RASAL2 gene cDNA ORF clone, Acinonyx jubatus(cheetah)

mRNA	Protein	Name
XM_015083312.1	XP_014938798.1	LOW QUALITY PROTEIN: ras GTPase-activating protein nGAP
XM_027074297.1	XP_026930098.1	ras GTPase-activating protein nGAP isoform X3
XM_027074298.1	XP_026930099.1	ras GTPase-activating protein nGAP isoform X4
XM_027074296.1	XP_026930097.1	ras GTPase-activating protein nGAP isoform X1
XM_027074300.1	XP_026930101.1	ras GTPase-activating protein nGAP isoform X6
XM_027074301.1	XP_026930102.1	ras GTPase-activating protein nGAP isoform X7
XM_027074303.1	XP_026930104.1	ras GTPase-activating protein nGAP isoform X9
XM_027074304.1	XP_026930105.1	ras GTPase-activating protein nGAP isoform X10
XM_015083312.2	XP_014938798.2	ras GTPase-activating protein nGAP isoform X2
XM_027074299.1	XP_026930100.1	ras GTPase-activating protein nGAP isoform X5
XM_027074302.1	XP_026930103.1	ras GTPase-activating protein nGAP isoform X8
XM_027074305.1	XP_026930106.1	ras GTPase-activating protein nGAP isoform X11

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAc231106	XM_027074296.1 Latest version!	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OAc231107	XM_015083312.2 Latest version!	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OAc231108	XM_027074297.1 Latest version!	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OAc231109	XM_027074298.1 Latest version!	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OAc231110	XM_027074299.1 Latest version!	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OAc231111	XM_027074300.1 Latest version!	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAc231112	XM_027074301.1 Latest version!	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAc231113	XM_027074302.1 Latest version!	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAc231114	XM_027074303.1 Latest version!	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OAc231115	XM_027074304.1 Latest version!	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAc231116	XM_027074305.1 Latest version!	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAc54184	XM_015083312.1 Latest version!	Acinonyx jubatus RAS protein activator like 2 (RASAL2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

RASAL2 ( XM_027074296.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026930097.1 Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc231106
Clone ID Related Accession (Same CDS sequence)	XM_027074296.1
Accession Version	XM_027074296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	ras GTPase-activating protein nGAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834759.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAAACGT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AATTCAGAGA TAGGAACAAG CTTAAAGAAT 
TATCCTTGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAAC 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTTTAG GTGGAAAGGG 
GGCAAAAGGG AGAGATGGCA GACTAATAAA GGAGACTTGG ATGAACCACA ACAAAAGGTC 
GTGCCAAAGA TGGTTGGTAA TAATGCTTCT TCCTCTGCTA ATGAGGTCCT TGGAACATCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCCGAGCCAG AACCCCGCTG CAGTATCAGA TTTGGTAGAC CGTGGCCGAC TTCAGTGAGG 
TCAGAGGAGT CTGAGCAGCA GGGATATTTC ATCCGTGGCA TTTTTTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TTTAAGAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCCACGCA TCGTCTGTCT TGCGGCCAGT CACCCTACAC GGAAACGACA 
ACGTGGGAGC GGAGGTACTG CATCCTCACG GACAGCCAGC TGGTGCTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACTAAAC TGGAGGTGCC GGCAGAAAGG TCCCCCCGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGTAACA CAGATGACAG GGCTCGTGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCCTTGC TGAGCCCTTG CAGTGCAGTA 
GAGTGTCTGG ATCTTGGCAG AGGAGAGCCC GTGTCAGTGA AACCACTCCA TAGTAGCATC 
CTTGGACAAG ATTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCCGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGCG AACATTTTGA ATTCTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTACGT CGGCCTAGTC AACATTCCCA CTGCCAGTGT GACTGGTCGC 
CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACCCCTACGC CCAACAAAGG AAAGACAGGC 
GGACCTTCTA TTCGGATTAA ATCACGGTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA 
AGTACCGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
CTGTACGAGT CAGATGAGAA CTGTGAGGTG GAGCCCAGCA GGTGTTCCTC TAGTGAACTG 
ATAGAGCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCGTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CGTCATGGAA GCAGCAGTGC 
CTGAACCGTG GCAAGCAGGA CATCAGCGAG CGGCTCATCA GTGCCTCGTT ATTTCTGCGT 
TTTCTGTGCC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TGATGCAGGA GTATCCTGAT 
GACCGCACAT CTCGGACTCT GACTCTAATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAATTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CGGACACCAT CTCGAACACG 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG 
TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CTCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTGAC TAATCCTACA 
CCAATACAAC AGCAACTAAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTC GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACTGAT AGCTACTTCA GAGGGAAAAC ATTACTGCTG 
GTGCAGCAAG CGTCCTCCCA GAGCATGACT TACTCTGAAA AGGATGAGAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAGG ACACTCACGC TGCTCAAGTG 
GAGCATGCAC CTGTCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGCATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGCGCACCC 
CAAGTGAGAA GGCCCCTGCA CCCGGCCTTG AACCAGCCAG GCAGCCTCCA GCCCTTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATTCCGG CAATGCCAAA GGCCTCTGTG 
GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAGGAT 
TTCAAACTCA GCGGGCCCAG CAATAGCAGT ATGGAAGATT TCACCAAACG CAGCACCCAG 
AGCGAGGATT TCTCCAGGCG GCATACTGTG CCAGACAGAC ACATACCTCT TGCTCTGCCA 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCACCATC CCTGCCACAT AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCAGCCCT CATGGCCGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCTTCCT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCGATCCA GAGACAACAA 
ACACAGCAGG TTCAGTCACC TGTGGACTCT GCCACAATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTTC TGAACAATGG ACAATATGAA GAGGATGTGG AAGAAACTGA GCAAAATCAA 
GATGAATCCA AGCATGCTGA GAAGTACGAG CAGGAAATCA CGAAGCTGAA GGAGCGCCTG 
CGAGTGTCCA GCCGGCGGCT GGAGGAGTAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA GTACAAGGCG CGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT CATGGCTGTG 
GAAGAGGAAC TGAAGAAAGA CCACGCTGAG ATGCAAGCCG TTATTGATGC AAAGCAAAAA 
ATAATTGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGGATCGT 
GTCCCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCACTG ACCCAAGTGA AGGAGCGATA 
CAGTGTGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026930097.1
CDS	309..4577
Translation	MESWKMSIFKRQTSPCPPSYRRSLSEPAYANSEIGTSLKNYPWRGPVKSKRKKNEHAVSNLGKPLGAYRWQTHPSFRWKGGKRERWQTNKGDLDEPQQKVVPKMVGNNASSSANEVLGTSTEKLNLVNTRSLRVPFTRSISEPEPRCSIRFGRPWPTSVRSEESEQQGYFIRGIFSTLSNGFSRMLSLRGESTNEPVREDVKGPPTHRLSCGQSPYTETTTWERRYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRARGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVEPSRCSSSELIEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHAPVMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALMAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTDRVPGFGQHQTDERTDPSEGAIQCAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_027074296.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027074296.1

ORF Insert Sequence:

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAAACGT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AATTCAGAGA TAGGAACAAG CTTAAAGAAT 
TATCCTTGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAAC 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTTTAG GTGGAAAGGG 
GGCAAAAGGG AGAGATGGCA GACTAATAAA GGAGACTTGG ATGAACCACA ACAAAAGGTC 
GTGCCAAAGA TGGTTGGTAA TAATGCTTCT TCCTCTGCTA ATGAGGTCCT TGGAACATCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCCGAGCCAG AACCCCGCTG CAGTATCAGA TTTGGTAGAC CGTGGCCGAC TTCAGTGAGG 
TCAGAGGAGT CTGAGCAGCA GGGATATTTC ATCCGTGGCA TTTTTTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TTTAAGAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCCACGCA TCGTCTGTCT TGCGGCCAGT CACCCTACAC GGAAACGACA 
ACGTGGGAGC GGAGGTACTG CATCCTCACG GACAGCCAGC TGGTGCTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACTAAAC TGGAGGTGCC GGCAGAAAGG TCCCCCCGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGTAACA CAGATGACAG GGCTCGTGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCCTTGC TGAGCCCTTG CAGTGCAGTA 
GAGTGTCTGG ATCTTGGCAG AGGAGAGCCC GTGTCAGTGA AACCACTCCA TAGTAGCATC 
CTTGGACAAG ATTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCCGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGCG AACATTTTGA ATTCTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTACGT CGGCCTAGTC AACATTCCCA CTGCCAGTGT GACTGGTCGC 
CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACCCCTACGC CCAACAAAGG AAAGACAGGC 
GGACCTTCTA TTCGGATTAA ATCACGGTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA 
AGTACCGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
CTGTACGAGT CAGATGAGAA CTGTGAGGTG GAGCCCAGCA GGTGTTCCTC TAGTGAACTG 
ATAGAGCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCGTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CGTCATGGAA GCAGCAGTGC 
CTGAACCGTG GCAAGCAGGA CATCAGCGAG CGGCTCATCA GTGCCTCGTT ATTTCTGCGT 
TTTCTGTGCC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TGATGCAGGA GTATCCTGAT 
GACCGCACAT CTCGGACTCT GACTCTAATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAATTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CGGACACCAT CTCGAACACG 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG 
TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CTCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTGAC TAATCCTACA 
CCAATACAAC AGCAACTAAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTC GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACTGAT AGCTACTTCA GAGGGAAAAC ATTACTGCTG 
GTGCAGCAAG CGTCCTCCCA GAGCATGACT TACTCTGAAA AGGATGAGAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAGG ACACTCACGC TGCTCAAGTG 
GAGCATGCAC CTGTCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGCATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGCGCACCC 
CAAGTGAGAA GGCCCCTGCA CCCGGCCTTG AACCAGCCAG GCAGCCTCCA GCCCTTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATTCCGG CAATGCCAAA GGCCTCTGTG 
GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAGGAT 
TTCAAACTCA GCGGGCCCAG CAATAGCAGT ATGGAAGATT TCACCAAACG CAGCACCCAG 
AGCGAGGATT TCTCCAGGCG GCATACTGTG CCAGACAGAC ACATACCTCT TGCTCTGCCA 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCACCATC CCTGCCACAT AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCAGCCCT CATGGCCGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCTTCCT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCGATCCA GAGACAACAA 
ACACAGCAGG TTCAGTCACC TGTGGACTCT GCCACAATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTTC TGAACAATGG ACAATATGAA GAGGATGTGG AAGAAACTGA GCAAAATCAA 
GATGAATCCA AGCATGCTGA GAAGTACGAG CAGGAAATCA CGAAGCTGAA GGAGCGCCTG 
CGAGTGTCCA GCCGGCGGCT GGAGGAGTAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA GTACAAGGCG CGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT CATGGCTGTG 
GAAGAGGAAC TGAAGAAAGA CCACGCTGAG ATGCAAGCCG TTATTGATGC AAAGCAAAAA 
ATAATTGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGGATCGT 
GTCCCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCACTG ACCCAAGTGA AGGAGCGATA 
CAGTGTGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015083312.2 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_014938798.2 Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAc231107
Clone ID Related Accession (Same CDS sequence)	XM_015083312.2
Accession Version	XM_015083312.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	ras GTPase-activating protein nGAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834759.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 13, 2018 this sequence version replaced XM_015083312.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAAACGT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AATTCAGAGA TAGGAACAAG CTTAAAGAAT 
TATCCTTGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAAC 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTTTAG GTGGAAAGGG 
GGCAAAAGGG AGAGATGGCA GACTAATAAA GGAGACTTGG ATGAACCACA ACAAAAGGTC 
GTGCCAAAGA TGGTTGGTAA TAATGCTTCT TCCTCTGCTA ATGAGGTCCT TGGAACATCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCCGAGCCAG AACCCCGCTG CAGTATCAGA TTTGGTAGAC CGTGGCCGAC TTCAGTGAGG 
TCAGAGGAGT CTGAGCAGCA GGGATATTTC ATCCGTGGCA TTTTTTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TTTAAGAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCCACGCA TCGTCTGTCT TGCGGCCAGT CACCCTACAC GGAAACGACA 
ACGTGGGAGC GGAGGTACTG CATCCTCACG GACAGCCAGC TGGTGCTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACTAAAC TGGAGGTGCC GGCAGAAAGG TCCCCCCGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGTAACA CAGATGACAG GGCTCGTGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCCTTGC TGAGCCCTTG CAGTGCAGTA 
GAGTGTCTGG ATCTTGGCAG AGGAGAGCCC GTGTCAGTGA AACCACTCCA TAGTAGCATC 
CTTGGACAAG ATTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCCGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGCG AACATTTTGA ATTCTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTACGT CGGCCTAGTC AACATTCCCA CTGCCAGTGT GACTGGTCGC 
CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACCCCTACGC CCAACAAAGG AAAGACAGGC 
GGACCTTCTA TTCGGATTAA ATCACGGTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA 
AGTACCGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
CTGTACGAGT CAGATGAGAA CTGTGAGGTG GAGCCCAGCA GGTGTTCCTC TAGTGAACTG 
ATAGAGCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCGTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CGTCATGGAA GCAGCAGTGC 
CTGAACCGTG GCAAGCAGGA CATCAGCGAG CGGCTCATCA GTGCCTCGTT ATTTCTGCGT 
TTTCTGTGCC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TGATGCAGGA GTATCCTGAT 
GACCGCACAT CTCGGACTCT GACTCTAATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAATTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CGGACACCAT CTCGAACACG 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG 
TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CTCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTGAC TAATCCTACA 
CCAATACAAC AGCAACTAAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTC GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACTGAT AGCTACTTCA GAGGGAAAAC ATTACTGCTG 
GTGCAGCAAG CGTCCTCCCA GAGCATGACT TACTCTGAAA AGGATGAGAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAGG ACACTCACGC TGCTCAAGTG 
GAGCATGCAC CTGTCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGCATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGCGCACCC 
CAAGTGAGAA GGCCCCTGCA CCCGGCCTTG AACCAGCCAG GCAGCCTCCA GCCCTTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATTCCGG CAATGCCAAA GGCCTCTGTG 
GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAGGAT 
TTCAAACTCA GCGGGCCCAG CAATAGCAGT ATGGAAGATT TCACCAAACG CAGCACCCAG 
AGCGAGGATT TCTCCAGGCG GCATACTGTG CCAGACAGAC ACATACCTCT TGCTCTGCCA 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCACCATC CCTGCCACAT AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCAGCCCT CATGGCCGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCTTCCT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCGATCCA GAGACAACAA 
ACACAGCAGG TTCAGTCACC TGTGGACTCT GCCACAATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTTC TGAACAATGG ACAATATGAA GAGGATGTGG AAGAAACTGA GCAAAATCAA 
GATGAATCCA AGCATGCTGA GAAGTACGAG CAGGAAATCA CGAAGCTGAA GGAGCGCCTG 
CGAGTGTCCA GCCGGCGGCT GGAGGAGTAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA GTACAAGGCG CGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT CATGGCTGTG 
GAAGAGGAAC TGAAGAAAGA CCACGCTGAG ATGCAAGCCG TTATTGATGC AAAGCAAAAA 
ATAATTGATG CACAGGAAAA ACGGATCGTG TCCCTGGATT CGGCCAACAC CAGACTGATG 
AGCGCACTGA CCCAAGTGAA GGAGCGATAC AGTGTGCAGG TCCGCAATGG CATCTCCCCC 
ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAACAG CAGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014938798.2
CDS	307..4566
Translation	MESWKMSIFKRQTSPCPPSYRRSLSEPAYANSEIGTSLKNYPWRGPVKSKRKKNEHAVSNLGKPLGAYRWQTHPSFRWKGGKRERWQTNKGDLDEPQQKVVPKMVGNNASSSANEVLGTSTEKLNLVNTRSLRVPFTRSISEPEPRCSIRFGRPWPTSVRSEESEQQGYFIRGIFSTLSNGFSRMLSLRGESTNEPVREDVKGPPTHRLSCGQSPYTETTTWERRYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRARGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVEPSRCSSSELIEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHAPVMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALMAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSVQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_015083312.2
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015083312.2

