LOC105686557 cDNA ORF clone, Athalia rosae(coleseed sawfly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105686557
Entrez Gene ID	105686557
Full Name	helicase SKI2W
Gene Type	protein-coding
Organism	Athalia rosae(coleseed sawfly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012401491.2	XP_012256914.1	helicase SKI2W

Pathways from BioSystems
Gene Ontology

The following LOC105686557 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105686557 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAt100966	XM_012401491.2 Latest version!	Athalia rosae helicase SKI2W (LOC105686557), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105686557 ( XM_012401491.2 ) cDNA ORF clone, Athalia rosae(coleseed sawfly) -> XP_012256914.1 Athalia rosae helicase SKI2W (LOC105686557), mRNA.

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CloneID	OAt100966
Clone ID Related Accession (Same CDS sequence)	XM_012401491.2
Accession Version	XM_012401491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-16
Organism	Athalia rosae(coleseed sawfly)
Product	helicase SKI2W
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020311966.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 20, 2017 this sequence version replaced XM_012401491.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Athalia rosae Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAG TACGTGCGGA TTTTCAGCGG GACCTACACA TCAACTCTCC ACCAGTTCTC 
TGTAACCTGG AAAATCTTCT TCAAGGGTAT ATAGAAGGTC CAGAACAGTT GCCTATTCAC 
GATGTCCAAA AGATTCAGTC CTACTGGCCT AGGCAGCCAA ACATTTTTGC GCTTCTTCAA 
TCAGATCTGG CACCGATTGA AACGTACATT AAATTCAAAA GGGACACAAT AACCGGGTTA 
TTCGGTGATA TGTATGAAGA CTACATACAA GGTGCTGGCG CCACGCCTAG GAACTCGCTG 
TCTATGCGTC GAACTCCTGT CCTACAAGGA GTGAGAGGTA ACGCGAGCAA CTTTCTGTTT 
TGGCCCGGTG GATTCGATGT GCCTGAGCCA ACGCAGGATG GTCCTAGTGT GGAAATCAAT 
TTTGAGAACA ATCTAAGGGT ATTGGCAAAA GGTTTCAAGT CTGGAATAAA ATTTTCGGAA 
GATAAGTGTT CACCTAAGAA TGCTAACAAA GCAGATATCA CCAACCCAGA TTCTCTCCCG 
CATCAGAAAG AGATAGAACC CTGCAGAAAG ACCATAAATT TGATGGCCAT GGTACACGAG 
CAGGAGAATT TGATCGGCTT ATGGTCCATG GCTGAGGTAA ACACACCCAA AGAAGATGAG 
CCTCTCAAAT CGTCTCTTGC AGAATTGCCT GATCTAGAAA AAATAGTCCG AAAGCATTCT 
GCGTTGGCTG ATTTGCCAGT TTTAGAAATA ACAAAGAAAG TGAACGTTGC TAAGATATCT 
