LOC111580733 cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC111580733
Entrez Gene ID	111580733
Full Name	myosin heavy chain, fast skeletal muscle-like
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023288601.1	XP_023144369.1	myosin heavy chain, fast skeletal muscle-like
XM_023288603.1	XP_023144371.1	myosin heavy chain, fast skeletal muscle-like

Pathways from BioSystems
Gene Ontology

The following LOC111580733 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC111580733 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm92329	XM_023288601.1 Latest version!	Amphiprion ocellaris myosin heavy chain, fast skeletal muscle-like (LOC111580733), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAm92329	XM_023288603.1 Latest version!	Amphiprion ocellaris myosin heavy chain, fast skeletal muscle-like (LOC111580733), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC111580733 ( XM_023288601.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023144369.1 Amphiprion ocellaris myosin heavy chain, fast skeletal muscle-like (LOC111580733), transcript variant X1, mRNA.

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CloneID	OAm92329
Clone ID Related Accession (Same CDS sequence)	XM_023288601.1 , XM_023288603.1
Accession Version	XM_023288601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	myosin heavy chain, fast skeletal muscle-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019373450.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACAG ACGCGGAGAT GGAGCAGTAT GGCCCGGCGG CCATTTACCT CCGGAAGCCA 
GAAAGGGAGA GGATTGAGGC CCAGACTGCT CCATTTGATG CCAAAACAGC CTTCTTTGTG 
CCCGATAATG ATGAGATGTA TCTCAAGGGT AAACTAGTCA AAAAGGAGGG TGGCAAAGCC 
ACAGTTGAGA CAGATGGGGG AAAGAGTATC ACTGTAAAAG AGGATGAGAT CCATCCCAGG 
AATCCTCCAA AGTTCGATAA GATGGAGGAC ATGGCCATGA TGACCCACCT CAACGAGCCA 
TCTGTGTTGT ATAACCTCAA AGAGCGTTAC GCCTCATGGA TGATCTATAC CTACTCTGGG 
TTGTTCTGCG TTGTCGTAAA TCCCTACAAA TGGCTTCCCG TGTATGACGC TACATGTGTA 
GCAGCATACA GAGGCAAAAA GAGGATTGAG GCTCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTCATGCT CACTGATCGT GAGAACCAGT CTGTCCTGAT CACCGGAGAA 
TCTGGTGCTG GAAAGACTGT CAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
TCTCTGGGAG CTAAGAAGGC TGAGGCAACA GCTGGCAAGA TGCAGGGCTC TCTTGAAGAC 
CAGATTGTTG CAGCCAACCC TCTGCTGGAG GCTTATGGTA ATGCCAAGAC TGTGAGGAAT 
GACAACTCCT CTCGTTTTGG TAAATTCATC AGGATCCACT TCGGCTCCTC TGGCAAGCTG 
GCTTCAGCTG ATATTGAAAC ATATCTGCTG GAGAAGTCTC GTGTCACCTT CCAGTTGTCT 
GCTGAGAGGA GCTACCATAT CTTCTATCAG CTGATGACTG GACACAAGCC TGAGCTCCTG 
GAGGCTCTTC TGATCACCAC TAACCCCTAT GACTATCCAA TGATCAGTCA GGGTGAAATC 
ACTGTCAAGA GCATCAATGA TGTGGAGGAG TTCATTGCAA CAGATACTGC TATTGACATC 
TTGGGTTTCA ACGCTGAAGA GAAAATCAAC ATCTACAAGC TGACCGGTGC TGTGATGCAT 
CATGGCAACA TGAAATTCAA GCAAAAGCAG AGAGAGGAGC AGGCTGAGCC TGATGGCACT 
GAGGTGGCTG ATAAAATCGC CTATCTCTTC GGCCTGAACT CAGCTGATAT GCTGAAAGCT 
CTGTGCTACC CAAGAGTCAA AGTCGGCAAT GAGATGGTCA CCAAAGGTCA GACTGTCCCA 
CAGGTCAACA ATGCTGTCAG TGCTCTGTGC AAGTCTGTCT ATGAGAAAAT GTTCTTGTGG 
ATGGTCATCC GTATCAATGA GATGCTGGAC ACAAAGCAGC CAAGACAGTT CTTCATTGGT 
GTGTTGGACA TCGCTGGATT TGAGATCTTT GATTTCAACA GCTTGGAGCA ACTCTGCATT 
AACTTCACCA ATGAGAAACT GCAACAGTTC TTCAACCACA CCATGTTTGT CCTGGAGCAA 
GAGGAGTACA AGAAAGAAGG TATTGAATGG GAGTTCATTG ACTTCGGTAT GGACTTGGCT 
GCCTGCATTG AGCTTATCGA GAAGCCAATG GGCATCTTCT CCATCCTTGA AGAGGAGTGC 
ATGTTCCCCA AGGCCTCTGA TACAACTTTC AAGAACAAGC TGCATGATCA GCATCTTGGC 
AAGACCAAGG CCTTTGAGAA GCCAAAGCCT GGAAAGGGCA AAGCTGAGGC TCACTTCTCC 
CTGGTTCACT ATGCTGGTAC AGTGGACTAC AATATTGTTG GCTGGCTGGA CAAGAACAAG 
GACCCACTGA ATGAATCTGT TGTTCAGCTC TACCAGAAGT CTGCAAACAA ACTGCTGGTC 
TACCTTTATG CATCCCATGC CTCAGCTGAG GAGGCTTCTG GTGGTGGTGG CAAGAAGGGT 
GGCAAGAAGA AGGGTGGCTC CTTCCAGACT GTGTCCGCTC TTTTCAGAGA GAACTTGGGC 
AAACTGATGA CCAACTTGAG GAGCACCCAT CCTCACTTTG TCCGTTGCTT GATTCCCAAT 
GAGACAAAGA CCCCAGGTCT TATGGAGAAC TTCTTGGTCA TCCACCAGCT GAGGTGTAAT 
GGTGTGCTGG AGGGCATCAG AATCTGTAGA AAGGGCTTCC CCAGCAGAAT TCTCTATGGT 
GACTTCAAGC AGAGATACAA AGTATTGAAT GCCAGTGTCA TCCCTGAGGG ACAGTTCATT 
GACAACAAGA AAGCTTCAGA GAAGCTGTTG GGCTCCATTG ATGTGGACCA CACTCAGTAC 
AAGTTTGGAC ACACTAAGGT GTTCTTCAAA GCTGGTCTGC TGGGTACCCT TGAGGAGATG 
AGAGATGAGA AACTGGCTTC ACTGGTGACC ATGACTCAAG CTCTCTGCAG AGGATATGTC 
ATGAGGAAGG AGTTTGTAAA GATGATGGAG AGGAGAGAAT CTATCTACTC CATCCAGTAC 
