Eaat-2 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eaat-2
Entrez Gene ID	406113
Full Name	excitatory amino acid transporter 2
Synonyms	Eaat,GB16377
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006568148.3	XP_006568211.1	excitatory amino acid transporter 2 isoform X1
XM_006568148.2	XP_006568211.1	excitatory amino acid transporter 2 isoform X1
NM_001011597.1	NP_001011597.1	excitatory amino acid transporter 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
Elsik CG, Worley KC, Bennett AK, Beye M, Camara F, Childers CP, de Graaf DC, Debyser G, Deng J, Devreese B, Elhaik E, Evans JD, Foster LJ, Graur D, Guigo R, , Hoff KJ, Holder ME, Hudson ME, Hunt GJ, Jiang H, Joshi V, Khetani RS, Kosarev P, Kovar CL, Ma J, Maleszka R, Moritz RF, Munoz-Torres MC, Murphy TD, Muzny DM, Newsham IF, Reese JT, Robertson HM, Robinson GE, Rueppell O, Solovyev V, Stanke M, Stolle E, Tsuruda JM, Vaerenbergh MV, Waterhouse RM, Weaver DB, Whitfield CW, Wu Y, Zdobnov EM, Zhang L, Zhu D, Gibbs RA,
BMC genomics1586(2014 Jan)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

Molecular cloning and expression analysis of a cDNA encoding a glutamate transporter in the honeybee brain.
Kucharski R, Ball EE, Hayward DC, Maleszka R
Gene242(1-2)399-405(2000 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Eaat-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eaat-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHd00097	NM_001011597.1 Latest version!	Apis mellifera excitatory amino acid transporter 2 (Eaat-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OHd03202	XM_006568148.2 Latest version!	Apis mellifera excitatory amino acid transporter 2 (Eaat-2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OHd28407	XM_006568148.3 Latest version!	Apis mellifera excitatory amino acid transporter 2 (Eaat-2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eaat-2 ( NM_001011597.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001011597.1 Apis mellifera excitatory amino acid transporter 2 (Eaat-2), mRNA.

