PDE5A cDNA ORF clone, Aquila chrysaetos canadensis

Gene
Clones
gRNAs

Summary

Gene Symbol	PDE5A
Entrez Gene ID	105405615
Full Name	phosphodiesterase 5A
Gene Type	protein-coding
Organism	Aquila chrysaetos canadensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_011583391.1	XP_011581693.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
XM_011583392.1	XP_011581694.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2

Pathways from BioSystems
Gene Ontology

The following PDE5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAq20932	XM_011583391.1 Latest version!	Aquila chrysaetos canadensis phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OAq20933	XM_011583392.1 Latest version!	Aquila chrysaetos canadensis phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDE5A ( XM_011583391.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011581693.1 Aquila chrysaetos canadensis phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAq20932
Clone ID Related Accession (Same CDS sequence)	XM_011583391.1
Accession Version	XM_011583391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCTT TCGGAGACCA GACGAGAGAA ATGGTTAATG CATGGTTTGC TGAGAGAGTT 
CATACCATCC CAGTCTGCAA GGAGGGAACC AAGTCCCAGA GTGAATCCTG TGCTTGTCAC 
CAGCCTGCCT GTGCTGAGAC CACCAACTCT GGGACGCCAG CGAGGAAAAT CTCTGCTTCT 
GAATTTGACA GACCCTTAAG GCCTATTTTT GTCAAGGACT CAGTAGGAGC AGTGAGCTTC 
CTCTCCGGCG CTGAAAAGAA GGAACAGATG CCTCTGCAAT CTCCAAGGAT TGAGACCAGT 
GCAGGCGATC AGTGCTCAAG ACTATTAGAA CTTGTGAAAG ATATTTCTAG TCACTTAGAT 
GTCACAGCGC TTTGCCATAA AATTTTCCTA CATATCCATG AGCTTATAGC AGCTGATCGC 
TATTCCCTGT TCTTGGTCTG TGAGGATAGC TCTAATGAGA AGTTTCTTGT AAGCAGGCTG 
TTTGACGTGG CAGAAGGCTC CACCCTGGAA GAAGCTTCCA ACAACTGCAT TCGCCTGGAG 
TGGAACAAGG GCATCGTGGG TCATGTAGCA GCTATAGGCC AGCCTCTGAA CATCAAGAAC 
GCGTATGAGG ATCCAAGATT CAATGCAGAA GTTGACCAGA TCACAGGGTA TAAGACTCAG 
AGTATTCTCT GTATGCCAAT AAAGAATCAT AGAGAAGAGG TTGTTGGTGT GGCTCAAGCA 
ATCAATAAGA AATCTGGAAT TGGTGGCACT TTCACTGAAC AAGATGAAAA GGATTTTGCT 
GCGTATTTGG CATTTTGTGG AATTGTTCTT CACAACGCTC AATTGTATGA GACATCTTTG 
CTTGAGAACA GGCGTAATCA GGTGCTTCTT GATCTTGCCA GCCTGATTTT TGAAGAGCAG 
CAGTCTTTGG AAGTAATTCT GAAGAAGATA GCTGCCACTA TAATATCCTT CATGCAAGTG 
CAGAGGTGTA CAATCTTCAT AGTGGATGAA GACTGTACTG ATTCATTTTC TAGTGTGTTT 
CACATGGAAT CTGAGGAATT AGAAGATTCA GCTGAAAATC TGAAGAGGGA CTATGATACA 
AACAAAATCA ATTATATGTA TGCTGAGTAT GTCAAGAATA CGATGGAGCC TCTTAACATC 
CCAGATGTCT GCAAAGACAG AAGATTTCCC TGGACAAATG ACAATGCAGA AAATGTAAGT 
