arhgef1 cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	arhgef1
Entrez Gene ID	111564210
Full Name	Rho guanine nucleotide exchange factor 1
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023263656.1	XP_023119424.1	rho guanine nucleotide exchange factor 1 isoform X4
XM_023263657.1	XP_023119425.1	rho guanine nucleotide exchange factor 1 isoform X5
XM_023263652.1	XP_023119420.1	rho guanine nucleotide exchange factor 1 isoform X1
XM_023263653.1	XP_023119421.1	rho guanine nucleotide exchange factor 1 isoform X2
XM_023263658.1	XP_023119426.1	rho guanine nucleotide exchange factor 1 isoform X6
XM_023263659.1	XP_023119427.1	rho guanine nucleotide exchange factor 1 isoform X7
XM_023263655.1	XP_023119423.1	rho guanine nucleotide exchange factor 1 isoform X3
XM_023263660.1	XP_023119428.1	rho guanine nucleotide exchange factor 1 isoform X8

Pathways from BioSystems
Gene Ontology

The following arhgef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAm103089	XM_023263659.1 Latest version!	Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAm103090	XM_023263660.1 Latest version!	Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAm103083	XM_023263652.1 Latest version!	Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAm103084	XM_023263653.1 Latest version!	Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAm103085	XM_023263655.1 Latest version!	Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAm103086	XM_023263656.1 Latest version!	Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAm103087	XM_023263657.1 Latest version!	Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAm103088	XM_023263658.1 Latest version!	Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arhgef1 ( XM_023263659.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023119427.1 Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OAm103089
Clone ID Related Accession (Same CDS sequence)	XM_023263659.1
Accession Version	XM_023263659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	rho guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376149.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATCA TCGGGGCCGA GGATGAAGAT TTTGAGAATG ATCTGAATGA TGTGATGGAT 
GACCAGTCCC CGCACTTCAA CAGCATAGAG GTGTTGAAGG AGCGGCCGAC CCACCTGCTG 
GTCTTCATGC AACATGTCAT TCTGCAGTTT GACCCTGCTC CCTTGCTGTG TTACCTCCAT 
GCTGACCTCT TCAGGAGCCT CAGTGCCAAA GAGACCAAGA AACAGTTTGT GGAGTTCTAC 
AATACCTTTT TGGACAAGGG TGCAATTCTC AGAGTGACAA TACCACCTTC AATATCATAT 
GAATTGGACC GGACCCGTCC AGATCTGTTC TCAGAAGAGA CCCAAAGGCG TTTTGCTCAG 
GAGGTTCAGA ACCTGCAGAC TGTTGAAGTG GCAAAACAGT TGGAGGATTT CAGGCAGAAG 
AGAAGGATGG GTATGACGCC CAATGAAGCT GAACTAATTG ATGTGGAGAG TCACTATCCC 
ACCGACCGCG TCCCAATGGA AATGAAGGAA AAGTCTGTGG CTGAGAACCT GCTGGAGAAG 
ATGTCTGAGA CACAACCTAC GATTGTCTCG GACGATGAAA AATGCCAATC CATCTTTACT 
GCAGTGGCCT TCTACATGAA GCACCTTGGA GTTAAAACCA GGGCAGTTGA CAGTAAGAAG 
TCTAGAGGAG GCTTCTTCAG GAGTAAACTG GTAAAGAATA AGAAGGATGA ATCTACAAAG 
CCCAAGCCCA GAGGGGTGTT TCCTGGCATC CCAAGCTGGA TTGGAGGCAA CACTGAGGTC 
AAACCTGAAA CGGAGGCTAA AGCGGAAAAG GAGAAAGTGG CTCTGGATCG TAAAGGCCCT 
GCTCCTGGCA GAGGCTCCTT GACCGACCCT GCAGTCCCTG CCCTCCCTAG CAGAAAGGCT 
GGTTCCAGTG GAAGCCCTGC CTCACTGCCT GGACTAGAGA TTAGTGATGG CTCGGGCAAC 
AACATCAGCA CTACCAATAG CCCAGAGTCT TTACACACTG ATGGTCTTTC AAGCAATCGT 
TTAGAGCCCC CAGTACTGTC AGACGGGGGT GATGTTTCCC CCGTCGGGAT TTCAGGTGGG 
CTAATCATTG GGGAGCCCAG CTCACCTTGT GACACACCCA CAGACGAGAC TGTGGAGAAA 
GACAGTGTTG ATGGAACAGT TTTAGTTCCT GAAGGACCTG GCCACTCCCC CACCTGTCCC 
TCACCACAGC TGGAGGAGAT GGAGCCACGT CTCTTGGAGT TTGAGCAGGA CCCACCCAAC 
TGGAGAGAGC TGGCCTCTCC TGAAGCCTTG TCCAGCCTCA GCAAAAAGGA GACTAAAAGG 
CAGGAAGTTA TTAATGAGCT GATTGCTACA GAGCATGCCC ATGTGAGAAT GCTAAGTGTC 
CTTCAGATGG TCTTCTCCAA ACCATTGGAG AGACAAGAAC TCCTGGCCGC TACTGAGCTG 
GCTGCCATCT TCCCCAGCCT CGATGAGATC ATTGAAATGC ACTATAACTT CTACGAGAAC 
CTGAAAAAGC TGCGGCTGGA TGACAGCTTC ATAGTTAAAT CCATCAGCAC CACAGTGCTC 
AACAGATTCG GTGGTACAGA GGGTGAGTGG TTTCAGAAGC TGACAGCCCG GTTCTGCAGT 
CACCAGTCAT GGGCCCTGGA TCAGATTAAG GGCAGACAAA GGAAAGACCC ACGCTTCAAT 
TCCTTTATAC AGGAGGCAGA GAGTAAGCCC CAGTGCCGCA GGCTGCAGCT CAAGGACATT 
ATTCCTATAG AGATGCAGAG ACTGACCAAG TATCCATTGC TGCTGGAGAA TATTGCAAAG 
AACACAGAGA ACCCAACAGA GAAGGAGAGC ATCCAGCAGA GTGCAGAGTG CTGCAGAAAA 
ATCCTCAACC ATGTCAATGA GGAGGTCAAA GTAATGGAGA ACCTGTTGAC TGTGAAGGAC 
TACCAGCGTA GATTGGACAC ATCAGGTCTT AAACCCAGTA ACGAACTTTA TACAGAATAC 
AAGAACATTG ACTTGACTCA GAAGAAGATG CTTTATGAAG GCCCACTGAC CTGGAAAGTC 
ACCAAAGAGA AGGCTATTGA GGTGCAGTGT GTGTTGCTTG GTGACCTGCT AGTGCTCCTA 
CAGAGGCAAG ATGACAAGAT GGTTCTCAAA TGCCAGAGCA AAAGTACCAT CGCTGTGCAG 
GAGGGCAAGC AGATGCTGAG CCCCATTATT AAGCTGGACT CAGTTTTCCT CCGTGAGGTG 
GCCACAGATC GAAAGGCTTT CTACGTGATA TTTACCTGGG ACAGTGGTGC TCAAATCTAT 
GAACTCGTGG CTCAGTCTGT TGGAGAAATG AAAACTTGGA CTGAGGTGAT AAAGTTAGCA 
GTAGATGATC TGAAAAAGAG TGGTGCACCT ATGAGGCTGC CACTGCCTCC AGGAGGTGGA 
GGAGCTCCCT TCAGTCCCTC TACGCTAAAT GCCCCTGTGA GCCTGACTGA GAATGGAAGT 
TTGAAAAGCG GCGCTGATCG AGACAAGTAC GGTTTGAAGT CTACGGATCC CCGGCACAGG 
CTAATCGATT TCCTGTCAGA TAAAGGCTTT GACTTGATAG GCCACTCCAA CAGCATTCAG 
GAGAAGGTGG CCAGTCGTGC TTTGGATGAC GTCATGTCAC TGAAAAGACT GTTAATAGGC 
AGCATCAGTC TATCAGACGA CTCAAAACCT GATGAAGAAA ATGAAGAGGA ACAATCAGAG 
AGGCCCGATC AAGAAAGGGA TTGCTGTCAG TCAACAGAAG AAAATCCGAC CAGAGACGGA 
GGGGCTGAAG TGGTAAACGT CAACACATGT AAAAAAGAAG GTGAAGAGAT GAAAGGAGAT 
GAAGAAAGGA GCATCAGTGC TCCCCTGGTT TTATCCCAGG AGAGGCTGGA GGAAGTGTAC 
AGGAGGCTGC AGAGCCTGGA GGAACAGCTA GAGAGACTAC AGAATGTAGA AGAGGAGCAC 
CACAGGCTGC AGGAGGCTCT TTCTGAGTTC TCACTGGAAC GGGGTAATTT CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023119427.1
CDS	107..3103
Translation	MSIIGAEDEDFENDLNDVMDDQSPHFNSIEVLKERPTHLLVFMQHVILQFDPAPLLCYLHADLFRSLSAKETKKQFVEFYNTFLDKGAILRVTIPPSISYELDRTRPDLFSEETQRRFAQEVQNLQTVEVAKQLEDFRQKRRMGMTPNEAELIDVESHYPTDRVPMEMKEKSVAENLLEKMSETQPTIVSDDEKCQSIFTAVAFYMKHLGVKTRAVDSKKSRGGFFRSKLVKNKKDESTKPKPRGVFPGIPSWIGGNTEVKPETEAKAEKEKVALDRKGPAPGRGSLTDPAVPALPSRKAGSSGSPASLPGLEISDGSGNNISTTNSPESLHTDGLSSNRLEPPVLSDGGDVSPVGISGGLIIGEPSSPCDTPTDETVEKDSVDGTVLVPEGPGHSPTCPSPQLEEMEPRLLEFEQDPPNWRELASPEALSSLSKKETKRQEVINELIATEHAHVRMLSVLQMVFSKPLERQELLAATELAAIFPSLDEIIEMHYNFYENLKKLRLDDSFIVKSISTTVLNRFGGTEGEWFQKLTARFCSHQSWALDQIKGRQRKDPRFNSFIQEAESKPQCRRLQLKDIIPIEMQRLTKYPLLLENIAKNTENPTEKESIQQSAECCRKILNHVNEEVKVMENLLTVKDYQRRLDTSGLKPSNELYTEYKNIDLTQKKMLYEGPLTWKVTKEKAIEVQCVLLGDLLVLLQRQDDKMVLKCQSKSTIAVQEGKQMLSPIIKLDSVFLREVATDRKAFYVIFTWDSGAQIYELVAQSVGEMKTWTEVIKLAVDDLKKSGAPMRLPLPPGGGGAPFSPSTLNAPVSLTENGSLKSGADRDKYGLKSTDPRHRLIDFLSDKGFDLIGHSNSIQEKVASRALDDVMSLKRLLIGSISLSDDSKPDEENEEEQSERPDQERDCCQSTEENPTRDGGAEVVNVNTCKKEGEEMKGDEERSISAPLVLSQERLEEVYRRLQSLEEQLERLQNVEEEHHRLQEALSEFSLERGNFQ

Target ORF information:

RefSeq Version	XM_023263659.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023263659.1

ORF Insert Sequence:

ATGAGCATCA TCGGGGCCGA GGATGAAGAT TTTGAGAATG ATCTGAATGA TGTGATGGAT 
GACCAGTCCC CGCACTTCAA CAGCATAGAG GTGTTGAAGG AGCGGCCGAC CCACCTGCTG 
GTCTTCATGC AACATGTCAT TCTGCAGTTT GACCCTGCTC CCTTGCTGTG TTACCTCCAT 
GCTGACCTCT TCAGGAGCCT CAGTGCCAAA GAGACCAAGA AACAGTTTGT GGAGTTCTAC 
AATACCTTTT TGGACAAGGG TGCAATTCTC AGAGTGACAA TACCACCTTC AATATCATAT 
GAATTGGACC GGACCCGTCC AGATCTGTTC TCAGAAGAGA CCCAAAGGCG TTTTGCTCAG 
GAGGTTCAGA ACCTGCAGAC TGTTGAAGTG GCAAAACAGT TGGAGGATTT CAGGCAGAAG 
AGAAGGATGG GTATGACGCC CAATGAAGCT GAACTAATTG ATGTGGAGAG TCACTATCCC 
ACCGACCGCG TCCCAATGGA AATGAAGGAA AAGTCTGTGG CTGAGAACCT GCTGGAGAAG 
ATGTCTGAGA CACAACCTAC GATTGTCTCG GACGATGAAA AATGCCAATC CATCTTTACT 
GCAGTGGCCT TCTACATGAA GCACCTTGGA GTTAAAACCA GGGCAGTTGA CAGTAAGAAG 
TCTAGAGGAG GCTTCTTCAG GAGTAAACTG GTAAAGAATA AGAAGGATGA ATCTACAAAG 
CCCAAGCCCA GAGGGGTGTT TCCTGGCATC CCAAGCTGGA TTGGAGGCAA CACTGAGGTC 
AAACCTGAAA CGGAGGCTAA AGCGGAAAAG GAGAAAGTGG CTCTGGATCG TAAAGGCCCT 
GCTCCTGGCA GAGGCTCCTT GACCGACCCT GCAGTCCCTG CCCTCCCTAG CAGAAAGGCT 
GGTTCCAGTG GAAGCCCTGC CTCACTGCCT GGACTAGAGA TTAGTGATGG CTCGGGCAAC 
AACATCAGCA CTACCAATAG CCCAGAGTCT TTACACACTG ATGGTCTTTC AAGCAATCGT 
TTAGAGCCCC CAGTACTGTC AGACGGGGGT GATGTTTCCC CCGTCGGGAT TTCAGGTGGG 
CTAATCATTG GGGAGCCCAG CTCACCTTGT GACACACCCA CAGACGAGAC TGTGGAGAAA 
GACAGTGTTG ATGGAACAGT TTTAGTTCCT GAAGGACCTG GCCACTCCCC CACCTGTCCC 
TCACCACAGC TGGAGGAGAT GGAGCCACGT CTCTTGGAGT TTGAGCAGGA CCCACCCAAC 
TGGAGAGAGC TGGCCTCTCC TGAAGCCTTG TCCAGCCTCA GCAAAAAGGA GACTAAAAGG 
CAGGAAGTTA TTAATGAGCT GATTGCTACA GAGCATGCCC ATGTGAGAAT GCTAAGTGTC 
CTTCAGATGG TCTTCTCCAA ACCATTGGAG AGACAAGAAC TCCTGGCCGC TACTGAGCTG 
GCTGCCATCT TCCCCAGCCT CGATGAGATC ATTGAAATGC ACTATAACTT CTACGAGAAC 
CTGAAAAAGC TGCGGCTGGA TGACAGCTTC ATAGTTAAAT CCATCAGCAC CACAGTGCTC 
AACAGATTCG GTGGTACAGA GGGTGAGTGG TTTCAGAAGC TGACAGCCCG GTTCTGCAGT 
CACCAGTCAT GGGCCCTGGA TCAGATTAAG GGCAGACAAA GGAAAGACCC ACGCTTCAAT 
TCCTTTATAC AGGAGGCAGA GAGTAAGCCC CAGTGCCGCA GGCTGCAGCT CAAGGACATT 
ATTCCTATAG AGATGCAGAG ACTGACCAAG TATCCATTGC TGCTGGAGAA TATTGCAAAG 
AACACAGAGA ACCCAACAGA GAAGGAGAGC ATCCAGCAGA GTGCAGAGTG CTGCAGAAAA 
ATCCTCAACC ATGTCAATGA GGAGGTCAAA GTAATGGAGA ACCTGTTGAC TGTGAAGGAC 
TACCAGCGTA GATTGGACAC ATCAGGTCTT AAACCCAGTA ACGAACTTTA TACAGAATAC 
AAGAACATTG ACTTGACTCA GAAGAAGATG CTTTATGAAG GCCCACTGAC CTGGAAAGTC 
ACCAAAGAGA AGGCTATTGA GGTGCAGTGT GTGTTGCTTG GTGACCTGCT AGTGCTCCTA 
CAGAGGCAAG ATGACAAGAT GGTTCTCAAA TGCCAGAGCA AAAGTACCAT CGCTGTGCAG 
GAGGGCAAGC AGATGCTGAG CCCCATTATT AAGCTGGACT CAGTTTTCCT CCGTGAGGTG 
GCCACAGATC GAAAGGCTTT CTACGTGATA TTTACCTGGG ACAGTGGTGC TCAAATCTAT 
GAACTCGTGG CTCAGTCTGT TGGAGAAATG AAAACTTGGA CTGAGGTGAT AAAGTTAGCA 
GTAGATGATC TGAAAAAGAG TGGTGCACCT ATGAGGCTGC CACTGCCTCC AGGAGGTGGA 
GGAGCTCCCT TCAGTCCCTC TACGCTAAAT GCCCCTGTGA GCCTGACTGA GAATGGAAGT 
TTGAAAAGCG GCGCTGATCG AGACAAGTAC GGTTTGAAGT CTACGGATCC CCGGCACAGG 
CTAATCGATT TCCTGTCAGA TAAAGGCTTT GACTTGATAG GCCACTCCAA CAGCATTCAG 
GAGAAGGTGG CCAGTCGTGC TTTGGATGAC GTCATGTCAC TGAAAAGACT GTTAATAGGC 
AGCATCAGTC TATCAGACGA CTCAAAACCT GATGAAGAAA ATGAAGAGGA ACAATCAGAG 
AGGCCCGATC AAGAAAGGGA TTGCTGTCAG TCAACAGAAG AAAATCCGAC CAGAGACGGA 
GGGGCTGAAG TGGTAAACGT CAACACATGT AAAAAAGAAG GTGAAGAGAT GAAAGGAGAT 
GAAGAAAGGA GCATCAGTGC TCCCCTGGTT TTATCCCAGG AGAGGCTGGA GGAAGTGTAC 
AGGAGGCTGC AGAGCCTGGA GGAACAGCTA GAGAGACTAC AGAATGTAGA AGAGGAGCAC 
CACAGGCTGC AGGAGGCTCT TTCTGAGTTC TCACTGGAAC GGGGTAATTT CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_023263660.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023119428.1 Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OAm103090
Clone ID Related Accession (Same CDS sequence)	XM_023263660.1
Accession Version	XM_023263660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	rho guanine nucleotide exchange factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376149.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACTTA AAAGAAAGAG ACCTACGATT GTCTCGGACG ATGAAAAATG CCAATCCATC 
TTTACTGCAG TGGCCTTCTA CATGAAGCAC CTTGGAGTTA AAACCAGGGC AGTTGACAGT 
AAGAAGTCTA GAGGAGGCTT CTTCAGGAGT AAACTGGTAA AGAATAAGAA GGATGAATCT 
ACAAAGCCCA AGCCCAGAGG GGTGTTTCCT GGCATCCCAA GCTGGATTGG AGGCAACACT 
GAGGTCAAAC CTGAAACGGA GGCTAAAGCG GAAAAGGAGA AAGTGGCTCT GGATCGTAAA 
GGCCCTGCTC CTGGCAGAGG CTCCTTGACC GACCCTGCAG TCCCTGCCCT CCCTAGCAGA 
AAGGCTGGTT CCAGTGGAAG CCCTGCCTCA CTGCCTGGAC TAGAGATTAG TGATGGCTCG 
GGCAACAACA TCAGCACTAC CAATAGCCCA GAGTCTTTAC ACACTGATGG TCTTTCAAGC 
AATCGTTTAG AGCCCCCAGT ACTGTCAGAC GGGGGTGATG TTTCCCCCGT CGGGATTTCA 
GGTGGGCTAA TCATTGGGGA GCCCAGCTCA CCTTGTGACA CACCCACAGA CGAGACTGTG 
GAGAAAGACA GACGGAAGAC AAGTAGGAAA GTGGGGCGCA GTGAGAGTGC ACGTGTGGAC 
CGGCACTCGT ATCGGCGCCG TGGCTCTTCT CGGGCTAAAC AGTCTCGCTC CCGTAGTGAT 
GTGGACCTTC AGCCACCTTC TACCACGACA ACACCAACTG CCCTGCTCAC CCCCCAGCAA 
CTCCATCCTG TTGATGGAAC AGTTTTAGTT CCTGAAGGAC CTGGCCACTC CCCCACCTGT 
CCCTCACCAC AGCTGGAGGA GATGGAGCCA CGTCTCTTGG AGTTTGAGCA GGACCCACCC 
AACTGGAGAG AGCTGGCCTC TCCTGAAGCC TTGTCCAGCC TCAGCAAAAA GGAGACTAAA 
AGGCAGGAAG TTATTAATGA GCTGATTGCT ACAGAGCATG CCCATGTGAG AATGCTAAGT 
GTCCTTCAGA TGGTCTTCTC CAAACCATTG GAGAGACAAG AACTCCTGGC CGCTACTGAG 
CTGGCTGCCA TCTTCCCCAG CCTCGATGAG ATCATTGAAA TGCACTATAA CTTCTACGAG 
AACCTGAAAA AGCTGCGGCT GGATGACAGC TTCATAGTTA AATCCATCAG CACCACAGTG 
CTCAACAGAT TCGGTGGTAC AGAGGGTGAG TGGTTTCAGA AGCTGACAGC CCGGTTCTGC 
AGTCACCAGT CATGGGCCCT GGATCAGATT AAGGGCAGAC AAAGGAAAGA CCCACGCTTC 
AATTCCTTTA TACAGGAGGC AGAGAGTAAG CCCCAGTGCC GCAGGCTGCA GCTCAAGGAC 
ATTATTCCTA TAGAGATGCA GAGACTGACC AAGTATCCAT TGCTGCTGGA GAATATTGCA 
AAGAACACAG AGAACCCAAC AGAGAAGGAG AGCATCCAGC AGAGTGCAGA GTGCTGCAGA 
AAAATCCTCA ACCATGTCAA TGAGGAGGTC AAAGTAATGG AGAACCTGTT GACTGTGAAG 
GACTACCAGC GTAGATTGGA CACATCAGGT CTTAAACCCA GTAACGAACT TTATACAGAA 
TACAAGAACA TTGACTTGAC TCAGAAGAAG ATGCTTTATG AAGGCCCACT GACCTGGAAA 
GTCACCAAAG AGAAGGCTAT TGAGGTGCAG TGTGTGTTGC TTGGTGACCT GCTAGTGCTC 
CTACAGAGGC AAGATGACAA GATGGTTCTC AAATGCCAGA GCAAAAGTAC CATCGCTGTG 
CAGGAGGGCA AGCAGATGCT GAGCCCCATT ATTAAGCTGG ACTCAGTTTT CCTCCGTGAG 
GTGGCCACAG ATCGAAAGGC TTTCTACGTG ATATTTACCT GGGACAGTGG TGCTCAAATC 
TATGAACTCG TGGCTCAGTC TGTTGGAGAA ATGAAAACTT GGACTGAGGT GATAAAGTTA 
GCAGTAGATG ATCTGAAAAA GAGTGGTGCA CCTATGAGGC TGCCACTGCC TCCAGGAGGT 
GGAGGAGCTC CCTTCAGTCC CTCTACGCTA AATGCCCCTG TGAGCCTGAC TGAGAATGGA 
AGTTTGAAAA GCGGCGCTGA TCGAGACAAG TACGGTTTGA AGTCTACGGA TCCCCGGCAC 
AGGCTAATCG ATTTCCTGTC AGATAAAGGC TTTGACTTGA TAGGCCACTC CAACAGCATT 
CAGGAGAAGG TGGCCAGTCG TGCTTTGGAT GACGTCATGT CACTGAAAAG ACTGTTAATA 
GGCAGCATCA GTCTATCAGA CGACTCAAAA CCTGATGAAG AAAATGAAGA GGAACAATCA 
GAGAGGCCCG ATCAAGAAAG GGATTGCTGT CAGTCAACAG AAGAAAATCC GACCAGAGAC 
GGAGGGGCTG AAGTGGTAAA CGTCAACACA TGTAAAAAAG AAGGTGAAGA GATGAAAGGA 
GATGAAGAAA GGAGCATCAG TGCTCCCCTG GTTTTATCCC AGGAGAGGCT GGAGGAAGTG 
TACAGGAGGC TGCAGAGCCT GGAGGAACAG CTAGAGAGAC TACAGAATGT AGAAGAGGAG 
CACCACAGGC TGCAGGAGGC TCTTTCTGAG TTCTCACTGG AACGGGGTAA TTTCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023119428.1
CDS	150..2789
Translation	MLLKRKRPTIVSDDEKCQSIFTAVAFYMKHLGVKTRAVDSKKSRGGFFRSKLVKNKKDESTKPKPRGVFPGIPSWIGGNTEVKPETEAKAEKEKVALDRKGPAPGRGSLTDPAVPALPSRKAGSSGSPASLPGLEISDGSGNNISTTNSPESLHTDGLSSNRLEPPVLSDGGDVSPVGISGGLIIGEPSSPCDTPTDETVEKDRRKTSRKVGRSESARVDRHSYRRRGSSRAKQSRSRSDVDLQPPSTTTTPTALLTPQQLHPVDGTVLVPEGPGHSPTCPSPQLEEMEPRLLEFEQDPPNWRELASPEALSSLSKKETKRQEVINELIATEHAHVRMLSVLQMVFSKPLERQELLAATELAAIFPSLDEIIEMHYNFYENLKKLRLDDSFIVKSISTTVLNRFGGTEGEWFQKLTARFCSHQSWALDQIKGRQRKDPRFNSFIQEAESKPQCRRLQLKDIIPIEMQRLTKYPLLLENIAKNTENPTEKESIQQSAECCRKILNHVNEEVKVMENLLTVKDYQRRLDTSGLKPSNELYTEYKNIDLTQKKMLYEGPLTWKVTKEKAIEVQCVLLGDLLVLLQRQDDKMVLKCQSKSTIAVQEGKQMLSPIIKLDSVFLREVATDRKAFYVIFTWDSGAQIYELVAQSVGEMKTWTEVIKLAVDDLKKSGAPMRLPLPPGGGGAPFSPSTLNAPVSLTENGSLKSGADRDKYGLKSTDPRHRLIDFLSDKGFDLIGHSNSIQEKVASRALDDVMSLKRLLIGSISLSDDSKPDEENEEEQSERPDQERDCCQSTEENPTRDGGAEVVNVNTCKKEGEEMKGDEERSISAPLVLSQERLEEVYRRLQSLEEQLERLQNVEEEHHRLQEALSEFSLERGNFQ

Target ORF information:

RefSeq Version	XM_023263660.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023263660.1

ORF Insert Sequence:

ATGTTACTTA AAAGAAAGAG ACCTACGATT GTCTCGGACG ATGAAAAATG CCAATCCATC 
TTTACTGCAG TGGCCTTCTA CATGAAGCAC CTTGGAGTTA AAACCAGGGC AGTTGACAGT 
AAGAAGTCTA GAGGAGGCTT CTTCAGGAGT AAACTGGTAA AGAATAAGAA GGATGAATCT 
ACAAAGCCCA AGCCCAGAGG GGTGTTTCCT GGCATCCCAA GCTGGATTGG AGGCAACACT 
GAGGTCAAAC CTGAAACGGA GGCTAAAGCG GAAAAGGAGA AAGTGGCTCT GGATCGTAAA 
GGCCCTGCTC CTGGCAGAGG CTCCTTGACC GACCCTGCAG TCCCTGCCCT CCCTAGCAGA 
AAGGCTGGTT CCAGTGGAAG CCCTGCCTCA CTGCCTGGAC TAGAGATTAG TGATGGCTCG 
GGCAACAACA TCAGCACTAC CAATAGCCCA GAGTCTTTAC ACACTGATGG TCTTTCAAGC 
AATCGTTTAG AGCCCCCAGT ACTGTCAGAC GGGGGTGATG TTTCCCCCGT CGGGATTTCA 
GGTGGGCTAA TCATTGGGGA GCCCAGCTCA CCTTGTGACA CACCCACAGA CGAGACTGTG 
GAGAAAGACA GACGGAAGAC AAGTAGGAAA GTGGGGCGCA GTGAGAGTGC ACGTGTGGAC 
CGGCACTCGT ATCGGCGCCG TGGCTCTTCT CGGGCTAAAC AGTCTCGCTC CCGTAGTGAT 
GTGGACCTTC AGCCACCTTC TACCACGACA ACACCAACTG CCCTGCTCAC CCCCCAGCAA 
CTCCATCCTG TTGATGGAAC AGTTTTAGTT CCTGAAGGAC CTGGCCACTC CCCCACCTGT 
CCCTCACCAC AGCTGGAGGA GATGGAGCCA CGTCTCTTGG AGTTTGAGCA GGACCCACCC 
AACTGGAGAG AGCTGGCCTC TCCTGAAGCC TTGTCCAGCC TCAGCAAAAA GGAGACTAAA 
AGGCAGGAAG TTATTAATGA GCTGATTGCT ACAGAGCATG CCCATGTGAG AATGCTAAGT 
GTCCTTCAGA TGGTCTTCTC CAAACCATTG GAGAGACAAG AACTCCTGGC CGCTACTGAG 
CTGGCTGCCA TCTTCCCCAG CCTCGATGAG ATCATTGAAA TGCACTATAA CTTCTACGAG 
AACCTGAAAA AGCTGCGGCT GGATGACAGC TTCATAGTTA AATCCATCAG CACCACAGTG 
CTCAACAGAT TCGGTGGTAC AGAGGGTGAG TGGTTTCAGA AGCTGACAGC CCGGTTCTGC 
AGTCACCAGT CATGGGCCCT GGATCAGATT AAGGGCAGAC AAAGGAAAGA CCCACGCTTC 
AATTCCTTTA TACAGGAGGC AGAGAGTAAG CCCCAGTGCC GCAGGCTGCA GCTCAAGGAC 
ATTATTCCTA TAGAGATGCA GAGACTGACC AAGTATCCAT TGCTGCTGGA GAATATTGCA 
AAGAACACAG AGAACCCAAC AGAGAAGGAG AGCATCCAGC AGAGTGCAGA GTGCTGCAGA 
AAAATCCTCA ACCATGTCAA TGAGGAGGTC AAAGTAATGG AGAACCTGTT GACTGTGAAG 
GACTACCAGC GTAGATTGGA CACATCAGGT CTTAAACCCA GTAACGAACT TTATACAGAA 
TACAAGAACA TTGACTTGAC TCAGAAGAAG ATGCTTTATG AAGGCCCACT GACCTGGAAA 
GTCACCAAAG AGAAGGCTAT TGAGGTGCAG TGTGTGTTGC TTGGTGACCT GCTAGTGCTC 
CTACAGAGGC AAGATGACAA GATGGTTCTC AAATGCCAGA GCAAAAGTAC CATCGCTGTG 
CAGGAGGGCA AGCAGATGCT GAGCCCCATT ATTAAGCTGG ACTCAGTTTT CCTCCGTGAG 
GTGGCCACAG ATCGAAAGGC TTTCTACGTG ATATTTACCT GGGACAGTGG TGCTCAAATC 
TATGAACTCG TGGCTCAGTC TGTTGGAGAA ATGAAAACTT GGACTGAGGT GATAAAGTTA 
GCAGTAGATG ATCTGAAAAA GAGTGGTGCA CCTATGAGGC TGCCACTGCC TCCAGGAGGT 
GGAGGAGCTC CCTTCAGTCC CTCTACGCTA AATGCCCCTG TGAGCCTGAC TGAGAATGGA 
AGTTTGAAAA GCGGCGCTGA TCGAGACAAG TACGGTTTGA AGTCTACGGA TCCCCGGCAC 
AGGCTAATCG ATTTCCTGTC AGATAAAGGC TTTGACTTGA TAGGCCACTC CAACAGCATT 
CAGGAGAAGG TGGCCAGTCG TGCTTTGGAT GACGTCATGT CACTGAAAAG ACTGTTAATA 
GGCAGCATCA GTCTATCAGA CGACTCAAAA CCTGATGAAG AAAATGAAGA GGAACAATCA 
GAGAGGCCCG ATCAAGAAAG GGATTGCTGT CAGTCAACAG AAGAAAATCC GACCAGAGAC 
GGAGGGGCTG AAGTGGTAAA CGTCAACACA TGTAAAAAAG AAGGTGAAGA GATGAAAGGA 
GATGAAGAAA GGAGCATCAG TGCTCCCCTG GTTTTATCCC AGGAGAGGCT GGAGGAAGTG 
TACAGGAGGC TGCAGAGCCT GGAGGAACAG CTAGAGAGAC TACAGAATGT AGAAGAGGAG 
CACCACAGGC TGCAGGAGGC TCTTTCTGAG TTCTCACTGG AACGGGGTAA TTTCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_023263652.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023119420.1 Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAm103083
Clone ID Related Accession (Same CDS sequence)	XM_023263652.1
Accession Version	XM_023263652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	rho guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376149.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATCA TCGGGGCCGA GGATGAAGAT TTTGAGAATG ATCTGAATGA TGTGATGGAT 
GACCAGTCCC CGCACTTCAA CAGCATAGAG GTGTTGAAGG AGCGGCCGAC CCACCTGCTG 
GTCTTCATGC AACATGTCAT TCTGCAGTTT GACCCTGCTC CCTTGCTGTG TTACCTCCAT 
GCTGACCTCT TCAGGAGCCT CAGTGCCAAA GAGACCAAGA AACAGTTTGT GGAGTTCTAC 
AATACCTTTT TGGACAAGGG TGCAATTCTC AGAGTGACAA TACCACCTTC AATATCATAT 
GAATTGGACC GGACCCGTCC AGATCTGTTC TCAGAAGAGA CCCAAAGGCG TTTTGCTCAG 
GAGGTTCAGA ACCTGCAGAC TGTTGAAGTG GCAAAACAGT TGGAGGATTT CAGGCAGAAG 
AGAAGGATGG GTATGACGCC CAATGAAGCT GAACTAATTG ATGTGGAGAG TCACTATCCC 
ACCGACCGCG TCCCAATGGA AATGAAGGAA AAGTCTGTGG CTGAGAACCT GCTGGAGAAG 
ATGTCTGAGA CACAACCTAC GATTGTCTCG GACGATGAAA AATGCCAATC CATCTTTACT 
GCAGTGGCCT TCTACATGAA GCACCTTGGA GTTAAAACCA GGGCAGTTGA CAGTAAGAAG 
TCTAGAGGAG GCTTCTTCAG GAGTAAACTG GTAAAGAATA AGAAGGATGA ATCTACAAAG 
CCCAAGCCCA GAGGGGTGTT TCCTGGCATC CCAAGCTGGA TTGGAGGCAA CACTGAGGTC 
AAACCTGAAA CGGAGGCTAA AGCGGAAAAG GAGAAAGTGG CTCTGGATCG TAAAGGCCCT 
GCTCCTGGCA GAGGCTCCTT GACCGACCCT GCAGTCCCTG CCCTCCCTAG CAGAAAGGCT 
GGTTCCAGTG GAAGCCCTGC CTCACTGCCT GGACTAGAGA TTAGTGATGG CTCGGGCAAC 
AACATCAGCA CTACCAATAG CCCAGAGTCT TTACACACTG ATGGTCTTTC AAGCAATCGT 
TTAGAGCCCC CAGTACTGTC AGACGGGGGT GATGTTTCCC CCGTCGGGAT TTCAGGTGGG 
CTAATCATTG GGGAGCCCAG CTCACCTTGT GACACACCCA CAGACGAGAC TGTGGAGAAA 
GACAGACGGA AGACAAGTAG GAAAGTGGGG CGCAGTGAGA GTGCACGTGT GGACCGGCAC 
TCGTATCGGC GCCGTGGCTC TTCTCGGGCT AAACAGTCTC GCTCCCGTAG TGATGTGGAC 
CTTCAGCCAC CTTCTACCAC GACAACACCA ACTGCCCTGC TCACCCCCCA GCAACTCCAT 
CCTGTTGATG GAACAGTTTT AGTTCCTGAA GGACCTGGCC ACTCCCCCAC CTGTCCCTCA 
CCACAGCTGG AGGAGATGGA GCCACGTCTC TTGGAGTTTG AGCAGGACCC ACCCAACTGG 
AGAGAGCTGG CCTCTCCTGA AGCCTTGTCC AGCCTCAGCA AAAAGGAGAC TAAAAGGCAG 
GAAGTTATTA ATGAGCTGAT TGCTACAGAG CATGCCCATG TGAGAATGCT AAGTGTCCTT 
CAGATGGTCT TCTCCAAACC ATTGGAGAGA CAAGAACTCC TGGCCGCTAC TGAGCTGGCT 
GCCATCTTCC CCAGCCTCGA TGAGATCATT GAAATGCACT ATAACTTCTA CGAGAACCTG 
AAAAAGCTGC GGCTGGATGA CAGCTTCATA GTTAAATCCA TCAGCACCAC AGTGCTCAAC 
AGATTCGGTG GTACAGAGGG TGAGTGGTTT CAGAAGCTGA CAGCCCGGTT CTGCAGTCAC 
CAGTCATGGG CCCTGGATCA GATTAAGGGC AGACAAAGGA AAGACCCACG CTTCAATTCC 
TTTATACAGG AGGCAGAGAG TAAGCCCCAG TGCCGCAGGC TGCAGCTCAA GGACATTATT 
CCTATAGAGA TGCAGAGACT GACCAAGTAT CCATTGCTGC TGGAGAATAT TGCAAAGAAC 
ACAGAGAACC CAACAGAGAA GGAGAGCATC CAGCAGAGTG CAGAGTGCTG CAGAAAAATC 
CTCAACCATG TCAATGAGGA GGTCAAAGTA ATGGAGAACC TGTTGACTGT GAAGGACTAC 
CAGCGTAGAT TGGACACATC AGGTCTTAAA CCCAGTAACG AACTTTATAC AGAATACAAG 
AACATTGACT TGACTCAGAA GAAGATGCTT TATGAAGGCC CACTGACCTG GAAAGTCACC 
AAAGAGAAGG CTATTGAGGT GCAGTGTGTG TTGCTTGGTG ACCTGCTAGT GCTCCTACAG 
AGGCAAGATG ACAAGATGGT TCTCAAATGC CAGAGCAAAA GTACCATCGC TGTGCAGGAG 
GGCAAGCAGA TGCTGAGCCC CATTATTAAG CTGGACTCAG TTTTCCTCCG TGAGGTGGCC 
ACAGATCGAA AGGCTTTCTA CGTGATATTT ACCTGGGACA GTGGTGCTCA AATCTATGAA 
CTCGTGGCTC AGTCTGTTGG AGAAATGAAA ACTTGGACTG AGGTGATAAA GTTAGCAGTA 
GATGATCTGA AAAAGAGTGG TGCACCTATG AGGCTGCCAC TGCCTCCAGG AGGTGGAGGA 
GCTCCCTTCA GTCCCTCTAC GCTAAATGCC CCTGTGAGCC TGACTGAGAA TGGAAGTTTG 
AAAAGCGGCG CTGATCGAGA CAAGTACGGT TTGAAGTCTA CGGATCCCCG GCACAGGCTA 
ATCGATTTCC TGTCAGATAA AGGCTTTGAC TTGATAGGCC ACTCCAACAG CATTCAGGAG 
AAGGTGGCCA GTCGTGCTTT GGATGACGTC ATGTCACTGA AAAGACTGTT AATAGGCAGC 
ATCAGTCTAT CAGACGACTC AAAACCTGAT GAAGAAAATG AAGAGGAACA ATCAGAGAGG 
CCCGATCAAG AAAGGGATTG CTGTCAGTCA ACAGAAGAAA ATCCGACCAG AGACGGAGGG 
GCTGAAGTGG TAAACGTCAA CACATGTAAA AAAGAAGGTG AAGAGATGAA AGGAGATGAA 
GAAAGGAGCA TCAGTGCTCC CCTGGTTTTA TCCCAGGAGA GGCTGGAGGA AGTGTACAGG 
AGGCTGCAGA GCCTGGAGGA ACAGCTAGAG AGACTACAGA ATGTAGAAGA GGAGCACCAC 
AGGCTGCAGG AGGCTCTTTC TGAGTTCTCA CTGGAACGGG GTAATTTCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023119420.1
CDS	107..3280
Translation	MSIIGAEDEDFENDLNDVMDDQSPHFNSIEVLKERPTHLLVFMQHVILQFDPAPLLCYLHADLFRSLSAKETKKQFVEFYNTFLDKGAILRVTIPPSISYELDRTRPDLFSEETQRRFAQEVQNLQTVEVAKQLEDFRQKRRMGMTPNEAELIDVESHYPTDRVPMEMKEKSVAENLLEKMSETQPTIVSDDEKCQSIFTAVAFYMKHLGVKTRAVDSKKSRGGFFRSKLVKNKKDESTKPKPRGVFPGIPSWIGGNTEVKPETEAKAEKEKVALDRKGPAPGRGSLTDPAVPALPSRKAGSSGSPASLPGLEISDGSGNNISTTNSPESLHTDGLSSNRLEPPVLSDGGDVSPVGISGGLIIGEPSSPCDTPTDETVEKDRRKTSRKVGRSESARVDRHSYRRRGSSRAKQSRSRSDVDLQPPSTTTTPTALLTPQQLHPVDGTVLVPEGPGHSPTCPSPQLEEMEPRLLEFEQDPPNWRELASPEALSSLSKKETKRQEVINELIATEHAHVRMLSVLQMVFSKPLERQELLAATELAAIFPSLDEIIEMHYNFYENLKKLRLDDSFIVKSISTTVLNRFGGTEGEWFQKLTARFCSHQSWALDQIKGRQRKDPRFNSFIQEAESKPQCRRLQLKDIIPIEMQRLTKYPLLLENIAKNTENPTEKESIQQSAECCRKILNHVNEEVKVMENLLTVKDYQRRLDTSGLKPSNELYTEYKNIDLTQKKMLYEGPLTWKVTKEKAIEVQCVLLGDLLVLLQRQDDKMVLKCQSKSTIAVQEGKQMLSPIIKLDSVFLREVATDRKAFYVIFTWDSGAQIYELVAQSVGEMKTWTEVIKLAVDDLKKSGAPMRLPLPPGGGGAPFSPSTLNAPVSLTENGSLKSGADRDKYGLKSTDPRHRLIDFLSDKGFDLIGHSNSIQEKVASRALDDVMSLKRLLIGSISLSDDSKPDEENEEEQSERPDQERDCCQSTEENPTRDGGAEVVNVNTCKKEGEEMKGDEERSISAPLVLSQERLEEVYRRLQSLEEQLERLQNVEEEHHRLQEALSEFSLERGNFQ

Target ORF information:

RefSeq Version	XM_023263652.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023263652.1

ORF Insert Sequence:

ATGAGCATCA TCGGGGCCGA GGATGAAGAT TTTGAGAATG ATCTGAATGA TGTGATGGAT 
GACCAGTCCC CGCACTTCAA CAGCATAGAG GTGTTGAAGG AGCGGCCGAC CCACCTGCTG 
GTCTTCATGC AACATGTCAT TCTGCAGTTT GACCCTGCTC CCTTGCTGTG TTACCTCCAT 