Price & Availability↑

CloneID	OHd00097
Clone ID Related Accession (Same CDS sequence)	NM_001011597.1
Accession Version	NM_001011597.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Apis mellifera(honey bee)
Product	excitatory amino acid transporter 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF144379.1. On Feb 5, 2005 this sequence version replaced XM_392906.1. ##Evidence-Data-START## Transcript exon combination :: AF144379.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02717013, SAMN02728803 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCTCG GGCAACGTAT ATCTGGGGCC GTCGCGGTCC TCAGAGGCAC TTCCGAAGTC 
AAAGAAGCCC AAACGTCAAC CTCCGATGAG ATCGAAAAGA TAACCACTGT GGAGGAGGAA 
AAAATTTGCC ACAAGGCAAT CACCCCGCTC GACAGATTGC AACGAGTGGT GGATTGGTTC 
AGGAAGAACA TGCTTTTGGT CTTTACGATC GCCGCAGTCT TGGTCGGTCT TATTCTTGGA 
TTTCTCGGCC GATTAGCCAA TCCCACGCCA CAATCCATTA CTCTTATCAG TTTCCCCGGA 
GAACTTCTTA TGCGACTTTT AAAGATGTTC ATACTACCGC TCATTGTCTC GTCTCTTATC 
TCCGGAATGG CACAATTGGA CCCTAAGGGT TCAGGAAGAA TGGGATACAG AGCATTGTTA 
TATTACACCG TGACGACCAT TCTCGCGGCT ATAGTTGGTA TCGTAATGGT ATTAATTATC 
CATCCCGGTG ATCCTAGAAT TAAATCTGTT GTAACGGCGT ACAAGGCGGA TTATACAAAA 
ATTTCTACGT TAGACGCTAT CCTCGATATA ATTAGAAACA TGGTGCCGGA AAATTTGGTG 
CAGGCGTGCT TCCAGCAGGC GCAAACTACT TACGTGACGA AGGAAGTTGC TACGGGAACT 
GCAAGTGAAA TTACTCAAAT ACAAGAGGCA ACGTTAGTGT ACAAGGATGG GACGAATGTG 
ATGGGCATGA TAGTGTTTTG CATCACGTTT GGACTTGTTG CTGGACAATT TGGCGCCCAA 
GGGAAATTAA TCGTCGACTT CTTCATGATT TTGAACGAAA TAATCATGAA ACTTGTCGGT 
ATTATTATCA TGTGGTATTC TCCGTTCGGA ATTATGTGTC TGATAGCTGG TAAAATAATG 
TCGATCAACA ATTTGACGGC AACTGCTCAG ATGTTAGGAC TGTACATGGT GACAGTCATA 
