POLR3B cDNA ORF clone, Aquila chrysaetos canadensis

Gene
Clones
gRNAs

Summary

Gene Symbol	POLR3B
Entrez Gene ID	105411234
Full Name	RNA polymerase III subunit B
Gene Type	protein-coding
Organism	Aquila chrysaetos canadensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_011593839.1	XP_011592141.1	DNA-directed RNA polymerase III subunit RPC2 isoform X2
XM_011593838.1	XP_011592140.1	DNA-directed RNA polymerase III subunit RPC2 isoform X1

Pathways from BioSystems
Gene Ontology

The following POLR3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAq28637	XM_011593839.1 Latest version!	Aquila chrysaetos canadensis polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAq28636	XM_011593838.1 Latest version!	Aquila chrysaetos canadensis polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLR3B ( XM_011593839.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011592141.1 Aquila chrysaetos canadensis polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OAq28637
Clone ID Related Accession (Same CDS sequence)	XM_011593839.1
Accession Version	XM_011593839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	DNA-directed RNA polymerase III subunit RPC2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGC TGGCCACGGA GCTGGGGGCT CTGACTCCGG AGCAAGCGGC GGCTCCTGTG 
AACACGGTGG AGGAAAAATG GAGACTTCTT CCAGCATTTC TAAAGGTGAA AGGACTCGTG 
AAGCAGCACA TAGACTCATT CAACTATTTC ATCAATGTAG AGATAAAGAA AATCATGAAG 
GCCAATGAAA AAGTTACAAG TGATGCTGAC CCAATGTGGT ACTTGAAGTA CCTCAATATC 
TATGTGGGTA CTCCAGATGT GGAAGAAAGC TTCAATGTGA CACGACCTGT ATCGCCTCAT 
GAGTGCCGCT TGAGAGACAT GACTTATTCT GCCCCGATTA CAGTGGACAT TGAATATACA 
CGAGGTAGCC AGAGGATCAT ACGCAATGCA TTACCCATTG GCAGAATGCC TATAATGCTG 
CGTAGCTCCA ATTGTGTTCT TACTGGGAAA ACTCCAGCAG AATTTGCCAA ACTTAATGAA 
TGTCCTTTAG ATCCAGGTGG CTATTTCATT GTTAAAGGTG TAGAAAAAGT CATCCTTATT 
CAAGAGCAGT TATCCAAAAA CAGAATCATT GTGGAAGCTG ATAGAAAAGG CACAGTTGGC 
GCTTCTGTTA CCAGTTCCAC TCATGAGAAG AAGAGCAGAA CAAATATGGT TGTGAAACAA 
GGAAGATTTT ACCTCAGGCA CAACACTCTG TCAGAAGATA TTCCCATTGC TATCATATTT 
AAGGCCATGG GTGTTGAGAG TGACCAAGAG ATTGTACAAA TGATTGGCAC AGAGGAACAT 
GTGATGGCTG CATTTGCTCC TAGTCTGGAA GAATGCCAAA AAGCTCAGAT TTTTACACAG 
ATGCAGGCAT TGAAGTACAT TGGAAACAAA GTACGAAGGC AAAGGATGTG GGGAGGCCCA 
