MARCH6 cDNA ORF clone, Alligator sinensis(Chinese alligator)

Gene
Clones
gRNAs

Summary

Gene Symbol	MARCH6
Entrez Gene ID	102371021
Full Name	membrane associated ring-CH-type finger 6
Gene Type	protein-coding
Organism	Alligator sinensis(Chinese alligator)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014517642.1	XP_014373128.1	E3 ubiquitin-protein ligase MARCH6
XM_025198302.1	XP_025054087.1	E3 ubiquitin-protein ligase MARCH6

Pathways from BioSystems
Gene Ontology

The following MARCH6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MARCH6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAl13838	XM_014517642.1 Latest version!	Alligator sinensis membrane-associated ring finger (C3HC4) 6, E3 ubiquitin protein ligase (MARCH6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAl83198	XM_025198302.1 Latest version!	Alligator sinensis membrane associated ring-CH-type finger 6 (MARCH6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MARCH6 ( XM_014517642.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_014373128.1 Alligator sinensis membrane-associated ring finger (C3HC4) 6, E3 ubiquitin protein ligase (MARCH6), mRNA.

Price & Availability↑

CloneID	OAl13838
Clone ID Related Accession (Same CDS sequence)	XM_014517642.1
Accession Version	XM_014517642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-29
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase MARCH6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841919.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 8% of CDS bases ##RefSeq-Attributes-END##

ATGAATGAAG CTGCCAAGAA GGTTGTGATC ATGGACATAA CCATCCCCTT TGAGAACTGG 
CACCAAGCTT TCACCAATGC CCAGGCTCAC AAGCGAGAGA AGTATGCCCT GCTGGCCAAC 
ATCCTGAGGG GCCGTGGCTA CGAAGTGACA GTCAACATAC TCATCGTGGG AATGATTGGA 
GCCTGGGACC CAAGCAACGA GTGTCTTGCG TGCCTGCTGT GTCTCCTGCC GCTACGCCAA 
GCTGATACGC TGCCTCATGA TATATGCAGG GTATGTCGGT CTGAGGGAAC ACCTGAGAAA 
CCGCTCTATC ATCCATGTGT GTGTACTGGC AGTATTAAAT TTATTCATCA AGAATGCTTA 
GTTCAGTGGC TTAAACACAG CAGAAAAGAA TACTGTGAAT TATGTAAGCA CAGATTTGCT 
TTCACACCAA TTTATTCCCC AGATATGCCT TCACGGCTTC CAATCCAAGA CATCTTTGCT 
GGGCTGGTTA CAAGTATTGG TACAGCAATA CGATACTGGT TTCACTATAC ACTTGTGGCC 
TTTGCGTGGT TGGGAGTTGT ACCTCTTACA GCATGCCGTA TCTACAAGTG CTTGTTTACT 
GGCTCTGTGA GCTCACTACT GACACTGCCG TTAGATATGT TGTCAACGGA AAATTTATTG 
GCAGACTGTT TGCAGGGTTG TTTTGTGGTG ACTTGCACTT TGTGTGCATT TATCAGTCTA 
GTGTGGTTAC GTGAACAGAT TGTCCATGGT GGAGCACCAC AGTGGCTGGA AAAAAATCAG 
CAAACGCCAC TCAATGGTGT TGGCCAACAG AATGAGGCTC AGGGTGCAGG AAATGTTGGA 
GCTGATAATG CTGCACTGGA CCAACCAGCC AATCCACCTG CTGAGAATGC AGTTGTAGGA 
GAAAACCCTG AAATCCAGGA AGAACAAGTA GATGAGGAAG AGGAAGACAA TGAAGATGAA 
GAAGATGCTG TAGCAGAAGA TGCAGCAGAT GCAAACAATG GAGCACAAGA TGACATGAAT 
TGGAATGCTT TGGAATGGGA TCGAGCTGCA GAAGAACTTA CCTGGGAGAG AATGCTTGGG 
CTTGATGGAT CTCTGGTTTT CTTAGAACAT GTTTTCTGGG TGGTATCATT AAACACATTG 
TTCATACTTG TGTTTGCGTT TTGTCCTTAC CATATTGGTC ACTTCTCGGT GGTTGGCCTG 
GGTTTTGAGG AATATGTTCA AGCATCTCAC TTTGAAGGCC TGATCACAAC AATAGTTGGC 
TATGTGTTGC TAGCAATAAC TCTAATTGTT TGTCATGGTT TGGCAGCACT AGTCAAGTTC 
CAGAGATCCC GTCGCTTATT AGGAGTCTGC TATATCGTTG TTAAGGTTTC CTTGTTAGTG 
GTGGTAGAAA TTGGTGTGTT TCCCCTCATC TGCGGCTGGT GGTTGGATAT ATGCTCCTTG 
GAAATGTTTG ATGCTACTTT GAAAGACAGA GAGTTAAGCT TCCAGTCTGC CCCTGGTACC 
ACAATGTTCC TGCACTGGCT AGTGGGAATG GTTTATGTCT TTTACTTTGC ATCCTTCATT 
CTGTTACTGA GAGAGGTACT GAGACCTGGT GTCCTTTGGT TCCTGAGGAA TTTGAATGAT 
CCAGATTTTA ATCCAGTTCA GGAAATGATT CACTTACCAA TTTATAGACA TCTTAGGAGG 
TTTATTTTAT CAGTGATTGT CTTTGGGTCA ATTGTCCTGC TCATGCTTTG GCTTCCTATC 
CGTATTATTA AGTATCTGCT GCCTAATTTT CTTCCGTATA ATGTCATGCT CTACAGTGAT 
GCTCCTGTGA GTGAACTTTC CCTGGAGCTA CTTTTGCTTC AGGTGGTGCT GCCAGCTTTG 