TTGGGCTTAT TGTTCCACGC ATTAATCACT CTGCCAACGA TATTTTGGTT TTTAACTCGA 
CAGAATCCTG CCGCATTTTT CAGGGGTATG ATGCAAGCTT GGATGACAGC GTTAGGAACG 
GCATCAAGCG CCGCAACTTT ACCAATAACT TTTCGATGCC TCGAGGAAAA TAATAAAATC 
GATTCACGTG TAACGCGATT CGTAGTAGCG GTTGGGGCTA CGGTAAATAT GGATGGAACG 
GCTCTTTACG AAGCGGTTGC CGCGATTTTT ATCGCACAAA TGAATGGAAT TTCGTTAGGA 
ATAGGTGAAG TTATAACGGT CAGTTTCACA GCTACCTTGG CAAGTATAGG TGCAGCAAGT 
ATTCCCAGCG CGGCTTTGAT TACGATGTTA ATAGTGCTTA CAGCTTTGGG TTTACCAACC 
AACGATATTT CTCTATTGTT TGCTGTTGAT TGGATGTTGG ATCGAATCAG GACCTCGATC 
AATGTTTTGG GCGATGGATA CGGGGCTGGA ATAGTTTATC ATTTGAGCAA AGCTGAATTG 
GATAAAATGG ATAAAGAAAA GAAATTAGAG GATCTTGAAA TGGGGACACC TCTTGAAAAT 
ATTTTGGAGA GCTGTCAGCA AGATGAAACG CATGCTCCCG AGAAAACAAA GATTTTCCTT 
TCCGAAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011597.1
CDS	378..2009
Translation	MSLGQRISGAVAVLRGTSEVKEAQTSTSDEIEKITTVEEEKICHKAITPLDRLQRVVDWFRKNMLLVFTIAAVLVGLILGFLGRLANPTPQSITLISFPGELLMRLLKMFILPLIVSSLISGMAQLDPKGSGRMGYRALLYYTVTTILAAIVGIVMVLIIHPGDPRIKSVVTAYKADYTKISTLDAILDIIRNMVPENLVQACFQQAQTTYVTKEVATGTASEITQIQEATLVYKDGTNVMGMIVFCITFGLVAGQFGAQGKLIVDFFMILNEIIMKLVGIIIMWYSPFGIMCLIAGKIMSINNLTATAQMLGLYMVTVILGLLFHALITLPTIFWFLTRQNPAAFFRGMMQAWMTALGTASSAATLPITFRCLEENNKIDSRVTRFVVAVGATVNMDGTALYEAVAAIFIAQMNGISLGIGEVITVSFTATLASIGAASIPSAALITMLIVLTALGLPTNDISLLFAVDWMLDRIRTSINVLGDGYGAGIVYHLSKAELDKMDKEKKLEDLEMGTPLENILESCQQDETHAPEKTKIFLSET

Target ORF information:

RefSeq Version	NM_001011597.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera excitatory amino acid transporter 2 (Eaat-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011597.1

ORF Insert Sequence:

ATGTCTCTCG GGCAACGTAT ATCTGGGGCC GTCGCGGTCC TCAGAGGCAC TTCCGAAGTC 
AAAGAAGCCC AAACGTCAAC CTCCGATGAG ATCGAAAAGA TAACCACTGT GGAGGAGGAA 
AAAATTTGCC ACAAGGCAAT CACCCCGCTC GACAGATTGC AACGAGTGGT GGATTGGTTC 
AGGAAGAACA TGCTTTTGGT CTTTACGATC GCCGCAGTCT TGGTCGGTCT TATTCTTGGA 
TTTCTCGGCC GATTAGCCAA TCCCACGCCA CAATCCATTA CTCTTATCAG TTTCCCCGGA 
GAACTTCTTA TGCGACTTTT AAAGATGTTC ATACTACCGC TCATTGTCTC GTCTCTTATC 
TCCGGAATGG CACAATTGGA CCCTAAGGGT TCAGGAAGAA TGGGATACAG AGCATTGTTA 
TATTACACCG TGACGACCAT TCTCGCGGCT ATAGTTGGTA TCGTAATGGT ATTAATTATC 
CATCCCGGTG ATCCTAGAAT TAAATCTGTT GTAACGGCGT ACAAGGCGGA TTATACAAAA 
ATTTCTACGT TAGACGCTAT CCTCGATATA ATTAGAAACA TGGTGCCGGA AAATTTGGTG 
CAGGCGTGCT TCCAGCAGGC GCAAACTACT TACGTGACGA AGGAAGTTGC TACGGGAACT 
GCAAGTGAAA TTACTCAAAT ACAAGAGGCA ACGTTAGTGT ACAAGGATGG GACGAATGTG 
ATGGGCATGA TAGTGTTTTG CATCACGTTT GGACTTGTTG CTGGACAATT TGGCGCCCAA 
GGGAAATTAA TCGTCGACTT CTTCATGATT TTGAACGAAA TAATCATGAA ACTTGTCGGT 
ATTATTATCA TGTGGTATTC TCCGTTCGGA ATTATGTGTC TGATAGCTGG TAAAATAATG 
TCGATCAACA ATTTGACGGC AACTGCTCAG ATGTTAGGAC TGTACATGGT GACAGTCATA 
TTGGGCTTAT TGTTCCACGC ATTAATCACT CTGCCAACGA TATTTTGGTT TTTAACTCGA 
CAGAATCCTG CCGCATTTTT CAGGGGTATG ATGCAAGCTT GGATGACAGC GTTAGGAACG 
GCATCAAGCG CCGCAACTTT ACCAATAACT TTTCGATGCC TCGAGGAAAA TAATAAAATC 
GATTCACGTG TAACGCGATT CGTAGTAGCG GTTGGGGCTA CGGTAAATAT GGATGGAACG 
GCTCTTTACG AAGCGGTTGC CGCGATTTTT ATCGCACAAA TGAATGGAAT TTCGTTAGGA 
ATAGGTGAAG TTATAACGGT CAGTTTCACA GCTACCTTGG CAAGTATAGG TGCAGCAAGT 
ATTCCCAGCG CGGCTTTGAT TACGATGTTA ATAGTGCTTA CAGCTTTGGG TTTACCAACC 
AACGATATTT CTCTATTGTT TGCTGTTGAT TGGATGTTGG ATCGAATCAG GACCTCGATC 
AATGTTTTGG GCGATGGATA CGGGGCTGGA ATAGTTTATC ATTTGAGCAA AGCTGAATTG 
GATAAAATGG ATAAAGAAAA GAAATTAGAG GATCTTGAAA TGGGGACACC TCTTGAAAAT 
ATTTTGGAGA GCTGTCAGCA AGATGAAACG CATGCTCCCG AGAAAACAAA GATTTTCCTT 
TCCGAAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eaat-2 ( XM_006568148.2 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_006568211.1 Apis mellifera excitatory amino acid transporter 2 (Eaat-2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHd03202
Clone ID Related Accession (Same CDS sequence)	XM_006568148.2
Accession Version	XM_006568148.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-23
Organism	Apis mellifera(honey bee)
Product	excitatory amino acid transporter 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007071.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 24, 2016 this sequence version replaced XM_006568148.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCTCTCG GGCAACGTAT ATCTGGGGCC GTCGCGGTCC TCAGAGGCAC TTCCGAAGTC 
