NEDD4 cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEDD4
Entrez Gene ID	100464254
Full Name	neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Ailuropoda melanoleuca(giant panda)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011229546.2	XP_011227848.1	E3 ubiquitin-protein ligase NEDD4 isoform X2
XM_002922948.3	XP_002922994.1	E3 ubiquitin-protein ligase NEDD4 isoform X1

Pathways from BioSystems
Gene Ontology

The following NEDD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEDD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc10246	XM_002922948.3 Latest version!	Ailuropoda melanoleuca neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAc18474	XM_011229546.2 Latest version!	Ailuropoda melanoleuca neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEDD4 ( XM_002922948.3 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_002922994.1 Ailuropoda melanoleuca neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc10246
Clone ID Related Accession (Same CDS sequence)	XM_002922948.3
Accession Version	XM_002922948.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	E3 ubiquitin-protein ligase NEDD4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217929.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 28, 2016 this sequence version replaced XM_002922948.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTT GCGCGGTGGA GGTGTTCGGG CTCCCCGAGG ACGAGGAAAA TTCACGAATT 
GTGAGGGTCA AAGTCATAGC TGGAATAGGC CTTGCCAAAA AGGATATCTT GGGAGCTAGT 
GATCCTTATG TGAGAGTGAC GTTGTATGAC CCAATGAATG GAATCTTTAC AAGTGTGCAA 
ACGAAAACCC TTAAAAAGAC TTTGAATCCA AAATGGAATG AAGAAATATT ATTCAGAGTC 
CATCCTCAGC AGCACCGGCT TCTTTTTGAA GTATTTGATG AAAATCGATT GACAAGAGAT 
GATTTCCTAG GTCAAGTGGA TGTTCCTCTT TATCCATTAC CGACAGAAAA TCCAAGACTG 
GAGAGACCAT ATACGTTTAA GGATTTTGTT CTTCACCCAA GAAGTCACAA ATCAAGAGTT 
AAAGGTTATC TGAGATTAAA AATGACTTAC TTACCTAAAA CCAGTGGCTC AGAAGAAGAT 
AACACAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTTG TTTTGGACCA ACCAGATGCT 
GCTTGCCATT TGCAGCAGCA ACAAGAACCT TCTCCTCTAC CTCCAGGATG GGAAGAGAGG 
CAGGATATTC TTGGAAGGAC CTACTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
AGACCAACTC CCCAGGACAA CCTAACGGAT GCTGAGAACG GCAACCTGCA GGCACAGCGT 
GCGTTTACCA CCAGGCGGCA GATATCTGAG GAGACAGAAA GTGTGGATAA TCGAGAGTCT 
TCCGAGAACT GGGAAATTAT AAGAGAAGAG GAAGCCACCA TGTATAGCAA TCAGGCCTTC 
CCATCACCTC CAGCATCAAA TAACTTGAAT GTTCAGACTC ATCTTGCAGA AGAATTGAAT 
