bcar1 cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	bcar1
Entrez Gene ID	111566681
Full Name	BCAR1, Cas family scaffolding protein
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023267416.1	XP_023123184.1	breast cancer anti-estrogen resistance protein 1 isoform X2
XM_023267415.1	XP_023123183.1	breast cancer anti-estrogen resistance protein 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following bcar1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bcar1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm75639	XM_023267415.1 Latest version!	Amphiprion ocellaris BCAR1, Cas family scaffolding protein (bcar1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAm75640	XM_023267416.1 Latest version!	Amphiprion ocellaris BCAR1, Cas family scaffolding protein (bcar1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bcar1 ( XM_023267415.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023123183.1 Amphiprion ocellaris BCAR1, Cas family scaffolding protein (bcar1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAm75639
Clone ID Related Accession (Same CDS sequence)	XM_023267415.1
Accession Version	XM_023267415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	breast cancer anti-estrogen resistance protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019371841.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTATC TGAACGTGTT GGCCAAGGCC TTGTATGACA ACGTGGCTGA GTCTCCAGAT 
GAACTGTCCT TCCGCAAAGG TGACATCATG ACAGTGTTGG AGCGTGACAC ACAGGGATTG 
GATGGCTGGT GGCTCTGCTC ACTCCACGGA CGCCAAGGCA TCGTCCCTGG CAATCGTCTG 
AAGATTCTGG TGGGCATGTA TGACAGTAAG CAGCAGCAGC AGGCCACTCC TCCCACACCA 
GATCTACTTG TTTCCTGTTC TGCCTCCCAG GTTCAGCGAC CCCTCCCCAT TCAAAGCACC 
TACGCCAAAC CAGCACCTGC TTTGTCATCT GCTGCCATCG CTACGTCCTC TGGGTTTGCC 
ACCAAGCCCC TCCCCTCAAC ACAGTACACG TCCATGCACC CAGCCTACTC CACCCCCAGC 
CCTGCCCAGC CCAACCCTGA CTCTGTCTAC ATGATGCCGC CCTCCCATGG TTCAAAACCA 
AGCCCCCAAA GTCTATACCA GGTTCCCTCT GGTCCAAGTG GACCCTCTGC ACAGGCACAG 
CCAGGACCCC CAAGCAAGGC TCCACCTCTA GGACAAAGAC AGTACCAGCT CCCTGGACAG 
GATATTTATC AGGTGCCCCC CTGTGGAGGC CCAGGACCAG GCCATGGAGC AGTTCCATCT 
GGGGGTACAG GAGCTGTCCA GGATGTGTAC CAGGTGCCCC CCACTCTGGA TAAGAGGAAC 
