F751_3671 cDNA ORF clone, Auxenochlorella protothecoides

Gene
Clones
gRNAs

Summary

Gene Symbol	F751_3671
Entrez Gene ID	23615062
Full Name	Pre-mRNA-splicing factor CWC22
Gene Type	protein-coding
Organism	Auxenochlorella protothecoides

mRNA and Protein(s)

mRNA	Protein	Name
XM_011400627.1	XP_011398929.1	Pre-mRNA-splicing factor CWC22

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Oil accumulation mechanisms of the oleaginous microalga Chlorella protothecoides revealed through its genome, transcriptomes, and proteomes.
Gao C, Wang Y, Shen Y, Yan D, He X, Dai J, Wu Q
BMC genomics15582(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following F751_3671 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F751_3671 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAu62053	XM_011400627.1 Latest version!	Auxenochlorella protothecoides Pre-mRNA-splicing factor CWC22 partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F751_3671 ( XM_011400627.1 ) cDNA ORF clone, Auxenochlorella protothecoides -> XP_011398929.1 Auxenochlorella protothecoides Pre-mRNA-splicing factor CWC22 partial mRNA.

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CloneID	OAu62053
Clone ID Related Accession (Same CDS sequence)	XM_011400627.1
Accession Version	XM_011400627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-23
Organism	Auxenochlorella protothecoides
Product	Pre-mRNA-splicing factor CWC22
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_011934253). COMPLETENESS: incomplete on both ends.