GCCAGACTCA CAGTTTGTAG AAATTCAGCC TCCGGCCAGC CAGTATCCAG CTCAAATCAT 
TCCAGCAGAA GAGGCAGCTT ACAAGCCTAT ACTTTTGAGG AACAGCCTAC ACTTCCTGTG 
CTTTTGCCTA CTTCATCTGG ATTACCACCA GGTTGGGAAG AAAAGCAAGA TGAAAGAGGA 
AGACCATATT ACGTAGATCA CAATTCCAGA ACTACCACCT GGACGAAGCC CATAGTACAG 
GCCACAGCGG AGACCAGTCA ACTGCAGTCA GGCCAGAGTT CGGGGAGCCC TCACCCACAG 
GTCCCCGCGA GTGACTCAGC GCAGCAGGTG ACCCAGCCGT CTGAAGTGGA GCAAGGATTC 
CTCCCTAAAG GCTGGGAAGT CCGGCACGCA CCAAATGGGA GGCCTTTCTT TATTGACCAC 
AACACCAAAA CCACTACCTG GGAAGATCCA AGACTGAAAA TTCCAGCTCA TCTGAGAGGA 
AAGACATCAC TTGATTCTTC CAATGACCTG GGGCCTTTAC CTCCAGGATG GGAAGAAAGA 
ACTCATACAG ATGGAAGAAT CTTCTACATA AATCACAATA TAAAAAGAAC ACAATGGGAA 
GACCCTCGGT TGCAGAATGT AGCAATAACT GGACCAGCAG TGCCCTACTC CAGGGATTAC 
AAAAGAAAGT ATGAGTTCTT CCGACGAAAG TTGAAGAAGC AGAATGACAT TCCAAACAAA 
TTTGAAATGA AACTTCGCCG TGCAACTGTC CTTGAGGACT CTTACCGGAG AATAATGGGT 
GTTAAGAGAG CGGATTTCCT CAAGGCTCGA CTGTGGATCG AGTTCGATGG TGAAAAGGGA 
CTGGATTATG GAGGAGTCGC CAGAGAATGG TTCTTCCTGA TCTCAAAGGA AATGTTTAAC 
CCTTATTATG GGCTGTTTGA ATATTCTGCT ACGGATAATT ACACCCTGCA GATAAATCCA 
AACTCCGGAT TGTGTAATGA GGATCACCTC TCTTACTTCA AGTTTATTGG CCGGGTAGCT 
GGAATGGCAG TTTATCACGG CAAACTGCTG GATGGTTTTT TCATCCGCCC ATTTTACAAG 
ATGATGCTAC ACAAACCAAT AACCCTTCAT GATATGGAAT CAGTGGATAG TGAATATTAC 
AATTCACTAC GATGGATTCT TGAAAATGAC CCGACGGAAT TGGACCTCAG GTTTGTCATA 
GATGAAGAGC TTTTTGGGCA GACACATCAA CATGAGTTGA AAATCGGTGG ATCAGAAATA 
GTTGTCACCA ATAAGAACAA AAAGGAATAT ATTTATCTTG TAATACAGTG GCGATTTGTA 
AACCGAATCC AGAAGCAAAT GGCTGCTTTT AAAGAGGGAT TTTTTGAACT AATACCACAG 
GATCTCATCA AAATTTTCGA TGAAAACGAA TTAGAGCTTC TTATGTGTGG ATTGGGAGAC 
GTCGATGTGA ATGACTGGAG AGAACACACA AAGTATAAGA ACGGCTACAG TGTGAATCAT 
CAAGTCATAC AGTGGTTTTG GAAGGCTGTT CTAATGATGG ATTCAGAAAA AAGAATAAGG 
TTACTTCAGT TTGTCACTGG CACCTCTCGG GTGCCCATGA ATGGATTTGC TGAACTATAT 
GGCTCAAATG GACCACAGTC ATTTACAGTT GAACAGTGGG GTACGCCAGA AAAGCTGCCA 
AGAGCCCACA CATGCTTCAA TCGCTTGGAC TTGCCACCTT ATGAATCATT TGAAGAATTG 
TGGGATAAAC TTCAGATGGC AATTGAGAAC ACTCAGGGCT TTGATGGCGT CGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002922994.1
CDS	84..2780
