F751_5926 cDNA ORF clone, Auxenochlorella protothecoides

Gene
Clones
gRNAs

Summary

Gene Symbol	F751_5926
Entrez Gene ID	23617317
Full Name	Sn1-specific diacylglycerol lipase alpha
Gene Type	protein-coding
Organism	Auxenochlorella protothecoides

mRNA and Protein(s)

mRNA	Protein	Name
XM_011402506.1	XP_011400808.1	Sn1-specific diacylglycerol lipase alpha

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Oil accumulation mechanisms of the oleaginous microalga Chlorella protothecoides revealed through its genome, transcriptomes, and proteomes.
Gao C, Wang Y, Shen Y, Yan D, He X, Dai J, Wu Q
BMC genomics15582(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following F751_5926 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F751_5926 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAu63907	XM_011402506.1 Latest version!	Auxenochlorella protothecoides Sn1-specific diacylglycerol lipase alpha partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F751_5926 ( XM_011402506.1 ) cDNA ORF clone, Auxenochlorella protothecoides -> XP_011400808.1 Auxenochlorella protothecoides Sn1-specific diacylglycerol lipase alpha partial mRNA.

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CloneID	OAu63907
Clone ID Related Accession (Same CDS sequence)	XM_011402506.1
Accession Version	XM_011402506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-23
Organism	Auxenochlorella protothecoides
Product	Sn1-specific diacylglycerol lipase alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_011934361). COMPLETENESS: incomplete on both ends.

