dagla cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dagla
Entrez Gene ID	111574610
Full Name	diacylglycerol lipase alpha
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023279289.1	XP_023135057.1	sn1-specific diacylglycerol lipase alpha isoform X2
XM_023279288.1	XP_023135056.1	sn1-specific diacylglycerol lipase alpha isoform X1

Pathways from BioSystems
Gene Ontology

The following dagla gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dagla cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm84992	XM_023279288.1 Latest version!	Amphiprion ocellaris diacylglycerol lipase alpha (dagla), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAm84993	XM_023279289.1 Latest version!	Amphiprion ocellaris diacylglycerol lipase alpha (dagla), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dagla ( XM_023279288.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023135056.1 Amphiprion ocellaris diacylglycerol lipase alpha (dagla), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAm84992
Clone ID Related Accession (Same CDS sequence)	XM_023279288.1
Accession Version	XM_023279288.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	sn1-specific diacylglycerol lipase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019372821.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAA TGGTGATGTT TCGGCGGCGC TGGTCAGTTG GCAGTGATGA CCTGGTGCTG 
CCCGCCCTCT TCCTTTTCTT ATTACACTGC ATCTGGTTGG TGGTTCTGTC CGTGGTTCTC 
TTTGGCCTTC CATATGGCTC AGACCAGTCC TGCTCTGTAA CACTGGTGGA CCATGGCCGA 
GGGTACCTGG GCATCCTGGT CAGCTGCCTT ATATGTGAGA GCGCCATCAT GTGGTTGAGT 
ATGAGGGGCA GCATTCTGTA CACACAGCCC AGGGAAGCTG TGCAATATGT CATCTATATA 
CGACTAGCTA TTCTGTTGGT TGAGCTAGTA TATGCTGTAG TAGGAATTGC CTGGCTTGTC 
CAGTATTACC AGCCGTGCTC TGATGTCACT GCCAAAAACT TGGCTTTGGG AATTGTTGCA 
TGCAACTGGC TAGTGATATT CAGCGTTTGC ATCACGCTCA TGTGCACCTT TGATCCTACG 
GGTCGGACTT TTGTCAAACT GAAAGCCACC CGTCGGCGTC AGCGTAACCT CACTACATAC 
ACACTCAGAC ACAGGCTAGA GGAAGGCCAA GCCAGTAGCT GGAGCAGGAG ACTAAAGTTC 
TTCATGTGCT GCACAAGAAC CCAAGATACA CAATCAGATG CGTATTCAGA AGTGGCCAGC 
CTTTTTGCAG AGTTCTTCAG AGACCTGGAT ATTGTGCCTA GTGATATTAT TGCTGGCCTG 
GTACTTCTCC GCCAGAGGCA AAGGTCTAAG AGATCTGCTA TCCTGGACCA GGCCAACAAT 
GACATTTTAG CCTTCTTATC AGGAATGCCT GTCACTCGAA ACACTAGATA TTTGGACCTG 
AAGAACTCGG CTGAGATGAA CATGTACAAA GATGTGTGTT ATTATATGCT CTTTGCCCTG 
GCTGCATATG GTTGGCCCAT GTACCTGATG AGGAAGCCTG CCTGTGGGCT GTGCCGCCTC 
