LOC105686875 cDNA ORF clone, Athalia rosae(coleseed sawfly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105686875
Entrez Gene ID	105686875
Full Name	sphingomyelin phosphodiesterase
Gene Type	protein-coding
Organism	Athalia rosae(coleseed sawfly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012402085.2	XP_012257508.1	sphingomyelin phosphodiesterase

Pathways from BioSystems
Gene Ontology

The following LOC105686875 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105686875 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAt101644	XM_012402085.2 Latest version!	Athalia rosae sphingomyelin phosphodiesterase (LOC105686875), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105686875 ( XM_012402085.2 ) cDNA ORF clone, Athalia rosae(coleseed sawfly) -> XP_012257508.1 Athalia rosae sphingomyelin phosphodiesterase (LOC105686875), transcript variant X1, mRNA.

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CloneID	OAt101644
Clone ID Related Accession (Same CDS sequence)	XM_012402085.2
Accession Version	XM_012402085.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-16
Organism	Athalia rosae(coleseed sawfly)
Product	sphingomyelin phosphodiesterase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020311981.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 20, 2017 this sequence version replaced XM_012402085.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Athalia rosae Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAATC AAAAATGGGC TCTGAAAAAA TGCAGTGGTC TTATCCTACT ATGCTTAGCC 
ACCACGCAGT GGCTGCAGAC GTGCGGAATT CCCGTGAGAG AAAGATCGAA AAGAGAAGCG 
GAGATTGCGA ATACAAATTG GACCGGACTT GCGGATGAAT GGTTGATGGC AAAGGAAAGG 
ATAGCGAACA GGATGGGAAT CCCTTTACTT CCAAATAGCA ATACGACGCA CGAAGTTCCA 
ATAGTTGAAA TTGTCGTAAA CGGTACAAAT AATGACCCCG AGCAACTTTC GCGGTTCAAT 
TACTCCCGGA TGCTCTGGGA TTTGGAGTCC TCTCAAAAGT CTGGTCATCT TTCGGGATTC 
GTTGACAAGG CACTCAAGCT TTTGGATCTG AGGCAAGTGA TGATGGAAGT TGAGACATCG 
GTAATGTCGA AGGTCTCTTG TACAGCTTGC AAAGTCGGCG CTGGACTTCT TCAGCATTAT 
ATCAAAAGTG GGAAAACTGA GGAAGAAATA ATGGCTAGCA TATATCAATT TTGTGTATCG 
CTGAAGATAC AAAGTCCCCG GGTATGCCAG GGCGTTACGT TGCTTTTTGG AGGTGAGGTC 
ATCTACGTGT TGAAGCAAAT CAGTATGGGG CCATCGCAGA TTTGTAGTTT CGTTATCGGC 
GATGCTTGCG ACGATGCTTA TAATCCTCAA CACGAGTGGG AAGTTGCTTT TCCTCCGGTC 
AACAAACCGC CGATACAACC ACCGATTCCT CCTATCGAAG GAGCACCGAC CTTCAAGGTG 
