DDX52 cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX52
Entrez Gene ID	104632621
Full Name	DExD-box helicase 52
Gene Type	protein-coding
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010298531.1	XP_010296833.1	probable ATP-dependent RNA helicase DDX52 isoform X1
XM_010298532.1	XP_010296834.1	probable ATP-dependent RNA helicase DDX52 isoform X2

Pathways from BioSystems
Gene Ontology

The following DDX52 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX52 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa184755	XM_010298531.1 Latest version!	Balearica regulorum gibbericeps DEAD (Asp-Glu-Ala-Asp) box polypeptide 52 (DDX52), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OBa184756	XM_010298532.1 Latest version!	Balearica regulorum gibbericeps DEAD (Asp-Glu-Ala-Asp) box polypeptide 52 (DDX52), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX52 ( XM_010298531.1 ) cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane) -> XP_010296833.1 Balearica regulorum gibbericeps DEAD (Asp-Glu-Ala-Asp) box polypeptide 52 (DDX52), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa184755
Clone ID Related Accession (Same CDS sequence)	XM_010298531.1
Accession Version	XM_010298531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-17
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Product	probable ATP-dependent RNA helicase DDX52 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010745228.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Balearica regulorum gibbericeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA AGAGAAAAAG AACTGCAGTG AGCAGTGAAA GGATGAAAAA AAAGAAAAAG 
AAACGAGAGG CAGCATCAAT GCCAGAGTTG TCAGAAGGTA ATGGAATAAA GTGGATGTCC 
TCTCTGGAAG CAAAATTCGA AGATGTAAAA GACAAAAAAC CTACTGCAGA AAAACTTGAA 
CGCTTGAGAA GAGAAAAGAT AAACCGCTTC AGAAACCAAC ACAAGATTAC TATTCAAGGA 
ACAGATCTTC CTGACCCAAT TGCCACGTTT GATCAACTTC AAAGGGAATA CAAAATCCAC 
CCGAAAATCA TGGAGAATAT TCAAGCTGCT GGCTTCCAGG TCCCAACACC CATCCAGATG 