GCCAGCTCTT GCCCCTGTAC ATCAGTGTCT GGCTCTCGGT TGTCTCAGTC TGTGACAGTG 
GAGGAGGACA ACTGTTGCGG CTGTAACGTC CTGGCCATAC GTAGACACTT CCTGGACAGG 
GATCTCAAGC AGGTTCACAT TGTCTACACA TCCTGCCACG ATGCTGTTTA TGAAACTCCA 
TTTTTTGTGG CAGTTGATCA CGCGAAAAAG AAAGTTGTCA TCAGTATCAG AGGAACCCTT 
TCACCAAAGG ATGCCTTGAC TGATCTGACA GGAGACTCTG AACGTTTGCC TGTGGAGGAG 
CAACATGGGA CTTGGCTCGG CCACAAGGGA ATGGTTTACT CAGCAGAATA CATTAAGAAG 
AAACTGGAGC AGGAAATGAT CCTGTCACAA GCGTTTGGAA GAGACTTGAA CAAGGGCACC 
ATGCACTATG GGCTGGTGAT AGTTGGGCAT TCTCTTGGTG CAGGCACTGC TACTATCCTC 
TCCTTCCTGC TGAGACCTCA GTACCCTACA CTTCACTGCT ACTCTTATTC TCCACCTGGT 
GGCCTTCTTA GTGAGGATGC CATGGAGTAC TCCAAAGAGT TCGTCACGTC CGTGGTATTA 
GGAAAAGACC TGGTACCAAG GCTGGGCCTG TCACAACTGG AGGGTTTCCG ACGCCACCTT 
CTGGAAGTCT TACAGAAAAG TAACAAGCCA AAGTGGAGGA TCATTGCAGG AGGCACTAAA 
TGCATTCCAA AGTCAGAACT TCCACTAGAG GATGATGATC CTCAGTCTCA GCCGGCAGCA 
CCCCCCAGCA GTCGCCTGTG GCTGCATCCC AGTGACCTCA GCATTGCCCT GTCAGCCTCC 
ACACCCCTCT TCCCTCCTGG CAGAATCATC CACGTGGTGC ACAACCATCC TCCAGAGACA 
TGCTGTGGCC AGGAAGAGCC GACCTATTCA GCTCTGTGGG GGGACAACAA GGCCTTCAAT 
GAGGTCATAA TATCACCCGC CATGCTTAAT GAGCACATGC CCCACATGGT GATGGAGGGC 
CTAAACAAGG TTCTGGAGAA CTATAACAAA GGGAAAACTG CTCTGCTGTC AGCAGCCAAG 
ATCATGGTGA GCCCCACTGA AGTGGACTTG AACCCAGAGA CCATGTTCGT GGATACATCA 
ACAACCTCTC AGCAGCCGAC GCCTACAACC CACCACCGCA GGAACAGTAG TGTTCGACAA 
AAACCATTGT CACGTTCCTG TGCCCAGTCT GAAATATCTC TGGACGGTTT CTCTGAGTGC 
CCTCCTCCCC CAGTGCCTGT AGTGCTGCGT GGAGCACGGG AGCGCCTGGC AGTGGAGCTG 
AGGGACCGCA AAGCACCGCT GGCCGTCATG GAGAGCCTCT CAGACGCCGA GTCTCTCTAC 
AGTCTGGATT CCCGACGCTC CTCAGCTGCG TTAAGGGGTT CGCCAATGCT GGGTAGCCTT 
CCATTCCCTT TGGATGCCCC GATCCCAGAG GAGAACCCGT CTCTCAGCTC TCGCACTGAA 
CTGCTGGCTG CGGATTGCCT CTGCCAGGAA ACACCAGAAC ACCTTGGTGA GGTTGGGCCC 
TTCTTTACCC CACTGGAATC CAGATCCACC CCAGACTACC CCATGCGCTT GTCTCCTGCC 
ACGCCTCGCT ATAGTGGACA CCATTCCCCA GCTCTTCTCA ACAAGAGTGC CGTGGCTCAG 
AGTTTCAAGC CGGAGCCTAA CACCACCCCA AAAATGCTTC CTGAGGGGGA TCACCAACAG 
ATGCCCGGTG TCTACAGTCC AGGGAGCACT CCTATTGCTC CCCTAAGCCC ACAGATTGGT 
TCTAGACCAA TGGATGGAGA AAAGGAAGAC TGGGAGCTGG ACACAGCAGA GAAACAGTTT 
GGTGCAGAGA CAACAGCTGC TACTCCTCCA CCACAGCTAT CCAATGGAAA CCCCAGCCAG 
GCAGTGCTGG AGTTCGCCCA GTACTTAGAC TCTCTATTCA GGCTGGACGG CAGCAATTCA 
CCGCCTCTGG AGCTGTCCGA CGGGGAGTCG GAGTCTGGCC GAGGCTCCTT TGGCCAGGGT 
GAATGCCTCG GAGATGGACA GCAGTTGGAC GACAAACAGC TTCTGGCCAG AGCAACACTG 
GAGCCAAATC TAGTCCCCAA GCCCCCACGC ACTTTTGCTG GATCTGCCGA CCCCTCTTCG 
