NLRP3 cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane)

Gene
Clones
gRNAs

Summary

Gene Symbol	NLRP3
Entrez Gene ID	104633682
Full Name	NLR family pyrin domain containing 3
Gene Type	protein-coding
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010299966.1	XP_010298268.1	NACHT, LRR and PYD domains-containing protein 3

Pathways from BioSystems
Gene Ontology

The following NLRP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa182845	XM_010299966.1 Latest version!	Balearica regulorum gibbericeps NLR family, pyrin domain containing 3 (NLRP3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NLRP3 ( XM_010299966.1 ) cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane) -> XP_010298268.1 Balearica regulorum gibbericeps NLR family, pyrin domain containing 3 (NLRP3), mRNA.

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CloneID	OBa182845
Clone ID Related Accession (Same CDS sequence)	XM_010299966.1
Accession Version	XM_010299966.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-17
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Product	NACHT, LRR and PYD domains-containing protein 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010734293.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Balearica regulorum gibbericeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 10% of CDS bases ##RefSeq-Attributes-END##

ATGGCAAGAG AGAATACCAA AGACTTCCTT TTGGAAGCAC TGGAGGACCT TACGCTGGAT 
ATGTTTGAGA AGTTTAAGTA TAAATTATCA TATATCAATT ATGATGGGAT GGCCAATCTC 
TCCAAATATT TGCTCAAGAA AGCCAACAAT CCAGTTAAAC TTGCGGACTA CATGTGTGAC 
CACTATGGAG CCGACCTTGC TGTGGATGTA GCTATCTATG TGCTTGAATG GATCAACCAA 
AGAGACACTG CAGCTAAGCT CAAACAGGAG AAAGGAAAAG CCCTGAGGCC CAGTCCACCC 
CCTGCTTCAT TAGTCCAAGA TTACAAGATG AAGTACAGAG AACATGTACA AAAGAGTTAC 
CACTGGATAA AAGACATGAA TGCCCGTATT GGTGAGATGG TGACTCTGAA CTCCAGATAT 
ACAAGGCTGG TTCTTGTTAG TAGACATCGC CATGAAAAAA AGAAAGAACA TGAAATCATG 
GCTGTGGGAC AGAGACATGC AGAAATCATG CAGGAACAAG TGAATTCTTC CATTGCTATA 
AGTGATCTTT TTAAACCTGA CAGGGATAGA CAGACACCAA AGACTGTCGT GATATTGGGA 
GCTGCAGGAA TTGGGAAGAC AATGACAGTA AGAAAGATTA TGCTGGACTG GGCATCAGAA 
GGACTCTATA GACAGTTTGA CTATGTTTTC TACATACATT GTCGTGAGGG TAATCTTCTT 
CCAGCTCAGG GGAGTATGGC TGACATGATC TCGAAATGCT GTCCCAGTGA TAATCCACCT 
CTTGTAGAGA TCTTGAGGAC ACCAGAAAAA ATCTTGTTTG TCATCGATGG CTTTGATGAA 
CTGATGTTTT CTTTGGACCG GTCACAAAAT AACCTGTGCT CTGATCCCTG GGAGATGAAG 
CCAATGGAAA TCATCCTGAG CAGTTTATTT AGGAAAATCC TTCTCCCTGA ATGCTCCCTA 
TTGATAACAT CAAGACCAGC TGCCCTGCAG AAGCTAGAAC AGTGCTTGGT GTGTGAACGC 
TTTGCTGAAA TACTGGGATT TTCAGCAGCT GATAGGGAGG AATATTTCCA TAAGTTTTTT 
GAAAATAAGG AAGAGGGAAG GAAGGCCTTT CAGTTTGTTA AAGGAAATGA AATAGTCTTC 
