RTCA cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	RTCA
Entrez Gene ID	104981728
Full Name	RNA 3'-terminal phosphate cyclase
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010830983.1	XP_010829285.1	LOW QUALITY PROTEIN: RNA 3'-terminal phosphate cyclase

Pathways from BioSystems
Gene Ontology

The following RTCA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RTCA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi84335	XM_010830983.1 Latest version!	Bison bison bison RNA 3'-terminal phosphate cyclase (RTCA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RTCA ( XM_010830983.1 ) cDNA ORF clone, Bison bison bison -> XP_010829285.1 Bison bison bison RNA 3'-terminal phosphate cyclase (RTCA), mRNA.

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CloneID	OBi84335
Clone ID Related Accession (Same CDS sequence)	XM_010830983.1
Accession Version	XM_010830983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	LOW QUALITY PROTEIN: RNA 3'-terminal phosphate cyclase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011495072.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCTGGGC CGCGGGTGGA GGGGGACGGC GGCATCATGG AAGGGGGCGG GCAGATCCTG 
AGGGTGTCTA CGGCCCTGAG CTGTCTGCTG GGCCTCCCTT TGCGGGTGCA GAAGATCCGA 
GCCGGCCGCA GCACGCCGGG CCTCAGGCCT CAACATTTAT CTGGACTGGA AATGATTCGA 
GATTTGTGTG ATGGGCAACT GGAGGGGGCA GAAATCGGCT CAACAGAAAT AACGTTTACA 
CCAGAGAAGA TCAAAGGTGG AGTCCACACA GCAGATACCA AGACAGCAGG GAGTGTCTGC 
CTCTTGATGC AGGTCTCAAT GCCCTGTGTT CTCTTTGCTG CTTGTCCATC AGAACTCCGT 
TTGAAAGGTG GGACTAATGC TGAAATGGCA CCACAGATTG ATTACACAGC CATGGTTTTC 
AAGCCAATTG TTGAAAAATT TGGTTTCACA TTTAATTGTG ACATTAAAAT GAGGGGCTAC 
TACCCAAAGG GAGGTGGTGA AGTGATTGTC CGAATGTCGC CAGTTAAACA GTTGAGCCCA 
ATAAATTTAA CTGACCGTGG CTGTGTGACT AAGATATATG GAAGAGCTTT TGTTGCTGGT 
GTTTTGCCAT TTAAAGTAGC AAAAGATATG GTGGCGGCAG CCGTAAGATG CATCAGGAAG 
GAGATTAGGG ATCTGTATGT TAACATCCAG CCTGTTCAGG AACCTAAAGA CCAAGCTTTT 
GGCAATGGAA ATGGAATAAT CATCATTGCC GAGACGTCCA CTGGCTGTCT GTTTGCTGGA 
TCGTCGCTTG GTAAACGAGG TGTTAATGCA GACAAGGTTG GAATTGAAGC TGCTGAAATG 
CTGTTAGCAA ATCTTAGACA TGGTGGTGCT GTGGATGAGT ATCTCCAAGA CCAGCTGATC 
ATTTTTATGG CATTAGCCAG TGGAATTTCC AGAATAAAAA CAGGACCAGT TACACTCCAC 
ACCCAAACAG CTATACATTT TGCTGAGCAA CTAGCAAAGG CTAAATTTAC TGTGAAGAAA 
TCAGAAGATG AAGAAGATGC CTCTAAAGAC ACTTATATTA TTGAATGCCA AGGAATTGGG 
ATGACAAATC CAAATCTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010829285.1
CDS	241..1341
Translation	MAGPRVEGDGGIMEGGGQILRVSTALSCLLGLPLRVQKIRAGRSTPGLRPQHLSGLEMIRDLCDGQLEGAEIGSTEITFTPEKIKGGVHTADTKTAGSVCLLMQVSMPCVLFAACPSELRLKGGTNAEMAPQIDYTAMVFKPIVEKFGFTFNCDIKMRGYYPKGGGEVIVRMSPVKQLSPINLTDRGCVTKIYGRAFVAGVLPFKVAKDMVAAAVRCIRKEIRDLYVNIQPVQEPKDQAFGNGNGIIIIAETSTGCLFAGSSLGKRGVNADKVGIEAAEMLLANLRHGGAVDEYLQDQLIIFMALASGISRIKTGPVTLHTQTAIHFAEQLAKAKFTVKKSEDEEDASKDTYIIECQGIGMTNPNL

Target ORF information:

RefSeq Version	XM_010830983.1
Organism	Bison bison bison
Definition	Bison bison bison RNA 3'-terminal phosphate cyclase (RTCA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010830983.1

ORF Insert Sequence:

ATGGCTGGGC CGCGGGTGGA GGGGGACGGC GGCATCATGG AAGGGGGCGG GCAGATCCTG 
AGGGTGTCTA CGGCCCTGAG CTGTCTGCTG GGCCTCCCTT TGCGGGTGCA GAAGATCCGA 
GCCGGCCGCA GCACGCCGGG CCTCAGGCCT CAACATTTAT CTGGACTGGA AATGATTCGA 
GATTTGTGTG ATGGGCAACT GGAGGGGGCA GAAATCGGCT CAACAGAAAT AACGTTTACA 
CCAGAGAAGA TCAAAGGTGG AGTCCACACA GCAGATACCA AGACAGCAGG GAGTGTCTGC 
CTCTTGATGC AGGTCTCAAT GCCCTGTGTT CTCTTTGCTG CTTGTCCATC AGAACTCCGT 
TTGAAAGGTG GGACTAATGC TGAAATGGCA CCACAGATTG ATTACACAGC CATGGTTTTC 
AAGCCAATTG TTGAAAAATT TGGTTTCACA TTTAATTGTG ACATTAAAAT GAGGGGCTAC 
TACCCAAAGG GAGGTGGTGA AGTGATTGTC CGAATGTCGC CAGTTAAACA GTTGAGCCCA 
ATAAATTTAA CTGACCGTGG CTGTGTGACT AAGATATATG GAAGAGCTTT TGTTGCTGGT 
GTTTTGCCAT TTAAAGTAGC AAAAGATATG GTGGCGGCAG CCGTAAGATG CATCAGGAAG 
GAGATTAGGG ATCTGTATGT TAACATCCAG CCTGTTCAGG AACCTAAAGA CCAAGCTTTT 
GGCAATGGAA ATGGAATAAT CATCATTGCC GAGACGTCCA CTGGCTGTCT GTTTGCTGGA 
TCGTCGCTTG GTAAACGAGG TGTTAATGCA GACAAGGTTG GAATTGAAGC TGCTGAAATG 
CTGTTAGCAA ATCTTAGACA TGGTGGTGCT GTGGATGAGT ATCTCCAAGA CCAGCTGATC 
ATTTTTATGG CATTAGCCAG TGGAATTTCC AGAATAAAAA CAGGACCAGT TACACTCCAC 
ACCCAAACAG CTATACATTT TGCTGAGCAA CTAGCAAAGG CTAAATTTAC TGTGAAGAAA 
TCAGAAGATG AAGAAGATGC CTCTAAAGAC ACTTATATTA TTGAATGCCA AGGAATTGGG 
ATGACAAATC CAAATCTATA G