CTTGAACAAG GACACACAAG ACAGTGGTTG AAAGGTCTTG TACGAGCATG GACTGTTACT 
GCTGGTTACT TACTGGACCT TCATTCCTAC TTACTTGGTG ACCAGGAAGA AAATGAAAAC 
AATGCAAACC AGCAGCCTAA TAACCAGCAT GCTCGCAATA ACAATGCCAT TCCAGTTGTA 
GGAGAAGGTC TTCATGCGGC CCATCAAGCC ATACTTCAAC AAGGAGGACC TGTGGGTTTC 
CAGCCTTATC GTAGGCCTTT AAAATTCCCG CTCAGGATAT TTCTGTTGAT TATTTTCATG 
TGTATAACAT TACTGATTGC CAGTCTTATC TGCCTTACAT TACCAGTGTT TGCTGGGAGA 
TGGCTAATGT CATTTTGGAC GGGAACTGCC AAAATCCATG AACTGTACAC AGCTGCTTGT 
GGTCTTTATG TCTGCTGGCT AACCATTCGG GCAGCAACAG TACTAGTTGC ATGGATGCCA 
CAGGGTCGCA GAGTGATTTT TCAGAAGGTT AAAGAGTGGT CTCTCATGAT AATGAAGACG 
CTAATAGTGG CTGTGCTGCT AGCTGGTGTG GTTCCACTTT TGCTTGGTCT TCTGTTTGAA 
CTGGTAATTG TTGCGCCTTT GAGAGTCCCT TTGGATCAGA CACCTTTGTT CTACCCATGG 
CAGGACTGGG CTCTTGGAGT TCTGCATGCC AAAATAATTG CTGCCATAAC ACTGATGGGT 
CCTCAGTGGT GGCTAAAAAC AGTTATTGAA CAGGTTTATG CAAATGGGAT TCGTAACATT 
GATTTACATT TTATAATTCG TAAACTGGCA GCACCCGTCA TCTCTGTTCT GTTACTTTCT 
CTTTGTGTAC CTTACATCAT TGCTTCTGGT GTTGTACCTT TATTAGGTGT TACTGCTGAA 
ATGCAGAACC TGGTACAGCG TCGGATTTAT CCTTTCCTAC TTATGGTGGT GGTTTTGATG 
GGAATCCTAT CCTTCCAGGT CCGCCAGTTT AAGCGCCTTT ATGAACATAT TAAAAATGAC 
AAGTACCTTG TGGGGCAACG GCTTGTGAAT TATGAGCGAA AAGCTGGTAA ACAAGGCACA 
TCAACAACAC CACCTCAATC TTCACAAGAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014373128.1
CDS	1..2973
Translation	MNEAAKKVVIMDITIPFENWHQAFTNAQAHKREKYALLANILRGRGYEVTVNILIVGMIGAWDPSNECLACLLCLLPLRQADTLPHDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPQWLEKNQQTPLNGVGQQNEAQGAGNVGADNAALDQPANPPAENAVVGENPEIQEEQVDEEEEDNEDEEDAVAEDAADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSVVGLGFEEYVQASHFEGLITTIVGYVLLAITLIVCHGLAALVKFQRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKYLLPNFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEENENNANQQPNNQHARNNNAIPVVGEGLHAAHQAILQQGGPVGFQPYRRPLKFPLRIFLLIIFMCITLLIASLICLTLPVFAGRWLMSFWTGTAKIHELYTAACGLYVCWLTIRAATVLVAWMPQGRRVIFQKVKEWSLMIMKTLIVAVLLAGVVPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHFIIRKLAAPVISVLLLSLCVPYIIASGVVPLLGVTAEMQNLVQRRIYPFLLMVVVLMGILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKAGKQGTSTTPPQSSQE

Target ORF information:

RefSeq Version	XM_014517642.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis membrane-associated ring finger (C3HC4) 6, E3 ubiquitin protein ligase (MARCH6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014517642.1

ORF Insert Sequence:

ATGAATGAAG CTGCCAAGAA GGTTGTGATC ATGGACATAA CCATCCCCTT TGAGAACTGG 
CACCAAGCTT TCACCAATGC CCAGGCTCAC AAGCGAGAGA AGTATGCCCT GCTGGCCAAC 
ATCCTGAGGG GCCGTGGCTA CGAAGTGACA GTCAACATAC TCATCGTGGG AATGATTGGA 
GCCTGGGACC CAAGCAACGA GTGTCTTGCG TGCCTGCTGT GTCTCCTGCC GCTACGCCAA 
GCTGATACGC TGCCTCATGA TATATGCAGG GTATGTCGGT CTGAGGGAAC ACCTGAGAAA 
CCGCTCTATC ATCCATGTGT GTGTACTGGC AGTATTAAAT TTATTCATCA AGAATGCTTA 
GTTCAGTGGC TTAAACACAG CAGAAAAGAA TACTGTGAAT TATGTAAGCA CAGATTTGCT 
TTCACACCAA TTTATTCCCC AGATATGCCT TCACGGCTTC CAATCCAAGA CATCTTTGCT 
GGGCTGGTTA CAAGTATTGG TACAGCAATA CGATACTGGT TTCACTATAC ACTTGTGGCC 
TTTGCGTGGT TGGGAGTTGT ACCTCTTACA GCATGCCGTA TCTACAAGTG CTTGTTTACT 
GGCTCTGTGA GCTCACTACT GACACTGCCG TTAGATATGT TGTCAACGGA AAATTTATTG 
GCAGACTGTT TGCAGGGTTG TTTTGTGGTG ACTTGCACTT TGTGTGCATT TATCAGTCTA 
GTGTGGTTAC GTGAACAGAT TGTCCATGGT GGAGCACCAC AGTGGCTGGA AAAAAATCAG 
CAAACGCCAC TCAATGGTGT TGGCCAACAG AATGAGGCTC AGGGTGCAGG AAATGTTGGA 
GCTGATAATG CTGCACTGGA CCAACCAGCC AATCCACCTG CTGAGAATGC AGTTGTAGGA 
GAAAACCCTG AAATCCAGGA AGAACAAGTA GATGAGGAAG AGGAAGACAA TGAAGATGAA 
GAAGATGCTG TAGCAGAAGA TGCAGCAGAT GCAAACAATG GAGCACAAGA TGACATGAAT 
TGGAATGCTT TGGAATGGGA TCGAGCTGCA GAAGAACTTA CCTGGGAGAG AATGCTTGGG 
CTTGATGGAT CTCTGGTTTT CTTAGAACAT GTTTTCTGGG TGGTATCATT AAACACATTG 
TTCATACTTG TGTTTGCGTT TTGTCCTTAC CATATTGGTC ACTTCTCGGT GGTTGGCCTG 
GGTTTTGAGG AATATGTTCA AGCATCTCAC TTTGAAGGCC TGATCACAAC AATAGTTGGC 
TATGTGTTGC TAGCAATAAC TCTAATTGTT TGTCATGGTT TGGCAGCACT AGTCAAGTTC 
CAGAGATCCC GTCGCTTATT AGGAGTCTGC TATATCGTTG TTAAGGTTTC CTTGTTAGTG 
GTGGTAGAAA TTGGTGTGTT TCCCCTCATC TGCGGCTGGT GGTTGGATAT ATGCTCCTTG 