Translation	MATCAVEVFGLPEDEENSRIVRVKVIAGIGLAKKDILGASDPYVRVTLYDPMNGIFTSVQTKTLKKTLNPKWNEEILFRVHPQQHRLLFEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEEDNTEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNLQAQRAFTTRRQISEETESVDNRESSENWEIIREEEATMYSNQAFPSPPASNNLNVQTHLAEELNARLTVCRNSASGQPVSSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRPYYVDHNSRTTTWTKPIVQATAETSQLQSGQSSGSPHPQVPASDSAQQVTQPSEVEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDSSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLQNVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFVIDEELFGQTHQHELKIGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSVNHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	XM_002922948.3
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002922948.3

ORF Insert Sequence:

ATGGCAACTT GCGCGGTGGA GGTGTTCGGG CTCCCCGAGG ACGAGGAAAA TTCACGAATT 
GTGAGGGTCA AAGTCATAGC TGGAATAGGC CTTGCCAAAA AGGATATCTT GGGAGCTAGT 
GATCCTTATG TGAGAGTGAC GTTGTATGAC CCAATGAATG GAATCTTTAC AAGTGTGCAA 
ACGAAAACCC TTAAAAAGAC TTTGAATCCA AAATGGAATG AAGAAATATT ATTCAGAGTC 
CATCCTCAGC AGCACCGGCT TCTTTTTGAA GTATTTGATG AAAATCGATT GACAAGAGAT 
GATTTCCTAG GTCAAGTGGA TGTTCCTCTT TATCCATTAC CGACAGAAAA TCCAAGACTG 
GAGAGACCAT ATACGTTTAA GGATTTTGTT CTTCACCCAA GAAGTCACAA ATCAAGAGTT 
AAAGGTTATC TGAGATTAAA AATGACTTAC TTACCTAAAA CCAGTGGCTC AGAAGAAGAT 
AACACAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTTG TTTTGGACCA ACCAGATGCT 
GCTTGCCATT TGCAGCAGCA ACAAGAACCT TCTCCTCTAC CTCCAGGATG GGAAGAGAGG 
CAGGATATTC TTGGAAGGAC CTACTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
AGACCAACTC CCCAGGACAA CCTAACGGAT GCTGAGAACG GCAACCTGCA GGCACAGCGT 
GCGTTTACCA CCAGGCGGCA GATATCTGAG GAGACAGAAA GTGTGGATAA TCGAGAGTCT 
TCCGAGAACT GGGAAATTAT AAGAGAAGAG GAAGCCACCA TGTATAGCAA TCAGGCCTTC 
CCATCACCTC CAGCATCAAA TAACTTGAAT GTTCAGACTC ATCTTGCAGA AGAATTGAAT 
GCCAGACTCA CAGTTTGTAG AAATTCAGCC TCCGGCCAGC CAGTATCCAG CTCAAATCAT 
TCCAGCAGAA GAGGCAGCTT ACAAGCCTAT ACTTTTGAGG AACAGCCTAC ACTTCCTGTG 
CTTTTGCCTA CTTCATCTGG ATTACCACCA GGTTGGGAAG AAAAGCAAGA TGAAAGAGGA 
AGACCATATT ACGTAGATCA CAATTCCAGA ACTACCACCT GGACGAAGCC CATAGTACAG 
GCCACAGCGG AGACCAGTCA ACTGCAGTCA GGCCAGAGTT CGGGGAGCCC TCACCCACAG 
GTCCCCGCGA GTGACTCAGC GCAGCAGGTG ACCCAGCCGT CTGAAGTGGA GCAAGGATTC 
CTCCCTAAAG GCTGGGAAGT CCGGCACGCA CCAAATGGGA GGCCTTTCTT TATTGACCAC 
AACACCAAAA CCACTACCTG GGAAGATCCA AGACTGAAAA TTCCAGCTCA TCTGAGAGGA 
AAGACATCAC TTGATTCTTC CAATGACCTG GGGCCTTTAC CTCCAGGATG GGAAGAAAGA 
ACTCATACAG ATGGAAGAAT CTTCTACATA AATCACAATA TAAAAAGAAC ACAATGGGAA 
GACCCTCGGT TGCAGAATGT AGCAATAACT GGACCAGCAG TGCCCTACTC CAGGGATTAC 
AAAAGAAAGT ATGAGTTCTT CCGACGAAAG TTGAAGAAGC AGAATGACAT TCCAAACAAA 
TTTGAAATGA AACTTCGCCG TGCAACTGTC CTTGAGGACT CTTACCGGAG AATAATGGGT 
