USP53 cDNA ORF clone, Aquila chrysaetos canadensis

Gene
Clones
gRNAs

Summary

Gene Symbol	USP53
Entrez Gene ID	105405613
Full Name	ubiquitin specific peptidase 53
Gene Type	protein-coding
Organism	Aquila chrysaetos canadensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_011583388.1	XP_011581690.1	inactive ubiquitin carboxyl-terminal hydrolase 53

Pathways from BioSystems
Gene Ontology

The following USP53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAq20930	XM_011583388.1 Latest version!	Aquila chrysaetos canadensis ubiquitin specific peptidase 53 (USP53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP53 ( XM_011583388.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011581690.1 Aquila chrysaetos canadensis ubiquitin specific peptidase 53 (USP53), mRNA.

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CloneID	OAq20930
Clone ID Related Accession (Same CDS sequence)	XM_011583388.1
Accession Version	XM_011583388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	inactive ubiquitin carboxyl-terminal hydrolase 53
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950912.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGGG TGAAATTCTT AAGAAAACCT GGGGGTAACC TTGGAAAAGT TTATCAGCCT 
GGAAGTATAC TGTCCCTGGC TCCTACAAAA GGCTTGTTAA ATGAACCGGG ACAAAACAGC 
TGCTTTCTTA ATAGTGCTGT TCAGGTATTA TGGCAACTTG ACATATTTCG ACGAAGTTTA 
AGAGGTTTAA CTGGACATGT GTGTCAGGGA GATGCGTGCA TATTTTGTGC TTTAAAGGCA 
ATATTTTCAC AATTTCAACA CAGTCGAGAA AAAGCACTCC CATCAGATAA TATGAGACAT 
GCCCTGGCAG AAAGTTTTAA GGATGAACAG CGTTTCCAGC TTGGATTCAT GGATGATGCA 
GCAGAATGCT TTGAAAATAT CCTTGAGAGG ATTCATTTTC ACCTAGTGCC AAACAGTGAG 
ACAGATATGT GCACTTCAAA ATCCTGTATT TCTCATCAGA AGTTTGCTAT GACACTGTAT 
GAACAGTGTG TATGTCGCAG TTGTGGAGCA TCATCAGACC CGTTGCCTTT TACGGAATTT 
GTGCGTTACA TTTCTACCAC AGCCCTGTGT AACGAAGTAG AAAGAATGAT GGAGAGGCAT 
GAACGTCTCA AGCCGGAAAT GTTTGCAGAA TTGCTGCAGG CAGCAAATAC TACTGATGAC 
TACAGAAAGT GTCCTAGTAA CTGTGGTCAA AAAATAAAAA TTCGTCGTGT TCTTATGAAT 
TGTCCTGAGA TTGTCACAAT CGGTTTAGTC TGGGATTCAG AACACTCTGA CCTGACAGAA 
