crim1 cDNA ORF clone,

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gRNAs

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OWo07172	XM_031847133.1 Latest version!	Anarrhichthys ocellatus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_031847133.1 ) cDNA ORF clone, -> XP_031702993.1 Anarrhichthys ocellatus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

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CloneID	OWo07172
Clone ID Related Accession (Same CDS sequence)	XM_031847133.1
Accession Version	XM_031847133.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Anarrhichthys ocellatus(wolf-eel)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022280037.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Anarrhichthys ocellatus Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCA AGTGCTCCGT TGTGTTATTG AATGTCCTTT TTCTGGTGCG GGCGCTCTCG 
GCTCACGAGT GCACGCCGTG TAATCTCCGC ACTTGTCCCG TCAAAGCGCT ACACAGCTGC 
GAGGGTGGCC GGACCCCGGC CAGGGATCCG TGCGGATGCT GTGACCAGTG TGCCCGGCTG 
GAGTGGGAAA CCTGCGGCGG ACAGGACTGG GCGCACGGCT ACTGCGCCTT GGGGCTGACC 
TGCGCCTCTG TTAACAAAAC AGGAGCAGCC GCAATCCCTG AAACTGGAGT ATGTAAAGCG 
CTCCCTAAGC ATCCAGAGGC AGGCCTGGAG GACGAGCGCT GTCCCCTGAT GACAGGCTGC 
GAAAGAGCCG GAGGTCAGTG TGTGTGCGAT GCCCGTCACT CTTGCTTGGG CTCTTTCCAC 
TACCCGGACC AGGAAACGTG CATGAAGGCC AGCAAGTCAG AGGGTCGAAG GCACGAGCAC 
CGGGAAAGAC ACAGAGAGAA GTATGTGGGA CCATCCAACC CGGCCTGCAT GTTCTCTGGG 
TGTAACCTGA CCACCGAGGG CTGTGTGTGT GAGTCCCAGA GCTGCCATCA CCACTTCGCC 
TACATCAACC GCAGCCAGTG CCAGGAAGCT GCAGAGGAGC TGAGGTGTGC CGGGGTGATG 
TGTCCTGCTC TGTCAGTGCC CTCCTGCCCT AAGGGTTCAG TCCTGACCAA GAGTTACACG 
CCCCCTGCTG GTTGCTGCCC CTCCATACTG CCCCAGTGCA CTTGCGACCT CCGTGCCTGC 
CACAAGCTCC AGTGCCCGAG CGGACAGCGC GCCGTGCCGC TCCGCCAGGC CAGCGGCCAG 
CCAGGAGACT GCTGTGATGT CTTCGAGTGC CAGAAAGTCT CTCCCAAGTG TGTCCACAAT 
GGGAAGGAGT TTTCTGAGGG ACAGGTGTAT CGCATGGACC CCTGCTGGCT GTGTCAGTGC 
CGGGGAGGAA TCTCCTTCTG CTCCAAGGCA GAGTGCGCTG AGCTGAACTG TGAAAACTTC 
TACATTCCTG AGGGCGAGTG TTGCCCTGTC TGCATAGATG TGGAGTTACT GTCGATGGAC 
AGCACAAAGG CCAGTTGCTG GGTGAATAAC AAGCTGCGGC CCCACGAGGA GCAGTGGAAA 
GAGGACGACT GCACCTTCTG CCAGTGTGTG GATGGAGAAC CACACTGCAC AGCCATGGCC 
TGCAAACAGA GCTGCCAATA CCCTGTGAAG ATCCCCGGAG AGTGCTGTCC CTTCTGTGAA 
GAGCCTTCTT ATGAAACAGT TAGCCCCCTG GTATGCCCTC CCCTGGAAAA CTGCTCCCTG 
TCAGGGAATG ACTGCCCCTA CGGCTTTCAA CCAGACATAA ATGGATGTCT GCTCTGCCAG 
TGCCTCAACA ATGACTCCTG CCCTGACCTG GGAAAATACT GTTCCCTCCA ATGCCCAGTG 
GGATACAAGA CAGATGATTT CGGCTGTGAG GTGTGTGAAT GCAGCACTCC CGTGTCCAAG 