GGCATATCTT TGTCACCTTC CTTCCCTCTC TCATCGTCTG AAGAGCTCAA TGACCTCTCC 
CCCAGTGAGG GTGCTCCGCC AGCCATACAC TCCCTACGAA CATCTTCTCC TTGCCACTGT 
CCTGAGGAGA ACACACTATC ATCTATGGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023135056.1
CDS	923..4195
Translation	MPGMVMFRRRWSVGSDDLVLPALFLFLLHCIWLVVLSVVLFGLPYGSDQSCSVTLVDHGRGYLGILVSCLICESAIMWLSMRGSILYTQPREAVQYVIYIRLAILLVELVYAVVGIAWLVQYYQPCSDVTAKNLALGIVACNWLVIFSVCITLMCTFDPTGRTFVKLKATRRRQRNLTTYTLRHRLEEGQASSWSRRLKFFMCCTRTQDTQSDAYSEVASLFAEFFRDLDIVPSDIIAGLVLLRQRQRSKRSAILDQANNDILAFLSGMPVTRNTRYLDLKNSAEMNMYKDVCYYMLFALAAYGWPMYLMRKPACGLCRLASSCPCTSVSGSRLSQSVTVEEDNCCGCNVLAIRRHFLDRDLKQVHIVYTSCHDAVYETPFFVAVDHAKKKVVISIRGTLSPKDALTDLTGDSERLPVEEQHGTWLGHKGMVYSAEYIKKKLEQEMILSQAFGRDLNKGTMHYGLVIVGHSLGAGTATILSFLLRPQYPTLHCYSYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRLGLSQLEGFRRHLLEVLQKSNKPKWRIIAGGTKCIPKSELPLEDDDPQSQPAAPPSSRLWLHPSDLSIALSASTPLFPPGRIIHVVHNHPPETCCGQEEPTYSALWGDNKAFNEVIISPAMLNEHMPHMVMEGLNKVLENYNKGKTALLSAAKIMVSPTEVDLNPETMFVDTSTTSQQPTPTTHHRRNSSVRQKPLSRSCAQSEISLDGFSECPPPPVPVVLRGARERLAVELRDRKAPLAVMESLSDAESLYSLDSRRSSAALRGSPMLGSLPFPLDAPIPEENPSLSSRTELLAADCLCQETPEHLGEVGPFFTPLESRSTPDYPMRLSPATPRYSGHHSPALLNKSAVAQSFKPEPNTTPKMLPEGDHQQMPGVYSPGSTPIAPLSPQIGSRPMDGEKEDWELDTAEKQFGAETTAATPPPQLSNGNPSQAVLEFAQYLDSLFRLDGSNSPPLELSDGESESGRGSFGQGECLGDGQQLDDKQLLARATLEPNLVPKPPRTFAGSADPSSGISLSPSFPLSSSEELNDLSPSEGAPPAIHSLRTSSPCHCPEENTLSSMV

Target ORF information:

RefSeq Version	XM_023279288.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris diacylglycerol lipase alpha (dagla), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023279288.1

ORF Insert Sequence:

ATGCCTGGAA TGGTGATGTT TCGGCGGCGC TGGTCAGTTG GCAGTGATGA CCTGGTGCTG 
CCCGCCCTCT TCCTTTTCTT ATTACACTGC ATCTGGTTGG TGGTTCTGTC CGTGGTTCTC 
TTTGGCCTTC CATATGGCTC AGACCAGTCC TGCTCTGTAA CACTGGTGGA CCATGGCCGA 
GGGTACCTGG GCATCCTGGT CAGCTGCCTT ATATGTGAGA GCGCCATCAT GTGGTTGAGT 
ATGAGGGGCA GCATTCTGTA CACACAGCCC AGGGAAGCTG TGCAATATGT CATCTATATA 
CGACTAGCTA TTCTGTTGGT TGAGCTAGTA TATGCTGTAG TAGGAATTGC CTGGCTTGTC 
CAGTATTACC AGCCGTGCTC TGATGTCACT GCCAAAAACT TGGCTTTGGG AATTGTTGCA 
TGCAACTGGC TAGTGATATT CAGCGTTTGC ATCACGCTCA TGTGCACCTT TGATCCTACG 
GGTCGGACTT TTGTCAAACT GAAAGCCACC CGTCGGCGTC AGCGTAACCT CACTACATAC 
ACACTCAGAC ACAGGCTAGA GGAAGGCCAA GCCAGTAGCT GGAGCAGGAG ACTAAAGTTC 
TTCATGTGCT GCACAAGAAC CCAAGATACA CAATCAGATG CGTATTCAGA AGTGGCCAGC 
CTTTTTGCAG AGTTCTTCAG AGACCTGGAT ATTGTGCCTA GTGATATTAT TGCTGGCCTG 
