THEMIS cDNA ORF clone, Aquila chrysaetos canadensis

Gene
Clones
gRNAs

Summary

Gene Symbol	THEMIS
Entrez Gene ID	105414308
Full Name	thymocyte selection associated
Gene Type	protein-coding
Organism	Aquila chrysaetos canadensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_011599702.1	XP_011598004.1	protein THEMIS

Pathways from BioSystems
Gene Ontology

The following THEMIS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THEMIS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAq11202	XM_011599702.1 Latest version!	Aquila chrysaetos canadensis thymocyte selection associated (THEMIS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THEMIS ( XM_011599702.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011598004.1 Aquila chrysaetos canadensis thymocyte selection associated (THEMIS), mRNA.

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CloneID	OAq11202
Clone ID Related Accession (Same CDS sequence)	XM_011599702.1
Accession Version	XM_011599702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	protein THEMIS
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950873.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTA CCCTGGAGAA GTTCATTCAT TCTCTGGACC CGAGAGCCCT TCCTAGGGTC 
CTCCAAATCC AGTCAGGCAT CTATTTCCAA GGCTCCATCT ATGAAATGTT TGGAAATGAA 
TGCTGCCTGT CAACAGGAGA AGTCATTAAA GTTATTGGCT TCAAAATTAA TAAGCTCATA 
GCAAGTATCT GTGAGAATAA TGAAGTCAGC CGGTTTTCAG CCAAAGTGGA ATTACCACTA 
AATTTTCCTG GTCTGTATAA GGTTGTGGCA GATAAAACTC CATATGTCAG CATTGAAGAA 
ATTACAAGAA AAGTCTCTAT TGGGCCAACT AGATTTGGCC ATCCGTGTTT CTACAGCCCT 
GAGGATATAA AATTGGCAAA CATCACCATC AAACACGGTG AGCAGATCAC CTTCAACTCA 
GTGGAAGAGG TCAATGGAAC GATGGCTGTG AACTGTGGTG TGGTCAGAAA TAACCAGTCT 
CATTCCTTTA CATTGCCCTT TTCACAAGAA GGAGAATTCT ACGAGTGCGA AGATGACCAG 
ATATACACCC TAAAAGAGAT TGCAGAATGG AAAATCCCTA AGTGCAGAAA CCGGATTGTC 
AAACTTTCCA ATACTTTACA TACGTGGGAC TCCTCTAATC CACTCCCTGA GAATTTTGAT 
GGCTGCTTGA TTCTGACGCC TGTCTATGAA GTGCAGGCAG TAATGAAATT TCGAAAGGAC 
ATAGTTCATA TCCTCTCAGA TCTAGATGTC GAAGTCAAGG ATATAACAGA CTGTTATGAT 
ATTAGCTCTT TCCTTCAGCC ACTCTCTTTG GAAGATGTAT TTGAGAGAAC AAGCAAGGAA 