ACCATGTGCC TTGTCCCCAT CATGTGCTGG ATCGTCTGTA CTGTTATGAA ACAACAGCTT 
GAAAGTGGTG AAGATCTTGC ACAAACTGTC AAGACAACTA CAGGAATATA TATCCTTTAT 
GTTTCCAGCT TACTAAAATC TCTGAGCAGC AAGATGAAAC AGAACTTGCA CATTATCCTG 
AAAAGACTTT GTTGCCTGGC TGTAGATGGG ATCTGGAAGC AAAGAGTCCT CTTTGAAGAG 
GAGGCAATCA ATGAATTCTT GTTAAATCAG AGAGACACTT TCCCACTTTT TTTGAATGAA 
AGCACTTTTC AGAAGGGCAT TGTTTGTGGG AGTGCCTACA GTTTCATTCA TCTGAGTGTC 
CAAGAGTTTT TTGCATCTCT ATTCTATGTC CTAGAAGATG ATGGCAAAAC AAGAGATAAG 
CAAGAAGATC CTAAGAGGGA TGTGAGAAAA TTGTTAGAAA ACTATGGAAA CTCAAGACAT 
GATTTCCTGT TAACTGTGAG GTTCCTATTT GGTCTCTTAA ATAAGGAACG AAGAAATGCT 
TTGGAAAAAG AAACAGGGTG CAAAATTAAT CCTGAAATTA AAGAAGAATT ACTAATGTGG 
CTTCGAACTA GCCAAAAAAC AGCTTTAGCT GTTAATGCAG AGAAAAGAGC AGTGATTCAC 
AATTTGGAGG CTTGCCACTG TTTGTATGAA ATCCAAGATG AAAGTTTTGT GAAAACTGCA 
TTGGGTTATT TCACTGGAAT ATATCTACGA GACATCAGTT TCACCCAACT AGATCAAATA 
GTTCTTTCAT TCAGTATAAA GAAGTGGCCT AAACTGGAGT CACTTGATGT GGGCTATTGC 
TCCTTTATCT CAGACGACCA TGAAGAACAT TTAGACCAAC AGCTGGCAAA GCTGTCACAT 
CTGTTGGAAT GGTGCAAACT CACAGCTGCC TGCTGTGGGG TTCTGGCCAC GGTGCTCATC 
ACCAGCCAGT GTCTGACGGA GCTGAGACTG TGTGGAAATG CTCTGGGAGA TCTAGGAGTG 
CGACTGCTGT GTGAAGGGCT GAAACATCCC ACCTGCCGGC TGCAGAGATT GAGGTTGTGG 
GGTTGCAGTC TCACAGATGC CTGCTGTGGG GATCTGGCCA ATATGCTCAG CACCAACCAG 
ACCCTGCTGG AGCTGGGGCT GGGCGCTAAT GCCCTGGGAG ATGCTGGAGT GCACCTGCTG 
TGTGAGGGGC TTAAACACCT TGCCTGCCAG CTGCGGGTAC TGGGGTTGTG GGGTTGCAGT 
CTCACAGATG CCTGCTGTGG GGATCTGGCC AATATGCTCA GCACCAACCA GACCCTGCTG 
GAGCTGGGGC TGGGCGCTAA TGCCCTGGGA GATGCTGGAG TGGTCCTGCT ATGTGAGAAG 
CTTAAACACC CTGCCTGCCA CCTTAAAGAA CTGCGGCTCA GCAAATACGG ACTCAGTGAA 
GAAACTCATC GGGAGCTGGC TCACCTAAAA AAGATCAAGC CAAGCCTGAA AATAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010298268.1
CDS	1..2640
Translation	MARENTKDFLLEALEDLTLDMFEKFKYKLSYINYDGMANLSKYLLKKANNPVKLADYMCDHYGADLAVDVAIYVLEWINQRDTAAKLKQEKGKALRPSPPPASLVQDYKMKYREHVQKSYHWIKDMNARIGEMVTLNSRYTRLVLVSRHRHEKKKEHEIMAVGQRHAEIMQEQVNSSIAISDLFKPDRDRQTPKTVVILGAAGIGKTMTVRKIMLDWASEGLYRQFDYVFYIHCREGNLLPAQGSMADMISKCCPSDNPPLVEILRTPEKILFVIDGFDELMFSLDRSQNNLCSDPWEMKPMEIILSSLFRKILLPECSLLITSRPAALQKLEQCLVCERFAEILGFSAADREEYFHKFFENKEEGRKAFQFVKGNEIVFTMCLVPIMCWIVCTVMKQQLESGEDLAQTVKTTTGIYILYVSSLLKSLSSKMKQNLHIILKRLCCLAVDGIWKQRVLFEEEAINEFLLNQRDTFPLFLNESTFQKGIVCGSAYSFIHLSVQEFFASLFYVLEDDGKTRDKQEDPKRDVRKLLENYGNSRHDFLLTVRFLFGLLNKERRNALEKETGCKINPEIKEELLMWLRTSQKTALAVNAEKRAVIHNLEACHCLYEIQDESFVKTALGYFTGIYLRDISFTQLDQIVLSFSIKKWPKLESLDVGYCSFISDDHEEHLDQQLAKLSHLLEWCKLTAACCGVLATVLITSQCLTELRLCGNALGDLGVRLLCEGLKHPTCRLQRLRLWGCSLTDACCGDLANMLSTNQTLLELGLGANALGDAGVHLLCEGLKHLACQLRVLGLWGCSLTDACCGDLANMLSTNQTLLELGLGANALGDAGVVLLCEKLKHPACHLKELRLSKYGLSEETHRELAHLKKIKPSLKIG

