ift74 cDNA ORF clone, Anolis carolinensis(green anole)

Gene
Clones
gRNAs

Summary

Gene Symbol	ift74
Entrez Gene ID	100561874
Full Name	intraflagellar transport 74
Gene Type	protein-coding
Organism	Anolis carolinensis(green anole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008103461.2	XP_008101668.1	intraflagellar transport protein 74 homolog

Pathways from BioSystems
Gene Ontology

The following ift74 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ift74 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAn27523	XM_008103461.2 Latest version!	Anolis carolinensis intraflagellar transport 74 (ift74), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ift74 ( XM_008103461.2 ) cDNA ORF clone, Anolis carolinensis(green anole) -> XP_008101668.1 Anolis carolinensis intraflagellar transport 74 (ift74), mRNA.

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CloneID	OAn27523
Clone ID Related Accession (Same CDS sequence)	XM_008103461.2
Accession Version	XM_008103461.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-30
Organism	Anolis carolinensis(green anole)
Product	intraflagellar transport protein 74 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_014777.1) and transcript sequence (GAED01057273.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 31, 2016 this sequence version replaced XM_008103461.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Anolis carolinensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 54 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCAAGCA ATCACAAACC TCCTACTGCA CGCCCGTCTT CAAGAGCTGC AATGGGGCTT 
GGAACGCGAC CAACTTCATC TTCATTACGA CCTCCATCTG CAGCTTTACT TGGTGGAGGA 
ATCCCTCCAG GCACCGCAAG GCCTGGTACA CGGGGTGGAT CCATAGGAAC TGGGGTGGTT 
TTGTCTTCTC AAATTAAAGT TGCAGATCGT CCGGTGACAC AGCAAGGACT CAGTGGAATG 
AAAACTGGAA TCAAAGGGCC TCAGAGACAA GTTATGGATA AATCCTATTA TCTGGGCGTG 
CTTAGAAGCA AAATAAGTGA GCTTACTACA GAAATTAACA AGCTTCAGAA AGAAAGGGAC 
ATGTATAATC AGGAGAATTC TGTCTATCTC TCCTATGAAA AAAGAGCAGA ATCTTTAGCT 
GGTGAGATCA AAGACTTTCA GGGACAACTT GCAGACTACA ATATGCTGGT GGATAAACTT 
AACACCAATA CCGATATGGA AGAAGTAATG AGTGACTATA ATTTGCTGAA AGCACAAAAT 
GATCGTGAAG CCCAAGGAAT CGACATTATA TTTACAGAAA GACAAAACAA AGAAAAGCTC 
ATTCAGGCTG TGGAAGATGA CATCCAGCAG GAAAAGCAGG CAGCAGAAAA CATCATCAAA 
AATATGTCTG AAGAAGATCA AGTGAAATAC ATGGAGACAA AAATGGCAAA TGAAAAACTT 
TTACAGGAAT TATCTGCTTT ACAGCAAGAA CTGGATGCTT TGAATATGAA AGAACAAGCG 
CTAGAAGGAG AAATAGCACA TTCCCAGGTT AAACAAGAGG CTCTACAGCT GTATGAAAAG 
CTTCATGGTC TTGAAGAACA TCGAAATCAA ATGATTTTGG AAGATAAAAA TATGGGATCT 
CCCCAAGAAG AGAGAGAGAG ATTATTAAAA CAGGTGAAGG AAGACAATCA AGAAATAGCA 
AGCATGGAAA GACAGTTGAC CGAGATAAAA GAAAAGATTG ATCAAATCAA TGAAGGTATT 
CGTCAGGTTG ACATGGATCT GGAAGAGCAA CAAGGAGAGA AAAATGTGAA ATACAAAGAG 
TTAAAGAAGA GAGAGGAAAG CATGGATAAC TTTCTTGAAT CTTTTGAAGA GACAAAAAAC 
CAGGAACTGG AACGGAAAAC ACAGATAGAG GCCAATATTG TGGCAATTTT AGAGCACTCA 
AGCAGGAATG TAAATCGTAT GAAGCAGATA TCTTCTGTTA CAAATCAAGA GCTGAAGATC 
ATGCAGGAAG ACCTCACATT CAAATCAAGT GAAATGCAAA AATCACAGAG TACAGCAAAA 
AACCTAGGTT CAGACTGTCA ACGTCTGCAG ATGGATCTCC AGAAGATGGG TCTATTGGAA 
GGCAAGATGA AGGACGAACT CGCTTCTCTC AAAGAAAAAA CTGAGCAAAT GACCAAGGAC 
CTAGAACTGT ACAACAACCT GCCAGCTCTG AAAGCAGCAG GAGAAGAGAG AAAGAACAAA 
CTGCAAGAGG AGAAAGAAAC CCTGACAAAA CACAGGAATG CTTTTAAGAA AATTATGGAG 
GAGATGAATA CGGAGTATGA AGGCCTGAAG CTGCAGCTAC AGGAGAATGA GACGCATGCG 
CAGCTTACAA ATCTGGAAAG GAAGTGGCAG CATCTTGAAC AAAATAATTT TGTCATGAAA 
GAATTTATAG CAACAAAAAC CCAAGAAAGT GATTACCGTC CAGTTGTGAA GAATGTCACA 
AAACAGATTG CAGAATATAA CAAAACCCTC ATAGAAGCTT TAGAGAACAG GAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008101668.1
CDS	209..2005
Translation	MASNHKPPTARPSSRAAMGLGTRPTSSSLRPPSAALLGGGIPPGTARPGTRGGSIGTGVVLSSQIKVADRPVTQQGLSGMKTGIKGPQRQVMDKSYYLGVLRSKISELTTEINKLQKERDMYNQENSVYLSYEKRAESLAGEIKDFQGQLADYNMLVDKLNTNTDMEEVMSDYNLLKAQNDREAQGIDIIFTERQNKEKLIQAVEDDIQQEKQAAENIIKNMSEEDQVKYMETKMANEKLLQELSALQQELDALNMKEQALEGEIAHSQVKQEALQLYEKLHGLEEHRNQMILEDKNMGSPQEERERLLKQVKEDNQEIASMERQLTEIKEKIDQINEGIRQVDMDLEEQQGEKNVKYKELKKREESMDNFLESFEETKNQELERKTQIEANIVAILEHSSRNVNRMKQISSVTNQELKIMQEDLTFKSSEMQKSQSTAKNLGSDCQRLQMDLQKMGLLEGKMKDELASLKEKTEQMTKDLELYNNLPALKAAGEERKNKLQEEKETLTKHRNAFKKIMEEMNTEYEGLKLQLQENETHAQLTNLERKWQHLEQNNFVMKEFIATKTQESDYRPVVKNVTKQIAEYNKTLIEALENRK