GTACTTCTCC GCCAGAGGCA AAGGTCTAAG AGATCTGCTA TCCTGGACCA GGCCAACAAT 
GACATTTTAG CCTTCTTATC AGGAATGCCT GTCACTCGAA ACACTAGATA TTTGGACCTG 
AAGAACTCGG CTGAGATGAA CATGTACAAA GATGTGTGTT ATTATATGCT CTTTGCCCTG 
GCTGCATATG GTTGGCCCAT GTACCTGATG AGGAAGCCTG CCTGTGGGCT GTGCCGCCTC 
GCCAGCTCTT GCCCCTGTAC ATCAGTGTCT GGCTCTCGGT TGTCTCAGTC TGTGACAGTG 
GAGGAGGACA ACTGTTGCGG CTGTAACGTC CTGGCCATAC GTAGACACTT CCTGGACAGG 
GATCTCAAGC AGGTTCACAT TGTCTACACA TCCTGCCACG ATGCTGTTTA TGAAACTCCA 
TTTTTTGTGG CAGTTGATCA CGCGAAAAAG AAAGTTGTCA TCAGTATCAG AGGAACCCTT 
TCACCAAAGG ATGCCTTGAC TGATCTGACA GGAGACTCTG AACGTTTGCC TGTGGAGGAG 
CAACATGGGA CTTGGCTCGG CCACAAGGGA ATGGTTTACT CAGCAGAATA CATTAAGAAG 
AAACTGGAGC AGGAAATGAT CCTGTCACAA GCGTTTGGAA GAGACTTGAA CAAGGGCACC 
ATGCACTATG GGCTGGTGAT AGTTGGGCAT TCTCTTGGTG CAGGCACTGC TACTATCCTC 
TCCTTCCTGC TGAGACCTCA GTACCCTACA CTTCACTGCT ACTCTTATTC TCCACCTGGT 
GGCCTTCTTA GTGAGGATGC CATGGAGTAC TCCAAAGAGT TCGTCACGTC CGTGGTATTA 
GGAAAAGACC TGGTACCAAG GCTGGGCCTG TCACAACTGG AGGGTTTCCG ACGCCACCTT 
CTGGAAGTCT TACAGAAAAG TAACAAGCCA AAGTGGAGGA TCATTGCAGG AGGCACTAAA 
TGCATTCCAA AGTCAGAACT TCCACTAGAG GATGATGATC CTCAGTCTCA GCCGGCAGCA 
CCCCCCAGCA GTCGCCTGTG GCTGCATCCC AGTGACCTCA GCATTGCCCT GTCAGCCTCC 
ACACCCCTCT TCCCTCCTGG CAGAATCATC CACGTGGTGC ACAACCATCC TCCAGAGACA 
TGCTGTGGCC AGGAAGAGCC GACCTATTCA GCTCTGTGGG GGGACAACAA GGCCTTCAAT 
GAGGTCATAA TATCACCCGC CATGCTTAAT GAGCACATGC CCCACATGGT GATGGAGGGC 
CTAAACAAGG TTCTGGAGAA CTATAACAAA GGGAAAACTG CTCTGCTGTC AGCAGCCAAG 
ATCATGGTGA GCCCCACTGA AGTGGACTTG AACCCAGAGA CCATGTTCGT GGATACATCA 
ACAACCTCTC AGCAGCCGAC GCCTACAACC CACCACCGCA GGAACAGTAG TGTTCGACAA 
AAACCATTGT CACGTTCCTG TGCCCAGTCT GAAATATCTC TGGACGGTTT CTCTGAGTGC 
CCTCCTCCCC CAGTGCCTGT AGTGCTGCGT GGAGCACGGG AGCGCCTGGC AGTGGAGCTG 
AGGGACCGCA AAGCACCGCT GGCCGTCATG GAGAGCCTCT CAGACGCCGA GTCTCTCTAC 
AGTCTGGATT CCCGACGCTC CTCAGCTGCG TTAAGGGGTT CGCCAATGCT GGGTAGCCTT 
CCATTCCCTT TGGATGCCCC GATCCCAGAG GAGAACCCGT CTCTCAGCTC TCGCACTGAA 
CTGCTGGCTG CGGATTGCCT CTGCCAGGAA ACACCAGAAC ACCTTGGTGA GGTTGGGCCC 
TTCTTTACCC CACTGGAATC CAGATCCACC CCAGACTACC CCATGCGCTT GTCTCCTGCC 
ACGCCTCGCT ATAGTGGACA CCATTCCCCA GCTCTTCTCA ACAAGAGTGC CGTGGCTCAG 
AGTTTCAAGC CGGAGCCTAA CACCACCCCA AAAATGCTTC CTGAGGGGGA TCACCAACAG 
ATGCCCGGTG TCTACAGTCC AGGGAGCACT CCTATTGCTC CCCTAAGCCC ACAGATTGGT 
TCTAGACCAA TGGATGGAGA AAAGGAAGAC TGGGAGCTGG ACACAGCAGA GAAACAGTTT 
GGTGCAGAGA CAACAGCTGC TACTCCTCCA CCACAGCTAT CCAATGGAAA CCCCAGCCAG 
