CHAT cDNA ORF clone, Aquila chrysaetos canadensis

Gene
Clones
gRNAs

Summary

Gene Symbol	CHAT
Entrez Gene ID	105402222
Full Name	choline O-acetyltransferase
Gene Type	protein-coding
Organism	Aquila chrysaetos canadensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_011577257.1	XP_011575559.1	choline O-acetyltransferase

Pathways from BioSystems
Gene Ontology

The following CHAT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHAT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAq16442	XM_011577257.1 Latest version!	Aquila chrysaetos canadensis choline O-acetyltransferase (CHAT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHAT ( XM_011577257.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011575559.1 Aquila chrysaetos canadensis choline O-acetyltransferase (CHAT), mRNA.

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CloneID	OAq16442
Clone ID Related Accession (Same CDS sequence)	XM_011577257.1
Accession Version	XM_011577257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	choline O-acetyltransferase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950889.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTCC TAGAAAAAAA CATGCAGAAG AAAGAGAAAG ATACTTGCAG TAAAGATGAG 
ACAGCAGTGC CAAAACTTCC AGTCCCACCA CTACAACAGA CCTTACGCAT GTACCTACAG 
TGCATGAAAC ACTTGGTGCC AGAGGAGCAA TTTAGAAAGA CCAAGGTCAT TGTGGAGCAG 
TTTGGAATTG CAGGAGGCCT GGGGGAATCT TTGCAGCAAA TCCTCCAGGA AAGAAGGGAA 
AAGACAACGA ACTGGGTGTT TAACTACTGG TTGGATGACA TGTACCTCAA CAACCGGCTA 
GCTCTTCCAG TCAATTCCAG CCCAGCAATT ATCTTTGCTC GTCAGTATTT CAAGGACGTA 
AATGACCAGC TGAGGTTTGC CGCCAATCTC ATTTCAGGAG TGCAAGACTA TAAAGCTTTA 
CTAGACACCC ATGCTTTACC TGTTGATTTT GCTCGTGGAC AGCTGTCTGG TCACCCTCTC 
TGTATGAAGC AATACTATGG ACTCTTTTCT TCCTATCGTC TTCCAGGACA TACCAAAGAT 
ACACTCGTGG CCCAGAAAAG CAGCGTAATG CCAGAACCAG AGCACATCAT TGTTGCTTGT 
AACAATCAGT TTTTTGTTTT GGATGTTGTC ATTAATTTCC GTCGTCTCAG TGAGGGAGAT 
CTTTTCACTC AGTTACGAAA GATAGCCAAA ATGGCAGAGA ACGAAGAGGA AATGCTGCCT 
CCAATTGGCC TGCTGACAAC AGACGGAAGA ACAGAGTGGG CAGAGGCCAG GACGATCCTT 