CAACACATAA AGAGTTTGCT GTGTACACCA ATAAAAAATG GGAAAAAGAA TAAAGTGATA 
GGGGTTTGCC AGCTTGTGAA TAAGATGGAA GAAAACTCGG GCAAAATCAA GGCTTTCAAC 
AGAAATGATG AACAGTTCCT GGAAGCATTT GTCATCTTTT GTGGCTTGGG GATCCAGAAT 
ACACAGATGT ACGAAGCTGT AGAAAGAGCC ATGGCCAAAC AGATGGTGAC ATTAGAGGTT 
CTCTCATACC ATGCTTCTGC TGCTGAGGAG GAAACGAGGG AGCTGCAGGT AACAGCGGCT 
GCTGTAGTAC CATCTGCACA ATCTCTCAAC CTAACTGATT TCAACTTCAG TGATTTTGAG 
CTATCAGATT TTGAAACAAC ACTGTGTACA ATTCGAATGT TTACAGACCT CAACCTGGTG 
CAAAACTTCC AAATGAAACA TGAGGTTCTC TGCAGATGGA TTTTAAGTGT AAAGAAAAAT 
TATCGGAAAA ATGTTGCTTA TCACAATTGG AGACATGCCT TTAATACAGC GCAGTGCATG 
TTTGCTGCTT TAAAATCTGG TAAAATTCAG AGCAAACTGA CTGACTTAGA AACACTGGCT 
CTGCTGATAG CAACTCTAAG CCATGATTTG GATCACAGGG GAGTGAACAA CTCTTACATA 
CAAAGAAGCG AACATCCACT GGCTCAACTG TATTGCCACT CAATCATGGA GCATCATCAT 
TTTGATCAAT GCCTTATGAT CCTGAATAGT CCAGGAAATC AGATTCTCAG CAGCCTTTCC 
ATTGAAGAAT ACAAGGCCAC ACTGAAAATG ATAAAACAAG CCATTCTTGC CACAGACCTA 
GCACTATACA TAAAGAGGAG AGGAGAATTT TTTGAACTTC TAAGGAAGAA GCAATTTAAT 
TGGGAAGATC CTACACAGAA GGAGCTATTT TTAGCAATGC TGATGACTGC TTGTGATTTA 
TCAGCCATAA CTAAACCATG GCCAGTTCAG CAACGGATTG CTGAGCTTGT AGCTACTGAG 
TTCTTTGATC AAGGAGACAA AGAGCGGAAG GAACTCAACA TAGAACCAAC AGATCTAATG 
AACAGAGAGA AGAAGAATAA AATTCCTAGC ATGCAGGTTG GATTCATAGA TGCTGTTTGT 
TTGCAGCTGT ATGAGGCCCT AACGCACGTG TCAGAGGCCT GCTACCCTTT ACTGGATGGC 
TGTAGAAAAA ATAGGCAGAA ATGGCAATCC TTAGCTGAAC AACAAGAGAA GAATCTGATT 
AATGGAGAAA GCAATCAAGC CAAACGGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011581693.1
CDS	35..2527
Translation	MLPFGDQTREMVNAWFAERVHTIPVCKEGTKSQSESCACHQPACAETTNSGTPARKISASEFDRPLRPIFVKDSVGAVSFLSGAEKKEQMPLQSPRIETSAGDQCSRLLELVKDISSHLDVTALCHKIFLHIHELIAADRYSLFLVCEDSSNEKFLVSRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAAIGQPLNIKNAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGIGGTFTEQDEKDFAAYLAFCGIVLHNAQLYETSLLENRRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQRCTIFIVDEDCTDSFSSVFHMESEELEDSAENLKRDYDTNKINYMYAEYVKNTMEPLNIPDVCKDRRFPWTNDNAENVSQHIKSLLCTPIKNGKKNKVIGVCQLVNKMEENSGKIKAFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQVTAAAVVPSAQSLNLTDFNFSDFELSDFETTLCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKSGKIQSKLTDLETLALLIATLSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSSLSIEEYKATLKMIKQAILATDLALYIKRRGEFFELLRKKQFNWEDPTQKELFLAMLMTACDLSAITKPWPVQQRIAELVATEFFDQGDKERKELNIEPTDLMNREKKNKIPSMQVGFIDAVCLQLYEALTHVSEACYPLLDGCRKNRQKWQSLAEQQEKNLINGESNQAKRN

