LOC109713715 cDNA ORF clone, Ananas comosus(pineapple)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109713715
Entrez Gene ID	109713715
Full Name	pentatricopeptide repeat-containing protein At5g61990, mitochondrial
Gene Type	protein-coding
Organism	Ananas comosus(pineapple)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020237904.1	XP_020093493.1	pentatricopeptide repeat-containing protein At5g61990, mitochondrial
XM_020237895.1	XP_020093484.1	pentatricopeptide repeat-containing protein At5g61990, mitochondrial

Pathways from BioSystems
Gene Ontology

The following LOC109713715 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109713715 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAn58976	XM_020237895.1 Latest version!	Ananas comosus pentatricopeptide repeat-containing protein At5g61990, mitochondrial (LOC109713715), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAn58976	XM_020237904.1 Latest version!	Ananas comosus pentatricopeptide repeat-containing protein At5g61990, mitochondrial (LOC109713715), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109713715 ( XM_020237904.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020093493.1 Ananas comosus pentatricopeptide repeat-containing protein At5g61990, mitochondrial (LOC109713715), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAn58976
Clone ID Related Accession (Same CDS sequence)	XM_020237904.1 , XM_020237895.1
Accession Version	XM_020237904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	pentatricopeptide repeat-containing protein At5g61990, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033621.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGAA TCGCGCGACA CCCCACTCTC CCCCTTCCTC TCCCACCGAA ACCCTGGCTC 
CTTTCACGAA ACCCTAATCC CCGATCCTCC CACCTCTCCT CCTCCTCCTC CTCCTCCTCC 
TCCTCCGCCG CCGCCGAGGA CGATGACGAC GTGGCGCGGC AGATCACCGT CCTCCTCGGT 
CGGAGCGCGG GGTGGAGGGC CGTCTGCGCC GCCTCAGACC TCCCCCGCCG CCTCACCCCG 
GGCGCCGTCT CCGCCGCGCT ACTCCGCGGC GGCGCCCTCG ACCCCAAGCT CCTCCTCGAC 
TTCTTCTACT GGTCCCTCCC CCTCCTCCCC TCCGCTCCCC TCCCCGACTC CTTCGCCCTC 
CTCGCGCTCC GCCTCTCCAA CTGCGGCCTC TTCGGCCCCG CCAATGGCCT CCTCGAGCGC 
ATGATCAAGT CCCACCCGAA CCCCCCCACC GTGCTCAATT CGATCGCCCA TCGCTTCTCC 
CAGCGCGAAG GTAACAATCC TTTGGTGTTC GATGTGCTTA TCGATACTTA TAAGAAGATG 
GGCATGCTGA GGGACGCCGC AGAAGTCGTT TTGCTCATGA AAGGCGCGAG CTTCGCGCCG 
AGCTTGCGGT GTTGCAACGC TCTCTTGAAG GATCTGTTGA GGGCTAATTT GATGGATTTG 
TTCTGGAAGA TTTATGATTT CATGTTGGGA GCGAAATTGG GTCGTGACGT GTACACTTTT 
AATGCTTTGA TCGAAGCTTT TTTTAAGGTC GGGAACACGG ATTCGGCCAA AAGGGTCTTC 
TTTGAGATGG AAAGCAATGG GTGTACTCCG AATGCCGTGA CGTATAACAC TGTGATTGCT 
GGGTTTTGTA GAGTCAGAGC TGTAGAGGAT GCTTTCAAGT TTAAGAAGGA AATGGTGGAG 
AGAGGATTTG TTGTGGATAG CTTTACTTAC GGCTCGCTGA TCAATGGCTT ATGCAAAGTT 
CACAAGTTGG CGGATGCACA GCAACTGCTT GATGAAATGT CTAGCTTAGG CATAAAGCCG 
GGTATAGTCG TTTATAATAC GTTGATTGAT GGGTATATGA GAGAAGGCAA TGTGGATGAA 
GCCTCCAAGA TAATGGATCA GATGATTGCT TCTGGCGTGC ATCGAAACAA ATTCACATTC 
GACAATCTCA TTCGTGGAGC AGGCAAGATG GGTAAGATTG ATAAGGCCTA TGAACTTTTA 
AAAGAGATGG ATAGAATGTG TTACAAGCCG GACACAATGA CTTATAATTT GATAATAGAG 
GGGCATTTTA AAGCTAATAA TACGAAAGGG GCATTTCTGT TGCTCGATGA AATGAGTAGA 
CTAAATGTAT CACCTAATGT ATATACCTAC AGTGCGATGA TCAATGGGCT TTGCCATTGT 
GGGGAATCGA GGCAAGCCGA TGGTCTTTTA AAGGAGATGG TTTTAAAAGG TTTGGAGCCT 
AACGTTGTAA TTTATGCGGC TCTTATTTCG GGTCATTGTA GGGAAGGTAA TTTTTCAGAG 
GCTTGCCAGA TAATAAATAA AATGATTGAG AGAAATATTT CTCCTGATTC ATATTGCTAT 
AATTCTCTTA TTAGCGGACT TTCTAGGGCT GGAAAAATGG AAGAAGCTAA ACATTATTTT 
GCTAAAATGC AAGAGAGAGG GTTGTCACAC ACTTCATTCA CATACGGGGC CCTTATTCAT 
GGTTACAGTA AAGCTGGGGA TATGGAGAAG GCTGACTTTT ATTTTCAGGA GATGCTCAGT 
AAAGGATTAA AACCTAATGA TGTTATTTAT ACGACTCTCC TTGACGGTCA CTGCAAATTG 
AATGACATTG CTAAGGCCAA ATCGACACTT CAGTCCATGT TGGCAAGGGG CATCATGCCA 
GACATCCAAA CTTACGGTGT GTTAATTAAT AGCCTTTTAA AAAGTGGAAA CATTGATCTG 
GCTTTTAAAA CCCTGTCCGA GCTCGAAAAT AAAGGCTTAG TTCCTGATGC ATATATCTAT 
AGCTCGTTGA TCGTTGCGCT TTGCAAGTCG GGTGATGTAG AAAAAGCTTT TGGTCTTCTT 
GATGAAATGG TGAGCAAAGG TGTAGAGCCA AATATTGTTA CTTATAATGC CTTAGTGGAT 
GGTCTTTGCA AATTGGGTGA TGTTAAAAGT GCTAGTAATG TTTTCAATAA CATTTTAGAG 
AAAGGGCCGA TGCCAAATAG TGTAACATAC ACTAGTATGA TTGATGGAAA TTGCAAGAAC 
GGTGATATAT GTGAGGCTTT TAGACTATAC AATGAGATGA TATCAAGAGG GATAGAGGCG 
GATAAGTTTG TTTATACAGC TCTCATTGGC GGCTGCTCCA GTGTAGGGGA CTTTGAGAAG 
GCTTTAGAGT TGATTGACGT GATGTTAGTA AAAGGGTTCG CAACTGTTGC CACATTCAAC 
ACTTTGATTA ATGGCTTCTG CAAGCTTGGA AAGCTACAAG AAGCTAGTAA GTTATTAGAT 
CTAATGGTGG AAAAGCAAAT ACTGCCTAAT TTTGCTACCT ATTCAACTTT ATTTGCGGGA 
TTTGACAGGG CCGAAAAGGT AGACGAAGCA TTTCAGTTAT TTATTCAGAT AGAGGAGAAG 
AACCTTAGAC CGAATGCTGT CATTTATATG TCTGAATTAT TTAAAGATAT GGTAAATAAA 
GGCATATTGT CTGATAAAAT TACTGATAGA ATATTTCAGT TGCATTGCAA AGAAGGAAAT 
GTGGTCGAGG CCTTAAAATT GCGTGAAGCT ATTATTGCAG GAGGCACAAA AATTAGCAGT 
GCAGCATATT TTTTATTGGT GGATGGCCTT TGTAGGATGG GGAACTTACC TGAAGCAATT 
GAGTTATTTA ATGAAATGAA AGAACAAGAA ATCAAGCCTG ACGATGCACA ATGCCTGATG 
CTACTCCGTC GTCTTTACAC AAATGGGTTT ATTGATGAAT CCGATAAGAT TTTGGGAATT 
TTATTATGCA ATAATTGGTT GCAAGAAGGC ACTAAATTGA GCGATATGGG AGAGGAGGAT 
CAGGACATTG TGAATGGCGA GTCGGTGATT AAGGTGGAAG GTGTGCTTAA GGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020093493.1
CDS	166..3222
Translation	MLRIARHPTLPLPLPPKPWLLSRNPNPRSSHLSSSSSSSSSSAAAEDDDDVARQITVLLGRSAGWRAVCAASDLPRRLTPGAVSAALLRGGALDPKLLLDFFYWSLPLLPSAPLPDSFALLALRLSNCGLFGPANGLLERMIKSHPNPPTVLNSIAHRFSQREGNNPLVFDVLIDTYKKMGMLRDAAEVVLLMKGASFAPSLRCCNALLKDLLRANLMDLFWKIYDFMLGAKLGRDVYTFNALIEAFFKVGNTDSAKRVFFEMESNGCTPNAVTYNTVIAGFCRVRAVEDAFKFKKEMVERGFVVDSFTYGSLINGLCKVHKLADAQQLLDEMSSLGIKPGIVVYNTLIDGYMREGNVDEASKIMDQMIASGVHRNKFTFDNLIRGAGKMGKIDKAYELLKEMDRMCYKPDTMTYNLIIEGHFKANNTKGAFLLLDEMSRLNVSPNVYTYSAMINGLCHCGESRQADGLLKEMVLKGLEPNVVIYAALISGHCREGNFSEACQIINKMIERNISPDSYCYNSLISGLSRAGKMEEAKHYFAKMQERGLSHTSFTYGALIHGYSKAGDMEKADFYFQEMLSKGLKPNDVIYTTLLDGHCKLNDIAKAKSTLQSMLARGIMPDIQTYGVLINSLLKSGNIDLAFKTLSELENKGLVPDAYIYSSLIVALCKSGDVEKAFGLLDEMVSKGVEPNIVTYNALVDGLCKLGDVKSASNVFNNILEKGPMPNSVTYTSMIDGNCKNGDICEAFRLYNEMISRGIEADKFVYTALIGGCSSVGDFEKALELIDVMLVKGFATVATFNTLINGFCKLGKLQEASKLLDLMVEKQILPNFATYSTLFAGFDRAEKVDEAFQLFIQIEEKNLRPNAVIYMSELFKDMVNKGILSDKITDRIFQLHCKEGNVVEALKLREAIIAGGTKISSAAYFLLVDGLCRMGNLPEAIELFNEMKEQEIKPDDAQCLMLLRRLYTNGFIDESDKILGILLCNNWLQEGTKLSDMGEEDQDIVNGESVIKVEGVLKA

Target ORF information:

RefSeq Version	XM_020237904.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus pentatricopeptide repeat-containing protein At5g61990, mitochondrial (LOC109713715), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020237904.1

ORF Insert Sequence:

ATGCTCCGAA TCGCGCGACA CCCCACTCTC CCCCTTCCTC TCCCACCGAA ACCCTGGCTC 
CTTTCACGAA ACCCTAATCC CCGATCCTCC CACCTCTCCT CCTCCTCCTC CTCCTCCTCC 
TCCTCCGCCG CCGCCGAGGA CGATGACGAC GTGGCGCGGC AGATCACCGT CCTCCTCGGT 
CGGAGCGCGG GGTGGAGGGC CGTCTGCGCC GCCTCAGACC TCCCCCGCCG CCTCACCCCG 
GGCGCCGTCT CCGCCGCGCT ACTCCGCGGC GGCGCCCTCG ACCCCAAGCT CCTCCTCGAC 
TTCTTCTACT GGTCCCTCCC CCTCCTCCCC TCCGCTCCCC TCCCCGACTC CTTCGCCCTC 
CTCGCGCTCC GCCTCTCCAA CTGCGGCCTC TTCGGCCCCG CCAATGGCCT CCTCGAGCGC 
ATGATCAAGT CCCACCCGAA CCCCCCCACC GTGCTCAATT CGATCGCCCA TCGCTTCTCC 
CAGCGCGAAG GTAACAATCC TTTGGTGTTC GATGTGCTTA TCGATACTTA TAAGAAGATG 
GGCATGCTGA GGGACGCCGC AGAAGTCGTT TTGCTCATGA AAGGCGCGAG CTTCGCGCCG 
AGCTTGCGGT GTTGCAACGC TCTCTTGAAG GATCTGTTGA GGGCTAATTT GATGGATTTG 
TTCTGGAAGA TTTATGATTT CATGTTGGGA GCGAAATTGG GTCGTGACGT GTACACTTTT 
AATGCTTTGA TCGAAGCTTT TTTTAAGGTC GGGAACACGG ATTCGGCCAA AAGGGTCTTC 
TTTGAGATGG AAAGCAATGG GTGTACTCCG AATGCCGTGA CGTATAACAC TGTGATTGCT 
GGGTTTTGTA GAGTCAGAGC TGTAGAGGAT GCTTTCAAGT TTAAGAAGGA AATGGTGGAG 
AGAGGATTTG TTGTGGATAG CTTTACTTAC GGCTCGCTGA TCAATGGCTT ATGCAAAGTT 
CACAAGTTGG CGGATGCACA GCAACTGCTT GATGAAATGT CTAGCTTAGG CATAAAGCCG 
GGTATAGTCG TTTATAATAC GTTGATTGAT GGGTATATGA GAGAAGGCAA TGTGGATGAA 
GCCTCCAAGA TAATGGATCA GATGATTGCT TCTGGCGTGC ATCGAAACAA ATTCACATTC 
GACAATCTCA TTCGTGGAGC AGGCAAGATG GGTAAGATTG ATAAGGCCTA TGAACTTTTA 
AAAGAGATGG ATAGAATGTG TTACAAGCCG GACACAATGA CTTATAATTT GATAATAGAG 
GGGCATTTTA AAGCTAATAA TACGAAAGGG GCATTTCTGT TGCTCGATGA AATGAGTAGA 
CTAAATGTAT CACCTAATGT ATATACCTAC AGTGCGATGA TCAATGGGCT TTGCCATTGT 
GGGGAATCGA GGCAAGCCGA TGGTCTTTTA AAGGAGATGG TTTTAAAAGG TTTGGAGCCT 
AACGTTGTAA TTTATGCGGC TCTTATTTCG GGTCATTGTA GGGAAGGTAA TTTTTCAGAG 
GCTTGCCAGA TAATAAATAA AATGATTGAG AGAAATATTT CTCCTGATTC ATATTGCTAT 
AATTCTCTTA TTAGCGGACT TTCTAGGGCT GGAAAAATGG AAGAAGCTAA ACATTATTTT 
GCTAAAATGC AAGAGAGAGG GTTGTCACAC ACTTCATTCA CATACGGGGC CCTTATTCAT 
GGTTACAGTA AAGCTGGGGA TATGGAGAAG GCTGACTTTT ATTTTCAGGA GATGCTCAGT 
AAAGGATTAA AACCTAATGA TGTTATTTAT ACGACTCTCC TTGACGGTCA CTGCAAATTG 
AATGACATTG CTAAGGCCAA ATCGACACTT CAGTCCATGT TGGCAAGGGG CATCATGCCA 
GACATCCAAA CTTACGGTGT GTTAATTAAT AGCCTTTTAA AAAGTGGAAA CATTGATCTG 
GCTTTTAAAA CCCTGTCCGA GCTCGAAAAT AAAGGCTTAG TTCCTGATGC ATATATCTAT 
AGCTCGTTGA TCGTTGCGCT TTGCAAGTCG GGTGATGTAG AAAAAGCTTT TGGTCTTCTT 
GATGAAATGG TGAGCAAAGG TGTAGAGCCA AATATTGTTA CTTATAATGC CTTAGTGGAT 
GGTCTTTGCA AATTGGGTGA TGTTAAAAGT GCTAGTAATG TTTTCAATAA CATTTTAGAG 
AAAGGGCCGA TGCCAAATAG TGTAACATAC ACTAGTATGA TTGATGGAAA TTGCAAGAAC 
GGTGATATAT GTGAGGCTTT TAGACTATAC AATGAGATGA TATCAAGAGG GATAGAGGCG 
GATAAGTTTG TTTATACAGC TCTCATTGGC GGCTGCTCCA GTGTAGGGGA CTTTGAGAAG 
GCTTTAGAGT TGATTGACGT GATGTTAGTA AAAGGGTTCG CAACTGTTGC CACATTCAAC 
ACTTTGATTA ATGGCTTCTG CAAGCTTGGA AAGCTACAAG AAGCTAGTAA GTTATTAGAT 
CTAATGGTGG AAAAGCAAAT ACTGCCTAAT TTTGCTACCT ATTCAACTTT ATTTGCGGGA 
TTTGACAGGG CCGAAAAGGT AGACGAAGCA TTTCAGTTAT TTATTCAGAT AGAGGAGAAG 
AACCTTAGAC CGAATGCTGT CATTTATATG TCTGAATTAT TTAAAGATAT GGTAAATAAA 
GGCATATTGT CTGATAAAAT TACTGATAGA ATATTTCAGT TGCATTGCAA AGAAGGAAAT 
GTGGTCGAGG CCTTAAAATT GCGTGAAGCT ATTATTGCAG GAGGCACAAA AATTAGCAGT 
GCAGCATATT TTTTATTGGT GGATGGCCTT TGTAGGATGG GGAACTTACC TGAAGCAATT 
GAGTTATTTA ATGAAATGAA AGAACAAGAA ATCAAGCCTG ACGATGCACA ATGCCTGATG 
CTACTCCGTC GTCTTTACAC AAATGGGTTT ATTGATGAAT CCGATAAGAT TTTGGGAATT 
TTATTATGCA ATAATTGGTT GCAAGAAGGC ACTAAATTGA GCGATATGGG AGAGGAGGAT 
CAGGACATTG TGAATGGCGA GTCGGTGATT AAGGTGGAAG GTGTGCTTAA GGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109713715 ( XM_020237895.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020093484.1 Ananas comosus pentatricopeptide repeat-containing protein At5g61990, mitochondrial (LOC109713715), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAn58976
Clone ID Related Accession (Same CDS sequence)	XM_020237904.1 , XM_020237895.1
Accession Version	XM_020237895.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	pentatricopeptide repeat-containing protein At5g61990, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033621.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGAA TCGCGCGACA CCCCACTCTC CCCCTTCCTC TCCCACCGAA ACCCTGGCTC 
CTTTCACGAA ACCCTAATCC CCGATCCTCC CACCTCTCCT CCTCCTCCTC CTCCTCCTCC 
TCCTCCGCCG CCGCCGAGGA CGATGACGAC GTGGCGCGGC AGATCACCGT CCTCCTCGGT 
CGGAGCGCGG GGTGGAGGGC CGTCTGCGCC GCCTCAGACC TCCCCCGCCG CCTCACCCCG 
GGCGCCGTCT CCGCCGCGCT ACTCCGCGGC GGCGCCCTCG ACCCCAAGCT CCTCCTCGAC 
TTCTTCTACT GGTCCCTCCC CCTCCTCCCC TCCGCTCCCC TCCCCGACTC CTTCGCCCTC 
CTCGCGCTCC GCCTCTCCAA CTGCGGCCTC TTCGGCCCCG CCAATGGCCT CCTCGAGCGC 
ATGATCAAGT CCCACCCGAA CCCCCCCACC GTGCTCAATT CGATCGCCCA TCGCTTCTCC 
CAGCGCGAAG GTAACAATCC TTTGGTGTTC GATGTGCTTA TCGATACTTA TAAGAAGATG 
GGCATGCTGA GGGACGCCGC AGAAGTCGTT TTGCTCATGA AAGGCGCGAG CTTCGCGCCG 
AGCTTGCGGT GTTGCAACGC TCTCTTGAAG GATCTGTTGA GGGCTAATTT GATGGATTTG 
TTCTGGAAGA TTTATGATTT CATGTTGGGA GCGAAATTGG GTCGTGACGT GTACACTTTT 
AATGCTTTGA TCGAAGCTTT TTTTAAGGTC GGGAACACGG ATTCGGCCAA AAGGGTCTTC 
TTTGAGATGG AAAGCAATGG GTGTACTCCG AATGCCGTGA CGTATAACAC TGTGATTGCT 
GGGTTTTGTA GAGTCAGAGC TGTAGAGGAT GCTTTCAAGT TTAAGAAGGA AATGGTGGAG 
AGAGGATTTG TTGTGGATAG CTTTACTTAC GGCTCGCTGA TCAATGGCTT ATGCAAAGTT 
CACAAGTTGG CGGATGCACA GCAACTGCTT GATGAAATGT CTAGCTTAGG CATAAAGCCG 
GGTATAGTCG TTTATAATAC GTTGATTGAT GGGTATATGA GAGAAGGCAA TGTGGATGAA 
GCCTCCAAGA TAATGGATCA GATGATTGCT TCTGGCGTGC ATCGAAACAA ATTCACATTC 
GACAATCTCA TTCGTGGAGC AGGCAAGATG GGTAAGATTG ATAAGGCCTA TGAACTTTTA 
AAAGAGATGG ATAGAATGTG TTACAAGCCG GACACAATGA CTTATAATTT GATAATAGAG 
GGGCATTTTA AAGCTAATAA TACGAAAGGG GCATTTCTGT TGCTCGATGA AATGAGTAGA 
CTAAATGTAT CACCTAATGT ATATACCTAC AGTGCGATGA TCAATGGGCT TTGCCATTGT 
GGGGAATCGA GGCAAGCCGA TGGTCTTTTA AAGGAGATGG TTTTAAAAGG TTTGGAGCCT 
AACGTTGTAA TTTATGCGGC TCTTATTTCG GGTCATTGTA GGGAAGGTAA TTTTTCAGAG 
GCTTGCCAGA TAATAAATAA AATGATTGAG AGAAATATTT CTCCTGATTC ATATTGCTAT 
AATTCTCTTA TTAGCGGACT TTCTAGGGCT GGAAAAATGG AAGAAGCTAA ACATTATTTT 
GCTAAAATGC AAGAGAGAGG GTTGTCACAC ACTTCATTCA CATACGGGGC CCTTATTCAT 
GGTTACAGTA AAGCTGGGGA TATGGAGAAG GCTGACTTTT ATTTTCAGGA GATGCTCAGT 
AAAGGATTAA AACCTAATGA TGTTATTTAT ACGACTCTCC TTGACGGTCA CTGCAAATTG 
AATGACATTG CTAAGGCCAA ATCGACACTT CAGTCCATGT TGGCAAGGGG CATCATGCCA 
GACATCCAAA CTTACGGTGT GTTAATTAAT AGCCTTTTAA AAAGTGGAAA CATTGATCTG 
GCTTTTAAAA CCCTGTCCGA GCTCGAAAAT AAAGGCTTAG TTCCTGATGC ATATATCTAT 
AGCTCGTTGA TCGTTGCGCT TTGCAAGTCG GGTGATGTAG AAAAAGCTTT TGGTCTTCTT 
GATGAAATGG TGAGCAAAGG TGTAGAGCCA AATATTGTTA CTTATAATGC CTTAGTGGAT 
GGTCTTTGCA AATTGGGTGA TGTTAAAAGT GCTAGTAATG TTTTCAATAA CATTTTAGAG 
AAAGGGCCGA TGCCAAATAG TGTAACATAC ACTAGTATGA TTGATGGAAA TTGCAAGAAC 
GGTGATATAT GTGAGGCTTT TAGACTATAC AATGAGATGA TATCAAGAGG GATAGAGGCG 
GATAAGTTTG TTTATACAGC TCTCATTGGC GGCTGCTCCA GTGTAGGGGA CTTTGAGAAG 
GCTTTAGAGT TGATTGACGT GATGTTAGTA AAAGGGTTCG CAACTGTTGC CACATTCAAC 
ACTTTGATTA ATGGCTTCTG CAAGCTTGGA AAGCTACAAG AAGCTAGTAA GTTATTAGAT 
CTAATGGTGG AAAAGCAAAT ACTGCCTAAT TTTGCTACCT ATTCAACTTT ATTTGCGGGA 
TTTGACAGGG CCGAAAAGGT AGACGAAGCA TTTCAGTTAT TTATTCAGAT AGAGGAGAAG 
AACCTTAGAC CGAATGCTGT CATTTATATG TCTGAATTAT TTAAAGATAT GGTAAATAAA 
GGCATATTGT CTGATAAAAT TACTGATAGA ATATTTCAGT TGCATTGCAA AGAAGGAAAT 
GTGGTCGAGG CCTTAAAATT GCGTGAAGCT ATTATTGCAG GAGGCACAAA AATTAGCAGT 
GCAGCATATT TTTTATTGGT GGATGGCCTT TGTAGGATGG GGAACTTACC TGAAGCAATT 
GAGTTATTTA ATGAAATGAA AGAACAAGAA ATCAAGCCTG ACGATGCACA ATGCCTGATG 
CTACTCCGTC GTCTTTACAC AAATGGGTTT ATTGATGAAT CCGATAAGAT TTTGGGAATT 
TTATTATGCA ATAATTGGTT GCAAGAAGGC ACTAAATTGA GCGATATGGG AGAGGAGGAT 
CAGGACATTG TGAATGGCGA GTCGGTGATT AAGGTGGAAG GTGTGCTTAA GGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020093484.1
CDS	166..3222
Translation	MLRIARHPTLPLPLPPKPWLLSRNPNPRSSHLSSSSSSSSSSAAAEDDDDVARQITVLLGRSAGWRAVCAASDLPRRLTPGAVSAALLRGGALDPKLLLDFFYWSLPLLPSAPLPDSFALLALRLSNCGLFGPANGLLERMIKSHPNPPTVLNSIAHRFSQREGNNPLVFDVLIDTYKKMGMLRDAAEVVLLMKGASFAPSLRCCNALLKDLLRANLMDLFWKIYDFMLGAKLGRDVYTFNALIEAFFKVGNTDSAKRVFFEMESNGCTPNAVTYNTVIAGFCRVRAVEDAFKFKKEMVERGFVVDSFTYGSLINGLCKVHKLADAQQLLDEMSSLGIKPGIVVYNTLIDGYMREGNVDEASKIMDQMIASGVHRNKFTFDNLIRGAGKMGKIDKAYELLKEMDRMCYKPDTMTYNLIIEGHFKANNTKGAFLLLDEMSRLNVSPNVYTYSAMINGLCHCGESRQADGLLKEMVLKGLEPNVVIYAALISGHCREGNFSEACQIINKMIERNISPDSYCYNSLISGLSRAGKMEEAKHYFAKMQERGLSHTSFTYGALIHGYSKAGDMEKADFYFQEMLSKGLKPNDVIYTTLLDGHCKLNDIAKAKSTLQSMLARGIMPDIQTYGVLINSLLKSGNIDLAFKTLSELENKGLVPDAYIYSSLIVALCKSGDVEKAFGLLDEMVSKGVEPNIVTYNALVDGLCKLGDVKSASNVFNNILEKGPMPNSVTYTSMIDGNCKNGDICEAFRLYNEMISRGIEADKFVYTALIGGCSSVGDFEKALELIDVMLVKGFATVATFNTLINGFCKLGKLQEASKLLDLMVEKQILPNFATYSTLFAGFDRAEKVDEAFQLFIQIEEKNLRPNAVIYMSELFKDMVNKGILSDKITDRIFQLHCKEGNVVEALKLREAIIAGGTKISSAAYFLLVDGLCRMGNLPEAIELFNEMKEQEIKPDDAQCLMLLRRLYTNGFIDESDKILGILLCNNWLQEGTKLSDMGEEDQDIVNGESVIKVEGVLKA

Target ORF information:

RefSeq Version	XM_020237895.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus pentatricopeptide repeat-containing protein At5g61990, mitochondrial (LOC109713715), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020237895.1

ORF Insert Sequence:

ATGCTCCGAA TCGCGCGACA CCCCACTCTC CCCCTTCCTC TCCCACCGAA ACCCTGGCTC 
CTTTCACGAA ACCCTAATCC CCGATCCTCC CACCTCTCCT CCTCCTCCTC CTCCTCCTCC 
TCCTCCGCCG CCGCCGAGGA CGATGACGAC GTGGCGCGGC AGATCACCGT CCTCCTCGGT 
CGGAGCGCGG GGTGGAGGGC CGTCTGCGCC GCCTCAGACC TCCCCCGCCG CCTCACCCCG 
GGCGCCGTCT CCGCCGCGCT ACTCCGCGGC GGCGCCCTCG ACCCCAAGCT CCTCCTCGAC 
TTCTTCTACT GGTCCCTCCC CCTCCTCCCC TCCGCTCCCC TCCCCGACTC CTTCGCCCTC 
CTCGCGCTCC GCCTCTCCAA CTGCGGCCTC TTCGGCCCCG CCAATGGCCT CCTCGAGCGC 
ATGATCAAGT CCCACCCGAA CCCCCCCACC GTGCTCAATT CGATCGCCCA TCGCTTCTCC 
CAGCGCGAAG GTAACAATCC TTTGGTGTTC GATGTGCTTA TCGATACTTA TAAGAAGATG 
GGCATGCTGA GGGACGCCGC AGAAGTCGTT TTGCTCATGA AAGGCGCGAG CTTCGCGCCG 
AGCTTGCGGT GTTGCAACGC TCTCTTGAAG GATCTGTTGA GGGCTAATTT GATGGATTTG 
TTCTGGAAGA TTTATGATTT CATGTTGGGA GCGAAATTGG GTCGTGACGT GTACACTTTT 
AATGCTTTGA TCGAAGCTTT TTTTAAGGTC GGGAACACGG ATTCGGCCAA AAGGGTCTTC 
TTTGAGATGG AAAGCAATGG GTGTACTCCG AATGCCGTGA CGTATAACAC TGTGATTGCT 
GGGTTTTGTA GAGTCAGAGC TGTAGAGGAT GCTTTCAAGT TTAAGAAGGA AATGGTGGAG 
AGAGGATTTG TTGTGGATAG CTTTACTTAC GGCTCGCTGA TCAATGGCTT ATGCAAAGTT 
CACAAGTTGG CGGATGCACA GCAACTGCTT GATGAAATGT CTAGCTTAGG CATAAAGCCG 
GGTATAGTCG TTTATAATAC GTTGATTGAT GGGTATATGA GAGAAGGCAA TGTGGATGAA 
GCCTCCAAGA TAATGGATCA GATGATTGCT TCTGGCGTGC ATCGAAACAA ATTCACATTC 
GACAATCTCA TTCGTGGAGC AGGCAAGATG GGTAAGATTG ATAAGGCCTA TGAACTTTTA 
AAAGAGATGG ATAGAATGTG TTACAAGCCG GACACAATGA CTTATAATTT GATAATAGAG 
GGGCATTTTA AAGCTAATAA TACGAAAGGG GCATTTCTGT TGCTCGATGA AATGAGTAGA 
CTAAATGTAT CACCTAATGT ATATACCTAC AGTGCGATGA TCAATGGGCT TTGCCATTGT 
GGGGAATCGA GGCAAGCCGA TGGTCTTTTA AAGGAGATGG TTTTAAAAGG TTTGGAGCCT 
AACGTTGTAA TTTATGCGGC TCTTATTTCG GGTCATTGTA GGGAAGGTAA TTTTTCAGAG 
GCTTGCCAGA TAATAAATAA AATGATTGAG AGAAATATTT CTCCTGATTC ATATTGCTAT 
AATTCTCTTA TTAGCGGACT TTCTAGGGCT GGAAAAATGG AAGAAGCTAA ACATTATTTT 
GCTAAAATGC AAGAGAGAGG GTTGTCACAC ACTTCATTCA CATACGGGGC CCTTATTCAT 
GGTTACAGTA AAGCTGGGGA TATGGAGAAG GCTGACTTTT ATTTTCAGGA GATGCTCAGT 
AAAGGATTAA AACCTAATGA TGTTATTTAT ACGACTCTCC TTGACGGTCA CTGCAAATTG 
AATGACATTG CTAAGGCCAA ATCGACACTT CAGTCCATGT TGGCAAGGGG CATCATGCCA 
GACATCCAAA CTTACGGTGT GTTAATTAAT AGCCTTTTAA AAAGTGGAAA CATTGATCTG 
GCTTTTAAAA CCCTGTCCGA GCTCGAAAAT AAAGGCTTAG TTCCTGATGC ATATATCTAT 
AGCTCGTTGA TCGTTGCGCT TTGCAAGTCG GGTGATGTAG AAAAAGCTTT TGGTCTTCTT 
GATGAAATGG TGAGCAAAGG TGTAGAGCCA AATATTGTTA CTTATAATGC CTTAGTGGAT 
GGTCTTTGCA AATTGGGTGA TGTTAAAAGT GCTAGTAATG TTTTCAATAA CATTTTAGAG 
AAAGGGCCGA TGCCAAATAG TGTAACATAC ACTAGTATGA TTGATGGAAA TTGCAAGAAC 
GGTGATATAT GTGAGGCTTT TAGACTATAC AATGAGATGA TATCAAGAGG GATAGAGGCG 
GATAAGTTTG TTTATACAGC TCTCATTGGC GGCTGCTCCA GTGTAGGGGA CTTTGAGAAG 
GCTTTAGAGT TGATTGACGT GATGTTAGTA AAAGGGTTCG CAACTGTTGC CACATTCAAC 
ACTTTGATTA ATGGCTTCTG CAAGCTTGGA AAGCTACAAG AAGCTAGTAA GTTATTAGAT 
CTAATGGTGG AAAAGCAAAT ACTGCCTAAT TTTGCTACCT ATTCAACTTT ATTTGCGGGA 
TTTGACAGGG CCGAAAAGGT AGACGAAGCA TTTCAGTTAT TTATTCAGAT AGAGGAGAAG 
AACCTTAGAC CGAATGCTGT CATTTATATG TCTGAATTAT TTAAAGATAT GGTAAATAAA 
GGCATATTGT CTGATAAAAT TACTGATAGA ATATTTCAGT TGCATTGCAA AGAAGGAAAT 
GTGGTCGAGG CCTTAAAATT GCGTGAAGCT ATTATTGCAG GAGGCACAAA AATTAGCAGT 
GCAGCATATT TTTTATTGGT GGATGGCCTT TGTAGGATGG GGAACTTACC TGAAGCAATT 
GAGTTATTTA ATGAAATGAA AGAACAAGAA ATCAAGCCTG ACGATGCACA ATGCCTGATG 
CTACTCCGTC GTCTTTACAC AAATGGGTTT ATTGATGAAT CCGATAAGAT TTTGGGAATT 
TTATTATGCA ATAATTGGTT GCAAGAAGGC ACTAAATTGA GCGATATGGG AGAGGAGGAT 
CAGGACATTG TGAATGGCGA GTCGGTGATT AAGGTGGAAG GTGTGCTTAA GGCATGA