ATGCCTGCCG TCCGGCTTTG GGGCCGGAGG TGGCACTTCA GCACCGACAT CGTTCCCATC 
CCCGCTGCCA TCAACGGCGC CTTCCATTTT GTCTGGATAT GCGTTCTCCT CTTCGGTGCT 
ATTGGATCAG GGGAATGGCC CAGTGGGTGC AATAGCGCGA CTGGGCGGCA GTACGTGGCG 
TTGTTCGCCG GCCTGTTTGG GGCCTTCGTT CTTAATCTCA TCATCGACGT CCTGCTCTAC 
ATCCATGGAA TGAAGGGATC TCCATTCGAG GTGTCCAAAC GGCACTGGGT TCCGACCCTC 
CTCCACATCA GCACTCTACC CATTGTCGCT CAGTTCGTGT TCACAGCATT TGGGACCCAT 
GTCGTGAATC AGCAGCTGGA CGACTGCTGG CCGGCAGACG TCAGGCATGG CGTGAGGGTG 
GTGGCGCGGA TCCTCGTCTA CTCCACCTGG GGCCTGATGG CCATCATCGC GCTGGGAATC 
GCCATCACGT ACAACCTGTA CCCGGAGCAC AAGTCGCAGG GTTCCTGGGA GCAGCGGTGC 
GAGTGCATCG CCGTGCTGCT GTGCTGCCGC AGCAACATGC GGCGCCGGCC AGCGGGCAAG 
CGCCCCCCGC TGTCCAACAT CGCGGAGCTG ACCTCCGGCC TGCTGTCGCA CGTAGACCTG 
GAGGCCACCG ACCTGATGAC GGCCGTGATC CTGACCTCCG CCGCGCAAAA CCGGCGGCGG 
CGCCTGCGCC TGGCCAAGGC GCTGCTGCCC GTGTACGAAG CGCTGCGCCA GACGGGGTCG 
CGGAGCAGCC GGTCGGATCC CGGCACGGCC GCCAGCTCCG CCGGCGACTC CGACTCGGAC 
TCCGAGGCGG AGAGCGGGGA TGAGGCGGGG GCCAGCAGCG ACGGCTCCTC GGTGCATGAC 
GTGCCAGCCA GCCGCCGTCT GACGCGGCGG CTGGAGGGCG GGCACTCGGA GCGGGGCAGG 
GCTGCGGCGC CCCGTGGGGA GCCGAGGCCA GAGGCGCCGG AGGTCGCGAT CGACACCGCC 
GCCAGCCTGC CCCTGACCGA GAGCAACGTG GCGCTGATGG ATCGGCTGGC CAGCGCCTCG 
GTGGGCGGCG AGGAAAGCGC GGCGGGGGGC GGAGACGACG GGAAGCAGGA GGCGGCGGCG 
GTGGCAGTGA CCAACGAGCT CCACGACCTG GCTGCCAACA CGGGGCAAGA CGCGGGGGAA 
TTGGCCGATG CACTGGCGGA TGCCATCCAG GATGAGCTGG AGGATCTGGG TGGCGGGGAC 
ATCGGGGCCA CCCACGAGGA CGCGGTTCTC CAAGTGGACA AGGCCCCCTC GCTGGATCTG 
GCCCTGCTGG CAGACGGCGC CTCCTCCATC TGGCTGAACC CCAGCGTCGC AACGCAGGCC 
CCCTCCGTCT CCAATCAGGG CGAGGAGACG GCACCCGGCT CTCCCCGCCG GACCCAGGAC 
GCCGAGTTCC CGGCCGGAGT CTCTGACTCC GGGCCCCCAG ACTCGCCATC AGTGCCCCCC 
GCGACGGGAA GCAGCCCCTC CACCCAGCCC CCGCCGGTGA CGGTGCCGGA GGGCGTGGAC 
CCGCTGCCCT CCCGCGAGGC CGTGGTGGCG GCCAGGCAGG CCGGCGCGCC GGGGTCGGAG 
GGCCGCCTGC CCTCCGAGGC CAGCCAGGGG GCGCGCAGCG GCAGCGTGCG CGACCTGGCC 
AGCGTTACGC TGCGCTTCCC CACCGTCATC ACGCCTTCCC AGTACGTCAA CCGCCTGGCA 
GGGAAGCTGC CGCCCGAGGC GCTGGCCGAC ATCTACGCTG GCCGGCATGA CCGCGTGGGG 
GTGGAGGTCC TGGAGGAGGC CACCTACTTC CTCAAGTACG CCTACGCTGT CTACTCCCTG 
CAGCCCAAGA TCGAGAACCC CTCCAGCATC CTGGACATGG CCTGCTGGAA GCCGCCCGCG 
CCCCAGGACG TGGTTTTTTC AGGGTTCAAT GAGCTGGAGC AACTGGAGGA TGACTCCACC 
ATCGAGCTGC TGCACCTCAA CTGCTCCAAC CGGATGCTGG CGCACCTGCC CTACCTCATC 
GCTCTGGACC ATGTGAAGAA GGCGGTGGTG CTGGCCATCA GGGGTACCAT CTCTGCCGCC 
GACATTGTGA CGGATGCCAT GGTCGCCAGC GAGCGCCTGG AGCACCTGCT CCCTGTCAGC 
CTGAAGGAGG ACGTCAAGGG CCCCTGCTTC GGGCACACGG GCATGCTGGC GGCGGCCCAG 
GCCATCTTCA TGGACATGAA GGAGCGGGGC ATCCTGACCC CGCTAGTGGG CAGCGAGGCG 
GCGGCTGACG ACTACTCCGC GCCGCGCGGG GAGGGCCCGC CCCAGCGGGA ATCCCAGTAC 
GACGCCGACA CGGGGCCCAG CCCCCTGCGC GCGGCCATGG GCAGCGCGGG GCAGGCCATT 
CGCGAGGGCG TCGCGGCGGT GCTGCCCGCG GGGGGCTGGA CAGGGGCGGG CAGGCCAGGG 
GATGCCGAAG GGGACGACAT AGAGGCACAG CGCGCGGCGG CGAGAGATTC CGACGGACCA 
CGTGTCACGG TGGACAAGCT GGGGGCGCAA AAGTCGCCGG GCCAGAAGGT GGGGGAGCTG 
ATGAAGGTGA AGCTGCTGGA GGACGACTGG CAGCTCATGG TGGTGGGACA CAGTCTCGGC 
GCAGGGGCTG CAGCACTCAT CTCCCTCCAG CTCCTGGATC ACTGCCCCGG ACTGAAGTGC 
CTGGCGTACA GCTGCCCAGG CGCGCTCGTG AGCAAGAACC TGGCACATGC CATGGCGAGC 
TTCACTACCA CGATTGTGGT GGGCAAGGAT GCCGTGCCCA GGGCCTCTGT GGCAAACCTG 
GGCAGGCTGA TGGATGAGAT GGTGACGTCC CTGGCAAGGT GCAAGCAGCC CAAGCTCAAG 
GTGACATTCT ATCCCTGGTG GAAGAGGAAG AAGCAGGACT TCAAGAATCT GTTCTGGGAC 
TATGACAAGA TCCCAGAGGA GTCACTGGAG GTGTTGAGAG GATACTATGA CAGCAGAGTC 
TCTGGAGGGC AGCCCATGAG CATGTACCCA CCTGGACGTC TCATATTCGT GCGGCCCATC 
AAGACGCGGC GCAAGAAGGC ATGGGACTGT GTCTGGATCT CTCCAGAGGA CATCATCGAC 
GAGGATGCCA AACCATCAAA GCGGGGATTC AAATGGCAAG GACGCCTGGG GAGAAGACCC 
CAGCAGCACT ACAGATATGA GAGGCAGACC AGCGAGCAGC GCAGTCTGCA CCAGGAGCTC 
ATTGCAGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011400808.1
CDS	1..3252
Translation	MPAVRLWGRRWHFSTDIVPIPAAINGAFHFVWICVLLFGAIGSGEWPSGCNSATGRQYVALFAGLFGAFVLNLIIDVLLYIHGMKGSPFEVSKRHWVPTLLHISTLPIVAQFVFTAFGTHVVNQQLDDCWPADVRHGVRVVARILVYSTWGLMAIIALGIAITYNLYPEHKSQGSWEQRCECIAVLLCCRSNMRRRPAGKRPPLSNIAELTSGLLSHVDLEATDLMTAVILTSAAQNRRRRLRLAKALLPVYEALRQTGSRSSRSDPGTAASSAGDSDSDSEAESGDEAGASSDGSSVHDVPASRRLTRRLEGGHSERGRAAAPRGEPRPEAPEVAIDTAASLPLTESNVALMDRLASASVGGEESAAGGGDDGKQEAAAVAVTNELHDLAANTGQDAGELADALADAIQDELEDLGGGDIGATHEDAVLQVDKAPSLDLALLADGASSIWLNPSVATQAPSVSNQGEETAPGSPRRTQDAEFPAGVSDSGPPDSPSVPPATGSSPSTQPPPVTVPEGVDPLPSREAVVAARQAGAPGSEGRLPSEASQGARSGSVRDLASVTLRFPTVITPSQYVNRLAGKLPPEALADIYAGRHDRVGVEVLEEATYFLKYAYAVYSLQPKIENPSSILDMACWKPPAPQDVVFSGFNELEQLEDDSTIELLHLNCSNRMLAHLPYLIALDHVKKAVVLAIRGTISAADIVTDAMVASERLEHLLPVSLKEDVKGPCFGHTGMLAAAQAIFMDMKERGILTPLVGSEAAADDYSAPRGEGPPQRESQYDADTGPSPLRAAMGSAGQAIREGVAAVLPAGGWTGAGRPGDAEGDDIEAQRAAARDSDGPRVTVDKLGAQKSPGQKVGELMKVKLLEDDWQLMVVGHSLGAGAAALISLQLLDHCPGLKCLAYSCPGALVSKNLAHAMASFTTTIVVGKDAVPRASVANLGRLMDEMVTSLARCKQPKLKVTFYPWWKRKKQDFKNLFWDYDKIPEESLEVLRGYYDSRVSGGQPMSMYPPGRLIFVRPIKTRRKKAWDCVWISPEDIIDEDAKPSKRGFKWQGRLGRRPQQHYRYERQTSEQRSLHQELIAD

Target ORF information:

RefSeq Version	XM_011402506.1
Organism	Auxenochlorella protothecoides
Definition	Auxenochlorella protothecoides Sn1-specific diacylglycerol lipase alpha partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011402506.1

ORF Insert Sequence:

ATGCCTGCCG TCCGGCTTTG GGGCCGGAGG TGGCACTTCA GCACCGACAT CGTTCCCATC 
CCCGCTGCCA TCAACGGCGC CTTCCATTTT GTCTGGATAT GCGTTCTCCT CTTCGGTGCT 
ATTGGATCAG GGGAATGGCC CAGTGGGTGC AATAGCGCGA CTGGGCGGCA GTACGTGGCG 
TTGTTCGCCG GCCTGTTTGG GGCCTTCGTT CTTAATCTCA TCATCGACGT CCTGCTCTAC 
ATCCATGGAA TGAAGGGATC TCCATTCGAG GTGTCCAAAC GGCACTGGGT TCCGACCCTC 
CTCCACATCA GCACTCTACC CATTGTCGCT CAGTTCGTGT TCACAGCATT TGGGACCCAT 
GTCGTGAATC AGCAGCTGGA CGACTGCTGG CCGGCAGACG TCAGGCATGG CGTGAGGGTG 
GTGGCGCGGA TCCTCGTCTA CTCCACCTGG GGCCTGATGG CCATCATCGC GCTGGGAATC 
GCCATCACGT ACAACCTGTA CCCGGAGCAC AAGTCGCAGG GTTCCTGGGA GCAGCGGTGC 
GAGTGCATCG CCGTGCTGCT GTGCTGCCGC AGCAACATGC GGCGCCGGCC AGCGGGCAAG 
CGCCCCCCGC TGTCCAACAT CGCGGAGCTG ACCTCCGGCC TGCTGTCGCA CGTAGACCTG 
GAGGCCACCG ACCTGATGAC GGCCGTGATC CTGACCTCCG CCGCGCAAAA CCGGCGGCGG 
CGCCTGCGCC TGGCCAAGGC GCTGCTGCCC GTGTACGAAG CGCTGCGCCA GACGGGGTCG 
CGGAGCAGCC GGTCGGATCC CGGCACGGCC GCCAGCTCCG CCGGCGACTC CGACTCGGAC 
TCCGAGGCGG AGAGCGGGGA TGAGGCGGGG GCCAGCAGCG ACGGCTCCTC GGTGCATGAC 
GTGCCAGCCA GCCGCCGTCT GACGCGGCGG CTGGAGGGCG GGCACTCGGA GCGGGGCAGG 
GCTGCGGCGC CCCGTGGGGA GCCGAGGCCA GAGGCGCCGG AGGTCGCGAT CGACACCGCC 
GCCAGCCTGC CCCTGACCGA GAGCAACGTG GCGCTGATGG ATCGGCTGGC CAGCGCCTCG 
GTGGGCGGCG AGGAAAGCGC GGCGGGGGGC GGAGACGACG GGAAGCAGGA GGCGGCGGCG 
GTGGCAGTGA CCAACGAGCT CCACGACCTG GCTGCCAACA CGGGGCAAGA CGCGGGGGAA 
TTGGCCGATG CACTGGCGGA TGCCATCCAG GATGAGCTGG AGGATCTGGG TGGCGGGGAC 
ATCGGGGCCA CCCACGAGGA CGCGGTTCTC CAAGTGGACA AGGCCCCCTC GCTGGATCTG 
GCCCTGCTGG CAGACGGCGC CTCCTCCATC TGGCTGAACC CCAGCGTCGC AACGCAGGCC 
CCCTCCGTCT CCAATCAGGG CGAGGAGACG GCACCCGGCT CTCCCCGCCG GACCCAGGAC 
GCCGAGTTCC CGGCCGGAGT CTCTGACTCC GGGCCCCCAG ACTCGCCATC AGTGCCCCCC 
GCGACGGGAA GCAGCCCCTC CACCCAGCCC CCGCCGGTGA CGGTGCCGGA GGGCGTGGAC 
CCGCTGCCCT CCCGCGAGGC CGTGGTGGCG GCCAGGCAGG CCGGCGCGCC GGGGTCGGAG 
GGCCGCCTGC CCTCCGAGGC CAGCCAGGGG GCGCGCAGCG GCAGCGTGCG CGACCTGGCC 
AGCGTTACGC TGCGCTTCCC CACCGTCATC ACGCCTTCCC AGTACGTCAA CCGCCTGGCA 
GGGAAGCTGC CGCCCGAGGC GCTGGCCGAC ATCTACGCTG GCCGGCATGA CCGCGTGGGG 
GTGGAGGTCC TGGAGGAGGC CACCTACTTC CTCAAGTACG CCTACGCTGT CTACTCCCTG 
CAGCCCAAGA TCGAGAACCC CTCCAGCATC CTGGACATGG CCTGCTGGAA GCCGCCCGCG 
CCCCAGGACG TGGTTTTTTC AGGGTTCAAT GAGCTGGAGC AACTGGAGGA TGACTCCACC 
ATCGAGCTGC TGCACCTCAA CTGCTCCAAC CGGATGCTGG CGCACCTGCC CTACCTCATC 
GCTCTGGACC ATGTGAAGAA GGCGGTGGTG CTGGCCATCA GGGGTACCAT CTCTGCCGCC 
GACATTGTGA CGGATGCCAT GGTCGCCAGC GAGCGCCTGG AGCACCTGCT CCCTGTCAGC 
CTGAAGGAGG ACGTCAAGGG CCCCTGCTTC GGGCACACGG GCATGCTGGC GGCGGCCCAG 
GCCATCTTCA TGGACATGAA GGAGCGGGGC ATCCTGACCC CGCTAGTGGG CAGCGAGGCG 
GCGGCTGACG ACTACTCCGC GCCGCGCGGG GAGGGCCCGC CCCAGCGGGA ATCCCAGTAC 
GACGCCGACA CGGGGCCCAG CCCCCTGCGC GCGGCCATGG GCAGCGCGGG GCAGGCCATT 
CGCGAGGGCG TCGCGGCGGT GCTGCCCGCG GGGGGCTGGA CAGGGGCGGG CAGGCCAGGG 
GATGCCGAAG GGGACGACAT AGAGGCACAG CGCGCGGCGG CGAGAGATTC CGACGGACCA 
CGTGTCACGG TGGACAAGCT GGGGGCGCAA AAGTCGCCGG GCCAGAAGGT GGGGGAGCTG 
ATGAAGGTGA AGCTGCTGGA GGACGACTGG CAGCTCATGG TGGTGGGACA CAGTCTCGGC 
GCAGGGGCTG CAGCACTCAT CTCCCTCCAG CTCCTGGATC ACTGCCCCGG ACTGAAGTGC 
CTGGCGTACA GCTGCCCAGG CGCGCTCGTG AGCAAGAACC TGGCACATGC CATGGCGAGC 
TTCACTACCA CGATTGTGGT GGGCAAGGAT GCCGTGCCCA GGGCCTCTGT GGCAAACCTG 
GGCAGGCTGA TGGATGAGAT GGTGACGTCC CTGGCAAGGT GCAAGCAGCC CAAGCTCAAG 
GTGACATTCT ATCCCTGGTG GAAGAGGAAG AAGCAGGACT TCAAGAATCT GTTCTGGGAC 
TATGACAAGA TCCCAGAGGA GTCACTGGAG GTGTTGAGAG GATACTATGA CAGCAGAGTC 
TCTGGAGGGC AGCCCATGAG CATGTACCCA CCTGGACGTC TCATATTCGT GCGGCCCATC 
AAGACGCGGC GCAAGAAGGC ATGGGACTGT GTCTGGATCT CTCCAGAGGA CATCATCGAC 
GAGGATGCCA AACCATCAAA GCGGGGATTC AAATGGCAAG GACGCCTGGG GAGAAGACCC 
CAGCAGCACT ACAGATATGA GAGGCAGACC AGCGAGCAGC GCAGTCTGCA CCAGGAGCTC 
ATTGCAGATT GA