ATGAAAGACT CTACTAACCG CGACTCTCTT GACATGATTG AACGGTGCAT TTGCCTGGTG 
TGCCTGGACA GCCCAAGCGA GGTGGAACTC AGCGATACAA ACATGGCACT GCAACTGCTA 
CATGGGGGAG GCTACCGTAA AAATGGGGCC AACCGTTGGT ATGACAAACC TATGCAGTTT 
GTGGTAGGAA GAGATGGAGT CTGTGGTACC GTGTGTGAAC ATTCCCCTTT CGATGGCATC 
GTACTGGTGC AGTGCACTGA ACACTTGCTC AAGCACATGA AAGAGAGCTC CAAGAAATTA 
GTCCGAGCAG ATTCAGTGAG TGAGCTTCCT GCTCCACGGA GACTAAGATG GAAATGTTCC 
CCTGAAATTC AGGCGCGTTT GGCATCAGCA GCAGAAAAAC TCCAAAGGAT AGTAAAAAAT 
CTGGACTTCA TCGCCTACAA GTTTGAGAAC TATGGAAAGG AATTTATTAA GAAACAAAAG 
ATTAGCCCAG ATGCCTACAT TCAAGTAGCA CTTCAGCTGG CATTTTACAG ATGCCACAGG 
AGACTTGTCC CTACCTACGA AAGTGCTTCC ATACGCCGAT TTGATGAGGG CAGAGTTGAC 
AATATTAGGT CTGCTACCTC AGAAGCATTT GCTTTTGTAA AAGCAATGAC TGATGACAAG 
ACAGCTCTAT CGGATTCTGA GAAGATGCAG AAATTTAAGG ATGCAATTGC AGCTCAGACT 
AATTACACTA TTCTGGCTAT TACTGGAATG GCAATAGACA ATCACCTGCT AGGGCTCAGG 
GAGGTGGCAC GGGAACACTT CAAGGAACTG CCAGAGATAT TTACGGATGA GACATATTTG 
ACAAGCAATC GATTCATCCT CTCGACCAGC CAGGTTCCAA CTACAACGGA AATGTTCTGC 
TGCTATGGGC CAGTCGTGCC CAATGGCTAT GGGGCATGTT ATAACCCTCA GCCAGAGCAT 
ATATTATTCT GCATCTCAAG CTTTAAAGAC TGCAAAGAAA CCTCCTCAGA CATGTTTGCA 
AAAGCTGTGG AAGAAAGTCT CCTAGAAATG AGAGATCTGT GCAGCAAATG CAACTCTACA 
ATGGCAAAGC CACTTGCTAA ACAAGAAGCA GCCACACAGC TGCAGAGCGA CCGCAAACTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011575559.1
CDS	1..1923
Translation	MPVLEKNMQKKEKDTCSKDETAVPKLPVPPLQQTLRMYLQCMKHLVPEEQFRKTKVIVEQFGIAGGLGESLQQILQERREKTTNWVFNYWLDDMYLNNRLALPVNSSPAIIFARQYFKDVNDQLRFAANLISGVQDYKALLDTHALPVDFARGQLSGHPLCMKQYYGLFSSYRLPGHTKDTLVAQKSSVMPEPEHIIVACNNQFFVLDVVINFRRLSEGDLFTQLRKIAKMAENEEEMLPPIGLLTTDGRTEWAEARTILMKDSTNRDSLDMIERCICLVCLDSPSEVELSDTNMALQLLHGGGYRKNGANRWYDKPMQFVVGRDGVCGTVCEHSPFDGIVLVQCTEHLLKHMKESSKKLVRADSVSELPAPRRLRWKCSPEIQARLASAAEKLQRIVKNLDFIAYKFENYGKEFIKKQKISPDAYIQVALQLAFYRCHRRLVPTYESASIRRFDEGRVDNIRSATSEAFAFVKAMTDDKTALSDSEKMQKFKDAIAAQTNYTILAITGMAIDNHLLGLREVAREHFKELPEIFTDETYLTSNRFILSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPEHILFCISSFKDCKETSSDMFAKAVEESLLEMRDLCSKCNSTMAKPLAKQEAATQLQSDRKL

