AAEL_AAEL007683 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	AAEL_AAEL007683
Entrez Gene ID	5569510
Full Name	DNA topoisomerase 3-alpha
Synonyms	AAEL007683
Gene Type	protein-coding
Organism	Aedes aegypti(Yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001658545.2	XP_001658595.1	DNA topoisomerase 3-alpha
XM_001658545.1	XP_001658595.1	AAEL007683-PA

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AAEL_AAEL007683 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AAEL_AAEL007683 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe05200	XM_001658545.2 Latest version!	Aedes aegypti DNA topoisomerase 3-alpha (LOC5569510), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OAe05200	XM_001658545.1	Aedes aegypti AAEL007683-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AAEL_AAEL007683 ( XM_001658545.2 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_001658595.1 Aedes aegypti DNA topoisomerase 3-alpha (LOC5569510), mRNA.

Price & Availability↑

CloneID	OAe05200
Clone ID Related Accession (Same CDS sequence)	XM_001658545.2 , XM_001658545.1
Accession Version	XM_001658545.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-19
Organism	Aedes aegypti(yellow fever mosquito)
Product	DNA topoisomerase 3-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 20, 2017 this sequence version replaced XM_001658545.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACGTT CGTTATTCAA CGTTAGAATA AGAAGATTTT GCTCAGATAC TAAACCGGGC 
AATGCCGCAG TCATGAAGTA CCTCAACGTA GCAGAGAAAA ACGATGCGGC CAAAAACATT 
GCCGGTTTAC TTTCGCGAGG GTCTTCCCAA CGGCGCGAAG GCTACTCCCC GTACAACAAG 
ATCTACGAGT TCAGTTACAA TATCCGGGGC CAGCCGGTCC AGATGGTAAT GACCTCCGTT 
TCGGGCCATT TGCTGAACTA TGAGTTTCTT CCTTCGTTCC GAGGATGGCA CAGTTGTAAT 
CCGGAGCAGT TGTTCGATGC TCCGGTTCGG AAGTCGTGCA CGGAAAACAT GGGCAAAATC 
AAACAGACTT TGGAAAGGGA AGTCCGAGGC TGCCAAACCC TGGTCATTTG GACGGATTGC 
GATCGCGAAG GAGAAAACAT TGGATTCGAG ATAATCGAGG TTTGTCGGGC CATAAAACCT 
GGGATCAGAG TTTTGAGGGC GAAGTTTTCG GAAATAACGG CGGCCTCGGT AAAGCGGGCG 
GTCGAAAATC TGGCCGCACC GGATGAACTG CAGAGTGAGG CCGTAAATGT GCGCAGCGAG 
TTGGATCTTC GGATCGGGGC TGCCTTTACC CGATTCCAGA CACTTCGGCT GCAGAATGTG 
TTTCCGGCGA AGATCGTCAA TAACCTGGTC TCGTACGGAA GTTGCCAGAT TCCGACGCTG 
GGCTTTGTCG CTCACCGATA TAAGGAGATT GAAAAGTTTA TTCCGCAGGA GTTTTGGAAA 
ATCAAGTTAA ATCACACCAT CGATGAGCTG ACGGTGGAGT TCAATTGGGC GAGGAATCGT 
CTTTTCGATA AGCAATGCTG TGAGGCGTAT TTGATGCTTT GTCAATCGGA ACCACTGGTT 
AAAATTGTTA GCGTTACGAA CAAACCGAAA AATAAGTGGC GTCCAACGCC ATTGGATACG 