GCAGTGCTGG AGTTCGCCCA GTACTTAGAC TCTCTATTCA GGCTGGACGG CAGCAATTCA 
CCGCCTCTGG AGCTGTCCGA CGGGGAGTCG GAGTCTGGCC GAGGCTCCTT TGGCCAGGGT 
GAATGCCTCG GAGATGGACA GCAGTTGGAC GACAAACAGC TTCTGGCCAG AGCAACACTG 
GAGCCAAATC TAGTCCCCAA GCCCCCACGC ACTTTTGCTG GATCTGCCGA CCCCTCTTCG 
GGCATATCTT TGTCACCTTC CTTCCCTCTC TCATCGTCTG AAGAGCTCAA TGACCTCTCC 
CCCAGTGAGG GTGCTCCGCC AGCCATACAC TCCCTACGAA CATCTTCTCC TTGCCACTGT 
CCTGAGGAGA ACACACTATC ATCTATGGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dagla ( XM_023279289.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023135057.1 Amphiprion ocellaris diacylglycerol lipase alpha (dagla), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAm84993
Clone ID Related Accession (Same CDS sequence)	XM_023279289.1
Accession Version	XM_023279289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	sn1-specific diacylglycerol lipase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019372821.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAA TGGTGATGTT TCGGCGGCGC TGGTCAGTTG GCAGTGATGA CCTGGTGCTG 
CCCGCCCTCT TCCTTTTCTT ATTACACTGC ATCTGGTTGG TGGTTCTGTC CGTGGTTCTC 
TTTGGCCTTC CATATGGCTC AGACCAGTCC TGCTCTGTAA CACTGGTGGA CCATGGCCGA 
GGGTACCTGG GCATCCTGGT CAGCTGCCTT ATATGTGAGA GCGCCATCAT GTGGTTGAGT 
ATGAGGGGCA GCATTCTGTA CACACAGCCC AGGGAAGCTG TGCAATATGT CATCTATATA 
CGACTAGCTA TTCTGTTGGT TGAGCTAGTA TATGCTGTAG TAGGAATTGC CTGGCTTGTC 
CAGTATTACC AGCCGTGCTC TGATGTCACT GCCAAAAACT TGGCTTTGGG AATTGTTGCA 
TGCAACTGGC TAGTGATATT CAGCGTTTGC ATCACGCTCA TGTGCACCTT TGATCCTACG 
GGTCGGACTT TTGTCAAACT GAAAGCCACC CGTCGGCGTC AGCGTAACCT CACTACATAC 
ACACTCAGAC ACAGGCTAGA GGAAGGCCAA GCCAGTAGCT GGAGCAGGAG ACTAAAGTTC 
TTCATGTGCT GCACAAGAAC CCAAGATACA CAATCAGATG CGTATTCAGA AGTGGCCAGC 
CTTTTTGCAG AGTTCTTCAG AGACCTGGAT ATTGTGCCTA GTGATATTAT TGCTGGCCTG 
GTACTTCTCC GCCAGAGGCA AAGGTCTAAG AGATCTGCTA TCCTGGACCA GGCCAACAAT 
GACATTTTAG CCTTCTTATC AGGAATGCCT GTCACTCGAA ACACTAGATA TTTGGACCTG 
AAGAACTCGG CTGAGATGAA CATGTACAAA GATGTGTGTT ATTATATGCT CTTTGCCCTG 
GCTGCATATG GTTGGCCCAT GTACCTGATG AGGAAGCCTG CCTGTGGGCT GTGCCGCCTC 
GCCAGCTCTT GCCCCTGTAC ATCAGTGTCT GGCTCTCGGT TGTCTCAGTC TGTGACAGTG 
GAGGAGGACA ACTGTTGCGG CTGTAACGTC CTGGCCATAC GTAGACACTT CCTGGACAGG 
GATCTCAAGC AGGTTCACAT TGTCTACACA TCCTGCCACG ATGCTGTTTA TGAAACTCCA 
TTTTTTGTGG CAGTTGATCA CGCGAAAAAG AAAGTTGTCA TCAGTATCAG AGGAACCCTT 
TCACCAAAGG ATGCCTTGAC TGATCTGACA GGAGACTCTG AACGTTTGCC TGTGGAGGAG 
CAACATGGGA CTTGGCTCGG CCACAAGGGA ATGGTTTACT CAGCAGAATA CATTAAGAAG 
