LOC105688847 cDNA ORF clone, Athalia rosae(coleseed sawfly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105688847
Entrez Gene ID	105688847
Full Name	DNA polymerase eta
Gene Type	protein-coding
Organism	Athalia rosae(coleseed sawfly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012405443.2	XP_012260866.1	DNA polymerase eta isoform X2
XM_012405442.2	XP_012260865.1	DNA polymerase eta isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC105688847 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105688847 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAt104356	XM_012405442.2 Latest version!	Athalia rosae DNA polymerase eta (LOC105688847), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAt104357	XM_012405443.2 Latest version!	Athalia rosae DNA polymerase eta (LOC105688847), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105688847 ( XM_012405442.2 ) cDNA ORF clone, Athalia rosae(coleseed sawfly) -> XP_012260865.1 Athalia rosae DNA polymerase eta (LOC105688847), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAt104356
Clone ID Related Accession (Same CDS sequence)	XM_012405442.2
Accession Version	XM_012405442.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-16
Organism	Athalia rosae(coleseed sawfly)
Product	DNA polymerase eta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020311971.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 20, 2017 this sequence version replaced XM_012405442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Athalia rosae Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTATG GCGGGAAAAT ATCATCACGT GTAGATTCGC GGAGAAGAAA CTGTTCGCCC 
GCTCAAAAGT CTCTACTCTT CGGCCACATG GACATGGATT GCTTTTTTTG CCAAGTCGAA 
GTGAAGTTAC AACCAGAACT ACAGGGAAAA CCATTAGCTG TTGTACAGTT CAATCAGTGG 
CAATCTGGAG GGATTATTGC TGTTAACTAT GAGGCACGAG ATTGTGGAGT TAGCCGACAC 
ATGCGGCCTG AAGAGGCTAA AAAGAAGTGT CCAGAACTGG TCTTGGCAAC TGTTCCCGGG 
GTTCGCGGCA AAGCTGATAC ATCAAGGTAC AGAATTGCCG GCCGTGAAGT GGTTAATGTA 
CTAAAAAAGC ACAGTAACAT CATAGAACGT GCAAGTGTCG ATGAGGCATA CTTAGATATA 
ACAGAACTTG TTAATCAACA AATGATTTCC AGTCAATCAT TGTCATCAGA AATGATAGAT 
CATCTTCCAA ACACGTATGT CATTGGATAT TCAACAGTGG GAAACACTGA CGAAGCCCAA 
AGACGTAAAG CTGTGGAAAA ATGGATATCC GAAGCTTATG GAGAATTGGG AGACATCCAA 
TCTAGGAGGC TTGCTATAGC TGGTACCATA GTTGAGTCTA TAAGAGCTGA TGTGTATAAA 