ATGCTGGCGG AGGCGCAGGA CACCACCAGC GTGCAGTACC AGCGCATGAC CTGGGACGCG 
CTGCGCAAGT CCCTCAACGG GCTGATCAAC AAGGTGAACG CCGCCAACAT CAAGCACATC 
CTGCCAGAGT TCTTCTCCGA GAATCTGGTG CGTGGTCGGG GCCTGTTCTG CCGCAGCCTG 
ATGAAGAGCC AGATGGCGTC CCCCTCCTTC ACCCCCGTCT ACGCCGCGCT GGTGGCGGTG 
TTCAACACCA AGTTCCCGGA GCTGGGCGAG CTGCTGCTGC ACCGTGTGGT CACCCAGTTC 
CGCCGCGCCT ACAAGCGTAA CGACAAGCCT GTCTGCCTGG CGGCCATCAA GTTCCTGGCC 
CACCTGGCCA ACCAGCAGGT GGCGCACGAG GTGCTGGCCC TGGAGGTGCT CATGCTCCTG 
CTGGAGACGC CCTCCGACGA CGGCGTGGAG GTGGCCGCCG GGTTTGTCAC CGAGGTGGGG 
GCCCTGCTCC AGGACCTGGC GCCCCAGGGG CTCAATTCCG TCTTTGACCG ATTCAGGGCC 
ATCCTGCATG AGGGCAGCAT TGACAAGAGG ACTCAGTTCA TCATAGAAAA CCTGTTTGCC 
GTGCGCAAGG CCGGCTTCGA GCAGCAGGGG CACCCCGCGA TTCCCGCGGA CCTGGATCTG 
GTGGAGACAG AGGATCAGAT CACTCACGAG GTGTCGCTGG ACGACAAGCT GGACACCCAG 
ATGATGCTGG ACGTGTTCCA GGAGGACCCC GACTTTGCCC AGCATGAGGC CGAGTACCAG 
GCGATCAGGC GTGAGATCCT GGGTGAGGAA AGCGGGGACG AGGAGGAGGA GGAGTCGGGG 
AGCTCCTCTG GCGAGGAGGA GGAGGACGGG GAGGAGGGCG GGCACGCCGC TGGGCCGTCG 
GGCGTCGGGG GCCCCATCTC CGACCAGACG CAGACGGACC TGATCAACCT GCGGCGAACC 
ATCTACCTCA CCATCATGTC TGCCCTGGAC TTTGAAGAGG CGGGACACAA GCTCCTGAAG 
ATGGGCATAC CTGCCGGCCT GGAGATGGAG CTGTGCACCA TGATCATCGA GTGCTGCTCC 
CAGGAGAAGA CCTTCATAAA GTACTACGCG CTGCTGGGGG AGCGCTTCTC CAAGCTCAAG 
CGCGAGTACT GCGACTGCTT CTCCGAGTGC TTCGTGCGCC AGTACTCGCT CATCCACCGC 
CTGGAGACCA ACAAGCTGCG CAACGTGGCC AAGCTCTTCG CCCACCTGCT GGCCAGCGAC 
GCCATCCCCT GGTCCGTGCT GGAGGTGGTG CGGCTGACGG AGGAGGACAC CACCTCCTCC 
TCCCGCATCT TTGTCAAGAT CCTGTTCCAG GAGCTGGTGG AGCAGCTGGG CCTGCTGGGC 
CTCAACAAGA GGCTGCAGGA CCCAAGCTGT GCCGCGTGGT ACACCGGCAT CTTCCCCACC 
GACAGCCCGC GCAACATGCG CTTCTCCATC AACTTCTTCA CCTCCATCGG GCTGGGAGGG 
CTGACGGATC GCATGCGCGA GACGCTGAAG AACCTGCCGG CGCTCATCGC GGCGCAGCGG 
GCTGCAGCAG AGGCGGCCAA GAACGACGCC TCCTCGTCCT CGGACGATTC GTCATCTGCA 
TCATCTGGCG GCTCATCATC CTCAGACGAC TCCTCGTCGT CAGGCGGCTC CTCTTCCAGC 
GACTCCTCGT CGTCCTCCTC CTCCTCATCA TCGTCATCTG GCTCCGACAG CAGCAGCAGC 
GGGTCGGAGT CGGAGGCGGA GGCGGCCAGG CGCGCGCGGC GGAAGCGGGG TGATGACGGC 
AATGGCAGGG CGGACCCCTC CAAGAAGTCC CGCAGGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011398929.1
CDS	1..1842
Translation	MLAEAQDTTSVQYQRMTWDALRKSLNGLINKVNAANIKHILPEFFSENLVRGRGLFCRSLMKSQMASPSFTPVYAALVAVFNTKFPELGELLLHRVVTQFRRAYKRNDKPVCLAAIKFLAHLANQQVAHEVLALEVLMLLLETPSDDGVEVAAGFVTEVGALLQDLAPQGLNSVFDRFRAILHEGSIDKRTQFIIENLFAVRKAGFEQQGHPAIPADLDLVETEDQITHEVSLDDKLDTQMMLDVFQEDPDFAQHEAEYQAIRREILGEESGDEEEEESGSSSGEEEEDGEEGGHAAGPSGVGGPISDQTQTDLINLRRTIYLTIMSALDFEEAGHKLLKMGIPAGLEMELCTMIIECCSQEKTFIKYYALLGERFSKLKREYCDCFSECFVRQYSLIHRLETNKLRNVAKLFAHLLASDAIPWSVLEVVRLTEEDTTSSSRIFVKILFQELVEQLGLLGLNKRLQDPSCAAWYTGIFPTDSPRNMRFSINFFTSIGLGGLTDRMRETLKNLPALIAAQRAAAEAAKNDASSSSDDSSSASSGGSSSSDDSSSSGGSSSSDSSSSSSSSSSSSGSDSSSSGSESEAEAARRARRKRGDDGNGRADPSKKSRRR

Target ORF information:

RefSeq Version	XM_011400627.1
Organism	Auxenochlorella protothecoides
Definition	Auxenochlorella protothecoides Pre-mRNA-splicing factor CWC22 partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011400627.1

ORF Insert Sequence:

ATGCTGGCGG AGGCGCAGGA CACCACCAGC GTGCAGTACC AGCGCATGAC CTGGGACGCG 
CTGCGCAAGT CCCTCAACGG GCTGATCAAC AAGGTGAACG CCGCCAACAT CAAGCACATC 
CTGCCAGAGT TCTTCTCCGA GAATCTGGTG CGTGGTCGGG GCCTGTTCTG CCGCAGCCTG 
ATGAAGAGCC AGATGGCGTC CCCCTCCTTC ACCCCCGTCT ACGCCGCGCT GGTGGCGGTG 
TTCAACACCA AGTTCCCGGA GCTGGGCGAG CTGCTGCTGC ACCGTGTGGT CACCCAGTTC 
CGCCGCGCCT ACAAGCGTAA CGACAAGCCT GTCTGCCTGG CGGCCATCAA GTTCCTGGCC 
CACCTGGCCA ACCAGCAGGT GGCGCACGAG GTGCTGGCCC TGGAGGTGCT CATGCTCCTG 
CTGGAGACGC CCTCCGACGA CGGCGTGGAG GTGGCCGCCG GGTTTGTCAC CGAGGTGGGG 
GCCCTGCTCC AGGACCTGGC GCCCCAGGGG CTCAATTCCG TCTTTGACCG ATTCAGGGCC 
ATCCTGCATG AGGGCAGCAT TGACAAGAGG ACTCAGTTCA TCATAGAAAA CCTGTTTGCC 
GTGCGCAAGG CCGGCTTCGA GCAGCAGGGG CACCCCGCGA TTCCCGCGGA CCTGGATCTG 
GTGGAGACAG AGGATCAGAT CACTCACGAG GTGTCGCTGG ACGACAAGCT GGACACCCAG 
ATGATGCTGG ACGTGTTCCA GGAGGACCCC GACTTTGCCC AGCATGAGGC CGAGTACCAG 
GCGATCAGGC GTGAGATCCT GGGTGAGGAA AGCGGGGACG AGGAGGAGGA GGAGTCGGGG 
AGCTCCTCTG GCGAGGAGGA GGAGGACGGG GAGGAGGGCG GGCACGCCGC TGGGCCGTCG 
GGCGTCGGGG GCCCCATCTC CGACCAGACG CAGACGGACC TGATCAACCT GCGGCGAACC 
ATCTACCTCA CCATCATGTC TGCCCTGGAC TTTGAAGAGG CGGGACACAA GCTCCTGAAG 
ATGGGCATAC CTGCCGGCCT GGAGATGGAG CTGTGCACCA TGATCATCGA GTGCTGCTCC 
CAGGAGAAGA CCTTCATAAA GTACTACGCG CTGCTGGGGG AGCGCTTCTC CAAGCTCAAG 
CGCGAGTACT GCGACTGCTT CTCCGAGTGC TTCGTGCGCC AGTACTCGCT CATCCACCGC 
CTGGAGACCA ACAAGCTGCG CAACGTGGCC AAGCTCTTCG CCCACCTGCT GGCCAGCGAC 
GCCATCCCCT GGTCCGTGCT GGAGGTGGTG CGGCTGACGG AGGAGGACAC CACCTCCTCC 
TCCCGCATCT TTGTCAAGAT CCTGTTCCAG GAGCTGGTGG AGCAGCTGGG CCTGCTGGGC 
CTCAACAAGA GGCTGCAGGA CCCAAGCTGT GCCGCGTGGT ACACCGGCAT CTTCCCCACC 
GACAGCCCGC GCAACATGCG CTTCTCCATC AACTTCTTCA CCTCCATCGG GCTGGGAGGG 
CTGACGGATC GCATGCGCGA GACGCTGAAG AACCTGCCGG CGCTCATCGC GGCGCAGCGG 
GCTGCAGCAG AGGCGGCCAA GAACGACGCC TCCTCGTCCT CGGACGATTC GTCATCTGCA 
TCATCTGGCG GCTCATCATC CTCAGACGAC TCCTCGTCGT CAGGCGGCTC CTCTTCCAGC 
GACTCCTCGT CGTCCTCCTC CTCCTCATCA TCGTCATCTG GCTCCGACAG CAGCAGCAGC 
GGGTCGGAGT CGGAGGCGGA GGCGGCCAGG CGCGCGCGGC GGAAGCGGGG TGATGACGGC 
AATGGCAGGG CGGACCCCTC CAAGAAGTCC CGCAGGCGCT GA