GAGGTTATGC GGAATCTGGC AACACAACTT TATCTTCCTG GGCTGTTTTA TAGAGTCACA 
GATGAAAATG CCAAAAATAG TGAACTTTTT CTTGTGGGAA TGATTTGCTA CACAAGCCGA 
CATTACTGTG CCTTTGCCTT TCATACCAAG AGTTGCAAAT GGGTGCTGTT TGATGATGCT 
AATGTAAAAG AGATCGGAAC AAAATGGAAA GATGTGGTCT CCAGGTGCAT TCGATGTCAC 
TTTCAGCCAT TGTTGCTATT TTATGCTAAC CCAGATGGCA CAGCTGTATC TCCAGAAGAT 
GCTCCAAAGC AGATTATTCA CTGGTCTCAA TGCAAAGCAG CAGGAGGAAA TGGGGAAGAC 
TTGGGATTTG AAAAGCATTC TGCTCCTAAA TCAGACCACA TGAAAGAGAA TGGAGTTGGA 
GACCCCGCTA ATCAAAAGAG TAATAAAAAA CTTCAGCCAG ATAATTCAGT ATTCAGTAGA 
AGCCATCTTC AAGCTAGTAG TGGCAGAGGT CCAGCTAAGT TAGGTTACAG CGATCAAAAG 
GACAGGCTAA AGGACATATC CAGAGAGTGT GCTCAGAAAG CTGCTGATAT GAAAAACTTC 
TCATCTTTAC GAAAAGATGC AGACAGAGGA CCAAGAAAAG AGTCTGGGAG ACAAAGAGAC 
TTGATTGGGG AAGATCGTTC CAATGTTAAG TCGGGGTCTC CTCCAGTTGG AAATGGATTT 
AGACACTGTG CTGATCAGCG CATATACAGC AGCCAGGGAA GAGGCTCCTA TAAGCATGAC 
GGTGCACCTC ACCAAGCAAA GCTTTCTGTA CAGGTGCTTG GATCAAATAA AACAGAAGCG 
TTTCCTGCTG GGGAGAAACC AATGATCAGG ACCCGGACTG ATGGTGTCGC TGGCTATGAT 
ACAGACACCA GTCAAGACTC TAGGGACAAA GGAAATAGCA GCAACAGCAG AAGCAGAAGT 
AAAGGTTGGA AACCTATGCG AGAGACACTA AACGTAGACA GCATTTTCAG TGAGACTGAG 
AAAAAACAGC ATAGCCCAAA GCACAAGGTA AATCCGAACA GTAAATCTAA GCATGAAAAG 
GAGCGAAGCT TTAACCATTG GCCAAAAGAG AACCAAATCC AGAAAGGTTT AATGACTATA 
TATGAAGATG AAACAAAACA GGATACAGGA AGTAGAAGTT CACTGGATTC AGAGGGAAAA 
GGGAATGCTG AAAAAAGCAA AGGATTTACA GAGAGAAAAA TCCACAGTGA TAACTGGCAA 
ATTCAGAGGA CAGAATCTGG ATATGAAAGC AGTGATCATA TCAGCAATGG ATCTGCAAAT 
CCGGACTCGC CCATTATTGA AGGAATCAAT CCGGCAGATG TCAAGAATGT TAAAGAAAAT 
GCTTCCTGCA GTGAACAGAA CTTGTCAACC AAAAAAGCTG ATAATGTGTT ACATTCAGTA 
TCCCAGCAGA ACAGAAACTT TTATGACTTG AGGAAAGACC CAATCAATTC AGAAGTTAAC 
TACAGACCTC ATGTTAATTT CCCAACAGAA CTACATTTGC AGGTGCCCAG TCCTCCAGTA 
AAGAGAGCTG AAATGCCTGA GACCAATAGG AAACTTTTCC AATCATCAGC TCTACAGCCA 
GTTCTTAAAG ACATTGTTAA GTCAGAAAGT AGGAACAAGG CAAACGAACA GAATTCTTCA 
GAGTGGCTTA ATCCAGACAA GATTGAGAAG ATGAATGTGT CAGTATATTG TGCTGATGGC 
ATATCCCACC CCTATACAGA AAATGTCTGC GGAAGGAAGC TGATCAACAA TGACTTTCAC 