ACAACGGGAT ACAGATGTTC TGCCGGGGTT TCGTATAACA AGATCTTGGC AAAGCTTGCT 
TGTGGCCTTC ATAAACCCAA CAGACAGACC ATTTTACCTC TGAGTGGGGT TCCATCCCTT 
TATCAAACAT TGCCTGTGAA GAAGGTGCGA AATCTTGGTG GTAAATTTGG AGATTTGGTC 
ATTGAGTCCT TGGGATGTAA TGTTATGGCA GATTTGTTAT CGTATTCATT AAAGGATCTC 
CAAGTACGTT TTGATGATAA AACAGGTTTG TGGTTGTATA ACATTGCTCG TGGTGTAGAT 
AATGAGCCAG TTACCAACAG GCTCATATCC AAATCGATAG GGAGTTGTAA AAATTTTCCT 
GGAAAGCGGG CTATTGTAAC AAAAGAAACT CTTGAACATT GGGTTATGCA GTTGTCTTTA 
GAAATGAGCG AACGACTAGA TCAAGATATG GAGGAGAACA ATCGCAAACC AACCTTGCTG 
ACAGTGAGTT ACCACTACTA TAAGGACAAT AAAATTATAA ATCAATCCCG ATCTTGCCGA 
TTAACTTCAA ACAAAGCTGA AATTATCATG AAAAACTGTT TGGATATCAT AATTAAATTT 
ACCAAATATC CAATCGTGTT TATGGGAATG TCTGCAAGTA AGTTCATTGA AATTGACAAA 
GAGCAAGCAA ATTTCAGGAA TTTCTTCAAA GCAAATATAG ATGTAAGCAA TAAATTGGTA 
CCCAATTGTA CACTCTTGGC AGAGGATACA GGTGACACTT CTTCATTGGA TACTGAATGC 
CCAAGTAATG AAACTTCATG TAATAATAAA ATGAGTTCTG GTGAATCTGA TATCGATGAA 
GCGCGGAGCA GACCTGTGGG CTCGGAGTGT TCTAAATTTT TTGCTGCAGC TCAACAGGAC 
GATAAAAGTA TCGCATCTAG TAGTCACGGC ATGAAAACTG AAAATAAGGA GGATACTTCT 
AAAATCATAT CTCCAGTATC ATTGCTGAGA GCTGAGAATA AAAAATTAAA ACCAGACAAC 
TTTAAGAAAT CATTTTTCAT GAATATTCTA ACTTCTAATA GAATTTCGTC AGAGCACGAA 
TCACCGGGTG GATCTGGTTT TGATGTTACA AGTTGCAGTG GGGAGCTATC TGCTGAAAAT 
GCAAAGCCTG AAAAAAATCC AAATGAAACC TCTGGTGATG TTCTACCACA AGAACTTCAA 
GATTCGGAAA ACTTTAAAAA TCAAAAGGAT GTGAAGAAAA AGGATTCTAG TTCAATTAAT 
ACCATAGAAC CAGAGGAACT TTTTCGCTCT ACCAATTGTA CAAGTCAAAC AGAAGAATCC 
GAATTTTTAC TGAGTAATAG AACTGATGTT GATGACTGTG ACGACAACTC TATAAAACTG 
CAAGATATAT TTCCAGATTT AAATAACCTT GATGAGAGCG TTGTAGCAGT TCTACCTCAG 
AGGCTACAAC GAGAAGCTAA GGCAATTTTG CAAAGTCGAA GTAAAGGCAA TCTAGAAAGT 
AAACTGAATA CAGTGAAAGT AAAGGAGCAG AAAAGTAAAG AGCAGAAGCG ATCACGCGGA 
AAAGTGACAG GTCAAACACG TCTGAATAAA ATAGATTCAT TTTTTAGCAA TCAAATTAAA 
CCGTTAGGAA GTTCTAACGA TGCAACCTTG ACAGAAAAAT GTTCCGAGTG CGATAAATTA 
ATCCCAGTTA GTGAAATCCT GGAACATGCA GATTTTCACA TAGCACGAAA ATTATCCAAA 
GAGATTAATA AATCTTTGAT CAGCTCCAAG GTGATGACTC CAGAAAAAGC GACCACAAAT 
GGTAAGCCAT CAAAGAGAAA ACGTAATAAG CCTGAAAGTC GAGAACCAGA AAAACGATCT 
AAGGGCATAG CAGCATTTTT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012260865.1
CDS	22..2568