GTCGAATTGG AAAAACTTGG ATCGAGAAAG CTGAAAATCA ATGCCAAGCA GACAATGACG 
ATAGCGGAAA AACTGTACAC CCAAGGTATC ATCAGTTATC CTCGTACCGA AACGAACATG 
TTTACGAACG ATATGAAACT AGCGCCACTG GTGGAGATGC AAACTGATAA TCCCGAGTGG 
GGTGGATTTG CACAGAAAGT GCTTCAATGG GGACCAAATC CTCGAAATGG CAAAAAATCC 
GATCAAGCGC ATCCTCCCAT TCATCCAACT AAGTATGTCT CCAACCTAAC TGGTGATGAG 
AAGCGGATAT ACGAACTAGT GGTTCGCCAC TTCCTGGCCT GCATAAGTAA GGATGCTGTC 
GGATCGGAAA CTGTCGTCAA TGCTGTTCTG GCTGATGAGG AATTCACGGC TTCCGGATTG 
TGCATTCTTG AGAGGAACTA TCTAGAAGTC TATCCGTATG ATCGATGGAA TTCAAGAGAG 
ATCCACCCTT ATCAAGTGGG ACATACGTTT GAACCAACGG AGCTGTCGTT ACATCAAGGA 
TCGACGACGG CACCGAACAT GCTAACCGAA GCGGATTTGA TTGCACTAAT GGAGAAGCAT 
GGTATCGGAA CCGACGCAAC TCATGCCGAA CACATCAACA CGATCAAGGA ACGTGGATAC 
ATCGGGGAAG CTGAAGGCAG ATATCTAGTT CCGGGAACAC TAGGTATGGG ATTGGTAGAA 
GGCTACGAAG CGATGGAGCT TACGTTAGCA CAGCCAGAAC TTCGAGCCGG ACTCGAAGCG 
GATCTAAAAT TGATATGCGA AGGAAGAAAG GATCCCAAGG TTGTACTAGC AGAGCAAATT 
CAGAAATACA AAGAAGCCTA CCGAGTGATA ACTCAAAAGG CGAGAGCCCT CGATCAGGCT 
ATGTCACAAC GGTTGAATGA AGCGCCCAAA GATGCTCCTA CGAATCAGTT TGCTTCCACT 
GGAAACAATC CTCTACAGGA AGTTTGCAAG TGTCCCAAAT GCTCACAGAT GATGGCTCTC 
AAGAAGAAGA AAGATGGGAA TAGCTATTAC TTGGGTTGTC TGGGATTCCC CGACTGTAGA 
AACACCGTTT GGTTTGATGA GAACATCAAG GAAGCTTCCG GAACTGACGA AATATGTCCA 
AGATGTGGAG GATCTAACAA GAAAATGACC ATCAAGTTCA AACAGATTCG ATTTTATGGA 
TTGTTGAACC ACATGAGCGC CGATGCGTAC AAAACGTGCA TCGTCTGTGA CAATAAGTTC 
CGCGAGGTGC TCGGAATCGA AGATAGCCTA GTGAAACAAT TCCGGTCAAG TGGCATACGA 
GCGGATGTGA CGATTCCAGC AAGGGGGAAT GCTCCCACGG GAAATACCAC CCGAGGCCCG 
GCCAATCCCC CAAATCGCCC ACCCCCGACT TTTCCCAGCA ATCCATCCAG CGGTCGAGGT 
ACCGGAAGCG GAACCAGCAA TAACGGTCGC AACGGTTGGG GCAACAACGA CGATGATGAC 
GATTTCCGTG GGGGAGGTGG AGGAGCTATC TCTACCGGAA GTCGTACCAA CAATACCTTG 
ACCAGCGGAT GGGGTACTGG TAATAGCAGT TGGAACTCTT CAAACACATC GCAAACCCGA 
CCAACCCCTG CAAGACCCAA TCAGCCGAAT TCCGACTCGA CCTGGAATAG AAACAGAAAC 
CCTCCCGCCG GAGCGGACGA TGGAGAGGTG CGATGTGGAT GCAATCTGCC GGCGAAATCC 
CTCACCGTCA AGAAGGACGG TCCCAATAAA GGGCGGCCAT TCTTCGCATG CCCAATGGAA 
CAAAGCAAGC AGTGCGGATT CTTCAAGTGG GGCGACGAGG ACAACACGCA ACAGGGAGGA 
TCCTCGGGTA GTTCCACTTG GGGTCAAAGC AGACCTCCAC CTCCTCCGCA GAATAATCAG 
CGCGGGGGTG GCGGAAGCTG GGGTGGATCC TCTTCGGACT GGGGACGAAC GAATAACAGT 
GGGAAATCGA AAGGAACCGG CAACAAAGGG GGAGACCGTC CGAAAGCAAC GAGAAAGTGC 
GGGTTCTGTA GGCAGGAGGG GCACACCAAG GCCAAGTGTC CGCAGCGGAG TAACATGGAT 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001658595.1
CDS	128..3193