AAGAAAACAA AGATGGAAGA AGCACGAGAG CTGCTAGCAT CAACTATTCT GACCCACGTT 
CTTGTGAAGG AATTCAATTT TCGTGCCAAG TGCATATACA CAGCAGTGAT GGTGCGCAGA 
GTAATCTTGG CTCAAGGAGA AAATAAAGTA GATGACAGAG ACTACTATGG AAATAAACGT 
TTGGAGTTGG CAGGGCAGCT TCTGTCTCTT CTATTTGAAG ATTTGTTCAA AAAATTTAAC 
TCTGAACTGA AAAAGATTGC TGACCAGGTA ATCCCCAAGC AACGTGCAGC TCAGTTTGAT 
GTAGTGAAGC ACATGCGTCA AGACCAAATC ACCAATGGCA TGGTCAATGC TATCTCCACA 
GGAAATTGGT CTCTGAAGAG ATTCAAAATG GACCGCCAAG GCGTGACACA AGTATTGTCC 
CGCTTGTCTT ACATATCTGC TCTGGGAATG ATGACTAGAA TCTCTTCTCA GTTTGAAAAG 
ACAAGGAAAG TGAGTGGTCC TCGGTCACTA CAACCATCTC AGTGGGGGAT GCTCTGCCCA 
TCTGACACTC CTGAAGGAGA GGCTTGTGGT TTGGTTAAAA ACCTTGCCCT CATGACTCAC 
ATTACTACAG ATATGGAAGA TGGCCCAATT ATTAAATTAG CCAGTAATCT TGGAGTTGAA 
GATGTCAACT TGCTCTGTGG TGAAGAACTT TCATACCCAA ATGTGTTCCT TGTTTTCCTT 
AATGGTAACA TCCTCGGTGT CATACGTGAT CATCAGAAAT TAGTGAACAC GTTTCGGATA 
ATGCGTCGAG CGGGTTATAT CAATGAATTT GTTTCCATCT CTACAAATCT TTCTGACAGA 
TGTGTCTACA TTTCCTCTGA TGGAGGGAGA TTGTGCAGAC CCTACATAAT TGTCAAGAAG 
CAAAAACCTG CGGTTACAAA CAAGCATATG GAAGAACTGG CTCAGGGATA CAGGAATTTT 
GAAGACTTCT TGCACGAAGG CCTTGTTGAG TATTTGGATG TGAATGAAGA AAATGACTGT 
AACATTGCAC TATATGAACA CACAATTAAT AAAGATACAA CGCATTTGGA GATTGAGCCT 
TTCACGCTTC TTGGTGTCTG TGCTGGGCTG ATTCCGTATC CTCACCACAA CCAGTCCCCA 
AGAAACACTT ATCAGTGTGC CATGGGGAAA CAAGCAATGG GTACTATTGG ATACAATCAA 
AGAAACAGGA TAGATACTCT TATGTATCTT CTAGCCTATC CACAAAAGCC AATGGTAAAA 
ACAAAAACAA TTGAACTGAT AGATTTTGAG AAACTGCCTG CTGGACAGAA TGCCACAGTT 
GCTGTGATGA GCTACAGTGG TTACGATATT GAAGATGCTC TTGTCTTAAA CAAGGCCTCT 
TTGGATAGAG GTTTTGGAAG GTGTCTTGTA TACAAGAACG CCAAGTGTAC ACTGAAGCGT 
TACACAAACC AGACATTTGA TAAAGTTATG GGTCCGATGT TGGATGCAGC TACCCGTAAA 
CCAATCTGGC GCCATGAAAT TTTAGATGCT GATGGTATCT GCTCTCCAGG TGAAAAAGTA 
GAAAATAAGC AAGTCCTTGT GAACAAATCT ATGCCAACTG TGACCCAGAC ACCTCTGGAA 
GGAAGTAGTG TGCCTCAGCA GCCACAGTAC AAAGATGTGC CAGTAACCTA CAAAGGTGCG 
ACTGATTCTT ATATTGAAAA AGTAATGATT TCATCAAATG CAGAGGATGC TTTCTTGATA 
AAAATGTTGC TGAGGCAAAC TAGACGTCCA GAAATTGGTG ATAAATTTAG CAGTCGTCAT 
GGGCAGAAAG CACATACACT CCTTTCCTGT TACTGGGCAA ATGTGATACA TCTTAATAAC 
TACTGGAACA AAAAAATTCT TACTCCATTG CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011592141.1
CDS	27..2822
Translation	MEALATELGALTPEQAAAPVNTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDADPMWYLKYLNIYVGTPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGTVGASVTSSTHEKKSRTNMVVKQGRFYLRHNTLSEDIPIAIIFKAMGVESDQEIVQMIGTEEHVMAAFAPSLEECQKAQIFTQMQALKYIGNKVRRQRMWGGPKKTKMEEARELLASTILTHVLVKEFNFRAKCIYTAVMVRRVILAQGENKVDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSELKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAISTGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMEDGPIIKLASNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHQKLVNTFRIMRRAGYINEFVSISTNLSDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHEGLVEYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIELIDFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQTPLEGSSVPQQPQYKDVPVTYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKAHTLLSCYWANVIHLNNYWNKKILTPLL

Target ORF information:

RefSeq Version	XM_011593839.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011593839.1

ORF Insert Sequence:

ATGGAGGCGC TGGCCACGGA GCTGGGGGCT CTGACTCCGG AGCAAGCGGC GGCTCCTGTG 
AACACGGTGG AGGAAAAATG GAGACTTCTT CCAGCATTTC TAAAGGTGAA AGGACTCGTG 
AAGCAGCACA TAGACTCATT CAACTATTTC ATCAATGTAG AGATAAAGAA AATCATGAAG 
GCCAATGAAA AAGTTACAAG TGATGCTGAC CCAATGTGGT ACTTGAAGTA CCTCAATATC 
TATGTGGGTA CTCCAGATGT GGAAGAAAGC TTCAATGTGA CACGACCTGT ATCGCCTCAT 
GAGTGCCGCT TGAGAGACAT GACTTATTCT GCCCCGATTA CAGTGGACAT TGAATATACA 
CGAGGTAGCC AGAGGATCAT ACGCAATGCA TTACCCATTG GCAGAATGCC TATAATGCTG 
CGTAGCTCCA ATTGTGTTCT TACTGGGAAA ACTCCAGCAG AATTTGCCAA ACTTAATGAA 
TGTCCTTTAG ATCCAGGTGG CTATTTCATT GTTAAAGGTG TAGAAAAAGT CATCCTTATT 
CAAGAGCAGT TATCCAAAAA CAGAATCATT GTGGAAGCTG ATAGAAAAGG CACAGTTGGC 
GCTTCTGTTA CCAGTTCCAC TCATGAGAAG AAGAGCAGAA CAAATATGGT TGTGAAACAA 
GGAAGATTTT ACCTCAGGCA CAACACTCTG TCAGAAGATA TTCCCATTGC TATCATATTT 
AAGGCCATGG GTGTTGAGAG TGACCAAGAG ATTGTACAAA TGATTGGCAC AGAGGAACAT 
GTGATGGCTG CATTTGCTCC TAGTCTGGAA GAATGCCAAA AAGCTCAGAT TTTTACACAG 
ATGCAGGCAT TGAAGTACAT TGGAAACAAA GTACGAAGGC AAAGGATGTG GGGAGGCCCA 
AAGAAAACAA AGATGGAAGA AGCACGAGAG CTGCTAGCAT CAACTATTCT GACCCACGTT 
CTTGTGAAGG AATTCAATTT TCGTGCCAAG TGCATATACA CAGCAGTGAT GGTGCGCAGA 
GTAATCTTGG CTCAAGGAGA AAATAAAGTA GATGACAGAG ACTACTATGG AAATAAACGT 
TTGGAGTTGG CAGGGCAGCT TCTGTCTCTT CTATTTGAAG ATTTGTTCAA AAAATTTAAC 
TCTGAACTGA AAAAGATTGC TGACCAGGTA ATCCCCAAGC AACGTGCAGC TCAGTTTGAT 
GTAGTGAAGC ACATGCGTCA AGACCAAATC ACCAATGGCA TGGTCAATGC TATCTCCACA 
GGAAATTGGT CTCTGAAGAG ATTCAAAATG GACCGCCAAG GCGTGACACA AGTATTGTCC 
CGCTTGTCTT ACATATCTGC TCTGGGAATG ATGACTAGAA TCTCTTCTCA GTTTGAAAAG 
ACAAGGAAAG TGAGTGGTCC TCGGTCACTA CAACCATCTC AGTGGGGGAT GCTCTGCCCA 
TCTGACACTC CTGAAGGAGA GGCTTGTGGT TTGGTTAAAA ACCTTGCCCT CATGACTCAC 
ATTACTACAG ATATGGAAGA TGGCCCAATT ATTAAATTAG CCAGTAATCT TGGAGTTGAA 
GATGTCAACT TGCTCTGTGG TGAAGAACTT TCATACCCAA ATGTGTTCCT TGTTTTCCTT 
AATGGTAACA TCCTCGGTGT CATACGTGAT CATCAGAAAT TAGTGAACAC GTTTCGGATA 
ATGCGTCGAG CGGGTTATAT CAATGAATTT GTTTCCATCT CTACAAATCT TTCTGACAGA 
TGTGTCTACA TTTCCTCTGA TGGAGGGAGA TTGTGCAGAC CCTACATAAT TGTCAAGAAG 
CAAAAACCTG CGGTTACAAA CAAGCATATG GAAGAACTGG CTCAGGGATA CAGGAATTTT 
GAAGACTTCT TGCACGAAGG CCTTGTTGAG TATTTGGATG TGAATGAAGA AAATGACTGT 
AACATTGCAC TATATGAACA CACAATTAAT AAAGATACAA CGCATTTGGA GATTGAGCCT 
TTCACGCTTC TTGGTGTCTG TGCTGGGCTG ATTCCGTATC CTCACCACAA CCAGTCCCCA 
AGAAACACTT ATCAGTGTGC CATGGGGAAA CAAGCAATGG GTACTATTGG ATACAATCAA 
AGAAACAGGA TAGATACTCT TATGTATCTT CTAGCCTATC CACAAAAGCC AATGGTAAAA 
ACAAAAACAA TTGAACTGAT AGATTTTGAG AAACTGCCTG CTGGACAGAA TGCCACAGTT 
GCTGTGATGA GCTACAGTGG TTACGATATT GAAGATGCTC TTGTCTTAAA CAAGGCCTCT 
TTGGATAGAG GTTTTGGAAG GTGTCTTGTA TACAAGAACG CCAAGTGTAC ACTGAAGCGT 
TACACAAACC AGACATTTGA TAAAGTTATG GGTCCGATGT TGGATGCAGC TACCCGTAAA 
CCAATCTGGC GCCATGAAAT TTTAGATGCT GATGGTATCT GCTCTCCAGG TGAAAAAGTA 
GAAAATAAGC AAGTCCTTGT GAACAAATCT ATGCCAACTG TGACCCAGAC ACCTCTGGAA 
GGAAGTAGTG TGCCTCAGCA GCCACAGTAC AAAGATGTGC CAGTAACCTA CAAAGGTGCG 
ACTGATTCTT ATATTGAAAA AGTAATGATT TCATCAAATG CAGAGGATGC TTTCTTGATA 
AAAATGTTGC TGAGGCAAAC TAGACGTCCA GAAATTGGTG ATAAATTTAG CAGTCGTCAT 
GGGCAGAAAG CACATACACT CCTTTCCTGT TACTGGGCAA ATGTGATACA TCTTAATAAC 
TACTGGAACA AAAAAATTCT TACTCCATTG CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR3B ( XM_011593838.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011592140.1 Aquila chrysaetos canadensis polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAq28636
Clone ID Related Accession (Same CDS sequence)	XM_011593838.1
Accession Version	XM_011593838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	DNA-directed RNA polymerase III subunit RPC2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950980.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGC TGGCCACGGA GCTGGGGGCT CTGACTCCGG AGCAAGCGGC GGCTCCTGTG 