AAAGAAGCCC AAACGTCAAC CTCCGATGAG ATCGAAAAGA TAACCACTGT GGAGGAGGAA 
AAAATTTGCC ACAAGGCAAT CACCCCGCTC GACAGATTGC AACGAGTGGT GGATTGGTTC 
AGGAAGAACA TGCTTTTGGT CTTTACGATC GCCGCAGTCT TGGTCGGTCT TATTCTTGGA 
TTTCTCGGCC GATTAGCCAA TCCCACGCCA CAATCCATTA CTCTTATCAG TTTCCCCGGA 
GAACTTCTTA TGCGACTTTT AAAGATGTTC ATACTACCGC TCATTGTCTC GTCTCTTATC 
TCCGGAATGG CACAATTGGA CCCTAAGGGT TCAGGAAGAA TGGGATACAG AGCATTGTTA 
TATTACACCG TGACGACCAT TCTCGCGGCT ATAGTTGGTA TCGTAATGGT ATTAATTATC 
CATCCCGGTG ATCCTAGAAT TAAATCTGTT GTAACGGCGT ACAAGGCGGA CTATACAAAA 
ATTTCTACGT TAGACGCTAT CCTCGATATA ATTAGAAACA TGGTGCCGGA AAATTTGGTG 
CAGGCGTGCT TCCAGCAGGC GCAAACTACT TACGTGACGA AGGAAGTTGC TACGGGAACT 
GCAAGTGAAA TTACTCAAAT ACAAGAGGCA ACGTTAGTGT ACAAGGATGG GACGAATGTG 
ATGGGCATGA TAGTGTTTTG CATCACGTTT GGACTTGTTG CTGGACAATT TGGCGCCCAA 
GGGAAATTAA TCGTCGACTT CTTCATGATT TTGAACGAAA TAATCATGAA ACTTGTCGGT 
ATTATTATCA TGTGGTATTC TCCGTTCGGA ATTATGTGTC TGATAGCTGG TAAAATAATG 
TCGATCAACA ATTTGACGGC AACTGCTCAG ATGTTAGGAC TGTACATGGT GACAGTCATA 
TTGGGCTTAT TGTTCCACGC ATTAATCACT CTGCCAACGA TATTTTGGTT TTTAACTCGA 
CAGAATCCTG CCGCATTTTT CAGAGGTATG ATGCAAGCTT GGATGACAGC GTTAGGAACG 
GCATCAAGCG CCGCAACTTT ACCAATAACT TTTCGATGCC TCGAGGAAAA TAATAAAATC 
GATTCACGTG TAACGCGATT CGTAGTAGCG GTTGGGGCTA CGGTAAATAT GGATGGAACG 
GCTCTTTACG AAGCGGTTGC CGCGATTTTT ATCGCACAAA TGAATGGAAT TTCGTTAGGA 
ATAGGTGAAG TTATAACGGT CAGTTTCACA GCTACCTTGG CAAGTATAGG TGCAGCAAGT 
ATTCCCAGCG CGGCTTTGAT TACGATGTTA ATAGTGCTTA CAGCTTTGGG TTTACCAACG 
AACGATATTT CTCTATTGTT TGCTGTTGAT TGGATGTTGG ATCGAATCAG GACCTCGATC 
AATGTTTTGG GCGATGGATA CGGGGCTGGA ATAGTTTATC ATTTGAGCAA AGCTGAATTG 
GATAAAATGG ATAAAGAAAA GAAATTAGAG GATCTTGAAA TGGGGACACC TCTTGAAAAT 
ATTTTGGAGA GCTGTCAGCA AGATGAAACG CATGCTCCCG AGAAAACAAA GATTTTCCTT 
TCCGAAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006568211.1
CDS	298..1929
Translation	MSLGQRISGAVAVLRGTSEVKEAQTSTSDEIEKITTVEEEKICHKAITPLDRLQRVVDWFRKNMLLVFTIAAVLVGLILGFLGRLANPTPQSITLISFPGELLMRLLKMFILPLIVSSLISGMAQLDPKGSGRMGYRALLYYTVTTILAAIVGIVMVLIIHPGDPRIKSVVTAYKADYTKISTLDAILDIIRNMVPENLVQACFQQAQTTYVTKEVATGTASEITQIQEATLVYKDGTNVMGMIVFCITFGLVAGQFGAQGKLIVDFFMILNEIIMKLVGIIIMWYSPFGIMCLIAGKIMSINNLTATAQMLGLYMVTVILGLLFHALITLPTIFWFLTRQNPAAFFRGMMQAWMTALGTASSAATLPITFRCLEENNKIDSRVTRFVVAVGATVNMDGTALYEAVAAIFIAQMNGISLGIGEVITVSFTATLASIGAASIPSAALITMLIVLTALGLPTNDISLLFAVDWMLDRIRTSINVLGDGYGAGIVYHLSKAELDKMDKEKKLEDLEMGTPLENILESCQQDETHAPEKTKIFLSET