Target ORF information:

RefSeq Version	XM_011583391.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011583391.1

ORF Insert Sequence:

ATGCTGCCTT TCGGAGACCA GACGAGAGAA ATGGTTAATG CATGGTTTGC TGAGAGAGTT 
CATACCATCC CAGTCTGCAA GGAGGGAACC AAGTCCCAGA GTGAATCCTG TGCTTGTCAC 
CAGCCTGCCT GTGCTGAGAC CACCAACTCT GGGACGCCAG CGAGGAAAAT CTCTGCTTCT 
GAATTTGACA GACCCTTAAG GCCTATTTTT GTCAAGGACT CAGTAGGAGC AGTGAGCTTC 
CTCTCCGGCG CTGAAAAGAA GGAACAGATG CCTCTGCAAT CTCCAAGGAT TGAGACCAGT 
GCAGGCGATC AGTGCTCAAG ACTATTAGAA CTTGTGAAAG ATATTTCTAG TCACTTAGAT 
GTCACAGCGC TTTGCCATAA AATTTTCCTA CATATCCATG AGCTTATAGC AGCTGATCGC 
TATTCCCTGT TCTTGGTCTG TGAGGATAGC TCTAATGAGA AGTTTCTTGT AAGCAGGCTG 
TTTGACGTGG CAGAAGGCTC CACCCTGGAA GAAGCTTCCA ACAACTGCAT TCGCCTGGAG 
TGGAACAAGG GCATCGTGGG TCATGTAGCA GCTATAGGCC AGCCTCTGAA CATCAAGAAC 
GCGTATGAGG ATCCAAGATT CAATGCAGAA GTTGACCAGA TCACAGGGTA TAAGACTCAG 
AGTATTCTCT GTATGCCAAT AAAGAATCAT AGAGAAGAGG TTGTTGGTGT GGCTCAAGCA 
ATCAATAAGA AATCTGGAAT TGGTGGCACT TTCACTGAAC AAGATGAAAA GGATTTTGCT 
GCGTATTTGG CATTTTGTGG AATTGTTCTT CACAACGCTC AATTGTATGA GACATCTTTG 
CTTGAGAACA GGCGTAATCA GGTGCTTCTT GATCTTGCCA GCCTGATTTT TGAAGAGCAG 
CAGTCTTTGG AAGTAATTCT GAAGAAGATA GCTGCCACTA TAATATCCTT CATGCAAGTG 
CAGAGGTGTA CAATCTTCAT AGTGGATGAA GACTGTACTG ATTCATTTTC TAGTGTGTTT 
CACATGGAAT CTGAGGAATT AGAAGATTCA GCTGAAAATC TGAAGAGGGA CTATGATACA 
AACAAAATCA ATTATATGTA TGCTGAGTAT GTCAAGAATA CGATGGAGCC TCTTAACATC 
CCAGATGTCT GCAAAGACAG AAGATTTCCC TGGACAAATG ACAATGCAGA AAATGTAAGT 
CAACACATAA AGAGTTTGCT GTGTACACCA ATAAAAAATG GGAAAAAGAA TAAAGTGATA 
GGGGTTTGCC AGCTTGTGAA TAAGATGGAA GAAAACTCGG GCAAAATCAA GGCTTTCAAC 
AGAAATGATG AACAGTTCCT GGAAGCATTT GTCATCTTTT GTGGCTTGGG GATCCAGAAT 
ACACAGATGT ACGAAGCTGT AGAAAGAGCC ATGGCCAAAC AGATGGTGAC ATTAGAGGTT 
CTCTCATACC ATGCTTCTGC TGCTGAGGAG GAAACGAGGG AGCTGCAGGT AACAGCGGCT 
GCTGTAGTAC CATCTGCACA ATCTCTCAAC CTAACTGATT TCAACTTCAG TGATTTTGAG 
CTATCAGATT TTGAAACAAC ACTGTGTACA ATTCGAATGT TTACAGACCT CAACCTGGTG 
CAAAACTTCC AAATGAAACA TGAGGTTCTC TGCAGATGGA TTTTAAGTGT AAAGAAAAAT 
TATCGGAAAA ATGTTGCTTA TCACAATTGG AGACATGCCT TTAATACAGC GCAGTGCATG 
TTTGCTGCTT TAAAATCTGG TAAAATTCAG AGCAAACTGA CTGACTTAGA AACACTGGCT 
CTGCTGATAG CAACTCTAAG CCATGATTTG GATCACAGGG GAGTGAACAA CTCTTACATA 
CAAAGAAGCG AACATCCACT GGCTCAACTG TATTGCCACT CAATCATGGA GCATCATCAT 
TTTGATCAAT GCCTTATGAT CCTGAATAGT CCAGGAAATC AGATTCTCAG CAGCCTTTCC 