AAACTGGAGC AGGAAATGAT CCTGTCACAA GCGTTTGGAA GAGACTTGAA CAAGGGCACC 
ATGCACTATG GGCTGGTGAT AGTTGGGCAT TCTCTTGGTG CAGGCACTGC TACTATCCTC 
TCCTTCCTGC TGAGACCTCA GTACCCTACA CTTCACTGCT ACTCTTATTC TCCACCTGGT 
GGCCTTCTTA GTGAGGATGC CATGGAGTAC TCCAAAGAGT TCGTCACGTC CGTGGTATTA 
GGAAAAGACC TGGTACCAAG GCTGGGCCTG TCACAACTGG AGGGTTTCCG ACGCCACCTT 
CTGGAAGTCT TACAGAAAAG TAACAAGCCA AAGTGGAGGA TCATTGCAGG AGGCACTAAA 
TGCATTCCAA AGTCAGAACT TCCACTAGAG GATGATGATC CTCAGTCTCA GCCGGCAGCA 
CCCCCCAGCA GTCGCCTGTG GCTGCATCCC AGTGACCTCA GCATTGCCCT GTCAGCCTCC 
ACACCCCTCT TCCCTCCTGG CAGAATCATC CACGTGGTGC ACAACCATCC TCCAGAGACA 
TGCTGTGGCC AGGAAGAGCC GACCTATTCA GCTCTGTGGG GGGACAACAA GGCCTTCAAT 
GAGGTCATAA TATCACCCGC CATGCTTAAT GAGCACATGC CCCACATGGT GATGGAGGGC 
CTAAACAAGG TTCTGGAGAA CTATAACAAA GGGAAAACTG CTCTGCTGTC AGCAGCCAAG 
ATCATGGTGA GCCCCACTGA AGTGGACTTG AACCCAGAGA CCATGTTCGT GGATACATCA 
ACAACCTCTC AGCAGCCGAC GCCTACAACC CACCACCGCA GGAACAGTAG TGTTCGTTCC 
TGTGCCCAGT CTGAAATATC TCTGGACGGT TTCTCTGAGT GCCCTCCTCC CCCAGTGCCT 
GTAGTGCTGC GTGGAGCACG GGAGCGCCTG GCAGTGGAGC TGAGGGACCG CAAAGCACCG 
CTGGCCGTCA TGGAGAGCCT CTCAGACGCC GAGTCTCTCT ACAGTCTGGA TTCCCGACGC 
TCCTCAGCTG CGTTAAGGGG TTCGCCAATG CTGGGTAGCC TTCCATTCCC TTTGGATGCC 
CCGATCCCAG AGGAGAACCC GTCTCTCAGC TCTCGCACTG AACTGCTGGC TGCGGATTGC 
CTCTGCCAGG AAACACCAGA ACACCTTGGT GAGGTTGGGC CCTTCTTTAC CCCACTGGAA 
TCCAGATCCA CCCCAGACTA CCCCATGCGC TTGTCTCCTG CCACGCCTCG CTATAGTGGA 
CACCATTCCC CAGCTCTTCT CAACAAGAGT GCCGTGGCTC AGAGTTTCAA GCCGGAGCCT 
AACACCACCC CAAAAATGCT TCCTGAGGGG GATCACCAAC AGATGCCCGG TGTCTACAGT 
CCAGGGAGCA CTCCTATTGC TCCCCTAAGC CCACAGATTG GTTCTAGACC AATGGATGGA 
GAAAAGGAAG ACTGGGAGCT GGACACAGCA GAGAAACAGT TTGGTGCAGA GACAACAGCT 
GCTACTCCTC CACCACAGCT ATCCAATGGA AACCCCAGCC AGGCAGTGCT GGAGTTCGCC 
CAGTACTTAG ACTCTCTATT CAGGCTGGAC GGCAGCAATT CACCGCCTCT GGAGCTGTCC 
GACGGGGAGT CGGAGTCTGG CCGAGGCTCC TTTGGCCAGG GTGAATGCCT CGGAGATGGA 
CAGCAGTTGG ACGACAAACA GCTTCTGGCC AGAGCAACAC TGGAGCCAAA TCTAGTCCCC 
AAGCCCCCAC GCACTTTTGC TGGATCTGCC GACCCCTCTT CGGGCATATC TTTGTCACCT 
TCCTTCCCTC TCTCATCGTC TGAAGAGCTC AATGACCTCT CCCCCAGTGA GGGTGCTCCG 
CCAGCCATAC ACTCCCTACG AACATCTTCT CCTTGCCACT GTCCTGAGGA GAACACACTA 
TCATCTATGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023135057.1
CDS	923..4177