Target ORF information:

RefSeq Version	XM_006568148.2
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera excitatory amino acid transporter 2 (Eaat-2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006568148.2

ORF Insert Sequence:

ATGTCTCTCG GGCAACGTAT ATCTGGGGCC GTCGCGGTCC TCAGAGGCAC TTCCGAAGTC 
AAAGAAGCCC AAACGTCAAC CTCCGATGAG ATCGAAAAGA TAACCACTGT GGAGGAGGAA 
AAAATTTGCC ACAAGGCAAT CACCCCGCTC GACAGATTGC AACGAGTGGT GGATTGGTTC 
AGGAAGAACA TGCTTTTGGT CTTTACGATC GCCGCAGTCT TGGTCGGTCT TATTCTTGGA 
TTTCTCGGCC GATTAGCCAA TCCCACGCCA CAATCCATTA CTCTTATCAG TTTCCCCGGA 
GAACTTCTTA TGCGACTTTT AAAGATGTTC ATACTACCGC TCATTGTCTC GTCTCTTATC 
TCCGGAATGG CACAATTGGA CCCTAAGGGT TCAGGAAGAA TGGGATACAG AGCATTGTTA 
TATTACACCG TGACGACCAT TCTCGCGGCT ATAGTTGGTA TCGTAATGGT ATTAATTATC 
CATCCCGGTG ATCCTAGAAT TAAATCTGTT GTAACGGCGT ACAAGGCGGA CTATACAAAA 
ATTTCTACGT TAGACGCTAT CCTCGATATA ATTAGAAACA TGGTGCCGGA AAATTTGGTG 
CAGGCGTGCT TCCAGCAGGC GCAAACTACT TACGTGACGA AGGAAGTTGC TACGGGAACT 
GCAAGTGAAA TTACTCAAAT ACAAGAGGCA ACGTTAGTGT ACAAGGATGG GACGAATGTG 
ATGGGCATGA TAGTGTTTTG CATCACGTTT GGACTTGTTG CTGGACAATT TGGCGCCCAA 
GGGAAATTAA TCGTCGACTT CTTCATGATT TTGAACGAAA TAATCATGAA ACTTGTCGGT 
ATTATTATCA TGTGGTATTC TCCGTTCGGA ATTATGTGTC TGATAGCTGG TAAAATAATG 
TCGATCAACA ATTTGACGGC AACTGCTCAG ATGTTAGGAC TGTACATGGT GACAGTCATA 
TTGGGCTTAT TGTTCCACGC ATTAATCACT CTGCCAACGA TATTTTGGTT TTTAACTCGA 
CAGAATCCTG CCGCATTTTT CAGAGGTATG ATGCAAGCTT GGATGACAGC GTTAGGAACG 
GCATCAAGCG CCGCAACTTT ACCAATAACT TTTCGATGCC TCGAGGAAAA TAATAAAATC 
GATTCACGTG TAACGCGATT CGTAGTAGCG GTTGGGGCTA CGGTAAATAT GGATGGAACG 
GCTCTTTACG AAGCGGTTGC CGCGATTTTT ATCGCACAAA TGAATGGAAT TTCGTTAGGA 
ATAGGTGAAG TTATAACGGT CAGTTTCACA GCTACCTTGG CAAGTATAGG TGCAGCAAGT 
ATTCCCAGCG CGGCTTTGAT TACGATGTTA ATAGTGCTTA CAGCTTTGGG TTTACCAACG 
AACGATATTT CTCTATTGTT TGCTGTTGAT TGGATGTTGG ATCGAATCAG GACCTCGATC 
AATGTTTTGG GCGATGGATA CGGGGCTGGA ATAGTTTATC ATTTGAGCAA AGCTGAATTG 
GATAAAATGG ATAAAGAAAA GAAATTAGAG GATCTTGAAA TGGGGACACC TCTTGAAAAT 
ATTTTGGAGA GCTGTCAGCA AGATGAAACG CATGCTCCCG AGAAAACAAA GATTTTCCTT 
TCCGAAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eaat-2 ( XM_006568148.3 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_006568211.1 Apis mellifera excitatory amino acid transporter 2 (Eaat-2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHd28407
Clone ID Related Accession (Same CDS sequence)	XM_006568148.3
Accession Version	XM_006568148.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-18
Organism	Apis mellifera(honey bee)