ORF Insert Sequence:

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAAACGT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AATTCAGAGA TAGGAACAAG CTTAAAGAAT 
TATCCTTGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAAC 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTTTAG GTGGAAAGGG 
GGCAAAAGGG AGAGATGGCA GACTAATAAA GGAGACTTGG ATGAACCACA ACAAAAGGTC 
GTGCCAAAGA TGGTTGGTAA TAATGCTTCT TCCTCTGCTA ATGAGGTCCT TGGAACATCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCCGAGCCAG AACCCCGCTG CAGTATCAGA TTTGGTAGAC CGTGGCCGAC TTCAGTGAGG 
TCAGAGGAGT CTGAGCAGCA GGGATATTTC ATCCGTGGCA TTTTTTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TTTAAGAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCCACGCA TCGTCTGTCT TGCGGCCAGT CACCCTACAC GGAAACGACA 
ACGTGGGAGC GGAGGTACTG CATCCTCACG GACAGCCAGC TGGTGCTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACTAAAC TGGAGGTGCC GGCAGAAAGG TCCCCCCGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGTAACA CAGATGACAG GGCTCGTGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCCTTGC TGAGCCCTTG CAGTGCAGTA 
GAGTGTCTGG ATCTTGGCAG AGGAGAGCCC GTGTCAGTGA AACCACTCCA TAGTAGCATC 
CTTGGACAAG ATTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCCGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGCG AACATTTTGA ATTCTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTACGT CGGCCTAGTC AACATTCCCA CTGCCAGTGT GACTGGTCGC 
CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACCCCTACGC CCAACAAAGG AAAGACAGGC 
GGACCTTCTA TTCGGATTAA ATCACGGTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA 
AGTACCGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
CTGTACGAGT CAGATGAGAA CTGTGAGGTG GAGCCCAGCA GGTGTTCCTC TAGTGAACTG 
ATAGAGCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCGTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CGTCATGGAA GCAGCAGTGC 
CTGAACCGTG GCAAGCAGGA CATCAGCGAG CGGCTCATCA GTGCCTCGTT ATTTCTGCGT 
TTTCTGTGCC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TGATGCAGGA GTATCCTGAT 
GACCGCACAT CTCGGACTCT GACTCTAATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAATTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CGGACACCAT CTCGAACACG 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG 
TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CTCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTGAC TAATCCTACA 
CCAATACAAC AGCAACTAAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTC GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACTGAT AGCTACTTCA GAGGGAAAAC ATTACTGCTG 
GTGCAGCAAG CGTCCTCCCA GAGCATGACT TACTCTGAAA AGGATGAGAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAGG ACACTCACGC TGCTCAAGTG 
GAGCATGCAC CTGTCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGCATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGCGCACCC 
CAAGTGAGAA GGCCCCTGCA CCCGGCCTTG AACCAGCCAG GCAGCCTCCA GCCCTTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATTCCGG CAATGCCAAA GGCCTCTGTG 
GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAGGAT 
TTCAAACTCA GCGGGCCCAG CAATAGCAGT ATGGAAGATT TCACCAAACG CAGCACCCAG 
AGCGAGGATT TCTCCAGGCG GCATACTGTG CCAGACAGAC ACATACCTCT TGCTCTGCCA 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCACCATC CCTGCCACAT AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCAGCCCT CATGGCCGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCTTCCT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCGATCCA GAGACAACAA 
ACACAGCAGG TTCAGTCACC TGTGGACTCT GCCACAATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTTC TGAACAATGG ACAATATGAA GAGGATGTGG AAGAAACTGA GCAAAATCAA 
GATGAATCCA AGCATGCTGA GAAGTACGAG CAGGAAATCA CGAAGCTGAA GGAGCGCCTG 
CGAGTGTCCA GCCGGCGGCT GGAGGAGTAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA GTACAAGGCG CGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT CATGGCTGTG 
GAAGAGGAAC TGAAGAAAGA CCACGCTGAG ATGCAAGCCG TTATTGATGC AAAGCAAAAA 
ATAATTGATG CACAGGAAAA ACGGATCGTG TCCCTGGATT CGGCCAACAC CAGACTGATG 
AGCGCACTGA CCCAAGTGAA GGAGCGATAC AGTGTGCAGG TCCGCAATGG CATCTCCCCC 
ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAACAG CAGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_027074297.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026930098.1 Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

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CloneID	OAc231108
Clone ID Related Accession (Same CDS sequence)	XM_027074297.1
Accession Version	XM_027074297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	ras GTPase-activating protein nGAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834759.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAAACGT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AATTCAGAGA TAGGAACAAG CTTAAAGAAT 
TATCCTTGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAAC 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTTTAG GTGGAAAGGG 
GGCAAAAGGG AGAGATGGCA GACTAATAAA GGAGACTTGG ATGAACCACA ACAAAAGGTC 
GTGCCAAAGA TGGTTGGTAA TAATGCTTCT TCCTCTGCTA ATGAGGTCCT TGGAACATCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCCGAGCCAG AACCCCGCTG CAGTATCAGA TTTGGTAGAC CGTGGCCGAC TTCAGTGAGG 
TCAGAGGAGT CTGAGCAGCA GGGATATTTC ATCCGTGGCA TTTTTTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TTTAAGAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCCACGCA TCGTCTGTCT TGCGGCCAGT CACCCTACAC GGAAACGACA 
ACGTGGGAGC GGAGGTACTG CATCCTCACG GACAGCCAGC TGGTGCTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACTAAAC TGGAGGTGCC GGCAGAAAGG TCCCCCCGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGTAACA CAGATGACAG GGCTCGTGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCCTTGC TGAGCCCTTG CAGTGCAGTA 
GAGTGTCTGG ATCTTGGCAG AGGAGAGCCC GTGTCAGTGA AACCACTCCA TAGTAGCATC 
CTTGGACAAG ATTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCCGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGCG AACATTTTGA ATTCTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTACGT CGGCCTAGTC AACATTCCCA CTGCCAGTGT GACTGGTCGC 
CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACCCCTACGC CCAACAAAGG AAAGACAGGC 
GGACCTTCTA TTCGGATTAA ATCACGGTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA 
AGTACCGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
CTGTACGAGT CAGATGAGAA CTGTGAGGTG GAGCCCAGCA GGTGTTCCTC TAGTGAACTG 
ATAGAGCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCGTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CGTCATGGAA GCAGCAGTGC 
CTGAACCGTG GCAAGCAGGA CATCAGCGAG CGGCTCATCA GTGCCTCGTT ATTTCTGCGT 
TTTCTGTGCC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TGATGCAGGA GTATCCTGAT 
GACCGCACAT CTCGGACTCT GACTCTAATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAATTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CGGACACCAT CTCGAACACG 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG 
TGGGAAGTAG TTTCCCAACT TGATAAGGCG ACCGTGGCAA AATTGGGACC TCTCCCTCGA 
GTTCTTGCTG ATATTACCAA GTCTCTGACT AATCCTACAC CAATACAACA GCAACTAAGA 
CGCTTCACTG AGCATAACTC CAGTCCAAAT GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG 
AAAATATTCG AAGACCCCAC TGACAGTGAT TTGCATAAAC TAAAATCTCC AAGCCAGGAC 
AACACTGATA GCTACTTCAG AGGGAAAACA TTACTGCTGG TGCAGCAAGC GTCCTCCCAG 
AGCATGACTT ACTCTGAAAA GGATGAGAAG GAAAGTAGCC TTCCTAACGG TCGGAGCATC 
TCCCTCATGG ACCTTCAGGA CACTCACGCT GCTCAAGTGG AGCATGCACC TGTCATGCTC 
GATGTGCCTA TGCGCTTAAC TGGAAGCCAG CTTTCCATAA CCCAGGTGGC CAGCATCAAA 
CAACTGCGGG AAACCCAGAG CACTCCCCAG AGCGCACCCC AAGTGAGAAG GCCCCTGCAC 
CCGGCCTTGA ACCAGCCAGG CAGCCTCCAG CCCTTGTCAT TCCAGAACCC TGTCTATCAC 
CTCAATAACC CAATTCCGGC AATGCCAAAG GCCTCTGTGG ATTCCAGTTT GGAGAACCTA 
AGCACTGCCA GTTCCAGAAG CCAAAGTAAC AGTGAGGATT TCAAACTCAG CGGGCCCAGC 
AATAGCAGTA TGGAAGATTT CACCAAACGC AGCACCCAGA GCGAGGATTT CTCCAGGCGG 
CATACTGTGC CAGACAGACA CATACCTCTT GCTCTGCCAC GGCAGAATAG TACCGGGCAG 
GCCCAGATCC GGAAAATGGA CCAGGCTGGG TTAGGTGCCC GAGCCAAAGC CCCACCATCC 
CTGCCACATA GTGCTTCCTT ACGTAGCACG GGGAGCATGT CGGTGGCCTC TGCAGCCCTC 
ATGGCCGAAC CTGTGCAGAA TGGGAGCCGG TCCCGGCAGC AGTCTTCCTC CTCCAGAGAG 
AGCCCTGTTC CCAAAGTGAG AGCGATCCAG AGACAACAAA CACAGCAGGT TCAGTCACCT 
GTGGACTCTG CCACAATGTC CCCAGTAGAG AGGACAGCAG CCTGGGTTCT GAACAATGGA 
CAATATGAAG AGGATGTGGA AGAAACTGAG CAAAATCAAG ATGAATCCAA GCATGCTGAG 
AAGTACGAGC AGGAAATCAC GAAGCTGAAG GAGCGCCTGC GAGTGTCCAG CCGGCGGCTG 
GAGGAGTATG AGCGCCGCTT GCTGGTGCAG GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG 
TACAAGGCGC GGCTGGAGGA CAGCGAGGAG CGCCTGCGCA GGCAGCAGGA GGAGAAGGAC 
AGCCAGATGA AAAGCATCAT CAGCAGGCTC ATGGCTGTGG AAGAGGAACT GAAGAAAGAC 
CACGCTGAGA TGCAAGCCGT TATTGATGCA AAGCAAAAAA TAATTGATGC ACAGGTCATA 
AATGGAGATG AGATTATTCA AACAGGAAAA ACGGATCGTG TCCCTGGATT CGGCCAACAC 
CAGACTGATG AGCGCACTGA CCCAAGTGAA GGAGCGATAC AGTGTGCAGG TCCGCAATGG 
CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026930098.1
CDS	308..4555
Translation	MESWKMSIFKRQTSPCPPSYRRSLSEPAYANSEIGTSLKNYPWRGPVKSKRKKNEHAVSNLGKPLGAYRWQTHPSFRWKGGKRERWQTNKGDLDEPQQKVVPKMVGNNASSSANEVLGTSTEKLNLVNTRSLRVPFTRSISEPEPRCSIRFGRPWPTSVRSEESEQQGYFIRGIFSTLSNGFSRMLSLRGESTNEPVREDVKGPPTHRLSCGQSPYTETTTWERRYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRARGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVEPSRCSSSELIEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHAPVMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALMAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTDRVPGFGQHQTDERTDPSEGAIQCAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_027074297.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027074297.1

ORF Insert Sequence:

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAAACGT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AATTCAGAGA TAGGAACAAG CTTAAAGAAT 
TATCCTTGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAAC 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTTTAG GTGGAAAGGG 
GGCAAAAGGG AGAGATGGCA GACTAATAAA GGAGACTTGG ATGAACCACA ACAAAAGGTC 
GTGCCAAAGA TGGTTGGTAA TAATGCTTCT TCCTCTGCTA ATGAGGTCCT TGGAACATCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCCGAGCCAG AACCCCGCTG CAGTATCAGA TTTGGTAGAC CGTGGCCGAC TTCAGTGAGG 
TCAGAGGAGT CTGAGCAGCA GGGATATTTC ATCCGTGGCA TTTTTTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TTTAAGAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCCACGCA TCGTCTGTCT TGCGGCCAGT CACCCTACAC GGAAACGACA 
ACGTGGGAGC GGAGGTACTG CATCCTCACG GACAGCCAGC TGGTGCTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACTAAAC TGGAGGTGCC GGCAGAAAGG TCCCCCCGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGTAACA CAGATGACAG GGCTCGTGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCCTTGC TGAGCCCTTG CAGTGCAGTA 
GAGTGTCTGG ATCTTGGCAG AGGAGAGCCC GTGTCAGTGA AACCACTCCA TAGTAGCATC 
CTTGGACAAG ATTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCCGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGCG AACATTTTGA ATTCTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTACGT CGGCCTAGTC AACATTCCCA CTGCCAGTGT GACTGGTCGC 
CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACCCCTACGC CCAACAAAGG AAAGACAGGC 
GGACCTTCTA TTCGGATTAA ATCACGGTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA 
AGTACCGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
CTGTACGAGT CAGATGAGAA CTGTGAGGTG GAGCCCAGCA GGTGTTCCTC TAGTGAACTG 
ATAGAGCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCGTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CGTCATGGAA GCAGCAGTGC 
CTGAACCGTG GCAAGCAGGA CATCAGCGAG CGGCTCATCA GTGCCTCGTT ATTTCTGCGT 
TTTCTGTGCC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TGATGCAGGA GTATCCTGAT 
GACCGCACAT CTCGGACTCT GACTCTAATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAATTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CGGACACCAT CTCGAACACG 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG 
TGGGAAGTAG TTTCCCAACT TGATAAGGCG ACCGTGGCAA AATTGGGACC TCTCCCTCGA 
GTTCTTGCTG ATATTACCAA GTCTCTGACT AATCCTACAC CAATACAACA GCAACTAAGA 
CGCTTCACTG AGCATAACTC CAGTCCAAAT GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG 
AAAATATTCG AAGACCCCAC TGACAGTGAT TTGCATAAAC TAAAATCTCC AAGCCAGGAC 
AACACTGATA GCTACTTCAG AGGGAAAACA TTACTGCTGG TGCAGCAAGC GTCCTCCCAG 
AGCATGACTT ACTCTGAAAA GGATGAGAAG GAAAGTAGCC TTCCTAACGG TCGGAGCATC 
TCCCTCATGG ACCTTCAGGA CACTCACGCT GCTCAAGTGG AGCATGCACC TGTCATGCTC 
GATGTGCCTA TGCGCTTAAC TGGAAGCCAG CTTTCCATAA CCCAGGTGGC CAGCATCAAA 
CAACTGCGGG AAACCCAGAG CACTCCCCAG AGCGCACCCC AAGTGAGAAG GCCCCTGCAC 
CCGGCCTTGA ACCAGCCAGG CAGCCTCCAG CCCTTGTCAT TCCAGAACCC TGTCTATCAC 
CTCAATAACC CAATTCCGGC AATGCCAAAG GCCTCTGTGG ATTCCAGTTT GGAGAACCTA 
AGCACTGCCA GTTCCAGAAG CCAAAGTAAC AGTGAGGATT TCAAACTCAG CGGGCCCAGC 
AATAGCAGTA TGGAAGATTT CACCAAACGC AGCACCCAGA GCGAGGATTT CTCCAGGCGG 
CATACTGTGC CAGACAGACA CATACCTCTT GCTCTGCCAC GGCAGAATAG TACCGGGCAG 
GCCCAGATCC GGAAAATGGA CCAGGCTGGG TTAGGTGCCC GAGCCAAAGC CCCACCATCC 
CTGCCACATA GTGCTTCCTT ACGTAGCACG GGGAGCATGT CGGTGGCCTC TGCAGCCCTC 
ATGGCCGAAC CTGTGCAGAA TGGGAGCCGG TCCCGGCAGC AGTCTTCCTC CTCCAGAGAG 
AGCCCTGTTC CCAAAGTGAG AGCGATCCAG AGACAACAAA CACAGCAGGT TCAGTCACCT 
GTGGACTCTG CCACAATGTC CCCAGTAGAG AGGACAGCAG CCTGGGTTCT GAACAATGGA 
CAATATGAAG AGGATGTGGA AGAAACTGAG CAAAATCAAG ATGAATCCAA GCATGCTGAG 
AAGTACGAGC AGGAAATCAC GAAGCTGAAG GAGCGCCTGC GAGTGTCCAG CCGGCGGCTG 
GAGGAGTATG AGCGCCGCTT GCTGGTGCAG GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG 
TACAAGGCGC GGCTGGAGGA CAGCGAGGAG CGCCTGCGCA GGCAGCAGGA GGAGAAGGAC 
AGCCAGATGA AAAGCATCAT CAGCAGGCTC ATGGCTGTGG AAGAGGAACT GAAGAAAGAC 
CACGCTGAGA TGCAAGCCGT TATTGATGCA AAGCAAAAAA TAATTGATGC ACAGGTCATA 
AATGGAGATG AGATTATTCA AACAGGAAAA ACGGATCGTG TCCCTGGATT CGGCCAACAC 
CAGACTGATG AGCGCACTGA CCCAAGTGAA GGAGCGATAC AGTGTGCAGG TCCGCAATGG 
CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_027074298.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026930099.1 Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

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CloneID	OAc231109
Clone ID Related Accession (Same CDS sequence)	XM_027074298.1
Accession Version	XM_027074298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	ras GTPase-activating protein nGAP isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834759.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAAACGT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AATTCAGAGA TAGGAACAAG CTTAAAGAAT 
TATCCTTGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAAC 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTTTAG GTGGAAAGGG 
GGCAAAAGGG AGAGATGGCA GACTAATAAA GGAGACTTGG ATGAACCACA ACAAAAGGTC 
GTGCCAAAGA TGGTTGGTAA TAATGCTTCT TCCTCTGCTA ATGAGGTCCT TGGAACATCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCCGAGCCAG AACCCCGCTG CAGTATCAGA TTTGGTAGAC CGTGGCCGAC TTCAGTGAGG 
TCAGAGGAGT CTGAGCAGCA GGGATATTTC ATCCGTGGCA TTTTTTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TTTAAGAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCCACGCA TCGTCTGTCT TGCGGCCAGT CACCCTACAC GGAAACGACA 
ACGTGGGAGC GGAGGTACTG CATCCTCACG GACAGCCAGC TGGTGCTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACTAAAC TGGAGGTGCC GGCAGAAAGG TCCCCCCGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGTAACA CAGATGACAG GGCTCGTGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCCTTGC TGAGCCCTTG CAGTGCAGTA 
GAGTGTCTGG ATCTTGGCAG AGGAGAGCCC GTGTCAGTGA AACCACTCCA TAGTAGCATC 
CTTGGACAAG ATTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCCGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGCG AACATTTTGA ATTCTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTACGT CGGCCTAGTC AACATTCCCA CTGCCAGTGT GACTGGTCGC 
CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACCCCTACGC CCAACAAAGG AAAGACAGGC 
GGACCTTCTA TTCGGATTAA ATCACGGTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA 
AGTACCGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
CTGTACGAGT CAGATGAGAA CTGTGAGGTG GAGCCCAGCA GGTGTTCCTC TAGTGAACTG 
ATAGAGCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCGTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CGTCATGGAA GCAGCAGTGC 
CTGAACCGTG GCAAGCAGGA CATCAGCGAG CGGCTCATCA GTGCCTCGTT ATTTCTGCGT 
TTTCTGTGCC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TGATGCAGGA GTATCCTGAT 
GACCGCACAT CTCGGACTCT GACTCTAATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAATTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CGGACACCAT CTCGAACACG 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG 
TGGGAAGTAG TTTCCCAACT TGATAAGGCG ACCGTGGCAA AATTGGGACC TCTCCCTCGA 
GTTCTTGCTG ATATTACCAA GTCTCTGACT AATCCTACAC CAATACAACA GCAACTAAGA 
CGCTTCACTG AGCATAACTC CAGTCCAAAT GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG 
AAAATATTCG AAGACCCCAC TGACAGTGAT TTGCATAAAC TAAAATCTCC AAGCCAGGAC 
AACACTGATA GCTACTTCAG AGGGAAAACA TTACTGCTGG TGCAGCAAGC GTCCTCCCAG 
AGCATGACTT ACTCTGAAAA GGATGAGAAG GAAAGTAGCC TTCCTAACGG TCGGAGCATC 
TCCCTCATGG ACCTTCAGGA CACTCACGCT GCTCAAGTGG AGCATGCACC TGTCATGCTC 
GATGTGCCTA TGCGCTTAAC TGGAAGCCAG CTTTCCATAA CCCAGGTGGC CAGCATCAAA 
CAACTGCGGG AAACCCAGAG CACTCCCCAG AGCGCACCCC AAGTGAGAAG GCCCCTGCAC 
CCGGCCTTGA ACCAGCCAGG CAGCCTCCAG CCCTTGTCAT TCCAGAACCC TGTCTATCAC 
CTCAATAACC CAATTCCGGC AATGCCAAAG GCCTCTGTGG ATTCCAGTTT GGAGAACCTA 
AGCACTGCCA GTTCCAGAAG CCAAAGTAAC AGTGAGGATT TCAAACTCAG CGGGCCCAGC 
AATAGCAGTA TGGAAGATTT CACCAAACGC AGCACCCAGA GCGAGGATTT CTCCAGGCGG 
CATACTGTGC CAGACAGACA CATACCTCTT GCTCTGCCAC GGCAGAATAG TACCGGGCAG 
GCCCAGATCC GGAAAATGGA CCAGGCTGGG TTAGGTGCCC GAGCCAAAGC CCCACCATCC 
CTGCCACATA GTGCTTCCTT ACGTAGCACG GGGAGCATGT CGGTGGCCTC TGCAGCCCTC 
ATGGCCGAAC CTGTGCAGAA TGGGAGCCGG TCCCGGCAGC AGTCTTCCTC CTCCAGAGAG 
AGCCCTGTTC CCAAAGTGAG AGCGATCCAG AGACAACAAA CACAGCAGGT TCAGTCACCT 
GTGGACTCTG CCACAATGTC CCCAGTAGAG AGGACAGCAG CCTGGGTTCT GAACAATGGA 
CAATATGAAG AGGATGTGGA AGAAACTGAG CAAAATCAAG ATGAATCCAA GCATGCTGAG 
AAGTACGAGC AGGAAATCAC GAAGCTGAAG GAGCGCCTGC GAGTGTCCAG CCGGCGGCTG 
GAGGAGTATG AGCGCCGCTT GCTGGTGCAG GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG 
TACAAGGCGC GGCTGGAGGA CAGCGAGGAG CGCCTGCGCA GGCAGCAGGA GGAGAAGGAC 
AGCCAGATGA AAAGCATCAT CAGCAGGCTC ATGGCTGTGG AAGAGGAACT GAAGAAAGAC 
CACGCTGAGA TGCAAGCCGT TATTGATGCA AAGCAAAAAA TAATTGATGC ACAGGAAAAA 
CGGATCGTGT CCCTGGATTC GGCCAACACC AGACTGATGA GCGCACTGAC CCAAGTGAAG 
GAGCGATACA GTGTGCAGGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC 
ATCACGGAGA ATGGTGAATT CAAAAACAGC AGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026930099.1
CDS	307..4545
Translation	MESWKMSIFKRQTSPCPPSYRRSLSEPAYANSEIGTSLKNYPWRGPVKSKRKKNEHAVSNLGKPLGAYRWQTHPSFRWKGGKRERWQTNKGDLDEPQQKVVPKMVGNNASSSANEVLGTSTEKLNLVNTRSLRVPFTRSISEPEPRCSIRFGRPWPTSVRSEESEQQGYFIRGIFSTLSNGFSRMLSLRGESTNEPVREDVKGPPTHRLSCGQSPYTETTTWERRYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRARGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVEPSRCSSSELIEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHAPVMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALMAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSVQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_027074298.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027074298.1

ORF Insert Sequence:

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAAACGT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AATTCAGAGA TAGGAACAAG CTTAAAGAAT 
TATCCTTGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAAC 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTTTAG GTGGAAAGGG 
GGCAAAAGGG AGAGATGGCA GACTAATAAA GGAGACTTGG ATGAACCACA ACAAAAGGTC 
GTGCCAAAGA TGGTTGGTAA TAATGCTTCT TCCTCTGCTA ATGAGGTCCT TGGAACATCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCCGAGCCAG AACCCCGCTG CAGTATCAGA TTTGGTAGAC CGTGGCCGAC TTCAGTGAGG 
TCAGAGGAGT CTGAGCAGCA GGGATATTTC ATCCGTGGCA TTTTTTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TTTAAGAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCCACGCA TCGTCTGTCT TGCGGCCAGT CACCCTACAC GGAAACGACA 
ACGTGGGAGC GGAGGTACTG CATCCTCACG GACAGCCAGC TGGTGCTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACTAAAC TGGAGGTGCC GGCAGAAAGG TCCCCCCGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGTAACA CAGATGACAG GGCTCGTGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCCTTGC TGAGCCCTTG CAGTGCAGTA 
GAGTGTCTGG ATCTTGGCAG AGGAGAGCCC GTGTCAGTGA AACCACTCCA TAGTAGCATC 
CTTGGACAAG ATTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCCGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGCG AACATTTTGA ATTCTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTACGT CGGCCTAGTC AACATTCCCA CTGCCAGTGT GACTGGTCGC 
CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACCCCTACGC CCAACAAAGG AAAGACAGGC 
GGACCTTCTA TTCGGATTAA ATCACGGTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA 
AGTACCGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
CTGTACGAGT CAGATGAGAA CTGTGAGGTG GAGCCCAGCA GGTGTTCCTC TAGTGAACTG 
ATAGAGCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCGTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CGTCATGGAA GCAGCAGTGC 
CTGAACCGTG GCAAGCAGGA CATCAGCGAG CGGCTCATCA GTGCCTCGTT ATTTCTGCGT 
TTTCTGTGCC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TGATGCAGGA GTATCCTGAT 
GACCGCACAT CTCGGACTCT GACTCTAATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAATTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CGGACACCAT CTCGAACACG 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG 
TGGGAAGTAG TTTCCCAACT TGATAAGGCG ACCGTGGCAA AATTGGGACC TCTCCCTCGA 
GTTCTTGCTG ATATTACCAA GTCTCTGACT AATCCTACAC CAATACAACA GCAACTAAGA 
CGCTTCACTG AGCATAACTC CAGTCCAAAT GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG 
AAAATATTCG AAGACCCCAC TGACAGTGAT TTGCATAAAC TAAAATCTCC AAGCCAGGAC 
AACACTGATA GCTACTTCAG AGGGAAAACA TTACTGCTGG TGCAGCAAGC GTCCTCCCAG 
AGCATGACTT ACTCTGAAAA GGATGAGAAG GAAAGTAGCC TTCCTAACGG TCGGAGCATC 
TCCCTCATGG ACCTTCAGGA CACTCACGCT GCTCAAGTGG AGCATGCACC TGTCATGCTC 
GATGTGCCTA TGCGCTTAAC TGGAAGCCAG CTTTCCATAA CCCAGGTGGC CAGCATCAAA 
CAACTGCGGG AAACCCAGAG CACTCCCCAG AGCGCACCCC AAGTGAGAAG GCCCCTGCAC 
CCGGCCTTGA ACCAGCCAGG CAGCCTCCAG CCCTTGTCAT TCCAGAACCC TGTCTATCAC 
CTCAATAACC CAATTCCGGC AATGCCAAAG GCCTCTGTGG ATTCCAGTTT GGAGAACCTA 
AGCACTGCCA GTTCCAGAAG CCAAAGTAAC AGTGAGGATT TCAAACTCAG CGGGCCCAGC 
AATAGCAGTA TGGAAGATTT CACCAAACGC AGCACCCAGA GCGAGGATTT CTCCAGGCGG 
CATACTGTGC CAGACAGACA CATACCTCTT GCTCTGCCAC GGCAGAATAG TACCGGGCAG 
GCCCAGATCC GGAAAATGGA CCAGGCTGGG TTAGGTGCCC GAGCCAAAGC CCCACCATCC 
CTGCCACATA GTGCTTCCTT ACGTAGCACG GGGAGCATGT CGGTGGCCTC TGCAGCCCTC 
ATGGCCGAAC CTGTGCAGAA TGGGAGCCGG TCCCGGCAGC AGTCTTCCTC CTCCAGAGAG 
AGCCCTGTTC CCAAAGTGAG AGCGATCCAG AGACAACAAA CACAGCAGGT TCAGTCACCT 
GTGGACTCTG CCACAATGTC CCCAGTAGAG AGGACAGCAG CCTGGGTTCT GAACAATGGA 
CAATATGAAG AGGATGTGGA AGAAACTGAG CAAAATCAAG ATGAATCCAA GCATGCTGAG 
AAGTACGAGC AGGAAATCAC GAAGCTGAAG GAGCGCCTGC GAGTGTCCAG CCGGCGGCTG 
GAGGAGTATG AGCGCCGCTT GCTGGTGCAG GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG 
TACAAGGCGC GGCTGGAGGA CAGCGAGGAG CGCCTGCGCA GGCAGCAGGA GGAGAAGGAC 
AGCCAGATGA AAAGCATCAT CAGCAGGCTC ATGGCTGTGG AAGAGGAACT GAAGAAAGAC 
CACGCTGAGA TGCAAGCCGT TATTGATGCA AAGCAAAAAA TAATTGATGC ACAGGAAAAA 
CGGATCGTGT CCCTGGATTC GGCCAACACC AGACTGATGA GCGCACTGAC CCAAGTGAAG 
GAGCGATACA GTGTGCAGGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC 
ATCACGGAGA ATGGTGAATT CAAAAACAGC AGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_027074299.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026930100.1 Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OAc231110
Clone ID Related Accession (Same CDS sequence)	XM_027074299.1
Accession Version	XM_027074299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	ras GTPase-activating protein nGAP isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834759.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAAACGT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AATTCAGAGA TAGGAACAAG CTTAAAGAAT 
TATCCTTGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAAC 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTTTAG GTGGAAAGGG 
GGCAAAAGGG AGAGATGGCA GACTAATAAA GGAGACTTGG ATGAACCACA ACAAAAGGTC 
GTGCCAAAGA TGGTTGGTAA TAATGCTTCT TCCTCTGCTA ATGAGGTCCT TGGAACATCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCCGAGCCAG AACCCCGCTG CAGTATCAGA TTTGGTAGAC CGTGGCCGAC TTCAGTGAGG 
TCAGAGGAGT CTGAGCAGCA GGGATATTTC ATCCGTGGCA TTTTTTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TTTAAGAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCCACGCA TCGTCTGTCT TGCGGCCAGT CACCCTACAC GGAAACGACA 
ACGTGGGAGC GGAGGTACTG CATCCTCACG GACAGCCAGC TGGTGCTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACTAAAC TGGAGGTGCC GGCAGAAAGG TCCCCCCGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGTAACA CAGATGACAG GGCTCGTGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCCTTGC TGAGCCCTTG CAGTGCAGTA 
GAGTGTCTGG ATCTTGGCAG AGGAGAGCCC GTGTCAGTGA AACCACTCCA TAGTAGCATC 
CTTGGACAAG ATTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCCGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGCG AACATTTTGA ATTCTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTACGT CGGCCTAGTC AACATTCCCA CTGCCAGTGT GACTGGTCGC 
CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACCCCTACGC CCAACAAAGG AAAGACAGGC 
GGACCTTCTA TTCGGATTAA ATCACGGTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA 
AGTACCGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
CTGTACGAGT CAGATGAGAA CTGTGAGGTG GAGCCCAGCA GGTGTTCCTC TAGTGAACTG 
ATAGAGCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCGTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CGTCATGGAA GCAGCAGTGC 
CTGAACCGTG GCAAGCAGGA CATCAGCGAG CGGCTCATCA GTGCCTCGTT ATTTCTGCGT 
TTTCTGTGCC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TGATGCAGGA GTATCCTGAT 
GACCGCACAT CTCGGACTCT GACTCTAATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAATTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CGGACACCAT CTCGAACACG 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG 
TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CTCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTGAC TAATCCTACA 
CCAATACAAC AGCAACTAAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTC GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACTGAT AGCTACTTCA GAGGGAAAAC ATTACTGCTG 
GTGCAGCAAG CGTCCTCCCA GAGCATGACT TACTCTGAAA AGGATGAGAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAGG ACACTCACGC TGCTCAAGTG 
GAGCATGCAC CTGTCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGCATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGCGCACCC 
CAAGTGAGAA GGCCCCTGCA CCCGGCCTTG AACCAGCCAG GCAGCCTCCA GCCCTTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATTCCGG CAATGCCAAA GGCCTCTGTG 
GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAGGAT 
TTCAAACTCA GCGGGCCCAG CAATAGCAGT ATGGAAGATT TCACCAAACG CAGCACCCAG 
AGCGAGGATT TCTCCAGGCG GCATACTGTG CCAGACAGAC ACATACCTCT TGCTCTGCCA 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCACCATC CCTGCCACAT AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCAGCCCT CATGGCCGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCTTCCT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCGATCCA GAGACAACAA 
ACACAGCAGG TTCAGTCACC TGTGGACTCT GCCACAATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTTC TGAACAATGG ACAATATGAA GAGGATGTGG AAGAAACTGA GCAAAATCAA 
GATGAATCCA AGCATGCTGA GAAGTACGAG CAGGAAATCA CGAAGCTGAA GGAGCGCCTG 
CGAGTGTCCA GCCGGCGGCT GGAGGAGTAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA GTACAAGGCG CGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT CATGGCTGTG 
GAAGAGGAAC TGAAGAAAGA CCACGCTGAG ATGCAAGCCG TTATTGATGC AAAGCAAAAA 
ATAATTGATG CACAGACTAA AGTTGCCACT GTATTTGACA TGTTACCACC TTACTTCGAG 
CATGTATCTG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026930100.1
CDS	309..4463
Translation	MESWKMSIFKRQTSPCPPSYRRSLSEPAYANSEIGTSLKNYPWRGPVKSKRKKNEHAVSNLGKPLGAYRWQTHPSFRWKGGKRERWQTNKGDLDEPQQKVVPKMVGNNASSSANEVLGTSTEKLNLVNTRSLRVPFTRSISEPEPRCSIRFGRPWPTSVRSEESEQQGYFIRGIFSTLSNGFSRMLSLRGESTNEPVREDVKGPPTHRLSCGQSPYTETTTWERRYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRARGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVEPSRCSSSELIEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHAPVMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALMAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQTKVATVFDMLPPYFEHVSG

Target ORF information:

RefSeq Version	XM_027074299.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027074299.1

ORF Insert Sequence:

ATGGAGTCGT GGAAGATGAG CATCTTTAAG AGACAAACGT CACCTTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA AATTCAGAGA TAGGAACAAG CTTAAAGAAT 
TATCCTTGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACATGC TGTTTCCAAC 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACATC CCAGCTTTAG GTGGAAAGGG 
GGCAAAAGGG AGAGATGGCA GACTAATAAA GGAGACTTGG ATGAACCACA ACAAAAGGTC 
GTGCCAAAGA TGGTTGGTAA TAATGCTTCT TCCTCTGCTA ATGAGGTCCT TGGAACATCA 
ACAGAGAAGC TGAACCTGGT CAACACCCGG TCACTTCGGG TTCCATTCAC CAGATCTATT 
TCCGAGCCAG AACCCCGCTG CAGTATCAGA TTTGGTAGAC CGTGGCCGAC TTCAGTGAGG 
TCAGAGGAGT CTGAGCAGCA GGGATATTTC ATCCGTGGCA TTTTTTCTAC TCTTTCCAAT 
GGCTTCTCCA GGATGCTGAG TTTAAGAGGA GAATCAACCA ATGAGCCAGT AAGAGAAGAT 
GTGAAGGGGC CACCCACGCA TCGTCTGTCT TGCGGCCAGT CACCCTACAC GGAAACGACA 
ACGTGGGAGC GGAGGTACTG CATCCTCACG GACAGCCAGC TGGTGCTGCT CAACAAGGAG 
AAGGAGATAC CTGTGGAGGG AGGACAGGAG CAGCAGACAG ATTCCACCAA AGGGCGATGC 
CTGAGGAGAA CCGTCAGTGT CCCTTCCGAG GGCCAGTTTC CTGAGTACCC ACCAGAGGGC 
GCCACTAAAC TGGAGGTGCC GGCAGAAAGG TCCCCCCGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGTAACA CAGATGACAG GGCTCGTGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCCTTGC TGAGCCCTTG CAGTGCAGTA 
GAGTGTCTGG ATCTTGGCAG AGGAGAGCCC GTGTCAGTGA AACCACTCCA TAGTAGCATC 
CTTGGACAAG ATTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCCGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGCG AACATTTTGA ATTCTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTACGT CGGCCTAGTC AACATTCCCA CTGCCAGTGT GACTGGTCGC 
CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACCCCTACGC CCAACAAAGG AAAGACAGGC 
GGACCTTCTA TTCGGATTAA ATCACGGTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA 
AGTACCGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
CTGTACGAGT CAGATGAGAA CTGTGAGGTG GAGCCCAGCA GGTGTTCCTC TAGTGAACTG 
ATAGAGCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCGTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CGTCATGGAA GCAGCAGTGC 
CTGAACCGTG GCAAGCAGGA CATCAGCGAG CGGCTCATCA GTGCCTCGTT ATTTCTGCGT 
TTTCTGTGCC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TGATGCAGGA GTATCCTGAT 
GACCGCACAT CTCGGACTCT GACTCTAATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAATTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CGGACACCAT CTCGAACACG 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG 
TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CTCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTGAC TAATCCTACA 
CCAATACAAC AGCAACTAAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTC GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACTGAT AGCTACTTCA GAGGGAAAAC ATTACTGCTG 
GTGCAGCAAG CGTCCTCCCA GAGCATGACT TACTCTGAAA AGGATGAGAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAGG ACACTCACGC TGCTCAAGTG 
GAGCATGCAC CTGTCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGCATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGCGCACCC 
CAAGTGAGAA GGCCCCTGCA CCCGGCCTTG AACCAGCCAG GCAGCCTCCA GCCCTTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATTCCGG CAATGCCAAA GGCCTCTGTG 
GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAGGAT 
TTCAAACTCA GCGGGCCCAG CAATAGCAGT ATGGAAGATT TCACCAAACG CAGCACCCAG 
AGCGAGGATT TCTCCAGGCG GCATACTGTG CCAGACAGAC ACATACCTCT TGCTCTGCCA 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCACCATC CCTGCCACAT AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCAGCCCT CATGGCCGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCTTCCT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCGATCCA GAGACAACAA 
ACACAGCAGG TTCAGTCACC TGTGGACTCT GCCACAATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTTC TGAACAATGG ACAATATGAA GAGGATGTGG AAGAAACTGA GCAAAATCAA 
GATGAATCCA AGCATGCTGA GAAGTACGAG CAGGAAATCA CGAAGCTGAA GGAGCGCCTG 
CGAGTGTCCA GCCGGCGGCT GGAGGAGTAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA GTACAAGGCG CGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT CATGGCTGTG 
GAAGAGGAAC TGAAGAAAGA CCACGCTGAG ATGCAAGCCG TTATTGATGC AAAGCAAAAA 
ATAATTGATG CACAGACTAA AGTTGCCACT GTATTTGACA TGTTACCACC TTACTTCGAG 
CATGTATCTG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_027074300.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026930101.1 Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OAc231111
Clone ID Related Accession (Same CDS sequence)	XM_027074300.1
Accession Version	XM_027074300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	ras GTPase-activating protein nGAP isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834759.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT CCCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC GGAGATGTTC 
CCCGGGCTGG AGTCCGACTC GCCGCTGCCT CCGGAGGAGC TGGAAGCGGT TGTCCCAGTC 
GGCGGGGCCG TGGCTGGAGG CATGTTGGAT CGGATCCTGC TGGAGTCCGT GTGCCAGCAA 
CAGAGCTGGG TCCGGGTGTA CGATGTGAAG GGGCCACCCA CGCATCGTCT GTCTTGCGGC 
CAGTCACCCT ACACGGAAAC GACAACGTGG GAGCGGAGGT ACTGCATCCT CACGGACAGC 
CAGCTGGTGC TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA GGAGCAGCAG 
ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACCGTCA GTGTCCCTTC CGAGGGCCAG 
TTTCCTGAGT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCGGCAGA AAGGTCCCCC 
CGCAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGTA 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACAGA AACACGAGCT TTCGGCTTCC ATCCCTTCGT 
AACACAGATG ACAGGGCTCG TGGTCTGCCT AAACTAAAAG AGTCACGTTC CCATGAGTCC 
TTGCTGAGCC CTTGCAGTGC AGTAGAGTGT CTGGATCTTG GCAGAGGAGA GCCCGTGTCA 
GTGAAACCAC TCCATAGTAG CATCCTTGGA CAAGATTTCT GCTTTGAGGT TACCTACTTA 
AGTGGAAGTA AATGCTTCAG CTGTAACTCC GCTTCAGAGA GAGATAAGTG GATGGAAAAC 
CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGCAGAC GAGCTGAAAA TGTTCTTCGT 
TTATGGATCA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGATAA TATTTTCTGG 
GGCGAACATT TTGAATTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA AAAAAAAGAC AAGAATAATT ACGTCGGCCT AGTCAACATT 
CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACCCCT 
ACGCCCAACA AAGGAAAGAC AGGCGGACCT TCTATTCGGA TTAAATCACG GTTCCAAACT 
ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAC 
ACCATGCTGT GTTCTGTCCT TGAACCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCT 
TGTGCCTTAG TGCACATTCT TCAAAGTACC GGCAGAGCCA AGGATTTTCT GACCGACTTG 
GTGATGTCTG AGGTGGATCG CTGTGGAGAG CATGATGTCT TGATCTTCAG AGAGAACACC 
ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC 
GCATTGGGGG AGTTCATCAA AGCTCTGTAC GAGTCAGATG AGAACTGTGA GGTGGAGCCC 
AGCAGGTGTT CCTCTAGTGA ACTGATAGAG CATCAGAGCA ACCTGAAAAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCGTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG 
TTTGCGTCAT GGAAGCAGCA GTGCCTGAAC CGTGGCAAGC AGGACATCAG CGAGCGGCTC 
ATCAGTGCCT CGTTATTTCT GCGTTTTCTG TGCCCAGCCA TTATGTCTCC CAGTCTTTTC 
AACCTGATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT AATTGCCAAG 
GTCATTCAGA ACCTGGCTAA CTTTGCCAAA TTTGGTAACA AAGAAGAATA TATGGCATTC 
ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT 
AATCCGGACA CCATCTCGAA CACGCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT 
TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCTCTCC CTCGAGTTCT TGCTGATATT 
ACCAAGTCTC TGACTAATCC TACACCAATA CAACAGCAAC TAAGACGCTT CACTGAGCAT 
AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTCGAAGAC 
CCCACTGACA GTGATTTGCA TAAACTAAAA TCTCCAAGCC AGGACAACAC TGATAGCTAC 
TTCAGAGGGA AAACATTACT GCTGGTGCAG CAAGCGTCCT CCCAGAGCAT GACTTACTCT 
GAAAAGGATG AGAAGGAAAG TAGCCTTCCT AACGGTCGGA GCATCTCCCT CATGGACCTT 
CAGGACACTC ACGCTGCTCA AGTGGAGCAT GCACCTGTCA TGCTCGATGT GCCTATGCGC 
TTAACTGGAA GCCAGCTTTC CATAACCCAG GTGGCCAGCA TCAAACAACT GCGGGAAACC 
CAGAGCACTC CCCAGAGCGC ACCCCAAGTG AGAAGGCCCC TGCACCCGGC CTTGAACCAG 
CCAGGCAGCC TCCAGCCCTT GTCATTCCAG AACCCTGTCT ATCACCTCAA TAACCCAATT 
CCGGCAATGC CAAAGGCCTC TGTGGATTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC 
AGAAGCCAAA GTAACAGTGA GGATTTCAAA CTCAGCGGGC CCAGCAATAG CAGTATGGAA 
GATTTCACCA AACGCAGCAC CCAGAGCGAG GATTTCTCCA GGCGGCATAC TGTGCCAGAC 
AGACACATAC CTCTTGCTCT GCCACGGCAG AATAGTACCG GGCAGGCCCA GATCCGGAAA 
ATGGACCAGG CTGGGTTAGG TGCCCGAGCC AAAGCCCCAC CATCCCTGCC ACATAGTGCT 
TCCTTACGTA GCACGGGGAG CATGTCGGTG GCCTCTGCAG CCCTCATGGC CGAACCTGTG 
CAGAATGGGA GCCGGTCCCG GCAGCAGTCT TCCTCCTCCA GAGAGAGCCC TGTTCCCAAA 
GTGAGAGCGA TCCAGAGACA ACAAACACAG CAGGTTCAGT CACCTGTGGA CTCTGCCACA 
ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGACAATA TGAAGAGGAT 
GTGGAAGAAA CTGAGCAAAA TCAAGATGAA TCCAAGCATG CTGAGAAGTA CGAGCAGGAA 
ATCACGAAGC TGAAGGAGCG CCTGCGAGTG TCCAGCCGGC GGCTGGAGGA GTATGAGCGC 
CGCTTGCTGG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCGCGGCTG 
GAGGACAGCG AGGAGCGCCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAAAGC 
ATCATCAGCA GGCTCATGGC TGTGGAAGAG GAACTGAAGA AAGACCACGC TGAGATGCAA 
GCCGTTATTG ATGCAAAGCA AAAAATAATT GATGCACAGG TCATAAATGG AGATGAGATT 
ATTCAAACAG GAAAAACGGA TCGTGTCCCT GGATTCGGCC AACACCAGAC TGATGAGCGC 
ACTGACCCAA GTGAAGGAGC GATACAGTGT GCAGGTCCGC AATGGCATCT CCCCCACCAA 
CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026930101.1
CDS	112..3984
Translation	MELSPSSGGAAEALSWPEMFPGLESDSPLPPEELEAVVPVGGAVAGGMLDRILLESVCQQQSWVRVYDVKGPPTHRLSCGQSPYTETTTWERRYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRARGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVEPSRCSSSELIEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHAPVMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALMAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTDRVPGFGQHQTDERTDPSEGAIQCAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_027074300.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027074300.1