GCTGACCTCT TCAGGAGCCT CAGTGCCAAA GAGACCAAGA AACAGTTTGT GGAGTTCTAC 
AATACCTTTT TGGACAAGGG TGCAATTCTC AGAGTGACAA TACCACCTTC AATATCATAT 
GAATTGGACC GGACCCGTCC AGATCTGTTC TCAGAAGAGA CCCAAAGGCG TTTTGCTCAG 
GAGGTTCAGA ACCTGCAGAC TGTTGAAGTG GCAAAACAGT TGGAGGATTT CAGGCAGAAG 
AGAAGGATGG GTATGACGCC CAATGAAGCT GAACTAATTG ATGTGGAGAG TCACTATCCC 
ACCGACCGCG TCCCAATGGA AATGAAGGAA AAGTCTGTGG CTGAGAACCT GCTGGAGAAG 
ATGTCTGAGA CACAACCTAC GATTGTCTCG GACGATGAAA AATGCCAATC CATCTTTACT 
GCAGTGGCCT TCTACATGAA GCACCTTGGA GTTAAAACCA GGGCAGTTGA CAGTAAGAAG 
TCTAGAGGAG GCTTCTTCAG GAGTAAACTG GTAAAGAATA AGAAGGATGA ATCTACAAAG 
CCCAAGCCCA GAGGGGTGTT TCCTGGCATC CCAAGCTGGA TTGGAGGCAA CACTGAGGTC 
AAACCTGAAA CGGAGGCTAA AGCGGAAAAG GAGAAAGTGG CTCTGGATCG TAAAGGCCCT 
GCTCCTGGCA GAGGCTCCTT GACCGACCCT GCAGTCCCTG CCCTCCCTAG CAGAAAGGCT 
GGTTCCAGTG GAAGCCCTGC CTCACTGCCT GGACTAGAGA TTAGTGATGG CTCGGGCAAC 
AACATCAGCA CTACCAATAG CCCAGAGTCT TTACACACTG ATGGTCTTTC AAGCAATCGT 
TTAGAGCCCC CAGTACTGTC AGACGGGGGT GATGTTTCCC CCGTCGGGAT TTCAGGTGGG 
CTAATCATTG GGGAGCCCAG CTCACCTTGT GACACACCCA CAGACGAGAC TGTGGAGAAA 
GACAGACGGA AGACAAGTAG GAAAGTGGGG CGCAGTGAGA GTGCACGTGT GGACCGGCAC 
TCGTATCGGC GCCGTGGCTC TTCTCGGGCT AAACAGTCTC GCTCCCGTAG TGATGTGGAC 
CTTCAGCCAC CTTCTACCAC GACAACACCA ACTGCCCTGC TCACCCCCCA GCAACTCCAT 
CCTGTTGATG GAACAGTTTT AGTTCCTGAA GGACCTGGCC ACTCCCCCAC CTGTCCCTCA 
CCACAGCTGG AGGAGATGGA GCCACGTCTC TTGGAGTTTG AGCAGGACCC ACCCAACTGG 
AGAGAGCTGG CCTCTCCTGA AGCCTTGTCC AGCCTCAGCA AAAAGGAGAC TAAAAGGCAG 
GAAGTTATTA ATGAGCTGAT TGCTACAGAG CATGCCCATG TGAGAATGCT AAGTGTCCTT 
CAGATGGTCT TCTCCAAACC ATTGGAGAGA CAAGAACTCC TGGCCGCTAC TGAGCTGGCT 
GCCATCTTCC CCAGCCTCGA TGAGATCATT GAAATGCACT ATAACTTCTA CGAGAACCTG 
AAAAAGCTGC GGCTGGATGA CAGCTTCATA GTTAAATCCA TCAGCACCAC AGTGCTCAAC 
AGATTCGGTG GTACAGAGGG TGAGTGGTTT CAGAAGCTGA CAGCCCGGTT CTGCAGTCAC 
CAGTCATGGG CCCTGGATCA GATTAAGGGC AGACAAAGGA AAGACCCACG CTTCAATTCC 
TTTATACAGG AGGCAGAGAG TAAGCCCCAG TGCCGCAGGC TGCAGCTCAA GGACATTATT 
CCTATAGAGA TGCAGAGACT GACCAAGTAT CCATTGCTGC TGGAGAATAT TGCAAAGAAC 
ACAGAGAACC CAACAGAGAA GGAGAGCATC CAGCAGAGTG CAGAGTGCTG CAGAAAAATC 
CTCAACCATG TCAATGAGGA GGTCAAAGTA ATGGAGAACC TGTTGACTGT GAAGGACTAC 
CAGCGTAGAT TGGACACATC AGGTCTTAAA CCCAGTAACG AACTTTATAC AGAATACAAG 
AACATTGACT TGACTCAGAA GAAGATGCTT TATGAAGGCC CACTGACCTG GAAAGTCACC 
AAAGAGAAGG CTATTGAGGT GCAGTGTGTG TTGCTTGGTG ACCTGCTAGT GCTCCTACAG 
AGGCAAGATG ACAAGATGGT TCTCAAATGC CAGAGCAAAA GTACCATCGC TGTGCAGGAG 
GGCAAGCAGA TGCTGAGCCC CATTATTAAG CTGGACTCAG TTTTCCTCCG TGAGGTGGCC 
ACAGATCGAA AGGCTTTCTA CGTGATATTT ACCTGGGACA GTGGTGCTCA AATCTATGAA 
CTCGTGGCTC AGTCTGTTGG AGAAATGAAA ACTTGGACTG AGGTGATAAA GTTAGCAGTA 
GATGATCTGA AAAAGAGTGG TGCACCTATG AGGCTGCCAC TGCCTCCAGG AGGTGGAGGA 
GCTCCCTTCA GTCCCTCTAC GCTAAATGCC CCTGTGAGCC TGACTGAGAA TGGAAGTTTG 
AAAAGCGGCG CTGATCGAGA CAAGTACGGT TTGAAGTCTA CGGATCCCCG GCACAGGCTA 
ATCGATTTCC TGTCAGATAA AGGCTTTGAC TTGATAGGCC ACTCCAACAG CATTCAGGAG 
AAGGTGGCCA GTCGTGCTTT GGATGACGTC ATGTCACTGA AAAGACTGTT AATAGGCAGC 
ATCAGTCTAT CAGACGACTC AAAACCTGAT GAAGAAAATG AAGAGGAACA ATCAGAGAGG 
CCCGATCAAG AAAGGGATTG CTGTCAGTCA ACAGAAGAAA ATCCGACCAG AGACGGAGGG 
GCTGAAGTGG TAAACGTCAA CACATGTAAA AAAGAAGGTG AAGAGATGAA AGGAGATGAA 
GAAAGGAGCA TCAGTGCTCC CCTGGTTTTA TCCCAGGAGA GGCTGGAGGA AGTGTACAGG 
AGGCTGCAGA GCCTGGAGGA ACAGCTAGAG AGACTACAGA ATGTAGAAGA GGAGCACCAC 
AGGCTGCAGG AGGCTCTTTC TGAGTTCTCA CTGGAACGGG GTAATTTCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_023263653.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023119421.1 Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAm103084
Clone ID Related Accession (Same CDS sequence)	XM_023263653.1
Accession Version	XM_023263653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	rho guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376149.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATCA TCGGGGCCGA GGATGAAGAT TTTGAGAATG ATCTGAATGA TGTGATGGAT 
GACCAGTCCC CGCACTTCAA CAGCATAGAG GTGTTGAAGG AGCGGCCGAC CCACCTGCTG 
GTCTTCATGC AACATGTCAT TCTGCAGTTT GACCCTGCTC CCTTGCTGTG TTACCTCCAT 
GCTGACCTCT TCAGGAGCCT CAGTGCCAAA GAGACCAAGA AACAGTTTGT GGAGTTCTAC 
AATACCTTTT TGGACAAGGG TGCAATTCTC AGAGTGACAA TACCACCTTC AATATCATAT 
GAATTGGACC GGACCCGTCC AGATCTGTTC TCAGAAGAGA CCCAAAGGCG TTTTGCTCAG 
GAGGTTCAGA ACCTGCAGAC TGTTGAAGTG GCAAAACAGT TGGAGGATTT CAGGCAGAAG 
AGAAGGATGG GTATGACGCC CAATGAAGCT GAACTAATTG ATGTGGAGAG TCACTATCCC 
ACCGACCGCG TCCCAATGGA AATGAAGGAA AAGTCTGTGG CTGAGAACCT GCTGGAGAAG 
ATGTCTGAGA CACAACCTAC GATTGTCTCG GACGATGAAA AATGCCAATC CATCTTTACT 
GCAGTGGCCT TCTACATGAA GCACCTTGGA GTTAAAACCA GGGCAGTTGA CAGTAAGAAG 
TCTAGAGGAG GCTTCTTCAG GAGTAAACTG GTAAAGAATA AGAAGGATGA ATCTACAAAG 
CCCAAGCCCA GAGGGGTGTT TCCTGGCATC CCAAGCTGGA TTGGAGGCAA CACTGAGGTC 
AAACCTGAAA CGGAGGCTAA AGCGGAAAAG GAGAAAGTGG CTCTGGATCG TAAAGGCCCT 
GCTCCTGGCA GAGGCTCCTT GACCGACCCT GCAGTCCCTG CCCTCCCTAG CAGAAAGGCT 
GGTTCCAGTG GAAGCCCTGC CTCACTGCCT GGACTAGAGA TTAGTGATGG CTCGGGCAAC 
AACATCAGCA CTACCAATAG CCCAGAGTCT TTACACACTG ATGGTCTTTC AAGCAATCGT 
TTAGAGCCCC CAGTACTGTC AGACGGGGGT GATGTTTCCC CCGTCGGGAT TTCAGGTGGG 
CTAATCATTG GGGAGCCCAG CTCACCTTGT GACACACCCA CAGACGAGAC TGTGGAGAAA 
GACAGACGGA AGACAAGGAA AGTGGGGCGC AGTGAGAGTG CACGTGTGGA CCGGCACTCG 
TATCGGCGCC GTGGCTCTTC TCGGGCTAAA CAGTCTCGCT CCCGTAGTGA TGTGGACCTT 
CAGCCACCTT CTACCACGAC AACACCAACT GCCCTGCTCA CCCCCCAGCA ACTCCATCCT 
GTTGATGGAA CAGTTTTAGT TCCTGAAGGA CCTGGCCACT CCCCCACCTG TCCCTCACCA 
CAGCTGGAGG AGATGGAGCC ACGTCTCTTG GAGTTTGAGC AGGACCCACC CAACTGGAGA 
GAGCTGGCCT CTCCTGAAGC CTTGTCCAGC CTCAGCAAAA AGGAGACTAA AAGGCAGGAA 
GTTATTAATG AGCTGATTGC TACAGAGCAT GCCCATGTGA GAATGCTAAG TGTCCTTCAG 
ATGGTCTTCT CCAAACCATT GGAGAGACAA GAACTCCTGG CCGCTACTGA GCTGGCTGCC 
ATCTTCCCCA GCCTCGATGA GATCATTGAA ATGCACTATA ACTTCTACGA GAACCTGAAA 
AAGCTGCGGC TGGATGACAG CTTCATAGTT AAATCCATCA GCACCACAGT GCTCAACAGA 
TTCGGTGGTA CAGAGGGTGA GTGGTTTCAG AAGCTGACAG CCCGGTTCTG CAGTCACCAG 
TCATGGGCCC TGGATCAGAT TAAGGGCAGA CAAAGGAAAG ACCCACGCTT CAATTCCTTT 
ATACAGGAGG CAGAGAGTAA GCCCCAGTGC CGCAGGCTGC AGCTCAAGGA CATTATTCCT 
ATAGAGATGC AGAGACTGAC CAAGTATCCA TTGCTGCTGG AGAATATTGC AAAGAACACA 
GAGAACCCAA CAGAGAAGGA GAGCATCCAG CAGAGTGCAG AGTGCTGCAG AAAAATCCTC 
AACCATGTCA ATGAGGAGGT CAAAGTAATG GAGAACCTGT TGACTGTGAA GGACTACCAG 
CGTAGATTGG ACACATCAGG TCTTAAACCC AGTAACGAAC TTTATACAGA ATACAAGAAC 
ATTGACTTGA CTCAGAAGAA GATGCTTTAT GAAGGCCCAC TGACCTGGAA AGTCACCAAA 
GAGAAGGCTA TTGAGGTGCA GTGTGTGTTG CTTGGTGACC TGCTAGTGCT CCTACAGAGG 
CAAGATGACA AGATGGTTCT CAAATGCCAG AGCAAAAGTA CCATCGCTGT GCAGGAGGGC 
AAGCAGATGC TGAGCCCCAT TATTAAGCTG GACTCAGTTT TCCTCCGTGA GGTGGCCACA 
GATCGAAAGG CTTTCTACGT GATATTTACC TGGGACAGTG GTGCTCAAAT CTATGAACTC 
GTGGCTCAGT CTGTTGGAGA AATGAAAACT TGGACTGAGG TGATAAAGTT AGCAGTAGAT 
GATCTGAAAA AGAGTGGTGC ACCTATGAGG CTGCCACTGC CTCCAGGAGG TGGAGGAGCT 
CCCTTCAGTC CCTCTACGCT AAATGCCCCT GTGAGCCTGA CTGAGAATGG AAGTTTGAAA 
AGCGGCGCTG ATCGAGACAA GTACGGTTTG AAGTCTACGG ATCCCCGGCA CAGGCTAATC 
GATTTCCTGT CAGATAAAGG CTTTGACTTG ATAGGCCACT CCAACAGCAT TCAGGAGAAG 
GTGGCCAGTC GTGCTTTGGA TGACGTCATG TCACTGAAAA GACTGTTAAT AGGCAGCATC 
AGTCTATCAG ACGACTCAAA ACCTGATGAA GAAAATGAAG AGGAACAATC AGAGAGGCCC 
GATCAAGAAA GGGATTGCTG TCAGTCAACA GAAGAAAATC CGACCAGAGA CGGAGGGGCT 
GAAGTGGTAA ACGTCAACAC ATGTAAAAAA GAAGGTGAAG AGATGAAAGG AGATGAAGAA 
AGGAGCATCA GTGCTCCCCT GGTTTTATCC CAGGAGAGGC TGGAGGAAGT GTACAGGAGG 
CTGCAGAGCC TGGAGGAACA GCTAGAGAGA CTACAGAATG TAGAAGAGGA GCACCACAGG 
CTGCAGGAGG CTCTTTCTGA GTTCTCACTG GAACGGGGTA ATTTCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023119421.1
CDS	107..3277
Translation	MSIIGAEDEDFENDLNDVMDDQSPHFNSIEVLKERPTHLLVFMQHVILQFDPAPLLCYLHADLFRSLSAKETKKQFVEFYNTFLDKGAILRVTIPPSISYELDRTRPDLFSEETQRRFAQEVQNLQTVEVAKQLEDFRQKRRMGMTPNEAELIDVESHYPTDRVPMEMKEKSVAENLLEKMSETQPTIVSDDEKCQSIFTAVAFYMKHLGVKTRAVDSKKSRGGFFRSKLVKNKKDESTKPKPRGVFPGIPSWIGGNTEVKPETEAKAEKEKVALDRKGPAPGRGSLTDPAVPALPSRKAGSSGSPASLPGLEISDGSGNNISTTNSPESLHTDGLSSNRLEPPVLSDGGDVSPVGISGGLIIGEPSSPCDTPTDETVEKDRRKTRKVGRSESARVDRHSYRRRGSSRAKQSRSRSDVDLQPPSTTTTPTALLTPQQLHPVDGTVLVPEGPGHSPTCPSPQLEEMEPRLLEFEQDPPNWRELASPEALSSLSKKETKRQEVINELIATEHAHVRMLSVLQMVFSKPLERQELLAATELAAIFPSLDEIIEMHYNFYENLKKLRLDDSFIVKSISTTVLNRFGGTEGEWFQKLTARFCSHQSWALDQIKGRQRKDPRFNSFIQEAESKPQCRRLQLKDIIPIEMQRLTKYPLLLENIAKNTENPTEKESIQQSAECCRKILNHVNEEVKVMENLLTVKDYQRRLDTSGLKPSNELYTEYKNIDLTQKKMLYEGPLTWKVTKEKAIEVQCVLLGDLLVLLQRQDDKMVLKCQSKSTIAVQEGKQMLSPIIKLDSVFLREVATDRKAFYVIFTWDSGAQIYELVAQSVGEMKTWTEVIKLAVDDLKKSGAPMRLPLPPGGGGAPFSPSTLNAPVSLTENGSLKSGADRDKYGLKSTDPRHRLIDFLSDKGFDLIGHSNSIQEKVASRALDDVMSLKRLLIGSISLSDDSKPDEENEEEQSERPDQERDCCQSTEENPTRDGGAEVVNVNTCKKEGEEMKGDEERSISAPLVLSQERLEEVYRRLQSLEEQLERLQNVEEEHHRLQEALSEFSLERGNFQ