Target ORF information:

RefSeq Version	XM_010299966.1
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Definition	Balearica regulorum gibbericeps NLR family, pyrin domain containing 3 (NLRP3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010299966.1

ORF Insert Sequence:

ATGGCAAGAG AGAATACCAA AGACTTCCTT TTGGAAGCAC TGGAGGACCT TACGCTGGAT 
ATGTTTGAGA AGTTTAAGTA TAAATTATCA TATATCAATT ATGATGGGAT GGCCAATCTC 
TCCAAATATT TGCTCAAGAA AGCCAACAAT CCAGTTAAAC TTGCGGACTA CATGTGTGAC 
CACTATGGAG CCGACCTTGC TGTGGATGTA GCTATCTATG TGCTTGAATG GATCAACCAA 
AGAGACACTG CAGCTAAGCT CAAACAGGAG AAAGGAAAAG CCCTGAGGCC CAGTCCACCC 
CCTGCTTCAT TAGTCCAAGA TTACAAGATG AAGTACAGAG AACATGTACA AAAGAGTTAC 
CACTGGATAA AAGACATGAA TGCCCGTATT GGTGAGATGG TGACTCTGAA CTCCAGATAT 
ACAAGGCTGG TTCTTGTTAG TAGACATCGC CATGAAAAAA AGAAAGAACA TGAAATCATG 
GCTGTGGGAC AGAGACATGC AGAAATCATG CAGGAACAAG TGAATTCTTC CATTGCTATA 
AGTGATCTTT TTAAACCTGA CAGGGATAGA CAGACACCAA AGACTGTCGT GATATTGGGA 
GCTGCAGGAA TTGGGAAGAC AATGACAGTA AGAAAGATTA TGCTGGACTG GGCATCAGAA 
GGACTCTATA GACAGTTTGA CTATGTTTTC TACATACATT GTCGTGAGGG TAATCTTCTT 
CCAGCTCAGG GGAGTATGGC TGACATGATC TCGAAATGCT GTCCCAGTGA TAATCCACCT 
CTTGTAGAGA TCTTGAGGAC ACCAGAAAAA ATCTTGTTTG TCATCGATGG CTTTGATGAA 
CTGATGTTTT CTTTGGACCG GTCACAAAAT AACCTGTGCT CTGATCCCTG GGAGATGAAG 
CCAATGGAAA TCATCCTGAG CAGTTTATTT AGGAAAATCC TTCTCCCTGA ATGCTCCCTA 
TTGATAACAT CAAGACCAGC TGCCCTGCAG AAGCTAGAAC AGTGCTTGGT GTGTGAACGC 
TTTGCTGAAA TACTGGGATT TTCAGCAGCT GATAGGGAGG AATATTTCCA TAAGTTTTTT 
GAAAATAAGG AAGAGGGAAG GAAGGCCTTT CAGTTTGTTA AAGGAAATGA AATAGTCTTC 
ACCATGTGCC TTGTCCCCAT CATGTGCTGG ATCGTCTGTA CTGTTATGAA ACAACAGCTT 