GTTAAGAGAG CGGATTTCCT CAAGGCTCGA CTGTGGATCG AGTTCGATGG TGAAAAGGGA 
CTGGATTATG GAGGAGTCGC CAGAGAATGG TTCTTCCTGA TCTCAAAGGA AATGTTTAAC 
CCTTATTATG GGCTGTTTGA ATATTCTGCT ACGGATAATT ACACCCTGCA GATAAATCCA 
AACTCCGGAT TGTGTAATGA GGATCACCTC TCTTACTTCA AGTTTATTGG CCGGGTAGCT 
GGAATGGCAG TTTATCACGG CAAACTGCTG GATGGTTTTT TCATCCGCCC ATTTTACAAG 
ATGATGCTAC ACAAACCAAT AACCCTTCAT GATATGGAAT CAGTGGATAG TGAATATTAC 
AATTCACTAC GATGGATTCT TGAAAATGAC CCGACGGAAT TGGACCTCAG GTTTGTCATA 
GATGAAGAGC TTTTTGGGCA GACACATCAA CATGAGTTGA AAATCGGTGG ATCAGAAATA 
GTTGTCACCA ATAAGAACAA AAAGGAATAT ATTTATCTTG TAATACAGTG GCGATTTGTA 
AACCGAATCC AGAAGCAAAT GGCTGCTTTT AAAGAGGGAT TTTTTGAACT AATACCACAG 
GATCTCATCA AAATTTTCGA TGAAAACGAA TTAGAGCTTC TTATGTGTGG ATTGGGAGAC 
GTCGATGTGA ATGACTGGAG AGAACACACA AAGTATAAGA ACGGCTACAG TGTGAATCAT 
CAAGTCATAC AGTGGTTTTG GAAGGCTGTT CTAATGATGG ATTCAGAAAA AAGAATAAGG 
TTACTTCAGT TTGTCACTGG CACCTCTCGG GTGCCCATGA ATGGATTTGC TGAACTATAT 
GGCTCAAATG GACCACAGTC ATTTACAGTT GAACAGTGGG GTACGCCAGA AAAGCTGCCA 
AGAGCCCACA CATGCTTCAA TCGCTTGGAC TTGCCACCTT ATGAATCATT TGAAGAATTG 
TGGGATAAAC TTCAGATGGC AATTGAGAAC ACTCAGGGCT TTGATGGCGT CGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4 ( XM_011229546.2 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_011227848.1 Ailuropoda melanoleuca neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAc18474
Clone ID Related Accession (Same CDS sequence)	XM_011229546.2
Accession Version	XM_011229546.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	E3 ubiquitin-protein ligase NEDD4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217929.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 28, 2016 this sequence version replaced XM_011229546.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA TCCTCTGTCA 
GAAAGCTCCG GAGATGATTT GGAGAGCAAT GTTCCCATGT GCTTCAAAAG ACCAACCCGG 
ATTTCCACGT CTAACGTTGT TCAAATGAAG CTGACTCCTA GACAGACTGC ACTAGCTCCG 
TTAATACAGG AAAACGTTAA GTCTCAGGAA AGATCATCTG TTCTTTCACC TGAAAATGTT 
AATAAAAAGA GCAGCTGTCT GCAGATTTCA CTACAGCCAA CCAGGTACAG TGGATGTCTT 
CAGTCTAGCC ATGTCTTAGC TGACGGGGAG GATGCTTCCT TTATGTGTGT CTTGAAGGAC 
GGCGTTTACA ACAATGCTGG GGCTGATAAT GAGCTGAATG CTGTGAGTGA GGGTAACCTC 
GTGAGCAGTT CTGCCATTCG CAGTGATAGC CTTAGTAACT TTTCCACCAG TGAGAATGGG 
TCTTACAGCA GCAACGGTAG TGATTATGGA TCATGCACAA GTATCACGAG TGGTGGTTCC 
TACACAAACA GTATCATCAG TGACAGCAGT GGTTATACTT TTCCACCAAC CGATGATAAT 