AACACGGTGG AGGAAAAATG GAGACTTCTT CCAGCATTTC TAAAGGTGAA AGGACTCGTG 
AAGCAGCACA TAGACTCATT CAACTATTTC ATCAATGTAG AGATAAAGAA AATCATGAAG 
GCCAATGAAA AAGTTACAAG TGATGCTGAC CCAATGTGGT ACTTGAAGTA CCTCAATATC 
TATGTGGGTA CTCCAGATGT GGAAGAAAGC TTCAATGTGA CACGACCTGT ATCGCCTCAT 
GAGTGCCGCT TGAGAGACAT GACTTATTCT GCCCCGATTA CAGTGGACAT TGAATATACA 
CGAGGTAGCC AGAGGATCAT ACGCAATGCA TTACCCATTG GCAGAATGCC TATAATGCTG 
CGTAGCTCCA ATTGTGTTCT TACTGGGAAA ACTCCAGCAG AATTTGCCAA ACTTAATGAA 
TGTCCTTTAG ATCCAGGTGG CTATTTCATT GTTAAAGGTG TAGAAAAAGT CATCCTTATT 
CAAGAGCAGT TATCCAAAAA CAGAATCATT GTGGAAGCTG ATAGAAAAGG CACAGTTGGC 
GCTTCTGTTA CCAGTTCCAC TCATGAGAAG AAGAGCAGAA CAAATATGGT TGTGAAACAA 
GGAAGATTTT ACCTCAGGCA CAACACTCTG TCAGAAGATA TTCCCATTGC TATCATATTT 
AAGGCCATGG GTGTTGAGAG TGACCAAGAG ATTGTACAAA TGATTGGCAC AGAGGAACAT 
GTGATGGCTG CATTTGCTCC TAGTCTGGAA GAATGCCAAA AAGCTCAGAT TTTTACACAG 
ATGCAGGCAT TGAAGTACAT TGGAAACAAA GTACGAAGGC AAAGGATGTG GGGAGGCCCA 
AAGAAAACAA AGATGGAAGA AGCACGAGAG CTGCTAGCAT CAACTATTCT GACCCACGTT 
CTTGTGAAGG AATTCAATTT TCGTGCCAAG TGCATATACA CAGCAGTGAT GGTGCGCAGA 
GTAATCTTGG CTCAAGGAGA AAATAAAGTA GATGACAGAG ACTACTATGG AAATAAACGT 
TTGGAGTTGG CAGGGCAGCT TCTGTCTCTT CTATTTGAAG ATTTGTTCAA AAAATTTAAC 
TCTGAACTGA AAAAGATTGC TGACCAGGTA ATCCCCAAGC AACGTGCAGC TCAGTTTGAT 
GTAGTGAAGC ACATGCGTCA AGACCAAATC ACCAATGGCA TGGTCAATGC TATCTCCACA 
GGAAATTGGT CTCTGAAGAG ATTCAAAATG GACCGCCAAG GCGTGACACA AGTATTGTCC 
CGCTTGTCTT ACATATCTGC TCTGGGAATG ATGACTAGAA TCTCTTCTCA GTTTGAAAAG 
ACAAGGAAAG TGAGTGGTCC TCGGTCACTA CAACCATCTC AGTGGGGGAT GCTCTGCCCA 
TCTGACACTC CTGAAGGAGA GGCTTGTGGT TTGGTTAAAA ACCTTGCCCT CATGACTCAC 
ATTACTACAG ATATGGAAGA TGGCCCAATT ATTAAATTAG CCAGTAATCT TGGAGTTGAA 
GATGTCAACT TGCTCTGTGG TGAAGAACTT TCATACCCAA ATGTGTTCCT TGTTTTCCTT 
AATGGTAACA TCCTCGGTGT CATACGTGAT CATCAGAAAT TAGTGAACAC GTTTCGGATA 
ATGCGTCGAG CGGGTTATAT CAATGAATTT GTTTCCATCT CTACAAATCT TTCTGACAGA 
TGTGTCTACA TTTCCTCTGA TGGAGGGAGA TTGTGCAGAC CCTACATAAT TGTCAAGAAG 
CAAAAACCTG CGGTTACAAA CAAGCATATG GAAGAACTGG CTCAGGGATA CAGGAATTTT 
GAAGACTTCT TGCACGAAGG CCTTGTTGAG TATTTGGATG TGAATGAAGA AAATGACTGT 
AACATTGCAC TATATGAACA CACAATTAAT AAAGATACAA CGCATTTGGA GATTGAGCCT 