GAAAGTGGTG AAGATCTTGC ACAAACTGTC AAGACAACTA CAGGAATATA TATCCTTTAT 
GTTTCCAGCT TACTAAAATC TCTGAGCAGC AAGATGAAAC AGAACTTGCA CATTATCCTG 
AAAAGACTTT GTTGCCTGGC TGTAGATGGG ATCTGGAAGC AAAGAGTCCT CTTTGAAGAG 
GAGGCAATCA ATGAATTCTT GTTAAATCAG AGAGACACTT TCCCACTTTT TTTGAATGAA 
AGCACTTTTC AGAAGGGCAT TGTTTGTGGG AGTGCCTACA GTTTCATTCA TCTGAGTGTC 
CAAGAGTTTT TTGCATCTCT ATTCTATGTC CTAGAAGATG ATGGCAAAAC AAGAGATAAG 
CAAGAAGATC CTAAGAGGGA TGTGAGAAAA TTGTTAGAAA ACTATGGAAA CTCAAGACAT 
GATTTCCTGT TAACTGTGAG GTTCCTATTT GGTCTCTTAA ATAAGGAACG AAGAAATGCT 
TTGGAAAAAG AAACAGGGTG CAAAATTAAT CCTGAAATTA AAGAAGAATT ACTAATGTGG 
CTTCGAACTA GCCAAAAAAC AGCTTTAGCT GTTAATGCAG AGAAAAGAGC AGTGATTCAC 
AATTTGGAGG CTTGCCACTG TTTGTATGAA ATCCAAGATG AAAGTTTTGT GAAAACTGCA 
TTGGGTTATT TCACTGGAAT ATATCTACGA GACATCAGTT TCACCCAACT AGATCAAATA 
GTTCTTTCAT TCAGTATAAA GAAGTGGCCT AAACTGGAGT CACTTGATGT GGGCTATTGC 
TCCTTTATCT CAGACGACCA TGAAGAACAT TTAGACCAAC AGCTGGCAAA GCTGTCACAT 
CTGTTGGAAT GGTGCAAACT CACAGCTGCC TGCTGTGGGG TTCTGGCCAC GGTGCTCATC 
ACCAGCCAGT GTCTGACGGA GCTGAGACTG TGTGGAAATG CTCTGGGAGA TCTAGGAGTG 
CGACTGCTGT GTGAAGGGCT GAAACATCCC ACCTGCCGGC TGCAGAGATT GAGGTTGTGG 
GGTTGCAGTC TCACAGATGC CTGCTGTGGG GATCTGGCCA ATATGCTCAG CACCAACCAG 
ACCCTGCTGG AGCTGGGGCT GGGCGCTAAT GCCCTGGGAG ATGCTGGAGT GCACCTGCTG 
TGTGAGGGGC TTAAACACCT TGCCTGCCAG CTGCGGGTAC TGGGGTTGTG GGGTTGCAGT 
CTCACAGATG CCTGCTGTGG GGATCTGGCC AATATGCTCA GCACCAACCA GACCCTGCTG 
GAGCTGGGGC TGGGCGCTAA TGCCCTGGGA GATGCTGGAG TGGTCCTGCT ATGTGAGAAG 
CTTAAACACC CTGCCTGCCA CCTTAAAGAA CTGCGGCTCA GCAAATACGG ACTCAGTGAA 
GAAACTCATC GGGAGCTGGC TCACCTAAAA AAGATCAAGC CAAGCCTGAA AATAGGATGA