Product	excitatory amino acid transporter 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037639.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 19, 2018 this sequence version replaced XM_006568148.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTCG GGCAACGTAT ATCTGGGGCC GTCGCGGTCC TCAGAGGCAC TTCCGAAGTC 
AAAGAAGCCC AAACGTCAAC CTCCGATGAG ATCGAAAAGA TAACCACTGT GGAGGAGGAA 
AAAATTTGCC ACAAGGCAAT CACCCCGCTC GACAGATTGC AACGAGTGGT GGATTGGTTC 
AGGAAGAACA TGCTTTTGGT CTTTACGATC GCCGCAGTCT TGGTCGGTCT TATTCTTGGA 
TTTCTCGGCC GATTAGCCAA TCCCACGCCA CAATCCATTA CTCTTATCAG TTTCCCCGGA 
GAACTTCTTA TGCGACTTTT AAAGATGTTC ATACTACCGC TCATTGTCTC GTCTCTTATC 
TCCGGAATGG CACAATTGGA CCCTAAGGGT TCAGGAAGAA TGGGATACAG AGCATTGTTA 
TATTACACCG TGACGACCAT TCTCGCGGCT ATAGTTGGTA TCGTAATGGT ATTAATTATC 
CATCCCGGTG ATCCTAGAAT TAAATCTGTT GTAACGGCGT ACAAGGCGGA TTATACAAAA 
ATTTCTACGT TAGACGCTAT CCTCGATATA ATTAGAAACA TGGTGCCGGA AAATTTGGTG 
CAGGCGTGCT TCCAGCAGGC GCAAACTACT TACGTGACGA AGGAAGTTGC TACGGGAACT 
GCAAGTGAAA TTACTCAAAT ACAAGAGGCA ACGTTAGTGT ACAAGGATGG GACGAATGTG 
ATGGGCATGA TAGTGTTTTG CATCACGTTT GGACTTGTTG CTGGACAATT TGGCGCCCAA 
GGGAAATTAA TCGTCGACTT CTTCATGATT TTGAACGAAA TAATCATGAA ACTTGTCGGT 
ATTATTATCA TGTGGTATTC TCCGTTCGGA ATTATGTGTC TGATAGCTGG TAAAATAATG 
TCGATCAACA ATTTGACGGC AACTGCTCAG ATGTTAGGCC TGTACATGGT GACAGTCATA 
TTGGGCTTAT TGTTCCACGC ATTAATCACT CTGCCAACGA TATTTTGGTT TTTAACTCGA 
CAGAATCCTG CCGCATTTTT CAGAGGTATG ATGCAAGCTT GGATGACAGC GTTAGGAACG 
GCATCAAGCG CCGCAACTTT ACCAATAACT TTTCGATGCC TCGAGGAAAA TAATAAAATC 
GATTCACGTG TAACGCGATT CGTAGTAGCG GTTGGGGCTA CGGTAAATAT GGATGGAACG 
GCTCTTTACG AAGCGGTTGC CGCGATTTTT ATCGCACAAA TGAATGGAAT TTCGTTAGGA 
ATAGGTGAAG TTATAACGGT CAGTTTCACA GCTACCTTGG CAAGTATAGG TGCAGCAAGT 
ATTCCCAGCG CGGCTTTGAT TACGATGTTA ATAGTGCTTA CAGCTTTGGG TTTACCAACG 
AACGATATTT CTCTATTGTT TGCTGTTGAT TGGATGTTGG ATCGAATCAG GACCTCGATC 
AATGTTTTGG GCGATGGATA CGGGGCTGGA ATAGTTTATC ATTTGAGCAA AGCTGAATTG 
GATAAAATGG ATAAAGAAAA GAAATTAGAG GATCTTGAAA TGGGGACACC TCTTGAAAAT 
ATTTTGGAGA GCTGTCAGCA AGATGAAACG CATGCTCCCG AGAAAACAAA GATTTTCCTT 
TCCGAAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006568211.1
CDS	247..1878
Translation	MSLGQRISGAVAVLRGTSEVKEAQTSTSDEIEKITTVEEEKICHKAITPLDRLQRVVDWFRKNMLLVFTIAAVLVGLILGFLGRLANPTPQSITLISFPGELLMRLLKMFILPLIVSSLISGMAQLDPKGSGRMGYRALLYYTVTTILAAIVGIVMVLIIHPGDPRIKSVVTAYKADYTKISTLDAILDIIRNMVPENLVQACFQQAQTTYVTKEVATGTASEITQIQEATLVYKDGTNVMGMIVFCITFGLVAGQFGAQGKLIVDFFMILNEIIMKLVGIIIMWYSPFGIMCLIAGKIMSINNLTATAQMLGLYMVTVILGLLFHALITLPTIFWFLTRQNPAAFFRGMMQAWMTALGTASSAATLPITFRCLEENNKIDSRVTRFVVAVGATVNMDGTALYEAVAAIFIAQMNGISLGIGEVITVSFTATLASIGAASIPSAALITMLIVLTALGLPTNDISLLFAVDWMLDRIRTSINVLGDGYGAGIVYHLSKAELDKMDKEKKLEDLEMGTPLENILESCQQDETHAPEKTKIFLSET