CAAGCTATTC CCGTCATGCT TCATGGTCGG GAACTTCTAG CTTCAGCTCC TACTGGGTCT 
GGAAAAACAC TGGCATTCTG TATTCCTCTC TTAGCACACC TGAAACAGCC TAGGAACAAA 
GGATTCAGAG CTCTGATCAT ATCACCTACG CGAGAACTTG CCAGCCAGAC ACATCGGGAA 
CTGCTGAAGT TGGCCGAGGG GACAGGCTTC AGAATACATA TGATCAACAA GGCAGCTGAA 
GCAGCAAAGA AATTTGGGCC CAAGTCTTCT AAGAAATTTG ATATACTAGT TACTACTCCA 
AACAGGCTCA TTTATTTGCT GAAACAAGAT CCTCCAGCGA TAGACTTGAC CAGTGTGGAG 
TGGTTGGTGG TGGATGAGTC AGACAAACTG TTTGAAGATG GAAAATCGGG GTTCCGAGAC 
CAGCTGGCCT CCATCTTCCT GGCATGCACA TCCCACATGG TGAGAAGAGC TTTGTTCAGC 
GCAACCTTTG CACGTGATGT AGAGGAGTGG TGTAAACTCA ACCTTGACAG TGTTGTTCTG 
GTGTCCGTGG GAGCACGAAA CTCTGCAGCG GAGACAGTAG AACAAGAGCT GTTGTTTGTT 
GGATCTGAGA CAGGAAAACT AACAGCAATG CGAGAGCTCA TTAAAAAGGG TTTTGCTCCT 
CCAGTCCTTG TTTTTGTACA GTCTATTGAG AGGGCTAAAG AGCTTTTCCA TGAACTTATT 
TACGAAGGCA TCAACGTGGA TGTCATCCAT GCAGACAAAA CTCAGCAACA GAGAGATAAT 
GTAGTACACA GTTTCAGAGC TGGGAAGATC TGGGTGCTTA TCTGCTCAGC CTTGCTAGCT 
CGAGGGATTG ACTTCAAAGG AGTGAATATG GTCATTAATT ACGATTTGCC AACGAGTGCA 
GTGGAATACA TCCACAGGAT AGGTCGTACT GGAAGAGCAG GGCACACAGG AAAAGCAGTC 
ACTTTCTTTA CAGAAGACGA TAAACCTTTA TTAAGGAGTA TAGCCAATGT GATTCAGCGA 
GCTGGCTGTC CTGTGCCAGA CTACATAAAA CACTTTCCCA AACTGCAAAG CAAACAAAAG 
AAGAAGTTCA TTAAGAAACC ACTGACAAGA GAATCCATTT GTACCACTCC TCAGTGCTTC 
TTAAAAAAGA CCAAAAGAAA AATGAAAACT ACAAAGGAAA ATATTAAGGA GAAAAAGAAA 
GTTAAAGAAG CTAAAAATAG CAATAACTTA CAGACTGTTT CAAAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010296833.1
CDS	106..1716
Translation	MAGKRKRTAVSSERMKKKKKKREAASMPELSEGNGIKWMSSLEAKFEDVKDKKPTAEKLERLRREKINRFRNQHKITIQGTDLPDPIATFDQLQREYKIHPKIMENIQAAGFQVPTPIQMQAIPVMLHGRELLASAPTGSGKTLAFCIPLLAHLKQPRNKGFRALIISPTRELASQTHRELLKLAEGTGFRIHMINKAAEAAKKFGPKSSKKFDILVTTPNRLIYLLKQDPPAIDLTSVEWLVVDESDKLFEDGKSGFRDQLASIFLACTSHMVRRALFSATFARDVEEWCKLNLDSVVLVSVGARNSAAETVEQELLFVGSETGKLTAMRELIKKGFAPPVLVFVQSIERAKELFHELIYEGINVDVIHADKTQQQRDNVVHSFRAGKIWVLICSALLARGIDFKGVNMVINYDLPTSAVEYIHRIGRTGRAGHTGKAVTFFTEDDKPLLRSIANVIQRAGCPVPDYIKHFPKLQSKQKKKFIKKPLTRESICTTPQCFLKKTKRKMKTTKENIKEKKKVKEAKNSNNLQTVSKS