CTCCACATAT CGGACACCCA CTATGACCCC TATTATCAGG AAGGAACAAA CGCTGATTGC 
AACGAACCTC TTTGCTGTAG ACTAACGAAC GGCGCACCGT TAACGGCATC GGCAGCAGCC 
GGAAGATGGG GGGATTACAG AAAGTGCGAT ACACCCAAGA GAACTGTAGA TCATATGTTG 
AAACACATAG CAGACACACA TTCGGATATA GACTACATCT TATGGACGGG TGATTTACCA 
CCTCACGACG TATGGAACCA AACTAGAGAA GAGAATCTTC GAATTTTACG CGACACCGTT 
ACCCAAATGA TCGAAATGTT CCCTGGAATT CCGATATTTC CTGCTTTAGG AAATCACGAG 
AGTGCTCCCG TAAACAGCTT CCCTCCGCCC TTCGTACCTG AAGAGAACAG TATCTCTTGG 
CTTTATGACG AGCTTGATAA GGAGTGGAGA AGATGGCTAC CTGCGGGAGT ATCTCATACT 
GTAAGACGCG GGGCATTTTA TTCTGTTCTC GTCAGACCAG GGTTCAGAAT ATTGTCGGTG 
AACATGAATT ATTGCAACAA CAAGAACTGG TGGTTACTGA TAAACAGCAC GGATCCGGTC 
AGTGAACTTC AATGGTTGAT TTACGAACTT CAGGGGGCTG AAATAAACGG TGAAAAAGTT 
CACATAATCG GACACATACC GCCAGGACAT TCGGATTGCT TGAAAGTTTG GTCCAGGAAT 
TATTACCACA TAATCAATCG TTACGAGTCG ACAATCACTG CCCAGTTCTT TGGACATACT 
CATTATGATG AGTTTCAGTT ATTCTACGAT ACAACGGACC TTGGAAGGGC CCTTGGCGTC 
GCATACATAG GGCCATCAGT TTCACCTTAC ATTGATCTGA ACCCCGGATA CCGAATATAC 
TACGTCGATG GCGATCATGC GAAAACTACT AGGATGGTCG TCGAGCACGA GTCGTGGGTG 
ATGAATCTGA AAGAAGCAAA TCTATACGAC TATCCAATAT GGCAAAAAAT GTACACAGCT 
CGTCAAGCCT ACCAGATGGC ATCGCTTTTG CCCCGTGATT GGGACTCTTT GATCGATAAA 
ATGACTAACG AACCGTCGCT TTTTGACCTT TACTACAAGC ACTACAACAA AAATTCTCCG 
GTCAGACCCG CGTGTAATGA CGAGTGTCGC AAGCGTTTAC TTTGCGATTT ACGAAGCGGT 
AGGAGTCACG ATAGAAAAGC ACTTTGTCAG AGTTTGGAAT CCAGAATAGA CGGAGAAACT 
AGAACTGGAT GGAGAGCTTG GATATACAAC GGTCTAGCAC TATCGGTATC AGTTGTCATG 
GCGATTCCAA GATTTGCTTA CAACTTACCG AAATATGTCC TGGGTCTCGG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012257508.1
CDS	782..2935
Translation	MPNQKWALKKCSGLILLCLATTQWLQTCGIPVRERSKREAEIANTNWTGLADEWLMAKERIANRMGIPLLPNSNTTHEVPIVEIVVNGTNNDPEQLSRFNYSRMLWDLESSQKSGHLSGFVDKALKLLDLRQVMMEVETSVMSKVSCTACKVGAGLLQHYIKSGKTEEEIMASIYQFCVSLKIQSPRVCQGVTLLFGGEVIYVLKQISMGPSQICSFVIGDACDDAYNPQHEWEVAFPPVNKPPIQPPIPPIEGAPTFKVLHISDTHYDPYYQEGTNADCNEPLCCRLTNGAPLTASAAAGRWGDYRKCDTPKRTVDHMLKHIADTHSDIDYILWTGDLPPHDVWNQTREENLRILRDTVTQMIEMFPGIPIFPALGNHESAPVNSFPPPFVPEENSISWLYDELDKEWRRWLPAGVSHTVRRGAFYSVLVRPGFRILSVNMNYCNNKNWWLLINSTDPVSELQWLIYELQGAEINGEKVHIIGHIPPGHSDCLKVWSRNYYHIINRYESTITAQFFGHTHYDEFQLFYDTTDLGRALGVAYIGPSVSPYIDLNPGYRIYYVDGDHAKTTRMVVEHESWVMNLKEANLYDYPIWQKMYTARQAYQMASLLPRDWDSLIDKMTNEPSLFDLYYKHYNKNSPVRPACNDECRKRLLCDLRSGRSHDRKALCQSLESRIDGETRTGWRAWIYNGLALSVSVVMAIPRFAYNLPKYVLGLG