ACCGAATGGG CTGAAGTCTT GGATGTTTCA CTTCCAGTTT CAGACTTTGA AAAACGCATC 
CCAGATCCAG CTATCAAGTA TCCCTATGAG TTGGATACAT TCCAAAAACA GGCGATCCTG 
AAACTTGAAG ACAATTGCAA TGTTTTTATA GCTGCCCATA CTTCCGCGGG AAAAACTACC 
GTTGCCGAGT ACGCTATTGC TTTGAGTCAG AAGCACATGA CCAGGTCTAT TTACACATCT 
CCTATAAAAG CCTTGTCAAA TCAAAAATAC CGTGATTTCA AAAAGACATT TGAAAGTGTA 
GGACTAATTA CTGGAGATCT ACAAATTAAT CCAACGGCAT CATGCCTCAT TATGACAACC 
GAAATCTTGC AATCAATGCT GTATTGTGCC TCAGAAGTAG TCAGAGACCT GGAGTATGTG 
ATATTTGATG AAGTTCACTA TATAAACAAC GAGGATCGTG GCCATGTCTG GGAAGAAGTA 
TTGATCCTTT TACCACAAAA TGTGAGCATA GTAATGTTGT CTGCAACTGT TCCCAATACC 
TTGGAATTTG CAGATTGGGT TGGTAGAACT AAAAAACGGA AAGTTTACGT TATAAGTACT 
CTTAAACGTC CGGTGCCGCT GCAGCATTAC TTGTATACCG GCTGCGGTGG TAAAAGCAAA 
AACGATCGAT TTTTGATAGT CGACTCGGAT GGAAAATTCA AAAAGGATGG CTGGGAAAAA 
GCGTCAGCGG CTAAGGCAGC AAACACGAAA AACCCAGGTG AAAGGAAGTA TCAGCTGAAT 
CCAAATCAGG AAAAAACCTT GTGGATCGGC TTTGTAGATC ATCTCAAGAG AGAAAACAAG 
TTACCAGTCG TGGCCTTCAC TTTATCGAGA AATCGCTGCG ACCAAAATGC GGATGCTATG 
AGATCCGTTG ATTTGACAAC AGCAACGGAA AAACATCAAA TCAGAAAATT TTTTCAGCAA 
TGCATAACTC GGTTAAAAGA ACCAGACAGA GAATTGCCAC AAATAATAAA CATGCAACAA 
CTATTAGAGC GAGGAATAGG CGTGCACCAT AGCGGAATTT TACCATTCTT GAAAGAAATA 
GTCGAACTAT TATTTCAAAC TGGAGTTGTG AAGTTACTAT TTGCCACGGA AACTTTTGCA 
ATGGGTGTAA ACATGCCTGC GCGTACTGTG GTCTTTGATT CCGTGAGAAA ATTTGACGGT 
ACAGAGTTGC GAACTCTCCA TCCAGCTGAA TATATTCAAA TGGCGGGTCG TGCCGGGCGT 
AGAGGCTTGG ATGACACGGG AACAGTGATA ATTCTTTGCA AAACAGACGT GCCGGATACG 
CTGGTACTAC AAAATATGAT GTTGGGTAAG CCACAGAAGT TGGAATCTCA ATTCAGGATG 
ACGTACGCTA TGATATTGAA CTTGAGAAGG GTGACCGAGT CCGTCACTAT AGAGGGAATG 
ATGCGCCGAT CTTTCAAGGA GGTCGCACTT GTTGCTCATG AGAAAAAGTA TAAGGCCGAA 
CTTGCGGAGG TAGAAAAACA GCTTTCAAAC TGCGCTGATT TATCAGACTC ACAAAAGCAG 
CTCTGCAATT TCTACTCGAT TGCCACGGAG TATCTAGAAG AATGGTTCCA ATTGAGACCC 
TATCTTTTAG ACACGAAGAA GGCTGCCAAA TATTTAACAC CCGGTAGAAT TGTACTAGTA 
TCGCACCGCG AACACTACAA TAAACTTGCT TTACTCTTGG CTACAATACA TCGGCGGCAG 
GACACACTTT ACAGAGTTCT GTTACTTACC AATATTCGGC AATCTGATTC TGAACAAGGC 
ATAAATCCAG AAGTCATGGA ATCGAAAATT GGTGAAGAAA AGTCGGACAA CTGGTACAAG 
ATTATTGGGT TCAGCAAGAA AGAACTATTT GTACCTGAGG GAGTTGCTGG TCACCAGATT 
CTTGTCATTT CAGCTTCGGA TATTCTAGAA ATTACTTCAC GAGTGATGAA GATTGATTGT 