TGGGAGAGCA GCAACAAGCC TCTTGGCAAG GTGGTTGTTC CTACCCGTGT TGGACAGGTG 
TATGTATACG ACACAGTAAA ACCAGACCGG GACGAGTACG ACATCCCACC AAGACATCAG 
CCCCCAACCC AGCAGGACAT ATATGACGTG CCTCCCACCC GCCAGCAGTA CAGTACACAG 
GTGTATGATA CTCCTCCCAT GGTGGTGAAG GATCCCTCTA GTGGTCAAGA TATATATGAC 
ATCCCAGCAA GCACAGACAA GAACCCACAA CAGACTGTCT ATGACTTCCC ACCATCTGTC 
AGTAAAGACA TACCTGTTTC TCAACCAATA AGAGAGGAGA CCTATGATGT GCCACCCCAC 
TTTGCCAAAA TAAAGTCCCA AGTCGCCATC ACTCCTGGCC AGTACCTGCA GAACAACATC 
AATGACGATG ACGAAGAGCC GCCGATTCCA GAGGATGTTT ACGACGTCCC ACCCCCTATC 
CTGACTGACA AGCATTATCA CAGAGACAGG AATGGATTCA GCCAGGCCCC CCAGGAGATC 
TATGACATCC CAGCCATTCT GCATCCTGGA GGGTACCCCA CCAAGGATGT ATATGACTTC 
CCCCGAGAAC GAGAGGAACA AGGAGGAGAG AGGGGAGGCC AGTATGTCTA TGATGTCCCA 
CCACAGGTTG TTCGTGATGC CCAGTCAACC AGTGAAGAGC TGACAATGTC TTTCAAGCGG 
CTGTCTGCCT CAAGCACAGG AAGCACACGG AGCAACCACT CTACTTCATC ATTAGACATT 
GTCCCTGTCC GTGACACCTC CTCACTTCCT GCTCCAAGCC CCATTCCAGG GAAACCACTC 
ATCTTGGACC TGGATCAGGC CATGGAGCGT CTGTCTCGCC TCCAGCAAGC TGTTGAGTCC 
AGTGTTTCCT TCATGATGTC ATTCATTACA GGCAATTGGA GAAACCCTGC TCACCTGGAG 
GGGAATCTTC CGGCTATTCA TCAGGCTGCT GACCGGGTGC GAGTCACTGT TCGAGACTTT 
CTAGAATTTG CTCGGGGTGC TGTGGCCAAT GCTACCCAGG TTACAGACCG CTCACTACAG 
ACCAAACTAG GCCGTCAGGT GGGAAAGATG GAGGAGGTCT TCCAGAGTTT GATTCGACAC 
AGTCAGAGCT TAGACACAAT TTCTTGGTCA CACACAGCAC TCACAACACC ACCACCAGGA 
GGGGATGACT TAGATCTACT AATTATGACA GCGCGAGGCA TCCCCGATGA CGCCAAGCAG 
CTTGCCTCCT TTCTCCATGG TAATGCCTCA CTGCTCTTCA AACGGACGAC CCGGCAACAG 
CAGCAGCTGC CTCTTCCTCC GATCCCTGGG GAGATCAGTG GTCACATGAC AGGGTCAGGA 
AGTGGAAGCT TTCAGGGAGG GGAGAAGGTG AATATTCAGT CACGGCCCCT CCCCTCTCCA 
CCAAAATTTA CAGCTGCAGA GGACGAGGAA GGTGTGGACA GGCCGTATGA GACCACAGAG 
GAAGGCTGGA TGGAAGACTA TGACTACGTA CATCTACAGG GAAAGGAAGA GTTTGAGAAG 
AACCAAAGAC AACTGCTGGA AAAAGGCAAT ATTATCAGAC ACAAGAAGAC ACAACTGGAA 
CAGCAACAGA TTAAACAGTT TGAGAGGCTA GAGCAGGAAG TCAGCCGGCC AATCAACAAT 
GACATGACGG GCTGGGTTCC ATCCCCACAT CACCCTCTAG CCAACCAGCT GGCCCAGAAC 
GGCTCTGGAG GCTCCTCCTC CTCCAAGCTG TGCCATGGCG ACCAGCAGCT CCTCCTCTTC 
TATCAGGAGC AATGTGAACA GAATGTCACC ACAGTGACCA ACGCCATTGA TGCTTTCTTT 
ACGGCCATCA ACTCTAACCA GCCACCAAAG ATCTTTGTGG CACACAGCAA ATTTGTCATT 
CTCAGTGCAC ATAAGCTGGT GTTCATTGGC GACACACTGT CACGTCAGGC CAAGACTCCT 
