spred1 cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	spred1
Entrez Gene ID	111573272
Full Name	sprouty related EVH1 domain containing 1
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023277348.1	XP_023133116.1	sprouty-related, EVH1 domain-containing protein 1

Pathways from BioSystems
Gene Ontology

The following spred1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spred1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm83460	XM_023277348.1 Latest version!	Amphiprion ocellaris sprouty related EVH1 domain containing 1 (spred1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spred1 ( XM_023277348.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023133116.1 Amphiprion ocellaris sprouty related EVH1 domain containing 1 (spred1), mRNA.

Price & Availability↑

CloneID	OAm83460
Clone ID Related Accession (Same CDS sequence)	XM_023277348.1
Accession Version	XM_023277348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	sprouty-related, EVH1 domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019372735.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAG ATTCAACAAA TCCAAACAAC GATGACAGTT ATGCGCGTGT GAGAGCAGTG 
GTCATGACTC GGGATGACTC CAGTGGTGGG TGGCTTGCCC TGGGGGGTGG AGGCCTTAGC 
TGTGTCACCG TTTATAAGGT CAGCCGGGCG GAGGACAGCA GCAGCACCCA CAGTGGAGGC 
AGCGGAGGAA GCAGCGGCAA CCTCAGCCCC TGTAGCCCCA CCCCCAGTCC CAGTCCCACC 
CCTAGTCCCA GTCCTACTGC TGTGGAGTTC CACATCAAGG GCGAAAGGCT CAAAGACAAA 
CTGGTGGTAC TGGAATGTGT TCTGCAGAAA GACCTAGTCT ACAACAAAGT CAACCCCATC 
TTCCACCACT GGAGGATCAA TGACAAGAAG TTTGGACTGA CCTTCCAGAG TCCTGCTGAC 
GCCCGCGCCT TTGACCGGGG CATACGCCGG GCCATTGAGG ACATCCACCA AGGTTGTCGG 
CCTTTTGGAG AAGGGGATGC TCCTGACGAT GGACTACCGG TGTGTGATGA GCCTCCCTCC 
ATCTGTACCC CAATGAAAGA GCCCTTCTCT CCGCTGAACA ATGTTGTCTC CACCGAGCCA 
TTCAGGGGCT GCTACGTACG TGCCCAGCCC TTCGACGAGT TCCCCTCCAG CAACCGCCGC 
TACCTGCCTC CACAGTCGAC TCGCCGTGTC AGTTTTCGTA TGGACGAAGA GGAGATTGTT 
CGCATCAACC CACGCAAAGA CGTGCTCATC CGTGGCTACG AGGACTACCG CCACCCTGTC 
ATGTGGAAAC AAGAGGCCGA CCGTGAGGAC CTGGACTTCC CCACCGCCTT TCACAAACTG 
GACAGTAAGA AGTGCGAGTA CCTCTTCCCC GACGGCCCTG GTGGGGACTC CCACTCTGGC 
CCTGGTATGG GCATTGGAAC AGGTATGGGG CTCGGTCTGG GCAAGGACAC AGCCATCAAG 
ACTCAGCCGT CGCCCCTGCT GAAATCCAAG AAGGGCCGTC GGCGACGAGA GGACGGCGAA 
CGTTCGCGCT GCATCTACTG TCGGGAGATG TTCAACCACG AAGACAACTG GCGGGGGCAG 
TGCCAGGATG CCCCCGACCC CATTAAGCAG TGCATCTACA AAGTCAGCTG CATGCTGTGT 
GCCGAGAGCA TGCTGTATCA CTGCATGTCT GACTCGGAGG GTGACTTCTC AGACCCCTGC 
TCTTGTGACA CATCTGACGA GCAGTTCTGT CTGCGCTGGC TGGCCCTGGT GGCGCTCTCC 
TTCATTGCGC CCTGCATGTG CTGCTACCTG CCTCTGCGCG CCTGCCATCA CTGTGGCGAG 
GCCTGCCGCT GCTGTGGGGG CAAGCACAAG GCTGCGGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023133116.1
CDS	787..2148
Translation	MSEDSTNPNNDDSYARVRAVVMTRDDSSGGWLALGGGGLSCVTVYKVSRAEDSSSTHSGGSGGSSGNLSPCSPTPSPSPTPSPSPTAVEFHIKGERLKDKLVVLECVLQKDLVYNKVNPIFHHWRINDKKFGLTFQSPADARAFDRGIRRAIEDIHQGCRPFGEGDAPDDGLPVCDEPPSICTPMKEPFSPLNNVVSTEPFRGCYVRAQPFDEFPSSNRRYLPPQSTRRVSFRMDEEEIVRINPRKDVLIRGYEDYRHPVMWKQEADREDLDFPTAFHKLDSKKCEYLFPDGPGGDSHSGPGMGIGTGMGLGLGKDTAIKTQPSPLLKSKKGRRRREDGERSRCIYCREMFNHEDNWRGQCQDAPDPIKQCIYKVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDEQFCLRWLALVALSFIAPCMCCYLPLRACHHCGEACRCCGGKHKAAG

Target ORF information:

RefSeq Version	XM_023277348.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris sprouty related EVH1 domain containing 1 (spred1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023277348.1

ORF Insert Sequence:

ATGAGTGAAG ATTCAACAAA TCCAAACAAC GATGACAGTT ATGCGCGTGT GAGAGCAGTG 
GTCATGACTC GGGATGACTC CAGTGGTGGG TGGCTTGCCC TGGGGGGTGG AGGCCTTAGC 
TGTGTCACCG TTTATAAGGT CAGCCGGGCG GAGGACAGCA GCAGCACCCA CAGTGGAGGC 
AGCGGAGGAA GCAGCGGCAA CCTCAGCCCC TGTAGCCCCA CCCCCAGTCC CAGTCCCACC 
CCTAGTCCCA GTCCTACTGC TGTGGAGTTC CACATCAAGG GCGAAAGGCT CAAAGACAAA 
CTGGTGGTAC TGGAATGTGT TCTGCAGAAA GACCTAGTCT ACAACAAAGT CAACCCCATC 
TTCCACCACT GGAGGATCAA TGACAAGAAG TTTGGACTGA CCTTCCAGAG TCCTGCTGAC 
GCCCGCGCCT TTGACCGGGG CATACGCCGG GCCATTGAGG ACATCCACCA AGGTTGTCGG 
CCTTTTGGAG AAGGGGATGC TCCTGACGAT GGACTACCGG TGTGTGATGA GCCTCCCTCC 
ATCTGTACCC CAATGAAAGA GCCCTTCTCT CCGCTGAACA ATGTTGTCTC CACCGAGCCA 
TTCAGGGGCT GCTACGTACG TGCCCAGCCC TTCGACGAGT TCCCCTCCAG CAACCGCCGC 
TACCTGCCTC CACAGTCGAC TCGCCGTGTC AGTTTTCGTA TGGACGAAGA GGAGATTGTT 
CGCATCAACC CACGCAAAGA CGTGCTCATC CGTGGCTACG AGGACTACCG CCACCCTGTC 
ATGTGGAAAC AAGAGGCCGA CCGTGAGGAC CTGGACTTCC CCACCGCCTT TCACAAACTG 
GACAGTAAGA AGTGCGAGTA CCTCTTCCCC GACGGCCCTG GTGGGGACTC CCACTCTGGC 
CCTGGTATGG GCATTGGAAC AGGTATGGGG CTCGGTCTGG GCAAGGACAC AGCCATCAAG 
ACTCAGCCGT CGCCCCTGCT GAAATCCAAG AAGGGCCGTC GGCGACGAGA GGACGGCGAA 
CGTTCGCGCT GCATCTACTG TCGGGAGATG TTCAACCACG AAGACAACTG GCGGGGGCAG 
TGCCAGGATG CCCCCGACCC CATTAAGCAG TGCATCTACA AAGTCAGCTG CATGCTGTGT 
GCCGAGAGCA TGCTGTATCA CTGCATGTCT GACTCGGAGG GTGACTTCTC AGACCCCTGC 
TCTTGTGACA CATCTGACGA GCAGTTCTGT CTGCGCTGGC TGGCCCTGGT GGCGCTCTCC 
TTCATTGCGC CCTGCATGTG CTGCTACCTG CCTCTGCGCG CCTGCCATCA CTGTGGCGAG 
GCCTGCCGCT GCTGTGGGGG CAAGCACAAG GCTGCGGGGT GA