TTTCCCATGG TTGCTGAGAT AATGGAAGGG CCTTCAGGAA GCCAAAAACC TTATAACTTA 
TTGTATACAG GAAAAGAGAT CATCATCTAC AAAAAGTACC AGGCAACACG AGTCCTAGCC 
TCTGAGATCA GAAGTGATTC CCCTAAAAGA CATTTTTTAA TCCCTATGAG CTACAAAGGA 
AAGTTCAAGA GACGACCTCG GGAGTTTCCA ACTGCCTATG ACTTAGAGAT AGCAAGAAGT 
GAAAAGGAAC AACTTCATGT TGTAGCAACT AAAGCCTTTG ATTCCCCTCA TAAAGAGCTT 
TTTTCTGTTT CGGTTGGAGA TCAATTTCTA GTTCAGCAAT GCCAGACTAG TGAAGTTCTG 
TATGAGGGAA GAAAGAAACT CATAGATGTT TTAGCTTGTG AACAAATACT AAGTGATACA 
TATAAGAAAG TGCTCCTCCC TATGTATATG GAAGGTGGCT TTGTGGAAGT GATTCATGAC 
AAGAAACAGT ACCAGCTTTC TGAGATTTGC AAAGAATTCC GTTTGCCTTT TAATGTCAAA 
GTGTCCGTGA GGGACCTTTC CATTGAGGAA GATGTCTTGG CTGCTGTGCC TGGTCTGCAG 
TTTGAAGAAG AAATCACAGA CTCCTATTTG CTGATCAGTA GTGCCAGCAG TCCAGTGGAG 
AGCTGGGAGA TTCCTGTGTA TCGCCTAAAC ATGTCGGTCC ATTTGCTCAG CAAAGATGGC 
CAGACAGTTG TACCACCTGT GACTAAGACA ACAGTGGAAG AGATCAGCGA AGAGCAATAC 
TATATGGTGC GAAGATATGA AAACCAAACC CTTCATCCAC CACCAAGGCC TCCCAAAAAG 
CCAACACTTG CACCAAAACC TGTTTTTGCA GTGCCTAAGC AAGATGTGTC TGATGTACTA 
CAGGCTCCAA AGAATTTCTG TGTAGACACC ACCAAGAAAC TGTTCTCAAA TCAAGCTGCC 
AGTGTTTCGG AATTACAAGT TAGTTGTCAA AATGATTTGG TGCATCGTGA GAATAAGAAG 
GACATGACTG GGAAAGCTAC ATTAGTGGAA ACCAGTGTGA TTAAAGGTCT AACAAATAAG 
GCACTTGGAA AGTGTGAGGC AGAAAATGAA GAGGAAGAAG AGCACAACTA TGACTATGTG 
GATGAAAACA TAATCGAAAA CAAAAGAAAA ATAAGTCAGG ACACCATCGT TAGAAATAAG 
CATGCGGTCT CTGAAGGAAG ACGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011598004.1
CDS	1..2067
Translation	MASTLEKFIHSLDPRALPRVLQIQSGIYFQGSIYEMFGNECCLSTGEVIKVIGFKINKLIASICENNEVSRFSAKVELPLNFPGLYKVVADKTPYVSIEEITRKVSIGPTRFGHPCFYSPEDIKLANITIKHGEQITFNSVEEVNGTMAVNCGVVRNNQSHSFTLPFSQEGEFYECEDDQIYTLKEIAEWKIPKCRNRIVKLSNTLHTWDSSNPLPENFDGCLILTPVYEVQAVMKFRKDIVHILSDLDVEVKDITDCYDISSFLQPLSLEDVFERTSKEFPMVAEIMEGPSGSQKPYNLLYTGKEIIIYKKYQATRVLASEIRSDSPKRHFLIPMSYKGKFKRRPREFPTAYDLEIARSEKEQLHVVATKAFDSPHKELFSVSVGDQFLVQQCQTSEVLYEGRKKLIDVLACEQILSDTYKKVLLPMYMEGGFVEVIHDKKQYQLSEICKEFRLPFNVKVSVRDLSIEEDVLAAVPGLQFEEEITDSYLLISSASSPVESWEIPVYRLNMSVHLLSKDGQTVVPPVTKTTVEEISEEQYYMVRRYENQTLHPPPRPPKKPTLAPKPVFAVPKQDVSDVLQAPKNFCVDTTKKLFSNQAASVSELQVSCQNDLVHRENKKDMTGKATLVETSVIKGLTNKALGKCEAENEEEEEHNYDYVDENIIENKRKISQDTIVRNKHAVSEGRR

