EMG1 cDNA ORF clone, Alligator sinensis(Chinese alligator)

Gene
Clones
gRNAs

Summary

Gene Symbol	EMG1
Entrez Gene ID	102376748
Full Name	EMG1, N1-specific pseudouridine methyltransferase
Gene Type	protein-coding
Organism	Alligator sinensis(Chinese alligator)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025193501.1	XP_025049286.1	ribosomal RNA small subunit methyltransferase NEP1
XM_006037049.2	XP_006037111.1	ribosomal RNA small subunit methyltransferase NEP1

Pathways from BioSystems
Gene Ontology

The following EMG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EMG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAl30759	XM_006037049.2 Latest version!	Alligator sinensis EMG1 N1-specific pseudouridine methyltransferase (EMG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EMG1 ( XM_006037049.2 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_006037111.1 Alligator sinensis EMG1 N1-specific pseudouridine methyltransferase (EMG1), mRNA.

Price & Availability↑

CloneID	OAl30759
Clone ID Related Accession (Same CDS sequence)	XM_006037049.2
Accession Version	XM_006037049.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-29
Organism	Alligator sinensis(Chinese alligator)
Product	ribosomal RNA small subunit methyltransferase NEP1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005843058.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 30, 2015 this sequence version replaced XM_006037049.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 12% of CDS bases ##RefSeq-Attributes-END##

ATGGCGGCGC CCACGCGGCG GCACGATACG GACGCGGCTC CGGCAGCGTG CGGGGGGCCG 
GGGCGGGGTG GGTTGGGGCG GGGCCGGCGG CCACTGACGC AGTTTCCCGC ACAGGCCGGC 
AGGACGTACG AGCTGCTGAA CTGCGACAAG CACAAGGCGC TGCTGGCGCG GAGCGGCCGC 
GACCCGGGCG CCGTCAGGCC TGACATCACC CATCAGAGCC TCCTGATGCT CATGGACAGT 
CCCCTGAACC GGGCTGGCTT GCTGCAGGTG TACATCCACA CACAGAAGAA TGTGCTGATT 
GAAGTAAACC CCCAGACAAG GATTCCCCGC ACATTTGACC GTTTTTGTGG TCTGATGGTT 
CAGTTGCTGC ATAAACTCAG TGTCCGATCA GCTGATGGAC CACAGAAACT TTTGAAGGTA 
ATTAAAAATC CAGTGACTGA TCATCTTCCT GTGGGCTGCA TGAAGATAGG CACTTCTTTC 
TCAACTCCTG ATGTGACGGA TGCACGCAAA CTAGTTCCTG AAGAACAGCC TGTTGCTGTT 
GTTGTGGGAG CTTTTGCCCA TGGCTCGCTG AATGTTGACT ATACGGAGAA GATGGTTTCT 
ATCAGCAACT ATCCTCTTTC TGCTGCATTG ACTTGTGCCA AGATCACTAC AGCCTTTGAG 
GAGGTTTGGG GAGTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006037111.1
CDS	1..681
Translation	MAAPTRRHDTDAAPAACGGPGRGGLGRGRRPLTQFPAQAGRTYELLNCDKHKALLARSGRDPGAVRPDITHQSLLMLMDSPLNRAGLLQVYIHTQKNVLIEVNPQTRIPRTFDRFCGLMVQLLHKLSVRSADGPQKLLKVIKNPVTDHLPVGCMKIGTSFSTPDVTDARKLVPEEQPVAVVVGAFAHGSLNVDYTEKMVSISNYPLSAALTCAKITTAFEEVWGVV

Target ORF information:

RefSeq Version	XM_006037049.2
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis EMG1 N1-specific pseudouridine methyltransferase (EMG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006037049.2

ORF Insert Sequence:

ATGGCGGCGC CCACGCGGCG GCACGATACG GACGCGGCTC CGGCAGCGTG CGGGGGGCCG 
GGGCGGGGTG GGTTGGGGCG GGGCCGGCGG CCACTGACGC AGTTTCCCGC ACAGGCCGGC 
AGGACGTACG AGCTGCTGAA CTGCGACAAG CACAAGGCGC TGCTGGCGCG GAGCGGCCGC 
GACCCGGGCG CCGTCAGGCC TGACATCACC CATCAGAGCC TCCTGATGCT CATGGACAGT 
CCCCTGAACC GGGCTGGCTT GCTGCAGGTG TACATCCACA CACAGAAGAA TGTGCTGATT 
GAAGTAAACC CCCAGACAAG GATTCCCCGC ACATTTGACC GTTTTTGTGG TCTGATGGTT 
CAGTTGCTGC ATAAACTCAG TGTCCGATCA GCTGATGGAC CACAGAAACT TTTGAAGGTA 
ATTAAAAATC CAGTGACTGA TCATCTTCCT GTGGGCTGCA TGAAGATAGG CACTTCTTTC 
TCAACTCCTG ATGTGACGGA TGCACGCAAA CTAGTTCCTG AAGAACAGCC TGTTGCTGTT 
GTTGTGGGAG CTTTTGCCCA TGGCTCGCTG AATGTTGACT ATACGGAGAA GATGGTTTCT 
ATCAGCAACT ATCCTCTTTC TGCTGCATTG ACTTGTGCCA AGATCACTAC AGCCTTTGAG 
GAGGTTTGGG GAGTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.