AATATCCGTT CATTCATGAA TGTGAAGAAC TGGCCATGGC TGAAGCTGTA CTTCAAGATC 
AAACCTCTTC TGAAGAGTGC TGAGACTGAG AAGGAGTTGC AGCAGATGAA GGAGAACTAT 
GATAAGATGC AGTCAGACCT GGCTGCTGCT TTGGCCAAGA AGAAGGAGCT GGAGGAGAAG 
ATGGTTTCCC TGCTGCAGGA GAAGAATGAC CTGCAACTCC AAGTGGCTGC AGAAGTTGAG 
AATCTCTCGG ATGCTGAGGA AAGGTGTGAG GGGCTCATTA AGAGCAAGAT CCAGCTCGAG 
GCCAAACTCA AAGAGACAAC TGAGAGACTG GAGGATGAAG AGGAAATCAA TGCTGAGCTG 
ACTGCCAAGA AGAGGAAGTT GGAGGATGAA TGCTCTGAGC TGAAGAAGGA CATTGATGAC 
TTGGAGCTCA CCTTGGCTAA AGTGGAGAAG GAGAAACATG CCACAGAAAA CAAGGTGAAA 
AACCTGACAG AGGAAATGGC CACTCAAGAT GAGTCTATTG CCAAGCTGAC CAAGGAGAAG 
AAAGCCCTCC AAGAGAGCCA TCAGCAGACA CTGGATGACC TCCAGGCAGA GGAAGACAAA 
GTCAACACTC TGACCAAGGC CAAGACAAAG CTGGAACAGC AAGTGGACGA TCTTGAGGGC 
TCACTGGAGC AGGAGAAGAA GCTCCGCATG GACCTTGAGA GAGCCAAGAG GAAGCTTGAG 
GGAGATCTGA AACTGGCCCA GGAATCCATA ATGGATCTGG AGAATGACAA GCAGCAGTCT 
GAGGAGAAAA TCAAGAAGAA GGACTTTGAG ACCAGTCAGC TCCTCAGCAA GATTGAGGAT 
GAACAGTCCC TTGGTGCTCA GCTTCAGAAG AAGATCAAGG AACTCCAGGC TCGCATTGAG 
GAGCTGGAGG AGGAGATTGA GGCTGAGAGG GCTGCTCGAG CTAAGGTAGA GAAGCAGAGA 
GCTGATCTCT CCAGGGAGCT TGAGGAGATC AGTGAGAGGC TTGAGGAGGC TGGTGGAGCA 
ACAGCTGCTC AGATTGAGAT GAACAAGAAG CGTGAGGCTG AGTTCCAGAA GTTGCGTCGT 
GACCTTGAAG AGTCAACCCT GCAGCACGAA TCTACTGCAG CAGCTCTCCG CAAGAAGCAG 
GCTGACAGCG TTGCAGAGCT GGGAGAGCAG ATCGACAACC TTCAGCGTGT CAAGCAGAAG 
CTGGAGAAGG AGAAGAGCGA GTACAAGATG GAGATTGATG ATCTCTCCAG CAACATGGAG 
GCTGTTGCCA AGGCGAAGGG CAATTTGGAG AAAATGTGCA GAACTCTTGA GGACCAGCTG 
AGTGAGTTGA AGGCCAAAAA TGACGAGAAT GTTCGCCAAC TGAACGACAT CAATGCACAG 
AGGGCCAGAC TTCAGACAGA GAATGGTGAG TTTTCTCGCC AGCTTGAGGA GAAGGAAGCT 
CTTGTTTCCC AGCTGACCAG AGGCAAGCAG GCCTTCACCC AGCAGATTGA GGAGCTCAAG 
AGGCACATTG AGGAAGAAGT GAAGGCAAAG AATGCCCTGG CCCATGGTGT TCAGTCAGCT 
CGCCATGACT GTGATCTGCT CAGAGAGCAG TTTGAGGAGG AGCAGGAGGC CAAGGCTGAG 
CTGCAGAGAG GAATGTCCAA GGCCAACAGT GAGGTGGCTC AGTGGAGAAG CAAATATGAG 
ACTGATGCTA TCCAGCGCAC TGAGGAGCTG GAGGAGGCCA AGAAAAAGCT TGCCCAGCGC 
CTGCAGGAGG CTGAGGAATC CATTGAGGCT GTGAACTCCA AGTGTGCCTC TTTGGAGAAG 
ACCAAGCAGA GGCTGCAGGG TGAGGTGGAG GACCTCATGA TTGATGTGGA GAGAGCTAAC 
GCTCTGGCTG CCAACCTTGA CAAGAAGCAG AGGAACTTTG ATAAGGTCCT TGCAGAATGG 
AAGCAGAAGT ATGAGGAGGG CCAGGCAGAA CTGGAGGGAG CACAGAAGGA GGCTCGTTCT 
CTCAGCACCG AGTTGTTCAA GATGAAGAAC TCCTATGAGG AGGCTCTGGA TCACCTAGAG 
ACCATGAAGA GGGAGAACAA GAACCTGCAA CAGGAGATCT CAGATCTGAC TGAGCAGATC 
GGTGAGACTG GAAAGAGCAT CCATGAGCTG GAAAAAGCCA AGAAGACTGT TGAGACTGAG 
AAATCTGAAA TCCAGTCTGC ACTGGAGGAA GCTGAGGGTA CTCTGGAGCA TGAGGAGGCC 
AAGATTCTCC GTGTTCAGCT TGAGCTGAAC CAGATCAAAG GTGAGGTCGA CAGGAAGCTG 
GCAGAGAAGG ATGAGGAGAT GGAACAGATC AAGAGGAACA GCCAGAGGGT GATAGACTCC 
ATGCAGAGCA ATCTTGATGC TGAGGTCAGG AGCAGGAATG ATGCCCTGAG AGTCAAGAAG 
AAGATGGAGG GAGACCTGAA TGAGATGGAG ATTCAGCTGA GCCATGCCAA CAGGCAGGCT 
TCTGAGGCCC AGAAACAACT GAGGAACGTC CAGGGACAGC TCAAGGATGC TCAACTGCAC 
CTTGATGATG CCGTCAGAGG ACAGGAAGAC ATGAAGGAGC AGGTTGCCAT GGTGGAGCGC 
AGGAATGGTC TGATGATGGC TGAGATTGAA GAGCTGAGAG CTGCTCTGGA GCAGACCGAG 
AGAGGACGCA AAGTGGCTGA GCAGGAGTTG GTTGATGCTA GTGAGCGTGT CGGCCTGCTT 
CACTCCCAGA ACACCAGCCT TCTGAACACC AAGAAGAAGC TGGAGTCTGA CCTCGTCCAG 
GTCCAGGGTG AAGTGGATGA TGCTGTTCAG GAAGCAAGAA ATGCTGAGGA TAAAGCCAAA 
AAGGCTATCA CTGATGCTGC CATGATGGCT GAGGAGCTGA AGAAGGAGCA GGACACCAGC 
GCTCACCTGG AGAGGATGAA GAAGAACCTG GAGGTCACAG TCAAGGACCT GCAGCACCGT 
CTGGATGAGG CTGAGAACCT GGCCATGAAG GGTGGCAAGA AGCAGCTTCA GAAACTGGAA 
TCCAGGGTGC GTGAGCTTGA AACTGAAGTT GAAGGTGAGC AGAGACGTGG AGCTGATGCT 
GTCAAAGGAG TGCGCAAATA TGAGAGGAGA GTGAAGGAGC TGACCTACCA GACTGAGGAG 
GACAAGAAGA ACATGACCAG ACTTCAGGAT CTGGTGGATA AGCTGCAGCT CAAAGTCAAA 
GCTTACAAGA GACAGTCTGA GGAGGCTGAG GAACAGGCCA ACACGCACAT GTCCAGGCTC 
AGAAAGGTTC AGCATGAGCT GGAGGAAGCT CAGGAGCGTG CTGACATTGC TGAGTCCCAG 
GTCAACAAGC TGAGAGCCAA GAGCCGTGAT GCTGGAAAGA GTGAGTCTGC TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023144369.1
CDS	86..5902
