vps53 cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	vps53
Entrez Gene ID	111580674
Full Name	VPS53, GARP complex subunit
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023288520.1	XP_023144288.1	vacuolar protein sorting-associated protein 53 homolog

Pathways from BioSystems
Gene Ontology

The following vps53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vps53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm92264	XM_023288520.1 Latest version!	Amphiprion ocellaris VPS53, GARP complex subunit (vps53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vps53 ( XM_023288520.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023144288.1 Amphiprion ocellaris VPS53, GARP complex subunit (vps53), mRNA.

Price & Availability↑

CloneID	OAm92264
Clone ID Related Accession (Same CDS sequence)	XM_023288520.1
Accession Version	XM_023288520.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	vacuolar protein sorting-associated protein 53 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019373440.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGAGG AAGAAGAATT TGAATTTGCT GAGGACCTGG AGGCTATTTT GCACCTAACA 
CCAGAGGTGC AACTAGCCAT TGAACAGGTT TTCCCCAGCC AGGATCCACT GGACAGAGCA 
GACTTCAATG CTGTGGAGTA CATCAACACA CTTTTCCCCA CCGAGCAGTC TTTGGCTAAC 
ATCGATGATG TGGTGAATAA GATACGTCTG AAGATTCGAC GTCTGGATGA CAACATCAGG 
ACGGTGGTGA GAGGCCAAAC CAATGTGGGT CAGGATGGCC GACAGGCTTT GGAGGAGGCT 
CAGATAGCCA TCCAGCAGCT CTTTGGCAAA ATCAAAGACA TTAAGGACAA GGCAGAGAAG 
TCTGAACAAA TGGTCAAGGA GATTACGAGG GACATAAAGC AGTTGGACCA TGCCAAACGC 
CACCTCACAA CTTCCATCAC CACCCTGAAC CACCTACACA TGCTAGCAGG AGGCGTTGAC 
TCTTTAGAAG CCATGACCAG AAAGAGGCAG TATGGTGAGG TGGCCAACCT GCTGCAGGGA 
GTGGTGAATG TACTTGAACA TTTCCACAAG TACATGGGCA TACCCCAGAT TAGACAGCTT 
TCTGAGAGAG TAAAGGCAGC ACAGAGCGAG CTGGGGACTC AGATCCTGGC AGATTTTGAA 
GAAGCATTCC CCTCTCAGGG AACAAAGAGA CCAGGTGGAC CCAGTAATGT TCTGAGAGAT 
GCCTGTCTGG TGGCAAATGT TCTCGACCCG CGCATCAAAC AGGAGATCAT CAAAAAGTTC 
ATCAGGCAGC ATCTCTCGGA GTATCTGGTC CTCTTCCAGG AAAATCAAGA TGTTGCTTGG 
CTGGACAAGA TCGATCGCCG CTATGCCTGG ATCAAACGGC AACTGTTAGA CTATGAAGAA 
AAATACGGTC GCATGTTTCC TGAGGAGTGG TGCATGACAG AGCGTATTGC TGTGGAGTTC 
TGCCACATCA CGAGGGTGGA GCTGGCTAAA GTGATGCGAA CACGTGCTAA AGAGATTGAG 
GTGAAACTGC TTCTGTTTGC CATTCAGAGG ACCACAAACT TCGAGGGTTT ACTGGCCAAA 
CGCTTCACGG GATGCACCTT GACGGACACA CCTGGACAAA AAAGGCCAGA AAGCCCATTA 
GACCCAACTA ACCCCTTTCT GGAGGATGAG CCAGGAGAGG ATGTGGGCAC AGACAAAGAT 
