F751_2084 cDNA ORF clone, Auxenochlorella protothecoides

Gene
Clones
gRNAs

Summary

Gene Symbol	F751_2084
Entrez Gene ID	23613475
Full Name	Phospholipase A(1) DAD1, chloroplastic
Gene Type	protein-coding
Organism	Auxenochlorella protothecoides

mRNA and Protein(s)

mRNA	Protein	Name
XM_011401133.1	XP_011399435.1	Phospholipase A(1) DAD1, chloroplastic

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Oil accumulation mechanisms of the oleaginous microalga Chlorella protothecoides revealed through its genome, transcriptomes, and proteomes.
Gao C, Wang Y, Shen Y, Yan D, He X, Dai J, Wu Q
BMC genomics15582(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following F751_2084 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F751_2084 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAu62553	XM_011401133.1 Latest version!	Auxenochlorella protothecoides Phospholipase A(1) DAD1, chloroplastic partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F751_2084 ( XM_011401133.1 ) cDNA ORF clone, Auxenochlorella protothecoides -> XP_011399435.1 Auxenochlorella protothecoides Phospholipase A(1) DAD1, chloroplastic partial mRNA.

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CloneID	OAu62553
Clone ID Related Accession (Same CDS sequence)	XM_011401133.1
Accession Version	XM_011401133.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-23
Organism	Auxenochlorella protothecoides
Product	Phospholipase A(1) DAD1, chloroplastic
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_011934268). COMPLETENESS: incomplete on both ends.