Target ORF information:

RefSeq Version	XM_011599702.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis thymocyte selection associated (THEMIS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011599702.1

ORF Insert Sequence:

ATGGCATCTA CCCTGGAGAA GTTCATTCAT TCTCTGGACC CGAGAGCCCT TCCTAGGGTC 
CTCCAAATCC AGTCAGGCAT CTATTTCCAA GGCTCCATCT ATGAAATGTT TGGAAATGAA 
TGCTGCCTGT CAACAGGAGA AGTCATTAAA GTTATTGGCT TCAAAATTAA TAAGCTCATA 
GCAAGTATCT GTGAGAATAA TGAAGTCAGC CGGTTTTCAG CCAAAGTGGA ATTACCACTA 
AATTTTCCTG GTCTGTATAA GGTTGTGGCA GATAAAACTC CATATGTCAG CATTGAAGAA 
ATTACAAGAA AAGTCTCTAT TGGGCCAACT AGATTTGGCC ATCCGTGTTT CTACAGCCCT 
GAGGATATAA AATTGGCAAA CATCACCATC AAACACGGTG AGCAGATCAC CTTCAACTCA 
GTGGAAGAGG TCAATGGAAC GATGGCTGTG AACTGTGGTG TGGTCAGAAA TAACCAGTCT 
CATTCCTTTA CATTGCCCTT TTCACAAGAA GGAGAATTCT ACGAGTGCGA AGATGACCAG 
ATATACACCC TAAAAGAGAT TGCAGAATGG AAAATCCCTA AGTGCAGAAA CCGGATTGTC 
AAACTTTCCA ATACTTTACA TACGTGGGAC TCCTCTAATC CACTCCCTGA GAATTTTGAT 
GGCTGCTTGA TTCTGACGCC TGTCTATGAA GTGCAGGCAG TAATGAAATT TCGAAAGGAC 
ATAGTTCATA TCCTCTCAGA TCTAGATGTC GAAGTCAAGG ATATAACAGA CTGTTATGAT 
ATTAGCTCTT TCCTTCAGCC ACTCTCTTTG GAAGATGTAT TTGAGAGAAC AAGCAAGGAA 
TTTCCCATGG TTGCTGAGAT AATGGAAGGG CCTTCAGGAA GCCAAAAACC TTATAACTTA 
TTGTATACAG GAAAAGAGAT CATCATCTAC AAAAAGTACC AGGCAACACG AGTCCTAGCC 
TCTGAGATCA GAAGTGATTC CCCTAAAAGA CATTTTTTAA TCCCTATGAG CTACAAAGGA 
AAGTTCAAGA GACGACCTCG GGAGTTTCCA ACTGCCTATG ACTTAGAGAT AGCAAGAAGT 
GAAAAGGAAC AACTTCATGT TGTAGCAACT AAAGCCTTTG ATTCCCCTCA TAAAGAGCTT 
TTTTCTGTTT CGGTTGGAGA TCAATTTCTA GTTCAGCAAT GCCAGACTAG TGAAGTTCTG 
TATGAGGGAA GAAAGAAACT CATAGATGTT TTAGCTTGTG AACAAATACT AAGTGATACA 
TATAAGAAAG TGCTCCTCCC TATGTATATG GAAGGTGGCT TTGTGGAAGT GATTCATGAC 
AAGAAACAGT ACCAGCTTTC TGAGATTTGC AAAGAATTCC GTTTGCCTTT TAATGTCAAA 
GTGTCCGTGA GGGACCTTTC CATTGAGGAA GATGTCTTGG CTGCTGTGCC TGGTCTGCAG 
TTTGAAGAAG AAATCACAGA CTCCTATTTG CTGATCAGTA GTGCCAGCAG TCCAGTGGAG 
AGCTGGGAGA TTCCTGTGTA TCGCCTAAAC ATGTCGGTCC ATTTGCTCAG CAAAGATGGC 
CAGACAGTTG TACCACCTGT GACTAAGACA ACAGTGGAAG AGATCAGCGA AGAGCAATAC 
TATATGGTGC GAAGATATGA AAACCAAACC CTTCATCCAC CACCAAGGCC TCCCAAAAAG 
CCAACACTTG CACCAAAACC TGTTTTTGCA GTGCCTAAGC AAGATGTGTC TGATGTACTA 
CAGGCTCCAA AGAATTTCTG TGTAGACACC ACCAAGAAAC TGTTCTCAAA TCAAGCTGCC 
AGTGTTTCGG AATTACAAGT TAGTTGTCAA AATGATTTGG TGCATCGTGA GAATAAGAAG 
GACATGACTG GGAAAGCTAC ATTAGTGGAA ACCAGTGTGA TTAAAGGTCT AACAAATAAG 
GCACTTGGAA AGTGTGAGGC AGAAAATGAA GAGGAAGAAG AGCACAACTA TGACTATGTG 
GATGAAAACA TAATCGAAAA CAAAAGAAAA ATAAGTCAGG ACACCATCGT TAGAAATAAG 
CATGCGGTCT CTGAAGGAAG ACGGTAA