Translation	MLRSLFNVRIRRFCSDTKPGNAAVMKYLNVAEKNDAAKNIAGLLSRGSSQRREGYSPYNKIYEFSYNIRGQPVQMVMTSVSGHLLNYEFLPSFRGWHSCNPEQLFDAPVRKSCTENMGKIKQTLEREVRGCQTLVIWTDCDREGENIGFEIIEVCRAIKPGIRVLRAKFSEITAASVKRAVENLAAPDELQSEAVNVRSELDLRIGAAFTRFQTLRLQNVFPAKIVNNLVSYGSCQIPTLGFVAHRYKEIEKFIPQEFWKIKLNHTIDELTVEFNWARNRLFDKQCCEAYLMLCQSEPLVKIVSVTNKPKNKWRPTPLDTVELEKLGSRKLKINAKQTMTIAEKLYTQGIISYPRTETNMFTNDMKLAPLVEMQTDNPEWGGFAQKVLQWGPNPRNGKKSDQAHPPIHPTKYVSNLTGDEKRIYELVVRHFLACISKDAVGSETVVNAVLADEEFTASGLCILERNYLEVYPYDRWNSREIHPYQVGHTFEPTELSLHQGSTTAPNMLTEADLIALMEKHGIGTDATHAEHINTIKERGYIGEAEGRYLVPGTLGMGLVEGYEAMELTLAQPELRAGLEADLKLICEGRKDPKVVLAEQIQKYKEAYRVITQKARALDQAMSQRLNEAPKDAPTNQFASTGNNPLQEVCKCPKCSQMMALKKKKDGNSYYLGCLGFPDCRNTVWFDENIKEASGTDEICPRCGGSNKKMTIKFKQIRFYGLLNHMSADAYKTCIVCDNKFREVLGIEDSLVKQFRSSGIRADVTIPARGNAPTGNTTRGPANPPNRPPPTFPSNPSSGRGTGSGTSNNGRNGWGNNDDDDDFRGGGGGAISTGSRTNNTLTSGWGTGNSSWNSSNTSQTRPTPARPNQPNSDSTWNRNRNPPAGADDGEVRCGCNLPAKSLTVKKDGPNKGRPFFACPMEQSKQCGFFKWGDEDNTQQGGSSGSSTWGQSRPPPPPQNNQRGGGGSWGGSSSDWGRTNNSGKSKGTGNKGGDRPKATRKCGFCRQEGHTKAKCPQRSNMDF

Target ORF information:

RefSeq Version	XM_001658545.2
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti DNA topoisomerase 3-alpha (LOC5569510), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001658545.2

ORF Insert Sequence:

ATGTTACGTT CGTTATTCAA CGTTAGAATA AGAAGATTTT GCTCAGATAC TAAACCGGGC 
AATGCCGCAG TCATGAAGTA CCTCAACGTA GCAGAGAAAA ACGATGCGGC CAAAAACATT 
GCCGGTTTAC TTTCGCGAGG GTCTTCCCAA CGGCGCGAAG GCTACTCCCC GTACAACAAG 
ATCTACGAGT TCAGTTACAA TATCCGGGGC CAGCCGGTCC AGATGGTAAT GACCTCCGTT 
TCGGGCCATT TGCTGAACTA TGAGTTTCTT CCTTCGTTCC GAGGATGGCA CAGTTGTAAT 
CCGGAGCAGT TGTTCGATGC TCCGGTTCGG AAGTCGTGCA CGGAAAACAT GGGCAAAATC 
AAACAGACTT TGGAAAGGGA AGTCCGAGGC TGCCAAACCC TGGTCATTTG GACGGATTGC 
GATCGCGAAG GAGAAAACAT TGGATTCGAG ATAATCGAGG TTTGTCGGGC CATAAAACCT 
GGGATCAGAG TTTTGAGGGC GAAGTTTTCG GAAATAACGG CGGCCTCGGT AAAGCGGGCG 
GTCGAAAATC TGGCCGCACC GGATGAACTG CAGAGTGAGG CCGTAAATGT GCGCAGCGAG 
TTGGATCTTC GGATCGGGGC TGCCTTTACC CGATTCCAGA CACTTCGGCT GCAGAATGTG 
TTTCCGGCGA AGATCGTCAA TAACCTGGTC TCGTACGGAA GTTGCCAGAT TCCGACGCTG 
GGCTTTGTCG CTCACCGATA TAAGGAGATT GAAAAGTTTA TTCCGCAGGA GTTTTGGAAA 
ATCAAGTTAA ATCACACCAT CGATGAGCTG ACGGTGGAGT TCAATTGGGC GAGGAATCGT 
CTTTTCGATA AGCAATGCTG TGAGGCGTAT TTGATGCTTT GTCAATCGGA ACCACTGGTT 
AAAATTGTTA GCGTTACGAA CAAACCGAAA AATAAGTGGC GTCCAACGCC ATTGGATACG 
GTCGAATTGG AAAAACTTGG ATCGAGAAAG CTGAAAATCA ATGCCAAGCA GACAATGACG 