Translation	MRYGGKISSRVDSRRRNCSPAQKSLLFGHMDMDCFFCQVEVKLQPELQGKPLAVVQFNQWQSGGIIAVNYEARDCGVSRHMRPEEAKKKCPELVLATVPGVRGKADTSRYRIAGREVVNVLKKHSNIIERASVDEAYLDITELVNQQMISSQSLSSEMIDHLPNTYVIGYSTVGNTDEAQRRKAVEKWISEAYGELGDIQSRRLAIAGTIVESIRADVYKTTGYRCSAGVSYNKILAKLACGLHKPNRQTILPLSGVPSLYQTLPVKKVRNLGGKFGDLVIESLGCNVMADLLSYSLKDLQVRFDDKTGLWLYNIARGVDNEPVTNRLISKSIGSCKNFPGKRAIVTKETLEHWVMQLSLEMSERLDQDMEENNRKPTLLTVSYHYYKDNKIINQSRSCRLTSNKAEIIMKNCLDIIIKFTKYPIVFMGMSASKFIEIDKEQANFRNFFKANIDVSNKLVPNCTLLAEDTGDTSSLDTECPSNETSCNNKMSSGESDIDEARSRPVGSECSKFFAAAQQDDKSIASSSHGMKTENKEDTSKIISPVSLLRAENKKLKPDNFKKSFFMNILTSNRISSEHESPGGSGFDVTSCSGELSAENAKPEKNPNETSGDVLPQELQDSENFKNQKDVKKKDSSSINTIEPEELFRSTNCTSQTEESEFLLSNRTDVDDCDDNSIKLQDIFPDLNNLDESVVAVLPQRLQREAKAILQSRSKGNLESKLNTVKVKEQKSKEQKRSRGKVTGQTRLNKIDSFFSNQIKPLGSSNDATLTEKCSECDKLIPVSEILEHADFHIARKLSKEINKSLISSKVMTPEKATTNGKPSKRKRNKPESREPEKRSKGIAAFFS

Target ORF information:

RefSeq Version	XM_012405442.2
Organism	Athalia rosae(coleseed sawfly)
Definition	Athalia rosae DNA polymerase eta (LOC105688847), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012405442.2

ORF Insert Sequence:

ATGAGGTATG GCGGGAAAAT ATCATCACGT GTAGATTCGC GGAGAAGAAA CTGTTCGCCC 
GCTCAAAAGT CTCTACTCTT CGGCCACATG GACATGGATT GCTTTTTTTG CCAAGTCGAA 
GTGAAGTTAC AACCAGAACT ACAGGGAAAA CCATTAGCTG TTGTACAGTT CAATCAGTGG 
CAATCTGGAG GGATTATTGC TGTTAACTAT GAGGCACGAG ATTGTGGAGT TAGCCGACAC 
ATGCGGCCTG AAGAGGCTAA AAAGAAGTGT CCAGAACTGG TCTTGGCAAC TGTTCCCGGG 
GTTCGCGGCA AAGCTGATAC ATCAAGGTAC AGAATTGCCG GCCGTGAAGT GGTTAATGTA 
CTAAAAAAGC ACAGTAACAT CATAGAACGT GCAAGTGTCG ATGAGGCATA CTTAGATATA 
ACAGAACTTG TTAATCAACA AATGATTTCC AGTCAATCAT TGTCATCAGA AATGATAGAT 
CATCTTCCAA ACACGTATGT CATTGGATAT TCAACAGTGG GAAACACTGA CGAAGCCCAA 
AGACGTAAAG CTGTGGAAAA ATGGATATCC GAAGCTTATG GAGAATTGGG AGACATCCAA 
TCTAGGAGGC TTGCTATAGC TGGTACCATA GTTGAGTCTA TAAGAGCTGA TGTGTATAAA 
ACAACGGGAT ACAGATGTTC TGCCGGGGTT TCGTATAACA AGATCTTGGC AAAGCTTGCT 
TGTGGCCTTC ATAAACCCAA CAGACAGACC ATTTTACCTC TGAGTGGGGT TCCATCCCTT 
TATCAAACAT TGCCTGTGAA GAAGGTGCGA AATCTTGGTG GTAAATTTGG AGATTTGGTC 
ATTGAGTCCT TGGGATGTAA TGTTATGGCA GATTTGTTAT CGTATTCATT AAAGGATCTC 
CAAGTACGTT TTGATGATAA AACAGGTTTG TGGTTGTATA ACATTGCTCG TGGTGTAGAT 
AATGAGCCAG TTACCAACAG GCTCATATCC AAATCGATAG GGAGTTGTAA AAATTTTCCT 
GGAAAGCGGG CTATTGTAAC AAAAGAAACT CTTGAACATT GGGTTATGCA GTTGTCTTTA 