GAGGTGAGGG CCCGGGTTGC CCAAAGCAGC AACACCCTCT GTGAAAAACT CAAAGACATT 
GTGATCAGTA CAAAGACAGC AGCGCTTCAA TATCCTTCGC CTGGAGCAGC CAGAGAGATG 
ACTGAGCGGG TAAAGGAGCT GGCAGGATAT ACACAGCAGT TCAGGATGGT GCTGGGACAA 
CTGCTGGTGA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023123183.1
CDS	320..3214
Translation	MNYLNVLAKALYDNVAESPDELSFRKGDIMTVLERDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDSKQQQQATPPTPDLLVSCSASQVQRPLPIQSTYAKPAPALSSAAIATSSGFATKPLPSTQYTSMHPAYSTPSPAQPNPDSVYMMPPSHGSKPSPQSLYQVPSGPSGPSAQAQPGPPSKAPPLGQRQYQLPGQDIYQVPPCGGPGPGHGAVPSGGTGAVQDVYQVPPTLDKRNWESSNKPLGKVVVPTRVGQVYVYDTVKPDRDEYDIPPRHQPPTQQDIYDVPPTRQQYSTQVYDTPPMVVKDPSSGQDIYDIPASTDKNPQQTVYDFPPSVSKDIPVSQPIREETYDVPPHFAKIKSQVAITPGQYLQNNINDDDEEPPIPEDVYDVPPPILTDKHYHRDRNGFSQAPQEIYDIPAILHPGGYPTKDVYDFPREREEQGGERGGQYVYDVPPQVVRDAQSTSEELTMSFKRLSASSTGSTRSNHSTSSLDIVPVRDTSSLPAPSPIPGKPLILDLDQAMERLSRLQQAVESSVSFMMSFITGNWRNPAHLEGNLPAIHQAADRVRVTVRDFLEFARGAVANATQVTDRSLQTKLGRQVGKMEEVFQSLIRHSQSLDTISWSHTALTTPPPGGDDLDLLIMTARGIPDDAKQLASFLHGNASLLFKRTTRQQQQLPLPPIPGEISGHMTGSGSGSFQGGEKVNIQSRPLPSPPKFTAAEDEEGVDRPYETTEEGWMEDYDYVHLQGKEEFEKNQRQLLEKGNIIRHKKTQLEQQQIKQFERLEQEVSRPINNDMTGWVPSPHHPLANQLAQNGSGGSSSSKLCHGDQQLLLFYQEQCEQNVTTVTNAIDAFFTAINSNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKTPEVRARVAQSSNTLCEKLKDIVISTKTAALQYPSPGAAREMTERVKELAGYTQQFRMVLGQLLVM

Target ORF information:

RefSeq Version	XM_023267415.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris BCAR1, Cas family scaffolding protein (bcar1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023267415.1

ORF Insert Sequence:

ATGAACTATC TGAACGTGTT GGCCAAGGCC TTGTATGACA ACGTGGCTGA GTCTCCAGAT 
GAACTGTCCT TCCGCAAAGG TGACATCATG ACAGTGTTGG AGCGTGACAC ACAGGGATTG 
GATGGCTGGT GGCTCTGCTC ACTCCACGGA CGCCAAGGCA TCGTCCCTGG CAATCGTCTG 
AAGATTCTGG TGGGCATGTA TGACAGTAAG CAGCAGCAGC AGGCCACTCC TCCCACACCA 
GATCTACTTG TTTCCTGTTC TGCCTCCCAG GTTCAGCGAC CCCTCCCCAT TCAAAGCACC 
TACGCCAAAC CAGCACCTGC TTTGTCATCT GCTGCCATCG CTACGTCCTC TGGGTTTGCC 
ACCAAGCCCC TCCCCTCAAC ACAGTACACG TCCATGCACC CAGCCTACTC CACCCCCAGC 
CCTGCCCAGC CCAACCCTGA CTCTGTCTAC ATGATGCCGC CCTCCCATGG TTCAAAACCA 
AGCCCCCAAA GTCTATACCA GGTTCCCTCT GGTCCAAGTG GACCCTCTGC ACAGGCACAG 
CCAGGACCCC CAAGCAAGGC TCCACCTCTA GGACAAAGAC AGTACCAGCT CCCTGGACAG 