Target ORF information:

RefSeq Version	XM_010298531.1
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Definition	Balearica regulorum gibbericeps DEAD (Asp-Glu-Ala-Asp) box polypeptide 52 (DDX52), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010298531.1

ORF Insert Sequence:

ATGGCAGGAA AGAGAAAAAG AACTGCAGTG AGCAGTGAAA GGATGAAAAA AAAGAAAAAG 
AAACGAGAGG CAGCATCAAT GCCAGAGTTG TCAGAAGGTA ATGGAATAAA GTGGATGTCC 
TCTCTGGAAG CAAAATTCGA AGATGTAAAA GACAAAAAAC CTACTGCAGA AAAACTTGAA 
CGCTTGAGAA GAGAAAAGAT AAACCGCTTC AGAAACCAAC ACAAGATTAC TATTCAAGGA 
ACAGATCTTC CTGACCCAAT TGCCACGTTT GATCAACTTC AAAGGGAATA CAAAATCCAC 
CCGAAAATCA TGGAGAATAT TCAAGCTGCT GGCTTCCAGG TCCCAACACC CATCCAGATG 
CAAGCTATTC CCGTCATGCT TCATGGTCGG GAACTTCTAG CTTCAGCTCC TACTGGGTCT 
GGAAAAACAC TGGCATTCTG TATTCCTCTC TTAGCACACC TGAAACAGCC TAGGAACAAA 
GGATTCAGAG CTCTGATCAT ATCACCTACG CGAGAACTTG CCAGCCAGAC ACATCGGGAA 
CTGCTGAAGT TGGCCGAGGG GACAGGCTTC AGAATACATA TGATCAACAA GGCAGCTGAA 
GCAGCAAAGA AATTTGGGCC CAAGTCTTCT AAGAAATTTG ATATACTAGT TACTACTCCA 
AACAGGCTCA TTTATTTGCT GAAACAAGAT CCTCCAGCGA TAGACTTGAC CAGTGTGGAG 
TGGTTGGTGG TGGATGAGTC AGACAAACTG TTTGAAGATG GAAAATCGGG GTTCCGAGAC 
CAGCTGGCCT CCATCTTCCT GGCATGCACA TCCCACATGG TGAGAAGAGC TTTGTTCAGC 
GCAACCTTTG CACGTGATGT AGAGGAGTGG TGTAAACTCA ACCTTGACAG TGTTGTTCTG 
GTGTCCGTGG GAGCACGAAA CTCTGCAGCG GAGACAGTAG AACAAGAGCT GTTGTTTGTT 
GGATCTGAGA CAGGAAAACT AACAGCAATG CGAGAGCTCA TTAAAAAGGG TTTTGCTCCT 
CCAGTCCTTG TTTTTGTACA GTCTATTGAG AGGGCTAAAG AGCTTTTCCA TGAACTTATT 
TACGAAGGCA TCAACGTGGA TGTCATCCAT GCAGACAAAA CTCAGCAACA GAGAGATAAT 
GTAGTACACA GTTTCAGAGC TGGGAAGATC TGGGTGCTTA TCTGCTCAGC CTTGCTAGCT 
CGAGGGATTG ACTTCAAAGG AGTGAATATG GTCATTAATT ACGATTTGCC AACGAGTGCA 
GTGGAATACA TCCACAGGAT AGGTCGTACT GGAAGAGCAG GGCACACAGG AAAAGCAGTC 
ACTTTCTTTA CAGAAGACGA TAAACCTTTA TTAAGGAGTA TAGCCAATGT GATTCAGCGA 
GCTGGCTGTC CTGTGCCAGA CTACATAAAA CACTTTCCCA AACTGCAAAG CAAACAAAAG 
AAGAAGTTCA TTAAGAAACC ACTGACAAGA GAATCCATTT GTACCACTCC TCAGTGCTTC 
TTAAAAAAGA CCAAAAGAAA AATGAAAACT ACAAAGGAAA ATATTAAGGA GAAAAAGAAA 
GTTAAAGAAG CTAAAAATAG CAATAACTTA CAGACTGTTT CAAAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX52 ( XM_010298532.1 ) cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane) -> XP_010296834.1 Balearica regulorum gibbericeps DEAD (Asp-Glu-Ala-Asp) box polypeptide 52 (DDX52), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa184756
Clone ID Related Accession (Same CDS sequence)	XM_010298532.1
Accession Version	XM_010298532.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-17
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Product	probable ATP-dependent RNA helicase DDX52 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010745228.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Balearica regulorum gibbericeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA AGAGAAAAAG AACTGCAGTG AGCAGTGAAA GGATGAAAAA AAAGAAAAAG 
AAACGAGCAT CAATGCCAGA GTTGTCAGAA GGTAATGGAA TAAAGTGGAT GTCCTCTCTG 
GAAGCAAAAT TCGAAGATGT AAAAGACAAA AAACCTACTG CAGAAAAACT TGAACGCTTG 
AGAAGAGAAA AGATAAACCG CTTCAGAAAC CAACACAAGA TTACTATTCA AGGAACAGAT 
CTTCCTGACC CAATTGCCAC GTTTGATCAA CTTCAAAGGG AATACAAAAT CCACCCGAAA 
ATCATGGAGA ATATTCAAGC TGCTGGCTTC CAGGTCCCAA CACCCATCCA GATGCAAGCT 
ATTCCCGTCA TGCTTCATGG TCGGGAACTT CTAGCTTCAG CTCCTACTGG GTCTGGAAAA 
ACACTGGCAT TCTGTATTCC TCTCTTAGCA CACCTGAAAC AGCCTAGGAA CAAAGGATTC 
AGAGCTCTGA TCATATCACC TACGCGAGAA CTTGCCAGCC AGACACATCG GGAACTGCTG 
AAGTTGGCCG AGGGGACAGG CTTCAGAATA CATATGATCA ACAAGGCAGC TGAAGCAGCA 
AAGAAATTTG GGCCCAAGTC TTCTAAGAAA TTTGATATAC TAGTTACTAC TCCAAACAGG 
CTCATTTATT TGCTGAAACA AGATCCTCCA GCGATAGACT TGACCAGTGT GGAGTGGTTG 
GTGGTGGATG AGTCAGACAA ACTGTTTGAA GATGGAAAAT CGGGGTTCCG AGACCAGCTG 
GCCTCCATCT TCCTGGCATG CACATCCCAC ATGGTGAGAA GAGCTTTGTT CAGCGCAACC 
TTTGCACGTG ATGTAGAGGA GTGGTGTAAA CTCAACCTTG ACAGTGTTGT TCTGGTGTCC 
GTGGGAGCAC GAAACTCTGC AGCGGAGACA GTAGAACAAG AGCTGTTGTT TGTTGGATCT 
GAGACAGGAA AACTAACAGC AATGCGAGAG CTCATTAAAA AGGGTTTTGC TCCTCCAGTC 
CTTGTTTTTG TACAGTCTAT TGAGAGGGCT AAAGAGCTTT TCCATGAACT TATTTACGAA 
GGCATCAACG TGGATGTCAT CCATGCAGAC AAAACTCAGC AACAGAGAGA TAATGTAGTA 
CACAGTTTCA GAGCTGGGAA GATCTGGGTG CTTATCTGCT CAGCCTTGCT AGCTCGAGGG 
ATTGACTTCA AAGGAGTGAA TATGGTCATT AATTACGATT TGCCAACGAG TGCAGTGGAA 
TACATCCACA GGATAGGTCG TACTGGAAGA GCAGGGCACA CAGGAAAAGC AGTCACTTTC 
TTTACAGAAG ACGATAAACC TTTATTAAGG AGTATAGCCA ATGTGATTCA GCGAGCTGGC 
TGTCCTGTGC CAGACTACAT AAAACACTTT CCCAAACTGC AAAGCAAACA AAAGAAGAAG 
TTCATTAAGA AACCACTGAC AAGAGAATCC ATTTGTACCA CTCCTCAGTG CTTCTTAAAA 
AAGACCAAAA GAAAAATGAA AACTACAAAG GAAAATATTA AGGAGAAAAA GAAAGTTAAA 
GAAGCTAAAA ATAGCAATAA CTTACAGACT GTTTCAAAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010296834.1
CDS	31..1635
Translation	MAGKRKRTAVSSERMKKKKKKRASMPELSEGNGIKWMSSLEAKFEDVKDKKPTAEKLERLRREKINRFRNQHKITIQGTDLPDPIATFDQLQREYKIHPKIMENIQAAGFQVPTPIQMQAIPVMLHGRELLASAPTGSGKTLAFCIPLLAHLKQPRNKGFRALIISPTRELASQTHRELLKLAEGTGFRIHMINKAAEAAKKFGPKSSKKFDILVTTPNRLIYLLKQDPPAIDLTSVEWLVVDESDKLFEDGKSGFRDQLASIFLACTSHMVRRALFSATFARDVEEWCKLNLDSVVLVSVGARNSAAETVEQELLFVGSETGKLTAMRELIKKGFAPPVLVFVQSIERAKELFHELIYEGINVDVIHADKTQQQRDNVVHSFRAGKIWVLICSALLARGIDFKGVNMVINYDLPTSAVEYIHRIGRTGRAGHTGKAVTFFTEDDKPLLRSIANVIQRAGCPVPDYIKHFPKLQSKQKKKFIKKPLTRESICTTPQCFLKKTKRKMKTTKENIKEKKKVKEAKNSNNLQTVSKS

Target ORF information:

RefSeq Version	XM_010298532.1
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Definition	Balearica regulorum gibbericeps DEAD (Asp-Glu-Ala-Asp) box polypeptide 52 (DDX52), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010298532.1

ORF Insert Sequence:

ATGGCAGGAA AGAGAAAAAG AACTGCAGTG AGCAGTGAAA GGATGAAAAA AAAGAAAAAG 
AAACGAGCAT CAATGCCAGA GTTGTCAGAA GGTAATGGAA TAAAGTGGAT GTCCTCTCTG 
GAAGCAAAAT TCGAAGATGT AAAAGACAAA AAACCTACTG CAGAAAAACT TGAACGCTTG 
AGAAGAGAAA AGATAAACCG CTTCAGAAAC CAACACAAGA TTACTATTCA AGGAACAGAT 
CTTCCTGACC CAATTGCCAC GTTTGATCAA CTTCAAAGGG AATACAAAAT CCACCCGAAA 
ATCATGGAGA ATATTCAAGC TGCTGGCTTC CAGGTCCCAA CACCCATCCA GATGCAAGCT 
ATTCCCGTCA TGCTTCATGG TCGGGAACTT CTAGCTTCAG CTCCTACTGG GTCTGGAAAA 
ACACTGGCAT TCTGTATTCC TCTCTTAGCA CACCTGAAAC AGCCTAGGAA CAAAGGATTC 
AGAGCTCTGA TCATATCACC TACGCGAGAA CTTGCCAGCC AGACACATCG GGAACTGCTG 
AAGTTGGCCG AGGGGACAGG CTTCAGAATA CATATGATCA ACAAGGCAGC TGAAGCAGCA 
AAGAAATTTG GGCCCAAGTC TTCTAAGAAA TTTGATATAC TAGTTACTAC TCCAAACAGG 
CTCATTTATT TGCTGAAACA AGATCCTCCA GCGATAGACT TGACCAGTGT GGAGTGGTTG 
GTGGTGGATG AGTCAGACAA ACTGTTTGAA GATGGAAAAT CGGGGTTCCG AGACCAGCTG 
GCCTCCATCT TCCTGGCATG CACATCCCAC ATGGTGAGAA GAGCTTTGTT CAGCGCAACC 
TTTGCACGTG ATGTAGAGGA GTGGTGTAAA CTCAACCTTG ACAGTGTTGT TCTGGTGTCC 
GTGGGAGCAC GAAACTCTGC AGCGGAGACA GTAGAACAAG AGCTGTTGTT TGTTGGATCT 
GAGACAGGAA AACTAACAGC AATGCGAGAG CTCATTAAAA AGGGTTTTGC TCCTCCAGTC 
CTTGTTTTTG TACAGTCTAT TGAGAGGGCT AAAGAGCTTT TCCATGAACT TATTTACGAA 
GGCATCAACG TGGATGTCAT CCATGCAGAC AAAACTCAGC AACAGAGAGA TAATGTAGTA 
CACAGTTTCA GAGCTGGGAA GATCTGGGTG CTTATCTGCT CAGCCTTGCT AGCTCGAGGG 
ATTGACTTCA AAGGAGTGAA TATGGTCATT AATTACGATT TGCCAACGAG TGCAGTGGAA 
TACATCCACA GGATAGGTCG TACTGGAAGA GCAGGGCACA CAGGAAAAGC AGTCACTTTC 
TTTACAGAAG ACGATAAACC TTTATTAAGG AGTATAGCCA ATGTGATTCA GCGAGCTGGC 
TGTCCTGTGC CAGACTACAT AAAACACTTT CCCAAACTGC AAAGCAAACA AAAGAAGAAG 
TTCATTAAGA AACCACTGAC AAGAGAATCC ATTTGTACCA CTCCTCAGTG CTTCTTAAAA 
AAGACCAAAA GAAAAATGAA AACTACAAAG GAAAATATTA AGGAGAAAAA GAAAGTTAAA 
GAAGCTAAAA ATAGCAATAA CTTACAGACT GTTTCAAAAA GCTGA