GAAATGAGCG AACGACTAGA TCAAGATATG GAGGAGAACA ATCGCAAACC AACCTTGCTG 
ACAGTGAGTT ACCACTACTA TAAGGACAAT AAAATTATAA ATCAATCCCG ATCTTGCCGA 
TTAACTTCAA ACAAAGCTGA AATTATCATG AAAAACTGTT TGGATATCAT AATTAAATTT 
ACCAAATATC CAATCGTGTT TATGGGAATG TCTGCAAGTA AGTTCATTGA AATTGACAAA 
GAGCAAGCAA ATTTCAGGAA TTTCTTCAAA GCAAATATAG ATGTAAGCAA TAAATTGGTA 
CCCAATTGTA CACTCTTGGC AGAGGATACA GGTGACACTT CTTCATTGGA TACTGAATGC 
CCAAGTAATG AAACTTCATG TAATAATAAA ATGAGTTCTG GTGAATCTGA TATCGATGAA 
GCGCGGAGCA GACCTGTGGG CTCGGAGTGT TCTAAATTTT TTGCTGCAGC TCAACAGGAC 
GATAAAAGTA TCGCATCTAG TAGTCACGGC ATGAAAACTG AAAATAAGGA GGATACTTCT 
AAAATCATAT CTCCAGTATC ATTGCTGAGA GCTGAGAATA AAAAATTAAA ACCAGACAAC 
TTTAAGAAAT CATTTTTCAT GAATATTCTA ACTTCTAATA GAATTTCGTC AGAGCACGAA 
TCACCGGGTG GATCTGGTTT TGATGTTACA AGTTGCAGTG GGGAGCTATC TGCTGAAAAT 
GCAAAGCCTG AAAAAAATCC AAATGAAACC TCTGGTGATG TTCTACCACA AGAACTTCAA 
GATTCGGAAA ACTTTAAAAA TCAAAAGGAT GTGAAGAAAA AGGATTCTAG TTCAATTAAT 
ACCATAGAAC CAGAGGAACT TTTTCGCTCT ACCAATTGTA CAAGTCAAAC AGAAGAATCC 
GAATTTTTAC TGAGTAATAG AACTGATGTT GATGACTGTG ACGACAACTC TATAAAACTG 
CAAGATATAT TTCCAGATTT AAATAACCTT GATGAGAGCG TTGTAGCAGT TCTACCTCAG 
AGGCTACAAC GAGAAGCTAA GGCAATTTTG CAAAGTCGAA GTAAAGGCAA TCTAGAAAGT 
AAACTGAATA CAGTGAAAGT AAAGGAGCAG AAAAGTAAAG AGCAGAAGCG ATCACGCGGA 
AAAGTGACAG GTCAAACACG TCTGAATAAA ATAGATTCAT TTTTTAGCAA TCAAATTAAA 
CCGTTAGGAA GTTCTAACGA TGCAACCTTG ACAGAAAAAT GTTCCGAGTG CGATAAATTA 
ATCCCAGTTA GTGAAATCCT GGAACATGCA GATTTTCACA TAGCACGAAA ATTATCCAAA 
GAGATTAATA AATCTTTGAT CAGCTCCAAG GTGATGACTC CAGAAAAAGC GACCACAAAT 
GGTAAGCCAT CAAAGAGAAA ACGTAATAAG CCTGAAAGTC GAGAACCAGA AAAACGATCT 
AAGGGCATAG CAGCATTTTT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105688847 ( XM_012405443.2 ) cDNA ORF clone, Athalia rosae(coleseed sawfly) -> XP_012260866.1 Athalia rosae DNA polymerase eta (LOC105688847), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAt104357
Clone ID Related Accession (Same CDS sequence)	XM_012405443.2
Accession Version	XM_012405443.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-16
Organism	Athalia rosae(coleseed sawfly)
Product	DNA polymerase eta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020311971.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 20, 2017 this sequence version replaced XM_012405443.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Athalia rosae Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATC CAGATAATCG AATTGTCGTT TTAGTGGACA TGGATTGCTT TTTTTGCCAA 