Translation	MSTDAEMEQYGPAAIYLRKPERERIEAQTAPFDAKTAFFVPDNDEMYLKGKLVKKEGGKATVETDGGKSITVKEDEIHPRNPPKFDKMEDMAMMTHLNEPSVLYNLKERYASWMIYTYSGLFCVVVNPYKWLPVYDATCVAAYRGKKRIEAPPHIFSISDNAYQFMLTDRENQSVLITGESGAGKTVNTKRVIQYFATIASLGAKKAEATAGKMQGSLEDQIVAANPLLEAYGNAKTVRNDNSSRFGKFIRIHFGSSGKLASADIETYLLEKSRVTFQLSAERSYHIFYQLMTGHKPELLEALLITTNPYDYPMISQGEITVKSINDVEEFIATDTAIDILGFNAEEKINIYKLTGAVMHHGNMKFKQKQREEQAEPDGTEVADKIAYLFGLNSADMLKALCYPRVKVGNEMVTKGQTVPQVNNAVSALCKSVYEKMFLWMVIRINEMLDTKQPRQFFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHTMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKASDTTFKNKLHDQHLGKTKAFEKPKPGKGKAEAHFSLVHYAGTVDYNIVGWLDKNKDPLNESVVQLYQKSANKLLVYLYASHASAEEASGGGGKKGGKKKGGSFQTVSALFRENLGKLMTNLRSTHPHFVRCLIPNETKTPGLMENFLVIHQLRCNGVLEGIRICRKGFPSRILYGDFKQRYKVLNASVIPEGQFIDNKKASEKLLGSIDVDHTQYKFGHTKVFFKAGLLGTLEEMRDEKLASLVTMTQALCRGYVMRKEFVKMMERRESIYSIQYNIRSFMNVKNWPWLKLYFKIKPLLKSAETEKELQQMKENYDKMQSDLAAALAKKKELEEKMVSLLQEKNDLQLQVAAEVENLSDAEERCEGLIKSKIQLEAKLKETTERLEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMATQDESIAKLTKEKKALQESHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDLENDKQQSEEKIKKKDFETSQLLSKIEDEQSLGAQLQKKIKELQARIEELEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKLRRDLEESTLQHESTAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSEYKMEIDDLSSNMEAVAKAKGNLEKMCRTLEDQLSELKAKNDENVRQLNDINAQRARLQTENGEFSRQLEEKEALVSQLTRGKQAFTQQIEELKRHIEEEVKAKNALAHGVQSARHDCDLLREQFEEEQEAKAELQRGMSKANSEVAQWRSKYETDAIQRTEELEEAKKKLAQRLQEAEESIEAVNSKCASLEKTKQRLQGEVEDLMIDVERANALAANLDKKQRNFDKVLAEWKQKYEEGQAELEGAQKEARSLSTELFKMKNSYEEALDHLETMKRENKNLQQEISDLTEQIGETGKSIHELEKAKKTVETEKSEIQSALEEAEGTLEHEEAKILRVQLELNQIKGEVDRKLAEKDEEMEQIKRNSQRVIDSMQSNLDAEVRSRNDALRVKKKMEGDLNEMEIQLSHANRQASEAQKQLRNVQGQLKDAQLHLDDAVRGQEDMKEQVAMVERRNGLMMAEIEELRAALEQTERGRKVAEQELVDASERVGLLHSQNTSLLNTKKKLESDLVQVQGEVDDAVQEARNAEDKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEVTVKDLQHRLDEAENLAMKGGKKQLQKLESRVRELETEVEGEQRRGADAVKGVRKYERRVKELTYQTEEDKKNMTRLQDLVDKLQLKVKAYKRQSEEAEEQANTHMSRLRKVQHELEEAQERADIAESQVNKLRAKSRDAGKSESAE

Target ORF information:

RefSeq Version	XM_023288601.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris myosin heavy chain, fast skeletal muscle-like (LOC111580733), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023288601.1

ORF Insert Sequence:

ATGAGCACAG ACGCGGAGAT GGAGCAGTAT GGCCCGGCGG CCATTTACCT CCGGAAGCCA 
GAAAGGGAGA GGATTGAGGC CCAGACTGCT CCATTTGATG CCAAAACAGC CTTCTTTGTG 
CCCGATAATG ATGAGATGTA TCTCAAGGGT AAACTAGTCA AAAAGGAGGG TGGCAAAGCC 
ACAGTTGAGA CAGATGGGGG AAAGAGTATC ACTGTAAAAG AGGATGAGAT CCATCCCAGG 
AATCCTCCAA AGTTCGATAA GATGGAGGAC ATGGCCATGA TGACCCACCT CAACGAGCCA 
TCTGTGTTGT ATAACCTCAA AGAGCGTTAC GCCTCATGGA TGATCTATAC CTACTCTGGG 
TTGTTCTGCG TTGTCGTAAA TCCCTACAAA TGGCTTCCCG TGTATGACGC TACATGTGTA 
GCAGCATACA GAGGCAAAAA GAGGATTGAG GCTCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTCATGCT CACTGATCGT GAGAACCAGT CTGTCCTGAT CACCGGAGAA 
TCTGGTGCTG GAAAGACTGT CAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
TCTCTGGGAG CTAAGAAGGC TGAGGCAACA GCTGGCAAGA TGCAGGGCTC TCTTGAAGAC 
CAGATTGTTG CAGCCAACCC TCTGCTGGAG GCTTATGGTA ATGCCAAGAC TGTGAGGAAT 
GACAACTCCT CTCGTTTTGG TAAATTCATC AGGATCCACT TCGGCTCCTC TGGCAAGCTG 
GCTTCAGCTG ATATTGAAAC ATATCTGCTG GAGAAGTCTC GTGTCACCTT CCAGTTGTCT 
GCTGAGAGGA GCTACCATAT CTTCTATCAG CTGATGACTG GACACAAGCC TGAGCTCCTG 
GAGGCTCTTC TGATCACCAC TAACCCCTAT GACTATCCAA TGATCAGTCA GGGTGAAATC 
ACTGTCAAGA GCATCAATGA TGTGGAGGAG TTCATTGCAA CAGATACTGC TATTGACATC 
TTGGGTTTCA ACGCTGAAGA GAAAATCAAC ATCTACAAGC TGACCGGTGC TGTGATGCAT 
CATGGCAACA TGAAATTCAA GCAAAAGCAG AGAGAGGAGC AGGCTGAGCC TGATGGCACT 
GAGGTGGCTG ATAAAATCGC CTATCTCTTC GGCCTGAACT CAGCTGATAT GCTGAAAGCT 
CTGTGCTACC CAAGAGTCAA AGTCGGCAAT GAGATGGTCA CCAAAGGTCA GACTGTCCCA 
CAGGTCAACA ATGCTGTCAG TGCTCTGTGC AAGTCTGTCT ATGAGAAAAT GTTCTTGTGG 
ATGGTCATCC GTATCAATGA GATGCTGGAC ACAAAGCAGC CAAGACAGTT CTTCATTGGT 
GTGTTGGACA TCGCTGGATT TGAGATCTTT GATTTCAACA GCTTGGAGCA ACTCTGCATT 
AACTTCACCA ATGAGAAACT GCAACAGTTC TTCAACCACA CCATGTTTGT CCTGGAGCAA 
GAGGAGTACA AGAAAGAAGG TATTGAATGG GAGTTCATTG ACTTCGGTAT GGACTTGGCT 
GCCTGCATTG AGCTTATCGA GAAGCCAATG GGCATCTTCT CCATCCTTGA AGAGGAGTGC 
ATGTTCCCCA AGGCCTCTGA TACAACTTTC AAGAACAAGC TGCATGATCA GCATCTTGGC 
AAGACCAAGG CCTTTGAGAA GCCAAAGCCT GGAAAGGGCA AAGCTGAGGC TCACTTCTCC 
CTGGTTCACT ATGCTGGTAC AGTGGACTAC AATATTGTTG GCTGGCTGGA CAAGAACAAG 
GACCCACTGA ATGAATCTGT TGTTCAGCTC TACCAGAAGT CTGCAAACAA ACTGCTGGTC 
TACCTTTATG CATCCCATGC CTCAGCTGAG GAGGCTTCTG GTGGTGGTGG CAAGAAGGGT 
GGCAAGAAGA AGGGTGGCTC CTTCCAGACT GTGTCCGCTC TTTTCAGAGA GAACTTGGGC 
AAACTGATGA CCAACTTGAG GAGCACCCAT CCTCACTTTG TCCGTTGCTT GATTCCCAAT 
GAGACAAAGA CCCCAGGTCT TATGGAGAAC TTCTTGGTCA TCCACCAGCT GAGGTGTAAT 
GGTGTGCTGG AGGGCATCAG AATCTGTAGA AAGGGCTTCC CCAGCAGAAT TCTCTATGGT 
GACTTCAAGC AGAGATACAA AGTATTGAAT GCCAGTGTCA TCCCTGAGGG ACAGTTCATT 
GACAACAAGA AAGCTTCAGA GAAGCTGTTG GGCTCCATTG ATGTGGACCA CACTCAGTAC 
AAGTTTGGAC ACACTAAGGT GTTCTTCAAA GCTGGTCTGC TGGGTACCCT TGAGGAGATG 
AGAGATGAGA AACTGGCTTC ACTGGTGACC ATGACTCAAG CTCTCTGCAG AGGATATGTC 
ATGAGGAAGG AGTTTGTAAA GATGATGGAG AGGAGAGAAT CTATCTACTC CATCCAGTAC 
AATATCCGTT CATTCATGAA TGTGAAGAAC TGGCCATGGC TGAAGCTGTA CTTCAAGATC 
AAACCTCTTC TGAAGAGTGC TGAGACTGAG AAGGAGTTGC AGCAGATGAA GGAGAACTAT 
GATAAGATGC AGTCAGACCT GGCTGCTGCT TTGGCCAAGA AGAAGGAGCT GGAGGAGAAG 
ATGGTTTCCC TGCTGCAGGA GAAGAATGAC CTGCAACTCC AAGTGGCTGC AGAAGTTGAG 
AATCTCTCGG ATGCTGAGGA AAGGTGTGAG GGGCTCATTA AGAGCAAGAT CCAGCTCGAG 
GCCAAACTCA AAGAGACAAC TGAGAGACTG GAGGATGAAG AGGAAATCAA TGCTGAGCTG 
ACTGCCAAGA AGAGGAAGTT GGAGGATGAA TGCTCTGAGC TGAAGAAGGA CATTGATGAC 
TTGGAGCTCA CCTTGGCTAA AGTGGAGAAG GAGAAACATG CCACAGAAAA CAAGGTGAAA 
AACCTGACAG AGGAAATGGC CACTCAAGAT GAGTCTATTG CCAAGCTGAC CAAGGAGAAG 
AAAGCCCTCC AAGAGAGCCA TCAGCAGACA CTGGATGACC TCCAGGCAGA GGAAGACAAA 
GTCAACACTC TGACCAAGGC CAAGACAAAG CTGGAACAGC AAGTGGACGA TCTTGAGGGC 
TCACTGGAGC AGGAGAAGAA GCTCCGCATG GACCTTGAGA GAGCCAAGAG GAAGCTTGAG 
GGAGATCTGA AACTGGCCCA GGAATCCATA ATGGATCTGG AGAATGACAA GCAGCAGTCT 
GAGGAGAAAA TCAAGAAGAA GGACTTTGAG ACCAGTCAGC TCCTCAGCAA GATTGAGGAT 
GAACAGTCCC TTGGTGCTCA GCTTCAGAAG AAGATCAAGG AACTCCAGGC TCGCATTGAG 
GAGCTGGAGG AGGAGATTGA GGCTGAGAGG GCTGCTCGAG CTAAGGTAGA GAAGCAGAGA 
GCTGATCTCT CCAGGGAGCT TGAGGAGATC AGTGAGAGGC TTGAGGAGGC TGGTGGAGCA 
ACAGCTGCTC AGATTGAGAT GAACAAGAAG CGTGAGGCTG AGTTCCAGAA GTTGCGTCGT 
GACCTTGAAG AGTCAACCCT GCAGCACGAA TCTACTGCAG CAGCTCTCCG CAAGAAGCAG 
GCTGACAGCG TTGCAGAGCT GGGAGAGCAG ATCGACAACC TTCAGCGTGT CAAGCAGAAG 
CTGGAGAAGG AGAAGAGCGA GTACAAGATG GAGATTGATG ATCTCTCCAG CAACATGGAG 
GCTGTTGCCA AGGCGAAGGG CAATTTGGAG AAAATGTGCA GAACTCTTGA GGACCAGCTG 
AGTGAGTTGA AGGCCAAAAA TGACGAGAAT GTTCGCCAAC TGAACGACAT CAATGCACAG 
AGGGCCAGAC TTCAGACAGA GAATGGTGAG TTTTCTCGCC AGCTTGAGGA GAAGGAAGCT 
CTTGTTTCCC AGCTGACCAG AGGCAAGCAG GCCTTCACCC AGCAGATTGA GGAGCTCAAG 
AGGCACATTG AGGAAGAAGT GAAGGCAAAG AATGCCCTGG CCCATGGTGT TCAGTCAGCT 
CGCCATGACT GTGATCTGCT CAGAGAGCAG TTTGAGGAGG AGCAGGAGGC CAAGGCTGAG 
CTGCAGAGAG GAATGTCCAA GGCCAACAGT GAGGTGGCTC AGTGGAGAAG CAAATATGAG 
ACTGATGCTA TCCAGCGCAC TGAGGAGCTG GAGGAGGCCA AGAAAAAGCT TGCCCAGCGC 
CTGCAGGAGG CTGAGGAATC CATTGAGGCT GTGAACTCCA AGTGTGCCTC TTTGGAGAAG 
ACCAAGCAGA GGCTGCAGGG TGAGGTGGAG GACCTCATGA TTGATGTGGA GAGAGCTAAC 
GCTCTGGCTG CCAACCTTGA CAAGAAGCAG AGGAACTTTG ATAAGGTCCT TGCAGAATGG 
AAGCAGAAGT ATGAGGAGGG CCAGGCAGAA CTGGAGGGAG CACAGAAGGA GGCTCGTTCT 
CTCAGCACCG AGTTGTTCAA GATGAAGAAC TCCTATGAGG AGGCTCTGGA TCACCTAGAG 
ACCATGAAGA GGGAGAACAA GAACCTGCAA CAGGAGATCT CAGATCTGAC TGAGCAGATC 
GGTGAGACTG GAAAGAGCAT CCATGAGCTG GAAAAAGCCA AGAAGACTGT TGAGACTGAG 
AAATCTGAAA TCCAGTCTGC ACTGGAGGAA GCTGAGGGTA CTCTGGAGCA TGAGGAGGCC 
AAGATTCTCC GTGTTCAGCT TGAGCTGAAC CAGATCAAAG GTGAGGTCGA CAGGAAGCTG 
GCAGAGAAGG ATGAGGAGAT GGAACAGATC AAGAGGAACA GCCAGAGGGT GATAGACTCC 
ATGCAGAGCA ATCTTGATGC TGAGGTCAGG AGCAGGAATG ATGCCCTGAG AGTCAAGAAG 
AAGATGGAGG GAGACCTGAA TGAGATGGAG ATTCAGCTGA GCCATGCCAA CAGGCAGGCT 
TCTGAGGCCC AGAAACAACT GAGGAACGTC CAGGGACAGC TCAAGGATGC TCAACTGCAC 
CTTGATGATG CCGTCAGAGG ACAGGAAGAC ATGAAGGAGC AGGTTGCCAT GGTGGAGCGC 
AGGAATGGTC TGATGATGGC TGAGATTGAA GAGCTGAGAG CTGCTCTGGA GCAGACCGAG 
AGAGGACGCA AAGTGGCTGA GCAGGAGTTG GTTGATGCTA GTGAGCGTGT CGGCCTGCTT 
CACTCCCAGA ACACCAGCCT TCTGAACACC AAGAAGAAGC TGGAGTCTGA CCTCGTCCAG 
GTCCAGGGTG AAGTGGATGA TGCTGTTCAG GAAGCAAGAA ATGCTGAGGA TAAAGCCAAA 
AAGGCTATCA CTGATGCTGC CATGATGGCT GAGGAGCTGA AGAAGGAGCA GGACACCAGC 
GCTCACCTGG AGAGGATGAA GAAGAACCTG GAGGTCACAG TCAAGGACCT GCAGCACCGT 
CTGGATGAGG CTGAGAACCT GGCCATGAAG GGTGGCAAGA AGCAGCTTCA GAAACTGGAA 
TCCAGGGTGC GTGAGCTTGA AACTGAAGTT GAAGGTGAGC AGAGACGTGG AGCTGATGCT 
GTCAAAGGAG TGCGCAAATA TGAGAGGAGA GTGAAGGAGC TGACCTACCA GACTGAGGAG 
GACAAGAAGA ACATGACCAG ACTTCAGGAT CTGGTGGATA AGCTGCAGCT CAAAGTCAAA 
GCTTACAAGA GACAGTCTGA GGAGGCTGAG GAACAGGCCA ACACGCACAT GTCCAGGCTC 
AGAAAGGTTC AGCATGAGCT GGAGGAAGCT CAGGAGCGTG CTGACATTGC TGAGTCCCAG 
GTCAACAAGC TGAGAGCCAA GAGCCGTGAT GCTGGAAAGA GTGAGTCTGC TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC111580733 ( XM_023288603.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023144371.1 Amphiprion ocellaris myosin heavy chain, fast skeletal muscle-like (LOC111580733), transcript variant X2, mRNA.

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CloneID	OAm92329
Clone ID Related Accession (Same CDS sequence)	XM_023288601.1 , XM_023288603.1
Accession Version	XM_023288603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	myosin heavy chain, fast skeletal muscle-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019373450.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACAG ACGCGGAGAT GGAGCAGTAT GGCCCGGCGG CCATTTACCT CCGGAAGCCA 
GAAAGGGAGA GGATTGAGGC CCAGACTGCT CCATTTGATG CCAAAACAGC CTTCTTTGTG 
CCCGATAATG ATGAGATGTA TCTCAAGGGT AAACTAGTCA AAAAGGAGGG TGGCAAAGCC 
ACAGTTGAGA CAGATGGGGG AAAGAGTATC ACTGTAAAAG AGGATGAGAT CCATCCCAGG 
AATCCTCCAA AGTTCGATAA GATGGAGGAC ATGGCCATGA TGACCCACCT CAACGAGCCA 
TCTGTGTTGT ATAACCTCAA AGAGCGTTAC GCCTCATGGA TGATCTATAC CTACTCTGGG 
TTGTTCTGCG TTGTCGTAAA TCCCTACAAA TGGCTTCCCG TGTATGACGC TACATGTGTA 
GCAGCATACA GAGGCAAAAA GAGGATTGAG GCTCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTCATGCT CACTGATCGT GAGAACCAGT CTGTCCTGAT CACCGGAGAA 
TCTGGTGCTG GAAAGACTGT CAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
TCTCTGGGAG CTAAGAAGGC TGAGGCAACA GCTGGCAAGA TGCAGGGCTC TCTTGAAGAC 
CAGATTGTTG CAGCCAACCC TCTGCTGGAG GCTTATGGTA ATGCCAAGAC TGTGAGGAAT 
GACAACTCCT CTCGTTTTGG TAAATTCATC AGGATCCACT TCGGCTCCTC TGGCAAGCTG 
GCTTCAGCTG ATATTGAAAC ATATCTGCTG GAGAAGTCTC GTGTCACCTT CCAGTTGTCT 
GCTGAGAGGA GCTACCATAT CTTCTATCAG CTGATGACTG GACACAAGCC TGAGCTCCTG 
GAGGCTCTTC TGATCACCAC TAACCCCTAT GACTATCCAA TGATCAGTCA GGGTGAAATC 
ACTGTCAAGA GCATCAATGA TGTGGAGGAG TTCATTGCAA CAGATACTGC TATTGACATC 
TTGGGTTTCA ACGCTGAAGA GAAAATCAAC ATCTACAAGC TGACCGGTGC TGTGATGCAT 
CATGGCAACA TGAAATTCAA GCAAAAGCAG AGAGAGGAGC AGGCTGAGCC TGATGGCACT 
GAGGTGGCTG ATAAAATCGC CTATCTCTTC GGCCTGAACT CAGCTGATAT GCTGAAAGCT 
CTGTGCTACC CAAGAGTCAA AGTCGGCAAT GAGATGGTCA CCAAAGGTCA GACTGTCCCA 
CAGGTCAACA ATGCTGTCAG TGCTCTGTGC AAGTCTGTCT ATGAGAAAAT GTTCTTGTGG 
ATGGTCATCC GTATCAATGA GATGCTGGAC ACAAAGCAGC CAAGACAGTT CTTCATTGGT 
GTGTTGGACA TCGCTGGATT TGAGATCTTT GATTTCAACA GCTTGGAGCA ACTCTGCATT 
AACTTCACCA ATGAGAAACT GCAACAGTTC TTCAACCACA CCATGTTTGT CCTGGAGCAA 
GAGGAGTACA AGAAAGAAGG TATTGAATGG GAGTTCATTG ACTTCGGTAT GGACTTGGCT 
GCCTGCATTG AGCTTATCGA GAAGCCAATG GGCATCTTCT CCATCCTTGA AGAGGAGTGC 
ATGTTCCCCA AGGCCTCTGA TACAACTTTC AAGAACAAGC TGCATGATCA GCATCTTGGC 
AAGACCAAGG CCTTTGAGAA GCCAAAGCCT GGAAAGGGCA AAGCTGAGGC TCACTTCTCC 
CTGGTTCACT ATGCTGGTAC AGTGGACTAC AATATTGTTG GCTGGCTGGA CAAGAACAAG 
GACCCACTGA ATGAATCTGT TGTTCAGCTC TACCAGAAGT CTGCAAACAA ACTGCTGGTC 
TACCTTTATG CATCCCATGC CTCAGCTGAG GAGGCTTCTG GTGGTGGTGG CAAGAAGGGT 
GGCAAGAAGA AGGGTGGCTC CTTCCAGACT GTGTCCGCTC TTTTCAGAGA GAACTTGGGC 
AAACTGATGA CCAACTTGAG GAGCACCCAT CCTCACTTTG TCCGTTGCTT GATTCCCAAT 
GAGACAAAGA CCCCAGGTCT TATGGAGAAC TTCTTGGTCA TCCACCAGCT GAGGTGTAAT 
GGTGTGCTGG AGGGCATCAG AATCTGTAGA AAGGGCTTCC CCAGCAGAAT TCTCTATGGT 
GACTTCAAGC AGAGATACAA AGTATTGAAT GCCAGTGTCA TCCCTGAGGG ACAGTTCATT 
GACAACAAGA AAGCTTCAGA GAAGCTGTTG GGCTCCATTG ATGTGGACCA CACTCAGTAC 
AAGTTTGGAC ACACTAAGGT GTTCTTCAAA GCTGGTCTGC TGGGTACCCT TGAGGAGATG 
AGAGATGAGA AACTGGCTTC ACTGGTGACC ATGACTCAAG CTCTCTGCAG AGGATATGTC 
ATGAGGAAGG AGTTTGTAAA GATGATGGAG AGGAGAGAAT CTATCTACTC CATCCAGTAC 
AATATCCGTT CATTCATGAA TGTGAAGAAC TGGCCATGGC TGAAGCTGTA CTTCAAGATC 
AAACCTCTTC TGAAGAGTGC TGAGACTGAG AAGGAGTTGC AGCAGATGAA GGAGAACTAT 
GATAAGATGC AGTCAGACCT GGCTGCTGCT TTGGCCAAGA AGAAGGAGCT GGAGGAGAAG 
ATGGTTTCCC TGCTGCAGGA GAAGAATGAC CTGCAACTCC AAGTGGCTGC AGAAGTTGAG 
AATCTCTCGG ATGCTGAGGA AAGGTGTGAG GGGCTCATTA AGAGCAAGAT CCAGCTCGAG 
GCCAAACTCA AAGAGACAAC TGAGAGACTG GAGGATGAAG AGGAAATCAA TGCTGAGCTG 
ACTGCCAAGA AGAGGAAGTT GGAGGATGAA TGCTCTGAGC TGAAGAAGGA CATTGATGAC 
TTGGAGCTCA CCTTGGCTAA AGTGGAGAAG GAGAAACATG CCACAGAAAA CAAGGTGAAA 
AACCTGACAG AGGAAATGGC CACTCAAGAT GAGTCTATTG CCAAGCTGAC CAAGGAGAAG 
AAAGCCCTCC AAGAGAGCCA TCAGCAGACA CTGGATGACC TCCAGGCAGA GGAAGACAAA 
GTCAACACTC TGACCAAGGC CAAGACAAAG CTGGAACAGC AAGTGGACGA TCTTGAGGGC 
TCACTGGAGC AGGAGAAGAA GCTCCGCATG GACCTTGAGA GAGCCAAGAG GAAGCTTGAG 
GGAGATCTGA AACTGGCCCA GGAATCCATA ATGGATCTGG AGAATGACAA GCAGCAGTCT 
GAGGAGAAAA TCAAGAAGAA GGACTTTGAG ACCAGTCAGC TCCTCAGCAA GATTGAGGAT 
GAACAGTCCC TTGGTGCTCA GCTTCAGAAG AAGATCAAGG AACTCCAGGC TCGCATTGAG 
GAGCTGGAGG AGGAGATTGA GGCTGAGAGG GCTGCTCGAG CTAAGGTAGA GAAGCAGAGA 
GCTGATCTCT CCAGGGAGCT TGAGGAGATC AGTGAGAGGC TTGAGGAGGC TGGTGGAGCA 
ACAGCTGCTC AGATTGAGAT GAACAAGAAG CGTGAGGCTG AGTTCCAGAA GTTGCGTCGT 
GACCTTGAAG AGTCAACCCT GCAGCACGAA TCTACTGCAG CAGCTCTCCG CAAGAAGCAG 
GCTGACAGCG TTGCAGAGCT GGGAGAGCAG ATCGACAACC TTCAGCGTGT CAAGCAGAAG 
CTGGAGAAGG AGAAGAGCGA GTACAAGATG GAGATTGATG ATCTCTCCAG CAACATGGAG 
GCTGTTGCCA AGGCGAAGGG CAATTTGGAG AAAATGTGCA GAACTCTTGA GGACCAGCTG 
AGTGAGTTGA AGGCCAAAAA TGACGAGAAT GTTCGCCAAC TGAACGACAT CAATGCACAG 
AGGGCCAGAC TTCAGACAGA GAATGGTGAG TTTTCTCGCC AGCTTGAGGA GAAGGAAGCT 
CTTGTTTCCC AGCTGACCAG AGGCAAGCAG GCCTTCACCC AGCAGATTGA GGAGCTCAAG 
AGGCACATTG AGGAAGAAGT GAAGGCAAAG AATGCCCTGG CCCATGGTGT TCAGTCAGCT 
CGCCATGACT GTGATCTGCT CAGAGAGCAG TTTGAGGAGG AGCAGGAGGC CAAGGCTGAG 
CTGCAGAGAG GAATGTCCAA GGCCAACAGT GAGGTGGCTC AGTGGAGAAG CAAATATGAG 
ACTGATGCTA TCCAGCGCAC TGAGGAGCTG GAGGAGGCCA AGAAAAAGCT TGCCCAGCGC 
CTGCAGGAGG CTGAGGAATC CATTGAGGCT GTGAACTCCA AGTGTGCCTC TTTGGAGAAG 
ACCAAGCAGA GGCTGCAGGG TGAGGTGGAG GACCTCATGA TTGATGTGGA GAGAGCTAAC 
GCTCTGGCTG CCAACCTTGA CAAGAAGCAG AGGAACTTTG ATAAGGTCCT TGCAGAATGG 
AAGCAGAAGT ATGAGGAGGG CCAGGCAGAA CTGGAGGGAG CACAGAAGGA GGCTCGTTCT 
CTCAGCACCG AGTTGTTCAA GATGAAGAAC TCCTATGAGG AGGCTCTGGA TCACCTAGAG 
ACCATGAAGA GGGAGAACAA GAACCTGCAA CAGGAGATCT CAGATCTGAC TGAGCAGATC 
GGTGAGACTG GAAAGAGCAT CCATGAGCTG GAAAAAGCCA AGAAGACTGT TGAGACTGAG 
AAATCTGAAA TCCAGTCTGC ACTGGAGGAA GCTGAGGGTA CTCTGGAGCA TGAGGAGGCC 
AAGATTCTCC GTGTTCAGCT TGAGCTGAAC CAGATCAAAG GTGAGGTCGA CAGGAAGCTG 
GCAGAGAAGG ATGAGGAGAT GGAACAGATC AAGAGGAACA GCCAGAGGGT GATAGACTCC 
ATGCAGAGCA ATCTTGATGC TGAGGTCAGG AGCAGGAATG ATGCCCTGAG AGTCAAGAAG 
AAGATGGAGG GAGACCTGAA TGAGATGGAG ATTCAGCTGA GCCATGCCAA CAGGCAGGCT 
TCTGAGGCCC AGAAACAACT GAGGAACGTC CAGGGACAGC TCAAGGATGC TCAACTGCAC 
CTTGATGATG CCGTCAGAGG ACAGGAAGAC ATGAAGGAGC AGGTTGCCAT GGTGGAGCGC 
AGGAATGGTC TGATGATGGC TGAGATTGAA GAGCTGAGAG CTGCTCTGGA GCAGACCGAG 
AGAGGACGCA AAGTGGCTGA GCAGGAGTTG GTTGATGCTA GTGAGCGTGT CGGCCTGCTT 
CACTCCCAGA ACACCAGCCT TCTGAACACC AAGAAGAAGC TGGAGTCTGA CCTCGTCCAG 
GTCCAGGGTG AAGTGGATGA TGCTGTTCAG GAAGCAAGAA ATGCTGAGGA TAAAGCCAAA 
AAGGCTATCA CTGATGCTGC CATGATGGCT GAGGAGCTGA AGAAGGAGCA GGACACCAGC 
GCTCACCTGG AGAGGATGAA GAAGAACCTG GAGGTCACAG TCAAGGACCT GCAGCACCGT 
CTGGATGAGG CTGAGAACCT GGCCATGAAG GGTGGCAAGA AGCAGCTTCA GAAACTGGAA 
TCCAGGGTGC GTGAGCTTGA AACTGAAGTT GAAGGTGAGC AGAGACGTGG AGCTGATGCT 
GTCAAAGGAG TGCGCAAATA TGAGAGGAGA GTGAAGGAGC TGACCTACCA GACTGAGGAG 
GACAAGAAGA ACATGACCAG ACTTCAGGAT CTGGTGGATA AGCTGCAGCT CAAAGTCAAA 
GCTTACAAGA GACAGTCTGA GGAGGCTGAG GAACAGGCCA ACACGCACAT GTCCAGGCTC 
AGAAAGGTTC AGCATGAGCT GGAGGAAGCT CAGGAGCGTG CTGACATTGC TGAGTCCCAG 
GTCAACAAGC TGAGAGCCAA GAGCCGTGAT GCTGGAAAGA GTGAGTCTGC TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023144371.1
CDS	1..5817
Translation	MSTDAEMEQYGPAAIYLRKPERERIEAQTAPFDAKTAFFVPDNDEMYLKGKLVKKEGGKATVETDGGKSITVKEDEIHPRNPPKFDKMEDMAMMTHLNEPSVLYNLKERYASWMIYTYSGLFCVVVNPYKWLPVYDATCVAAYRGKKRIEAPPHIFSISDNAYQFMLTDRENQSVLITGESGAGKTVNTKRVIQYFATIASLGAKKAEATAGKMQGSLEDQIVAANPLLEAYGNAKTVRNDNSSRFGKFIRIHFGSSGKLASADIETYLLEKSRVTFQLSAERSYHIFYQLMTGHKPELLEALLITTNPYDYPMISQGEITVKSINDVEEFIATDTAIDILGFNAEEKINIYKLTGAVMHHGNMKFKQKQREEQAEPDGTEVADKIAYLFGLNSADMLKALCYPRVKVGNEMVTKGQTVPQVNNAVSALCKSVYEKMFLWMVIRINEMLDTKQPRQFFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHTMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKASDTTFKNKLHDQHLGKTKAFEKPKPGKGKAEAHFSLVHYAGTVDYNIVGWLDKNKDPLNESVVQLYQKSANKLLVYLYASHASAEEASGGGGKKGGKKKGGSFQTVSALFRENLGKLMTNLRSTHPHFVRCLIPNETKTPGLMENFLVIHQLRCNGVLEGIRICRKGFPSRILYGDFKQRYKVLNASVIPEGQFIDNKKASEKLLGSIDVDHTQYKFGHTKVFFKAGLLGTLEEMRDEKLASLVTMTQALCRGYVMRKEFVKMMERRESIYSIQYNIRSFMNVKNWPWLKLYFKIKPLLKSAETEKELQQMKENYDKMQSDLAAALAKKKELEEKMVSLLQEKNDLQLQVAAEVENLSDAEERCEGLIKSKIQLEAKLKETTERLEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMATQDESIAKLTKEKKALQESHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDLENDKQQSEEKIKKKDFETSQLLSKIEDEQSLGAQLQKKIKELQARIEELEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKLRRDLEESTLQHESTAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSEYKMEIDDLSSNMEAVAKAKGNLEKMCRTLEDQLSELKAKNDENVRQLNDINAQRARLQTENGEFSRQLEEKEALVSQLTRGKQAFTQQIEELKRHIEEEVKAKNALAHGVQSARHDCDLLREQFEEEQEAKAELQRGMSKANSEVAQWRSKYETDAIQRTEELEEAKKKLAQRLQEAEESIEAVNSKCASLEKTKQRLQGEVEDLMIDVERANALAANLDKKQRNFDKVLAEWKQKYEEGQAELEGAQKEARSLSTELFKMKNSYEEALDHLETMKRENKNLQQEISDLTEQIGETGKSIHELEKAKKTVETEKSEIQSALEEAEGTLEHEEAKILRVQLELNQIKGEVDRKLAEKDEEMEQIKRNSQRVIDSMQSNLDAEVRSRNDALRVKKKMEGDLNEMEIQLSHANRQASEAQKQLRNVQGQLKDAQLHLDDAVRGQEDMKEQVAMVERRNGLMMAEIEELRAALEQTERGRKVAEQELVDASERVGLLHSQNTSLLNTKKKLESDLVQVQGEVDDAVQEARNAEDKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEVTVKDLQHRLDEAENLAMKGGKKQLQKLESRVRELETEVEGEQRRGADAVKGVRKYERRVKELTYQTEEDKKNMTRLQDLVDKLQLKVKAYKRQSEEAEEQANTHMSRLRKVQHELEEAQERADIAESQVNKLRAKSRDAGKSESAE

Target ORF information:

RefSeq Version	XM_023288603.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris myosin heavy chain, fast skeletal muscle-like (LOC111580733), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023288603.1

ORF Insert Sequence:

ATGAGCACAG ACGCGGAGAT GGAGCAGTAT GGCCCGGCGG CCATTTACCT CCGGAAGCCA 
GAAAGGGAGA GGATTGAGGC CCAGACTGCT CCATTTGATG CCAAAACAGC CTTCTTTGTG 
CCCGATAATG ATGAGATGTA TCTCAAGGGT AAACTAGTCA AAAAGGAGGG TGGCAAAGCC 
ACAGTTGAGA CAGATGGGGG AAAGAGTATC ACTGTAAAAG AGGATGAGAT CCATCCCAGG 
AATCCTCCAA AGTTCGATAA GATGGAGGAC ATGGCCATGA TGACCCACCT CAACGAGCCA 
TCTGTGTTGT ATAACCTCAA AGAGCGTTAC GCCTCATGGA TGATCTATAC CTACTCTGGG 
TTGTTCTGCG TTGTCGTAAA TCCCTACAAA TGGCTTCCCG TGTATGACGC TACATGTGTA 
GCAGCATACA GAGGCAAAAA GAGGATTGAG GCTCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTCATGCT CACTGATCGT GAGAACCAGT CTGTCCTGAT CACCGGAGAA 
TCTGGTGCTG GAAAGACTGT CAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
TCTCTGGGAG CTAAGAAGGC TGAGGCAACA GCTGGCAAGA TGCAGGGCTC TCTTGAAGAC 
CAGATTGTTG CAGCCAACCC TCTGCTGGAG GCTTATGGTA ATGCCAAGAC TGTGAGGAAT 
GACAACTCCT CTCGTTTTGG TAAATTCATC AGGATCCACT TCGGCTCCTC TGGCAAGCTG 
GCTTCAGCTG ATATTGAAAC ATATCTGCTG GAGAAGTCTC GTGTCACCTT CCAGTTGTCT 
GCTGAGAGGA GCTACCATAT CTTCTATCAG CTGATGACTG GACACAAGCC TGAGCTCCTG 
GAGGCTCTTC TGATCACCAC TAACCCCTAT GACTATCCAA TGATCAGTCA GGGTGAAATC 
ACTGTCAAGA GCATCAATGA TGTGGAGGAG TTCATTGCAA CAGATACTGC TATTGACATC 
TTGGGTTTCA ACGCTGAAGA GAAAATCAAC ATCTACAAGC TGACCGGTGC TGTGATGCAT 
CATGGCAACA TGAAATTCAA GCAAAAGCAG AGAGAGGAGC AGGCTGAGCC TGATGGCACT 
GAGGTGGCTG ATAAAATCGC CTATCTCTTC GGCCTGAACT CAGCTGATAT GCTGAAAGCT 
CTGTGCTACC CAAGAGTCAA AGTCGGCAAT GAGATGGTCA CCAAAGGTCA GACTGTCCCA 
CAGGTCAACA ATGCTGTCAG TGCTCTGTGC AAGTCTGTCT ATGAGAAAAT GTTCTTGTGG 
ATGGTCATCC GTATCAATGA GATGCTGGAC ACAAAGCAGC CAAGACAGTT CTTCATTGGT 
GTGTTGGACA TCGCTGGATT TGAGATCTTT GATTTCAACA GCTTGGAGCA ACTCTGCATT 
AACTTCACCA ATGAGAAACT GCAACAGTTC TTCAACCACA CCATGTTTGT CCTGGAGCAA 
GAGGAGTACA AGAAAGAAGG TATTGAATGG GAGTTCATTG ACTTCGGTAT GGACTTGGCT 
GCCTGCATTG AGCTTATCGA GAAGCCAATG GGCATCTTCT CCATCCTTGA AGAGGAGTGC 
ATGTTCCCCA AGGCCTCTGA TACAACTTTC AAGAACAAGC TGCATGATCA GCATCTTGGC 
AAGACCAAGG CCTTTGAGAA GCCAAAGCCT GGAAAGGGCA AAGCTGAGGC TCACTTCTCC 
CTGGTTCACT ATGCTGGTAC AGTGGACTAC AATATTGTTG GCTGGCTGGA CAAGAACAAG 
GACCCACTGA ATGAATCTGT TGTTCAGCTC TACCAGAAGT CTGCAAACAA ACTGCTGGTC 
TACCTTTATG CATCCCATGC CTCAGCTGAG GAGGCTTCTG GTGGTGGTGG CAAGAAGGGT 
GGCAAGAAGA AGGGTGGCTC CTTCCAGACT GTGTCCGCTC TTTTCAGAGA GAACTTGGGC 
AAACTGATGA CCAACTTGAG GAGCACCCAT CCTCACTTTG TCCGTTGCTT GATTCCCAAT 
GAGACAAAGA CCCCAGGTCT TATGGAGAAC TTCTTGGTCA TCCACCAGCT GAGGTGTAAT 
GGTGTGCTGG AGGGCATCAG AATCTGTAGA AAGGGCTTCC CCAGCAGAAT TCTCTATGGT 
GACTTCAAGC AGAGATACAA AGTATTGAAT GCCAGTGTCA TCCCTGAGGG ACAGTTCATT 
GACAACAAGA AAGCTTCAGA GAAGCTGTTG GGCTCCATTG ATGTGGACCA CACTCAGTAC 
AAGTTTGGAC ACACTAAGGT GTTCTTCAAA GCTGGTCTGC TGGGTACCCT TGAGGAGATG 
AGAGATGAGA AACTGGCTTC ACTGGTGACC ATGACTCAAG CTCTCTGCAG AGGATATGTC 
ATGAGGAAGG AGTTTGTAAA GATGATGGAG AGGAGAGAAT CTATCTACTC CATCCAGTAC 
AATATCCGTT CATTCATGAA TGTGAAGAAC TGGCCATGGC TGAAGCTGTA CTTCAAGATC 
AAACCTCTTC TGAAGAGTGC TGAGACTGAG AAGGAGTTGC AGCAGATGAA GGAGAACTAT 
GATAAGATGC AGTCAGACCT GGCTGCTGCT TTGGCCAAGA AGAAGGAGCT GGAGGAGAAG 
ATGGTTTCCC TGCTGCAGGA GAAGAATGAC CTGCAACTCC AAGTGGCTGC AGAAGTTGAG 
AATCTCTCGG ATGCTGAGGA AAGGTGTGAG GGGCTCATTA AGAGCAAGAT CCAGCTCGAG 
GCCAAACTCA AAGAGACAAC TGAGAGACTG GAGGATGAAG AGGAAATCAA TGCTGAGCTG 
ACTGCCAAGA AGAGGAAGTT GGAGGATGAA TGCTCTGAGC TGAAGAAGGA CATTGATGAC 
TTGGAGCTCA CCTTGGCTAA AGTGGAGAAG GAGAAACATG CCACAGAAAA CAAGGTGAAA 
AACCTGACAG AGGAAATGGC CACTCAAGAT GAGTCTATTG CCAAGCTGAC CAAGGAGAAG 
AAAGCCCTCC AAGAGAGCCA TCAGCAGACA CTGGATGACC TCCAGGCAGA GGAAGACAAA 
GTCAACACTC TGACCAAGGC CAAGACAAAG CTGGAACAGC AAGTGGACGA TCTTGAGGGC 
TCACTGGAGC AGGAGAAGAA GCTCCGCATG GACCTTGAGA GAGCCAAGAG GAAGCTTGAG 
GGAGATCTGA AACTGGCCCA GGAATCCATA ATGGATCTGG AGAATGACAA GCAGCAGTCT 
GAGGAGAAAA TCAAGAAGAA GGACTTTGAG ACCAGTCAGC TCCTCAGCAA GATTGAGGAT 
GAACAGTCCC TTGGTGCTCA GCTTCAGAAG AAGATCAAGG AACTCCAGGC TCGCATTGAG 
GAGCTGGAGG AGGAGATTGA GGCTGAGAGG GCTGCTCGAG CTAAGGTAGA GAAGCAGAGA 
GCTGATCTCT CCAGGGAGCT TGAGGAGATC AGTGAGAGGC TTGAGGAGGC TGGTGGAGCA 
ACAGCTGCTC AGATTGAGAT GAACAAGAAG CGTGAGGCTG AGTTCCAGAA GTTGCGTCGT 
GACCTTGAAG AGTCAACCCT GCAGCACGAA TCTACTGCAG CAGCTCTCCG CAAGAAGCAG 
GCTGACAGCG TTGCAGAGCT GGGAGAGCAG ATCGACAACC TTCAGCGTGT CAAGCAGAAG 
CTGGAGAAGG AGAAGAGCGA GTACAAGATG GAGATTGATG ATCTCTCCAG CAACATGGAG 
GCTGTTGCCA AGGCGAAGGG CAATTTGGAG AAAATGTGCA GAACTCTTGA GGACCAGCTG 
AGTGAGTTGA AGGCCAAAAA TGACGAGAAT GTTCGCCAAC TGAACGACAT CAATGCACAG 
AGGGCCAGAC TTCAGACAGA GAATGGTGAG TTTTCTCGCC AGCTTGAGGA GAAGGAAGCT 
CTTGTTTCCC AGCTGACCAG AGGCAAGCAG GCCTTCACCC AGCAGATTGA GGAGCTCAAG 
AGGCACATTG AGGAAGAAGT GAAGGCAAAG AATGCCCTGG CCCATGGTGT TCAGTCAGCT 
CGCCATGACT GTGATCTGCT CAGAGAGCAG TTTGAGGAGG AGCAGGAGGC CAAGGCTGAG 
CTGCAGAGAG GAATGTCCAA GGCCAACAGT GAGGTGGCTC AGTGGAGAAG CAAATATGAG 
ACTGATGCTA TCCAGCGCAC TGAGGAGCTG GAGGAGGCCA AGAAAAAGCT TGCCCAGCGC 
CTGCAGGAGG CTGAGGAATC CATTGAGGCT GTGAACTCCA AGTGTGCCTC TTTGGAGAAG 
ACCAAGCAGA GGCTGCAGGG TGAGGTGGAG GACCTCATGA TTGATGTGGA GAGAGCTAAC 
GCTCTGGCTG CCAACCTTGA CAAGAAGCAG AGGAACTTTG ATAAGGTCCT TGCAGAATGG 
AAGCAGAAGT ATGAGGAGGG CCAGGCAGAA CTGGAGGGAG CACAGAAGGA GGCTCGTTCT 
CTCAGCACCG AGTTGTTCAA GATGAAGAAC TCCTATGAGG AGGCTCTGGA TCACCTAGAG 
ACCATGAAGA GGGAGAACAA GAACCTGCAA CAGGAGATCT CAGATCTGAC TGAGCAGATC 
GGTGAGACTG GAAAGAGCAT CCATGAGCTG GAAAAAGCCA AGAAGACTGT TGAGACTGAG 
AAATCTGAAA TCCAGTCTGC ACTGGAGGAA GCTGAGGGTA CTCTGGAGCA TGAGGAGGCC 
AAGATTCTCC GTGTTCAGCT TGAGCTGAAC CAGATCAAAG GTGAGGTCGA CAGGAAGCTG 
GCAGAGAAGG ATGAGGAGAT GGAACAGATC AAGAGGAACA GCCAGAGGGT GATAGACTCC 
ATGCAGAGCA ATCTTGATGC TGAGGTCAGG AGCAGGAATG ATGCCCTGAG AGTCAAGAAG 
AAGATGGAGG GAGACCTGAA TGAGATGGAG ATTCAGCTGA GCCATGCCAA CAGGCAGGCT 
TCTGAGGCCC AGAAACAACT GAGGAACGTC CAGGGACAGC TCAAGGATGC TCAACTGCAC 
CTTGATGATG CCGTCAGAGG ACAGGAAGAC ATGAAGGAGC AGGTTGCCAT GGTGGAGCGC 
AGGAATGGTC TGATGATGGC TGAGATTGAA GAGCTGAGAG CTGCTCTGGA GCAGACCGAG 
AGAGGACGCA AAGTGGCTGA GCAGGAGTTG GTTGATGCTA GTGAGCGTGT CGGCCTGCTT 
CACTCCCAGA ACACCAGCCT TCTGAACACC AAGAAGAAGC TGGAGTCTGA CCTCGTCCAG 
GTCCAGGGTG AAGTGGATGA TGCTGTTCAG GAAGCAAGAA ATGCTGAGGA TAAAGCCAAA 
AAGGCTATCA CTGATGCTGC CATGATGGCT GAGGAGCTGA AGAAGGAGCA GGACACCAGC 
GCTCACCTGG AGAGGATGAA GAAGAACCTG GAGGTCACAG TCAAGGACCT GCAGCACCGT 
CTGGATGAGG CTGAGAACCT GGCCATGAAG GGTGGCAAGA AGCAGCTTCA GAAACTGGAA 
TCCAGGGTGC GTGAGCTTGA AACTGAAGTT GAAGGTGAGC AGAGACGTGG AGCTGATGCT 
GTCAAAGGAG TGCGCAAATA TGAGAGGAGA GTGAAGGAGC TGACCTACCA GACTGAGGAG 
GACAAGAAGA ACATGACCAG ACTTCAGGAT CTGGTGGATA AGCTGCAGCT CAAAGTCAAA 
GCTTACAAGA GACAGTCTGA GGAGGCTGAG GAACAGGCCA ACACGCACAT GTCCAGGCTC 
AGAAAGGTTC AGCATGAGCT GGAGGAAGCT CAGGAGCGTG CTGACATTGC TGAGTCCCAG 
GTCAACAAGC TGAGAGCCAA GAGCCGTGAT GCTGGAAAGA GTGAGTCTGC TGAATAA