GATATTTATC AGGTGCCCCC CTGTGGAGGC CCAGGACCAG GCCATGGAGC AGTTCCATCT 
GGGGGTACAG GAGCTGTCCA GGATGTGTAC CAGGTGCCCC CCACTCTGGA TAAGAGGAAC 
TGGGAGAGCA GCAACAAGCC TCTTGGCAAG GTGGTTGTTC CTACCCGTGT TGGACAGGTG 
TATGTATACG ACACAGTAAA ACCAGACCGG GACGAGTACG ACATCCCACC AAGACATCAG 
CCCCCAACCC AGCAGGACAT ATATGACGTG CCTCCCACCC GCCAGCAGTA CAGTACACAG 
GTGTATGATA CTCCTCCCAT GGTGGTGAAG GATCCCTCTA GTGGTCAAGA TATATATGAC 
ATCCCAGCAA GCACAGACAA GAACCCACAA CAGACTGTCT ATGACTTCCC ACCATCTGTC 
AGTAAAGACA TACCTGTTTC TCAACCAATA AGAGAGGAGA CCTATGATGT GCCACCCCAC 
TTTGCCAAAA TAAAGTCCCA AGTCGCCATC ACTCCTGGCC AGTACCTGCA GAACAACATC 
AATGACGATG ACGAAGAGCC GCCGATTCCA GAGGATGTTT ACGACGTCCC ACCCCCTATC 
CTGACTGACA AGCATTATCA CAGAGACAGG AATGGATTCA GCCAGGCCCC CCAGGAGATC 
TATGACATCC CAGCCATTCT GCATCCTGGA GGGTACCCCA CCAAGGATGT ATATGACTTC 
CCCCGAGAAC GAGAGGAACA AGGAGGAGAG AGGGGAGGCC AGTATGTCTA TGATGTCCCA 
CCACAGGTTG TTCGTGATGC CCAGTCAACC AGTGAAGAGC TGACAATGTC TTTCAAGCGG 
CTGTCTGCCT CAAGCACAGG AAGCACACGG AGCAACCACT CTACTTCATC ATTAGACATT 
GTCCCTGTCC GTGACACCTC CTCACTTCCT GCTCCAAGCC CCATTCCAGG GAAACCACTC 
ATCTTGGACC TGGATCAGGC CATGGAGCGT CTGTCTCGCC TCCAGCAAGC TGTTGAGTCC 
AGTGTTTCCT TCATGATGTC ATTCATTACA GGCAATTGGA GAAACCCTGC TCACCTGGAG 
GGGAATCTTC CGGCTATTCA TCAGGCTGCT GACCGGGTGC GAGTCACTGT TCGAGACTTT 
CTAGAATTTG CTCGGGGTGC TGTGGCCAAT GCTACCCAGG TTACAGACCG CTCACTACAG 
ACCAAACTAG GCCGTCAGGT GGGAAAGATG GAGGAGGTCT TCCAGAGTTT GATTCGACAC 
AGTCAGAGCT TAGACACAAT TTCTTGGTCA CACACAGCAC TCACAACACC ACCACCAGGA 
GGGGATGACT TAGATCTACT AATTATGACA GCGCGAGGCA TCCCCGATGA CGCCAAGCAG 
CTTGCCTCCT TTCTCCATGG TAATGCCTCA CTGCTCTTCA AACGGACGAC CCGGCAACAG 
CAGCAGCTGC CTCTTCCTCC GATCCCTGGG GAGATCAGTG GTCACATGAC AGGGTCAGGA 
AGTGGAAGCT TTCAGGGAGG GGAGAAGGTG AATATTCAGT CACGGCCCCT CCCCTCTCCA 
CCAAAATTTA CAGCTGCAGA GGACGAGGAA GGTGTGGACA GGCCGTATGA GACCACAGAG 
GAAGGCTGGA TGGAAGACTA TGACTACGTA CATCTACAGG GAAAGGAAGA GTTTGAGAAG 
AACCAAAGAC AACTGCTGGA AAAAGGCAAT ATTATCAGAC ACAAGAAGAC ACAACTGGAA 
CAGCAACAGA TTAAACAGTT TGAGAGGCTA GAGCAGGAAG TCAGCCGGCC AATCAACAAT 
GACATGACGG GCTGGGTTCC ATCCCCACAT CACCCTCTAG CCAACCAGCT GGCCCAGAAC 
GGCTCTGGAG GCTCCTCCTC CTCCAAGCTG TGCCATGGCG ACCAGCAGCT CCTCCTCTTC 