Target ORF information:

RefSeq Version	XM_006568148.3
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera excitatory amino acid transporter 2 (Eaat-2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006568148.3

ORF Insert Sequence:

ATGTCTCTCG GGCAACGTAT ATCTGGGGCC GTCGCGGTCC TCAGAGGCAC TTCCGAAGTC 
AAAGAAGCCC AAACGTCAAC CTCCGATGAG ATCGAAAAGA TAACCACTGT GGAGGAGGAA 
AAAATTTGCC ACAAGGCAAT CACCCCGCTC GACAGATTGC AACGAGTGGT GGATTGGTTC 
AGGAAGAACA TGCTTTTGGT CTTTACGATC GCCGCAGTCT TGGTCGGTCT TATTCTTGGA 
TTTCTCGGCC GATTAGCCAA TCCCACGCCA CAATCCATTA CTCTTATCAG TTTCCCCGGA 
GAACTTCTTA TGCGACTTTT AAAGATGTTC ATACTACCGC TCATTGTCTC GTCTCTTATC 
TCCGGAATGG CACAATTGGA CCCTAAGGGT TCAGGAAGAA TGGGATACAG AGCATTGTTA 
TATTACACCG TGACGACCAT TCTCGCGGCT ATAGTTGGTA TCGTAATGGT ATTAATTATC 
CATCCCGGTG ATCCTAGAAT TAAATCTGTT GTAACGGCGT ACAAGGCGGA TTATACAAAA 
ATTTCTACGT TAGACGCTAT CCTCGATATA ATTAGAAACA TGGTGCCGGA AAATTTGGTG 
CAGGCGTGCT TCCAGCAGGC GCAAACTACT TACGTGACGA AGGAAGTTGC TACGGGAACT 
GCAAGTGAAA TTACTCAAAT ACAAGAGGCA ACGTTAGTGT ACAAGGATGG GACGAATGTG 
ATGGGCATGA TAGTGTTTTG CATCACGTTT GGACTTGTTG CTGGACAATT TGGCGCCCAA 
GGGAAATTAA TCGTCGACTT CTTCATGATT TTGAACGAAA TAATCATGAA ACTTGTCGGT 
ATTATTATCA TGTGGTATTC TCCGTTCGGA ATTATGTGTC TGATAGCTGG TAAAATAATG 
TCGATCAACA ATTTGACGGC AACTGCTCAG ATGTTAGGCC TGTACATGGT GACAGTCATA 
TTGGGCTTAT TGTTCCACGC ATTAATCACT CTGCCAACGA TATTTTGGTT TTTAACTCGA 
CAGAATCCTG CCGCATTTTT CAGAGGTATG ATGCAAGCTT GGATGACAGC GTTAGGAACG 
GCATCAAGCG CCGCAACTTT ACCAATAACT TTTCGATGCC TCGAGGAAAA TAATAAAATC 
GATTCACGTG TAACGCGATT CGTAGTAGCG GTTGGGGCTA CGGTAAATAT GGATGGAACG 
GCTCTTTACG AAGCGGTTGC CGCGATTTTT ATCGCACAAA TGAATGGAAT TTCGTTAGGA 
ATAGGTGAAG TTATAACGGT CAGTTTCACA GCTACCTTGG CAAGTATAGG TGCAGCAAGT 
ATTCCCAGCG CGGCTTTGAT TACGATGTTA ATAGTGCTTA CAGCTTTGGG TTTACCAACG 
AACGATATTT CTCTATTGTT TGCTGTTGAT TGGATGTTGG ATCGAATCAG GACCTCGATC 
AATGTTTTGG GCGATGGATA CGGGGCTGGA ATAGTTTATC ATTTGAGCAA AGCTGAATTG 
GATAAAATGG ATAAAGAAAA GAAATTAGAG GATCTTGAAA TGGGGACACC TCTTGAAAAT 
ATTTTGGAGA GCTGTCAGCA AGATGAAACG CATGCTCCCG AGAAAACAAA GATTTTCCTT 
TCCGAAACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
BMC genomics1586(2014 Jan)
Elsik CG,Worley KC,Bennett AK,Beye M,Camara F,Childers CP,de Graaf DC,Debyser G,Deng J,Devreese B,Elhaik E,Evans JD,Foster LJ,Graur D,Guigo R,,Hoff KJ,Holder ME,Hudson ME,Hunt GJ,Jiang H,Joshi V,Khetani RS,Kosarev P,Kovar CL,Ma J,Maleszka R,Moritz RF,Munoz-Torres MC,Murphy TD,Muzny DM,Newsham IF,Reese JT,Robertson HM,Robinson GE,Rueppell O,Solovyev V,Stanke M,Stolle E,Tsuruda JM,Vaerenbergh MV,Waterhouse RM,Weaver DB,Whitfield CW,Wu Y,Zdobnov EM,Zhang L,Zhu D,Gibbs RA,

Insights into social insects from the genome of the honeybee Apis mellifera.
Nature443(7114)931-49(2006 Oct)

Molecular cloning and expression analysis of a cDNA encoding a glutamate transporter in the honeybee brain.
Gene242(1-2)399-405(2000 Jan)
Kucharski R,Ball EE,Hayward DC,Maleszka R