GTCGAAGTGA AGTTACAACC AGAACTACAG GGAAAACCAT TAGCTGTTGT ACAGTTCAAT 
CAGTGGCAAT CTGGAGGGAT TATTGCTGTT AACTATGAGG CACGAGATTG TGGAGTTAGC 
CGACACATGC GGCCTGAAGA GGCTAAAAAG AAGTGTCCAG AACTGGTCTT GGCAACTGTT 
CCCGGGGTTC GCGGCAAAGC TGATACATCA AGGTACAGAA TTGCCGGCCG TGAAGTGGTT 
AATGTACTAA AAAAGCACAG TAACATCATA GAACGTGCAA GTGTCGATGA GGCATACTTA 
GATATAACAG AACTTGTTAA TCAACAAATG ATTTCCAGTC AATCATTGTC ATCAGAAATG 
ATAGATCATC TTCCAAACAC GTATGTCATT GGATATTCAA CAGTGGGAAA CACTGACGAA 
GCCCAAAGAC GTAAAGCTGT GGAAAAATGG ATATCCGAAG CTTATGGAGA ATTGGGAGAC 
ATCCAATCTA GGAGGCTTGC TATAGCTGGT ACCATAGTTG AGTCTATAAG AGCTGATGTG 
TATAAAACAA CGGGATACAG ATGTTCTGCC GGGGTTTCGT ATAACAAGAT CTTGGCAAAG 
CTTGCTTGTG GCCTTCATAA ACCCAACAGA CAGACCATTT TACCTCTGAG TGGGGTTCCA 
TCCCTTTATC AAACATTGCC TGTGAAGAAG GTGCGAAATC TTGGTGGTAA ATTTGGAGAT 
TTGGTCATTG AGTCCTTGGG ATGTAATGTT ATGGCAGATT TGTTATCGTA TTCATTAAAG 
GATCTCCAAG TACGTTTTGA TGATAAAACA GGTTTGTGGT TGTATAACAT TGCTCGTGGT 
GTAGATAATG AGCCAGTTAC CAACAGGCTC ATATCCAAAT CGATAGGGAG TTGTAAAAAT 
TTTCCTGGAA AGCGGGCTAT TGTAACAAAA GAAACTCTTG AACATTGGGT TATGCAGTTG 
TCTTTAGAAA TGAGCGAACG ACTAGATCAA GATATGGAGG AGAACAATCG CAAACCAACC 
TTGCTGACAG TGAGTTACCA CTACTATAAG GACAATAAAA TTATAAATCA ATCCCGATCT 
TGCCGATTAA CTTCAAACAA AGCTGAAATT ATCATGAAAA ACTGTTTGGA TATCATAATT 
AAATTTACCA AATATCCAAT CGTGTTTATG GGAATGTCTG CAAGTAAGTT CATTGAAATT 
GACAAAGAGC AAGCAAATTT CAGGAATTTC TTCAAAGCAA ATATAGATGT AAGCAATAAA 
TTGGTACCCA ATTGTACACT CTTGGCAGAG GATACAGGTG ACACTTCTTC ATTGGATACT 
GAATGCCCAA GTAATGAAAC TTCATGTAAT AATAAAATGA GTTCTGGTGA ATCTGATATC 
GATGAAGCGC GGAGCAGACC TGTGGGCTCG GAGTGTTCTA AATTTTTTGC TGCAGCTCAA 
CAGGACGATA AAAGTATCGC ATCTAGTAGT CACGGCATGA AAACTGAAAA TAAGGAGGAT 
ACTTCTAAAA TCATATCTCC AGTATCATTG CTGAGAGCTG AGAATAAAAA ATTAAAACCA 
GACAACTTTA AGAAATCATT TTTCATGAAT ATTCTAACTT CTAATAGAAT TTCGTCAGAG 
CACGAATCAC CGGGTGGATC TGGTTTTGAT GTTACAAGTT GCAGTGGGGA GCTATCTGCT 
GAAAATGCAA AGCCTGAAAA AAATCCAAAT GAAACCTCTG GTGATGTTCT ACCACAAGAA 
CTTCAAGATT CGGAAAACTT TAAAAATCAA AAGGATGTGA AGAAAAAGGA TTCTAGTTCA 
ATTAATACCA TAGAACCAGA GGAACTTTTT CGCTCTACCA ATTGTACAAG TCAAACAGAA 
GAATCCGAAT TTTTACTGAG TAATAGAACT GATGTTGATG ACTGTGACGA CAACTCTATA 