GAGGATCTTG CTAAACCCAG GAAACCCAAA GCTCCGGACA ACCCTTTCCA TGGCATTGTC 
TCAAAGTGCT TTGAACCTCA TCTATATGTC TACATAGAAT CCCAAGACAA GAACCTGGGT 
GAACTGATTG ACCGATTTGT GGCTGATTTC CGAGCACAAG GACCCCCCAA GGCAGGCACC 
GGGGAGGGTG GAGCAGTACT GCCCAGCTGT GCTGATCTCT TTGTTTATTA CAAGAAGTGT 
ATGGTGCAGT GCTCCCAACT GAGCACAGGA GAACCAATGA TCGCCCTCAC CACCATCTTC 
CAGAAATTCC TCAGAGAGTA TGCTTGGAAA ATTCTCTCTG GCAACCTGCC CAAATCCAGC 
AGTAACAGTG GGGGTCTCAC CATCAGCAGT CTGCTTAAGG AAAAAGAAGG TTCTGAAGCA 
GCAAAGTTCA CTGTAGATGA GCTCTGCCTG ATCTGTAGCA TTCTTAGTAC TGCAGAGTAC 
TGTCTGGCTA CCACACAACA GTTGGAAGAG AAGCTAAAGG AGAAAGTAGA TAAAGTCTTG 
GTGGAAAGAA TTAATTTGAC TGGAGAGATG GATACATTCA GCACTGTGAT CTCAAACAGC 
ATCCAGCTGC TTGTTCAAGA TCTCGATGCA GCCTGCGATC CTGCTCTAAC GGCTATGAGC 
AAGATGCCGT GGCAGAGTGT GGAACATGTG GGTGACCAGA GTCCTTATGT GACATCAATT 
ATCATGCACA TTAAACAAAA TGTGCCAATC ATCAGAGACA ATCTTGCCTC GACACGCAAA 
TACTTCACGC AGTTCTGCAT CAAGTTCACA AACTCTTTCA TTCCCAAATT CATCAACCAC 
CTGTTTAGAT GTAAGCCTAT CAGCATGGTG GGAGCTGAAC AGCTCCTCCT TGACACACAC 
TCCCTGAAGA CAGTCCTGTT GGATCTGCCG TCCATAGGTT CCCAGGTGCT CCGCAAAGCA 
CCTGCCAGCT ACACTAAGAT AGTGGTGAAA GGCATGACGC GTGCAGAGAT GATACTTAAG 
GTGGTGATGG CCCCACATGA ACCACCAGTG GTATTTGTTG ATAACTACAT CAAGCTCCTG 
GCTGATGGCA ATCCTGAGAC CTTCCAGAAG ATCCTGGATA TGAAGGGTCT GAAGCGCAGT 
GAACAGAGCA GCATGCTGGA GCTCTTCAGG CAGAGGTTAC CCACTCCACC CTCTGGGGCC 
GACGGTGGAC CCTCTCTGTC CTTCAGTGCC CCCACCCCTG AGCAGGAGTC CTCTCGTATC 
CGCAAACTAG AGAAACTAAT CAAGAAGAGA CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023144288.1
CDS	44..2539
Translation	MVEEEEFEFAEDLEAILHLTPEVQLAIEQVFPSQDPLDRADFNAVEYINTLFPTEQSLANIDDVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQALEEAQIAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRKRQYGEVANLLQGVVNVLEHFHKYMGIPQIRQLSERVKAAQSELGTQILADFEEAFPSQGTKRPGGPSNVLRDACLVANVLDPRIKQEIIKKFIRQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLLDYEEKYGRMFPEEWCMTERIAVEFCHITRVELAKVMRTRAKEIEVKLLLFAIQRTTNFEGLLAKRFTGCTLTDTPGQKRPESPLDPTNPFLEDEPGEDVGTDKDEDLAKPRKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFRAQGPPKAGTGEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKFLREYAWKILSGNLPKSSSNSGGLTISSLLKEKEGSEAAKFTVDELCLICSILSTAEYCLATTQQLEEKLKEKVDKVLVERINLTGEMDTFSTVISNSIQLLVQDLDAACDPALTAMSKMPWQSVEHVGDQSPYVTSIIMHIKQNVPIIRDNLASTRKYFTQFCIKFTNSFIPKFINHLFRCKPISMVGAEQLLLDTHSLKTVLLDLPSIGSQVLRKAPASYTKIVVKGMTRAEMILKVVMAPHEPPVVFVDNYIKLLADGNPETFQKILDMKGLKRSEQSSMLELFRQRLPTPPSGADGGPSLSFSAPTPEQESSRIRKLEKLIKKRL

