LOC105682922 cDNA ORF clone, Athalia rosae(coleseed sawfly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105682922
Entrez Gene ID	105682922
Full Name	arginine--tRNA ligase, cytoplasmic
Gene Type	protein-coding
Organism	Athalia rosae(coleseed sawfly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012395167.2	XP_012250590.1	arginine--tRNA ligase, cytoplasmic

Pathways from BioSystems
Gene Ontology

The following LOC105682922 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105682922 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAt96135	XM_012395167.2 Latest version!	Athalia rosae arginine--tRNA ligase, cytoplasmic (LOC105682922), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105682922 ( XM_012395167.2 ) cDNA ORF clone, Athalia rosae(coleseed sawfly) -> XP_012250590.1 Athalia rosae arginine--tRNA ligase, cytoplasmic (LOC105682922), mRNA.

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CloneID	OAt96135
Clone ID Related Accession (Same CDS sequence)	XM_012395167.2
Accession Version	XM_012395167.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-16
Organism	Athalia rosae(coleseed sawfly)
Product	arginine--tRNA ligase, cytoplasmic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020311298.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 20, 2017 this sequence version replaced XM_012395167.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Athalia rosae Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTTAA ACGAACCTGA TAAAATGTCT ACAAGGGACG TGGAACTATT TCAGAAACGA 
GCTGCTGCCG CGGAGACGGA GATTAGAAAA TTAAAAGAAG CTCTAAAATC GTTTTATGAT 
GATGTAAAAG TGCATGGGAA TAATGAAACA ATGATAAGAC TACAAAATGA AAATGCTAAA 
CTCAAACATC GTCTCGCTAT TTTAAAGAGG GCTGTTGAAT GTGAAACATC TATCGCTTCT 
GAGAATGTCA TTCAGGACCA AAATCCAACT TTGAGTATTC AAGAACTGCT GCACAGCTTA 
TTCAGAGAAG CTATCGATGC TGCTTATCCA GAATTACCGG ATCTTCCGGT GATAATAGTT 
TCGAATGACA AGACTCCTCA ATTTGGAGAC TATCAATGTA ACAGCGCAAT GCTTATTTCA 
AACTTGATTA AGGCCAAAGG AACTAAAACT TCGCCACGTG ATGTTGCCAA TAAAATAGTT 
TCCAAAGTGA AATCAAACTC TTTAATCGAA AGAGTTGAAG TTGCAGGAGT AGGGTTTGTG 
AACATTTTTT TGAAACGTGA CTTTGCGGCT TCCATTCTTA CTACTATGTT GAAGAGGAGG 
CAGGTTTTAC CACCTTGTGT ACCCAAACAG AGAGTTCTTG TTGATTTTTC AAGCCCAAAT 
ATTGCCAAGG AGATGCATGT TGGACATCTA AGATCAACCA TCATTGGCGA TAGTATTAGT 