Target ORF information:

RefSeq Version	XM_008103461.2
Organism	Anolis carolinensis(green anole)
Definition	Anolis carolinensis intraflagellar transport 74 (ift74), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008103461.2

ORF Insert Sequence:

ATGGCAAGCA ATCACAAACC TCCTACTGCA CGCCCGTCTT CAAGAGCTGC AATGGGGCTT 
GGAACGCGAC CAACTTCATC TTCATTACGA CCTCCATCTG CAGCTTTACT TGGTGGAGGA 
ATCCCTCCAG GCACCGCAAG GCCTGGTACA CGGGGTGGAT CCATAGGAAC TGGGGTGGTT 
TTGTCTTCTC AAATTAAAGT TGCAGATCGT CCGGTGACAC AGCAAGGACT CAGTGGAATG 
AAAACTGGAA TCAAAGGGCC TCAGAGACAA GTTATGGATA AATCCTATTA TCTGGGCGTG 
CTTAGAAGCA AAATAAGTGA GCTTACTACA GAAATTAACA AGCTTCAGAA AGAAAGGGAC 
ATGTATAATC AGGAGAATTC TGTCTATCTC TCCTATGAAA AAAGAGCAGA ATCTTTAGCT 
GGTGAGATCA AAGACTTTCA GGGACAACTT GCAGACTACA ATATGCTGGT GGATAAACTT 
AACACCAATA CCGATATGGA AGAAGTAATG AGTGACTATA ATTTGCTGAA AGCACAAAAT 
GATCGTGAAG CCCAAGGAAT CGACATTATA TTTACAGAAA GACAAAACAA AGAAAAGCTC 
ATTCAGGCTG TGGAAGATGA CATCCAGCAG GAAAAGCAGG CAGCAGAAAA CATCATCAAA 
AATATGTCTG AAGAAGATCA AGTGAAATAC ATGGAGACAA AAATGGCAAA TGAAAAACTT 
TTACAGGAAT TATCTGCTTT ACAGCAAGAA CTGGATGCTT TGAATATGAA AGAACAAGCG 
CTAGAAGGAG AAATAGCACA TTCCCAGGTT AAACAAGAGG CTCTACAGCT GTATGAAAAG 
CTTCATGGTC TTGAAGAACA TCGAAATCAA ATGATTTTGG AAGATAAAAA TATGGGATCT 
CCCCAAGAAG AGAGAGAGAG ATTATTAAAA CAGGTGAAGG AAGACAATCA AGAAATAGCA 
AGCATGGAAA GACAGTTGAC CGAGATAAAA GAAAAGATTG ATCAAATCAA TGAAGGTATT 
CGTCAGGTTG ACATGGATCT GGAAGAGCAA CAAGGAGAGA AAAATGTGAA ATACAAAGAG 
TTAAAGAAGA GAGAGGAAAG CATGGATAAC TTTCTTGAAT CTTTTGAAGA GACAAAAAAC 
CAGGAACTGG AACGGAAAAC ACAGATAGAG GCCAATATTG TGGCAATTTT AGAGCACTCA 
AGCAGGAATG TAAATCGTAT GAAGCAGATA TCTTCTGTTA CAAATCAAGA GCTGAAGATC 
ATGCAGGAAG ACCTCACATT CAAATCAAGT GAAATGCAAA AATCACAGAG TACAGCAAAA 
AACCTAGGTT CAGACTGTCA ACGTCTGCAG ATGGATCTCC AGAAGATGGG TCTATTGGAA 
GGCAAGATGA AGGACGAACT CGCTTCTCTC AAAGAAAAAA CTGAGCAAAT GACCAAGGAC 
CTAGAACTGT ACAACAACCT GCCAGCTCTG AAAGCAGCAG GAGAAGAGAG AAAGAACAAA 
CTGCAAGAGG AGAAAGAAAC CCTGACAAAA CACAGGAATG CTTTTAAGAA AATTATGGAG 
GAGATGAATA CGGAGTATGA AGGCCTGAAG CTGCAGCTAC AGGAGAATGA GACGCATGCG 
CAGCTTACAA ATCTGGAAAG GAAGTGGCAG CATCTTGAAC AAAATAATTT TGTCATGAAA 
GAATTTATAG CAACAAAAAC CCAAGAAAGT GATTACCGTC CAGTTGTGAA GAATGTCACA 
AAACAGATTG CAGAATATAA CAAAACCCTC ATAGAAGCTT TAGAGAACAG GAAATGA