TATCAGGAGC AATGTGAACA GAATGTCACC ACAGTGACCA ACGCCATTGA TGCTTTCTTT 
ACGGCCATCA ACTCTAACCA GCCACCAAAG ATCTTTGTGG CACACAGCAA ATTTGTCATT 
CTCAGTGCAC ATAAGCTGGT GTTCATTGGC GACACACTGT CACGTCAGGC CAAGACTCCT 
GAGGTGAGGG CCCGGGTTGC CCAAAGCAGC AACACCCTCT GTGAAAAACT CAAAGACATT 
GTGATCAGTA CAAAGACAGC AGCGCTTCAA TATCCTTCGC CTGGAGCAGC CAGAGAGATG 
ACTGAGCGGG TAAAGGAGCT GGCAGGATAT ACACAGCAGT TCAGGATGGT GCTGGGACAA 
CTGCTGGTGA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bcar1 ( XM_023267416.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023123184.1 Amphiprion ocellaris BCAR1, Cas family scaffolding protein (bcar1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAm75640
Clone ID Related Accession (Same CDS sequence)	XM_023267416.1
Accession Version	XM_023267416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	breast cancer anti-estrogen resistance protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019371841.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTCC CTAACGTGTT GGCCAAGGCC TTGTATGACA ACGTGGCTGA GTCTCCAGAT 
GAACTGTCCT TCCGCAAAGG TGACATCATG ACAGTGTTGG AGCGTGACAC ACAGGGATTG 
GATGGCTGGT GGCTCTGCTC ACTCCACGGA CGCCAAGGCA TCGTCCCTGG CAATCGTCTG 
AAGATTCTGG TGGGCATGTA TGACAGTAAG CAGCAGCAGC AGGCCACTCC TCCCACACCA 
GATCTACTTG TTTCCTGTTC TGCCTCCCAG GTTCAGCGAC CCCTCCCCAT TCAAAGCACC 
TACGCCAAAC CAGCACCTGC TTTGTCATCT GCTGCCATCG CTACGTCCTC TGGGTTTGCC 
ACCAAGCCCC TCCCCTCAAC ACAGTACACG TCCATGCACC CAGCCTACTC CACCCCCAGC 
CCTGCCCAGC CCAACCCTGA CTCTGTCTAC ATGATGCCGC CCTCCCATGG TTCAAAACCA 
AGCCCCCAAA GTCTATACCA GGTTCCCTCT GGTCCAAGTG GACCCTCTGC ACAGGCACAG 
CCAGGACCCC CAAGCAAGGC TCCACCTCTA GGACAAAGAC AGTACCAGCT CCCTGGACAG 
GATATTTATC AGGTGCCCCC CTGTGGAGGC CCAGGACCAG GCCATGGAGC AGTTCCATCT 
GGGGGTACAG GAGCTGTCCA GGATGTGTAC CAGGTGCCCC CCACTCTGGA TAAGAGGAAC 
TGGGAGAGCA GCAACAAGCC TCTTGGCAAG GTGGTTGTTC CTACCCGTGT TGGACAGGTG 
TATGTATACG ACACAGTAAA ACCAGACCGG GACGAGTACG ACATCCCACC AAGACATCAG 
CCCCCAACCC AGCAGGACAT ATATGACGTG CCTCCCACCC GCCAGCAGTA CAGTACACAG 
GTGTATGATA CTCCTCCCAT GGTGGTGAAG GATCCCTCTA GTGGTCAAGA TATATATGAC 
ATCCCAGCAA GCACAGACAA GAACCCACAA CAGACTGTCT ATGACTTCCC ACCATCTGTC 
AGTAAAGACA TACCTGTTTC TCAACCAATA AGAGAGGAGA CCTATGATGT GCCACCCCAC 