AGAGTTCTAG AATACTTGGG TCACGATGTA CTAAGAATCA ATCACATCGG TGATTGGGGT 
ACTCAGTTTG GAATGCTTGT TGCCCACCTA CAAGATCGTT TCCCTGATTA TCTCATCGTG 
TCTCCGCCAA TTTCAAGTCT TCAGGAATTT TATAAGGAAT CGAAAAAGAG ATTCGATGAA 
GATCCGTTGT TCAAAAAGCG TGCTTACGAG TGCGTTGTTA AGCTCCAGTC ATTTGATGCA 
GACGTCCTCA AAGCTTGGCA AATGATCTGC TCCGTTTCCA GAAAAGTGAA TCAGCAACTT 
TATGACCGCT TAGAGATTAA AATAACCGAA ATAGGAGAAT CCTTTTATCA GTCTCGTATG 
GTGGATATCG TTGAGGAGTT GGAGAGTCGT GGATTTTTGG AGGAAGATGA TGGCAGGAAA 
GTTATGTGGG GTACAAAGCC CGGCGTTGAT ATTCCATTAA CAATGGTCAA GTCTGACGGT 
GGATTCACTT ACGACACATC GGATATGGCA GCGATAAAGT ATCGGCTGGA AGAGCAGAAA 
GCAGATTGGG TCATATATGT GGTTGACGCA GGACAGGCAA CTCATTTCGA TACACTCAAA 
AAATGCGCCA AACGAGCTGG AATCATAACA AGCAAGCATA GAATAGACCA TGTTGAATTT 
GGTGTTGTAT TAGGAGAGGA TAAGAAAAAA TTCAAGACTA GATCTGGCGA AACAATTCAA 
CTCAGCAAGT TATTGGACGA AGGTTTGAAA CGAGCTTTAG AAAAACTGAA GGAGAAAGAG 
CGTGATAAGT TTCTGACCAA TGATGAATTA ATAGCTGCTC AGGAGTCCAT AGCATACGGC 
TGTATCAAGT ACTCGGATCT ATCGCATAAC CGAATCAAAA ATTATGTCTT TTCATTCGAT 
AGAATGCTCG ATGATAAAGG CAACACCGCT GTCTACCTAC TTTATGCTCT AACGAGAATA 
CGATCGATTG CTAGGGCTGC AAATGTCTCT CAAGAGCAAT TGCGAGCTGC TGCTGAGACA 
ACAGCAATCT CTTTGGAACA CGAAAAGGAA TTCAAGTTGG CCAAAGTTTT ACTCAAGTTT 
TCAGATGTCC TGTTAGAAAT AACAAAGGAT CTATGCCTAC ATCACTTGTG CGAATATTGT 
TATGAAATTT CGACTACATT TACAGAATTT TACGACAGTT GTTATTGCGT GGAAAAAAAT 
CAAGTCGGAG AAGTAGTGAA AATTAACATG GGCCGATTAA TGTTGGCCGA AGCAACTGCT 
ATGATAATGG AACAGGCGTT TAATCTCCTC GGTCTTAAGC CTGTAGAACG GATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012250590.1
CDS	100..2136
Translation	MHLNEPDKMSTRDVELFQKRAAAAETEIRKLKEALKSFYDDVKVHGNNETMIRLQNENAKLKHRLAILKRAVECETSIASENVIQDQNPTLSIQELLHSLFREAIDAAYPELPDLPVIIVSNDKTPQFGDYQCNSAMLISNLIKAKGTKTSPRDVANKIVSKVKSNSLIERVEVAGVGFVNIFLKRDFAASILTTMLKRRQVLPPCVPKQRVLVDFSSPNIAKEMHVGHLRSTIIGDSISRVLEYLGHDVLRINHIGDWGTQFGMLVAHLQDRFPDYLIVSPPISSLQEFYKESKKRFDEDPLFKKRAYECVVKLQSFDADVLKAWQMICSVSRKVNQQLYDRLEIKITEIGESFYQSRMVDIVEELESRGFLEEDDGRKVMWGTKPGVDIPLTMVKSDGGFTYDTSDMAAIKYRLEEQKADWVIYVVDAGQATHFDTLKKCAKRAGIITSKHRIDHVEFGVVLGEDKKKFKTRSGETIQLSKLLDEGLKRALEKLKEKERDKFLTNDELIAAQESIAYGCIKYSDLSHNRIKNYVFSFDRMLDDKGNTAVYLLYALTRIRSIARAANVSQEQLRAAAETTAISLEHEKEFKLAKVLLKFSDVLLEITKDLCLHHLCEYCYEISTTFTEFYDSCYCVEKNQVGEVVKINMGRLMLAEATAMIMEQAFNLLGLKPVERM