TTTTTGGGTG GAAATTTACC TTCTGACAGC ACCTCCAACA GGAGTGTGCC AAACAGGAAT 
ACTGCTCCGT GTGAAGTTTT TTCAAGAAGT CCAAGCACCG TTCCTTTTGT CCAGGATGAC 
TTGGAGCGTG GACTAGAAGT TATGAAGTTG CCAGTGAGCA GGAACTCGAA AATTCCACTG 
AAACGTTGTT CATCTTTAGT CATTTTTCCT AGGAGTCCTT CAGATACCCC ACCAACTTCT 
CCAACAAGTA CAGATATTCT TCCAAGCAAA GGATCCTATC AAACCTCACA CCAGTTTATT 
ATTTCTCCTA GTGAACTTGC CCACACTGAA GACGGTACTA GTGCTAAGGG ATTTCTTTCC 
ACAGCTGTCA ATGGACTTCG CTTATCTAAA ACAGCCTGTC CTCCTGGAGA AGTAAGAGAC 
ATACGCCCGC TTCACAGGAA GGGCTCGTTA CAAAAGAAAA TTGCTATTGC AAATAATAGT 
CTGAATCAGA CTGTCTGTGA AAAGTCATCT GAAGGATATT CTTGTGTGTC AGTGCATTTC 
ACCCAACAAA AAGCAACTAC ATTAGATTGT GAAACAGCAA ATGGTGATTG TAAACCAGGG 
ATGTCAGAAA GGAAGCTTAA CTCTGATTCA GAGTGCATGA AGCTTATGCA AAGGACATCG 
GCATGTTTAC CATCCTCTCA AAATACAGAT TACCAAATAA ATATCAATGG ACAGTTGGAA 
AGACCAAATT TACAGATAAA CAAAAACCAT GCTATTTTAC AAAGAAGTAT TTCATTGGGA 
GGAACTTATC CAAATGTTTC CTGTTTATCC AGCCTTAAGC ACAATTGTTC TAAGGGGGGA 
CCATCACAGT TACTCATAAA GTTTGCATCT GGAAATGAAG ATAAAGTTGA TAACTTATCA 
AGAGACAGTA ACAGAGATTT TGCAAATGAA CTATCTAATT CTTTAAAGCA TTCTTGTAAG 
ACAAGAGATG ATTTCCTAGG TCAAGTGGAT GTTCCTCTTT ATCCATTACC GACAGAAAAT 
CCAAGACTGG AGAGACCATA TACGTTTAAG GATTTTGTTC TTCACCCAAG AAGTCACAAA 
TCAAGAGTTA AAGGTTATCT GAGATTAAAA ATGACTTACT TACCTAAAAC CAGTGGCTCA 
GAAGAAGATA ACACAGAACA GGCTGAGGAA TTAGAGCCTG GCTGGGTTGT TTTGGACCAA 
CCAGATGCTG CTTGCCATTT GCAGCAGCAA CAAGAACCTT CTCCTCTACC TCCAGGATGG 
GAAGAGAGGC AGGATATTCT TGGAAGGACC TACTATGTAA ACCATGAATC TAGAAGAACA 
CAGTGGAAAA GACCAACTCC CCAGGACAAC CTAACGGATG CTGAGAACGG CAACCTGCAG 
GCACAGCGTG CGTTTACCAC CAGGCGGCAG ATATCTGAGG AGACAGAAAG TGTGGATAAT 
CGAGAGTCTT CCGAGAACTG GGAAATTATA AGAGAAGAGG AAGCCACCAT GTATAGCAAT 
CAGGCCTTCC CATCACCTCC AGCATCAAAT AACTTGAATG TTCAGACTCA TCTTGCAGAA 
GAATTGAATG CCAGACTCAC AGTTTGTAGA AATTCAGCCT CCGGCCAGCC AGTATCCAGC 
TCAAATCATT CCAGCAGAAG AGGCAGCTTA CAAGCCTATA CTTTTGAGGA ACAGCCTACA 
CTTCCTGTGC TTTTGCCTAC TTCATCTGGA TTACCACCAG GTTGGGAAGA AAAGCAAGAT 
GAAAGAGGAA GACCATATTA CGTAGATCAC AATTCCAGAA CTACCACCTG GACGAAGCCC 
ATAGTACAGG CCACAGCGGA GACCAGTCAA CTGCAGTCAG GCCAGAGTTC GGGGAGCCCT 
CACCCACAGG TCCCCGCGAG TGACTCAGCG CAGCAGGTGA CCCAGCCGTC TGAAGTGGAG 
CAAGGATTCC TCCCTAAAGG CTGGGAAGTC CGGCACGCAC CAAATGGGAG GCCTTTCTTT 
ATTGACCACA ACACCAAAAC CACTACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT 
CTGAGAGGAA AGACATCACT TGATTCTTCC AATGACCTGG GGCCTTTACC TCCAGGATGG 
GAAGAAAGAA CTCATACAGA TGGAAGAATC TTCTACATAA ATCACAATAT AAAAAGAACA 
CAATGGGAAG ACCCTCGGTT GCAGAATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC 
AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGACGAAAGT TGAAGAAGCA GAATGACATT 
CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTCC TTGAGGACTC TTACCGGAGA 
ATAATGGGTG TTAAGAGAGC GGATTTCCTC AAGGCTCGAC TGTGGATCGA GTTCGATGGT 
GAAAAGGGAC TGGATTATGG AGGAGTCGCC AGAGAATGGT TCTTCCTGAT CTCAAAGGAA 
ATGTTTAACC CTTATTATGG GCTGTTTGAA TATTCTGCTA CGGATAATTA CACCCTGCAG 
ATAAATCCAA ACTCCGGATT GTGTAATGAG GATCACCTCT CTTACTTCAA GTTTATTGGC 
CGGGTAGCTG GAATGGCAGT TTATCACGGC AAACTGCTGG ATGGTTTTTT CATCCGCCCA 
TTTTACAAGA TGATGCTACA CAAACCAATA ACCCTTCATG ATATGGAATC AGTGGATAGT 
GAATATTACA ATTCACTACG ATGGATTCTT GAAAATGACC CGACGGAATT GGACCTCAGG 
TTTGTCATAG ATGAAGAGCT TTTTGGGCAG ACACATCAAC ATGAGTTGAA AATCGGTGGA 
TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG 
CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGATT TTTTGAACTA 
ATACCACAGG ATCTCATCAA AATTTTCGAT GAAAACGAAT TAGAGCTTCT TATGTGTGGA 
TTGGGAGACG TCGATGTGAA TGACTGGAGA GAACACACAA AGTATAAGAA CGGCTACAGT 
GTGAATCATC AAGTCATACA GTGGTTTTGG AAGGCTGTTC TAATGATGGA TTCAGAAAAA 
AGAATAAGGT TACTTCAGTT TGTCACTGGC ACCTCTCGGG TGCCCATGAA TGGATTTGCT 
GAACTATATG GCTCAAATGG ACCACAGTCA TTTACAGTTG AACAGTGGGG TACGCCAGAA 
AAGCTGCCAA GAGCCCACAC ATGCTTCAAT CGCTTGGACT TGCCACCTTA TGAATCATTT 
GAAGAATTGT GGGATAAACT TCAGATGGCA ATTGAGAACA CTCAGGGCTT TGATGGCGTC 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011227848.1
CDS	276..4241
Translation	MAQSLRLHFAARRSNTYPLSESSGDDLESNVPMCFKRPTRISTSNVVQMKLTPRQTALAPLIQENVKSQERSSVLSPENVNKKSSCLQISLQPTRYSGCLQSSHVLADGEDASFMCVLKDGVYNNAGADNELNAVSEGNLVSSSAIRSDSLSNFSTSENGSYSSNGSDYGSCTSITSGGSYTNSIISDSSGYTFPPTDDNFLGGNLPSDSTSNRSVPNRNTAPCEVFSRSPSTVPFVQDDLERGLEVMKLPVSRNSKIPLKRCSSLVIFPRSPSDTPPTSPTSTDILPSKGSYQTSHQFIISPSELAHTEDGTSAKGFLSTAVNGLRLSKTACPPGEVRDIRPLHRKGSLQKKIAIANNSLNQTVCEKSSEGYSCVSVHFTQQKATTLDCETANGDCKPGMSERKLNSDSECMKLMQRTSACLPSSQNTDYQININGQLERPNLQINKNHAILQRSISLGGTYPNVSCLSSLKHNCSKGGPSQLLIKFASGNEDKVDNLSRDSNRDFANELSNSLKHSCKTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEEDNTEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNLQAQRAFTTRRQISEETESVDNRESSENWEIIREEEATMYSNQAFPSPPASNNLNVQTHLAEELNARLTVCRNSASGQPVSSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRPYYVDHNSRTTTWTKPIVQATAETSQLQSGQSSGSPHPQVPASDSAQQVTQPSEVEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDSSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLQNVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFVIDEELFGQTHQHELKIGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSVNHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	XM_011229546.2