Target ORF information:

RefSeq Version	XM_011577257.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis choline O-acetyltransferase (CHAT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011577257.1

ORF Insert Sequence:

ATGCCTGTCC TAGAAAAAAA CATGCAGAAG AAAGAGAAAG ATACTTGCAG TAAAGATGAG 
ACAGCAGTGC CAAAACTTCC AGTCCCACCA CTACAACAGA CCTTACGCAT GTACCTACAG 
TGCATGAAAC ACTTGGTGCC AGAGGAGCAA TTTAGAAAGA CCAAGGTCAT TGTGGAGCAG 
TTTGGAATTG CAGGAGGCCT GGGGGAATCT TTGCAGCAAA TCCTCCAGGA AAGAAGGGAA 
AAGACAACGA ACTGGGTGTT TAACTACTGG TTGGATGACA TGTACCTCAA CAACCGGCTA 
GCTCTTCCAG TCAATTCCAG CCCAGCAATT ATCTTTGCTC GTCAGTATTT CAAGGACGTA 
AATGACCAGC TGAGGTTTGC CGCCAATCTC ATTTCAGGAG TGCAAGACTA TAAAGCTTTA 
CTAGACACCC ATGCTTTACC TGTTGATTTT GCTCGTGGAC AGCTGTCTGG TCACCCTCTC 
TGTATGAAGC AATACTATGG ACTCTTTTCT TCCTATCGTC TTCCAGGACA TACCAAAGAT 
ACACTCGTGG CCCAGAAAAG CAGCGTAATG CCAGAACCAG AGCACATCAT TGTTGCTTGT 
AACAATCAGT TTTTTGTTTT GGATGTTGTC ATTAATTTCC GTCGTCTCAG TGAGGGAGAT 
CTTTTCACTC AGTTACGAAA GATAGCCAAA ATGGCAGAGA ACGAAGAGGA AATGCTGCCT 
CCAATTGGCC TGCTGACAAC AGACGGAAGA ACAGAGTGGG CAGAGGCCAG GACGATCCTT 
ATGAAAGACT CTACTAACCG CGACTCTCTT GACATGATTG AACGGTGCAT TTGCCTGGTG 
TGCCTGGACA GCCCAAGCGA GGTGGAACTC AGCGATACAA ACATGGCACT GCAACTGCTA 
CATGGGGGAG GCTACCGTAA AAATGGGGCC AACCGTTGGT ATGACAAACC TATGCAGTTT 
GTGGTAGGAA GAGATGGAGT CTGTGGTACC GTGTGTGAAC ATTCCCCTTT CGATGGCATC 
GTACTGGTGC AGTGCACTGA ACACTTGCTC AAGCACATGA AAGAGAGCTC CAAGAAATTA 
GTCCGAGCAG ATTCAGTGAG TGAGCTTCCT GCTCCACGGA GACTAAGATG GAAATGTTCC 
CCTGAAATTC AGGCGCGTTT GGCATCAGCA GCAGAAAAAC TCCAAAGGAT AGTAAAAAAT 
CTGGACTTCA TCGCCTACAA GTTTGAGAAC TATGGAAAGG AATTTATTAA GAAACAAAAG 
ATTAGCCCAG ATGCCTACAT TCAAGTAGCA CTTCAGCTGG CATTTTACAG ATGCCACAGG 
AGACTTGTCC CTACCTACGA AAGTGCTTCC ATACGCCGAT TTGATGAGGG CAGAGTTGAC 
AATATTAGGT CTGCTACCTC AGAAGCATTT GCTTTTGTAA AAGCAATGAC TGATGACAAG 
ACAGCTCTAT CGGATTCTGA GAAGATGCAG AAATTTAAGG ATGCAATTGC AGCTCAGACT 
AATTACACTA TTCTGGCTAT TACTGGAATG GCAATAGACA ATCACCTGCT AGGGCTCAGG 
GAGGTGGCAC GGGAACACTT CAAGGAACTG CCAGAGATAT TTACGGATGA GACATATTTG 
ACAAGCAATC GATTCATCCT CTCGACCAGC CAGGTTCCAA CTACAACGGA AATGTTCTGC 
TGCTATGGGC CAGTCGTGCC CAATGGCTAT GGGGCATGTT ATAACCCTCA GCCAGAGCAT 
ATATTATTCT GCATCTCAAG CTTTAAAGAC TGCAAAGAAA CCTCCTCAGA CATGTTTGCA 
AAAGCTGTGG AAGAAAGTCT CCTAGAAATG AGAGATCTGT GCAGCAAATG CAACTCTACA 
ATGGCAAAGC CACTTGCTAA ACAAGAAGCA GCCACACAGC TGCAGAGCGA CCGCAAACTC 
TAA