GAAATGTTTG ATGCTACTTT GAAAGACAGA GAGTTAAGCT TCCAGTCTGC CCCTGGTACC 
ACAATGTTCC TGCACTGGCT AGTGGGAATG GTTTATGTCT TTTACTTTGC ATCCTTCATT 
CTGTTACTGA GAGAGGTACT GAGACCTGGT GTCCTTTGGT TCCTGAGGAA TTTGAATGAT 
CCAGATTTTA ATCCAGTTCA GGAAATGATT CACTTACCAA TTTATAGACA TCTTAGGAGG 
TTTATTTTAT CAGTGATTGT CTTTGGGTCA ATTGTCCTGC TCATGCTTTG GCTTCCTATC 
CGTATTATTA AGTATCTGCT GCCTAATTTT CTTCCGTATA ATGTCATGCT CTACAGTGAT 
GCTCCTGTGA GTGAACTTTC CCTGGAGCTA CTTTTGCTTC AGGTGGTGCT GCCAGCTTTG 
CTTGAACAAG GACACACAAG ACAGTGGTTG AAAGGTCTTG TACGAGCATG GACTGTTACT 
GCTGGTTACT TACTGGACCT TCATTCCTAC TTACTTGGTG ACCAGGAAGA AAATGAAAAC 
AATGCAAACC AGCAGCCTAA TAACCAGCAT GCTCGCAATA ACAATGCCAT TCCAGTTGTA 
GGAGAAGGTC TTCATGCGGC CCATCAAGCC ATACTTCAAC AAGGAGGACC TGTGGGTTTC 
CAGCCTTATC GTAGGCCTTT AAAATTCCCG CTCAGGATAT TTCTGTTGAT TATTTTCATG 
TGTATAACAT TACTGATTGC CAGTCTTATC TGCCTTACAT TACCAGTGTT TGCTGGGAGA 
TGGCTAATGT CATTTTGGAC GGGAACTGCC AAAATCCATG AACTGTACAC AGCTGCTTGT 
GGTCTTTATG TCTGCTGGCT AACCATTCGG GCAGCAACAG TACTAGTTGC ATGGATGCCA 
CAGGGTCGCA GAGTGATTTT TCAGAAGGTT AAAGAGTGGT CTCTCATGAT AATGAAGACG 
CTAATAGTGG CTGTGCTGCT AGCTGGTGTG GTTCCACTTT TGCTTGGTCT TCTGTTTGAA 
CTGGTAATTG TTGCGCCTTT GAGAGTCCCT TTGGATCAGA CACCTTTGTT CTACCCATGG 
CAGGACTGGG CTCTTGGAGT TCTGCATGCC AAAATAATTG CTGCCATAAC ACTGATGGGT 
CCTCAGTGGT GGCTAAAAAC AGTTATTGAA CAGGTTTATG CAAATGGGAT TCGTAACATT 
GATTTACATT TTATAATTCG TAAACTGGCA GCACCCGTCA TCTCTGTTCT GTTACTTTCT 
CTTTGTGTAC CTTACATCAT TGCTTCTGGT GTTGTACCTT TATTAGGTGT TACTGCTGAA 
ATGCAGAACC TGGTACAGCG TCGGATTTAT CCTTTCCTAC TTATGGTGGT GGTTTTGATG 
GGAATCCTAT CCTTCCAGGT CCGCCAGTTT AAGCGCCTTT ATGAACATAT TAAAAATGAC 
AAGTACCTTG TGGGGCAACG GCTTGTGAAT TATGAGCGAA AAGCTGGTAA ACAAGGCACA 
TCAACAACAC CACCTCAATC TTCACAAGAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MARCH6 ( XM_025198302.1 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_025054087.1 Alligator sinensis membrane associated ring-CH-type finger 6 (MARCH6), mRNA.