ATAGCGGAAA AACTGTACAC CCAAGGTATC ATCAGTTATC CTCGTACCGA AACGAACATG 
TTTACGAACG ATATGAAACT AGCGCCACTG GTGGAGATGC AAACTGATAA TCCCGAGTGG 
GGTGGATTTG CACAGAAAGT GCTTCAATGG GGACCAAATC CTCGAAATGG CAAAAAATCC 
GATCAAGCGC ATCCTCCCAT TCATCCAACT AAGTATGTCT CCAACCTAAC TGGTGATGAG 
AAGCGGATAT ACGAACTAGT GGTTCGCCAC TTCCTGGCCT GCATAAGTAA GGATGCTGTC 
GGATCGGAAA CTGTCGTCAA TGCTGTTCTG GCTGATGAGG AATTCACGGC TTCCGGATTG 
TGCATTCTTG AGAGGAACTA TCTAGAAGTC TATCCGTATG ATCGATGGAA TTCAAGAGAG 
ATCCACCCTT ATCAAGTGGG ACATACGTTT GAACCAACGG AGCTGTCGTT ACATCAAGGA 
TCGACGACGG CACCGAACAT GCTAACCGAA GCGGATTTGA TTGCACTAAT GGAGAAGCAT 
GGTATCGGAA CCGACGCAAC TCATGCCGAA CACATCAACA CGATCAAGGA ACGTGGATAC 
ATCGGGGAAG CTGAAGGCAG ATATCTAGTT CCGGGAACAC TAGGTATGGG ATTGGTAGAA 
GGCTACGAAG CGATGGAGCT TACGTTAGCA CAGCCAGAAC TTCGAGCCGG ACTCGAAGCG 
GATCTAAAAT TGATATGCGA AGGAAGAAAG GATCCCAAGG TTGTACTAGC AGAGCAAATT 
CAGAAATACA AAGAAGCCTA CCGAGTGATA ACTCAAAAGG CGAGAGCCCT CGATCAGGCT 
ATGTCACAAC GGTTGAATGA AGCGCCCAAA GATGCTCCTA CGAATCAGTT TGCTTCCACT 
GGAAACAATC CTCTACAGGA AGTTTGCAAG TGTCCCAAAT GCTCACAGAT GATGGCTCTC 
AAGAAGAAGA AAGATGGGAA TAGCTATTAC TTGGGTTGTC TGGGATTCCC CGACTGTAGA 
AACACCGTTT GGTTTGATGA GAACATCAAG GAAGCTTCCG GAACTGACGA AATATGTCCA 
AGATGTGGAG GATCTAACAA GAAAATGACC ATCAAGTTCA AACAGATTCG ATTTTATGGA 
TTGTTGAACC ACATGAGCGC CGATGCGTAC AAAACGTGCA TCGTCTGTGA CAATAAGTTC 
CGCGAGGTGC TCGGAATCGA AGATAGCCTA GTGAAACAAT TCCGGTCAAG TGGCATACGA 
GCGGATGTGA CGATTCCAGC AAGGGGGAAT GCTCCCACGG GAAATACCAC CCGAGGCCCG 
GCCAATCCCC CAAATCGCCC ACCCCCGACT TTTCCCAGCA ATCCATCCAG CGGTCGAGGT 
ACCGGAAGCG GAACCAGCAA TAACGGTCGC AACGGTTGGG GCAACAACGA CGATGATGAC 
GATTTCCGTG GGGGAGGTGG AGGAGCTATC TCTACCGGAA GTCGTACCAA CAATACCTTG 
ACCAGCGGAT GGGGTACTGG TAATAGCAGT TGGAACTCTT CAAACACATC GCAAACCCGA 
CCAACCCCTG CAAGACCCAA TCAGCCGAAT TCCGACTCGA CCTGGAATAG AAACAGAAAC 
CCTCCCGCCG GAGCGGACGA TGGAGAGGTG CGATGTGGAT GCAATCTGCC GGCGAAATCC 
CTCACCGTCA AGAAGGACGG TCCCAATAAA GGGCGGCCAT TCTTCGCATG CCCAATGGAA 
CAAAGCAAGC AGTGCGGATT CTTCAAGTGG GGCGACGAGG ACAACACGCA ACAGGGAGGA 
TCCTCGGGTA GTTCCACTTG GGGTCAAAGC AGACCTCCAC CTCCTCCGCA GAATAATCAG 
CGCGGGGGTG GCGGAAGCTG GGGTGGATCC TCTTCGGACT GGGGACGAAC GAATAACAGT 
GGGAAATCGA AAGGAACCGG CAACAAAGGG GGAGACCGTC CGAAAGCAAC GAGAAAGTGC 
GGGTTCTGTA GGCAGGAGGG GCACACCAAG GCCAAGTGTC CGCAGCGGAG TAACATGGAT 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAEL_AAEL007683 ( XM_001658545.1 ) cDNA ORF clone, Aedes aegypti(Yellow fever mosquito) -> XP_001658595.1 Aedes aegypti AAEL007683-RA partial mRNA.

Price & Availability↑

CloneID	OAe05200
Clone ID Related Accession (Same CDS sequence)	XM_001658545.2 , XM_001658545.1
Accession Version	XM_001658545.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-04
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL007683-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001810989). COMPLETENESS: incomplete on the 3' end.

ATGTTACGTT CGTTATTCAA CGTTAGAATA AGAAGATTTT GCTCAGATAC TAAACCGGGC 
AATGCCGCAG TCATGAAGTA CCTCAACGTA GCAGAGAAAA ACGATGCGGC CAAAAACATT 
GCCGGTTTAC TTTCGCGAGG GTCTTCCCAA CGGCGCGAAG GCTACTCCCC GTACAACAAG 
ATCTACGAGT TCAGTTACAA TATCCGGGGC CAGCCGGTCC AGATGGTAAT GACCTCCGTT 
TCGGGCCATT TGCTGAACTA TGAGTTTCTT CCTTCGTTCC GAGGATGGCA CAGTTGTAAT 
CCGGAGCAGT TGTTCGATGC TCCGGTTCGG AAGTCGTGCA CGGAAAACAT GGGCAAAATC 
AAACAGACTT TGGAAAGGGA AGTCCGAGGC TGCCAAACCC TGGTCATTTG GACGGATTGC 
GATCGCGAAG GAGAAAACAT TGGATTCGAG ATAATCGAGG TTTGTCGGGC CATAAAACCT 
GGGATCAGAG TTTTGAGGGC GAAGTTTTCG GAAATAACGG CGGCCTCGGT AAAGCGGGCG 
GTCGAAAATC TGGCCGCACC GGATGAACTG CAGAGTGAGG CCGTAAATGT GCGCAGCGAG 
TTGGATCTTC GGATCGGGGC TGCCTTTACC CGATTCCAGA CACTTCGGCT GCAGAATGTG 
TTTCCGGCGA AGATCGTCAA TAACCTGGTC TCGTACGGAA GTTGCCAGAT TCCGACGCTG 
GGCTTTGTCG CTCACCGATA TAAGGAGATT GAAAAGTTTA TTCCGCAGGA GTTTTGGAAA 
ATCAAGTTAA ATCACACCAT CGATGAGCTG ACGGTGGAGT TCAATTGGGC GAGGAATCGT 
CTTTTCGATA AGCAATGCTG TGAGGCGTAT TTGATGCTTT GTCAATCGGA ACCACTGGTT 
AAAATTGTTA GCGTTACGAA CAAACCGAAA AATAAGTGGC GTCCAACGCC ATTGGATACG 
GTCGAATTGG AAAAACTTGG ATCGAGAAAG CTGAAAATCA ATGCCAAGCA GACAATGACG 
ATAGCGGAAA AACTGTACAC CCAAGGTATC ATCAGTTATC CTCGTACCGA AACGAACATG 
TTTACGAACG ATATGAAACT AGCGCCACTG GTGGAGATGC AAACTGATAA TCCCGAGTGG 
GGTGGATTTG CACAGAAAGT GCTTCAATGG GGACCAAATC CTCGAAATGG CAAAAAATCC 
GATCAAGCGC ATCCTCCCAT TCATCCAACT AAGTATGTCT CCAACCTAAC TGGTGATGAG 
AAGCGGATAT ACGAACTAGT GGTTCGCCAC TTCCTGGCCT GCATAAGTAA GGATGCTGTC 
GGATCGGAAA CTGTCGTCAA TGCTGTTCTG GCTGATGAGG AATTCACGGC TTCCGGATTG 
TGCATTCTTG AGAGGAACTA TCTAGAAGTC TATCCGTATG ATCGATGGAA TTCAAGAGAG 
ATCCACCCTT ATCAAGTGGG ACATACGTTT GAACCAACGG AGCTGTCGTT ACATCAAGGA 
TCGACGACGG CACCGAACAT GCTAACCGAA GCGGATTTGA TTGCACTAAT GGAGAAGCAT 
GGTATCGGAA CCGACGCAAC TCATGCCGAA CACATCAACA CGATCAAGGA ACGTGGATAC 
ATCGGGGAAG CTGAAGGCAG ATATCTAGTT CCGGGAACAC TAGGTATGGG ATTGGTAGAA 
GGCTACGAAG CGATGGAGCT TACGTTAGCA CAGCCAGAAC TTCGAGCCGG ACTCGAAGCG 
GATCTAAAAT TGATATGCGA AGGAAGAAAG GATCCCAAGG TTGTACTAGC AGAGCAAATT 
CAGAAATACA AAGAAGCCTA CCGAGTGATA ACTCAAAAGG CGAGAGCCCT CGATCAGGCT 
ATGTCACAAC GGTTGAATGA AGCGCCCAAA GATGCTCCTA CGAATCAGTT TGCTTCCACT 
GGAAACAATC CTCTACAGGA AGTTTGCAAG TGTCCCAAAT GCTCACAGAT GATGGCTCTC 
AAGAAGAAGA AAGATGGGAA TAGCTATTAC TTGGGTTGTC TGGGATTCCC CGACTGTAGA 
AACACCGTTT GGTTTGATGA GAACATCAAG GAAGCTTCCG GAACTGACGA AATATGTCCA 
AGATGTGGAG GATCTAACAA GAAAATGACC ATCAAGTTCA AACAGATTCG ATTTTATGGA 
TTGTTGAACC ACATGAGCGC CGATGCGTAC AAAACGTGCA TCGTCTGTGA CAATAAGTTC 
CGCGAGGTGC TCGGAATCGA AGATAGCCTA GTGAAACAAT TCCGGTCAAG TGGCATACGA 
GCGGATGTGA CGATTCCAGC AAGGGGGAAT GCTCCCACGG GAAATACCAC CCGAGGCCCG 
GCCAATCCCC CAAATCGCCC ACCCCCGACT TTTCCCAGCA ATCCATCCAG CGGTCGAGGT 
ACCGGAAGCG GAACCAGCAA TAACGGTCGC AACGGTTGGG GCAACAACGA CGATGATGAC 
GATTTCCGTG GGGGAGGTGG AGGAGCTATC TCTACCGGAA GTCGTACCAA CAATACCTTG 
ACCAGCGGAT GGGGTACTGG TAATAGCAGT TGGAACTCTT CAAACACATC GCAAACCCGA 
CCAACCCCTG CAAGACCCAA TCAGCCGAAT TCCGACTCGA CCTGGAATAG AAACAGAAAC 
CCTCCCGCCG GAGCGGACGA TGGAGAGGTG CGATGTGGAT GCAATCTGCC GGCGAAATCC 
CTCACCGTCA AGAAGGACGG TCCCAATAAA GGGCGGCCAT TCTTCGCATG CCCAATGGAA 
CAAAGCAAGC AGTGCGGATT CTTCAAGTGG GGCGACGAGG ACAACACGCA ACAGGGAGGA 
TCCTCGGGTA GTTCCACTTG GGGTCAAAGC AGACCTCCAC CTCCTCCGCA GAATAATCAG 
CGCGGGGGTG GCGGAAGCTG GGGTGGATCC TCTTCGGACT GGGGACGAAC GAATAACAGT 
GGGAAATCGA AAGGAACCGG CAACAAAGGG GGAGACCGTC CGAAAGCAAC GAGAAAGTGC 
GGGTTCTGTA GGCAGGAGGG GCACACCAAG GCCAAGTGTC CGCAGCGGAG TAACATGGAT 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001658595.1
CDS	122..3187
Translation	MLRSLFNVRIRRFCSDTKPGNAAVMKYLNVAEKNDAAKNIAGLLSRGSSQRREGYSPYNKIYEFSYNIRGQPVQMVMTSVSGHLLNYEFLPSFRGWHSCNPEQLFDAPVRKSCTENMGKIKQTLEREVRGCQTLVIWTDCDREGENIGFEIIEVCRAIKPGIRVLRAKFSEITAASVKRAVENLAAPDELQSEAVNVRSELDLRIGAAFTRFQTLRLQNVFPAKIVNNLVSYGSCQIPTLGFVAHRYKEIEKFIPQEFWKIKLNHTIDELTVEFNWARNRLFDKQCCEAYLMLCQSEPLVKIVSVTNKPKNKWRPTPLDTVELEKLGSRKLKINAKQTMTIAEKLYTQGIISYPRTETNMFTNDMKLAPLVEMQTDNPEWGGFAQKVLQWGPNPRNGKKSDQAHPPIHPTKYVSNLTGDEKRIYELVVRHFLACISKDAVGSETVVNAVLADEEFTASGLCILERNYLEVYPYDRWNSREIHPYQVGHTFEPTELSLHQGSTTAPNMLTEADLIALMEKHGIGTDATHAEHINTIKERGYIGEAEGRYLVPGTLGMGLVEGYEAMELTLAQPELRAGLEADLKLICEGRKDPKVVLAEQIQKYKEAYRVITQKARALDQAMSQRLNEAPKDAPTNQFASTGNNPLQEVCKCPKCSQMMALKKKKDGNSYYLGCLGFPDCRNTVWFDENIKEASGTDEICPRCGGSNKKMTIKFKQIRFYGLLNHMSADAYKTCIVCDNKFREVLGIEDSLVKQFRSSGIRADVTIPARGNAPTGNTTRGPANPPNRPPPTFPSNPSSGRGTGSGTSNNGRNGWGNNDDDDDFRGGGGGAISTGSRTNNTLTSGWGTGNSSWNSSNTSQTRPTPARPNQPNSDSTWNRNRNPPAGADDGEVRCGCNLPAKSLTVKKDGPNKGRPFFACPMEQSKQCGFFKWGDEDNTQQGGSSGSSTWGQSRPPPPPQNNQRGGGGSWGGSSSDWGRTNNSGKSKGTGNKGGDRPKATRKCGFCRQEGHTKAKCPQRSNMDF

Target ORF information:

RefSeq Version	XM_001658545.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL007683-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001658545.1

ORF Insert Sequence:

ATGTTACGTT CGTTATTCAA CGTTAGAATA AGAAGATTTT GCTCAGATAC TAAACCGGGC 
AATGCCGCAG TCATGAAGTA CCTCAACGTA GCAGAGAAAA ACGATGCGGC CAAAAACATT 
GCCGGTTTAC TTTCGCGAGG GTCTTCCCAA CGGCGCGAAG GCTACTCCCC GTACAACAAG 
ATCTACGAGT TCAGTTACAA TATCCGGGGC CAGCCGGTCC AGATGGTAAT GACCTCCGTT 
TCGGGCCATT TGCTGAACTA TGAGTTTCTT CCTTCGTTCC GAGGATGGCA CAGTTGTAAT 
CCGGAGCAGT TGTTCGATGC TCCGGTTCGG AAGTCGTGCA CGGAAAACAT GGGCAAAATC 
AAACAGACTT TGGAAAGGGA AGTCCGAGGC TGCCAAACCC TGGTCATTTG GACGGATTGC 
GATCGCGAAG GAGAAAACAT TGGATTCGAG ATAATCGAGG TTTGTCGGGC CATAAAACCT 
GGGATCAGAG TTTTGAGGGC GAAGTTTTCG GAAATAACGG CGGCCTCGGT AAAGCGGGCG 
GTCGAAAATC TGGCCGCACC GGATGAACTG CAGAGTGAGG CCGTAAATGT GCGCAGCGAG 
TTGGATCTTC GGATCGGGGC TGCCTTTACC CGATTCCAGA CACTTCGGCT GCAGAATGTG 
TTTCCGGCGA AGATCGTCAA TAACCTGGTC TCGTACGGAA GTTGCCAGAT TCCGACGCTG 
GGCTTTGTCG CTCACCGATA TAAGGAGATT GAAAAGTTTA TTCCGCAGGA GTTTTGGAAA 
ATCAAGTTAA ATCACACCAT CGATGAGCTG ACGGTGGAGT TCAATTGGGC GAGGAATCGT 
CTTTTCGATA AGCAATGCTG TGAGGCGTAT TTGATGCTTT GTCAATCGGA ACCACTGGTT 
AAAATTGTTA GCGTTACGAA CAAACCGAAA AATAAGTGGC GTCCAACGCC ATTGGATACG 
GTCGAATTGG AAAAACTTGG ATCGAGAAAG CTGAAAATCA ATGCCAAGCA GACAATGACG 
ATAGCGGAAA AACTGTACAC CCAAGGTATC ATCAGTTATC CTCGTACCGA AACGAACATG 
TTTACGAACG ATATGAAACT AGCGCCACTG GTGGAGATGC AAACTGATAA TCCCGAGTGG 
GGTGGATTTG CACAGAAAGT GCTTCAATGG GGACCAAATC CTCGAAATGG CAAAAAATCC 
GATCAAGCGC ATCCTCCCAT TCATCCAACT AAGTATGTCT CCAACCTAAC TGGTGATGAG 
AAGCGGATAT ACGAACTAGT GGTTCGCCAC TTCCTGGCCT GCATAAGTAA GGATGCTGTC 
GGATCGGAAA CTGTCGTCAA TGCTGTTCTG GCTGATGAGG AATTCACGGC TTCCGGATTG 
TGCATTCTTG AGAGGAACTA TCTAGAAGTC TATCCGTATG ATCGATGGAA TTCAAGAGAG 
ATCCACCCTT ATCAAGTGGG ACATACGTTT GAACCAACGG AGCTGTCGTT ACATCAAGGA 
TCGACGACGG CACCGAACAT GCTAACCGAA GCGGATTTGA TTGCACTAAT GGAGAAGCAT 
GGTATCGGAA CCGACGCAAC TCATGCCGAA CACATCAACA CGATCAAGGA ACGTGGATAC 
ATCGGGGAAG CTGAAGGCAG ATATCTAGTT CCGGGAACAC TAGGTATGGG ATTGGTAGAA 
GGCTACGAAG CGATGGAGCT TACGTTAGCA CAGCCAGAAC TTCGAGCCGG ACTCGAAGCG 
GATCTAAAAT TGATATGCGA AGGAAGAAAG GATCCCAAGG TTGTACTAGC AGAGCAAATT 
CAGAAATACA AAGAAGCCTA CCGAGTGATA ACTCAAAAGG CGAGAGCCCT CGATCAGGCT 
ATGTCACAAC GGTTGAATGA AGCGCCCAAA GATGCTCCTA CGAATCAGTT TGCTTCCACT 
GGAAACAATC CTCTACAGGA AGTTTGCAAG TGTCCCAAAT GCTCACAGAT GATGGCTCTC 
AAGAAGAAGA AAGATGGGAA TAGCTATTAC TTGGGTTGTC TGGGATTCCC CGACTGTAGA 
AACACCGTTT GGTTTGATGA GAACATCAAG GAAGCTTCCG GAACTGACGA AATATGTCCA 
AGATGTGGAG GATCTAACAA GAAAATGACC ATCAAGTTCA AACAGATTCG ATTTTATGGA 
TTGTTGAACC ACATGAGCGC CGATGCGTAC AAAACGTGCA TCGTCTGTGA CAATAAGTTC 
CGCGAGGTGC TCGGAATCGA AGATAGCCTA GTGAAACAAT TCCGGTCAAG TGGCATACGA 
GCGGATGTGA CGATTCCAGC AAGGGGGAAT GCTCCCACGG GAAATACCAC CCGAGGCCCG 
GCCAATCCCC CAAATCGCCC ACCCCCGACT TTTCCCAGCA ATCCATCCAG CGGTCGAGGT 
ACCGGAAGCG GAACCAGCAA TAACGGTCGC AACGGTTGGG GCAACAACGA CGATGATGAC 
GATTTCCGTG GGGGAGGTGG AGGAGCTATC TCTACCGGAA GTCGTACCAA CAATACCTTG 
ACCAGCGGAT GGGGTACTGG TAATAGCAGT TGGAACTCTT CAAACACATC GCAAACCCGA 
CCAACCCCTG CAAGACCCAA TCAGCCGAAT TCCGACTCGA CCTGGAATAG AAACAGAAAC 
CCTCCCGCCG GAGCGGACGA TGGAGAGGTG CGATGTGGAT GCAATCTGCC GGCGAAATCC 
CTCACCGTCA AGAAGGACGG TCCCAATAAA GGGCGGCCAT TCTTCGCATG CCCAATGGAA 
CAAAGCAAGC AGTGCGGATT CTTCAAGTGG GGCGACGAGG ACAACACGCA ACAGGGAGGA 
TCCTCGGGTA GTTCCACTTG GGGTCAAAGC AGACCTCCAC CTCCTCCGCA GAATAATCAG 
CGCGGGGGTG GCGGAAGCTG GGGTGGATCC TCTTCGGACT GGGGACGAAC GAATAACAGT 
GGGAAATCGA AAGGAACCGG CAACAAAGGG GGAGACCGTC CGAAAGCAAC GAGAAAGTGC 
GGGTTCTGTA GGCAGGAGGG GCACACCAAG GCCAAGTGTC CGCAGCGGAG TAACATGGAT 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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AAEL_AAEL007683 cDNA ORF clone, Aedes aegypti(Yellow fever mosquito)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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