CAGCTCATCA TAAGTGACTG GGAAAAGAGG CAAATCCCCA GATTCAAGAA CGATCCCCCG 
GGTCGCACAT GTTTAGCTGC TATTCAAGAG CTGACAACGA TATCATTAGA CGCGGTCACG 
AACCACGAAA CGATCGAGCC TTTCGTAGAT TTAAAATTGA ATGAACCGTC TTTAGCTCCT 
AGAATGCAGT ACCTAAAACA ATTGAAAGAT AAATTGGATA AAATAACGTG TACGCACATT 
GCCAATTTAG AGGAACAGTT TGAAGCTGTA TTCACTAAAA AAGAATTGGA AGCTAGAAGA 
GAAAGACTCA GCTTCCGCCT CAGCCACGAA AGTCTGTCAC TCTACCCAGA GTATACAAAC 
AAGGTTGCCG TCCTCAAAAC ATTACGCTAC ATTGACGATG ACGAAAGAGT TGCACTTAAA 
GGCCGAGTGG CCCTGGAAAT GGGGACACAT GAATTGCTCA TGACTGAACT AGTCTTCACA 
AACGCTCTGA CAGCGCTGCA GCCAGCTGAA ATTGCTGCTC TACTTTCTGC CCTAGTTTTT 
CAGCAAAAGA CTAATGTGGA ACCGCAGCTG ATCTCATCTC TGAAAAACGG ATGTAAAATC 
ATAGAAAAGG TTCAAAACAG CATTGCATTG GTGGAACAAC AAAACGCCGT TGACGTGGAA 
GAGCCATTAA ATTTCGGATT AGTGCAAGTT GTTTACGAAT GGGCAAGGTC AGAACCGTTC 
GCCAAAATAA TGGAACTGAC AGACGTCCAA GAAGGTATAA TCGTAAGATG TATACAGCAG 
TTAAATGAAA CCCTGAGATA TGTAAAAAAC GCTGCTATCA TTATTGGCGA CCCAGTGCTG 
AAGGAGAAGA TGGAAGAAGC ATCTTCTGCT ATAAGAAGAG ACATAGTTTT CGCTCAATCC 
TTCTATACCC AAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012256914.1
CDS	92..3769
Translation	MNTVRADFQRDLHINSPPVLCNLENLLQGYIEGPEQLPIHDVQKIQSYWPRQPNIFALLQSDLAPIETYIKFKRDTITGLFGDMYEDYIQGAGATPRNSLSMRRTPVLQGVRGNASNFLFWPGGFDVPEPTQDGPSVEINFENNLRVLAKGFKSGIKFSEDKCSPKNANKADITNPDSLPHQKEIEPCRKTINLMAMVHEQENLIGLWSMAEVNTPKEDEPLKSSLAELPDLEKIVRKHSALADLPVLEITKKVNVAKISTEWAEVLDVSLPVSDFEKRIPDPAIKYPYELDTFQKQAILKLEDNCNVFIAAHTSAGKTTVAEYAIALSQKHMTRSIYTSPIKALSNQKYRDFKKTFESVGLITGDLQINPTASCLIMTTEILQSMLYCASEVVRDLEYVIFDEVHYINNEDRGHVWEEVLILLPQNVSIVMLSATVPNTLEFADWVGRTKKRKVYVISTLKRPVPLQHYLYTGCGGKSKNDRFLIVDSDGKFKKDGWEKASAAKAANTKNPGERKYQLNPNQEKTLWIGFVDHLKRENKLPVVAFTLSRNRCDQNADAMRSVDLTTATEKHQIRKFFQQCITRLKEPDRELPQIINMQQLLERGIGVHHSGILPFLKEIVELLFQTGVVKLLFATETFAMGVNMPARTVVFDSVRKFDGTELRTLHPAEYIQMAGRAGRRGLDDTGTVIILCKTDVPDTLVLQNMMLGKPQKLESQFRMTYAMILNLRRVTESVTIEGMMRRSFKEVALVAHEKKYKAELAEVEKQLSNCADLSDSQKQLCNFYSIATEYLEEWFQLRPYLLDTKKAAKYLTPGRIVLVSHREHYNKLALLLATIHRRQDTLYRVLLLTNIRQSDSEQGINPEVMESKIGEEKSDNWYKIIGFSKKELFVPEGVAGHQILVISASDILEITSRVMKIDCQLIISDWEKRQIPRFKNDPPGRTCLAAIQELTTISLDAVTNHETIEPFVDLKLNEPSLAPRMQYLKQLKDKLDKITCTHIANLEEQFEAVFTKKELEARRERLSFRLSHESLSLYPEYTNKVAVLKTLRYIDDDERVALKGRVALEMGTHELLMTELVFTNALTALQPAEIAALLSALVFQQKTNVEPQLISSLKNGCKIIEKVQNSIALVEQQNAVDVEEPLNFGLVQVVYEWARSEPFAKIMELTDVQEGIIVRCIQQLNETLRYVKNAAIIIGDPVLKEKMEEASSAIRRDIVFAQSFYTQD