TCCTCTTCCC AGCCGCAGTC TCATTATGCA AGAACGTTTG GTCCCAAGGT GGGGTTGTCA 
CCTTGTGTGC CACAGAACCT GTCAGAAAGG CCCATGGTGC TTCCCCCTCC CGGTGAGCAT 
GCCGGGCACC CACTCCGAAG GGTGGATGCT CTTTGGGTGC CTGAAGCAGT GTACCAGAAT 
CTTCCTCCCC CTTTACCGCC CAAGAAATAC GCTCTGAATG CTTCGCCAGG ATCAGAAAAT 
AACTCAGCAG CTGATGTAAA ATCGACAGAA ATGTTGCAAA ATAATCCGGT AAGGCAAACA 
GGTACACCTT TAAAATCTGC ATCAGAAGCA AGCATATTTA CAAATGAACA AAGGCTTAAA 
GAGCCATTTC AAGGGAATGA ACTGTTTGTT CATAAACCGG ATTCTCCACC TCGCTTATCA 
GTTAATGACT TTTGGACTGT GTCTGAAAAC TTTTTAAAGA AAGGATCTCG TCATACGGGA 
CCAGGTGCTG GCTATGTGGA TGGAAATGAT AGCGTATCCC TAACGACATA TTTTTCAGTA 
GACAGTTGTA TGACTGACAC ATACAGGATG AAATATCATC AGAGACCCAA GCTGTATTTT 
ACAGATAGTG GAAGTTTTCA CAAAGAAAAA CATCCTCCAT CAGCTGGAGT AGAACTGAGT 
ACTACGTACC ACGCTACTCT TGAGCCCAGG CATCCCACAT CTGAGCAAAG GTACAAGGCA 
AATGTAGAAG GCATACACTG CAGTAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011581690.1
CDS	498..3827
Translation	MAWVKFLRKPGGNLGKVYQPGSILSLAPTKGLLNEPGQNSCFLNSAVQVLWQLDIFRRSLRGLTGHVCQGDACIFCALKAIFSQFQHSREKALPSDNMRHALAESFKDEQRFQLGFMDDAAECFENILERIHFHLVPNSETDMCTSKSCISHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMMERHERLKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEEVMRNLATQLYLPGLFYRVTDENAKNSELFLVGMICYTSRHYCAFAFHTKSCKWVLFDDANVKEIGTKWKDVVSRCIRCHFQPLLLFYANPDGTAVSPEDAPKQIIHWSQCKAAGGNGEDLGFEKHSAPKSDHMKENGVGDPANQKSNKKLQPDNSVFSRSHLQASSGRGPAKLGYSDQKDRLKDISRECAQKAADMKNFSSLRKDADRGPRKESGRQRDLIGEDRSNVKSGSPPVGNGFRHCADQRIYSSQGRGSYKHDGAPHQAKLSVQVLGSNKTEAFPAGEKPMIRTRTDGVAGYDTDTSQDSRDKGNSSNSRSRSKGWKPMRETLNVDSIFSETEKKQHSPKHKVNPNSKSKHEKERSFNHWPKENQIQKGLMTIYEDETKQDTGSRSSLDSEGKGNAEKSKGFTERKIHSDNWQIQRTESGYESSDHISNGSANPDSPIIEGINPADVKNVKENASCSEQNLSTKKADNVLHSVSQQNRNFYDLRKDPINSEVNYRPHVNFPTELHLQVPSPPVKRAEMPETNRKLFQSSALQPVLKDIVKSESRNKANEQNSSEWLNPDKIEKMNVSVYCADGISHPYTENVCGRKLINNDFHSSSQPQSHYARTFGPKVGLSPCVPQNLSERPMVLPPPGEHAGHPLRRVDALWVPEAVYQNLPPPLPPKKYALNASPGSENNSAADVKSTEMLQNNPVRQTGTPLKSASEASIFTNEQRLKEPFQGNELFVHKPDSPPRLSVNDFWTVSENFLKKGSRHTGPGAGYVDGNDSVSLTTYFSVDSCMTDTYRMKYHQRPKLYFTDSGSFHKEKHPPSAGVELSTTYHATLEPRHPTSEQRYKANVEGIHCSR