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011229546.2

ORF Insert Sequence:

ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA TCCTCTGTCA 
GAAAGCTCCG GAGATGATTT GGAGAGCAAT GTTCCCATGT GCTTCAAAAG ACCAACCCGG 
ATTTCCACGT CTAACGTTGT TCAAATGAAG CTGACTCCTA GACAGACTGC ACTAGCTCCG 
TTAATACAGG AAAACGTTAA GTCTCAGGAA AGATCATCTG TTCTTTCACC TGAAAATGTT 
AATAAAAAGA GCAGCTGTCT GCAGATTTCA CTACAGCCAA CCAGGTACAG TGGATGTCTT 
CAGTCTAGCC ATGTCTTAGC TGACGGGGAG GATGCTTCCT TTATGTGTGT CTTGAAGGAC 
GGCGTTTACA ACAATGCTGG GGCTGATAAT GAGCTGAATG CTGTGAGTGA GGGTAACCTC 
GTGAGCAGTT CTGCCATTCG CAGTGATAGC CTTAGTAACT TTTCCACCAG TGAGAATGGG 
TCTTACAGCA GCAACGGTAG TGATTATGGA TCATGCACAA GTATCACGAG TGGTGGTTCC 
TACACAAACA GTATCATCAG TGACAGCAGT GGTTATACTT TTCCACCAAC CGATGATAAT 
TTTTTGGGTG GAAATTTACC TTCTGACAGC ACCTCCAACA GGAGTGTGCC AAACAGGAAT 
ACTGCTCCGT GTGAAGTTTT TTCAAGAAGT CCAAGCACCG TTCCTTTTGT CCAGGATGAC 
TTGGAGCGTG GACTAGAAGT TATGAAGTTG CCAGTGAGCA GGAACTCGAA AATTCCACTG 
AAACGTTGTT CATCTTTAGT CATTTTTCCT AGGAGTCCTT CAGATACCCC ACCAACTTCT 
CCAACAAGTA CAGATATTCT TCCAAGCAAA GGATCCTATC AAACCTCACA CCAGTTTATT 
ATTTCTCCTA GTGAACTTGC CCACACTGAA GACGGTACTA GTGCTAAGGG ATTTCTTTCC 
ACAGCTGTCA ATGGACTTCG CTTATCTAAA ACAGCCTGTC CTCCTGGAGA AGTAAGAGAC 
ATACGCCCGC TTCACAGGAA GGGCTCGTTA CAAAAGAAAA TTGCTATTGC AAATAATAGT 
CTGAATCAGA CTGTCTGTGA AAAGTCATCT GAAGGATATT CTTGTGTGTC AGTGCATTTC 
ACCCAACAAA AAGCAACTAC ATTAGATTGT GAAACAGCAA ATGGTGATTG TAAACCAGGG 
ATGTCAGAAA GGAAGCTTAA CTCTGATTCA GAGTGCATGA AGCTTATGCA AAGGACATCG 
GCATGTTTAC CATCCTCTCA AAATACAGAT TACCAAATAA ATATCAATGG ACAGTTGGAA 
AGACCAAATT TACAGATAAA CAAAAACCAT GCTATTTTAC AAAGAAGTAT TTCATTGGGA 
GGAACTTATC CAAATGTTTC CTGTTTATCC AGCCTTAAGC ACAATTGTTC TAAGGGGGGA 
CCATCACAGT TACTCATAAA GTTTGCATCT GGAAATGAAG ATAAAGTTGA TAACTTATCA 
AGAGACAGTA ACAGAGATTT TGCAAATGAA CTATCTAATT CTTTAAAGCA TTCTTGTAAG 
ACAAGAGATG ATTTCCTAGG TCAAGTGGAT GTTCCTCTTT ATCCATTACC GACAGAAAAT 
CCAAGACTGG AGAGACCATA TACGTTTAAG GATTTTGTTC TTCACCCAAG AAGTCACAAA 
TCAAGAGTTA AAGGTTATCT GAGATTAAAA ATGACTTACT TACCTAAAAC CAGTGGCTCA 
GAAGAAGATA ACACAGAACA GGCTGAGGAA TTAGAGCCTG GCTGGGTTGT TTTGGACCAA 
CCAGATGCTG CTTGCCATTT GCAGCAGCAA CAAGAACCTT CTCCTCTACC TCCAGGATGG 