AAACTGCAAG ATATATTTCC AGATTTAAAT AACCTTGATG AGAGCGTTGT AGCAGTTCTA 
CCTCAGAGGC TACAACGAGA AGCTAAGGCA ATTTTGCAAA GTCGAAGTAA AGGCAATCTA 
GAAAGTAAAC TGAATACAGT GAAAGTAAAG GAGCAGAAAA GTAAAGAGCA GAAGCGATCA 
CGCGGAAAAG TGACAGGTCA AACACGTCTG AATAAAATAG ATTCATTTTT TAGCAATCAA 
ATTAAACCGT TAGGAAGTTC TAACGATGCA ACCTTGACAG AAAAATGTTC CGAGTGCGAT 
AAATTAATCC CAGTTAGTGA AATCCTGGAA CATGCAGATT TTCACATAGC ACGAAAATTA 
TCCAAAGAGA TTAATAAATC TTTGATCAGC TCCAAGGTGA TGACTCCAGA AAAAGCGACC 
ACAAATGGTA AGCCATCAAA GAGAAAACGT AATAAGCCTG AAAGTCGAGA ACCAGAAAAA 
CGATCTAAGG GCATAGCAGC ATTTTTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012260866.1
CDS	136..2628
Translation	MANPDNRIVVLVDMDCFFCQVEVKLQPELQGKPLAVVQFNQWQSGGIIAVNYEARDCGVSRHMRPEEAKKKCPELVLATVPGVRGKADTSRYRIAGREVVNVLKKHSNIIERASVDEAYLDITELVNQQMISSQSLSSEMIDHLPNTYVIGYSTVGNTDEAQRRKAVEKWISEAYGELGDIQSRRLAIAGTIVESIRADVYKTTGYRCSAGVSYNKILAKLACGLHKPNRQTILPLSGVPSLYQTLPVKKVRNLGGKFGDLVIESLGCNVMADLLSYSLKDLQVRFDDKTGLWLYNIARGVDNEPVTNRLISKSIGSCKNFPGKRAIVTKETLEHWVMQLSLEMSERLDQDMEENNRKPTLLTVSYHYYKDNKIINQSRSCRLTSNKAEIIMKNCLDIIIKFTKYPIVFMGMSASKFIEIDKEQANFRNFFKANIDVSNKLVPNCTLLAEDTGDTSSLDTECPSNETSCNNKMSSGESDIDEARSRPVGSECSKFFAAAQQDDKSIASSSHGMKTENKEDTSKIISPVSLLRAENKKLKPDNFKKSFFMNILTSNRISSEHESPGGSGFDVTSCSGELSAENAKPEKNPNETSGDVLPQELQDSENFKNQKDVKKKDSSSINTIEPEELFRSTNCTSQTEESEFLLSNRTDVDDCDDNSIKLQDIFPDLNNLDESVVAVLPQRLQREAKAILQSRSKGNLESKLNTVKVKEQKSKEQKRSRGKVTGQTRLNKIDSFFSNQIKPLGSSNDATLTEKCSECDKLIPVSEILEHADFHIARKLSKEINKSLISSKVMTPEKATTNGKPSKRKRNKPESREPEKRSKGIAAFFS

Target ORF information:

RefSeq Version	XM_012405443.2
Organism	Athalia rosae(coleseed sawfly)
Definition	Athalia rosae DNA polymerase eta (LOC105688847), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012405443.2

ORF Insert Sequence:

ATGGCAAATC CAGATAATCG AATTGTCGTT TTAGTGGACA TGGATTGCTT TTTTTGCCAA 
GTCGAAGTGA AGTTACAACC AGAACTACAG GGAAAACCAT TAGCTGTTGT ACAGTTCAAT 
CAGTGGCAAT CTGGAGGGAT TATTGCTGTT AACTATGAGG CACGAGATTG TGGAGTTAGC 
CGACACATGC GGCCTGAAGA GGCTAAAAAG AAGTGTCCAG AACTGGTCTT GGCAACTGTT 
CCCGGGGTTC GCGGCAAAGC TGATACATCA AGGTACAGAA TTGCCGGCCG TGAAGTGGTT 
AATGTACTAA AAAAGCACAG TAACATCATA GAACGTGCAA GTGTCGATGA GGCATACTTA 
GATATAACAG AACTTGTTAA TCAACAAATG ATTTCCAGTC AATCATTGTC ATCAGAAATG 
ATAGATCATC TTCCAAACAC GTATGTCATT GGATATTCAA CAGTGGGAAA CACTGACGAA 