ATTGAAGAAT ACAAGGCCAC ACTGAAAATG ATAAAACAAG CCATTCTTGC CACAGACCTA 
GCACTATACA TAAAGAGGAG AGGAGAATTT TTTGAACTTC TAAGGAAGAA GCAATTTAAT 
TGGGAAGATC CTACACAGAA GGAGCTATTT TTAGCAATGC TGATGACTGC TTGTGATTTA 
TCAGCCATAA CTAAACCATG GCCAGTTCAG CAACGGATTG CTGAGCTTGT AGCTACTGAG 
TTCTTTGATC AAGGAGACAA AGAGCGGAAG GAACTCAACA TAGAACCAAC AGATCTAATG 
AACAGAGAGA AGAAGAATAA AATTCCTAGC ATGCAGGTTG GATTCATAGA TGCTGTTTGT 
TTGCAGCTGT ATGAGGCCCT AACGCACGTG TCAGAGGCCT GCTACCCTTT ACTGGATGGC 
TGTAGAAAAA ATAGGCAGAA ATGGCAATCC TTAGCTGAAC AACAAGAGAA GAATCTGATT 
AATGGAGAAA GCAATCAAGC CAAACGGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE5A ( XM_011583392.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011581694.1 Aquila chrysaetos canadensis phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAq20933
Clone ID Related Accession (Same CDS sequence)	XM_011583392.1
Accession Version	XM_011583392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCTT TCGGAGACCA GACGAGAGAA ATGGTTAATG CATGGTTTGC TGAGAGAGTT 
CATACCATCC CAGTCTGCAA GGAGGGAACC AAGTCCCAGA GTGAATCCTG TGCTTGTCAC 
CAGCCTGCCT GTGCTGAGAC CACCAACTCT GGGACGCCAG CGAGGAAAAT CTCTGCTTCT 
GAATTTGACA GACCCTTAAG GCCTATTTTT GTCAAGGACT CAGTAGGAGC AGTGAGCTTC 
CTCTCCGGCG CTGAAAAGAA GGAACAGATG CCTCTGCAAT CTCCAAGGAT TGAGACCAGT 
GCAGGCGATC AGTGCTCAAG ACTATTAGAA CTTGTGAAAG ATATTTCTAG TCACTTAGAT 
GTCACAGCGC TTTGCCATAA AATTTTCCTA CATATCCATG AGCTTATAGC AGCTGATCGC 
TATTCCCTGT TCTTGGTCTG TGAGGATAGC TCTAATGAGA AGTTTCTTGT AAGCAGGCTG 
TTTGACGTGG CAGAAGGCTC CACCCTGGAA GAAGCTTCCA ACAACTGCAT TCGCCTGGAG 
TGGAACAAGG GCATCGTGGG TCATGTAGCA GCTATAGGCC AGCCTCTGAA CATCAAGAAC 
GCGTATGAGG ATCCAAGATT CAATGCAGAA GTTGACCAGA TCACAGGGTA TAAGACTCAG 
AGTATTCTCT GTATGCCAAT AAAGAATCAT AGAGAAGAGG TTGTTGGTGT GGCTCAAGCA 
ATCAATAAGA AATCTGGAAT TGGTGGCACT TTCACTGAAC AAGATGAAAA GGATTTTGCT 
GCGTATTTGG CATTTTGTGG AATTGTTCTT CACAACGCTC AATTGTATGA GACATCTTTG 
CTTGAGAACA GGCGTAATCA GGTGCTTCTT GATCTTGCCA GCCTGATTTT TGAAGAGCAG 
CAGTCTTTGG AAGTAATTCT GAAGAAGATA GCTGCCACTA TAATATCCTT CATGCAAGTG 
CAGAGGTGTA CAATCTTCAT AGTGGATGAA GACTGTACTG ATTCATTTTC TAGTGTGTTT 
CACATGGAAT CTGAGGAATT AGAAGATTCA GCTGAAAATC TGAAGAGGGA CTATGATACA 
AACAAAATCA ATTATATGTA TGCTGAGTAT GTCAAGAATA CGATGGAGCC TCTTAACATC 
CCAGATGTCT GCAAAGACAG AAGATTTCCC TGGACAAATG ACAATGCAGA AAATGTAAGT 
CAACACATAA AGAGTTTGCT GTGTACACCA ATAAAAAATG GGAAAAAGAA TAAAGTGATA 