Target ORF information:

RefSeq Version	XM_011583388.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis ubiquitin specific peptidase 53 (USP53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011583388.1

ORF Insert Sequence:

ATGGCATGGG TGAAATTCTT AAGAAAACCT GGGGGTAACC TTGGAAAAGT TTATCAGCCT 
GGAAGTATAC TGTCCCTGGC TCCTACAAAA GGCTTGTTAA ATGAACCGGG ACAAAACAGC 
TGCTTTCTTA ATAGTGCTGT TCAGGTATTA TGGCAACTTG ACATATTTCG ACGAAGTTTA 
AGAGGTTTAA CTGGACATGT GTGTCAGGGA GATGCGTGCA TATTTTGTGC TTTAAAGGCA 
ATATTTTCAC AATTTCAACA CAGTCGAGAA AAAGCACTCC CATCAGATAA TATGAGACAT 
GCCCTGGCAG AAAGTTTTAA GGATGAACAG CGTTTCCAGC TTGGATTCAT GGATGATGCA 
GCAGAATGCT TTGAAAATAT CCTTGAGAGG ATTCATTTTC ACCTAGTGCC AAACAGTGAG 
ACAGATATGT GCACTTCAAA ATCCTGTATT TCTCATCAGA AGTTTGCTAT GACACTGTAT 
GAACAGTGTG TATGTCGCAG TTGTGGAGCA TCATCAGACC CGTTGCCTTT TACGGAATTT 
GTGCGTTACA TTTCTACCAC AGCCCTGTGT AACGAAGTAG AAAGAATGAT GGAGAGGCAT 
GAACGTCTCA AGCCGGAAAT GTTTGCAGAA TTGCTGCAGG CAGCAAATAC TACTGATGAC 
TACAGAAAGT GTCCTAGTAA CTGTGGTCAA AAAATAAAAA TTCGTCGTGT TCTTATGAAT 
TGTCCTGAGA TTGTCACAAT CGGTTTAGTC TGGGATTCAG AACACTCTGA CCTGACAGAA 
GAGGTTATGC GGAATCTGGC AACACAACTT TATCTTCCTG GGCTGTTTTA TAGAGTCACA 
GATGAAAATG CCAAAAATAG TGAACTTTTT CTTGTGGGAA TGATTTGCTA CACAAGCCGA 
CATTACTGTG CCTTTGCCTT TCATACCAAG AGTTGCAAAT GGGTGCTGTT TGATGATGCT 
AATGTAAAAG AGATCGGAAC AAAATGGAAA GATGTGGTCT CCAGGTGCAT TCGATGTCAC 
TTTCAGCCAT TGTTGCTATT TTATGCTAAC CCAGATGGCA CAGCTGTATC TCCAGAAGAT 
GCTCCAAAGC AGATTATTCA CTGGTCTCAA TGCAAAGCAG CAGGAGGAAA TGGGGAAGAC 
TTGGGATTTG AAAAGCATTC TGCTCCTAAA TCAGACCACA TGAAAGAGAA TGGAGTTGGA 
GACCCCGCTA ATCAAAAGAG TAATAAAAAA CTTCAGCCAG ATAATTCAGT ATTCAGTAGA 
AGCCATCTTC AAGCTAGTAG TGGCAGAGGT CCAGCTAAGT TAGGTTACAG CGATCAAAAG 
GACAGGCTAA AGGACATATC CAGAGAGTGT GCTCAGAAAG CTGCTGATAT GAAAAACTTC 
TCATCTTTAC GAAAAGATGC AGACAGAGGA CCAAGAAAAG AGTCTGGGAG ACAAAGAGAC 
TTGATTGGGG AAGATCGTTC CAATGTTAAG TCGGGGTCTC CTCCAGTTGG AAATGGATTT 
AGACACTGTG CTGATCAGCG CATATACAGC AGCCAGGGAA GAGGCTCCTA TAAGCATGAC 