ORF Insert Sequence:

ATGGAGCTCT CCCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC GGAGATGTTC 
CCCGGGCTGG AGTCCGACTC GCCGCTGCCT CCGGAGGAGC TGGAAGCGGT TGTCCCAGTC 
GGCGGGGCCG TGGCTGGAGG CATGTTGGAT CGGATCCTGC TGGAGTCCGT GTGCCAGCAA 
CAGAGCTGGG TCCGGGTGTA CGATGTGAAG GGGCCACCCA CGCATCGTCT GTCTTGCGGC 
CAGTCACCCT ACACGGAAAC GACAACGTGG GAGCGGAGGT ACTGCATCCT CACGGACAGC 
CAGCTGGTGC TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA GGAGCAGCAG 
ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACCGTCA GTGTCCCTTC CGAGGGCCAG 
TTTCCTGAGT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCGGCAGA AAGGTCCCCC 
CGCAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGTA 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACAGA AACACGAGCT TTCGGCTTCC ATCCCTTCGT 
AACACAGATG ACAGGGCTCG TGGTCTGCCT AAACTAAAAG AGTCACGTTC CCATGAGTCC 
TTGCTGAGCC CTTGCAGTGC AGTAGAGTGT CTGGATCTTG GCAGAGGAGA GCCCGTGTCA 
GTGAAACCAC TCCATAGTAG CATCCTTGGA CAAGATTTCT GCTTTGAGGT TACCTACTTA 
AGTGGAAGTA AATGCTTCAG CTGTAACTCC GCTTCAGAGA GAGATAAGTG GATGGAAAAC 
CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGCAGAC GAGCTGAAAA TGTTCTTCGT 
TTATGGATCA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGATAA TATTTTCTGG 
GGCGAACATT TTGAATTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA AAAAAAAGAC AAGAATAATT ACGTCGGCCT AGTCAACATT 
CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACCCCT 
ACGCCCAACA AAGGAAAGAC AGGCGGACCT TCTATTCGGA TTAAATCACG GTTCCAAACT 
ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAC 
ACCATGCTGT GTTCTGTCCT TGAACCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCT 
TGTGCCTTAG TGCACATTCT TCAAAGTACC GGCAGAGCCA AGGATTTTCT GACCGACTTG 
GTGATGTCTG AGGTGGATCG CTGTGGAGAG CATGATGTCT TGATCTTCAG AGAGAACACC 
ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC 
GCATTGGGGG AGTTCATCAA AGCTCTGTAC GAGTCAGATG AGAACTGTGA GGTGGAGCCC 
AGCAGGTGTT CCTCTAGTGA ACTGATAGAG CATCAGAGCA ACCTGAAAAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCGTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG 
TTTGCGTCAT GGAAGCAGCA GTGCCTGAAC CGTGGCAAGC AGGACATCAG CGAGCGGCTC 
ATCAGTGCCT CGTTATTTCT GCGTTTTCTG TGCCCAGCCA TTATGTCTCC CAGTCTTTTC 
AACCTGATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT AATTGCCAAG 
GTCATTCAGA ACCTGGCTAA CTTTGCCAAA TTTGGTAACA AAGAAGAATA TATGGCATTC 
ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT 
AATCCGGACA CCATCTCGAA CACGCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT 
TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCTCTCC CTCGAGTTCT TGCTGATATT 
ACCAAGTCTC TGACTAATCC TACACCAATA CAACAGCAAC TAAGACGCTT CACTGAGCAT 
AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTCGAAGAC 
CCCACTGACA GTGATTTGCA TAAACTAAAA TCTCCAAGCC AGGACAACAC TGATAGCTAC 
TTCAGAGGGA AAACATTACT GCTGGTGCAG CAAGCGTCCT CCCAGAGCAT GACTTACTCT 
GAAAAGGATG AGAAGGAAAG TAGCCTTCCT AACGGTCGGA GCATCTCCCT CATGGACCTT 
CAGGACACTC ACGCTGCTCA AGTGGAGCAT GCACCTGTCA TGCTCGATGT GCCTATGCGC 
TTAACTGGAA GCCAGCTTTC CATAACCCAG GTGGCCAGCA TCAAACAACT GCGGGAAACC 
CAGAGCACTC CCCAGAGCGC ACCCCAAGTG AGAAGGCCCC TGCACCCGGC CTTGAACCAG 
CCAGGCAGCC TCCAGCCCTT GTCATTCCAG AACCCTGTCT ATCACCTCAA TAACCCAATT 
CCGGCAATGC CAAAGGCCTC TGTGGATTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC 
AGAAGCCAAA GTAACAGTGA GGATTTCAAA CTCAGCGGGC CCAGCAATAG CAGTATGGAA 
GATTTCACCA AACGCAGCAC CCAGAGCGAG GATTTCTCCA GGCGGCATAC TGTGCCAGAC 
AGACACATAC CTCTTGCTCT GCCACGGCAG AATAGTACCG GGCAGGCCCA GATCCGGAAA 
ATGGACCAGG CTGGGTTAGG TGCCCGAGCC AAAGCCCCAC CATCCCTGCC ACATAGTGCT 
TCCTTACGTA GCACGGGGAG CATGTCGGTG GCCTCTGCAG CCCTCATGGC CGAACCTGTG 
CAGAATGGGA GCCGGTCCCG GCAGCAGTCT TCCTCCTCCA GAGAGAGCCC TGTTCCCAAA 
GTGAGAGCGA TCCAGAGACA ACAAACACAG CAGGTTCAGT CACCTGTGGA CTCTGCCACA 
ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGACAATA TGAAGAGGAT 
GTGGAAGAAA CTGAGCAAAA TCAAGATGAA TCCAAGCATG CTGAGAAGTA CGAGCAGGAA 
ATCACGAAGC TGAAGGAGCG CCTGCGAGTG TCCAGCCGGC GGCTGGAGGA GTATGAGCGC 
CGCTTGCTGG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCGCGGCTG 
GAGGACAGCG AGGAGCGCCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAAAGC 
ATCATCAGCA GGCTCATGGC TGTGGAAGAG GAACTGAAGA AAGACCACGC TGAGATGCAA 
GCCGTTATTG ATGCAAAGCA AAAAATAATT GATGCACAGG TCATAAATGG AGATGAGATT 
ATTCAAACAG GAAAAACGGA TCGTGTCCCT GGATTCGGCC AACACCAGAC TGATGAGCGC 
ACTGACCCAA GTGAAGGAGC GATACAGTGT GCAGGTCCGC AATGGCATCT CCCCCACCAA 
CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_027074301.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026930102.1 Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

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CloneID	OAc231112
Clone ID Related Accession (Same CDS sequence)	XM_027074301.1
Accession Version	XM_027074301.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	ras GTPase-activating protein nGAP isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834759.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT CCCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC GGAGATGTTC 
CCCGGGCTGG AGTCCGACTC GCCGCTGCCT CCGGAGGAGC TGGAAGCGGT TGTCCCAGTC 
GGCGGGGCCG TGGCTGGAGG CATGTTGGAT CGGATCCTGC TGGAGTCCGT GTGCCAGCAA 
CAGAGCTGGG TCCGGGTGTA CGATGTGAAG GGGCCACCCA CGCATCGTCT GTCTTGCGGC 
CAGTCACCCT ACACGGAAAC GACAACGTGG GAGCGGAGGT ACTGCATCCT CACGGACAGC 
CAGCTGGTGC TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA GGAGCAGCAG 
ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACCGTCA GTGTCCCTTC CGAGGGCCAG 
TTTCCTGAGT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCGGCAGA AAGGTCCCCC 
CGCAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGTA 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACAGA AACACGAGCT TTCGGCTTCC ATCCCTTCGT 
AACACAGATG ACAGGGCTCG TGGTCTGCCT AAACTAAAAG AGTCACGTTC CCATGAGTCC 
TTGCTGAGCC CTTGCAGTGC AGTAGAGTGT CTGGATCTTG GCAGAGGAGA GCCCGTGTCA 
GTGAAACCAC TCCATAGTAG CATCCTTGGA CAAGATTTCT GCTTTGAGGT TACCTACTTA 
AGTGGAAGTA AATGCTTCAG CTGTAACTCC GCTTCAGAGA GAGATAAGTG GATGGAAAAC 
CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGCAGAC GAGCTGAAAA TGTTCTTCGT 
TTATGGATCA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGATAA TATTTTCTGG 
GGCGAACATT TTGAATTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA AAAAAAAGAC AAGAATAATT ACGTCGGCCT AGTCAACATT 
CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACCCCT 
ACGCCCAACA AAGGAAAGAC AGGCGGACCT TCTATTCGGA TTAAATCACG GTTCCAAACT 
ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAC 
ACCATGCTGT GTTCTGTCCT TGAACCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCT 
TGTGCCTTAG TGCACATTCT TCAAAGTACC GGCAGAGCCA AGGATTTTCT GACCGACTTG 
GTGATGTCTG AGGTGGATCG CTGTGGAGAG CATGATGTCT TGATCTTCAG AGAGAACACC 
ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC 
GCATTGGGGG AGTTCATCAA AGCTCTGTAC GAGTCAGATG AGAACTGTGA GGTGGAGCCC 
AGCAGGTGTT CCTCTAGTGA ACTGATAGAG CATCAGAGCA ACCTGAAAAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCGTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG 
TTTGCGTCAT GGAAGCAGCA GTGCCTGAAC CGTGGCAAGC AGGACATCAG CGAGCGGCTC 
ATCAGTGCCT CGTTATTTCT GCGTTTTCTG TGCCCAGCCA TTATGTCTCC CAGTCTTTTC 
AACCTGATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT AATTGCCAAG 
GTCATTCAGA ACCTGGCTAA CTTTGCCAAA TTTGGTAACA AAGAAGAATA TATGGCATTC 
ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT 
AATCCGGACA CCATCTCGAA CACGCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT 
TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCTCTCC CTCGAGTTCT TGCTGATATT 
ACCAAGTCTC TGACTAATCC TACACCAATA CAACAGCAAC TAAGACGCTT CACTGAGCAT 
AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTCGAAGAC 
CCCACTGACA GTGATTTGCA TAAACTAAAA TCTCCAAGCC AGGACAACAC TGATAGCTAC 
TTCAGAGGGA AAACATTACT GCTGGTGCAG CAAGCGTCCT CCCAGAGCAT GACTTACTCT 
GAAAAGGATG AGAAGGAAAG TAGCCTTCCT AACGGTCGGA GCATCTCCCT CATGGACCTT 
CAGGACACTC ACGCTGCTCA AGTGGAGCAT GCACCTGTCA TGCTCGATGT GCCTATGCGC 
TTAACTGGAA GCCAGCTTTC CATAACCCAG GTGGCCAGCA TCAAACAACT GCGGGAAACC 
CAGAGCACTC CCCAGAGCGC ACCCCAAGTG AGAAGGCCCC TGCACCCGGC CTTGAACCAG 
CCAGGCAGCC TCCAGCCCTT GTCATTCCAG AACCCTGTCT ATCACCTCAA TAACCCAATT 
CCGGCAATGC CAAAGGCCTC TGTGGATTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC 
AGAAGCCAAA GTAACAGTGA GGATTTCAAA CTCAGCGGGC CCAGCAATAG CAGTATGGAA 
GATTTCACCA AACGCAGCAC CCAGAGCGAG GATTTCTCCA GGCGGCATAC TGTGCCAGAC 
AGACACATAC CTCTTGCTCT GCCACGGCAG AATAGTACCG GGCAGGCCCA GATCCGGAAA 
ATGGACCAGG CTGGGTTAGG TGCCCGAGCC AAAGCCCCAC CATCCCTGCC ACATAGTGCT 
TCCTTACGTA GCACGGGGAG CATGTCGGTG GCCTCTGCAG CCCTCATGGC CGAACCTGTG 
CAGAATGGGA GCCGGTCCCG GCAGCAGTCT TCCTCCTCCA GAGAGAGCCC TGTTCCCAAA 
GTGAGAGCGA TCCAGAGACA ACAAACACAG CAGGTTCAGT CACCTGTGGA CTCTGCCACA 
ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGACAATA TGAAGAGGAT 
GTGGAAGAAA CTGAGCAAAA TCAAGATGAA TCCAAGCATG CTGAGAAGTA CGAGCAGGAA 
ATCACGAAGC TGAAGGAGCG CCTGCGAGTG TCCAGCCGGC GGCTGGAGGA GTATGAGCGC 
CGCTTGCTGG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCGCGGCTG 
GAGGACAGCG AGGAGCGCCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAAAGC 
ATCATCAGCA GGCTCATGGC TGTGGAAGAG GAACTGAAGA AAGACCACGC TGAGATGCAA 
GCCGTTATTG ATGCAAAGCA AAAAATAATT GATGCACAGG AAAAACGGAT CGTGTCCCTG 
GATTCGGCCA ACACCAGACT GATGAGCGCA CTGACCCAAG TGAAGGAGCG ATACAGTGTG 
CAGGTCCGCA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT 
GAATTCAAAA ACAGCAGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026930102.1
CDS	111..3974
Translation	MELSPSSGGAAEALSWPEMFPGLESDSPLPPEELEAVVPVGGAVAGGMLDRILLESVCQQQSWVRVYDVKGPPTHRLSCGQSPYTETTTWERRYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRARGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVEPSRCSSSELIEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHAPVMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALMAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSVQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_027074301.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027074301.1