GGGGTTTGCC AGCTTGTGAA TAAGATGGAA GAAAACTCGG GCAAAATCAA GGCTTTCAAC 
AGAAATGATG AACAGTTCCT GGAAGCATTT GTCATCTTTT GTGGCTTGGG GATCCAGAAT 
ACACAGATGT ACGAAGCTGT AGAAAGAGCC ATGGCCAAAC AGATGGTGAC ATTAGAGGTT 
CTCTCATACC ATGCTTCTGC TGCTGAGGAG GAAACGAGGG AGCTGCAGGC TGCTGTAGTA 
CCATCTGCAC AATCTCTCAA CCTAACTGAT TTCAACTTCA GTGATTTTGA GCTATCAGAT 
TTTGAAACAA CACTGTGTAC AATTCGAATG TTTACAGACC TCAACCTGGT GCAAAACTTC 
CAAATGAAAC ATGAGGTTCT CTGCAGATGG ATTTTAAGTG TAAAGAAAAA TTATCGGAAA 
AATGTTGCTT ATCACAATTG GAGACATGCC TTTAATACAG CGCAGTGCAT GTTTGCTGCT 
TTAAAATCTG GTAAAATTCA GAGCAAACTG ACTGACTTAG AAACACTGGC TCTGCTGATA 
GCAACTCTAA GCCATGATTT GGATCACAGG GGAGTGAACA ACTCTTACAT ACAAAGAAGC 
GAACATCCAC TGGCTCAACT GTATTGCCAC TCAATCATGG AGCATCATCA TTTTGATCAA 
TGCCTTATGA TCCTGAATAG TCCAGGAAAT CAGATTCTCA GCAGCCTTTC CATTGAAGAA 
TACAAGGCCA CACTGAAAAT GATAAAACAA GCCATTCTTG CCACAGACCT AGCACTATAC 
ATAAAGAGGA GAGGAGAATT TTTTGAACTT CTAAGGAAGA AGCAATTTAA TTGGGAAGAT 
CCTACACAGA AGGAGCTATT TTTAGCAATG CTGATGACTG CTTGTGATTT ATCAGCCATA 
ACTAAACCAT GGCCAGTTCA GCAACGGATT GCTGAGCTTG TAGCTACTGA GTTCTTTGAT 
CAAGGAGACA AAGAGCGGAA GGAACTCAAC ATAGAACCAA CAGATCTAAT GAACAGAGAG 
AAGAAGAATA AAATTCCTAG CATGCAGGTT GGATTCATAG ATGCTGTTTG TTTGCAGCTG 
TATGAGGCCC TAACGCACGT GTCAGAGGCC TGCTACCCTT TACTGGATGG CTGTAGAAAA 
AATAGGCAGA AATGGCAATC CTTAGCTGAA CAACAAGAGA AGAATCTGAT TAATGGAGAA 
AGCAATCAAG CCAAACGGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011581694.1
CDS	35..2518
Translation	MLPFGDQTREMVNAWFAERVHTIPVCKEGTKSQSESCACHQPACAETTNSGTPARKISASEFDRPLRPIFVKDSVGAVSFLSGAEKKEQMPLQSPRIETSAGDQCSRLLELVKDISSHLDVTALCHKIFLHIHELIAADRYSLFLVCEDSSNEKFLVSRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAAIGQPLNIKNAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGIGGTFTEQDEKDFAAYLAFCGIVLHNAQLYETSLLENRRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQRCTIFIVDEDCTDSFSSVFHMESEELEDSAENLKRDYDTNKINYMYAEYVKNTMEPLNIPDVCKDRRFPWTNDNAENVSQHIKSLLCTPIKNGKKNKVIGVCQLVNKMEENSGKIKAFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQAAVVPSAQSLNLTDFNFSDFELSDFETTLCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKSGKIQSKLTDLETLALLIATLSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSSLSIEEYKATLKMIKQAILATDLALYIKRRGEFFELLRKKQFNWEDPTQKELFLAMLMTACDLSAITKPWPVQQRIAELVATEFFDQGDKERKELNIEPTDLMNREKKNKIPSMQVGFIDAVCLQLYEALTHVSEACYPLLDGCRKNRQKWQSLAEQQEKNLINGESNQAKRN