Target ORF information:

RefSeq Version	XM_012401491.2
Organism	Athalia rosae(coleseed sawfly)
Definition	Athalia rosae helicase SKI2W (LOC105686557), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012401491.2

ORF Insert Sequence:

ATGAACACAG TACGTGCGGA TTTTCAGCGG GACCTACACA TCAACTCTCC ACCAGTTCTC 
TGTAACCTGG AAAATCTTCT TCAAGGGTAT ATAGAAGGTC CAGAACAGTT GCCTATTCAC 
GATGTCCAAA AGATTCAGTC CTACTGGCCT AGGCAGCCAA ACATTTTTGC GCTTCTTCAA 
TCAGATCTGG CACCGATTGA AACGTACATT AAATTCAAAA GGGACACAAT AACCGGGTTA 
TTCGGTGATA TGTATGAAGA CTACATACAA GGTGCTGGCG CCACGCCTAG GAACTCGCTG 
TCTATGCGTC GAACTCCTGT CCTACAAGGA GTGAGAGGTA ACGCGAGCAA CTTTCTGTTT 
TGGCCCGGTG GATTCGATGT GCCTGAGCCA ACGCAGGATG GTCCTAGTGT GGAAATCAAT 
TTTGAGAACA ATCTAAGGGT ATTGGCAAAA GGTTTCAAGT CTGGAATAAA ATTTTCGGAA 
GATAAGTGTT CACCTAAGAA TGCTAACAAA GCAGATATCA CCAACCCAGA TTCTCTCCCG 
CATCAGAAAG AGATAGAACC CTGCAGAAAG ACCATAAATT TGATGGCCAT GGTACACGAG 
CAGGAGAATT TGATCGGCTT ATGGTCCATG GCTGAGGTAA ACACACCCAA AGAAGATGAG 
CCTCTCAAAT CGTCTCTTGC AGAATTGCCT GATCTAGAAA AAATAGTCCG AAAGCATTCT 
GCGTTGGCTG ATTTGCCAGT TTTAGAAATA ACAAAGAAAG TGAACGTTGC TAAGATATCT 
ACCGAATGGG CTGAAGTCTT GGATGTTTCA CTTCCAGTTT CAGACTTTGA AAAACGCATC 
CCAGATCCAG CTATCAAGTA TCCCTATGAG TTGGATACAT TCCAAAAACA GGCGATCCTG 
AAACTTGAAG ACAATTGCAA TGTTTTTATA GCTGCCCATA CTTCCGCGGG AAAAACTACC 
GTTGCCGAGT ACGCTATTGC TTTGAGTCAG AAGCACATGA CCAGGTCTAT TTACACATCT 
CCTATAAAAG CCTTGTCAAA TCAAAAATAC CGTGATTTCA AAAAGACATT TGAAAGTGTA 
GGACTAATTA CTGGAGATCT ACAAATTAAT CCAACGGCAT CATGCCTCAT TATGACAACC 
GAAATCTTGC AATCAATGCT GTATTGTGCC TCAGAAGTAG TCAGAGACCT GGAGTATGTG 
ATATTTGATG AAGTTCACTA TATAAACAAC GAGGATCGTG GCCATGTCTG GGAAGAAGTA 
TTGATCCTTT TACCACAAAA TGTGAGCATA GTAATGTTGT CTGCAACTGT TCCCAATACC 
TTGGAATTTG CAGATTGGGT TGGTAGAACT AAAAAACGGA AAGTTTACGT TATAAGTACT 
CTTAAACGTC CGGTGCCGCT GCAGCATTAC TTGTATACCG GCTGCGGTGG TAAAAGCAAA 
AACGATCGAT TTTTGATAGT CGACTCGGAT GGAAAATTCA AAAAGGATGG CTGGGAAAAA 
GCGTCAGCGG CTAAGGCAGC AAACACGAAA AACCCAGGTG AAAGGAAGTA TCAGCTGAAT 
CCAAATCAGG AAAAAACCTT GTGGATCGGC TTTGTAGATC ATCTCAAGAG AGAAAACAAG 
TTACCAGTCG TGGCCTTCAC TTTATCGAGA AATCGCTGCG ACCAAAATGC GGATGCTATG 