Target ORF information:

RefSeq Version	XM_023263653.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023263653.1

ORF Insert Sequence:

ATGAGCATCA TCGGGGCCGA GGATGAAGAT TTTGAGAATG ATCTGAATGA TGTGATGGAT 
GACCAGTCCC CGCACTTCAA CAGCATAGAG GTGTTGAAGG AGCGGCCGAC CCACCTGCTG 
GTCTTCATGC AACATGTCAT TCTGCAGTTT GACCCTGCTC CCTTGCTGTG TTACCTCCAT 
GCTGACCTCT TCAGGAGCCT CAGTGCCAAA GAGACCAAGA AACAGTTTGT GGAGTTCTAC 
AATACCTTTT TGGACAAGGG TGCAATTCTC AGAGTGACAA TACCACCTTC AATATCATAT 
GAATTGGACC GGACCCGTCC AGATCTGTTC TCAGAAGAGA CCCAAAGGCG TTTTGCTCAG 
GAGGTTCAGA ACCTGCAGAC TGTTGAAGTG GCAAAACAGT TGGAGGATTT CAGGCAGAAG 
AGAAGGATGG GTATGACGCC CAATGAAGCT GAACTAATTG ATGTGGAGAG TCACTATCCC 
ACCGACCGCG TCCCAATGGA AATGAAGGAA AAGTCTGTGG CTGAGAACCT GCTGGAGAAG 
ATGTCTGAGA CACAACCTAC GATTGTCTCG GACGATGAAA AATGCCAATC CATCTTTACT 
GCAGTGGCCT TCTACATGAA GCACCTTGGA GTTAAAACCA GGGCAGTTGA CAGTAAGAAG 
TCTAGAGGAG GCTTCTTCAG GAGTAAACTG GTAAAGAATA AGAAGGATGA ATCTACAAAG 
CCCAAGCCCA GAGGGGTGTT TCCTGGCATC CCAAGCTGGA TTGGAGGCAA CACTGAGGTC 
AAACCTGAAA CGGAGGCTAA AGCGGAAAAG GAGAAAGTGG CTCTGGATCG TAAAGGCCCT 
GCTCCTGGCA GAGGCTCCTT GACCGACCCT GCAGTCCCTG CCCTCCCTAG CAGAAAGGCT 
GGTTCCAGTG GAAGCCCTGC CTCACTGCCT GGACTAGAGA TTAGTGATGG CTCGGGCAAC 
AACATCAGCA CTACCAATAG CCCAGAGTCT TTACACACTG ATGGTCTTTC AAGCAATCGT 
TTAGAGCCCC CAGTACTGTC AGACGGGGGT GATGTTTCCC CCGTCGGGAT TTCAGGTGGG 
CTAATCATTG GGGAGCCCAG CTCACCTTGT GACACACCCA CAGACGAGAC TGTGGAGAAA 
GACAGACGGA AGACAAGGAA AGTGGGGCGC AGTGAGAGTG CACGTGTGGA CCGGCACTCG 
TATCGGCGCC GTGGCTCTTC TCGGGCTAAA CAGTCTCGCT CCCGTAGTGA TGTGGACCTT 
CAGCCACCTT CTACCACGAC AACACCAACT GCCCTGCTCA CCCCCCAGCA ACTCCATCCT 
GTTGATGGAA CAGTTTTAGT TCCTGAAGGA CCTGGCCACT CCCCCACCTG TCCCTCACCA 
CAGCTGGAGG AGATGGAGCC ACGTCTCTTG GAGTTTGAGC AGGACCCACC CAACTGGAGA 
GAGCTGGCCT CTCCTGAAGC CTTGTCCAGC CTCAGCAAAA AGGAGACTAA AAGGCAGGAA 
GTTATTAATG AGCTGATTGC TACAGAGCAT GCCCATGTGA GAATGCTAAG TGTCCTTCAG 
ATGGTCTTCT CCAAACCATT GGAGAGACAA GAACTCCTGG CCGCTACTGA GCTGGCTGCC 
ATCTTCCCCA GCCTCGATGA GATCATTGAA ATGCACTATA ACTTCTACGA GAACCTGAAA 
AAGCTGCGGC TGGATGACAG CTTCATAGTT AAATCCATCA GCACCACAGT GCTCAACAGA 
TTCGGTGGTA CAGAGGGTGA GTGGTTTCAG AAGCTGACAG CCCGGTTCTG CAGTCACCAG 
TCATGGGCCC TGGATCAGAT TAAGGGCAGA CAAAGGAAAG ACCCACGCTT CAATTCCTTT 
ATACAGGAGG CAGAGAGTAA GCCCCAGTGC CGCAGGCTGC AGCTCAAGGA CATTATTCCT 
ATAGAGATGC AGAGACTGAC CAAGTATCCA TTGCTGCTGG AGAATATTGC AAAGAACACA 
GAGAACCCAA CAGAGAAGGA GAGCATCCAG CAGAGTGCAG AGTGCTGCAG AAAAATCCTC 
AACCATGTCA ATGAGGAGGT CAAAGTAATG GAGAACCTGT TGACTGTGAA GGACTACCAG 
CGTAGATTGG ACACATCAGG TCTTAAACCC AGTAACGAAC TTTATACAGA ATACAAGAAC 
ATTGACTTGA CTCAGAAGAA GATGCTTTAT GAAGGCCCAC TGACCTGGAA AGTCACCAAA 
GAGAAGGCTA TTGAGGTGCA GTGTGTGTTG CTTGGTGACC TGCTAGTGCT CCTACAGAGG 
CAAGATGACA AGATGGTTCT CAAATGCCAG AGCAAAAGTA CCATCGCTGT GCAGGAGGGC 
AAGCAGATGC TGAGCCCCAT TATTAAGCTG GACTCAGTTT TCCTCCGTGA GGTGGCCACA 
GATCGAAAGG CTTTCTACGT GATATTTACC TGGGACAGTG GTGCTCAAAT CTATGAACTC 
GTGGCTCAGT CTGTTGGAGA AATGAAAACT TGGACTGAGG TGATAAAGTT AGCAGTAGAT 
GATCTGAAAA AGAGTGGTGC ACCTATGAGG CTGCCACTGC CTCCAGGAGG TGGAGGAGCT 
CCCTTCAGTC CCTCTACGCT AAATGCCCCT GTGAGCCTGA CTGAGAATGG AAGTTTGAAA 
AGCGGCGCTG ATCGAGACAA GTACGGTTTG AAGTCTACGG ATCCCCGGCA CAGGCTAATC 
GATTTCCTGT CAGATAAAGG CTTTGACTTG ATAGGCCACT CCAACAGCAT TCAGGAGAAG 
GTGGCCAGTC GTGCTTTGGA TGACGTCATG TCACTGAAAA GACTGTTAAT AGGCAGCATC 
AGTCTATCAG ACGACTCAAA ACCTGATGAA GAAAATGAAG AGGAACAATC AGAGAGGCCC 
GATCAAGAAA GGGATTGCTG TCAGTCAACA GAAGAAAATC CGACCAGAGA CGGAGGGGCT 
GAAGTGGTAA ACGTCAACAC ATGTAAAAAA GAAGGTGAAG AGATGAAAGG AGATGAAGAA 
AGGAGCATCA GTGCTCCCCT GGTTTTATCC CAGGAGAGGC TGGAGGAAGT GTACAGGAGG 
CTGCAGAGCC TGGAGGAACA GCTAGAGAGA CTACAGAATG TAGAAGAGGA GCACCACAGG 
CTGCAGGAGG CTCTTTCTGA GTTCTCACTG GAACGGGGTA ATTTCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_023263655.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023119423.1 Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OAm103085
Clone ID Related Accession (Same CDS sequence)	XM_023263655.1
Accession Version	XM_023263655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	rho guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376149.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATCA TCGGGGCCGA GGATGAAGAT TTTGAGAATG ATCTGAATGA TGTGATGGAT 
GACCAGTCCC CGCACTTCAA CAGCATAGAG GTGTTGAAGG AGCGGCCGAC CCACCTGCTG 
GTCTTCATGC AACATGTCAT TCTGCAGTTT GACCCTGCTC CCTTGCTGTG TTACCTCCAT 
GCTGACCTCT TCAGGAGCCT CAGTGCCAAA GAGACCAAGA AACAGTTTGT GGAGTTCTAC 
AATACCTTTT TGGACAAGGG TGCAATTCTC AGAGTGACAA TACCACCTTC AATATCATAT 
GAATTGGACC GGACCCGTCC AGATCTGTTC TCAGAAGAGA CCCAAAGGCG TTTTGCTCAG 
GAGGTTCAGA ACCTGCAGAC TGTTGAAGTG GCAAAACAGT TGGAGGATTT CAGGCAGAAG 
AGAAGGATGG GTATGACGCC CAATGAAGCT GAACTAATTG ATGTGGAGAG TCACTATCCC 
ACCGACCGCG TCCCAATGGA AATGAAGGAA AAGTCTGTGG CTGAGAACCT GCTGGAGAAG 
ATGTCTGAGA CACAACCTAC GATTGTCTCG GACGATGAAA AATGCCAATC CATCTTTACT 
GCAGTGGCCT TCTACATGAA GCACCTTGGA GTTAAAACCA GGGCAGTTGA CAGTAAGAAG 
TCTAGAGGAG GCTTCTTCAG GAGTAAACTG GTAAAGAATA AGAAGGATGA ATCTACAAAG 
CCCAAGCCCA GAGGGGTGTT TCCTGGCATC CCAAGCTGGA TTGGAGGCAA CACTGAGGTC 
AAACCTGAAA CGGAGGCTAA AGCGGAAAAG GAGAAAGTGG CTCTGGATCG TAAAGGCCCT 
GCTCCTGGCA GAGGCTCCTT GACCGACCCT GCAGTCCCTG CCCTCCCTAG CAGAAAGGCT 
GGTTCCAGTG GAAGCCCTGC CTCACTGCCT GGACTAGAGA TTAGTGATGG CTCGGGCAAC 
AACATCAGCA CTACCAATAG CCCAGAGTCT TTACACACTG ATGGTCTTTC AAGCAATCGT 
TTAGAGCCCC CAGTACTGTC AGACGGGGGT GATGTTTCCC CCGTCGGGAT TTCAGGTGGG 
CTAATCATTG GGGAGCCCAG CTCACCTTGT GACACACCCA CAGACGAGAC TGTGGAGAAA 
GACAGACGGA AGACAAGTAG GAAAGTGGGG CGCAGTGAGA GTGCACGTGT GGACCGGCAC 
TCGTATCGGC GCCGTGGCTC TTCTCGGGCT AAACAGTCTC GCTCCCGTAG TGATGTGGAC 
CTTCAGCCAC CTTCTACCAC GACAACACCA ACTGCCCTGC TCACCCCCCA GCAACTCCAT 
CCTGTTGATG GAACAGTTTT AGTTCCTGAA GGACCTGGCC ACTCCCCCAC CTGTCCCTCA 
CCACAGCTGG AGGAGATGGA GCCACGTCTC TTGGAGTTTG AGCAGGACCC ACCCAACTGG 
AGAGAGCTGG CCTCTCCTGA AGCCTTGTCC AGCCTCAGCA AAAAGGAGAC TAAAAGGCAG 
GAAGTTATTA ATGAGCTGAT TGCTACAGAG CATGCCCATG TGAGAATGCT AAGTGTCCTT 
CAGATGGTCT TCTCCAAACC ATTGGAGAGA CAAGAACTCC TGGCCGCTAC TGAGCTGGCT 
GCCATCTTCC CCAGCCTCGA TGAGATCATT GAAATGCACT ATAACTTCTA CGAGAACCTG 
AAAAAGCTGC GGCTGGATGA CAGCTTCATA GTTAAATCCA TCAGCACCAC AGTGCTCAAC 
AGATTCGGTG GTACAGAGGG TGAGTGGTTT CAGAAGCTGA CAGCCCGGTT CTGCAGTCAC 
CAGTCATGGG CCCTGGATCA GATTAAGGGC AGACAAAGGA AAGACCCACG CTTCAATTCC 
TTTATACAGG AGGCAGAGAG TAAGCCCCAG TGCCGCAGGC TGCAGCTCAA GGACATTATT 
CCTATAGAGA TGCAGAGACT GACCAAGTAT CCATTGCTGC TGGAGAATAT TGCAAAGAAC 
ACAGAGAACC CAACAGAGAA GGAGAGCATC CAGCAGAGTG CAGAGTGCTG CAGAAAAATC 
CTCAACCATG TCAATGAGGA GGTCAAAGTA ATGGAGAACC TGTTGACTGT GAAGGACTAC 
CAGCGTAGAT TGGACACATC AGGTCTTAAA CCCAGTAACG AACTTTATAC AGAATACAAG 
AACATTGACT TGACTCAGAA GAAGATGCTT TATGAAGGCC CACTGACCTG GAAAGTCACC 
AAAGAGAAGG CTATTGAGGT GCAGTGTGTG TTGCTTGGTG ACCTGCTAGT GCTCCTACAG 
AGGCAAGATG ACAAGATGGT TCTCAAATGC CAGAGCAAAA GTACCATCGC TGTGCAGGAG 
GGCAAGCAGA TGCTGAGCCC CATTATTAAG CTGGACTCAG TTTTCCTCCG TGAGGTGGCC 
ACAGATCGAA AGGCTTTCTA CGTGATATTT ACCTGGGACA GTGGTGCTCA AATCTATGAA 
CTCGTGGCTC AGTCTGTTGG AGAAATGAAA ACTTGGACTG AGGTGATAAA GTTAGCAGTA 
GATGATCTGA AAAAGAGTGG TGCACCTATG AGGCTGCCAC TGCCTCCAGG AGGTGGAGGA 
GCTCCCTTCA GTCCCTCTAC GCTAAATGCC CCTGTGAGCC TGACTGAGAA TGGAAGTTTG 
AAAAGCGGCG CTGATCGAGA CAAGTACGGT TTGAAGTCTA CGGATCCCCG GCACAGGCTA 
ATCGATTTCC TGTCAGATAA AGGCTTTGAC TTGATAGGCC ACTCCAACAG CATTCAGGAG 
AAGGTGGCCA GTCGTGCTTT GGATGACGTC ATGTCACTGA AAAGACTGTT AATAGGCAGC 
ATCAGTCTAT CAGACGACTC AAAACCTGAT GAAGAAAATG AAGAGGAACA ATCAGAGAGG 
CCCGATCAAG AAAGGGATTG CTGTCAGTCA ACAGAAAATC CGACCAGAGA CGGAGGGGCT 
GAAGTGGTAA ACGTCAACAC ATGTAAAAAA GAAGGTGAAG AGATGAAAGG AGATGAAGAA 
AGGAGCATCA GTGCTCCCCT GGTTTTATCC CAGGAGAGGC TGGAGGAAGT GTACAGGAGG 
CTGCAGAGCC TGGAGGAACA GCTAGAGAGA CTACAGAATG TAGAAGAGGA GCACCACAGG 
CTGCAGGAGG CTCTTTCTGA GTTCTCACTG GAACGGGGTA ATTTCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023119423.1
CDS	107..3277
Translation	MSIIGAEDEDFENDLNDVMDDQSPHFNSIEVLKERPTHLLVFMQHVILQFDPAPLLCYLHADLFRSLSAKETKKQFVEFYNTFLDKGAILRVTIPPSISYELDRTRPDLFSEETQRRFAQEVQNLQTVEVAKQLEDFRQKRRMGMTPNEAELIDVESHYPTDRVPMEMKEKSVAENLLEKMSETQPTIVSDDEKCQSIFTAVAFYMKHLGVKTRAVDSKKSRGGFFRSKLVKNKKDESTKPKPRGVFPGIPSWIGGNTEVKPETEAKAEKEKVALDRKGPAPGRGSLTDPAVPALPSRKAGSSGSPASLPGLEISDGSGNNISTTNSPESLHTDGLSSNRLEPPVLSDGGDVSPVGISGGLIIGEPSSPCDTPTDETVEKDRRKTSRKVGRSESARVDRHSYRRRGSSRAKQSRSRSDVDLQPPSTTTTPTALLTPQQLHPVDGTVLVPEGPGHSPTCPSPQLEEMEPRLLEFEQDPPNWRELASPEALSSLSKKETKRQEVINELIATEHAHVRMLSVLQMVFSKPLERQELLAATELAAIFPSLDEIIEMHYNFYENLKKLRLDDSFIVKSISTTVLNRFGGTEGEWFQKLTARFCSHQSWALDQIKGRQRKDPRFNSFIQEAESKPQCRRLQLKDIIPIEMQRLTKYPLLLENIAKNTENPTEKESIQQSAECCRKILNHVNEEVKVMENLLTVKDYQRRLDTSGLKPSNELYTEYKNIDLTQKKMLYEGPLTWKVTKEKAIEVQCVLLGDLLVLLQRQDDKMVLKCQSKSTIAVQEGKQMLSPIIKLDSVFLREVATDRKAFYVIFTWDSGAQIYELVAQSVGEMKTWTEVIKLAVDDLKKSGAPMRLPLPPGGGGAPFSPSTLNAPVSLTENGSLKSGADRDKYGLKSTDPRHRLIDFLSDKGFDLIGHSNSIQEKVASRALDDVMSLKRLLIGSISLSDDSKPDEENEEEQSERPDQERDCCQSTENPTRDGGAEVVNVNTCKKEGEEMKGDEERSISAPLVLSQERLEEVYRRLQSLEEQLERLQNVEEEHHRLQEALSEFSLERGNFQ

Target ORF information:

RefSeq Version	XM_023263655.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023263655.1

ORF Insert Sequence:

ATGAGCATCA TCGGGGCCGA GGATGAAGAT TTTGAGAATG ATCTGAATGA TGTGATGGAT 
GACCAGTCCC CGCACTTCAA CAGCATAGAG GTGTTGAAGG AGCGGCCGAC CCACCTGCTG 
GTCTTCATGC AACATGTCAT TCTGCAGTTT GACCCTGCTC CCTTGCTGTG TTACCTCCAT 
GCTGACCTCT TCAGGAGCCT CAGTGCCAAA GAGACCAAGA AACAGTTTGT GGAGTTCTAC 
AATACCTTTT TGGACAAGGG TGCAATTCTC AGAGTGACAA TACCACCTTC AATATCATAT 
GAATTGGACC GGACCCGTCC AGATCTGTTC TCAGAAGAGA CCCAAAGGCG TTTTGCTCAG 
GAGGTTCAGA ACCTGCAGAC TGTTGAAGTG GCAAAACAGT TGGAGGATTT CAGGCAGAAG 
AGAAGGATGG GTATGACGCC CAATGAAGCT GAACTAATTG ATGTGGAGAG TCACTATCCC 
ACCGACCGCG TCCCAATGGA AATGAAGGAA AAGTCTGTGG CTGAGAACCT GCTGGAGAAG 
ATGTCTGAGA CACAACCTAC GATTGTCTCG GACGATGAAA AATGCCAATC CATCTTTACT 
GCAGTGGCCT TCTACATGAA GCACCTTGGA GTTAAAACCA GGGCAGTTGA CAGTAAGAAG 
TCTAGAGGAG GCTTCTTCAG GAGTAAACTG GTAAAGAATA AGAAGGATGA ATCTACAAAG 
CCCAAGCCCA GAGGGGTGTT TCCTGGCATC CCAAGCTGGA TTGGAGGCAA CACTGAGGTC 
AAACCTGAAA CGGAGGCTAA AGCGGAAAAG GAGAAAGTGG CTCTGGATCG TAAAGGCCCT 
GCTCCTGGCA GAGGCTCCTT GACCGACCCT GCAGTCCCTG CCCTCCCTAG CAGAAAGGCT 
GGTTCCAGTG GAAGCCCTGC CTCACTGCCT GGACTAGAGA TTAGTGATGG CTCGGGCAAC 
AACATCAGCA CTACCAATAG CCCAGAGTCT TTACACACTG ATGGTCTTTC AAGCAATCGT 
TTAGAGCCCC CAGTACTGTC AGACGGGGGT GATGTTTCCC CCGTCGGGAT TTCAGGTGGG 
CTAATCATTG GGGAGCCCAG CTCACCTTGT GACACACCCA CAGACGAGAC TGTGGAGAAA 
GACAGACGGA AGACAAGTAG GAAAGTGGGG CGCAGTGAGA GTGCACGTGT GGACCGGCAC 
TCGTATCGGC GCCGTGGCTC TTCTCGGGCT AAACAGTCTC GCTCCCGTAG TGATGTGGAC 
CTTCAGCCAC CTTCTACCAC GACAACACCA ACTGCCCTGC TCACCCCCCA GCAACTCCAT 
CCTGTTGATG GAACAGTTTT AGTTCCTGAA GGACCTGGCC ACTCCCCCAC CTGTCCCTCA 
CCACAGCTGG AGGAGATGGA GCCACGTCTC TTGGAGTTTG AGCAGGACCC ACCCAACTGG 
AGAGAGCTGG CCTCTCCTGA AGCCTTGTCC AGCCTCAGCA AAAAGGAGAC TAAAAGGCAG 
GAAGTTATTA ATGAGCTGAT TGCTACAGAG CATGCCCATG TGAGAATGCT AAGTGTCCTT 
CAGATGGTCT TCTCCAAACC ATTGGAGAGA CAAGAACTCC TGGCCGCTAC TGAGCTGGCT 
GCCATCTTCC CCAGCCTCGA TGAGATCATT GAAATGCACT ATAACTTCTA CGAGAACCTG 
AAAAAGCTGC GGCTGGATGA CAGCTTCATA GTTAAATCCA TCAGCACCAC AGTGCTCAAC 
AGATTCGGTG GTACAGAGGG TGAGTGGTTT CAGAAGCTGA CAGCCCGGTT CTGCAGTCAC 
CAGTCATGGG CCCTGGATCA GATTAAGGGC AGACAAAGGA AAGACCCACG CTTCAATTCC 
TTTATACAGG AGGCAGAGAG TAAGCCCCAG TGCCGCAGGC TGCAGCTCAA GGACATTATT 
CCTATAGAGA TGCAGAGACT GACCAAGTAT CCATTGCTGC TGGAGAATAT TGCAAAGAAC 
ACAGAGAACC CAACAGAGAA GGAGAGCATC CAGCAGAGTG CAGAGTGCTG CAGAAAAATC 
CTCAACCATG TCAATGAGGA GGTCAAAGTA ATGGAGAACC TGTTGACTGT GAAGGACTAC 
CAGCGTAGAT TGGACACATC AGGTCTTAAA CCCAGTAACG AACTTTATAC AGAATACAAG 
AACATTGACT TGACTCAGAA GAAGATGCTT TATGAAGGCC CACTGACCTG GAAAGTCACC 
AAAGAGAAGG CTATTGAGGT GCAGTGTGTG TTGCTTGGTG ACCTGCTAGT GCTCCTACAG 
AGGCAAGATG ACAAGATGGT TCTCAAATGC CAGAGCAAAA GTACCATCGC TGTGCAGGAG 
GGCAAGCAGA TGCTGAGCCC CATTATTAAG CTGGACTCAG TTTTCCTCCG TGAGGTGGCC 
ACAGATCGAA AGGCTTTCTA CGTGATATTT ACCTGGGACA GTGGTGCTCA AATCTATGAA 
CTCGTGGCTC AGTCTGTTGG AGAAATGAAA ACTTGGACTG AGGTGATAAA GTTAGCAGTA 
GATGATCTGA AAAAGAGTGG TGCACCTATG AGGCTGCCAC TGCCTCCAGG AGGTGGAGGA 
GCTCCCTTCA GTCCCTCTAC GCTAAATGCC CCTGTGAGCC TGACTGAGAA TGGAAGTTTG 
AAAAGCGGCG CTGATCGAGA CAAGTACGGT TTGAAGTCTA CGGATCCCCG GCACAGGCTA 
ATCGATTTCC TGTCAGATAA AGGCTTTGAC TTGATAGGCC ACTCCAACAG CATTCAGGAG 
AAGGTGGCCA GTCGTGCTTT GGATGACGTC ATGTCACTGA AAAGACTGTT AATAGGCAGC 
ATCAGTCTAT CAGACGACTC AAAACCTGAT GAAGAAAATG AAGAGGAACA ATCAGAGAGG 
CCCGATCAAG AAAGGGATTG CTGTCAGTCA ACAGAAAATC CGACCAGAGA CGGAGGGGCT 
GAAGTGGTAA ACGTCAACAC ATGTAAAAAA GAAGGTGAAG AGATGAAAGG AGATGAAGAA 
AGGAGCATCA GTGCTCCCCT GGTTTTATCC CAGGAGAGGC TGGAGGAAGT GTACAGGAGG 
CTGCAGAGCC TGGAGGAACA GCTAGAGAGA CTACAGAATG TAGAAGAGGA GCACCACAGG 
CTGCAGGAGG CTCTTTCTGA GTTCTCACTG GAACGGGGTA ATTTCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_023263656.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023119424.1 Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OAm103086
Clone ID Related Accession (Same CDS sequence)	XM_023263656.1
Accession Version	XM_023263656.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	rho guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376149.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATCA TCGGGGCCGA GGATGAAGAT TTTGAGAATG ATCTGAATGA TGTGATGGAT 
GACCAGTCCC CGCACTTCAA CAGCATAGAG GTGTTGAAGG AGCGGCCGAC CCACCTGCTG 
GTCTTCATGC AACATGTCAT TCTGCAGTTT GACCCTGCTC CCTTGCTGTG TTACCTCCAT 
GCTGACCTCT TCAGGAGCCT CAGTGCCAAA GAGACCAAGA AACAGTTTGT GGAGTTCTAC 
AATACCTTTT TGGACAAGGG TGCAATTCTC AGAGTGACAA TACCACCTTC AATATCATAT 
GAATTGGACC GGACCCGTCC AGATCTGTTC TCAGAAGAGA CCCAAAGGCG TTTTGCTCAG 
GAGGTTCAGA ACCTGCAGAC TGTTGAAGTG GCAAAACAGT TGGAGGATTT CAGGCAGAAG 
AGAAGGATGG GTATGACGCC CAATGAAGCT GAACTAATTG ATGTGGAGAG TCACTATCCC 
ACCGACCGCG TCCCAATGGA AATGAAGGAA AAGTCTGTGG CTGAGAACCT GCTGGAGAAG 
ATGTCTGAGA CACAACCTAC GATTGTCTCG GACGATGAAA AATGCCAATC CATCTTTACT 
GCAGTGGCCT TCTACATGAA GCACCTTGGA GTTAAAACCA GGGCAGTTGA CAGTAAGAAG 
TCTAGAGGAG GCTTCTTCAG GAGTAAACTG GTAAAGAATA AGAAGGATGA ATCTACAAAG 
CCCAAGCCCA GAGGGGTGTT TCCTGGCATC CCAAGCTGGA TTGGAGGCAA CACTGAGGTC 
AAACCTGAAA CGGAGGCTAA AGCGGAAAAG GAGAAAGTGG CTCTGGATCG TAAAGGCCCT 
GCTCCTGGCA GAGGCTCCTT GACCGACCCT GCAGTCCCTG CCCTCCCTAG CAGAAAGGCT 
GGTTCCAGTG GAAGCCCTGC CTCACTGCCT GGACTAGAGA TTAGTGATGG CTCGGGCAAC 
AACATCAGCA CTACCAATAG CCCAGAGTCT TTACACACTG ATGGTCTTTC AAGCAATCGT 
TTAGAGCCCC CAGTACTGTC AGACGGGGGT GATGTTTCCC CCGTCGGGAT TTCAGGTGGG 
CTAATCATTG GGGAGCCCAG CTCACCTTGT GACACACCCA CAGACGAGAC TGTGGAGAAA 
GACAGTAGGA AAGTGGGGCG CAGTGAGAGT GCACGTGTGG ACCGGCACTC GTATCGGCGC 
CGTGGCTCTT CTCGGGCTAA ACAGTCTCGC TCCCGTAGTG ATGTGGACCT TCAGCCACCT 
TCTACCACGA CAACACCAAC TGCCCTGCTC ACCCCCCAGC AACTCCATCC TGTTGATGGA 
ACAGTTTTAG TTCCTGAAGG ACCTGGCCAC TCCCCCACCT GTCCCTCACC ACAGCTGGAG 
GAGATGGAGC CACGTCTCTT GGAGTTTGAG CAGGACCCAC CCAACTGGAG AGAGCTGGCC 
TCTCCTGAAG CCTTGTCCAG CCTCAGCAAA AAGGAGACTA AAAGGCAGGA AGTTATTAAT 
GAGCTGATTG CTACAGAGCA TGCCCATGTG AGAATGCTAA GTGTCCTTCA GATGGTCTTC 
TCCAAACCAT TGGAGAGACA AGAACTCCTG GCCGCTACTG AGCTGGCTGC CATCTTCCCC 
AGCCTCGATG AGATCATTGA AATGCACTAT AACTTCTACG AGAACCTGAA AAAGCTGCGG 
CTGGATGACA GCTTCATAGT TAAATCCATC AGCACCACAG TGCTCAACAG ATTCGGTGGT 
ACAGAGGGTG AGTGGTTTCA GAAGCTGACA GCCCGGTTCT GCAGTCACCA GTCATGGGCC 
CTGGATCAGA TTAAGGGCAG ACAAAGGAAA GACCCACGCT TCAATTCCTT TATACAGGAG 
GCAGAGAGTA AGCCCCAGTG CCGCAGGCTG CAGCTCAAGG ACATTATTCC TATAGAGATG 
CAGAGACTGA CCAAGTATCC ATTGCTGCTG GAGAATATTG CAAAGAACAC AGAGAACCCA 
ACAGAGAAGG AGAGCATCCA GCAGAGTGCA GAGTGCTGCA GAAAAATCCT CAACCATGTC 
AATGAGGAGG TCAAAGTAAT GGAGAACCTG TTGACTGTGA AGGACTACCA GCGTAGATTG 
GACACATCAG GTCTTAAACC CAGTAACGAA CTTTATACAG AATACAAGAA CATTGACTTG 
ACTCAGAAGA AGATGCTTTA TGAAGGCCCA CTGACCTGGA AAGTCACCAA AGAGAAGGCT 
ATTGAGGTGC AGTGTGTGTT GCTTGGTGAC CTGCTAGTGC TCCTACAGAG GCAAGATGAC 
AAGATGGTTC TCAAATGCCA GAGCAAAAGT ACCATCGCTG TGCAGGAGGG CAAGCAGATG 
CTGAGCCCCA TTATTAAGCT GGACTCAGTT TTCCTCCGTG AGGTGGCCAC AGATCGAAAG 
GCTTTCTACG TGATATTTAC CTGGGACAGT GGTGCTCAAA TCTATGAACT CGTGGCTCAG 
TCTGTTGGAG AAATGAAAAC TTGGACTGAG GTGATAAAGT TAGCAGTAGA TGATCTGAAA 
AAGAGTGGTG CACCTATGAG GCTGCCACTG CCTCCAGGAG GTGGAGGAGC TCCCTTCAGT 
CCCTCTACGC TAAATGCCCC TGTGAGCCTG ACTGAGAATG GAAGTTTGAA AAGCGGCGCT 
GATCGAGACA AGTACGGTTT GAAGTCTACG GATCCCCGGC ACAGGCTAAT CGATTTCCTG 
TCAGATAAAG GCTTTGACTT GATAGGCCAC TCCAACAGCA TTCAGGAGAA GGTGGCCAGT 
CGTGCTTTGG ATGACGTCAT GTCACTGAAA AGACTGTTAA TAGGCAGCAT CAGTCTATCA 
GACGACTCAA AACCTGATGA AGAAAATGAA GAGGAACAAT CAGAGAGGCC CGATCAAGAA 
AGGGATTGCT GTCAGTCAAC AGAAGAAAAT CCGACCAGAG ACGGAGGGGC TGAAGTGGTA 
AACGTCAACA CATGTAAAAA AGAAGGTGAA GAGATGAAAG GAGATGAAGA AAGGAGCATC 
AGTGCTCCCC TGGTTTTATC CCAGGAGAGG CTGGAGGAAG TGTACAGGAG GCTGCAGAGC 
CTGGAGGAAC AGCTAGAGAG ACTACAGAAT GTAGAAGAGG AGCACCACAG GCTGCAGGAG 
GCTCTTTCTG AGTTCTCACT GGAACGGGGT AATTTCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023119424.1
CDS	107..3268
Translation	MSIIGAEDEDFENDLNDVMDDQSPHFNSIEVLKERPTHLLVFMQHVILQFDPAPLLCYLHADLFRSLSAKETKKQFVEFYNTFLDKGAILRVTIPPSISYELDRTRPDLFSEETQRRFAQEVQNLQTVEVAKQLEDFRQKRRMGMTPNEAELIDVESHYPTDRVPMEMKEKSVAENLLEKMSETQPTIVSDDEKCQSIFTAVAFYMKHLGVKTRAVDSKKSRGGFFRSKLVKNKKDESTKPKPRGVFPGIPSWIGGNTEVKPETEAKAEKEKVALDRKGPAPGRGSLTDPAVPALPSRKAGSSGSPASLPGLEISDGSGNNISTTNSPESLHTDGLSSNRLEPPVLSDGGDVSPVGISGGLIIGEPSSPCDTPTDETVEKDSRKVGRSESARVDRHSYRRRGSSRAKQSRSRSDVDLQPPSTTTTPTALLTPQQLHPVDGTVLVPEGPGHSPTCPSPQLEEMEPRLLEFEQDPPNWRELASPEALSSLSKKETKRQEVINELIATEHAHVRMLSVLQMVFSKPLERQELLAATELAAIFPSLDEIIEMHYNFYENLKKLRLDDSFIVKSISTTVLNRFGGTEGEWFQKLTARFCSHQSWALDQIKGRQRKDPRFNSFIQEAESKPQCRRLQLKDIIPIEMQRLTKYPLLLENIAKNTENPTEKESIQQSAECCRKILNHVNEEVKVMENLLTVKDYQRRLDTSGLKPSNELYTEYKNIDLTQKKMLYEGPLTWKVTKEKAIEVQCVLLGDLLVLLQRQDDKMVLKCQSKSTIAVQEGKQMLSPIIKLDSVFLREVATDRKAFYVIFTWDSGAQIYELVAQSVGEMKTWTEVIKLAVDDLKKSGAPMRLPLPPGGGGAPFSPSTLNAPVSLTENGSLKSGADRDKYGLKSTDPRHRLIDFLSDKGFDLIGHSNSIQEKVASRALDDVMSLKRLLIGSISLSDDSKPDEENEEEQSERPDQERDCCQSTEENPTRDGGAEVVNVNTCKKEGEEMKGDEERSISAPLVLSQERLEEVYRRLQSLEEQLERLQNVEEEHHRLQEALSEFSLERGNFQ

Target ORF information:

RefSeq Version	XM_023263656.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023263656.1

ORF Insert Sequence:

ATGAGCATCA TCGGGGCCGA GGATGAAGAT TTTGAGAATG ATCTGAATGA TGTGATGGAT 
GACCAGTCCC CGCACTTCAA CAGCATAGAG GTGTTGAAGG AGCGGCCGAC CCACCTGCTG 
GTCTTCATGC AACATGTCAT TCTGCAGTTT GACCCTGCTC CCTTGCTGTG TTACCTCCAT 
GCTGACCTCT TCAGGAGCCT CAGTGCCAAA GAGACCAAGA AACAGTTTGT GGAGTTCTAC 
AATACCTTTT TGGACAAGGG TGCAATTCTC AGAGTGACAA TACCACCTTC AATATCATAT 
GAATTGGACC GGACCCGTCC AGATCTGTTC TCAGAAGAGA CCCAAAGGCG TTTTGCTCAG 
GAGGTTCAGA ACCTGCAGAC TGTTGAAGTG GCAAAACAGT TGGAGGATTT CAGGCAGAAG 
AGAAGGATGG GTATGACGCC CAATGAAGCT GAACTAATTG ATGTGGAGAG TCACTATCCC 
ACCGACCGCG TCCCAATGGA AATGAAGGAA AAGTCTGTGG CTGAGAACCT GCTGGAGAAG 
ATGTCTGAGA CACAACCTAC GATTGTCTCG GACGATGAAA AATGCCAATC CATCTTTACT 
GCAGTGGCCT TCTACATGAA GCACCTTGGA GTTAAAACCA GGGCAGTTGA CAGTAAGAAG 
TCTAGAGGAG GCTTCTTCAG GAGTAAACTG GTAAAGAATA AGAAGGATGA ATCTACAAAG 
CCCAAGCCCA GAGGGGTGTT TCCTGGCATC CCAAGCTGGA TTGGAGGCAA CACTGAGGTC 
AAACCTGAAA CGGAGGCTAA AGCGGAAAAG GAGAAAGTGG CTCTGGATCG TAAAGGCCCT 
GCTCCTGGCA GAGGCTCCTT GACCGACCCT GCAGTCCCTG CCCTCCCTAG CAGAAAGGCT 
GGTTCCAGTG GAAGCCCTGC CTCACTGCCT GGACTAGAGA TTAGTGATGG CTCGGGCAAC 
AACATCAGCA CTACCAATAG CCCAGAGTCT TTACACACTG ATGGTCTTTC AAGCAATCGT 
TTAGAGCCCC CAGTACTGTC AGACGGGGGT GATGTTTCCC CCGTCGGGAT TTCAGGTGGG 
CTAATCATTG GGGAGCCCAG CTCACCTTGT GACACACCCA CAGACGAGAC TGTGGAGAAA 
GACAGTAGGA AAGTGGGGCG CAGTGAGAGT GCACGTGTGG ACCGGCACTC GTATCGGCGC 
CGTGGCTCTT CTCGGGCTAA ACAGTCTCGC TCCCGTAGTG ATGTGGACCT TCAGCCACCT 
TCTACCACGA CAACACCAAC TGCCCTGCTC ACCCCCCAGC AACTCCATCC TGTTGATGGA 
ACAGTTTTAG TTCCTGAAGG ACCTGGCCAC TCCCCCACCT GTCCCTCACC ACAGCTGGAG 
GAGATGGAGC CACGTCTCTT GGAGTTTGAG CAGGACCCAC CCAACTGGAG AGAGCTGGCC 
TCTCCTGAAG CCTTGTCCAG CCTCAGCAAA AAGGAGACTA AAAGGCAGGA AGTTATTAAT 
GAGCTGATTG CTACAGAGCA TGCCCATGTG AGAATGCTAA GTGTCCTTCA GATGGTCTTC 
TCCAAACCAT TGGAGAGACA AGAACTCCTG GCCGCTACTG AGCTGGCTGC CATCTTCCCC 
AGCCTCGATG AGATCATTGA AATGCACTAT AACTTCTACG AGAACCTGAA AAAGCTGCGG 
CTGGATGACA GCTTCATAGT TAAATCCATC AGCACCACAG TGCTCAACAG ATTCGGTGGT 
ACAGAGGGTG AGTGGTTTCA GAAGCTGACA GCCCGGTTCT GCAGTCACCA GTCATGGGCC 
CTGGATCAGA TTAAGGGCAG ACAAAGGAAA GACCCACGCT TCAATTCCTT TATACAGGAG 
GCAGAGAGTA AGCCCCAGTG CCGCAGGCTG CAGCTCAAGG ACATTATTCC TATAGAGATG 
CAGAGACTGA CCAAGTATCC ATTGCTGCTG GAGAATATTG CAAAGAACAC AGAGAACCCA 
ACAGAGAAGG AGAGCATCCA GCAGAGTGCA GAGTGCTGCA GAAAAATCCT CAACCATGTC 
AATGAGGAGG TCAAAGTAAT GGAGAACCTG TTGACTGTGA AGGACTACCA GCGTAGATTG 
GACACATCAG GTCTTAAACC CAGTAACGAA CTTTATACAG AATACAAGAA CATTGACTTG 
ACTCAGAAGA AGATGCTTTA TGAAGGCCCA CTGACCTGGA AAGTCACCAA AGAGAAGGCT 
ATTGAGGTGC AGTGTGTGTT GCTTGGTGAC CTGCTAGTGC TCCTACAGAG GCAAGATGAC 
AAGATGGTTC TCAAATGCCA GAGCAAAAGT ACCATCGCTG TGCAGGAGGG CAAGCAGATG 
CTGAGCCCCA TTATTAAGCT GGACTCAGTT TTCCTCCGTG AGGTGGCCAC AGATCGAAAG 
GCTTTCTACG TGATATTTAC CTGGGACAGT GGTGCTCAAA TCTATGAACT CGTGGCTCAG 
TCTGTTGGAG AAATGAAAAC TTGGACTGAG GTGATAAAGT TAGCAGTAGA TGATCTGAAA 
AAGAGTGGTG CACCTATGAG GCTGCCACTG CCTCCAGGAG GTGGAGGAGC TCCCTTCAGT 
CCCTCTACGC TAAATGCCCC TGTGAGCCTG ACTGAGAATG GAAGTTTGAA AAGCGGCGCT 
GATCGAGACA AGTACGGTTT GAAGTCTACG GATCCCCGGC ACAGGCTAAT CGATTTCCTG 
TCAGATAAAG GCTTTGACTT GATAGGCCAC TCCAACAGCA TTCAGGAGAA GGTGGCCAGT 
CGTGCTTTGG ATGACGTCAT GTCACTGAAA AGACTGTTAA TAGGCAGCAT CAGTCTATCA 
GACGACTCAA AACCTGATGA AGAAAATGAA GAGGAACAAT CAGAGAGGCC CGATCAAGAA 
AGGGATTGCT GTCAGTCAAC AGAAGAAAAT CCGACCAGAG ACGGAGGGGC TGAAGTGGTA 
AACGTCAACA CATGTAAAAA AGAAGGTGAA GAGATGAAAG GAGATGAAGA AAGGAGCATC 
AGTGCTCCCC TGGTTTTATC CCAGGAGAGG CTGGAGGAAG TGTACAGGAG GCTGCAGAGC 
CTGGAGGAAC AGCTAGAGAG ACTACAGAAT GTAGAAGAGG AGCACCACAG GCTGCAGGAG 
GCTCTTTCTG AGTTCTCACT GGAACGGGGT AATTTCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_023263657.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023119425.1 Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OAm103087
Clone ID Related Accession (Same CDS sequence)	XM_023263657.1
Accession Version	XM_023263657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	rho guanine nucleotide exchange factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376149.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATCA TCGGGGCCGA GGATGAAGAT TTTGAGAATG ATCTGAATGA TGTGATGGAT 
GACCAGTCCC CGCACTTCAA CAGCATAGAG GTGTTGAAGG AGCGGCCGAC CCACCTGCTG 
GTCTTCATGC AACATGTCAT TCTGCAGTTT GACCCTGCTC CCTTGCTGTG TTACCTCCAT 
GCTGACCTCT TCAGGAGCCT CAGTGCCAAA GAGACCAAGA AACAGTTTGT GGAGTTCTAC 
AATACCTTTT TGGACAAGGG TGCAATTCTC AGAGTGACAA TACCACCTTC AATATCATAT 
GAATTGGACC GGACCCGTCC AGATCTGTTC TCAGAAGAGA CCCAAAGGCG TTTTGCTCAG 
GAGGTTCAGA ACCTGCAGAC TGTTGAAGTG GCAAAACAGT TGGAGGATTT CAGGCAGAAG 
AGAAGGATGG GTATGACGCC CAATGAAGCT GAACTAATTG ATGTGGAGAG TCACTATCCC 
ACCGACCGCG TCCCAATGGA AATGAAGGAA AAGTCTGTGG CTGAGAACCT GCTGGAGAAG 
ATGTCTGAGA CACAACCTAC GATTGTCTCG GACGATGAAA AATGCCAATC CATCTTTACT 
GCAGTGGCCT TCTACATGAA GCACCTTGGA GTTAAAACCA GGGCAGTTGA CAGTAAGAAG 
TCTAGAGGAG GCTTCTTCAG GAGTAAACTG GTAAAGAATA AGAAGGATGA ATCTACAAAG 
CCCAAGCCCA GAGGGGTGTT TCCTGGCATC CCAAGCTGGA TTGGAGGCAA CACTGAGGTC 
AAACCTGAAA CGGAGGCTAA AGCGGAAAAG GAGAAAGTGG CTCTGGATCG TAAAGGCCCT 
GCTCCTGGCA GAGGCTCCTT GACCGACCCT GCAGTCCCTG CCCTCCCTAG CAGAAAGGCT 
GGTTCCAGTG GAAGCCCTGC CTCACTGCCT GGACTAGAGA TTAGTGATGG CTCGGGCAAC 
AACATCAGCA CTACCAATAG CCCAGAGTCT TTACACACTG ATGGTCTTTC AAGCAATCGT 
TTAGAGCCCC CAGTACTGTC AGACGGGGGT GATGTTTCCC CCGTCGGGAT TTCAGGTGGG 
CTAATCATTG GGGAGCCCAG CTCACCTTGT GACACACCCA CAGACGAGAC TGTGGAGAAA 
GACAGGAAAG TGGGGCGCAG TGAGAGTGCA CGTGTGGACC GGCACTCGTA TCGGCGCCGT 
GGCTCTTCTC GGGCTAAACA GTCTCGCTCC CGTAGTGATG TGGACCTTCA GCCACCTTCT 
ACCACGACAA CACCAACTGC CCTGCTCACC CCCCAGCAAC TCCATCCTGT TGATGGAACA 
GTTTTAGTTC CTGAAGGACC TGGCCACTCC CCCACCTGTC CCTCACCACA GCTGGAGGAG 
ATGGAGCCAC GTCTCTTGGA GTTTGAGCAG GACCCACCCA ACTGGAGAGA GCTGGCCTCT 
CCTGAAGCCT TGTCCAGCCT CAGCAAAAAG GAGACTAAAA GGCAGGAAGT TATTAATGAG 
CTGATTGCTA CAGAGCATGC CCATGTGAGA ATGCTAAGTG TCCTTCAGAT GGTCTTCTCC 
AAACCATTGG AGAGACAAGA ACTCCTGGCC GCTACTGAGC TGGCTGCCAT CTTCCCCAGC 
CTCGATGAGA TCATTGAAAT GCACTATAAC TTCTACGAGA ACCTGAAAAA GCTGCGGCTG 
GATGACAGCT TCATAGTTAA ATCCATCAGC ACCACAGTGC TCAACAGATT CGGTGGTACA 
GAGGGTGAGT GGTTTCAGAA GCTGACAGCC CGGTTCTGCA GTCACCAGTC ATGGGCCCTG 
GATCAGATTA AGGGCAGACA AAGGAAAGAC CCACGCTTCA ATTCCTTTAT ACAGGAGGCA 
GAGAGTAAGC CCCAGTGCCG CAGGCTGCAG CTCAAGGACA TTATTCCTAT AGAGATGCAG 
AGACTGACCA AGTATCCATT GCTGCTGGAG AATATTGCAA AGAACACAGA GAACCCAACA 
GAGAAGGAGA GCATCCAGCA GAGTGCAGAG TGCTGCAGAA AAATCCTCAA CCATGTCAAT 
GAGGAGGTCA AAGTAATGGA GAACCTGTTG ACTGTGAAGG ACTACCAGCG TAGATTGGAC 
ACATCAGGTC TTAAACCCAG TAACGAACTT TATACAGAAT ACAAGAACAT TGACTTGACT 
CAGAAGAAGA TGCTTTATGA AGGCCCACTG ACCTGGAAAG TCACCAAAGA GAAGGCTATT 
GAGGTGCAGT GTGTGTTGCT TGGTGACCTG CTAGTGCTCC TACAGAGGCA AGATGACAAG 
ATGGTTCTCA AATGCCAGAG CAAAAGTACC ATCGCTGTGC AGGAGGGCAA GCAGATGCTG 
AGCCCCATTA TTAAGCTGGA CTCAGTTTTC CTCCGTGAGG TGGCCACAGA TCGAAAGGCT 
TTCTACGTGA TATTTACCTG GGACAGTGGT GCTCAAATCT ATGAACTCGT GGCTCAGTCT 
GTTGGAGAAA TGAAAACTTG GACTGAGGTG ATAAAGTTAG CAGTAGATGA TCTGAAAAAG 
AGTGGTGCAC CTATGAGGCT GCCACTGCCT CCAGGAGGTG GAGGAGCTCC CTTCAGTCCC 
TCTACGCTAA ATGCCCCTGT GAGCCTGACT GAGAATGGAA GTTTGAAAAG CGGCGCTGAT 
CGAGACAAGT ACGGTTTGAA GTCTACGGAT CCCCGGCACA GGCTAATCGA TTTCCTGTCA 
GATAAAGGCT TTGACTTGAT AGGCCACTCC AACAGCATTC AGGAGAAGGT GGCCAGTCGT 
GCTTTGGATG ACGTCATGTC ACTGAAAAGA CTGTTAATAG GCAGCATCAG TCTATCAGAC 
GACTCAAAAC CTGATGAAGA AAATGAAGAG GAACAATCAG AGAGGCCCGA TCAAGAAAGG 
GATTGCTGTC AGTCAACAGA AGAAAATCCG ACCAGAGACG GAGGGGCTGA AGTGGTAAAC 
GTCAACACAT GTAAAAAAGA AGGTGAAGAG ATGAAAGGAG ATGAAGAAAG GAGCATCAGT 
GCTCCCCTGG TTTTATCCCA GGAGAGGCTG GAGGAAGTGT ACAGGAGGCT GCAGAGCCTG 
GAGGAACAGC TAGAGAGACT ACAGAATGTA GAAGAGGAGC ACCACAGGCT GCAGGAGGCT 
CTTTCTGAGT TCTCACTGGA ACGGGGTAAT TTCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023119425.1
CDS	107..3265
Translation	MSIIGAEDEDFENDLNDVMDDQSPHFNSIEVLKERPTHLLVFMQHVILQFDPAPLLCYLHADLFRSLSAKETKKQFVEFYNTFLDKGAILRVTIPPSISYELDRTRPDLFSEETQRRFAQEVQNLQTVEVAKQLEDFRQKRRMGMTPNEAELIDVESHYPTDRVPMEMKEKSVAENLLEKMSETQPTIVSDDEKCQSIFTAVAFYMKHLGVKTRAVDSKKSRGGFFRSKLVKNKKDESTKPKPRGVFPGIPSWIGGNTEVKPETEAKAEKEKVALDRKGPAPGRGSLTDPAVPALPSRKAGSSGSPASLPGLEISDGSGNNISTTNSPESLHTDGLSSNRLEPPVLSDGGDVSPVGISGGLIIGEPSSPCDTPTDETVEKDRKVGRSESARVDRHSYRRRGSSRAKQSRSRSDVDLQPPSTTTTPTALLTPQQLHPVDGTVLVPEGPGHSPTCPSPQLEEMEPRLLEFEQDPPNWRELASPEALSSLSKKETKRQEVINELIATEHAHVRMLSVLQMVFSKPLERQELLAATELAAIFPSLDEIIEMHYNFYENLKKLRLDDSFIVKSISTTVLNRFGGTEGEWFQKLTARFCSHQSWALDQIKGRQRKDPRFNSFIQEAESKPQCRRLQLKDIIPIEMQRLTKYPLLLENIAKNTENPTEKESIQQSAECCRKILNHVNEEVKVMENLLTVKDYQRRLDTSGLKPSNELYTEYKNIDLTQKKMLYEGPLTWKVTKEKAIEVQCVLLGDLLVLLQRQDDKMVLKCQSKSTIAVQEGKQMLSPIIKLDSVFLREVATDRKAFYVIFTWDSGAQIYELVAQSVGEMKTWTEVIKLAVDDLKKSGAPMRLPLPPGGGGAPFSPSTLNAPVSLTENGSLKSGADRDKYGLKSTDPRHRLIDFLSDKGFDLIGHSNSIQEKVASRALDDVMSLKRLLIGSISLSDDSKPDEENEEEQSERPDQERDCCQSTEENPTRDGGAEVVNVNTCKKEGEEMKGDEERSISAPLVLSQERLEEVYRRLQSLEEQLERLQNVEEEHHRLQEALSEFSLERGNFQ

Target ORF information:

RefSeq Version	XM_023263657.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023263657.1

ORF Insert Sequence:

ATGAGCATCA TCGGGGCCGA GGATGAAGAT TTTGAGAATG ATCTGAATGA TGTGATGGAT 
GACCAGTCCC CGCACTTCAA CAGCATAGAG GTGTTGAAGG AGCGGCCGAC CCACCTGCTG 
GTCTTCATGC AACATGTCAT TCTGCAGTTT GACCCTGCTC CCTTGCTGTG TTACCTCCAT 
GCTGACCTCT TCAGGAGCCT CAGTGCCAAA GAGACCAAGA AACAGTTTGT GGAGTTCTAC 
AATACCTTTT TGGACAAGGG TGCAATTCTC AGAGTGACAA TACCACCTTC AATATCATAT 
GAATTGGACC GGACCCGTCC AGATCTGTTC TCAGAAGAGA CCCAAAGGCG TTTTGCTCAG 
GAGGTTCAGA ACCTGCAGAC TGTTGAAGTG GCAAAACAGT TGGAGGATTT CAGGCAGAAG 
AGAAGGATGG GTATGACGCC CAATGAAGCT GAACTAATTG ATGTGGAGAG TCACTATCCC 
ACCGACCGCG TCCCAATGGA AATGAAGGAA AAGTCTGTGG CTGAGAACCT GCTGGAGAAG 
ATGTCTGAGA CACAACCTAC GATTGTCTCG GACGATGAAA AATGCCAATC CATCTTTACT 
GCAGTGGCCT TCTACATGAA GCACCTTGGA GTTAAAACCA GGGCAGTTGA CAGTAAGAAG 
TCTAGAGGAG GCTTCTTCAG GAGTAAACTG GTAAAGAATA AGAAGGATGA ATCTACAAAG 
CCCAAGCCCA GAGGGGTGTT TCCTGGCATC CCAAGCTGGA TTGGAGGCAA CACTGAGGTC 
AAACCTGAAA CGGAGGCTAA AGCGGAAAAG GAGAAAGTGG CTCTGGATCG TAAAGGCCCT 
GCTCCTGGCA GAGGCTCCTT GACCGACCCT GCAGTCCCTG CCCTCCCTAG CAGAAAGGCT 
GGTTCCAGTG GAAGCCCTGC CTCACTGCCT GGACTAGAGA TTAGTGATGG CTCGGGCAAC 
AACATCAGCA CTACCAATAG CCCAGAGTCT TTACACACTG ATGGTCTTTC AAGCAATCGT 
TTAGAGCCCC CAGTACTGTC AGACGGGGGT GATGTTTCCC CCGTCGGGAT TTCAGGTGGG 
CTAATCATTG GGGAGCCCAG CTCACCTTGT GACACACCCA CAGACGAGAC TGTGGAGAAA 
GACAGGAAAG TGGGGCGCAG TGAGAGTGCA CGTGTGGACC GGCACTCGTA TCGGCGCCGT 
GGCTCTTCTC GGGCTAAACA GTCTCGCTCC CGTAGTGATG TGGACCTTCA GCCACCTTCT 
ACCACGACAA CACCAACTGC CCTGCTCACC CCCCAGCAAC TCCATCCTGT TGATGGAACA 
GTTTTAGTTC CTGAAGGACC TGGCCACTCC CCCACCTGTC CCTCACCACA GCTGGAGGAG 
ATGGAGCCAC GTCTCTTGGA GTTTGAGCAG GACCCACCCA ACTGGAGAGA GCTGGCCTCT 
CCTGAAGCCT TGTCCAGCCT CAGCAAAAAG GAGACTAAAA GGCAGGAAGT TATTAATGAG 
CTGATTGCTA CAGAGCATGC CCATGTGAGA ATGCTAAGTG TCCTTCAGAT GGTCTTCTCC 
AAACCATTGG AGAGACAAGA ACTCCTGGCC GCTACTGAGC TGGCTGCCAT CTTCCCCAGC 
CTCGATGAGA TCATTGAAAT GCACTATAAC TTCTACGAGA ACCTGAAAAA GCTGCGGCTG 
GATGACAGCT TCATAGTTAA ATCCATCAGC ACCACAGTGC TCAACAGATT CGGTGGTACA 
GAGGGTGAGT GGTTTCAGAA GCTGACAGCC CGGTTCTGCA GTCACCAGTC ATGGGCCCTG 
GATCAGATTA AGGGCAGACA AAGGAAAGAC CCACGCTTCA ATTCCTTTAT ACAGGAGGCA 
GAGAGTAAGC CCCAGTGCCG CAGGCTGCAG CTCAAGGACA TTATTCCTAT AGAGATGCAG 
AGACTGACCA AGTATCCATT GCTGCTGGAG AATATTGCAA AGAACACAGA GAACCCAACA 
GAGAAGGAGA GCATCCAGCA GAGTGCAGAG TGCTGCAGAA AAATCCTCAA CCATGTCAAT 
GAGGAGGTCA AAGTAATGGA GAACCTGTTG ACTGTGAAGG ACTACCAGCG TAGATTGGAC 
ACATCAGGTC TTAAACCCAG TAACGAACTT TATACAGAAT ACAAGAACAT TGACTTGACT 
CAGAAGAAGA TGCTTTATGA AGGCCCACTG ACCTGGAAAG TCACCAAAGA GAAGGCTATT 
GAGGTGCAGT GTGTGTTGCT TGGTGACCTG CTAGTGCTCC TACAGAGGCA AGATGACAAG 
ATGGTTCTCA AATGCCAGAG CAAAAGTACC ATCGCTGTGC AGGAGGGCAA GCAGATGCTG 
AGCCCCATTA TTAAGCTGGA CTCAGTTTTC CTCCGTGAGG TGGCCACAGA TCGAAAGGCT 
TTCTACGTGA TATTTACCTG GGACAGTGGT GCTCAAATCT ATGAACTCGT GGCTCAGTCT 
GTTGGAGAAA TGAAAACTTG GACTGAGGTG ATAAAGTTAG CAGTAGATGA TCTGAAAAAG 
AGTGGTGCAC CTATGAGGCT GCCACTGCCT CCAGGAGGTG GAGGAGCTCC CTTCAGTCCC 
TCTACGCTAA ATGCCCCTGT GAGCCTGACT GAGAATGGAA GTTTGAAAAG CGGCGCTGAT 
CGAGACAAGT ACGGTTTGAA GTCTACGGAT CCCCGGCACA GGCTAATCGA TTTCCTGTCA 
GATAAAGGCT TTGACTTGAT AGGCCACTCC AACAGCATTC AGGAGAAGGT GGCCAGTCGT 
GCTTTGGATG ACGTCATGTC ACTGAAAAGA CTGTTAATAG GCAGCATCAG TCTATCAGAC 
GACTCAAAAC CTGATGAAGA AAATGAAGAG GAACAATCAG AGAGGCCCGA TCAAGAAAGG 
GATTGCTGTC AGTCAACAGA AGAAAATCCG ACCAGAGACG GAGGGGCTGA AGTGGTAAAC 
GTCAACACAT GTAAAAAAGA AGGTGAAGAG ATGAAAGGAG ATGAAGAAAG GAGCATCAGT 
GCTCCCCTGG TTTTATCCCA GGAGAGGCTG GAGGAAGTGT ACAGGAGGCT GCAGAGCCTG 
GAGGAACAGC TAGAGAGACT ACAGAATGTA GAAGAGGAGC ACCACAGGCT GCAGGAGGCT 
CTTTCTGAGT TCTCACTGGA ACGGGGTAAT TTCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_023263658.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023119426.1 Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OAm103088
Clone ID Related Accession (Same CDS sequence)	XM_023263658.1
Accession Version	XM_023263658.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	rho guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376149.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATCA TCGGGGCCGA GGATGAAGAT TTTGAGAATG ATCTGAATGA TGTGATGGAT 
GACCAGTCCC CGCACTTCAA CAGCATAGAG GTGTTGAAGG AGCGGCCGAC CCACCTGCTG 
GTCTTCATGC AACATGTCAT TCTGCAGTTT GACCCTGCTC CCTTGCTGTG TTACCTCCAT 
GCTGACCTCT TCAGGAGCCT CAGTGCCAAA GAGACCAAGA AACAGTTTGT GGAGTTCTAC 
AATACCTTTT TGGACAAGGG TGCAATTCTC AGAGTGACAA TACCACCTTC AATATCATAT 
GAATTGGACC GGACCCGTCC AGATCTGTTC TCAGAAGAGA CCCAAAGGCG TTTTGCTCAG 
GAGGTTCAGA ACCTGCAGAC TGTTGAAGTG GCAAAACAGT TGGAGGATTT CAGGCAGAAG 
AGAAGGATGG GTATGACGCC CAATGAAGCT GAACTAATTG ATGTGGAGAG TCACTATCCC 
ACCGACCGCG TCCCAATGGA AATGAAGGAA AAGTCTGTGG CTGAGAACCT GCTGGAGAAG 
ATGTCTGAGA CACAACCTAC GATTGTCTCG GACGATGAAA AATGCCAATC CATCTTTACT 
GCAGTGGCCT TCTACATGAA GCACCTTGGA GTTAAAACCA GGGCAGTTGA CAGTAAGAAG 
TCTAGAGGAG GCTTCTTCAG GAGTAAACTG GTAAAGAATA AGAAGGATGA ATCTACAAAG 
CCCAAGCCCA GAGGGGTGTT TCCTGGCATC CCAAGCTGGA TTGGAGGCAA CACGGAAAAG 
GAGAAAGTGG CTCTGGATCG TAAAGGCCCT GCTCCTGGCA GAGGCTCCTT GACCGACCCT 
GCAGTCCCTG CCCTCCCTAG CAGAAAGGCT GGTTCCAGTG GAAGCCCTGC CTCACTGCCT 
GGACTAGAGA TTAGTGATGG CTCGGGCAAC AACATCAGCA CTACCAATAG CCCAGAGTCT 
TTACACACTG ATGGTCTTTC AAGCAATCGT TTAGAGCCCC CAGTACTGTC AGACGGGGGT 
GATGTTTCCC CCGTCGGGAT TTCAGGTGGG CTAATCATTG GGGAGCCCAG CTCACCTTGT 
GACACACCCA CAGACGAGAC TGTGGAGAAA GACAGACGGA AGACAAGTAG GAAAGTGGGG 
CGCAGTGAGA GTGCACGTGT GGACCGGCAC TCGTATCGGC GCCGTGGCTC TTCTCGGGCT 
AAACAGTCTC GCTCCCGTAG TGATGTGGAC CTTCAGCCAC CTTCTACCAC GACAACACCA 
ACTGCCCTGC TCACCCCCCA GCAACTCCAT CCTGTTGATG GAACAGTTTT AGTTCCTGAA 
GGACCTGGCC ACTCCCCCAC CTGTCCCTCA CCACAGCTGG AGGAGATGGA GCCACGTCTC 
TTGGAGTTTG AGCAGGACCC ACCCAACTGG AGAGAGCTGG CCTCTCCTGA AGCCTTGTCC 
AGCCTCAGCA AAAAGGAGAC TAAAAGGCAG GAAGTTATTA ATGAGCTGAT TGCTACAGAG 
CATGCCCATG TGAGAATGCT AAGTGTCCTT CAGATGGTCT TCTCCAAACC ATTGGAGAGA 
CAAGAACTCC TGGCCGCTAC TGAGCTGGCT GCCATCTTCC CCAGCCTCGA TGAGATCATT 
GAAATGCACT ATAACTTCTA CGAGAACCTG AAAAAGCTGC GGCTGGATGA CAGCTTCATA 
GTTAAATCCA TCAGCACCAC AGTGCTCAAC AGATTCGGTG GTACAGAGGG TGAGTGGTTT 
CAGAAGCTGA CAGCCCGGTT CTGCAGTCAC CAGTCATGGG CCCTGGATCA GATTAAGGGC 
AGACAAAGGA AAGACCCACG CTTCAATTCC TTTATACAGG AGGCAGAGAG TAAGCCCCAG 
TGCCGCAGGC TGCAGCTCAA GGACATTATT CCTATAGAGA TGCAGAGACT GACCAAGTAT 
CCATTGCTGC TGGAGAATAT TGCAAAGAAC ACAGAGAACC CAACAGAGAA GGAGAGCATC 
CAGCAGAGTG CAGAGTGCTG CAGAAAAATC CTCAACCATG TCAATGAGGA GGTCAAAGTA 
ATGGAGAACC TGTTGACTGT GAAGGACTAC CAGCGTAGAT TGGACACATC AGGTCTTAAA 
CCCAGTAACG AACTTTATAC AGAATACAAG AACATTGACT TGACTCAGAA GAAGATGCTT 
TATGAAGGCC CACTGACCTG GAAAGTCACC AAAGAGAAGG CTATTGAGGT GCAGTGTGTG 
TTGCTTGGTG ACCTGCTAGT GCTCCTACAG AGGCAAGATG ACAAGATGGT TCTCAAATGC 
CAGAGCAAAA GTACCATCGC TGTGCAGGAG GGCAAGCAGA TGCTGAGCCC CATTATTAAG 
CTGGACTCAG TTTTCCTCCG TGAGGTGGCC ACAGATCGAA AGGCTTTCTA CGTGATATTT 
ACCTGGGACA GTGGTGCTCA AATCTATGAA CTCGTGGCTC AGTCTGTTGG AGAAATGAAA 
ACTTGGACTG AGGTGATAAA GTTAGCAGTA GATGATCTGA AAAAGAGTGG TGCACCTATG 
AGGCTGCCAC TGCCTCCAGG AGGTGGAGGA GCTCCCTTCA GTCCCTCTAC GCTAAATGCC 
CCTGTGAGCC TGACTGAGAA TGGAAGTTTG AAAAGCGGCG CTGATCGAGA CAAGTACGGT 
TTGAAGTCTA CGGATCCCCG GCACAGGCTA ATCGATTTCC TGTCAGATAA AGGCTTTGAC 
TTGATAGGCC ACTCCAACAG CATTCAGGAG AAGGTGGCCA GTCGTGCTTT GGATGACGTC 
ATGTCACTGA AAAGACTGTT AATAGGCAGC ATCAGTCTAT CAGACGACTC AAAACCTGAT 
GAAGAAAATG AAGAGGAACA ATCAGAGAGG CCCGATCAAG AAAGGGATTG CTGTCAGTCA 
ACAGAAGAAA ATCCGACCAG AGACGGAGGG GCTGAAGTGG TAAACGTCAA CACATGTAAA 
AAAGAAGGTG AAGAGATGAA AGGAGATGAA GAAAGGAGCA TCAGTGCTCC CCTGGTTTTA 
TCCCAGGAGA GGCTGGAGGA AGTGTACAGG AGGCTGCAGA GCCTGGAGGA ACAGCTAGAG 
AGACTACAGA ATGTAGAAGA GGAGCACCAC AGGCTGCAGG AGGCTCTTTC TGAGTTCTCA 
CTGGAACGGG GTAATTTCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023119426.1
CDS	107..3250
Translation	MSIIGAEDEDFENDLNDVMDDQSPHFNSIEVLKERPTHLLVFMQHVILQFDPAPLLCYLHADLFRSLSAKETKKQFVEFYNTFLDKGAILRVTIPPSISYELDRTRPDLFSEETQRRFAQEVQNLQTVEVAKQLEDFRQKRRMGMTPNEAELIDVESHYPTDRVPMEMKEKSVAENLLEKMSETQPTIVSDDEKCQSIFTAVAFYMKHLGVKTRAVDSKKSRGGFFRSKLVKNKKDESTKPKPRGVFPGIPSWIGGNTEKEKVALDRKGPAPGRGSLTDPAVPALPSRKAGSSGSPASLPGLEISDGSGNNISTTNSPESLHTDGLSSNRLEPPVLSDGGDVSPVGISGGLIIGEPSSPCDTPTDETVEKDRRKTSRKVGRSESARVDRHSYRRRGSSRAKQSRSRSDVDLQPPSTTTTPTALLTPQQLHPVDGTVLVPEGPGHSPTCPSPQLEEMEPRLLEFEQDPPNWRELASPEALSSLSKKETKRQEVINELIATEHAHVRMLSVLQMVFSKPLERQELLAATELAAIFPSLDEIIEMHYNFYENLKKLRLDDSFIVKSISTTVLNRFGGTEGEWFQKLTARFCSHQSWALDQIKGRQRKDPRFNSFIQEAESKPQCRRLQLKDIIPIEMQRLTKYPLLLENIAKNTENPTEKESIQQSAECCRKILNHVNEEVKVMENLLTVKDYQRRLDTSGLKPSNELYTEYKNIDLTQKKMLYEGPLTWKVTKEKAIEVQCVLLGDLLVLLQRQDDKMVLKCQSKSTIAVQEGKQMLSPIIKLDSVFLREVATDRKAFYVIFTWDSGAQIYELVAQSVGEMKTWTEVIKLAVDDLKKSGAPMRLPLPPGGGGAPFSPSTLNAPVSLTENGSLKSGADRDKYGLKSTDPRHRLIDFLSDKGFDLIGHSNSIQEKVASRALDDVMSLKRLLIGSISLSDDSKPDEENEEEQSERPDQERDCCQSTEENPTRDGGAEVVNVNTCKKEGEEMKGDEERSISAPLVLSQERLEEVYRRLQSLEEQLERLQNVEEEHHRLQEALSEFSLERGNFQ

Target ORF information:

RefSeq Version	XM_023263658.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023263658.1

ORF Insert Sequence:

ATGAGCATCA TCGGGGCCGA GGATGAAGAT TTTGAGAATG ATCTGAATGA TGTGATGGAT 
GACCAGTCCC CGCACTTCAA CAGCATAGAG GTGTTGAAGG AGCGGCCGAC CCACCTGCTG 
GTCTTCATGC AACATGTCAT TCTGCAGTTT GACCCTGCTC CCTTGCTGTG TTACCTCCAT 
GCTGACCTCT TCAGGAGCCT CAGTGCCAAA GAGACCAAGA AACAGTTTGT GGAGTTCTAC 
AATACCTTTT TGGACAAGGG TGCAATTCTC AGAGTGACAA TACCACCTTC AATATCATAT 
GAATTGGACC GGACCCGTCC AGATCTGTTC TCAGAAGAGA CCCAAAGGCG TTTTGCTCAG 
GAGGTTCAGA ACCTGCAGAC TGTTGAAGTG GCAAAACAGT TGGAGGATTT CAGGCAGAAG 
AGAAGGATGG GTATGACGCC CAATGAAGCT GAACTAATTG ATGTGGAGAG TCACTATCCC 
ACCGACCGCG TCCCAATGGA AATGAAGGAA AAGTCTGTGG CTGAGAACCT GCTGGAGAAG 
ATGTCTGAGA CACAACCTAC GATTGTCTCG GACGATGAAA AATGCCAATC CATCTTTACT 
GCAGTGGCCT TCTACATGAA GCACCTTGGA GTTAAAACCA GGGCAGTTGA CAGTAAGAAG 
TCTAGAGGAG GCTTCTTCAG GAGTAAACTG GTAAAGAATA AGAAGGATGA ATCTACAAAG 
CCCAAGCCCA GAGGGGTGTT TCCTGGCATC CCAAGCTGGA TTGGAGGCAA CACGGAAAAG 
GAGAAAGTGG CTCTGGATCG TAAAGGCCCT GCTCCTGGCA GAGGCTCCTT GACCGACCCT 
GCAGTCCCTG CCCTCCCTAG CAGAAAGGCT GGTTCCAGTG GAAGCCCTGC CTCACTGCCT 
GGACTAGAGA TTAGTGATGG CTCGGGCAAC AACATCAGCA CTACCAATAG CCCAGAGTCT 
TTACACACTG ATGGTCTTTC AAGCAATCGT TTAGAGCCCC CAGTACTGTC AGACGGGGGT 
GATGTTTCCC CCGTCGGGAT TTCAGGTGGG CTAATCATTG GGGAGCCCAG CTCACCTTGT 
GACACACCCA CAGACGAGAC TGTGGAGAAA GACAGACGGA AGACAAGTAG GAAAGTGGGG 
CGCAGTGAGA GTGCACGTGT GGACCGGCAC TCGTATCGGC GCCGTGGCTC TTCTCGGGCT 
AAACAGTCTC GCTCCCGTAG TGATGTGGAC CTTCAGCCAC CTTCTACCAC GACAACACCA 
ACTGCCCTGC TCACCCCCCA GCAACTCCAT CCTGTTGATG GAACAGTTTT AGTTCCTGAA 
GGACCTGGCC ACTCCCCCAC CTGTCCCTCA CCACAGCTGG AGGAGATGGA GCCACGTCTC 
TTGGAGTTTG AGCAGGACCC ACCCAACTGG AGAGAGCTGG CCTCTCCTGA AGCCTTGTCC 
AGCCTCAGCA AAAAGGAGAC TAAAAGGCAG GAAGTTATTA ATGAGCTGAT TGCTACAGAG 
CATGCCCATG TGAGAATGCT AAGTGTCCTT CAGATGGTCT TCTCCAAACC ATTGGAGAGA 
CAAGAACTCC TGGCCGCTAC TGAGCTGGCT GCCATCTTCC CCAGCCTCGA TGAGATCATT 
GAAATGCACT ATAACTTCTA CGAGAACCTG AAAAAGCTGC GGCTGGATGA CAGCTTCATA 
GTTAAATCCA TCAGCACCAC AGTGCTCAAC AGATTCGGTG GTACAGAGGG TGAGTGGTTT 
CAGAAGCTGA CAGCCCGGTT CTGCAGTCAC CAGTCATGGG CCCTGGATCA GATTAAGGGC 
AGACAAAGGA AAGACCCACG CTTCAATTCC TTTATACAGG AGGCAGAGAG TAAGCCCCAG 
TGCCGCAGGC TGCAGCTCAA GGACATTATT CCTATAGAGA TGCAGAGACT GACCAAGTAT 
CCATTGCTGC TGGAGAATAT TGCAAAGAAC ACAGAGAACC CAACAGAGAA GGAGAGCATC 
CAGCAGAGTG CAGAGTGCTG CAGAAAAATC CTCAACCATG TCAATGAGGA GGTCAAAGTA 
ATGGAGAACC TGTTGACTGT GAAGGACTAC CAGCGTAGAT TGGACACATC AGGTCTTAAA 
CCCAGTAACG AACTTTATAC AGAATACAAG AACATTGACT TGACTCAGAA GAAGATGCTT 
TATGAAGGCC CACTGACCTG GAAAGTCACC AAAGAGAAGG CTATTGAGGT GCAGTGTGTG 
TTGCTTGGTG ACCTGCTAGT GCTCCTACAG AGGCAAGATG ACAAGATGGT TCTCAAATGC 
CAGAGCAAAA GTACCATCGC TGTGCAGGAG GGCAAGCAGA TGCTGAGCCC CATTATTAAG 
CTGGACTCAG TTTTCCTCCG TGAGGTGGCC ACAGATCGAA AGGCTTTCTA CGTGATATTT 
ACCTGGGACA GTGGTGCTCA AATCTATGAA CTCGTGGCTC AGTCTGTTGG AGAAATGAAA 
ACTTGGACTG AGGTGATAAA GTTAGCAGTA GATGATCTGA AAAAGAGTGG TGCACCTATG 
AGGCTGCCAC TGCCTCCAGG AGGTGGAGGA GCTCCCTTCA GTCCCTCTAC GCTAAATGCC 
CCTGTGAGCC TGACTGAGAA TGGAAGTTTG AAAAGCGGCG CTGATCGAGA CAAGTACGGT 
TTGAAGTCTA CGGATCCCCG GCACAGGCTA ATCGATTTCC TGTCAGATAA AGGCTTTGAC 
TTGATAGGCC ACTCCAACAG CATTCAGGAG AAGGTGGCCA GTCGTGCTTT GGATGACGTC 
ATGTCACTGA AAAGACTGTT AATAGGCAGC ATCAGTCTAT CAGACGACTC AAAACCTGAT 
GAAGAAAATG AAGAGGAACA ATCAGAGAGG CCCGATCAAG AAAGGGATTG CTGTCAGTCA 
ACAGAAGAAA ATCCGACCAG AGACGGAGGG GCTGAAGTGG TAAACGTCAA CACATGTAAA 
AAAGAAGGTG AAGAGATGAA AGGAGATGAA GAAAGGAGCA TCAGTGCTCC CCTGGTTTTA 
TCCCAGGAGA GGCTGGAGGA AGTGTACAGG AGGCTGCAGA GCCTGGAGGA ACAGCTAGAG 
AGACTACAGA ATGTAGAAGA GGAGCACCAC AGGCTGCAGG AGGCTCTTTC TGAGTTCTCA 
CTGGAACGGG GTAATTTCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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arhgef1 cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム