LOC109712628 cDNA ORF clone, Ananas comosus(pineapple)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109712628
Entrez Gene ID	109712628
Full Name	E3 ubiquitin-protein ligase At3g02290-like
Gene Type	protein-coding
Organism	Ananas comosus(pineapple)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020236305.1	XP_020091894.1	E3 ubiquitin-protein ligase At3g02290-like isoform X1
XM_020236306.1	XP_020091895.1	E3 ubiquitin-protein ligase At3g02290-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC109712628 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109712628 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAn67594	XM_020236305.1 Latest version!	Ananas comosus E3 ubiquitin-protein ligase At3g02290-like (LOC109712628), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OAn67595	XM_020236306.1 Latest version!	Ananas comosus E3 ubiquitin-protein ligase At3g02290-like (LOC109712628), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109712628 ( XM_020236305.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020091894.1 Ananas comosus E3 ubiquitin-protein ligase At3g02290-like (LOC109712628), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAn67594
Clone ID Related Accession (Same CDS sequence)	XM_020236305.1
Accession Version	XM_020236305.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	E3 ubiquitin-protein ligase At3g02290-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033627.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCT TTCTTTGTTG CCTGCGTGTG GACGATCCAG AAGAGGATCG GCATCCAACT 
GCTTCCGTCG ACGGCGGCTG TTTAGGCCTC ATCAGATGGT CCTCTCTACC GTTGCTGAAT 
GTGTATGTTG GATTATTTAA CAGAGGTCAA GCACGCATCG CCCCTTCACC TGAACAGGGA 
ACAAATTCTT CATCTTCCAC CTCTCAACAG CAAGACCGTT CCTACATGGA CACATTCCGA 
TGTCCTCTGC GGCCCCTAGC TTATGATGAT CCAAGATGCT CTCAGGTGCG GCGTCGAAGT 
GATGGGAAAG TGGCTGCCAA AGCCTCGAGT CATTGCCATG CTGCGTCTGA ACCAACGAGA 
CATGGCAATG ATGCTTGTGT AGAATCAACA GCTGCAATGG AAAAGTTGAA TGAAATTTTA 
CTTAAGAGTT CATTAGCAGA CGGACTGAGT GAGGAAGCAG ATGCATATTC TTCATCTGAA 
GATGAGGATG ATTGTCCGAT TTGCCTTGAA GAATACGTTT CTGAGAACCC GAAGATGATA 
TTGCAATGCA CTCACCATTA CCACCTAAGT TGTATATATG AGTGGATGGA AAGAAGTGAA 
GCATGTCCGG TCTGTGGCAA GGTAATGTTG TTCAATGAGG CAACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020091894.1
CDS	443..1090
Translation	MGSFLCCLRVDDPEEDRHPTASVDGGCLGLIRWSSLPLLNVYVGLFNRGQARIAPSPEQGTNSSSSTSQQQDRSYMDTFRCPLRPLAYDDPRCSQVRRRSDGKVAAKASSHCHAASEPTRHGNDACVESTAAMEKLNEILLKSSLADGLSEEADAYSSSEDEDDCPICLEEYVSENPKMILQCTHHYHLSCIYEWMERSEACPVCGKVMLFNEAT

Target ORF information:

RefSeq Version	XM_020236305.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus E3 ubiquitin-protein ligase At3g02290-like (LOC109712628), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020236305.1

ORF Insert Sequence:

ATGGGTTCCT TTCTTTGTTG CCTGCGTGTG GACGATCCAG AAGAGGATCG GCATCCAACT 
GCTTCCGTCG ACGGCGGCTG TTTAGGCCTC ATCAGATGGT CCTCTCTACC GTTGCTGAAT 
GTGTATGTTG GATTATTTAA CAGAGGTCAA GCACGCATCG CCCCTTCACC TGAACAGGGA 
ACAAATTCTT CATCTTCCAC CTCTCAACAG CAAGACCGTT CCTACATGGA CACATTCCGA 
TGTCCTCTGC GGCCCCTAGC TTATGATGAT CCAAGATGCT CTCAGGTGCG GCGTCGAAGT 
GATGGGAAAG TGGCTGCCAA AGCCTCGAGT CATTGCCATG CTGCGTCTGA ACCAACGAGA 
CATGGCAATG ATGCTTGTGT AGAATCAACA GCTGCAATGG AAAAGTTGAA TGAAATTTTA 
CTTAAGAGTT CATTAGCAGA CGGACTGAGT GAGGAAGCAG ATGCATATTC TTCATCTGAA 
GATGAGGATG ATTGTCCGAT TTGCCTTGAA GAATACGTTT CTGAGAACCC GAAGATGATA 
TTGCAATGCA CTCACCATTA CCACCTAAGT TGTATATATG AGTGGATGGA AAGAAGTGAA 
GCATGTCCGG TCTGTGGCAA GGTAATGTTG TTCAATGAGG CAACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109712628 ( XM_020236306.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020091895.1 Ananas comosus E3 ubiquitin-protein ligase At3g02290-like (LOC109712628), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAn67595
Clone ID Related Accession (Same CDS sequence)	XM_020236306.1
Accession Version	XM_020236306.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	E3 ubiquitin-protein ligase At3g02290-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033627.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACAT TCCGATGTCC TCTGCGGCCC CTAGCTTATG ATGATCCAAG ATGCTCTCAG 
GTGCGGCGTC GAAGTGATGG GAAAGTGGCT GCCAAAGCCT CGAGTCATTG CCATGCTGCG 
TCTGAACCAA CGAGACATGG CAATGATGCT TGTGTAGAAT CAACAGCTGC AATGGAAAAG 
TTGAATGAAA TTTTACTTAA GAGTTCATTA GCAGACGGAC TGAGTGAGGA AGCAGATGCA 
TATTCTTCAT CTGAAGATGA GGATGATTGT CCGATTTGCC TTGAAGAATA CGTTTCTGAG 
AACCCGAAGA TGATATTGCA ATGCACTCAC CATTACCACC TAAGTTGTAT ATATGAGTGG 
ATGGAAAGAA GTGAAGCATG TCCGGTCTGT GGCAAGGTAA TGTTGTTCAA TGAGGCAACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020091895.1
CDS	219..641
Translation	MDTFRCPLRPLAYDDPRCSQVRRRSDGKVAAKASSHCHAASEPTRHGNDACVESTAAMEKLNEILLKSSLADGLSEEADAYSSSEDEDDCPICLEEYVSENPKMILQCTHHYHLSCIYEWMERSEACPVCGKVMLFNEAT

Target ORF information:

RefSeq Version	XM_020236306.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus E3 ubiquitin-protein ligase At3g02290-like (LOC109712628), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020236306.1

ORF Insert Sequence:

ATGGACACAT TCCGATGTCC TCTGCGGCCC CTAGCTTATG ATGATCCAAG ATGCTCTCAG 
GTGCGGCGTC GAAGTGATGG GAAAGTGGCT GCCAAAGCCT CGAGTCATTG CCATGCTGCG 
TCTGAACCAA CGAGACATGG CAATGATGCT TGTGTAGAAT CAACAGCTGC AATGGAAAAG 
TTGAATGAAA TTTTACTTAA GAGTTCATTA GCAGACGGAC TGAGTGAGGA AGCAGATGCA 
TATTCTTCAT CTGAAGATGA GGATGATTGT CCGATTTGCC TTGAAGAATA CGTTTCTGAG 
AACCCGAAGA TGATATTGCA ATGCACTCAC CATTACCACC TAAGTTGTAT ATATGAGTGG 
ATGGAAAGAA GTGAAGCATG TCCGGTCTGT GGCAAGGTAA TGTTGTTCAA TGAGGCAACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.