GGTGCACCTC ACCAAGCAAA GCTTTCTGTA CAGGTGCTTG GATCAAATAA AACAGAAGCG 
TTTCCTGCTG GGGAGAAACC AATGATCAGG ACCCGGACTG ATGGTGTCGC TGGCTATGAT 
ACAGACACCA GTCAAGACTC TAGGGACAAA GGAAATAGCA GCAACAGCAG AAGCAGAAGT 
AAAGGTTGGA AACCTATGCG AGAGACACTA AACGTAGACA GCATTTTCAG TGAGACTGAG 
AAAAAACAGC ATAGCCCAAA GCACAAGGTA AATCCGAACA GTAAATCTAA GCATGAAAAG 
GAGCGAAGCT TTAACCATTG GCCAAAAGAG AACCAAATCC AGAAAGGTTT AATGACTATA 
TATGAAGATG AAACAAAACA GGATACAGGA AGTAGAAGTT CACTGGATTC AGAGGGAAAA 
GGGAATGCTG AAAAAAGCAA AGGATTTACA GAGAGAAAAA TCCACAGTGA TAACTGGCAA 
ATTCAGAGGA CAGAATCTGG ATATGAAAGC AGTGATCATA TCAGCAATGG ATCTGCAAAT 
CCGGACTCGC CCATTATTGA AGGAATCAAT CCGGCAGATG TCAAGAATGT TAAAGAAAAT 
GCTTCCTGCA GTGAACAGAA CTTGTCAACC AAAAAAGCTG ATAATGTGTT ACATTCAGTA 
TCCCAGCAGA ACAGAAACTT TTATGACTTG AGGAAAGACC CAATCAATTC AGAAGTTAAC 
TACAGACCTC ATGTTAATTT CCCAACAGAA CTACATTTGC AGGTGCCCAG TCCTCCAGTA 
AAGAGAGCTG AAATGCCTGA GACCAATAGG AAACTTTTCC AATCATCAGC TCTACAGCCA 
GTTCTTAAAG ACATTGTTAA GTCAGAAAGT AGGAACAAGG CAAACGAACA GAATTCTTCA 
GAGTGGCTTA ATCCAGACAA GATTGAGAAG ATGAATGTGT CAGTATATTG TGCTGATGGC 
ATATCCCACC CCTATACAGA AAATGTCTGC GGAAGGAAGC TGATCAACAA TGACTTTCAC 
TCCTCTTCCC AGCCGCAGTC TCATTATGCA AGAACGTTTG GTCCCAAGGT GGGGTTGTCA 
CCTTGTGTGC CACAGAACCT GTCAGAAAGG CCCATGGTGC TTCCCCCTCC CGGTGAGCAT 
GCCGGGCACC CACTCCGAAG GGTGGATGCT CTTTGGGTGC CTGAAGCAGT GTACCAGAAT 
CTTCCTCCCC CTTTACCGCC CAAGAAATAC GCTCTGAATG CTTCGCCAGG ATCAGAAAAT 
AACTCAGCAG CTGATGTAAA ATCGACAGAA ATGTTGCAAA ATAATCCGGT AAGGCAAACA 
GGTACACCTT TAAAATCTGC ATCAGAAGCA AGCATATTTA CAAATGAACA AAGGCTTAAA 
GAGCCATTTC AAGGGAATGA ACTGTTTGTT CATAAACCGG ATTCTCCACC TCGCTTATCA 
GTTAATGACT TTTGGACTGT GTCTGAAAAC TTTTTAAAGA AAGGATCTCG TCATACGGGA 
CCAGGTGCTG GCTATGTGGA TGGAAATGAT AGCGTATCCC TAACGACATA TTTTTCAGTA 
GACAGTTGTA TGACTGACAC ATACAGGATG AAATATCATC AGAGACCCAA GCTGTATTTT 
ACAGATAGTG GAAGTTTTCA CAAAGAAAAA CATCCTCCAT CAGCTGGAGT AGAACTGAGT 
ACTACGTACC ACGCTACTCT TGAGCCCAGG CATCCCACAT CTGAGCAAAG GTACAAGGCA 
AATGTAGAAG GCATACACTG CAGTAGATAG