Translation	MPGMVMFRRRWSVGSDDLVLPALFLFLLHCIWLVVLSVVLFGLPYGSDQSCSVTLVDHGRGYLGILVSCLICESAIMWLSMRGSILYTQPREAVQYVIYIRLAILLVELVYAVVGIAWLVQYYQPCSDVTAKNLALGIVACNWLVIFSVCITLMCTFDPTGRTFVKLKATRRRQRNLTTYTLRHRLEEGQASSWSRRLKFFMCCTRTQDTQSDAYSEVASLFAEFFRDLDIVPSDIIAGLVLLRQRQRSKRSAILDQANNDILAFLSGMPVTRNTRYLDLKNSAEMNMYKDVCYYMLFALAAYGWPMYLMRKPACGLCRLASSCPCTSVSGSRLSQSVTVEEDNCCGCNVLAIRRHFLDRDLKQVHIVYTSCHDAVYETPFFVAVDHAKKKVVISIRGTLSPKDALTDLTGDSERLPVEEQHGTWLGHKGMVYSAEYIKKKLEQEMILSQAFGRDLNKGTMHYGLVIVGHSLGAGTATILSFLLRPQYPTLHCYSYSPPGGLLSEDAMEYSKEFVTSVVLGKDLVPRLGLSQLEGFRRHLLEVLQKSNKPKWRIIAGGTKCIPKSELPLEDDDPQSQPAAPPSSRLWLHPSDLSIALSASTPLFPPGRIIHVVHNHPPETCCGQEEPTYSALWGDNKAFNEVIISPAMLNEHMPHMVMEGLNKVLENYNKGKTALLSAAKIMVSPTEVDLNPETMFVDTSTTSQQPTPTTHHRRNSSVRSCAQSEISLDGFSECPPPPVPVVLRGARERLAVELRDRKAPLAVMESLSDAESLYSLDSRRSSAALRGSPMLGSLPFPLDAPIPEENPSLSSRTELLAADCLCQETPEHLGEVGPFFTPLESRSTPDYPMRLSPATPRYSGHHSPALLNKSAVAQSFKPEPNTTPKMLPEGDHQQMPGVYSPGSTPIAPLSPQIGSRPMDGEKEDWELDTAEKQFGAETTAATPPPQLSNGNPSQAVLEFAQYLDSLFRLDGSNSPPLELSDGESESGRGSFGQGECLGDGQQLDDKQLLARATLEPNLVPKPPRTFAGSADPSSGISLSPSFPLSSSEELNDLSPSEGAPPAIHSLRTSSPCHCPEENTLSSMV

Target ORF information:

RefSeq Version	XM_023279289.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris diacylglycerol lipase alpha (dagla), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023279289.1

ORF Insert Sequence:

ATGCCTGGAA TGGTGATGTT TCGGCGGCGC TGGTCAGTTG GCAGTGATGA CCTGGTGCTG 
CCCGCCCTCT TCCTTTTCTT ATTACACTGC ATCTGGTTGG TGGTTCTGTC CGTGGTTCTC 
TTTGGCCTTC CATATGGCTC AGACCAGTCC TGCTCTGTAA CACTGGTGGA CCATGGCCGA 
GGGTACCTGG GCATCCTGGT CAGCTGCCTT ATATGTGAGA GCGCCATCAT GTGGTTGAGT 
ATGAGGGGCA GCATTCTGTA CACACAGCCC AGGGAAGCTG TGCAATATGT CATCTATATA 
CGACTAGCTA TTCTGTTGGT TGAGCTAGTA TATGCTGTAG TAGGAATTGC CTGGCTTGTC 
CAGTATTACC AGCCGTGCTC TGATGTCACT GCCAAAAACT TGGCTTTGGG AATTGTTGCA 
TGCAACTGGC TAGTGATATT CAGCGTTTGC ATCACGCTCA TGTGCACCTT TGATCCTACG 
GGTCGGACTT TTGTCAAACT GAAAGCCACC CGTCGGCGTC AGCGTAACCT CACTACATAC 
ACACTCAGAC ACAGGCTAGA GGAAGGCCAA GCCAGTAGCT GGAGCAGGAG ACTAAAGTTC 
TTCATGTGCT GCACAAGAAC CCAAGATACA CAATCAGATG CGTATTCAGA AGTGGCCAGC 
CTTTTTGCAG AGTTCTTCAG AGACCTGGAT ATTGTGCCTA GTGATATTAT TGCTGGCCTG 
GTACTTCTCC GCCAGAGGCA AAGGTCTAAG AGATCTGCTA TCCTGGACCA GGCCAACAAT 
GACATTTTAG CCTTCTTATC AGGAATGCCT GTCACTCGAA ACACTAGATA TTTGGACCTG 
AAGAACTCGG CTGAGATGAA CATGTACAAA GATGTGTGTT ATTATATGCT CTTTGCCCTG 
GCTGCATATG GTTGGCCCAT GTACCTGATG AGGAAGCCTG CCTGTGGGCT GTGCCGCCTC 
GCCAGCTCTT GCCCCTGTAC ATCAGTGTCT GGCTCTCGGT TGTCTCAGTC TGTGACAGTG 
GAGGAGGACA ACTGTTGCGG CTGTAACGTC CTGGCCATAC GTAGACACTT CCTGGACAGG 