TGCCGCCCCT TGACCTGCAC TAAGACCTGC CCCTATGGAT ATGTGAGGAA CAAGCATGGC 
TGTGAGATGT GTCGCTGTAT CAAGTGTCCT CCCTTCACCT GTGACAAGCA CTGCAACGAC 
GGCTATAGAC AGAACAGGAA GGGCTGCTCC ATCTGCATGT GTAAAGAAAG TGGCCGCGTC 
CCAAGCACCA CTGCCCCTGC CCCGATTCCC CGCTACTGCC TCACCTCCAA CGGGCACCGA 
TACGAGGAAG GCGACGGCTG GCACGACGGT TGTCGTGACT GCTACTGCCA CTCCGGACGG 
GAGATGTGCG TGCTCATATC CTGCCCCGTG CCCAGCTGTT CTCATCCAGT TGTGAGGCCT 
GACCAGTGTT GTCCTACCTG TGAGGATGAG TCAGGCAGCG GTCAGCTAGA GGGGATGGAC 
ATGGTGGTGT GCAGAGCACC TGGGGGTGAA TTCTATGTGG AGGGAGAAAC CTGGAACCTG 
GACGAGTGCA CACGGTGCAC CTGCAGGAAG GGACGTGTCC TGTGCGACAC CGAAGTGTGT 
CCCCCGGCTC TCTGCCAGAC ACCGATCAGG AACAAGGACA CCTGTTGTCA CGTATGCCCA 
GAGGAGACGC TGAGCCCCTT GCTGCCAGTG AACAACAGCC AGCAGGAGTA CTGCATAACC 
AGCGATGGAG ACGTGCTGCT GTCTGGGGAC TCCTGGAAGG CCAACGCCTG CACCAGCTGC 
ACCTGCAACA ACGGCACCAT TCAGTGTTTC TCTCAGCGCT GTCTGGCTGC CAACTGCAGG 
GTGCCCGTCT TACGCAAGGG CCAGTGCTGC CCACACTGTC TCGAAATTAC AACGCCGGTG 
ACGGTGTCTA CCAACGCCCC CAAGACAACA GCTGCCATTA CAGTGAAGAG TGCGGTGTGG 
ATCACCACTG TCACTGTACC GCCCATCACT GCTGATACAG ATGAGCGAGG TTTGGGTGAG 
AATTACCCCA GCCAAACGGA GATGGCCGTC ATCTACCAAT CAGCCGCCTG GATCCTGGCA 
GGTCTCCTCC TGGCCATCAT CATCTTCATC GCTGTGGCGT TACTCATCAA CAAGAAAAAG 
AAGTGGGTGC AGATGTCCTG CTACAGTGCA CCAAAGAAGA CGGTGATCCT AAAGAAGCAC 
GTCAACAAGA ACTCAGTGGT CTACATGGAG CCCTCCAAGG AGAACAAATT TCAGAATGTG 
AAGAACGAGT GCGGCATCAT CCATTTCTCT CCAGGAATGA GCTCCTCGTG TGCGGACAAG 
ATGACCATCC CCAGGGCCAA GCTGAGCATG GGCAACAACT GGACGGCGCG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031702993.1
CDS	446..3259
Translation	MFSKCSVVLLNVLFLVRALSAHECTPCNLRTCPVKALHSCEGGRTPARDPCGCCDQCARLEWETCGGQDWAHGYCALGLTCASVNKTGAAAIPETGVCKALPKHPEAGLEDERCPLMTGCERAGGQCVCDARHSCLGSFHYPDQETCMKASKSEGRRHEHRERHREKYVGPSNPACMFSGCNLTTEGCVCESQSCHHHFAYINRSQCQEAAEELRCAGVMCPALSVPSCPKGSVLTKSYTPPAGCCPSILPQCTCDLRACHKLQCPSGQRAVPLRQASGQPGDCCDVFECQKVSPKCVHNGKEFSEGQVYRMDPCWLCQCRGGISFCSKAECAELNCENFYIPEGECCPVCIDVELLSMDSTKASCWVNNKLRPHEEQWKEDDCTFCQCVDGEPHCTAMACKQSCQYPVKIPGECCPFCEEPSYETVSPLVCPPLENCSLSGNDCPYGFQPDINGCLLCQCLNNDSCPDLGKYCSLQCPVGYKTDDFGCEVCECSTPVSKCRPLTCTKTCPYGYVRNKHGCEMCRCIKCPPFTCDKHCNDGYRQNRKGCSICMCKESGRVPSTTAPAPIPRYCLTSNGHRYEEGDGWHDGCRDCYCHSGREMCVLISCPVPSCSHPVVRPDQCCPTCEDESGSGQLEGMDMVVCRAPGGEFYVEGETWNLDECTRCTCRKGRVLCDTEVCPPALCQTPIRNKDTCCHVCPEETLSPLLPVNNSQQEYCITSDGDVLLSGDSWKANACTSCTCNNGTIQCFSQRCLAANCRVPVLRKGQCCPHCLEITTPVTVSTNAPKTTAAITVKSAVWITTVTVPPITADTDERGLGENYPSQTEMAVIYQSAAWILAGLLLAIIIFIAVALLINKKKKWVQMSCYSAPKKTVILKKHVNKNSVVYMEPSKENKFQNVKNECGIIHFSPGMSSSCADKMTIPRAKLSMGNNWTAR

Target ORF information:

RefSeq Version	XM_031847133.1
Organism	Anarrhichthys ocellatus(wolf-eel)
Definition	Anarrhichthys ocellatus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031847133.1

ORF Insert Sequence:

ATGTTCAGCA AGTGCTCCGT TGTGTTATTG AATGTCCTTT TTCTGGTGCG GGCGCTCTCG 
GCTCACGAGT GCACGCCGTG TAATCTCCGC ACTTGTCCCG TCAAAGCGCT ACACAGCTGC 
GAGGGTGGCC GGACCCCGGC CAGGGATCCG TGCGGATGCT GTGACCAGTG TGCCCGGCTG 
GAGTGGGAAA CCTGCGGCGG ACAGGACTGG GCGCACGGCT ACTGCGCCTT GGGGCTGACC 
TGCGCCTCTG TTAACAAAAC AGGAGCAGCC GCAATCCCTG AAACTGGAGT ATGTAAAGCG 
CTCCCTAAGC ATCCAGAGGC AGGCCTGGAG GACGAGCGCT GTCCCCTGAT GACAGGCTGC 
GAAAGAGCCG GAGGTCAGTG TGTGTGCGAT GCCCGTCACT CTTGCTTGGG CTCTTTCCAC 
TACCCGGACC AGGAAACGTG CATGAAGGCC AGCAAGTCAG AGGGTCGAAG GCACGAGCAC 
CGGGAAAGAC ACAGAGAGAA GTATGTGGGA CCATCCAACC CGGCCTGCAT GTTCTCTGGG 
TGTAACCTGA CCACCGAGGG CTGTGTGTGT GAGTCCCAGA GCTGCCATCA CCACTTCGCC 
TACATCAACC GCAGCCAGTG CCAGGAAGCT GCAGAGGAGC TGAGGTGTGC CGGGGTGATG 
TGTCCTGCTC TGTCAGTGCC CTCCTGCCCT AAGGGTTCAG TCCTGACCAA GAGTTACACG 
CCCCCTGCTG GTTGCTGCCC CTCCATACTG CCCCAGTGCA CTTGCGACCT CCGTGCCTGC 
CACAAGCTCC AGTGCCCGAG CGGACAGCGC GCCGTGCCGC TCCGCCAGGC CAGCGGCCAG 
CCAGGAGACT GCTGTGATGT CTTCGAGTGC CAGAAAGTCT CTCCCAAGTG TGTCCACAAT 
GGGAAGGAGT TTTCTGAGGG ACAGGTGTAT CGCATGGACC CCTGCTGGCT GTGTCAGTGC 
CGGGGAGGAA TCTCCTTCTG CTCCAAGGCA GAGTGCGCTG AGCTGAACTG TGAAAACTTC 
TACATTCCTG AGGGCGAGTG TTGCCCTGTC TGCATAGATG TGGAGTTACT GTCGATGGAC 
AGCACAAAGG CCAGTTGCTG GGTGAATAAC AAGCTGCGGC CCCACGAGGA GCAGTGGAAA 
GAGGACGACT GCACCTTCTG CCAGTGTGTG GATGGAGAAC CACACTGCAC AGCCATGGCC 
TGCAAACAGA GCTGCCAATA CCCTGTGAAG ATCCCCGGAG AGTGCTGTCC CTTCTGTGAA 
GAGCCTTCTT ATGAAACAGT TAGCCCCCTG GTATGCCCTC CCCTGGAAAA CTGCTCCCTG 
TCAGGGAATG ACTGCCCCTA CGGCTTTCAA CCAGACATAA ATGGATGTCT GCTCTGCCAG 
TGCCTCAACA ATGACTCCTG CCCTGACCTG GGAAAATACT GTTCCCTCCA ATGCCCAGTG 
GGATACAAGA CAGATGATTT CGGCTGTGAG GTGTGTGAAT GCAGCACTCC CGTGTCCAAG 
TGCCGCCCCT TGACCTGCAC TAAGACCTGC CCCTATGGAT ATGTGAGGAA CAAGCATGGC 
TGTGAGATGT GTCGCTGTAT CAAGTGTCCT CCCTTCACCT GTGACAAGCA CTGCAACGAC 
GGCTATAGAC AGAACAGGAA GGGCTGCTCC ATCTGCATGT GTAAAGAAAG TGGCCGCGTC 
CCAAGCACCA CTGCCCCTGC CCCGATTCCC CGCTACTGCC TCACCTCCAA CGGGCACCGA 
TACGAGGAAG GCGACGGCTG GCACGACGGT TGTCGTGACT GCTACTGCCA CTCCGGACGG 
GAGATGTGCG TGCTCATATC CTGCCCCGTG CCCAGCTGTT CTCATCCAGT TGTGAGGCCT 
GACCAGTGTT GTCCTACCTG TGAGGATGAG TCAGGCAGCG GTCAGCTAGA GGGGATGGAC 
ATGGTGGTGT GCAGAGCACC TGGGGGTGAA TTCTATGTGG AGGGAGAAAC CTGGAACCTG 
GACGAGTGCA CACGGTGCAC CTGCAGGAAG GGACGTGTCC TGTGCGACAC CGAAGTGTGT 
CCCCCGGCTC TCTGCCAGAC ACCGATCAGG AACAAGGACA CCTGTTGTCA CGTATGCCCA 
GAGGAGACGC TGAGCCCCTT GCTGCCAGTG AACAACAGCC AGCAGGAGTA CTGCATAACC 
AGCGATGGAG ACGTGCTGCT GTCTGGGGAC TCCTGGAAGG CCAACGCCTG CACCAGCTGC 
ACCTGCAACA ACGGCACCAT TCAGTGTTTC TCTCAGCGCT GTCTGGCTGC CAACTGCAGG 
GTGCCCGTCT TACGCAAGGG CCAGTGCTGC CCACACTGTC TCGAAATTAC AACGCCGGTG 
ACGGTGTCTA CCAACGCCCC CAAGACAACA GCTGCCATTA CAGTGAAGAG TGCGGTGTGG 
ATCACCACTG TCACTGTACC GCCCATCACT GCTGATACAG ATGAGCGAGG TTTGGGTGAG 
AATTACCCCA GCCAAACGGA GATGGCCGTC ATCTACCAAT CAGCCGCCTG GATCCTGGCA 
GGTCTCCTCC TGGCCATCAT CATCTTCATC GCTGTGGCGT TACTCATCAA CAAGAAAAAG 
AAGTGGGTGC AGATGTCCTG CTACAGTGCA CCAAAGAAGA CGGTGATCCT AAAGAAGCAC 
GTCAACAAGA ACTCAGTGGT CTACATGGAG CCCTCCAAGG AGAACAAATT TCAGAATGTG 
AAGAACGAGT GCGGCATCAT CCATTTCTCT CCAGGAATGA GCTCCTCGTG TGCGGACAAG 
ATGACCATCC CCAGGGCCAA GCTGAGCATG GGCAACAACT GGACGGCGCG CTAA