ATGCGCGTCC GCCAGACTGA GATGCTCCGA CCGCTGCGTG CTTTGCACGC TGGTCAGCGT 
GCATGTCAGG ACCTGTCTCG AGACTTTGGG AAGAGAGACT TCCAGAGCGC CCCTGCAGCC 
GCCGGGAATG ACTTTGACTG GCGACAGTCC GTCGTGTTGG CAGGGGCGTC CTTTGAGGCT 
TACTTTGAGC TGGCGCACAA GACCAACGCC GTGCACATCG ATACCAGCGA CGCGTGCATC 
ACCCACGTCG ACGAGACGTT CCTGAGGCAG TGCATGGATG GCCTGCTACA TGTGGAGCTC 
CTGAGGGCGA CTGGGCTGCC CAGCGAAGAG TTGTGGGACG TGAGCAACCG GGTCTACTGC 
ACCGTCGAGC TCGGGGGCAG CAAGGTGGAG ACGCGCGCCT TGGAGAAGCC AGCAAACGGC 
GGGAAGGGGA TGAAGGCCTT GCTGCCCGGT CCCAAGGAGG AGAAGCCATC GCCCCCTCCC 
GCCCCCAAGC TGCTCTTCAT CCAGGACCGC GCCAAGGCGC GCCGGCTGCG TGTGAAGCTG 
TGGAGGAGCC GGCTGGGGCG GCCACTGGAG GCGCTGGGGA GCGGGGAGCT GGACCTCCAG 
CAGGGCCTGG GAGGACGGGG GGGCTGGCGC GAGGTGGCGA TCACCCTGAA GCCCGACGCG 
AAGGAGGCTG CTGGGGATGA GTCATCGGCG GCTGCGCTCC CCAGCTCTGA GGCCGAGGAA 
GGGAAGGCGG AGGCAGAGCA GCCCCGTGTG GTCACCCTCA GCCTTCAGTA TGTGGCCATG 
GAAGTCTGCG TGGGGGCCCC CGGGGAGGAG TTTGAGAGCA ACCCCTGGCG CGAGCTGCAG 
GAGGCCGCTG CCATCCAGCT GGGAGAGACC TTCACCCCGC TCTGCTTCAT CACCCACCCC 
CGCACGGACA CCCAGGCCTG GCTGTACTGG AACCTGGGCA AGCGCCAGCT GTGCATCGCC 
TTCAGGGGCA CCGAGCAGGA CAACTGGCGG GACTACCTCA CTGACATCTC CCTCGCCCCG 
GCCCTGCTGG ACCCCGCGGC CGTGCCGACG CTGCCGCTGC ATCGCGGTAG GGCGTGGGGT 
GGTACTTTGT TGGCTGAGCG CTCAGGCCGC TGTGGAAGGA TGGTGTGCGG TCAAGGCGTA 
CATGCGCGGC GGTTCCAGGA CCTCTCCCTG CGGCATCACN NNNNNNNNNN NNNNNNNNNN 
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NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC CCAGCGTGGT GCCCGCCGCG 
CTGGAGGCGG TCAAGGGCAG CGCCGGCGAG GCCCGGGTCC TGCTGGACCG CCTCTGGCAG 
GCCGTGTCGG AGGCCGAGGC GGTGGCCGAG GAGGCCGGGG CCGAGGACCG GCCCTGGGTG 
CACCAGGGCT TCCTGGCTGC CTACGACAGC GTGCGCGGCG CGGCGCTGGG GCTGGTGCGC 
GCGGCGCTGG CGCGCGGCGG CGGCGGCGGC GAGCCGTGGC ACGTGTTTGT CACAGGGCAC 
TCGCTGGGGG GCGCGCTGGG CACGCTGTGC TCCTATGACC TGGCGCTGCA CGACTGGGAC 
GGGGGGCCGG CGCCCCACCT CACCATGTAC AACTTTGGCA GCCCGCGCGT CGGCAACCGC 
CGCTTCGCAC GGGAGTACAA CGACCTCGTC CCCAACAGCT GGCGCATCGT CAACAACCAG 
GACGCTGTGT GCTCGGTCCC TCGCCTCATG GGGTACTGCC ACGTGGGCCA CGCCGTGCGC 
CTGCTGGGCG ACGGCCGGGT GGAGGTGCAG CGGGACACCA GCCTGGCGCT GGGAGAGGGC 
ACGGCCATGC CCGACATCAT GCCCGCCGTC GCTGGCATGA TCACGGCCGT AGTGCCTCAG 
TTCACCTCCA AGGCAACGGC GATGGTGATG GCGGCCATTC CCGCGCTGGC CAAGCGTGCG 
GCGGATCGCG CCTCGGCGGC CGTGGCCGCG ACGACGGACC CGGACGATGT CTCGGCCACC 
CGCGACGAGC TGGAAGCCGC GCAGAAGCTG TGGGGCGAGG AGGTGGCGGC ATGGTCGGCC 
ATGCTGGACC GCGCCTCCTT CTCCCAGCAC CTGGAGGAGC TGTATCTGGA GAGCCTGAGC 
TCCTGCGTGC GCCGCACCTG GGCGCCTGTC GACGGCGGGG ACTCCGCAGC TGACGTGGAG 
GAGAAGGCAC CAGAACAAGC CGACCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011399435.1
CDS	1..3210
Translation	MRVRQTEMLRPLRALHAGQRACQDLSRDFGKRDFQSAPAAAGNDFDWRQSVVLAGASFEAYFELAHKTNAVHIDTSDACITHVDETFLRQCMDGLLHVELLRATGLPSEELWDVSNRVYCTVELGGSKVETRALEKPANGGKGMKALLPGPKEEKPSPPPAPKLLFIQDRAKARRLRVKLWRSRLGRPLEALGSGELDLQQGLGGRGGWREVAITLKPDAKEAAGDESSAAALPSSEAEEGKAEAEQPRVVTLSLQYVAMEVCVGAPGEEFESNPWRELQEAAAIQLGETFTPLCFITHPRTDTQAWLYWNLGKRQLCIAFRGTEQDNWRDYLTDISLAPALLDPAAVPTLPLHRGRAWGGTLLAERSGRCGRMVCGQGVHARRFQDLSLRHHnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullPSVVPAALEAVKGSAGEARVLLDRLWQAVSEAEAVAEEAGAEDRPWVHQGFLAAYDSVRGAALGLVRAALARGGGGGEPWHVFVTGHSLGGALGTLCSYDLALHDWDGGPAPHLTMYNFGSPRVGNRRFAREYNDLVPNSWRIVNNQDAVCSVPRLMGYCHVGHAVRLLGDGRVEVQRDTSLALGEGTAMPDIMPAVAGMITAVVPQFTSKATAMVMAAIPALAKRAADRASAAVAATTDPDDVSATRDELEAAQKLWGEEVAAWSAMLDRASFSQHLEELYLESLSSCVRRTWAPVDGGDSAADVEEKAPEQADP

Target ORF information:

RefSeq Version	XM_011401133.1
Organism	Auxenochlorella protothecoides
Definition	Auxenochlorella protothecoides Phospholipase A(1) DAD1, chloroplastic partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011401133.1

ORF Insert Sequence:

ATGCGCGTCC GCCAGACTGA GATGCTCCGA CCGCTGCGTG CTTTGCACGC TGGTCAGCGT 
GCATGTCAGG ACCTGTCTCG AGACTTTGGG AAGAGAGACT TCCAGAGCGC CCCTGCAGCC 
GCCGGGAATG ACTTTGACTG GCGACAGTCC GTCGTGTTGG CAGGGGCGTC CTTTGAGGCT 
TACTTTGAGC TGGCGCACAA GACCAACGCC GTGCACATCG ATACCAGCGA CGCGTGCATC 
ACCCACGTCG ACGAGACGTT CCTGAGGCAG TGCATGGATG GCCTGCTACA TGTGGAGCTC 
CTGAGGGCGA CTGGGCTGCC CAGCGAAGAG TTGTGGGACG TGAGCAACCG GGTCTACTGC 
ACCGTCGAGC TCGGGGGCAG CAAGGTGGAG ACGCGCGCCT TGGAGAAGCC AGCAAACGGC 
GGGAAGGGGA TGAAGGCCTT GCTGCCCGGT CCCAAGGAGG AGAAGCCATC GCCCCCTCCC 
GCCCCCAAGC TGCTCTTCAT CCAGGACCGC GCCAAGGCGC GCCGGCTGCG TGTGAAGCTG 
TGGAGGAGCC GGCTGGGGCG GCCACTGGAG GCGCTGGGGA GCGGGGAGCT GGACCTCCAG 
CAGGGCCTGG GAGGACGGGG GGGCTGGCGC GAGGTGGCGA TCACCCTGAA GCCCGACGCG 
AAGGAGGCTG CTGGGGATGA GTCATCGGCG GCTGCGCTCC CCAGCTCTGA GGCCGAGGAA 
GGGAAGGCGG AGGCAGAGCA GCCCCGTGTG GTCACCCTCA GCCTTCAGTA TGTGGCCATG 
GAAGTCTGCG TGGGGGCCCC CGGGGAGGAG TTTGAGAGCA ACCCCTGGCG CGAGCTGCAG 
GAGGCCGCTG CCATCCAGCT GGGAGAGACC TTCACCCCGC TCTGCTTCAT CACCCACCCC 
CGCACGGACA CCCAGGCCTG GCTGTACTGG AACCTGGGCA AGCGCCAGCT GTGCATCGCC 
TTCAGGGGCA CCGAGCAGGA CAACTGGCGG GACTACCTCA CTGACATCTC CCTCGCCCCG 
GCCCTGCTGG ACCCCGCGGC CGTGCCGACG CTGCCGCTGC ATCGCGGTAG GGCGTGGGGT 
GGTACTTTGT TGGCTGAGCG CTCAGGCCGC TGTGGAAGGA TGGTGTGCGG TCAAGGCGTA 
CATGCGCGGC GGTTCCAGGA CCTCTCCCTG CGGCATCACN NNNNNNNNNN NNNNNNNNNN 
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NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC CCAGCGTGGT GCCCGCCGCG 
CTGGAGGCGG TCAAGGGCAG CGCCGGCGAG GCCCGGGTCC TGCTGGACCG CCTCTGGCAG 
GCCGTGTCGG AGGCCGAGGC GGTGGCCGAG GAGGCCGGGG CCGAGGACCG GCCCTGGGTG 
CACCAGGGCT TCCTGGCTGC CTACGACAGC GTGCGCGGCG CGGCGCTGGG GCTGGTGCGC 
GCGGCGCTGG CGCGCGGCGG CGGCGGCGGC GAGCCGTGGC ACGTGTTTGT CACAGGGCAC 
TCGCTGGGGG GCGCGCTGGG CACGCTGTGC TCCTATGACC TGGCGCTGCA CGACTGGGAC 
GGGGGGCCGG CGCCCCACCT CACCATGTAC AACTTTGGCA GCCCGCGCGT CGGCAACCGC 
CGCTTCGCAC GGGAGTACAA CGACCTCGTC CCCAACAGCT GGCGCATCGT CAACAACCAG 
GACGCTGTGT GCTCGGTCCC TCGCCTCATG GGGTACTGCC ACGTGGGCCA CGCCGTGCGC 
CTGCTGGGCG ACGGCCGGGT GGAGGTGCAG CGGGACACCA GCCTGGCGCT GGGAGAGGGC 
ACGGCCATGC CCGACATCAT GCCCGCCGTC GCTGGCATGA TCACGGCCGT AGTGCCTCAG 
TTCACCTCCA AGGCAACGGC GATGGTGATG GCGGCCATTC CCGCGCTGGC CAAGCGTGCG 
GCGGATCGCG CCTCGGCGGC CGTGGCCGCG ACGACGGACC CGGACGATGT CTCGGCCACC 
CGCGACGAGC TGGAAGCCGC GCAGAAGCTG TGGGGCGAGG AGGTGGCGGC ATGGTCGGCC 
ATGCTGGACC GCGCCTCCTT CTCCCAGCAC CTGGAGGAGC TGTATCTGGA GAGCCTGAGC 
TCCTGCGTGC GCCGCACCTG GGCGCCTGTC GACGGCGGGG ACTCCGCAGC TGACGTGGAG 
GAGAAGGCAC CAGAACAAGC CGACCCGTGA