GATCTCAAGC AGGTTCACAT TGTCTACACA TCCTGCCACG ATGCTGTTTA TGAAACTCCA 
TTTTTTGTGG CAGTTGATCA CGCGAAAAAG AAAGTTGTCA TCAGTATCAG AGGAACCCTT 
TCACCAAAGG ATGCCTTGAC TGATCTGACA GGAGACTCTG AACGTTTGCC TGTGGAGGAG 
CAACATGGGA CTTGGCTCGG CCACAAGGGA ATGGTTTACT CAGCAGAATA CATTAAGAAG 
AAACTGGAGC AGGAAATGAT CCTGTCACAA GCGTTTGGAA GAGACTTGAA CAAGGGCACC 
ATGCACTATG GGCTGGTGAT AGTTGGGCAT TCTCTTGGTG CAGGCACTGC TACTATCCTC 
TCCTTCCTGC TGAGACCTCA GTACCCTACA CTTCACTGCT ACTCTTATTC TCCACCTGGT 
GGCCTTCTTA GTGAGGATGC CATGGAGTAC TCCAAAGAGT TCGTCACGTC CGTGGTATTA 
GGAAAAGACC TGGTACCAAG GCTGGGCCTG TCACAACTGG AGGGTTTCCG ACGCCACCTT 
CTGGAAGTCT TACAGAAAAG TAACAAGCCA AAGTGGAGGA TCATTGCAGG AGGCACTAAA 
TGCATTCCAA AGTCAGAACT TCCACTAGAG GATGATGATC CTCAGTCTCA GCCGGCAGCA 
CCCCCCAGCA GTCGCCTGTG GCTGCATCCC AGTGACCTCA GCATTGCCCT GTCAGCCTCC 
ACACCCCTCT TCCCTCCTGG CAGAATCATC CACGTGGTGC ACAACCATCC TCCAGAGACA 
TGCTGTGGCC AGGAAGAGCC GACCTATTCA GCTCTGTGGG GGGACAACAA GGCCTTCAAT 
GAGGTCATAA TATCACCCGC CATGCTTAAT GAGCACATGC CCCACATGGT GATGGAGGGC 
CTAAACAAGG TTCTGGAGAA CTATAACAAA GGGAAAACTG CTCTGCTGTC AGCAGCCAAG 
ATCATGGTGA GCCCCACTGA AGTGGACTTG AACCCAGAGA CCATGTTCGT GGATACATCA 
ACAACCTCTC AGCAGCCGAC GCCTACAACC CACCACCGCA GGAACAGTAG TGTTCGTTCC 
TGTGCCCAGT CTGAAATATC TCTGGACGGT TTCTCTGAGT GCCCTCCTCC CCCAGTGCCT 
GTAGTGCTGC GTGGAGCACG GGAGCGCCTG GCAGTGGAGC TGAGGGACCG CAAAGCACCG 
CTGGCCGTCA TGGAGAGCCT CTCAGACGCC GAGTCTCTCT ACAGTCTGGA TTCCCGACGC 
TCCTCAGCTG CGTTAAGGGG TTCGCCAATG CTGGGTAGCC TTCCATTCCC TTTGGATGCC 
CCGATCCCAG AGGAGAACCC GTCTCTCAGC TCTCGCACTG AACTGCTGGC TGCGGATTGC 
CTCTGCCAGG AAACACCAGA ACACCTTGGT GAGGTTGGGC CCTTCTTTAC CCCACTGGAA 
TCCAGATCCA CCCCAGACTA CCCCATGCGC TTGTCTCCTG CCACGCCTCG CTATAGTGGA 
CACCATTCCC CAGCTCTTCT CAACAAGAGT GCCGTGGCTC AGAGTTTCAA GCCGGAGCCT 
AACACCACCC CAAAAATGCT TCCTGAGGGG GATCACCAAC AGATGCCCGG TGTCTACAGT 
CCAGGGAGCA CTCCTATTGC TCCCCTAAGC CCACAGATTG GTTCTAGACC AATGGATGGA 
GAAAAGGAAG ACTGGGAGCT GGACACAGCA GAGAAACAGT TTGGTGCAGA GACAACAGCT 
GCTACTCCTC CACCACAGCT ATCCAATGGA AACCCCAGCC AGGCAGTGCT GGAGTTCGCC 
CAGTACTTAG ACTCTCTATT CAGGCTGGAC GGCAGCAATT CACCGCCTCT GGAGCTGTCC 
GACGGGGAGT CGGAGTCTGG CCGAGGCTCC TTTGGCCAGG GTGAATGCCT CGGAGATGGA 
CAGCAGTTGG ACGACAAACA GCTTCTGGCC AGAGCAACAC TGGAGCCAAA TCTAGTCCCC 
AAGCCCCCAC GCACTTTTGC TGGATCTGCC GACCCCTCTT CGGGCATATC TTTGTCACCT 
TCCTTCCCTC TCTCATCGTC TGAAGAGCTC AATGACCTCT CCCCCAGTGA GGGTGCTCCG 
CCAGCCATAC ACTCCCTACG AACATCTTCT CCTTGCCACT GTCCTGAGGA GAACACACTA 
TCATCTATGG TGTAG