GAAGAGAGGC AGGATATTCT TGGAAGGACC TACTATGTAA ACCATGAATC TAGAAGAACA 
CAGTGGAAAA GACCAACTCC CCAGGACAAC CTAACGGATG CTGAGAACGG CAACCTGCAG 
GCACAGCGTG CGTTTACCAC CAGGCGGCAG ATATCTGAGG AGACAGAAAG TGTGGATAAT 
CGAGAGTCTT CCGAGAACTG GGAAATTATA AGAGAAGAGG AAGCCACCAT GTATAGCAAT 
CAGGCCTTCC CATCACCTCC AGCATCAAAT AACTTGAATG TTCAGACTCA TCTTGCAGAA 
GAATTGAATG CCAGACTCAC AGTTTGTAGA AATTCAGCCT CCGGCCAGCC AGTATCCAGC 
TCAAATCATT CCAGCAGAAG AGGCAGCTTA CAAGCCTATA CTTTTGAGGA ACAGCCTACA 
CTTCCTGTGC TTTTGCCTAC TTCATCTGGA TTACCACCAG GTTGGGAAGA AAAGCAAGAT 
GAAAGAGGAA GACCATATTA CGTAGATCAC AATTCCAGAA CTACCACCTG GACGAAGCCC 
ATAGTACAGG CCACAGCGGA GACCAGTCAA CTGCAGTCAG GCCAGAGTTC GGGGAGCCCT 
CACCCACAGG TCCCCGCGAG TGACTCAGCG CAGCAGGTGA CCCAGCCGTC TGAAGTGGAG 
CAAGGATTCC TCCCTAAAGG CTGGGAAGTC CGGCACGCAC CAAATGGGAG GCCTTTCTTT 
ATTGACCACA ACACCAAAAC CACTACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT 
CTGAGAGGAA AGACATCACT TGATTCTTCC AATGACCTGG GGCCTTTACC TCCAGGATGG 
GAAGAAAGAA CTCATACAGA TGGAAGAATC TTCTACATAA ATCACAATAT AAAAAGAACA 
CAATGGGAAG ACCCTCGGTT GCAGAATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC 
AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGACGAAAGT TGAAGAAGCA GAATGACATT 
CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTCC TTGAGGACTC TTACCGGAGA 
ATAATGGGTG TTAAGAGAGC GGATTTCCTC AAGGCTCGAC TGTGGATCGA GTTCGATGGT 
GAAAAGGGAC TGGATTATGG AGGAGTCGCC AGAGAATGGT TCTTCCTGAT CTCAAAGGAA 
ATGTTTAACC CTTATTATGG GCTGTTTGAA TATTCTGCTA CGGATAATTA CACCCTGCAG 
ATAAATCCAA ACTCCGGATT GTGTAATGAG GATCACCTCT CTTACTTCAA GTTTATTGGC 
CGGGTAGCTG GAATGGCAGT TTATCACGGC AAACTGCTGG ATGGTTTTTT CATCCGCCCA 
TTTTACAAGA TGATGCTACA CAAACCAATA ACCCTTCATG ATATGGAATC AGTGGATAGT 
GAATATTACA ATTCACTACG ATGGATTCTT GAAAATGACC CGACGGAATT GGACCTCAGG 
TTTGTCATAG ATGAAGAGCT TTTTGGGCAG ACACATCAAC ATGAGTTGAA AATCGGTGGA 
TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG 
CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGATT TTTTGAACTA 
ATACCACAGG ATCTCATCAA AATTTTCGAT GAAAACGAAT TAGAGCTTCT TATGTGTGGA 
TTGGGAGACG TCGATGTGAA TGACTGGAGA GAACACACAA AGTATAAGAA CGGCTACAGT 
GTGAATCATC AAGTCATACA GTGGTTTTGG AAGGCTGTTC TAATGATGGA TTCAGAAAAA 
AGAATAAGGT TACTTCAGTT TGTCACTGGC ACCTCTCGGG TGCCCATGAA TGGATTTGCT 
GAACTATATG GCTCAAATGG ACCACAGTCA TTTACAGTTG AACAGTGGGG TACGCCAGAA 
AAGCTGCCAA GAGCCCACAC ATGCTTCAAT CGCTTGGACT TGCCACCTTA TGAATCATTT 
GAAGAATTGT GGGATAAACT TCAGATGGCA ATTGAGAACA CTCAGGGCTT TGATGGCGTC 
GATTAG