Target ORF information:

RefSeq Version	XM_012395167.2
Organism	Athalia rosae(coleseed sawfly)
Definition	Athalia rosae arginine--tRNA ligase, cytoplasmic (LOC105682922), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012395167.2

ORF Insert Sequence:

ATGCACTTAA ACGAACCTGA TAAAATGTCT ACAAGGGACG TGGAACTATT TCAGAAACGA 
GCTGCTGCCG CGGAGACGGA GATTAGAAAA TTAAAAGAAG CTCTAAAATC GTTTTATGAT 
GATGTAAAAG TGCATGGGAA TAATGAAACA ATGATAAGAC TACAAAATGA AAATGCTAAA 
CTCAAACATC GTCTCGCTAT TTTAAAGAGG GCTGTTGAAT GTGAAACATC TATCGCTTCT 
GAGAATGTCA TTCAGGACCA AAATCCAACT TTGAGTATTC AAGAACTGCT GCACAGCTTA 
TTCAGAGAAG CTATCGATGC TGCTTATCCA GAATTACCGG ATCTTCCGGT GATAATAGTT 
TCGAATGACA AGACTCCTCA ATTTGGAGAC TATCAATGTA ACAGCGCAAT GCTTATTTCA 
AACTTGATTA AGGCCAAAGG AACTAAAACT TCGCCACGTG ATGTTGCCAA TAAAATAGTT 
TCCAAAGTGA AATCAAACTC TTTAATCGAA AGAGTTGAAG TTGCAGGAGT AGGGTTTGTG 
AACATTTTTT TGAAACGTGA CTTTGCGGCT TCCATTCTTA CTACTATGTT GAAGAGGAGG 
CAGGTTTTAC CACCTTGTGT ACCCAAACAG AGAGTTCTTG TTGATTTTTC AAGCCCAAAT 
ATTGCCAAGG AGATGCATGT TGGACATCTA AGATCAACCA TCATTGGCGA TAGTATTAGT 
AGAGTTCTAG AATACTTGGG TCACGATGTA CTAAGAATCA ATCACATCGG TGATTGGGGT 
ACTCAGTTTG GAATGCTTGT TGCCCACCTA CAAGATCGTT TCCCTGATTA TCTCATCGTG 
TCTCCGCCAA TTTCAAGTCT TCAGGAATTT TATAAGGAAT CGAAAAAGAG ATTCGATGAA 
GATCCGTTGT TCAAAAAGCG TGCTTACGAG TGCGTTGTTA AGCTCCAGTC ATTTGATGCA 
GACGTCCTCA AAGCTTGGCA AATGATCTGC TCCGTTTCCA GAAAAGTGAA TCAGCAACTT 
TATGACCGCT TAGAGATTAA AATAACCGAA ATAGGAGAAT CCTTTTATCA GTCTCGTATG 
GTGGATATCG TTGAGGAGTT GGAGAGTCGT GGATTTTTGG AGGAAGATGA TGGCAGGAAA 
GTTATGTGGG GTACAAAGCC CGGCGTTGAT ATTCCATTAA CAATGGTCAA GTCTGACGGT 
GGATTCACTT ACGACACATC GGATATGGCA GCGATAAAGT ATCGGCTGGA AGAGCAGAAA 
GCAGATTGGG TCATATATGT GGTTGACGCA GGACAGGCAA CTCATTTCGA TACACTCAAA 
AAATGCGCCA AACGAGCTGG AATCATAACA AGCAAGCATA GAATAGACCA TGTTGAATTT 
GGTGTTGTAT TAGGAGAGGA TAAGAAAAAA TTCAAGACTA GATCTGGCGA AACAATTCAA 
CTCAGCAAGT TATTGGACGA AGGTTTGAAA CGAGCTTTAG AAAAACTGAA GGAGAAAGAG 
CGTGATAAGT TTCTGACCAA TGATGAATTA ATAGCTGCTC AGGAGTCCAT AGCATACGGC 
TGTATCAAGT ACTCGGATCT ATCGCATAAC CGAATCAAAA ATTATGTCTT TTCATTCGAT 
AGAATGCTCG ATGATAAAGG CAACACCGCT GTCTACCTAC TTTATGCTCT AACGAGAATA 
CGATCGATTG CTAGGGCTGC AAATGTCTCT CAAGAGCAAT TGCGAGCTGC TGCTGAGACA 
ACAGCAATCT CTTTGGAACA CGAAAAGGAA TTCAAGTTGG CCAAAGTTTT ACTCAAGTTT 
TCAGATGTCC TGTTAGAAAT AACAAAGGAT CTATGCCTAC ATCACTTGTG CGAATATTGT 
TATGAAATTT CGACTACATT TACAGAATTT TACGACAGTT GTTATTGCGT GGAAAAAAAT 
CAAGTCGGAG AAGTAGTGAA AATTAACATG GGCCGATTAA TGTTGGCCGA AGCAACTGCT 
ATGATAATGG AACAGGCGTT TAATCTCCTC GGTCTTAAGC CTGTAGAACG GATGTAA