GCCCAAAGAC GTAAAGCTGT GGAAAAATGG ATATCCGAAG CTTATGGAGA ATTGGGAGAC 
ATCCAATCTA GGAGGCTTGC TATAGCTGGT ACCATAGTTG AGTCTATAAG AGCTGATGTG 
TATAAAACAA CGGGATACAG ATGTTCTGCC GGGGTTTCGT ATAACAAGAT CTTGGCAAAG 
CTTGCTTGTG GCCTTCATAA ACCCAACAGA CAGACCATTT TACCTCTGAG TGGGGTTCCA 
TCCCTTTATC AAACATTGCC TGTGAAGAAG GTGCGAAATC TTGGTGGTAA ATTTGGAGAT 
TTGGTCATTG AGTCCTTGGG ATGTAATGTT ATGGCAGATT TGTTATCGTA TTCATTAAAG 
GATCTCCAAG TACGTTTTGA TGATAAAACA GGTTTGTGGT TGTATAACAT TGCTCGTGGT 
GTAGATAATG AGCCAGTTAC CAACAGGCTC ATATCCAAAT CGATAGGGAG TTGTAAAAAT 
TTTCCTGGAA AGCGGGCTAT TGTAACAAAA GAAACTCTTG AACATTGGGT TATGCAGTTG 
TCTTTAGAAA TGAGCGAACG ACTAGATCAA GATATGGAGG AGAACAATCG CAAACCAACC 
TTGCTGACAG TGAGTTACCA CTACTATAAG GACAATAAAA TTATAAATCA ATCCCGATCT 
TGCCGATTAA CTTCAAACAA AGCTGAAATT ATCATGAAAA ACTGTTTGGA TATCATAATT 
AAATTTACCA AATATCCAAT CGTGTTTATG GGAATGTCTG CAAGTAAGTT CATTGAAATT 
GACAAAGAGC AAGCAAATTT CAGGAATTTC TTCAAAGCAA ATATAGATGT AAGCAATAAA 
TTGGTACCCA ATTGTACACT CTTGGCAGAG GATACAGGTG ACACTTCTTC ATTGGATACT 
GAATGCCCAA GTAATGAAAC TTCATGTAAT AATAAAATGA GTTCTGGTGA ATCTGATATC 
GATGAAGCGC GGAGCAGACC TGTGGGCTCG GAGTGTTCTA AATTTTTTGC TGCAGCTCAA 
CAGGACGATA AAAGTATCGC ATCTAGTAGT CACGGCATGA AAACTGAAAA TAAGGAGGAT 
ACTTCTAAAA TCATATCTCC AGTATCATTG CTGAGAGCTG AGAATAAAAA ATTAAAACCA 
GACAACTTTA AGAAATCATT TTTCATGAAT ATTCTAACTT CTAATAGAAT TTCGTCAGAG 
CACGAATCAC CGGGTGGATC TGGTTTTGAT GTTACAAGTT GCAGTGGGGA GCTATCTGCT 
GAAAATGCAA AGCCTGAAAA AAATCCAAAT GAAACCTCTG GTGATGTTCT ACCACAAGAA 
CTTCAAGATT CGGAAAACTT TAAAAATCAA AAGGATGTGA AGAAAAAGGA TTCTAGTTCA 
ATTAATACCA TAGAACCAGA GGAACTTTTT CGCTCTACCA ATTGTACAAG TCAAACAGAA 
GAATCCGAAT TTTTACTGAG TAATAGAACT GATGTTGATG ACTGTGACGA CAACTCTATA 
AAACTGCAAG ATATATTTCC AGATTTAAAT AACCTTGATG AGAGCGTTGT AGCAGTTCTA 
CCTCAGAGGC TACAACGAGA AGCTAAGGCA ATTTTGCAAA GTCGAAGTAA AGGCAATCTA 
GAAAGTAAAC TGAATACAGT GAAAGTAAAG GAGCAGAAAA GTAAAGAGCA GAAGCGATCA 
CGCGGAAAAG TGACAGGTCA AACACGTCTG AATAAAATAG ATTCATTTTT TAGCAATCAA 
ATTAAACCGT TAGGAAGTTC TAACGATGCA ACCTTGACAG AAAAATGTTC CGAGTGCGAT 
AAATTAATCC CAGTTAGTGA AATCCTGGAA CATGCAGATT TTCACATAGC ACGAAAATTA 
TCCAAAGAGA TTAATAAATC TTTGATCAGC TCCAAGGTGA TGACTCCAGA AAAAGCGACC 
ACAAATGGTA AGCCATCAAA GAGAAAACGT AATAAGCCTG AAAGTCGAGA ACCAGAAAAA 
CGATCTAAGG GCATAGCAGC ATTTTTTTCC TGA