TTCACGCTTC TTGGTGTCTG TGCTGGGCTG ATTCCGTATC CTCACCACAA CCAGTCCCCA 
AGAAACACTT ATCAGTGTGC CATGGGGAAA CAAGCAATGG GTACTATTGG ATACAATCAA 
AGAAACAGGA TAGATACTCT TATGTATCTT CTAGCCTATC CACAAAAGCC AATGGTAAAA 
ACAAAAACAA TTGAACTGAT AGATTTTGAG AAACTGCCTG CTGGACAGAA TGCCACAGTT 
GCTGTGATGA GCTACAGTGG TTACGATATT GAAGATGCTC TTGTCTTAAA CAAGGCCTCT 
TTGGATAGAG GTTTTGGAAG GTGTCTTGTA TACAAGAACG CCAAGTGTAC ACTGAAGCGT 
TACACAAACC AGACATTTGA TAAAGTTATG GGTCCGATGT TGGATGCAGC TACCCGTAAA 
CCAATCTGGC GCCATGAAAT TTTAGATGCT GATGGTATCT GCTCTCCAGG TGAAAAAGTA 
GAAAATAAGC AAGTCCTTGT GAACAAATCT ATGCCAACTG TGACCCAGAC ACCTCTGGAA 
GGAAGTAGTG TGCCTCAGCA GCCACAGTAC AAAGATGTGC CAGTAACCTA CAAAGGTGCG 
ACTGATTCTT ATATTGAAAA AGTAATGATT TCATCAAATG CAGAGGATGC TTTCTTGATA 
AAAATGTTGC TGAGGCAAAC TAGACGTCCA GAAATTGGTG ATAAATTTAG CAGTCGTCAT 
GGGCAGAAAG GGGTGTGTGG ACTGATTGTT CCTCAGGAAG ACATGCCATT TTGCGATACA 
GGAATCTGTC CAGATATCAT AATGAACCCT CATGGCTTCC CATCGCGTAT GACGGTAGGA 
AAGTTAATTG AACTCCTGGC TGGAAAGGCT GGGGTCCTTG ACGGCAGGTT TCACTATGGA 
ACAGCTTTTG GAGGCAGTAA AGTTAAGGAT GTGTGTGAAG ATCTTATTCG CCATGGTTAT 
AACTACCTAG GGAAGGACTA TGTAACATCT GGTATCACTG GGGAACCTTT GGAAGCATAT 
ATCTATTTTG GCCCTGTGTA TTACCAGAAA CTAAAGCACA TGGTGTTGGA TAAAATGCAT 
GCAAGAGCTC GAGGACCCAG AGCAGTGCTC ACCAGGCAAC CCACTGAAGG TCGATCCCGT 
GATGGTGGTT TACGTCTTGG AGAGATGGAA CGGGATTGTT TGATAGGTTA TGGAGCCAGC 
ATGCTTTTAC TGGAGAGACT GATGATTTCA AGTGATGCCT TTGAAGTGGA TGTTTGTGGG 
CAGTGTGGCT TGCTGGGGTA CTCTGGCTGG TGCCACTACT GCAAATCATC ATGTCATGTG 
TCTTCTCTGC GCATACCTTA TGCCTGTAAA CTCTTATTCC AAGAACTGCA GTCAATGAAC 
ATCATACCTA GGTTAAAACT AGCTAAGTAC AATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011592140.1
CDS	27..3425
Translation	MEALATELGALTPEQAAAPVNTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDADPMWYLKYLNIYVGTPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGTVGASVTSSTHEKKSRTNMVVKQGRFYLRHNTLSEDIPIAIIFKAMGVESDQEIVQMIGTEEHVMAAFAPSLEECQKAQIFTQMQALKYIGNKVRRQRMWGGPKKTKMEEARELLASTILTHVLVKEFNFRAKCIYTAVMVRRVILAQGENKVDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSELKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAISTGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMEDGPIIKLASNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHQKLVNTFRIMRRAGYINEFVSISTNLSDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHEGLVEYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIELIDFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQTPLEGSSVPQQPQYKDVPVTYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLIVPQEDMPFCDTGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLIRHGYNYLGKDYVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGYSGWCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKLAKYNE

Target ORF information:

RefSeq Version	XM_011593838.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis polymerase (RNA) III (DNA directed) polypeptide B (POLR3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011593838.1

ORF Insert Sequence:

ATGGAGGCGC TGGCCACGGA GCTGGGGGCT CTGACTCCGG AGCAAGCGGC GGCTCCTGTG 
AACACGGTGG AGGAAAAATG GAGACTTCTT CCAGCATTTC TAAAGGTGAA AGGACTCGTG 
AAGCAGCACA TAGACTCATT CAACTATTTC ATCAATGTAG AGATAAAGAA AATCATGAAG 
GCCAATGAAA AAGTTACAAG TGATGCTGAC CCAATGTGGT ACTTGAAGTA CCTCAATATC 
TATGTGGGTA CTCCAGATGT GGAAGAAAGC TTCAATGTGA CACGACCTGT ATCGCCTCAT 
GAGTGCCGCT TGAGAGACAT GACTTATTCT GCCCCGATTA CAGTGGACAT TGAATATACA 
CGAGGTAGCC AGAGGATCAT ACGCAATGCA TTACCCATTG GCAGAATGCC TATAATGCTG 
CGTAGCTCCA ATTGTGTTCT TACTGGGAAA ACTCCAGCAG AATTTGCCAA ACTTAATGAA 
TGTCCTTTAG ATCCAGGTGG CTATTTCATT GTTAAAGGTG TAGAAAAAGT CATCCTTATT 
CAAGAGCAGT TATCCAAAAA CAGAATCATT GTGGAAGCTG ATAGAAAAGG CACAGTTGGC 
GCTTCTGTTA CCAGTTCCAC TCATGAGAAG AAGAGCAGAA CAAATATGGT TGTGAAACAA 
GGAAGATTTT ACCTCAGGCA CAACACTCTG TCAGAAGATA TTCCCATTGC TATCATATTT 
AAGGCCATGG GTGTTGAGAG TGACCAAGAG ATTGTACAAA TGATTGGCAC AGAGGAACAT 
GTGATGGCTG CATTTGCTCC TAGTCTGGAA GAATGCCAAA AAGCTCAGAT TTTTACACAG 
ATGCAGGCAT TGAAGTACAT TGGAAACAAA GTACGAAGGC AAAGGATGTG GGGAGGCCCA 
AAGAAAACAA AGATGGAAGA AGCACGAGAG CTGCTAGCAT CAACTATTCT GACCCACGTT 
CTTGTGAAGG AATTCAATTT TCGTGCCAAG TGCATATACA CAGCAGTGAT GGTGCGCAGA 
GTAATCTTGG CTCAAGGAGA AAATAAAGTA GATGACAGAG ACTACTATGG AAATAAACGT 
TTGGAGTTGG CAGGGCAGCT TCTGTCTCTT CTATTTGAAG ATTTGTTCAA AAAATTTAAC 
TCTGAACTGA AAAAGATTGC TGACCAGGTA ATCCCCAAGC AACGTGCAGC TCAGTTTGAT 
GTAGTGAAGC ACATGCGTCA AGACCAAATC ACCAATGGCA TGGTCAATGC TATCTCCACA 
GGAAATTGGT CTCTGAAGAG ATTCAAAATG GACCGCCAAG GCGTGACACA AGTATTGTCC 
CGCTTGTCTT ACATATCTGC TCTGGGAATG ATGACTAGAA TCTCTTCTCA GTTTGAAAAG 