Target ORF information:

RefSeq Version	XM_012402085.2
Organism	Athalia rosae(coleseed sawfly)
Definition	Athalia rosae sphingomyelin phosphodiesterase (LOC105686875), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012402085.2

ORF Insert Sequence:

ATGCCAAATC AAAAATGGGC TCTGAAAAAA TGCAGTGGTC TTATCCTACT ATGCTTAGCC 
ACCACGCAGT GGCTGCAGAC GTGCGGAATT CCCGTGAGAG AAAGATCGAA AAGAGAAGCG 
GAGATTGCGA ATACAAATTG GACCGGACTT GCGGATGAAT GGTTGATGGC AAAGGAAAGG 
ATAGCGAACA GGATGGGAAT CCCTTTACTT CCAAATAGCA ATACGACGCA CGAAGTTCCA 
ATAGTTGAAA TTGTCGTAAA CGGTACAAAT AATGACCCCG AGCAACTTTC GCGGTTCAAT 
TACTCCCGGA TGCTCTGGGA TTTGGAGTCC TCTCAAAAGT CTGGTCATCT TTCGGGATTC 
GTTGACAAGG CACTCAAGCT TTTGGATCTG AGGCAAGTGA TGATGGAAGT TGAGACATCG 
GTAATGTCGA AGGTCTCTTG TACAGCTTGC AAAGTCGGCG CTGGACTTCT TCAGCATTAT 
ATCAAAAGTG GGAAAACTGA GGAAGAAATA ATGGCTAGCA TATATCAATT TTGTGTATCG 
CTGAAGATAC AAAGTCCCCG GGTATGCCAG GGCGTTACGT TGCTTTTTGG AGGTGAGGTC 
ATCTACGTGT TGAAGCAAAT CAGTATGGGG CCATCGCAGA TTTGTAGTTT CGTTATCGGC 
GATGCTTGCG ACGATGCTTA TAATCCTCAA CACGAGTGGG AAGTTGCTTT TCCTCCGGTC 
AACAAACCGC CGATACAACC ACCGATTCCT CCTATCGAAG GAGCACCGAC CTTCAAGGTG 
CTCCACATAT CGGACACCCA CTATGACCCC TATTATCAGG AAGGAACAAA CGCTGATTGC 
AACGAACCTC TTTGCTGTAG ACTAACGAAC GGCGCACCGT TAACGGCATC GGCAGCAGCC 
GGAAGATGGG GGGATTACAG AAAGTGCGAT ACACCCAAGA GAACTGTAGA TCATATGTTG 
AAACACATAG CAGACACACA TTCGGATATA GACTACATCT TATGGACGGG TGATTTACCA 
CCTCACGACG TATGGAACCA AACTAGAGAA GAGAATCTTC GAATTTTACG CGACACCGTT 
ACCCAAATGA TCGAAATGTT CCCTGGAATT CCGATATTTC CTGCTTTAGG AAATCACGAG 
AGTGCTCCCG TAAACAGCTT CCCTCCGCCC TTCGTACCTG AAGAGAACAG TATCTCTTGG 
CTTTATGACG AGCTTGATAA GGAGTGGAGA AGATGGCTAC CTGCGGGAGT ATCTCATACT 
GTAAGACGCG GGGCATTTTA TTCTGTTCTC GTCAGACCAG GGTTCAGAAT ATTGTCGGTG 
AACATGAATT ATTGCAACAA CAAGAACTGG TGGTTACTGA TAAACAGCAC GGATCCGGTC 
AGTGAACTTC AATGGTTGAT TTACGAACTT CAGGGGGCTG AAATAAACGG TGAAAAAGTT 
CACATAATCG GACACATACC GCCAGGACAT TCGGATTGCT TGAAAGTTTG GTCCAGGAAT 
TATTACCACA TAATCAATCG TTACGAGTCG ACAATCACTG CCCAGTTCTT TGGACATACT 
CATTATGATG AGTTTCAGTT ATTCTACGAT ACAACGGACC TTGGAAGGGC CCTTGGCGTC 
GCATACATAG GGCCATCAGT TTCACCTTAC ATTGATCTGA ACCCCGGATA CCGAATATAC 
TACGTCGATG GCGATCATGC GAAAACTACT AGGATGGTCG TCGAGCACGA GTCGTGGGTG 
ATGAATCTGA AAGAAGCAAA TCTATACGAC TATCCAATAT GGCAAAAAAT GTACACAGCT 
CGTCAAGCCT ACCAGATGGC ATCGCTTTTG CCCCGTGATT GGGACTCTTT GATCGATAAA 
ATGACTAACG AACCGTCGCT TTTTGACCTT TACTACAAGC ACTACAACAA AAATTCTCCG 
GTCAGACCCG CGTGTAATGA CGAGTGTCGC AAGCGTTTAC TTTGCGATTT ACGAAGCGGT 
AGGAGTCACG ATAGAAAAGC ACTTTGTCAG AGTTTGGAAT CCAGAATAGA CGGAGAAACT 
AGAACTGGAT GGAGAGCTTG GATATACAAC GGTCTAGCAC TATCGGTATC AGTTGTCATG 
GCGATTCCAA GATTTGCTTA CAACTTACCG AAATATGTCC TGGGTCTCGG GTAA