Target ORF information:

RefSeq Version	XM_011583392.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011583392.1

ORF Insert Sequence:

ATGCTGCCTT TCGGAGACCA GACGAGAGAA ATGGTTAATG CATGGTTTGC TGAGAGAGTT 
CATACCATCC CAGTCTGCAA GGAGGGAACC AAGTCCCAGA GTGAATCCTG TGCTTGTCAC 
CAGCCTGCCT GTGCTGAGAC CACCAACTCT GGGACGCCAG CGAGGAAAAT CTCTGCTTCT 
GAATTTGACA GACCCTTAAG GCCTATTTTT GTCAAGGACT CAGTAGGAGC AGTGAGCTTC 
CTCTCCGGCG CTGAAAAGAA GGAACAGATG CCTCTGCAAT CTCCAAGGAT TGAGACCAGT 
GCAGGCGATC AGTGCTCAAG ACTATTAGAA CTTGTGAAAG ATATTTCTAG TCACTTAGAT 
GTCACAGCGC TTTGCCATAA AATTTTCCTA CATATCCATG AGCTTATAGC AGCTGATCGC 
TATTCCCTGT TCTTGGTCTG TGAGGATAGC TCTAATGAGA AGTTTCTTGT AAGCAGGCTG 
TTTGACGTGG CAGAAGGCTC CACCCTGGAA GAAGCTTCCA ACAACTGCAT TCGCCTGGAG 
TGGAACAAGG GCATCGTGGG TCATGTAGCA GCTATAGGCC AGCCTCTGAA CATCAAGAAC 
GCGTATGAGG ATCCAAGATT CAATGCAGAA GTTGACCAGA TCACAGGGTA TAAGACTCAG 
AGTATTCTCT GTATGCCAAT AAAGAATCAT AGAGAAGAGG TTGTTGGTGT GGCTCAAGCA 
ATCAATAAGA AATCTGGAAT TGGTGGCACT TTCACTGAAC AAGATGAAAA GGATTTTGCT 
GCGTATTTGG CATTTTGTGG AATTGTTCTT CACAACGCTC AATTGTATGA GACATCTTTG 
CTTGAGAACA GGCGTAATCA GGTGCTTCTT GATCTTGCCA GCCTGATTTT TGAAGAGCAG 
CAGTCTTTGG AAGTAATTCT GAAGAAGATA GCTGCCACTA TAATATCCTT CATGCAAGTG 
CAGAGGTGTA CAATCTTCAT AGTGGATGAA GACTGTACTG ATTCATTTTC TAGTGTGTTT 
CACATGGAAT CTGAGGAATT AGAAGATTCA GCTGAAAATC TGAAGAGGGA CTATGATACA 
AACAAAATCA ATTATATGTA TGCTGAGTAT GTCAAGAATA CGATGGAGCC TCTTAACATC 
CCAGATGTCT GCAAAGACAG AAGATTTCCC TGGACAAATG ACAATGCAGA AAATGTAAGT 