ACAAGGAAAG TGAGTGGTCC TCGGTCACTA CAACCATCTC AGTGGGGGAT GCTCTGCCCA 
TCTGACACTC CTGAAGGAGA GGCTTGTGGT TTGGTTAAAA ACCTTGCCCT CATGACTCAC 
ATTACTACAG ATATGGAAGA TGGCCCAATT ATTAAATTAG CCAGTAATCT TGGAGTTGAA 
GATGTCAACT TGCTCTGTGG TGAAGAACTT TCATACCCAA ATGTGTTCCT TGTTTTCCTT 
AATGGTAACA TCCTCGGTGT CATACGTGAT CATCAGAAAT TAGTGAACAC GTTTCGGATA 
ATGCGTCGAG CGGGTTATAT CAATGAATTT GTTTCCATCT CTACAAATCT TTCTGACAGA 
TGTGTCTACA TTTCCTCTGA TGGAGGGAGA TTGTGCAGAC CCTACATAAT TGTCAAGAAG 
CAAAAACCTG CGGTTACAAA CAAGCATATG GAAGAACTGG CTCAGGGATA CAGGAATTTT 
GAAGACTTCT TGCACGAAGG CCTTGTTGAG TATTTGGATG TGAATGAAGA AAATGACTGT 
AACATTGCAC TATATGAACA CACAATTAAT AAAGATACAA CGCATTTGGA GATTGAGCCT 
TTCACGCTTC TTGGTGTCTG TGCTGGGCTG ATTCCGTATC CTCACCACAA CCAGTCCCCA 
AGAAACACTT ATCAGTGTGC CATGGGGAAA CAAGCAATGG GTACTATTGG ATACAATCAA 
AGAAACAGGA TAGATACTCT TATGTATCTT CTAGCCTATC CACAAAAGCC AATGGTAAAA 
ACAAAAACAA TTGAACTGAT AGATTTTGAG AAACTGCCTG CTGGACAGAA TGCCACAGTT 
GCTGTGATGA GCTACAGTGG TTACGATATT GAAGATGCTC TTGTCTTAAA CAAGGCCTCT 
TTGGATAGAG GTTTTGGAAG GTGTCTTGTA TACAAGAACG CCAAGTGTAC ACTGAAGCGT 
TACACAAACC AGACATTTGA TAAAGTTATG GGTCCGATGT TGGATGCAGC TACCCGTAAA 
CCAATCTGGC GCCATGAAAT TTTAGATGCT GATGGTATCT GCTCTCCAGG TGAAAAAGTA 
GAAAATAAGC AAGTCCTTGT GAACAAATCT ATGCCAACTG TGACCCAGAC ACCTCTGGAA 
GGAAGTAGTG TGCCTCAGCA GCCACAGTAC AAAGATGTGC CAGTAACCTA CAAAGGTGCG 
ACTGATTCTT ATATTGAAAA AGTAATGATT TCATCAAATG CAGAGGATGC TTTCTTGATA 
AAAATGTTGC TGAGGCAAAC TAGACGTCCA GAAATTGGTG ATAAATTTAG CAGTCGTCAT 
GGGCAGAAAG GGGTGTGTGG ACTGATTGTT CCTCAGGAAG ACATGCCATT TTGCGATACA 
GGAATCTGTC CAGATATCAT AATGAACCCT CATGGCTTCC CATCGCGTAT GACGGTAGGA 
AAGTTAATTG AACTCCTGGC TGGAAAGGCT GGGGTCCTTG ACGGCAGGTT TCACTATGGA 
ACAGCTTTTG GAGGCAGTAA AGTTAAGGAT GTGTGTGAAG ATCTTATTCG CCATGGTTAT 
AACTACCTAG GGAAGGACTA TGTAACATCT GGTATCACTG GGGAACCTTT GGAAGCATAT 
ATCTATTTTG GCCCTGTGTA TTACCAGAAA CTAAAGCACA TGGTGTTGGA TAAAATGCAT 
GCAAGAGCTC GAGGACCCAG AGCAGTGCTC ACCAGGCAAC CCACTGAAGG TCGATCCCGT 
GATGGTGGTT TACGTCTTGG AGAGATGGAA CGGGATTGTT TGATAGGTTA TGGAGCCAGC 
ATGCTTTTAC TGGAGAGACT GATGATTTCA AGTGATGCCT TTGAAGTGGA TGTTTGTGGG 
CAGTGTGGCT TGCTGGGGTA CTCTGGCTGG TGCCACTACT GCAAATCATC ATGTCATGTG 
TCTTCTCTGC GCATACCTTA TGCCTGTAAA CTCTTATTCC AAGAACTGCA GTCAATGAAC 
ATCATACCTA GGTTAAAACT AGCTAAGTAC AATGAATGA