTGACTCC CAACTATATT GTTTTTGTGG AGACACCAGT CAAAATTAAT 
CTGTTCAAGT TTCTTTCTTC ATGGAGTCTT TGGGGAGCCA ATTACATGGA TTGTTTTGAA 
TCCAATGAAA CCATGGGGGT TTGGCTTCAT ATTGCTGACA AAAAAAGAAA AAAGTATATC 
AATAATAAAT ACAGGACCTC TCCTTTTAAC CTTTTTCATC ACATCAATAC CTATGAAGAC 
CATGAGTTTC TGATTGTGGA TCTCTGTTGC TGGAAAGGAT TTGAATTTGT TTATAATTAT 
TTATATTTAG CCAATTTACG TGAGAACTGG GAAGAGGTGA AAAAAAATGC CAGAAAGGCT 
CCTCAGCCTG AAGTTAGGAG ATACGTACTT CCTTTGAATA TTGACAAGGC TGACACAGGC 
AAGAATTTAG TCACACTCCC CAACACAACT GCCACTGCAA TTCTGTGCAG TGACGAGACC 
ATCTGGCTGG AACCTGAGGT TCTCTTTTCA GGGCCTCGCC AAGCATTTGA GTTTCCTCAA 
ATCAATTACC AGAAGTATGG TGGGAAACCT TACACATATG CATATGGACT TGGCTTGAAT 
CACTTTGTTC CAGACAGGCT CTGTAAGCTG AACGTCAAAA CTAAAGAAAC CTGGGTATGG 
CAAGAGCCTG ATTCATACCC CTCAGAACCT ATCTTTGTTT CTCACCCAGA TGCCTTGGAG 
GAAGATGACG GTGTAGTTCT GAGTGTGGTG GTGAGCCCTG GGGCAGGACA AAAGCCTGCT 
TATCTTCTGA TTCTGAATGC CAAGGACTTG AGTGAAGTTG CCAGGGCTGA AGTGGAGATT 
AACATCCCCG TCACCTTTCA TGGACTGTTC AAAAAATCCT GA