CAACACATAA AGAGTTTGCT GTGTACACCA ATAAAAAATG GGAAAAAGAA TAAAGTGATA 
GGGGTTTGCC AGCTTGTGAA TAAGATGGAA GAAAACTCGG GCAAAATCAA GGCTTTCAAC 
AGAAATGATG AACAGTTCCT GGAAGCATTT GTCATCTTTT GTGGCTTGGG GATCCAGAAT 
ACACAGATGT ACGAAGCTGT AGAAAGAGCC ATGGCCAAAC AGATGGTGAC ATTAGAGGTT 
CTCTCATACC ATGCTTCTGC TGCTGAGGAG GAAACGAGGG AGCTGCAGGC TGCTGTAGTA 
CCATCTGCAC AATCTCTCAA CCTAACTGAT TTCAACTTCA GTGATTTTGA GCTATCAGAT 
TTTGAAACAA CACTGTGTAC AATTCGAATG TTTACAGACC TCAACCTGGT GCAAAACTTC 
CAAATGAAAC ATGAGGTTCT CTGCAGATGG ATTTTAAGTG TAAAGAAAAA TTATCGGAAA 
AATGTTGCTT ATCACAATTG GAGACATGCC TTTAATACAG CGCAGTGCAT GTTTGCTGCT 
TTAAAATCTG GTAAAATTCA GAGCAAACTG ACTGACTTAG AAACACTGGC TCTGCTGATA 
GCAACTCTAA GCCATGATTT GGATCACAGG GGAGTGAACA ACTCTTACAT ACAAAGAAGC 
GAACATCCAC TGGCTCAACT GTATTGCCAC TCAATCATGG AGCATCATCA TTTTGATCAA 
TGCCTTATGA TCCTGAATAG TCCAGGAAAT CAGATTCTCA GCAGCCTTTC CATTGAAGAA 
TACAAGGCCA CACTGAAAAT GATAAAACAA GCCATTCTTG CCACAGACCT AGCACTATAC 
ATAAAGAGGA GAGGAGAATT TTTTGAACTT CTAAGGAAGA AGCAATTTAA TTGGGAAGAT 
CCTACACAGA AGGAGCTATT TTTAGCAATG CTGATGACTG CTTGTGATTT ATCAGCCATA 
ACTAAACCAT GGCCAGTTCA GCAACGGATT GCTGAGCTTG TAGCTACTGA GTTCTTTGAT 
CAAGGAGACA AAGAGCGGAA GGAACTCAAC ATAGAACCAA CAGATCTAAT GAACAGAGAG 
AAGAAGAATA AAATTCCTAG CATGCAGGTT GGATTCATAG ATGCTGTTTG TTTGCAGCTG 
TATGAGGCCC TAACGCACGT GTCAGAGGCC TGCTACCCTT TACTGGATGG CTGTAGAAAA 
AATAGGCAGA AATGGCAATC CTTAGCTGAA CAACAAGAGA AGAATCTGAT TAATGGAGAA 
AGCAATCAAG CCAAACGGAA CTGA