Price & Availability↑

CloneID	OAl83198
Clone ID Related Accession (Same CDS sequence)	XM_025198302.1
Accession Version	XM_025198302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	E3 ubiquitin-protein ligase MARCH6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005841919.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 7% of CDS bases ##RefSeq-Attributes-END##

ATGAATGAAG CTGCCAAGAA GGTTGTGATC ATGGACATAA CCATCCCCTT TGAGAACTGG 
CACCAAGCTT TCACCAATGC CCAGGCTCAC AAGCGAGAGA AGTATGCCCT GCTGGCCAAC 
ATCCTGAGGG GCCGTGGCTA CGAAGTGACA GTCAACATAC TCATCGTGGG AATGATTGGA 
GCCTGGGACC CAAGCAACGA GTGTCTTGCT GTAATAAGGT TCTTGATTTG GCCGGCAGAT 
ATATGCAGGG TATGTCGGTC TGAGGGAACA CCTGAGAAAC CGCTCTATCA TCCATGTGTG 
TGTACTGGCA GTATTAAATT TATTCATCAA GAATGCTTAG TTCAGTGGCT TAAACACAGC 
AGAAAAGAAT ACTGTGAATT ATGTAAGCAC AGATTTGCTT TCACACCAAT TTATTCCCCA 
GATATGCCTT CACGGCTTCC AATCCAAGAC ATCTTTGCTG GGCTGGTTAC AAGTATTGGT 
ACAGCAATAC GATACTGGTT TCACTATACA CTTGTGGCCT TTGCGTGGTT GGGAGTTGTA 
CCTCTTACAG CATGCCGTAT CTACAAGTGC TTGTTTACTG GCTCTGTGAG CTCACTACTG 
ACACTGCCGT TAGATATGTT GTCAACGGAA AATTTATTGG CAGACTGTTT GCAGGGTTGT 
TTTGTGGTGA CTTGCACTTT GTGTGCATTT ATCAGTCTAG TGTGGTTACG TGAACAGATT 
GTCCATGGTG GAGCACCACA GTGGCTGGAA AAAAATCAGC AAACGCCACT CAATGGTGTT 
GGCCAACAGA ATGAGGCTCA GGGTGCAGGA AATGTTGGAG CTGATAATGC TGCACTGGAC 
CAACCAGCCA ATCCACCTGC TGAGAATGCA GTTGTAGGAG AAAACCCTGA AATCCAGGAA 
GAACAAGTAG ATGAGGAAGA GGAAGACAAT GAAGATGAAG AAGATGCTGT AGCAGAAGAT 
GCAGCAGATG CAAACAATGG AGCACAAGAT GACATGAATT GGAATGCTTT GGAATGGGAT 
CGAGCTGCAG AAGAACTTAC CTGGGAGAGA ATGCTTGGGC TTGATGGATC TCTGGTTTTC 
TTAGAACATG TTTTCTGGGT GGTATCATTA AACACATTGT TCATACTTGT GTTTGCGTTT 
TGTCCTTACC ATATTGGTCA CTTCTCGGTG GTTGGCCTGG GTTTTGAGGA ATATGTTCAA 
GCATCTCACT TTGAAGGCCT GATCACAACA ATAGTTGGCT ATGTGTTGCT AGCAATAACT 
CTAATTGTTT GTCATGGTTT GGCAGCACTA GTCAAGTTCC AGAGATCCCG TCGCTTATTA 
GGAGTCTGCT ATATCGTTGT TAAGGTTTCC TTGTTAGTGG TGGTAGAAAT TGGTGTGTTT 
CCCCTCATCT GCGGCTGGTG GTTGGATATA TGCTCCTTGG AAATGTTTGA TGCTACTTTG 
AAAGACAGAG AGTTAAGCTT CCAGTCTGCC CCTGGTACCA CAATGTTCCT GCACTGGCTA 
GTGGGAATGG TTTATGTCTT TTACTTTGCA TCCTTCATTC TGTTACTGAG AGAGGTACTG 
AGACCTGGTG TCCTTTGGTT CCTGAGGAAT TTGAATGATC CAGATTTTAA TCCAGTTCAG 
GAAATGATTC ACTTACCAAT TTATAGACAT CTTAGGAGGT TTATTTTATC AGTGATTGTC 