TTTGCCAAAA TAAAGTCCCA AGTCGCCATC ACTCCTGGCC AGTACCTGCA GAACAACATC 
AATGACGATG ACGAAGAGCC GCCGATTCCA GAGGATGTTT ACGACGTCCC ACCCCCTATC 
CTGACTGACA AGCATTATCA CAGAGACAGG AATGGATTCA GCCAGGCCCC CCAGGAGATC 
TATGACATCC CAGCCATTCT GCATCCTGGA GGGTACCCCA CCAAGGATGT ATATGACTTC 
CCCCGAGAAC GAGAGGAACA AGGAGGAGAG AGGGGAGGCC AGTATGTCTA TGATGTCCCA 
CCACAGGTTG TTCGTGATGC CCAGTCAACC AGTGAAGAGC TGACAATGTC TTTCAAGCGG 
CTGTCTGCCT CAAGCACAGG AAGCACACGG AGCAACCACT CTACTTCATC ATTAGACATT 
GTCCCTGTCC GTGACACCTC CTCACTTCCT GCTCCAAGCC CCATTCCAGG GAAACCACTC 
ATCTTGGACC TGGATCAGGC CATGGAGCGT CTGTCTCGCC TCCAGCAAGC TGTTGAGTCC 
AGTGTTTCCT TCATGATGTC ATTCATTACA GGCAATTGGA GAAACCCTGC TCACCTGGAG 
GGGAATCTTC CGGCTATTCA TCAGGCTGCT GACCGGGTGC GAGTCACTGT TCGAGACTTT 
CTAGAATTTG CTCGGGGTGC TGTGGCCAAT GCTACCCAGG TTACAGACCG CTCACTACAG 
ACCAAACTAG GCCGTCAGGT GGGAAAGATG GAGGAGGTCT TCCAGAGTTT GATTCGACAC 
AGTCAGAGCT TAGACACAAT TTCTTGGTCA CACACAGCAC TCACAACACC ACCACCAGGA 
GGGGATGACT TAGATCTACT AATTATGACA GCGCGAGGCA TCCCCGATGA CGCCAAGCAG 
CTTGCCTCCT TTCTCCATGG TAATGCCTCA CTGCTCTTCA AACGGACGAC CCGGCAACAG 
CAGCAGCTGC CTCTTCCTCC GATCCCTGGG GAGATCAGTG GTCACATGAC AGGGTCAGGA 
AGTGGAAGCT TTCAGGGAGG GGAGAAGGTG AATATTCAGT CACGGCCCCT CCCCTCTCCA 
CCAAAATTTA CAGCTGCAGA GGACGAGGAA GGTGTGGACA GGCCGTATGA GACCACAGAG 
GAAGGCTGGA TGGAAGACTA TGACTACGTA CATCTACAGG GAAAGGAAGA GTTTGAGAAG 
AACCAAAGAC AACTGCTGGA AAAAGGCAAT ATTATCAGAC ACAAGAAGAC ACAACTGGAA 
CAGCAACAGA TTAAACAGTT TGAGAGGCTA GAGCAGGAAG TCAGCCGGCC AATCAACAAT 
GACATGACGG GCTGGGTTCC ATCCCCACAT CACCCTCTAG CCAACCAGCT GGCCCAGAAC 
GGCTCTGGAG GCTCCTCCTC CTCCAAGCTG TGCCATGGCG ACCAGCAGCT CCTCCTCTTC 
TATCAGGAGC AATGTGAACA GAATGTCACC ACAGTGACCA ACGCCATTGA TGCTTTCTTT 
ACGGCCATCA ACTCTAACCA GCCACCAAAG ATCTTTGTGG CACACAGCAA ATTTGTCATT 
CTCAGTGCAC ATAAGCTGGT GTTCATTGGC GACACACTGT CACGTCAGGC CAAGACTCCT 
GAGGTGAGGG CCCGGGTTGC CCAAAGCAGC AACACCCTCT GTGAAAAACT CAAAGACATT 
GTGATCAGTA CAAAGACAGC AGCGCTTCAA TATCCTTCGC CTGGAGCAGC CAGAGAGATG 
ACTGAGCGGG TAAAGGAGCT GGCAGGATAT ACACAGCAGT TCAGGATGGT GCTGGGACAA 
CTGCTGGTGA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023123184.1
CDS	423..3317
