RNF133 cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF133
Entrez Gene ID	104982377
Full Name	ring finger protein 133
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010831744.1	XP_010830046.1	E3 ubiquitin-protein ligase RNF133

Pathways from BioSystems
Gene Ontology

The following RNF133 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF133 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi84900	XM_010831744.1 Latest version!	Bison bison bison ring finger protein 133 (RNF133), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF133 ( XM_010831744.1 ) cDNA ORF clone, Bison bison bison -> XP_010830046.1 Bison bison bison ring finger protein 133 (RNF133), mRNA.

Price & Availability↑

CloneID	OBi84900
Clone ID Related Accession (Same CDS sequence)	XM_010831744.1
Accession Version	XM_010831744.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	E3 ubiquitin-protein ligase RNF133
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011495080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCTAC TCAAGGTTGG CACTCAGAGA AACAATGCTG CCTCTTCCTG GCTCATGAAA 
TTCAGCTTCC TTTGGCTGCT TAGCCAGCAC TGTTGCAGAA CCAGCGCTGT TTGGACTGCT 
TACATGAACA TATCGTTTCA TGTTGGGAAT CGCATGTTGT CAGAGTTGGG AGAGACTGGC 
GTGTTTGGAA GAAGCTCCAC TTTGAAGAGA GTGGCAGGAG TCATCGTGCC TCCAGAGGGA 
AAAACACAAA ATGCCTGCAG CCCCAATACC AGTTTCAGGA AGCTGAAGAA CTCGCAGACA 
TGGCTCGCTC TTGTCGAACG GGGAGGCTGT ACCTTCACCC AGAAGATCAA GGTGGCGGTG 
GAGAATGGAG CCAGTGGAGT GATCATCTAT AACTTTCCAG GAACTGGCAA TCAGGTCTTT 
CCCATGTCTC ATCAGATATT TGAAGACATC GTTGTGGTGA TGATTGGTAA CTTAAAAGGC 
ATGGAGATTT TGCATTTAAT TCAGAAGGGT GTTCATGTGA CCGTCACGAT TGAGGTGGGA 
AGAAAACACA TCATCTGGAT GAATCACTAT TTTGTCTCTT TTGTGATTGT CACAACCGCT 
ACGTTAGTGT ATTTCATCTT TTATCATATT CGAAGACTTT GGGTAGCAAG GGTTCAGAAC 
CGGAGGTGGC AGCGGTTAAC AAATGATCTC AAGAAAGCAT TTGGCCAGCT TCAGGTTCGG 
GTACTGAAAG AGGGGGACGA GGAAGTAAGC GGCAATGGAG ATAGCTGCGT AGTTTGCTTT 
GAACTCTATA AGCCTAACGA TACAATTCGT GTTCTGACCT GTAAACATTT TTTCCACAAG 
AATTGCATTG ACCCCTGGAT CTTAGCCCAT GGGACGTGCC CCATGTGCAA ATGTGATATT 
CTTAAAGCTT TGGGGATTCA GATGGATGTT GAAGATGGAA CAGAGTCCTT GCAAGTTTTA 
ATGTCCAACG AATTGGCTGA TATCCTGTCA TCTAGTGAAG AGGGCACAAA TAATGAACTT 
CCTCCTTCAG GAAGGTCAGA TAAAGTGGCA CACGAGGAGG AGGAGGAGCC CGTTTCTTCT 
CAGGACGTCG GCCACTCTAA TTCAGTAGCA GCGGACATCC ATCCTCCGCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010830046.1
CDS	169..1302
Translation	MHLLKVGTQRNNAASSWLMKFSFLWLLSQHCCRTSAVWTAYMNISFHVGNRMLSELGETGVFGRSSTLKRVAGVIVPPEGKTQNACSPNTSFRKLKNSQTWLALVERGGCTFTQKIKVAVENGASGVIIYNFPGTGNQVFPMSHQIFEDIVVVMIGNLKGMEILHLIQKGVHVTVTIEVGRKHIIWMNHYFVSFVIVTTATLVYFIFYHIRRLWVARVQNRRWQRLTNDLKKAFGQLQVRVLKEGDEEVSGNGDSCVVCFELYKPNDTIRVLTCKHFFHKNCIDPWILAHGTCPMCKCDILKALGIQMDVEDGTESLQVLMSNELADILSSSEEGTNNELPPSGRSDKVAHEEEEEPVSSQDVGHSNSVAADIHPPP

Target ORF information:

RefSeq Version	XM_010831744.1
Organism	Bison bison bison
Definition	Bison bison bison ring finger protein 133 (RNF133), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010831744.1

ORF Insert Sequence:

ATGCATCTAC TCAAGGTTGG CACTCAGAGA AACAATGCTG CCTCTTCCTG GCTCATGAAA 
TTCAGCTTCC TTTGGCTGCT TAGCCAGCAC TGTTGCAGAA CCAGCGCTGT TTGGACTGCT 
TACATGAACA TATCGTTTCA TGTTGGGAAT CGCATGTTGT CAGAGTTGGG AGAGACTGGC 
GTGTTTGGAA GAAGCTCCAC TTTGAAGAGA GTGGCAGGAG TCATCGTGCC TCCAGAGGGA 
AAAACACAAA ATGCCTGCAG CCCCAATACC AGTTTCAGGA AGCTGAAGAA CTCGCAGACA 
TGGCTCGCTC TTGTCGAACG GGGAGGCTGT ACCTTCACCC AGAAGATCAA GGTGGCGGTG 
GAGAATGGAG CCAGTGGAGT GATCATCTAT AACTTTCCAG GAACTGGCAA TCAGGTCTTT 
CCCATGTCTC ATCAGATATT TGAAGACATC GTTGTGGTGA TGATTGGTAA CTTAAAAGGC 
ATGGAGATTT TGCATTTAAT TCAGAAGGGT GTTCATGTGA CCGTCACGAT TGAGGTGGGA 
AGAAAACACA TCATCTGGAT GAATCACTAT TTTGTCTCTT TTGTGATTGT CACAACCGCT 
ACGTTAGTGT ATTTCATCTT TTATCATATT CGAAGACTTT GGGTAGCAAG GGTTCAGAAC 
CGGAGGTGGC AGCGGTTAAC AAATGATCTC AAGAAAGCAT TTGGCCAGCT TCAGGTTCGG 
GTACTGAAAG AGGGGGACGA GGAAGTAAGC GGCAATGGAG ATAGCTGCGT AGTTTGCTTT 
GAACTCTATA AGCCTAACGA TACAATTCGT GTTCTGACCT GTAAACATTT TTTCCACAAG 
AATTGCATTG ACCCCTGGAT CTTAGCCCAT GGGACGTGCC CCATGTGCAA ATGTGATATT 
CTTAAAGCTT TGGGGATTCA GATGGATGTT GAAGATGGAA CAGAGTCCTT GCAAGTTTTA 
ATGTCCAACG AATTGGCTGA TATCCTGTCA TCTAGTGAAG AGGGCACAAA TAATGAACTT 
CCTCCTTCAG GAAGGTCAGA TAAAGTGGCA CACGAGGAGG AGGAGGAGCC CGTTTCTTCT 
CAGGACGTCG GCCACTCTAA TTCAGTAGCA GCGGACATCC ATCCTCCGCC TTGA