AGATCCGTTG ATTTGACAAC AGCAACGGAA AAACATCAAA TCAGAAAATT TTTTCAGCAA 
TGCATAACTC GGTTAAAAGA ACCAGACAGA GAATTGCCAC AAATAATAAA CATGCAACAA 
CTATTAGAGC GAGGAATAGG CGTGCACCAT AGCGGAATTT TACCATTCTT GAAAGAAATA 
GTCGAACTAT TATTTCAAAC TGGAGTTGTG AAGTTACTAT TTGCCACGGA AACTTTTGCA 
ATGGGTGTAA ACATGCCTGC GCGTACTGTG GTCTTTGATT CCGTGAGAAA ATTTGACGGT 
ACAGAGTTGC GAACTCTCCA TCCAGCTGAA TATATTCAAA TGGCGGGTCG TGCCGGGCGT 
AGAGGCTTGG ATGACACGGG AACAGTGATA ATTCTTTGCA AAACAGACGT GCCGGATACG 
CTGGTACTAC AAAATATGAT GTTGGGTAAG CCACAGAAGT TGGAATCTCA ATTCAGGATG 
ACGTACGCTA TGATATTGAA CTTGAGAAGG GTGACCGAGT CCGTCACTAT AGAGGGAATG 
ATGCGCCGAT CTTTCAAGGA GGTCGCACTT GTTGCTCATG AGAAAAAGTA TAAGGCCGAA 
CTTGCGGAGG TAGAAAAACA GCTTTCAAAC TGCGCTGATT TATCAGACTC ACAAAAGCAG 
CTCTGCAATT TCTACTCGAT TGCCACGGAG TATCTAGAAG AATGGTTCCA ATTGAGACCC 
TATCTTTTAG ACACGAAGAA GGCTGCCAAA TATTTAACAC CCGGTAGAAT TGTACTAGTA 
TCGCACCGCG AACACTACAA TAAACTTGCT TTACTCTTGG CTACAATACA TCGGCGGCAG 
GACACACTTT ACAGAGTTCT GTTACTTACC AATATTCGGC AATCTGATTC TGAACAAGGC 
ATAAATCCAG AAGTCATGGA ATCGAAAATT GGTGAAGAAA AGTCGGACAA CTGGTACAAG 
ATTATTGGGT TCAGCAAGAA AGAACTATTT GTACCTGAGG GAGTTGCTGG TCACCAGATT 
CTTGTCATTT CAGCTTCGGA TATTCTAGAA ATTACTTCAC GAGTGATGAA GATTGATTGT 
CAGCTCATCA TAAGTGACTG GGAAAAGAGG CAAATCCCCA GATTCAAGAA CGATCCCCCG 
GGTCGCACAT GTTTAGCTGC TATTCAAGAG CTGACAACGA TATCATTAGA CGCGGTCACG 
AACCACGAAA CGATCGAGCC TTTCGTAGAT TTAAAATTGA ATGAACCGTC TTTAGCTCCT 
AGAATGCAGT ACCTAAAACA ATTGAAAGAT AAATTGGATA AAATAACGTG TACGCACATT 
GCCAATTTAG AGGAACAGTT TGAAGCTGTA TTCACTAAAA AAGAATTGGA AGCTAGAAGA 
GAAAGACTCA GCTTCCGCCT CAGCCACGAA AGTCTGTCAC TCTACCCAGA GTATACAAAC 
AAGGTTGCCG TCCTCAAAAC ATTACGCTAC ATTGACGATG ACGAAAGAGT TGCACTTAAA 
GGCCGAGTGG CCCTGGAAAT GGGGACACAT GAATTGCTCA TGACTGAACT AGTCTTCACA 
AACGCTCTGA CAGCGCTGCA GCCAGCTGAA ATTGCTGCTC TACTTTCTGC CCTAGTTTTT 
CAGCAAAAGA CTAATGTGGA ACCGCAGCTG ATCTCATCTC TGAAAAACGG ATGTAAAATC 
ATAGAAAAGG TTCAAAACAG CATTGCATTG GTGGAACAAC AAAACGCCGT TGACGTGGAA 
GAGCCATTAA ATTTCGGATT AGTGCAAGTT GTTTACGAAT GGGCAAGGTC AGAACCGTTC 
GCCAAAATAA TGGAACTGAC AGACGTCCAA GAAGGTATAA TCGTAAGATG TATACAGCAG 
TTAAATGAAA CCCTGAGATA TGTAAAAAAC GCTGCTATCA TTATTGGCGA CCCAGTGCTG 
AAGGAGAAGA TGGAAGAAGC ATCTTCTGCT ATAAGAAGAG ACATAGTTTT CGCTCAATCC 
TTCTATACCC AAGATTAA