Translation	MSVPNVLAKALYDNVAESPDELSFRKGDIMTVLERDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDSKQQQQATPPTPDLLVSCSASQVQRPLPIQSTYAKPAPALSSAAIATSSGFATKPLPSTQYTSMHPAYSTPSPAQPNPDSVYMMPPSHGSKPSPQSLYQVPSGPSGPSAQAQPGPPSKAPPLGQRQYQLPGQDIYQVPPCGGPGPGHGAVPSGGTGAVQDVYQVPPTLDKRNWESSNKPLGKVVVPTRVGQVYVYDTVKPDRDEYDIPPRHQPPTQQDIYDVPPTRQQYSTQVYDTPPMVVKDPSSGQDIYDIPASTDKNPQQTVYDFPPSVSKDIPVSQPIREETYDVPPHFAKIKSQVAITPGQYLQNNINDDDEEPPIPEDVYDVPPPILTDKHYHRDRNGFSQAPQEIYDIPAILHPGGYPTKDVYDFPREREEQGGERGGQYVYDVPPQVVRDAQSTSEELTMSFKRLSASSTGSTRSNHSTSSLDIVPVRDTSSLPAPSPIPGKPLILDLDQAMERLSRLQQAVESSVSFMMSFITGNWRNPAHLEGNLPAIHQAADRVRVTVRDFLEFARGAVANATQVTDRSLQTKLGRQVGKMEEVFQSLIRHSQSLDTISWSHTALTTPPPGGDDLDLLIMTARGIPDDAKQLASFLHGNASLLFKRTTRQQQQLPLPPIPGEISGHMTGSGSGSFQGGEKVNIQSRPLPSPPKFTAAEDEEGVDRPYETTEEGWMEDYDYVHLQGKEEFEKNQRQLLEKGNIIRHKKTQLEQQQIKQFERLEQEVSRPINNDMTGWVPSPHHPLANQLAQNGSGGSSSSKLCHGDQQLLLFYQEQCEQNVTTVTNAIDAFFTAINSNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKTPEVRARVAQSSNTLCEKLKDIVISTKTAALQYPSPGAAREMTERVKELAGYTQQFRMVLGQLLVM

Target ORF information:

RefSeq Version	XM_023267416.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris BCAR1, Cas family scaffolding protein (bcar1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023267416.1

ORF Insert Sequence:

ATGTCTGTCC CTAACGTGTT GGCCAAGGCC TTGTATGACA ACGTGGCTGA GTCTCCAGAT 
GAACTGTCCT TCCGCAAAGG TGACATCATG ACAGTGTTGG AGCGTGACAC ACAGGGATTG 
GATGGCTGGT GGCTCTGCTC ACTCCACGGA CGCCAAGGCA TCGTCCCTGG CAATCGTCTG 
AAGATTCTGG TGGGCATGTA TGACAGTAAG CAGCAGCAGC AGGCCACTCC TCCCACACCA 
GATCTACTTG TTTCCTGTTC TGCCTCCCAG GTTCAGCGAC CCCTCCCCAT TCAAAGCACC 
TACGCCAAAC CAGCACCTGC TTTGTCATCT GCTGCCATCG CTACGTCCTC TGGGTTTGCC 
ACCAAGCCCC TCCCCTCAAC ACAGTACACG TCCATGCACC CAGCCTACTC CACCCCCAGC 
CCTGCCCAGC CCAACCCTGA CTCTGTCTAC ATGATGCCGC CCTCCCATGG TTCAAAACCA 
AGCCCCCAAA GTCTATACCA GGTTCCCTCT GGTCCAAGTG GACCCTCTGC ACAGGCACAG 
CCAGGACCCC CAAGCAAGGC TCCACCTCTA GGACAAAGAC AGTACCAGCT CCCTGGACAG 
GATATTTATC AGGTGCCCCC CTGTGGAGGC CCAGGACCAG GCCATGGAGC AGTTCCATCT 
GGGGGTACAG GAGCTGTCCA GGATGTGTAC CAGGTGCCCC CCACTCTGGA TAAGAGGAAC 
TGGGAGAGCA GCAACAAGCC TCTTGGCAAG GTGGTTGTTC CTACCCGTGT TGGACAGGTG 
TATGTATACG ACACAGTAAA ACCAGACCGG GACGAGTACG ACATCCCACC AAGACATCAG 
CCCCCAACCC AGCAGGACAT ATATGACGTG CCTCCCACCC GCCAGCAGTA CAGTACACAG 
GTGTATGATA CTCCTCCCAT GGTGGTGAAG GATCCCTCTA GTGGTCAAGA TATATATGAC 