TTTGGGTCAA TTGTCCTGCT CATGCTTTGG CTTCCTATCC GTATTATTAA GTATCTGCTG 
CCTAATTTTC TTCCGTATAA TGTCATGCTC TACAGTGATG CTCCTGTGAG TGAACTTTCC 
CTGGAGCTAC TTTTGCTTCA GGTGGTGCTG CCAGCTTTGC TTGAACAAGG ACACACAAGA 
CAGTGGTTGA AAGGTCTTGT ACGAGCATGG ACTGTTACTG CTGGTTACTT ACTGGACCTT 
CATTCCTACT TACTTGGTGA CCAGGAAGAA AATGAAAACA ATGCAAACCA GCAGCCTAAT 
AACCAGCATG CTCGCAATAA CAATGCCATT CCAGTTGTAG GAGAAGGTCT TCATGCGGCC 
CATCAAGCCA TACTTCAACA AGGAGGACCT GTGGGTTTCC AGCCTTATCG TAGGCCTTTA 
AAATTCCCGC TCAGGATATT TCTGTTGATT ATTTTCATGT GTATAACATT ACTGATTGCC 
AGTCTTATCT GCCTTACATT ACCAGTGTTT GCTGGGAGAT GGCTAATGTC ATTTTGGACG 
GGAACTGCCA AAATCCATGA ACTGTACACA GCTGCTTGTG GTCTTTATGT CTGCTGGCTA 
ACCATTCGGG CAGCAACAGT ACTAGTTGCA TGGATGCCAC AGGGTCGCAG AGTGATTTTT 
CAGAAGGTTA AAGAGTGGTC TCTCATGATA ATGAAGACGC TAATAGTGGC TGTGCTGCTA 
GCTGGTGTGG TTCCACTTTT GCTTGGTCTT CTGTTTGAAC TGGTAATTGT TGCGCCTTTG 
AGAGTCCCTT TGGATCAGAC ACCTTTGTTC TACCCATGGC AGGACTGGGC TCTTGGAGTT 
CTGCATGCCA AAATAATTGC TGCCATAACA CTGATGGGTC CTCAGTGGTG GCTAAAAACA 
GTTATTGAAC AGGTTTATGC AAATGGGATT CGTAACATTG ATTTACATTT TATAATTCGT 
AAACTGGCAG CACCCGTCAT CTCTGTTCTG TTACTTTCTC TTTGTGTACC TTACATCATT 
GCTTCTGGTG TTGTACCTTT ATTAGGTGTT ACTGCTGAAA TGCAGAACCT GGTACAGCGT 
CGGATTTATC CTTTCCTACT TATGGTGGTG GTTTTGATGG GAATCCTATC CTTCCAGGTC 
CGCCAGTTTA AGCGCCTTTA TGAACATATT AAAAATGACA AGTACCTTGT GGGGCAACGG 
CTTGTGAATT ATGAGCGAAA AGCTGGTAAA CAAGGCACAT CAACAACACC ACCTCAATCT 
TCACAAGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025054087.1
CDS	1..2952
Translation	MNEAAKKVVIMDITIPFENWHQAFTNAQAHKREKYALLANILRGRGYEVTVNILIVGMIGAWDPSNECLAVIRFLIWPADICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPQWLEKNQQTPLNGVGQQNEAQGAGNVGADNAALDQPANPPAENAVVGENPEIQEEQVDEEEEDNEDEEDAVAEDAADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSVVGLGFEEYVQASHFEGLITTIVGYVLLAITLIVCHGLAALVKFQRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKYLLPNFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEENENNANQQPNNQHARNNNAIPVVGEGLHAAHQAILQQGGPVGFQPYRRPLKFPLRIFLLIIFMCITLLIASLICLTLPVFAGRWLMSFWTGTAKIHELYTAACGLYVCWLTIRAATVLVAWMPQGRRVIFQKVKEWSLMIMKTLIVAVLLAGVVPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHFIIRKLAAPVISVLLLSLCVPYIIASGVVPLLGVTAEMQNLVQRRIYPFLLMVVVLMGILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKAGKQGTSTTPPQSSQE

Target ORF information:

RefSeq Version	XM_025198302.1
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis membrane associated ring-CH-type finger 6 (MARCH6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025198302.1

ORF Insert Sequence:

ATGAATGAAG CTGCCAAGAA GGTTGTGATC ATGGACATAA CCATCCCCTT TGAGAACTGG 
CACCAAGCTT TCACCAATGC CCAGGCTCAC AAGCGAGAGA AGTATGCCCT GCTGGCCAAC 
ATCCTGAGGG GCCGTGGCTA CGAAGTGACA GTCAACATAC TCATCGTGGG AATGATTGGA 
GCCTGGGACC CAAGCAACGA GTGTCTTGCT GTAATAAGGT TCTTGATTTG GCCGGCAGAT 
ATATGCAGGG TATGTCGGTC TGAGGGAACA CCTGAGAAAC CGCTCTATCA TCCATGTGTG 
TGTACTGGCA GTATTAAATT TATTCATCAA GAATGCTTAG TTCAGTGGCT TAAACACAGC 
AGAAAAGAAT ACTGTGAATT ATGTAAGCAC AGATTTGCTT TCACACCAAT TTATTCCCCA 
GATATGCCTT CACGGCTTCC AATCCAAGAC ATCTTTGCTG GGCTGGTTAC AAGTATTGGT 
ACAGCAATAC GATACTGGTT TCACTATACA CTTGTGGCCT TTGCGTGGTT GGGAGTTGTA 
CCTCTTACAG CATGCCGTAT CTACAAGTGC TTGTTTACTG GCTCTGTGAG CTCACTACTG 
ACACTGCCGT TAGATATGTT GTCAACGGAA AATTTATTGG CAGACTGTTT GCAGGGTTGT 
TTTGTGGTGA CTTGCACTTT GTGTGCATTT ATCAGTCTAG TGTGGTTACG TGAACAGATT 
GTCCATGGTG GAGCACCACA GTGGCTGGAA AAAAATCAGC AAACGCCACT CAATGGTGTT 
GGCCAACAGA ATGAGGCTCA GGGTGCAGGA AATGTTGGAG CTGATAATGC TGCACTGGAC 
CAACCAGCCA ATCCACCTGC TGAGAATGCA GTTGTAGGAG AAAACCCTGA AATCCAGGAA 
GAACAAGTAG ATGAGGAAGA GGAAGACAAT GAAGATGAAG AAGATGCTGT AGCAGAAGAT 
GCAGCAGATG CAAACAATGG AGCACAAGAT GACATGAATT GGAATGCTTT GGAATGGGAT 
CGAGCTGCAG AAGAACTTAC CTGGGAGAGA ATGCTTGGGC TTGATGGATC TCTGGTTTTC 
TTAGAACATG TTTTCTGGGT GGTATCATTA AACACATTGT TCATACTTGT GTTTGCGTTT 
TGTCCTTACC ATATTGGTCA CTTCTCGGTG GTTGGCCTGG GTTTTGAGGA ATATGTTCAA 
GCATCTCACT TTGAAGGCCT GATCACAACA ATAGTTGGCT ATGTGTTGCT AGCAATAACT 
CTAATTGTTT GTCATGGTTT GGCAGCACTA GTCAAGTTCC AGAGATCCCG TCGCTTATTA 
GGAGTCTGCT ATATCGTTGT TAAGGTTTCC TTGTTAGTGG TGGTAGAAAT TGGTGTGTTT 
CCCCTCATCT GCGGCTGGTG GTTGGATATA TGCTCCTTGG AAATGTTTGA TGCTACTTTG 
AAAGACAGAG AGTTAAGCTT CCAGTCTGCC CCTGGTACCA CAATGTTCCT GCACTGGCTA 
GTGGGAATGG TTTATGTCTT TTACTTTGCA TCCTTCATTC TGTTACTGAG AGAGGTACTG 
AGACCTGGTG TCCTTTGGTT CCTGAGGAAT TTGAATGATC CAGATTTTAA TCCAGTTCAG 
GAAATGATTC ACTTACCAAT TTATAGACAT CTTAGGAGGT TTATTTTATC AGTGATTGTC 
TTTGGGTCAA TTGTCCTGCT CATGCTTTGG CTTCCTATCC GTATTATTAA GTATCTGCTG 
CCTAATTTTC TTCCGTATAA TGTCATGCTC TACAGTGATG CTCCTGTGAG TGAACTTTCC 
CTGGAGCTAC TTTTGCTTCA GGTGGTGCTG CCAGCTTTGC TTGAACAAGG ACACACAAGA 
CAGTGGTTGA AAGGTCTTGT ACGAGCATGG ACTGTTACTG CTGGTTACTT ACTGGACCTT 
CATTCCTACT TACTTGGTGA CCAGGAAGAA AATGAAAACA ATGCAAACCA GCAGCCTAAT 
AACCAGCATG CTCGCAATAA CAATGCCATT CCAGTTGTAG GAGAAGGTCT TCATGCGGCC 
CATCAAGCCA TACTTCAACA AGGAGGACCT GTGGGTTTCC AGCCTTATCG TAGGCCTTTA 
AAATTCCCGC TCAGGATATT TCTGTTGATT ATTTTCATGT GTATAACATT ACTGATTGCC 
AGTCTTATCT GCCTTACATT ACCAGTGTTT GCTGGGAGAT GGCTAATGTC ATTTTGGACG 
GGAACTGCCA AAATCCATGA ACTGTACACA GCTGCTTGTG GTCTTTATGT CTGCTGGCTA 
ACCATTCGGG CAGCAACAGT ACTAGTTGCA TGGATGCCAC AGGGTCGCAG AGTGATTTTT 
CAGAAGGTTA AAGAGTGGTC TCTCATGATA ATGAAGACGC TAATAGTGGC TGTGCTGCTA 
GCTGGTGTGG TTCCACTTTT GCTTGGTCTT CTGTTTGAAC TGGTAATTGT TGCGCCTTTG 
AGAGTCCCTT TGGATCAGAC ACCTTTGTTC TACCCATGGC AGGACTGGGC TCTTGGAGTT 
CTGCATGCCA AAATAATTGC TGCCATAACA CTGATGGGTC CTCAGTGGTG GCTAAAAACA 
GTTATTGAAC AGGTTTATGC AAATGGGATT CGTAACATTG ATTTACATTT TATAATTCGT 
AAACTGGCAG CACCCGTCAT CTCTGTTCTG TTACTTTCTC TTTGTGTACC TTACATCATT 
GCTTCTGGTG TTGTACCTTT ATTAGGTGTT ACTGCTGAAA TGCAGAACCT GGTACAGCGT 
CGGATTTATC CTTTCCTACT TATGGTGGTG GTTTTGATGG GAATCCTATC CTTCCAGGTC 
CGCCAGTTTA AGCGCCTTTA TGAACATATT AAAAATGACA AGTACCTTGT GGGGCAACGG 
CTTGTGAATT ATGAGCGAAA AGCTGGTAAA CAAGGCACAT CAACAACACC ACCTCAATCT 
TCACAAGAAT AG