Target ORF information:

RefSeq Version	XM_023288520.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris VPS53, GARP complex subunit (vps53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023288520.1

ORF Insert Sequence:

ATGGTGGAGG AAGAAGAATT TGAATTTGCT GAGGACCTGG AGGCTATTTT GCACCTAACA 
CCAGAGGTGC AACTAGCCAT TGAACAGGTT TTCCCCAGCC AGGATCCACT GGACAGAGCA 
GACTTCAATG CTGTGGAGTA CATCAACACA CTTTTCCCCA CCGAGCAGTC TTTGGCTAAC 
ATCGATGATG TGGTGAATAA GATACGTCTG AAGATTCGAC GTCTGGATGA CAACATCAGG 
ACGGTGGTGA GAGGCCAAAC CAATGTGGGT CAGGATGGCC GACAGGCTTT GGAGGAGGCT 
CAGATAGCCA TCCAGCAGCT CTTTGGCAAA ATCAAAGACA TTAAGGACAA GGCAGAGAAG 
TCTGAACAAA TGGTCAAGGA GATTACGAGG GACATAAAGC AGTTGGACCA TGCCAAACGC 
CACCTCACAA CTTCCATCAC CACCCTGAAC CACCTACACA TGCTAGCAGG AGGCGTTGAC 
TCTTTAGAAG CCATGACCAG AAAGAGGCAG TATGGTGAGG TGGCCAACCT GCTGCAGGGA 
GTGGTGAATG TACTTGAACA TTTCCACAAG TACATGGGCA TACCCCAGAT TAGACAGCTT 
TCTGAGAGAG TAAAGGCAGC ACAGAGCGAG CTGGGGACTC AGATCCTGGC AGATTTTGAA 
GAAGCATTCC CCTCTCAGGG AACAAAGAGA CCAGGTGGAC CCAGTAATGT TCTGAGAGAT 
GCCTGTCTGG TGGCAAATGT TCTCGACCCG CGCATCAAAC AGGAGATCAT CAAAAAGTTC 
ATCAGGCAGC ATCTCTCGGA GTATCTGGTC CTCTTCCAGG AAAATCAAGA TGTTGCTTGG 
CTGGACAAGA TCGATCGCCG CTATGCCTGG ATCAAACGGC AACTGTTAGA CTATGAAGAA 
AAATACGGTC GCATGTTTCC TGAGGAGTGG TGCATGACAG AGCGTATTGC TGTGGAGTTC 
TGCCACATCA CGAGGGTGGA GCTGGCTAAA GTGATGCGAA CACGTGCTAA AGAGATTGAG 
GTGAAACTGC TTCTGTTTGC CATTCAGAGG ACCACAAACT TCGAGGGTTT ACTGGCCAAA 
CGCTTCACGG GATGCACCTT GACGGACACA CCTGGACAAA AAAGGCCAGA AAGCCCATTA 
GACCCAACTA ACCCCTTTCT GGAGGATGAG CCAGGAGAGG ATGTGGGCAC AGACAAAGAT 
GAGGATCTTG CTAAACCCAG GAAACCCAAA GCTCCGGACA ACCCTTTCCA TGGCATTGTC 
TCAAAGTGCT TTGAACCTCA TCTATATGTC TACATAGAAT CCCAAGACAA GAACCTGGGT 
GAACTGATTG ACCGATTTGT GGCTGATTTC CGAGCACAAG GACCCCCCAA GGCAGGCACC 
GGGGAGGGTG GAGCAGTACT GCCCAGCTGT GCTGATCTCT TTGTTTATTA CAAGAAGTGT 
ATGGTGCAGT GCTCCCAACT GAGCACAGGA GAACCAATGA TCGCCCTCAC CACCATCTTC 
CAGAAATTCC TCAGAGAGTA TGCTTGGAAA ATTCTCTCTG GCAACCTGCC CAAATCCAGC 
AGTAACAGTG GGGGTCTCAC CATCAGCAGT CTGCTTAAGG AAAAAGAAGG TTCTGAAGCA 
GCAAAGTTCA CTGTAGATGA GCTCTGCCTG ATCTGTAGCA TTCTTAGTAC TGCAGAGTAC 
TGTCTGGCTA CCACACAACA GTTGGAAGAG AAGCTAAAGG AGAAAGTAGA TAAAGTCTTG 
GTGGAAAGAA TTAATTTGAC TGGAGAGATG GATACATTCA GCACTGTGAT CTCAAACAGC 
ATCCAGCTGC TTGTTCAAGA TCTCGATGCA GCCTGCGATC CTGCTCTAAC GGCTATGAGC 
AAGATGCCGT GGCAGAGTGT GGAACATGTG GGTGACCAGA GTCCTTATGT GACATCAATT 
ATCATGCACA TTAAACAAAA TGTGCCAATC ATCAGAGACA ATCTTGCCTC GACACGCAAA 
TACTTCACGC AGTTCTGCAT CAAGTTCACA AACTCTTTCA TTCCCAAATT CATCAACCAC 
CTGTTTAGAT GTAAGCCTAT CAGCATGGTG GGAGCTGAAC AGCTCCTCCT TGACACACAC 
TCCCTGAAGA CAGTCCTGTT GGATCTGCCG TCCATAGGTT CCCAGGTGCT CCGCAAAGCA 
CCTGCCAGCT ACACTAAGAT AGTGGTGAAA GGCATGACGC GTGCAGAGAT GATACTTAAG 
GTGGTGATGG CCCCACATGA ACCACCAGTG GTATTTGTTG ATAACTACAT CAAGCTCCTG 
GCTGATGGCA ATCCTGAGAC CTTCCAGAAG ATCCTGGATA TGAAGGGTCT GAAGCGCAGT 
GAACAGAGCA GCATGCTGGA GCTCTTCAGG CAGAGGTTAC CCACTCCACC CTCTGGGGCC 
GACGGTGGAC CCTCTCTGTC CTTCAGTGCC CCCACCCCTG AGCAGGAGTC CTCTCGTATC 
CGCAAACTAG AGAAACTAAT CAAGAAGAGA CTGTGA