ATCCCAGCAA GCACAGACAA GAACCCACAA CAGACTGTCT ATGACTTCCC ACCATCTGTC 
AGTAAAGACA TACCTGTTTC TCAACCAATA AGAGAGGAGA CCTATGATGT GCCACCCCAC 
TTTGCCAAAA TAAAGTCCCA AGTCGCCATC ACTCCTGGCC AGTACCTGCA GAACAACATC 
AATGACGATG ACGAAGAGCC GCCGATTCCA GAGGATGTTT ACGACGTCCC ACCCCCTATC 
CTGACTGACA AGCATTATCA CAGAGACAGG AATGGATTCA GCCAGGCCCC CCAGGAGATC 
TATGACATCC CAGCCATTCT GCATCCTGGA GGGTACCCCA CCAAGGATGT ATATGACTTC 
CCCCGAGAAC GAGAGGAACA AGGAGGAGAG AGGGGAGGCC AGTATGTCTA TGATGTCCCA 
CCACAGGTTG TTCGTGATGC CCAGTCAACC AGTGAAGAGC TGACAATGTC TTTCAAGCGG 
CTGTCTGCCT CAAGCACAGG AAGCACACGG AGCAACCACT CTACTTCATC ATTAGACATT 
GTCCCTGTCC GTGACACCTC CTCACTTCCT GCTCCAAGCC CCATTCCAGG GAAACCACTC 
ATCTTGGACC TGGATCAGGC CATGGAGCGT CTGTCTCGCC TCCAGCAAGC TGTTGAGTCC 
AGTGTTTCCT TCATGATGTC ATTCATTACA GGCAATTGGA GAAACCCTGC TCACCTGGAG 
GGGAATCTTC CGGCTATTCA TCAGGCTGCT GACCGGGTGC GAGTCACTGT TCGAGACTTT 
CTAGAATTTG CTCGGGGTGC TGTGGCCAAT GCTACCCAGG TTACAGACCG CTCACTACAG 
ACCAAACTAG GCCGTCAGGT GGGAAAGATG GAGGAGGTCT TCCAGAGTTT GATTCGACAC 
AGTCAGAGCT TAGACACAAT TTCTTGGTCA CACACAGCAC TCACAACACC ACCACCAGGA 
GGGGATGACT TAGATCTACT AATTATGACA GCGCGAGGCA TCCCCGATGA CGCCAAGCAG 
CTTGCCTCCT TTCTCCATGG TAATGCCTCA CTGCTCTTCA AACGGACGAC CCGGCAACAG 
CAGCAGCTGC CTCTTCCTCC GATCCCTGGG GAGATCAGTG GTCACATGAC AGGGTCAGGA 
AGTGGAAGCT TTCAGGGAGG GGAGAAGGTG AATATTCAGT CACGGCCCCT CCCCTCTCCA 
CCAAAATTTA CAGCTGCAGA GGACGAGGAA GGTGTGGACA GGCCGTATGA GACCACAGAG 
GAAGGCTGGA TGGAAGACTA TGACTACGTA CATCTACAGG GAAAGGAAGA GTTTGAGAAG 
AACCAAAGAC AACTGCTGGA AAAAGGCAAT ATTATCAGAC ACAAGAAGAC ACAACTGGAA 
CAGCAACAGA TTAAACAGTT TGAGAGGCTA GAGCAGGAAG TCAGCCGGCC AATCAACAAT 
GACATGACGG GCTGGGTTCC ATCCCCACAT CACCCTCTAG CCAACCAGCT GGCCCAGAAC 
GGCTCTGGAG GCTCCTCCTC CTCCAAGCTG TGCCATGGCG ACCAGCAGCT CCTCCTCTTC 
TATCAGGAGC AATGTGAACA GAATGTCACC ACAGTGACCA ACGCCATTGA TGCTTTCTTT 
ACGGCCATCA ACTCTAACCA GCCACCAAAG ATCTTTGTGG CACACAGCAA ATTTGTCATT 
CTCAGTGCAC ATAAGCTGGT GTTCATTGGC GACACACTGT CACGTCAGGC CAAGACTCCT 
GAGGTGAGGG CCCGGGTTGC CCAAAGCAGC AACACCCTCT GTGAAAAACT CAAAGACATT 
GTGATCAGTA CAAAGACAGC AGCGCTTCAA TATCCTTCGC CTGGAGCAGC CAGAGAGATG 
ACTGAGCGGG TAAAGGAGCT GGCAGGATAT ACACAGCAGT TCAGGATGGT GCTGGGACAA 
CTGCTGGTGA TGTAG