ORF Insert Sequence:

ATGGAGCTCT CCCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC GGAGATGTTC 
CCCGGGCTGG AGTCCGACTC GCCGCTGCCT CCGGAGGAGC TGGAAGCGGT TGTCCCAGTC 
GGCGGGGCCG TGGCTGGAGG CATGTTGGAT CGGATCCTGC TGGAGTCCGT GTGCCAGCAA 
CAGAGCTGGG TCCGGGTGTA CGATGTGAAG GGGCCACCCA CGCATCGTCT GTCTTGCGGC 
CAGTCACCCT ACACGGAAAC GACAACGTGG GAGCGGAGGT ACTGCATCCT CACGGACAGC 
CAGCTGGTGC TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA GGAGCAGCAG 
ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACCGTCA GTGTCCCTTC CGAGGGCCAG 
TTTCCTGAGT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCGGCAGA AAGGTCCCCC 
CGCAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGTA 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACAGA AACACGAGCT TTCGGCTTCC ATCCCTTCGT 
AACACAGATG ACAGGGCTCG TGGTCTGCCT AAACTAAAAG AGTCACGTTC CCATGAGTCC 
TTGCTGAGCC CTTGCAGTGC AGTAGAGTGT CTGGATCTTG GCAGAGGAGA GCCCGTGTCA 
GTGAAACCAC TCCATAGTAG CATCCTTGGA CAAGATTTCT GCTTTGAGGT TACCTACTTA 
AGTGGAAGTA AATGCTTCAG CTGTAACTCC GCTTCAGAGA GAGATAAGTG GATGGAAAAC 
CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGCAGAC GAGCTGAAAA TGTTCTTCGT 
TTATGGATCA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGATAA TATTTTCTGG 
GGCGAACATT TTGAATTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA AAAAAAAGAC AAGAATAATT ACGTCGGCCT AGTCAACATT 
CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACCCCT 
ACGCCCAACA AAGGAAAGAC AGGCGGACCT TCTATTCGGA TTAAATCACG GTTCCAAACT 
ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAC 
ACCATGCTGT GTTCTGTCCT TGAACCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCT 
TGTGCCTTAG TGCACATTCT TCAAAGTACC GGCAGAGCCA AGGATTTTCT GACCGACTTG 
GTGATGTCTG AGGTGGATCG CTGTGGAGAG CATGATGTCT TGATCTTCAG AGAGAACACC 
ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC 
GCATTGGGGG AGTTCATCAA AGCTCTGTAC GAGTCAGATG AGAACTGTGA GGTGGAGCCC 
AGCAGGTGTT CCTCTAGTGA ACTGATAGAG CATCAGAGCA ACCTGAAAAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCGTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG 
TTTGCGTCAT GGAAGCAGCA GTGCCTGAAC CGTGGCAAGC AGGACATCAG CGAGCGGCTC 
ATCAGTGCCT CGTTATTTCT GCGTTTTCTG TGCCCAGCCA TTATGTCTCC CAGTCTTTTC 
AACCTGATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT AATTGCCAAG 
GTCATTCAGA ACCTGGCTAA CTTTGCCAAA TTTGGTAACA AAGAAGAATA TATGGCATTC 
ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT 
AATCCGGACA CCATCTCGAA CACGCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT 
TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGACCTCTCC CTCGAGTTCT TGCTGATATT 
ACCAAGTCTC TGACTAATCC TACACCAATA CAACAGCAAC TAAGACGCTT CACTGAGCAT 
AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTCGAAGAC 
CCCACTGACA GTGATTTGCA TAAACTAAAA TCTCCAAGCC AGGACAACAC TGATAGCTAC 
TTCAGAGGGA AAACATTACT GCTGGTGCAG CAAGCGTCCT CCCAGAGCAT GACTTACTCT 
GAAAAGGATG AGAAGGAAAG TAGCCTTCCT AACGGTCGGA GCATCTCCCT CATGGACCTT 
CAGGACACTC ACGCTGCTCA AGTGGAGCAT GCACCTGTCA TGCTCGATGT GCCTATGCGC 
TTAACTGGAA GCCAGCTTTC CATAACCCAG GTGGCCAGCA TCAAACAACT GCGGGAAACC 
CAGAGCACTC CCCAGAGCGC ACCCCAAGTG AGAAGGCCCC TGCACCCGGC CTTGAACCAG 
CCAGGCAGCC TCCAGCCCTT GTCATTCCAG AACCCTGTCT ATCACCTCAA TAACCCAATT 
CCGGCAATGC CAAAGGCCTC TGTGGATTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC 
AGAAGCCAAA GTAACAGTGA GGATTTCAAA CTCAGCGGGC CCAGCAATAG CAGTATGGAA 
GATTTCACCA AACGCAGCAC CCAGAGCGAG GATTTCTCCA GGCGGCATAC TGTGCCAGAC 
AGACACATAC CTCTTGCTCT GCCACGGCAG AATAGTACCG GGCAGGCCCA GATCCGGAAA 
ATGGACCAGG CTGGGTTAGG TGCCCGAGCC AAAGCCCCAC CATCCCTGCC ACATAGTGCT 
TCCTTACGTA GCACGGGGAG CATGTCGGTG GCCTCTGCAG CCCTCATGGC CGAACCTGTG 
CAGAATGGGA GCCGGTCCCG GCAGCAGTCT TCCTCCTCCA GAGAGAGCCC TGTTCCCAAA 
GTGAGAGCGA TCCAGAGACA ACAAACACAG CAGGTTCAGT CACCTGTGGA CTCTGCCACA 
ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGACAATA TGAAGAGGAT 
GTGGAAGAAA CTGAGCAAAA TCAAGATGAA TCCAAGCATG CTGAGAAGTA CGAGCAGGAA 
ATCACGAAGC TGAAGGAGCG CCTGCGAGTG TCCAGCCGGC GGCTGGAGGA GTATGAGCGC 
CGCTTGCTGG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCGCGGCTG 
GAGGACAGCG AGGAGCGCCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAAAGC 
ATCATCAGCA GGCTCATGGC TGTGGAAGAG GAACTGAAGA AAGACCACGC TGAGATGCAA 
GCCGTTATTG ATGCAAAGCA AAAAATAATT GATGCACAGG AAAAACGGAT CGTGTCCCTG 
GATTCGGCCA ACACCAGACT GATGAGCGCA CTGACCCAAG TGAAGGAGCG ATACAGTGTG 
CAGGTCCGCA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT 
GAATTCAAAA ACAGCAGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_027074302.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026930103.1 Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OAc231113
Clone ID Related Accession (Same CDS sequence)	XM_027074302.1
Accession Version	XM_027074302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	ras GTPase-activating protein nGAP isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834759.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT CCCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC GGAGATGTTC 
CCCGGGCTGG AGTCCGACTC GCCGCTGCCT CCGGAGGAGC TGGAAGCGGT TGTCCCAGTC 
GGCGGGGCCG TGGCTGGAGG CATGTTGGAT CGGATCCTGC TGGAGTCCGT GTGCCAGCAA 
CAGAGCTGGG TCCGGGTGTA CGATGTGAAG GGGCCACCCA CGCATCGTCT GTCTTGCGGC 
CAGTCACCCT ACACGGAAAC GACAACGTGG GAGCGGAGGT ACTGCATCCT CACGGACAGC 
CAGCTGGTGC TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA GGAGCAGCAG 
ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACCGTCA GTGTCCCTTC CGAGGGCCAG 
TTTCCTGAGT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCGGCAGA AAGGTCCCCC 
CGCAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGTA 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACAGA AACACGAGCT TTCGGCTTCC ATCCCTTCGT 
AACACAGATG ACAGGGCTCG TGGTCTGCCT AAACTAAAAG AGTCACGTTC CCATGAGTCC 
TTGCTGAGCC CTTGCAGTGC AGTAGAGTGT CTGGATCTTG GCAGAGGAGA GCCCGTGTCA 
GTGAAACCAC TCCATAGTAG CATCCTTGGA CAAGATTTCT GCTTTGAGGT TACCTACTTA 
AGTGGAAGTA AATGCTTCAG CTGTAACTCC GCTTCAGAGA GAGATAAGTG GATGGAAAAC 
CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGCAGAC GAGCTGAAAA TGTTCTTCGT 
TTATGGATCA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGATAA TATTTTCTGG 
GGCGAACATT TTGAATTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA AAAAAAAGAC AAGAATAATT ACGTCGGCCT AGTCAACATT 
CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACCCCT 
ACGCCCAACA AAGGAAAGAC AGGCGGACCT TCTATTCGGA TTAAATCACG GTTCCAAACT 
ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAC 
ACCATGCTGT GTTCTGTCCT TGAACCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCT 
TGTGCCTTAG TGCACATTCT TCAAAGTACC GGCAGAGCCA AGGATTTTCT GACCGACTTG 
GTGATGTCTG AGGTGGATCG CTGTGGAGAG CATGATGTCT TGATCTTCAG AGAGAACACC 
ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC 
GCATTGGGGG AGTTCATCAA AGCTCTGTAC GAGTCAGATG AGAACTGTGA GGTGGAGCCC 
AGCAGGTGTT CCTCTAGTGA ACTGATAGAG CATCAGAGCA ACCTGAAAAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCGTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG 
TTTGCGTCAT GGAAGCAGCA GTGCCTGAAC CGTGGCAAGC AGGACATCAG CGAGCGGCTC 
ATCAGTGCCT CGTTATTTCT GCGTTTTCTG TGCCCAGCCA TTATGTCTCC CAGTCTTTTC 
AACCTGATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT AATTGCCAAG 
GTCATTCAGA ACCTGGCTAA CTTTGCCAAA TTTGGTAACA AAGAAGAATA TATGGCATTC 
ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT 
AATCCGGACA CCATCTCGAA CACGCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGCGACCGTG 
GCAAAATTGG GACCTCTCCC TCGAGTTCTT GCTGATATTA CCAAGTCTCT GACTAATCCT 
ACACCAATAC AACAGCAACT AAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT 
GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTCGAAGACC CCACTGACAG TGATTTGCAT 
AAACTAAAAT CTCCAAGCCA GGACAACACT GATAGCTACT TCAGAGGGAA AACATTACTG 
CTGGTGCAGC AAGCGTCCTC CCAGAGCATG ACTTACTCTG AAAAGGATGA GAAGGAAAGT 
AGCCTTCCTA ACGGTCGGAG CATCTCCCTC ATGGACCTTC AGGACACTCA CGCTGCTCAA 
GTGGAGCATG CACCTGTCAT GCTCGATGTG CCTATGCGCT TAACTGGAAG CCAGCTTTCC 
ATAACCCAGG TGGCCAGCAT CAAACAACTG CGGGAAACCC AGAGCACTCC CCAGAGCGCA 
CCCCAAGTGA GAAGGCCCCT GCACCCGGCC TTGAACCAGC CAGGCAGCCT CCAGCCCTTG 
TCATTCCAGA ACCCTGTCTA TCACCTCAAT AACCCAATTC CGGCAATGCC AAAGGCCTCT 
GTGGATTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GAAGCCAAAG TAACAGTGAG 
GATTTCAAAC TCAGCGGGCC CAGCAATAGC AGTATGGAAG ATTTCACCAA ACGCAGCACC 
CAGAGCGAGG ATTTCTCCAG GCGGCATACT GTGCCAGACA GACACATACC TCTTGCTCTG 
CCACGGCAGA ATAGTACCGG GCAGGCCCAG ATCCGGAAAA TGGACCAGGC TGGGTTAGGT 
GCCCGAGCCA AAGCCCCACC ATCCCTGCCA CATAGTGCTT CCTTACGTAG CACGGGGAGC 
ATGTCGGTGG CCTCTGCAGC CCTCATGGCC GAACCTGTGC AGAATGGGAG CCGGTCCCGG 
CAGCAGTCTT CCTCCTCCAG AGAGAGCCCT GTTCCCAAAG TGAGAGCGAT CCAGAGACAA 
CAAACACAGC AGGTTCAGTC ACCTGTGGAC TCTGCCACAA TGTCCCCAGT AGAGAGGACA 
GCAGCCTGGG TTCTGAACAA TGGACAATAT GAAGAGGATG TGGAAGAAAC TGAGCAAAAT 
CAAGATGAAT CCAAGCATGC TGAGAAGTAC GAGCAGGAAA TCACGAAGCT GAAGGAGCGC 
CTGCGAGTGT CCAGCCGGCG GCTGGAGGAG TATGAGCGCC GCTTGCTGGT GCAGGAGCAG 
CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCGCGGCTGG AGGACAGCGA GGAGCGCCTG 
CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAAAGCA TCATCAGCAG GCTCATGGCT 
GTGGAAGAGG AACTGAAGAA AGACCACGCT GAGATGCAAG CCGTTATTGA TGCAAAGCAA 
AAAATAATTG ATGCACAGGA AAAACGGATC GTGTCCCTGG ATTCGGCCAA CACCAGACTG 
ATGAGCGCAC TGACCCAAGT GAAGGAGCGA TACAGTGTGC AGGTCCGCAA TGGCATCTCC 
CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026930103.1
CDS	111..3953
Translation	MELSPSSGGAAEALSWPEMFPGLESDSPLPPEELEAVVPVGGAVAGGMLDRILLESVCQQQSWVRVYDVKGPPTHRLSCGQSPYTETTTWERRYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRARGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVEPSRCSSSELIEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHAPVMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALMAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSVQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_027074302.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027074302.1

ORF Insert Sequence:

ATGGAGCTCT CCCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC GGAGATGTTC 
CCCGGGCTGG AGTCCGACTC GCCGCTGCCT CCGGAGGAGC TGGAAGCGGT TGTCCCAGTC 
GGCGGGGCCG TGGCTGGAGG CATGTTGGAT CGGATCCTGC TGGAGTCCGT GTGCCAGCAA 
CAGAGCTGGG TCCGGGTGTA CGATGTGAAG GGGCCACCCA CGCATCGTCT GTCTTGCGGC 
CAGTCACCCT ACACGGAAAC GACAACGTGG GAGCGGAGGT ACTGCATCCT CACGGACAGC 
CAGCTGGTGC TGCTCAACAA GGAGAAGGAG ATACCTGTGG AGGGAGGACA GGAGCAGCAG 
ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACCGTCA GTGTCCCTTC CGAGGGCCAG 
TTTCCTGAGT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCGGCAGA AAGGTCCCCC 
CGCAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGTA 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACAGA AACACGAGCT TTCGGCTTCC ATCCCTTCGT 
AACACAGATG ACAGGGCTCG TGGTCTGCCT AAACTAAAAG AGTCACGTTC CCATGAGTCC 
TTGCTGAGCC CTTGCAGTGC AGTAGAGTGT CTGGATCTTG GCAGAGGAGA GCCCGTGTCA 
GTGAAACCAC TCCATAGTAG CATCCTTGGA CAAGATTTCT GCTTTGAGGT TACCTACTTA 
AGTGGAAGTA AATGCTTCAG CTGTAACTCC GCTTCAGAGA GAGATAAGTG GATGGAAAAC 
CTTCGTAGGA CAGTTCAACC AAATAAGGAC AATTGCAGAC GAGCTGAAAA TGTTCTTCGT 
TTATGGATCA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGATAA TATTTTCTGG 
GGCGAACATT TTGAATTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA AAAAAAAGAC AAGAATAATT ACGTCGGCCT AGTCAACATT 
CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACCCCT 
ACGCCCAACA AAGGAAAGAC AGGCGGACCT TCTATTCGGA TTAAATCACG GTTCCAAACT 
ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAC 
ACCATGCTGT GTTCTGTCCT TGAACCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCT 
TGTGCCTTAG TGCACATTCT TCAAAGTACC GGCAGAGCCA AGGATTTTCT GACCGACTTG 
GTGATGTCTG AGGTGGATCG CTGTGGAGAG CATGATGTCT TGATCTTCAG AGAGAACACC 
ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC 
GCATTGGGGG AGTTCATCAA AGCTCTGTAC GAGTCAGATG AGAACTGTGA GGTGGAGCCC 
AGCAGGTGTT CCTCTAGTGA ACTGATAGAG CATCAGAGCA ACCTGAAAAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCGTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG 
TTTGCGTCAT GGAAGCAGCA GTGCCTGAAC CGTGGCAAGC AGGACATCAG CGAGCGGCTC 
ATCAGTGCCT CGTTATTTCT GCGTTTTCTG TGCCCAGCCA TTATGTCTCC CAGTCTTTTC 
AACCTGATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT AATTGCCAAG 
GTCATTCAGA ACCTGGCTAA CTTTGCCAAA TTTGGTAACA AAGAAGAATA TATGGCATTC 
ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT 
AATCCGGACA CCATCTCGAA CACGCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGCGACCGTG 
GCAAAATTGG GACCTCTCCC TCGAGTTCTT GCTGATATTA CCAAGTCTCT GACTAATCCT 
ACACCAATAC AACAGCAACT AAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT 
GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTCGAAGACC CCACTGACAG TGATTTGCAT 
AAACTAAAAT CTCCAAGCCA GGACAACACT GATAGCTACT TCAGAGGGAA AACATTACTG 
CTGGTGCAGC AAGCGTCCTC CCAGAGCATG ACTTACTCTG AAAAGGATGA GAAGGAAAGT 
AGCCTTCCTA ACGGTCGGAG CATCTCCCTC ATGGACCTTC AGGACACTCA CGCTGCTCAA 
GTGGAGCATG CACCTGTCAT GCTCGATGTG CCTATGCGCT TAACTGGAAG CCAGCTTTCC 
ATAACCCAGG TGGCCAGCAT CAAACAACTG CGGGAAACCC AGAGCACTCC CCAGAGCGCA 
CCCCAAGTGA GAAGGCCCCT GCACCCGGCC TTGAACCAGC CAGGCAGCCT CCAGCCCTTG 
TCATTCCAGA ACCCTGTCTA TCACCTCAAT AACCCAATTC CGGCAATGCC AAAGGCCTCT 
GTGGATTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GAAGCCAAAG TAACAGTGAG 
GATTTCAAAC TCAGCGGGCC CAGCAATAGC AGTATGGAAG ATTTCACCAA ACGCAGCACC 
CAGAGCGAGG ATTTCTCCAG GCGGCATACT GTGCCAGACA GACACATACC TCTTGCTCTG 
CCACGGCAGA ATAGTACCGG GCAGGCCCAG ATCCGGAAAA TGGACCAGGC TGGGTTAGGT 
GCCCGAGCCA AAGCCCCACC ATCCCTGCCA CATAGTGCTT CCTTACGTAG CACGGGGAGC 
ATGTCGGTGG CCTCTGCAGC CCTCATGGCC GAACCTGTGC AGAATGGGAG CCGGTCCCGG 
CAGCAGTCTT CCTCCTCCAG AGAGAGCCCT GTTCCCAAAG TGAGAGCGAT CCAGAGACAA 
CAAACACAGC AGGTTCAGTC ACCTGTGGAC TCTGCCACAA TGTCCCCAGT AGAGAGGACA 
GCAGCCTGGG TTCTGAACAA TGGACAATAT GAAGAGGATG TGGAAGAAAC TGAGCAAAAT 
CAAGATGAAT CCAAGCATGC TGAGAAGTAC GAGCAGGAAA TCACGAAGCT GAAGGAGCGC 
CTGCGAGTGT CCAGCCGGCG GCTGGAGGAG TATGAGCGCC GCTTGCTGGT GCAGGAGCAG 
CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCGCGGCTGG AGGACAGCGA GGAGCGCCTG 
CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAAAGCA TCATCAGCAG GCTCATGGCT 
GTGGAAGAGG AACTGAAGAA AGACCACGCT GAGATGCAAG CCGTTATTGA TGCAAAGCAA 
AAAATAATTG ATGCACAGGA AAAACGGATC GTGTCCCTGG ATTCGGCCAA CACCAGACTG 
ATGAGCGCAC TGACCCAAGT GAAGGAGCGA TACAGTGTGC AGGTCCGCAA TGGCATCTCC 
CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_027074303.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026930104.1 Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OAc231114
Clone ID Related Accession (Same CDS sequence)	XM_027074303.1
Accession Version	XM_027074303.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	ras GTPase-activating protein nGAP isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834759.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGTAA TTGTGCTCTG TATGGAAAAG TTTTCTGGCT GGAGAATGAG TCTCCTTTCC 
CCCGGTGATG TGGCCGAGTG GTTGGATAGT AGCACCGAAG AGGAGAAGAG CCTTCTGTCA 
TTTCTGGAGG TGCCGGCAGA AAGGTCCCCC CGCAGACGGA GTATCTCAGG GACCAGTACA 
TCAGAGAAAC CCAACTCCAT GGACACTGTA AATACCTCAC CCTTCAAAGT ACCAGGGTTC 
TTCAGCAAGC GCCTGAAAGG CTCCATCAAG AGGACCAAAA GCCAATCAAA GCTTGACAGA 
AACACGAGCT TTCGGCTTCC ATCCCTTCGT AACACAGATG ACAGGGCTCG TGGTCTGCCT 
AAACTAAAAG AGTCACGTTC CCATGAGTCC TTGCTGAGCC CTTGCAGTGC AGTAGAGTGT 
CTGGATCTTG GCAGAGGAGA GCCCGTGTCA GTGAAACCAC TCCATAGTAG CATCCTTGGA 
CAAGATTTCT GCTTTGAGGT TACCTACTTA AGTGGAAGTA AATGCTTCAG CTGTAACTCC 
GCTTCAGAGA GAGATAAGTG GATGGAAAAC CTTCGTAGGA CAGTTCAACC AAATAAGGAC 
AATTGCAGAC GAGCTGAAAA TGTTCTTCGT TTATGGATCA TTGAAGCCAA GGACCTTGCC 
CCTAAAAAGA AATATTTCTG TGAACTGTGC CTTGATGATA CCCTCTTTGC TCGTACAACC 
AGCAAGACCA AAGCAGATAA TATTTTCTGG GGCGAACATT TTGAATTCTA CAGCCTTCCA 
CCTCTTCATA GCATCACAGT TCACATTTAT AAAGATGTGG AAAAAAAGAA AAAAAAAGAC 
AAGAATAATT ACGTCGGCCT AGTCAACATT CCCACTGCCA GTGTGACTGG TCGCCAGTTT 
GTAGAAAAGT GGTACCCAGT GAGTACCCCT ACGCCCAACA AAGGAAAGAC AGGCGGACCT 
TCTATTCGGA TTAAATCACG GTTCCAAACT ATCACCATTC TGCCTATGGA GCAATACAAA 
GAATTTGCAG AATTTGTCAC CAGCAACTAC ACCATGCTGT GTTCTGTCCT TGAACCAGTA 
ATTAGTGTGA GAAATAAAGA GGAGTTGGCT TGTGCCTTAG TGCACATTCT TCAAAGTACC 
GGCAGAGCCA AGGATTTTCT GACCGACTTG GTGATGTCTG AGGTGGATCG CTGTGGAGAG 
CATGATGTCT TGATCTTCAG AGAGAACACC ATTGCCACCA AATCCATTGA GGAATACCTC 
AAGTTGGTGG GACAACAGTA TCTTCATGAC GCATTGGGGG AGTTCATCAA AGCTCTGTAC 
GAGTCAGATG AGAACTGTGA GGTGGAGCCC AGCAGGTGTT CCTCTAGTGA ACTGATAGAG 
CATCAGAGCA ACCTGAAAAT GTGCTGTGAG CTGGCTTTCT GCAAGATCAT CAACTCGTAC 
TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG TTTGCGTCAT GGAAGCAGCA GTGCCTGAAC 
CGTGGCAAGC AGGACATCAG CGAGCGGCTC ATCAGTGCCT CGTTATTTCT GCGTTTTCTG 
TGCCCAGCCA TTATGTCTCC CAGTCTTTTC AACCTGATGC AGGAGTATCC TGATGACCGC 
ACATCTCGGA CTCTGACTCT AATTGCCAAG GTCATTCAGA ACCTGGCTAA CTTTGCCAAA 
TTTGGTAACA AAGAAGAATA TATGGCATTC ATGAATGATT TTTTAGAACA TGAATGGGGT 
GGAATGAAGC GCTTTCTTTT GGAGATCTCT AATCCGGACA CCATCTCGAA CACGCCAGGC 
TTTGATGGTT ACATTGATCT GGGCCGAGAG CTTTCAGTTT TGCATTCCTT ACTGTGGGAA 
GTAGTTTCCC AACTTGATAA GGGTGAAAAT TCCTTCCTAC AGGCGACCGT GGCAAAATTG 
GGACCTCTCC CTCGAGTTCT TGCTGATATT ACCAAGTCTC TGACTAATCC TACACCAATA 
CAACAGCAAC TAAGACGCTT CACTGAGCAT AACTCCAGTC CAAATGTCAG TGGAAGCCTC 
TCCTCTGGGC TGCAGAAAAT ATTCGAAGAC CCCACTGACA GTGATTTGCA TAAACTAAAA 
TCTCCAAGCC AGGACAACAC TGATAGCTAC TTCAGAGGGA AAACATTACT GCTGGTGCAG 
CAAGCGTCCT CCCAGAGCAT GACTTACTCT GAAAAGGATG AGAAGGAAAG TAGCCTTCCT 
AACGGTCGGA GCATCTCCCT CATGGACCTT CAGGACACTC ACGCTGCTCA AGTGGAGCAT 
GCACCTGTCA TGCTCGATGT GCCTATGCGC TTAACTGGAA GCCAGCTTTC CATAACCCAG 
GTGGCCAGCA TCAAACAACT GCGGGAAACC CAGAGCACTC CCCAGAGCGC ACCCCAAGTG 
AGAAGGCCCC TGCACCCGGC CTTGAACCAG CCAGGCAGCC TCCAGCCCTT GTCATTCCAG 
AACCCTGTCT ATCACCTCAA TAACCCAATT CCGGCAATGC CAAAGGCCTC TGTGGATTCC 
AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC AGAAGCCAAA GTAACAGTGA GGATTTCAAA 
CTCAGCGGGC CCAGCAATAG CAGTATGGAA GATTTCACCA AACGCAGCAC CCAGAGCGAG 
GATTTCTCCA GGCGGCATAC TGTGCCAGAC AGACACATAC CTCTTGCTCT GCCACGGCAG 
AATAGTACCG GGCAGGCCCA GATCCGGAAA ATGGACCAGG CTGGGTTAGG TGCCCGAGCC 
AAAGCCCCAC CATCCCTGCC ACATAGTGCT TCCTTACGTA GCACGGGGAG CATGTCGGTG 
GCCTCTGCAG CCCTCATGGC CGAACCTGTG CAGAATGGGA GCCGGTCCCG GCAGCAGTCT 
TCCTCCTCCA GAGAGAGCCC TGTTCCCAAA GTGAGAGCGA TCCAGAGACA ACAAACACAG 
CAGGTTCAGT CACCTGTGGA CTCTGCCACA ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG 
GTTCTGAACA ATGGACAATA TGAAGAGGAT GTGGAAGAAA CTGAGCAAAA TCAAGATGAA 
TCCAAGCATG CTGAGAAGTA CGAGCAGGAA ATCACGAAGC TGAAGGAGCG CCTGCGAGTG 
TCCAGCCGGC GGCTGGAGGA GTATGAGCGC CGCTTGCTGG TGCAGGAGCA GCAGATGCAG 
AAGCTGCTGC TGGAGTACAA GGCGCGGCTG GAGGACAGCG AGGAGCGCCT GCGCAGGCAG 
CAGGAGGAGA AGGACAGCCA GATGAAAAGC ATCATCAGCA GGCTCATGGC TGTGGAAGAG 
GAACTGAAGA AAGACCACGC TGAGATGCAA GCCGTTATTG ATGCAAAGCA AAAAATAATT 
GATGCACAGG TCATAAATGG AGATGAGATT ATTCAAACAG GAAAAACGGA TCGTGTCCCT 
GGATTCGGCC AACACCAGAC TGATGAGCGC ACTGACCCAA GTGAAGGAGC GATACAGTGT 
GCAGGTCCGC AATGGCATCT CCCCCACCAA CCCCACCAAG CTTTCCATCA CGGAGAATGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026930104.1
CDS	16..3558
Translation	MRVIVLCMEKFSGWRMSLLSPGDVAEWLDSSTEEEKSLLSFLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRARGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVEPSRCSSSELIEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHAPVMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALMAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTDRVPGFGQHQTDERTDPSEGAIQCAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_027074303.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027074303.1

ORF Insert Sequence:

ATGCGAGTAA TTGTGCTCTG TATGGAAAAG TTTTCTGGCT GGAGAATGAG TCTCCTTTCC 
CCCGGTGATG TGGCCGAGTG GTTGGATAGT AGCACCGAAG AGGAGAAGAG CCTTCTGTCA 
TTTCTGGAGG TGCCGGCAGA AAGGTCCCCC CGCAGACGGA GTATCTCAGG GACCAGTACA 
TCAGAGAAAC CCAACTCCAT GGACACTGTA AATACCTCAC CCTTCAAAGT ACCAGGGTTC 
TTCAGCAAGC GCCTGAAAGG CTCCATCAAG AGGACCAAAA GCCAATCAAA GCTTGACAGA 
AACACGAGCT TTCGGCTTCC ATCCCTTCGT AACACAGATG ACAGGGCTCG TGGTCTGCCT 
AAACTAAAAG AGTCACGTTC CCATGAGTCC TTGCTGAGCC CTTGCAGTGC AGTAGAGTGT 
CTGGATCTTG GCAGAGGAGA GCCCGTGTCA GTGAAACCAC TCCATAGTAG CATCCTTGGA 
CAAGATTTCT GCTTTGAGGT TACCTACTTA AGTGGAAGTA AATGCTTCAG CTGTAACTCC 
GCTTCAGAGA GAGATAAGTG GATGGAAAAC CTTCGTAGGA CAGTTCAACC AAATAAGGAC 
AATTGCAGAC GAGCTGAAAA TGTTCTTCGT TTATGGATCA TTGAAGCCAA GGACCTTGCC 
CCTAAAAAGA AATATTTCTG TGAACTGTGC CTTGATGATA CCCTCTTTGC TCGTACAACC 
AGCAAGACCA AAGCAGATAA TATTTTCTGG GGCGAACATT TTGAATTCTA CAGCCTTCCA 
CCTCTTCATA GCATCACAGT TCACATTTAT AAAGATGTGG AAAAAAAGAA AAAAAAAGAC 
AAGAATAATT ACGTCGGCCT AGTCAACATT CCCACTGCCA GTGTGACTGG TCGCCAGTTT 
GTAGAAAAGT GGTACCCAGT GAGTACCCCT ACGCCCAACA AAGGAAAGAC AGGCGGACCT 
TCTATTCGGA TTAAATCACG GTTCCAAACT ATCACCATTC TGCCTATGGA GCAATACAAA 
GAATTTGCAG AATTTGTCAC CAGCAACTAC ACCATGCTGT GTTCTGTCCT TGAACCAGTA 
ATTAGTGTGA GAAATAAAGA GGAGTTGGCT TGTGCCTTAG TGCACATTCT TCAAAGTACC 
GGCAGAGCCA AGGATTTTCT GACCGACTTG GTGATGTCTG AGGTGGATCG CTGTGGAGAG 
CATGATGTCT TGATCTTCAG AGAGAACACC ATTGCCACCA AATCCATTGA GGAATACCTC 
AAGTTGGTGG GACAACAGTA TCTTCATGAC GCATTGGGGG AGTTCATCAA AGCTCTGTAC 
GAGTCAGATG AGAACTGTGA GGTGGAGCCC AGCAGGTGTT CCTCTAGTGA ACTGATAGAG 
CATCAGAGCA ACCTGAAAAT GTGCTGTGAG CTGGCTTTCT GCAAGATCAT CAACTCGTAC 
TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG TTTGCGTCAT GGAAGCAGCA GTGCCTGAAC 
CGTGGCAAGC AGGACATCAG CGAGCGGCTC ATCAGTGCCT CGTTATTTCT GCGTTTTCTG 
TGCCCAGCCA TTATGTCTCC CAGTCTTTTC AACCTGATGC AGGAGTATCC TGATGACCGC 
ACATCTCGGA CTCTGACTCT AATTGCCAAG GTCATTCAGA ACCTGGCTAA CTTTGCCAAA 
TTTGGTAACA AAGAAGAATA TATGGCATTC ATGAATGATT TTTTAGAACA TGAATGGGGT 
GGAATGAAGC GCTTTCTTTT GGAGATCTCT AATCCGGACA CCATCTCGAA CACGCCAGGC 
TTTGATGGTT ACATTGATCT GGGCCGAGAG CTTTCAGTTT TGCATTCCTT ACTGTGGGAA 
GTAGTTTCCC AACTTGATAA GGGTGAAAAT TCCTTCCTAC AGGCGACCGT GGCAAAATTG 
GGACCTCTCC CTCGAGTTCT TGCTGATATT ACCAAGTCTC TGACTAATCC TACACCAATA 
CAACAGCAAC TAAGACGCTT CACTGAGCAT AACTCCAGTC CAAATGTCAG TGGAAGCCTC 
TCCTCTGGGC TGCAGAAAAT ATTCGAAGAC CCCACTGACA GTGATTTGCA TAAACTAAAA 
TCTCCAAGCC AGGACAACAC TGATAGCTAC TTCAGAGGGA AAACATTACT GCTGGTGCAG 
CAAGCGTCCT CCCAGAGCAT GACTTACTCT GAAAAGGATG AGAAGGAAAG TAGCCTTCCT 
AACGGTCGGA GCATCTCCCT CATGGACCTT CAGGACACTC ACGCTGCTCA AGTGGAGCAT 
GCACCTGTCA TGCTCGATGT GCCTATGCGC TTAACTGGAA GCCAGCTTTC CATAACCCAG 
GTGGCCAGCA TCAAACAACT GCGGGAAACC CAGAGCACTC CCCAGAGCGC ACCCCAAGTG 
AGAAGGCCCC TGCACCCGGC CTTGAACCAG CCAGGCAGCC TCCAGCCCTT GTCATTCCAG 
AACCCTGTCT ATCACCTCAA TAACCCAATT CCGGCAATGC CAAAGGCCTC TGTGGATTCC 
AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC AGAAGCCAAA GTAACAGTGA GGATTTCAAA 
CTCAGCGGGC CCAGCAATAG CAGTATGGAA GATTTCACCA AACGCAGCAC CCAGAGCGAG 
GATTTCTCCA GGCGGCATAC TGTGCCAGAC AGACACATAC CTCTTGCTCT GCCACGGCAG 
AATAGTACCG GGCAGGCCCA GATCCGGAAA ATGGACCAGG CTGGGTTAGG TGCCCGAGCC 
AAAGCCCCAC CATCCCTGCC ACATAGTGCT TCCTTACGTA GCACGGGGAG CATGTCGGTG 
GCCTCTGCAG CCCTCATGGC CGAACCTGTG CAGAATGGGA GCCGGTCCCG GCAGCAGTCT 
TCCTCCTCCA GAGAGAGCCC TGTTCCCAAA GTGAGAGCGA TCCAGAGACA ACAAACACAG 
CAGGTTCAGT CACCTGTGGA CTCTGCCACA ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG 
GTTCTGAACA ATGGACAATA TGAAGAGGAT GTGGAAGAAA CTGAGCAAAA TCAAGATGAA 
TCCAAGCATG CTGAGAAGTA CGAGCAGGAA ATCACGAAGC TGAAGGAGCG CCTGCGAGTG 
TCCAGCCGGC GGCTGGAGGA GTATGAGCGC CGCTTGCTGG TGCAGGAGCA GCAGATGCAG 
AAGCTGCTGC TGGAGTACAA GGCGCGGCTG GAGGACAGCG AGGAGCGCCT GCGCAGGCAG 
CAGGAGGAGA AGGACAGCCA GATGAAAAGC ATCATCAGCA GGCTCATGGC TGTGGAAGAG 
GAACTGAAGA AAGACCACGC TGAGATGCAA GCCGTTATTG ATGCAAAGCA AAAAATAATT 
GATGCACAGG TCATAAATGG AGATGAGATT ATTCAAACAG GAAAAACGGA TCGTGTCCCT 
GGATTCGGCC AACACCAGAC TGATGAGCGC ACTGACCCAA GTGAAGGAGC GATACAGTGT 
GCAGGTCCGC AATGGCATCT CCCCCACCAA CCCCACCAAG CTTTCCATCA CGGAGAATGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_027074304.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026930105.1 Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.

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CloneID	OAc231115
Clone ID Related Accession (Same CDS sequence)	XM_027074304.1
Accession Version	XM_027074304.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	ras GTPase-activating protein nGAP isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834759.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGCA GTTGTGATAA AGAGGTGCCG GCAGAAAGGT CCCCCCGCAG ACGGAGTATC 
TCAGGGACCA GTACATCAGA GAAACCCAAC TCCATGGACA CTGTAAATAC CTCACCCTTC 
AAAGTACCAG GGTTCTTCAG CAAGCGCCTG AAAGGCTCCA TCAAGAGGAC CAAAAGCCAA 
TCAAAGCTTG ACAGAAACAC GAGCTTTCGG CTTCCATCCC TTCGTAACAC AGATGACAGG 
GCTCGTGGTC TGCCTAAACT AAAAGAGTCA CGTTCCCATG AGTCCTTGCT GAGCCCTTGC 
AGTGCAGTAG AGTGTCTGGA TCTTGGCAGA GGAGAGCCCG TGTCAGTGAA ACCACTCCAT 
AGTAGCATCC TTGGACAAGA TTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC 
TTCAGCTGTA ACTCCGCTTC AGAGAGAGAT AAGTGGATGG AAAACCTTCG TAGGACAGTT 
CAACCAAATA AGGACAATTG CAGACGAGCT GAAAATGTTC TTCGTTTATG GATCATTGAA 
GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC 
TTTGCTCGTA CAACCAGCAA GACCAAAGCA GATAATATTT TCTGGGGCGA ACATTTTGAA 
TTCTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA 
AAGAAAAAAA AAGACAAGAA TAATTACGTC GGCCTAGTCA ACATTCCCAC TGCCAGTGTG 
ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGTA CCCCTACGCC CAACAAAGGA 
AAGACAGGCG GACCTTCTAT TCGGATTAAA TCACGGTTCC AAACTATCAC CATTCTGCCT 
ATGGAGCAAT ACAAAGAATT TGCAGAATTT GTCACCAGCA ACTACACCAT GCTGTGTTCT 
GTCCTTGAAC CAGTAATTAG TGTGAGAAAT AAAGAGGAGT TGGCTTGTGC CTTAGTGCAC 
ATTCTTCAAA GTACCGGCAG AGCCAAGGAT TTTCTGACCG ACTTGGTGAT GTCTGAGGTG 
GATCGCTGTG GAGAGCATGA TGTCTTGATC TTCAGAGAGA ACACCATTGC CACCAAATCC 
ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTATCTTC ATGACGCATT GGGGGAGTTC 
ATCAAAGCTC TGTACGAGTC AGATGAGAAC TGTGAGGTGG AGCCCAGCAG GTGTTCCTCT 
AGTGAACTGA TAGAGCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG 
ATCATCAACT CGTACTGTGT TTTCCCTCGT GAGTTGAAAG AAGTGTTTGC GTCATGGAAG 
CAGCAGTGCC TGAACCGTGG CAAGCAGGAC ATCAGCGAGC GGCTCATCAG TGCCTCGTTA 
TTTCTGCGTT TTCTGTGCCC AGCCATTATG TCTCCCAGTC TTTTCAACCT GATGCAGGAG 
TATCCTGATG ACCGCACATC TCGGACTCTG ACTCTAATTG CCAAGGTCAT TCAGAACCTG 
GCTAACTTTG CCAAATTTGG TAACAAAGAA GAATATATGG CATTCATGAA TGATTTTTTA 
GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC GGACACCATC 
TCGAACACGC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT 
TCCTTACTGT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG 
ACCGTGGCAA AATTGGGACC TCTCCCTCGA GTTCTTGCTG ATATTACCAA GTCTCTGACT 
AATCCTACAC CAATACAACA GCAACTAAGA CGCTTCACTG AGCATAACTC CAGTCCAAAT 
GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTCG AAGACCCCAC TGACAGTGAT 
TTGCATAAAC TAAAATCTCC AAGCCAGGAC AACACTGATA GCTACTTCAG AGGGAAAACA 
TTACTGCTGG TGCAGCAAGC GTCCTCCCAG AGCATGACTT ACTCTGAAAA GGATGAGAAG 
GAAAGTAGCC TTCCTAACGG TCGGAGCATC TCCCTCATGG ACCTTCAGGA CACTCACGCT 
GCTCAAGTGG AGCATGCACC TGTCATGCTC GATGTGCCTA TGCGCTTAAC TGGAAGCCAG 
CTTTCCATAA CCCAGGTGGC CAGCATCAAA CAACTGCGGG AAACCCAGAG CACTCCCCAG 
AGCGCACCCC AAGTGAGAAG GCCCCTGCAC CCGGCCTTGA ACCAGCCAGG CAGCCTCCAG 
CCCTTGTCAT TCCAGAACCC TGTCTATCAC CTCAATAACC CAATTCCGGC AATGCCAAAG 
GCCTCTGTGG ATTCCAGTTT GGAGAACCTA AGCACTGCCA GTTCCAGAAG CCAAAGTAAC 
AGTGAGGATT TCAAACTCAG CGGGCCCAGC AATAGCAGTA TGGAAGATTT CACCAAACGC 
AGCACCCAGA GCGAGGATTT CTCCAGGCGG CATACTGTGC CAGACAGACA CATACCTCTT 
GCTCTGCCAC GGCAGAATAG TACCGGGCAG GCCCAGATCC GGAAAATGGA CCAGGCTGGG 
TTAGGTGCCC GAGCCAAAGC CCCACCATCC CTGCCACATA GTGCTTCCTT ACGTAGCACG 
GGGAGCATGT CGGTGGCCTC TGCAGCCCTC ATGGCCGAAC CTGTGCAGAA TGGGAGCCGG 
TCCCGGCAGC AGTCTTCCTC CTCCAGAGAG AGCCCTGTTC CCAAAGTGAG AGCGATCCAG 
AGACAACAAA CACAGCAGGT TCAGTCACCT GTGGACTCTG CCACAATGTC CCCAGTAGAG 
AGGACAGCAG CCTGGGTTCT GAACAATGGA CAATATGAAG AGGATGTGGA AGAAACTGAG 
CAAAATCAAG ATGAATCCAA GCATGCTGAG AAGTACGAGC AGGAAATCAC GAAGCTGAAG 
GAGCGCCTGC GAGTGTCCAG CCGGCGGCTG GAGGAGTATG AGCGCCGCTT GCTGGTGCAG 
GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG TACAAGGCGC GGCTGGAGGA CAGCGAGGAG 
CGCCTGCGCA GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AAAGCATCAT CAGCAGGCTC 
ATGGCTGTGG AAGAGGAACT GAAGAAAGAC CACGCTGAGA TGCAAGCCGT TATTGATGCA 
AAGCAAAAAA TAATTGATGC ACAGGTCATA AATGGAGATG AGATTATTCA AACAGGAAAA 
ACGGATCGTG TCCCTGGATT CGGCCAACAC CAGACTGATG AGCGCACTGA CCCAAGTGAA 
GGAGCGATAC AGTGTGCAGG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC 
CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026930105.1
CDS	174..3611
Translation	MGSSCDKEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRARGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVEPSRCSSSELIEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHAPVMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALMAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTDRVPGFGQHQTDERTDPSEGAIQCAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_027074304.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027074304.1

ORF Insert Sequence:

ATGGGAAGCA GTTGTGATAA AGAGGTGCCG GCAGAAAGGT CCCCCCGCAG ACGGAGTATC 
TCAGGGACCA GTACATCAGA GAAACCCAAC TCCATGGACA CTGTAAATAC CTCACCCTTC 
AAAGTACCAG GGTTCTTCAG CAAGCGCCTG AAAGGCTCCA TCAAGAGGAC CAAAAGCCAA 
TCAAAGCTTG ACAGAAACAC GAGCTTTCGG CTTCCATCCC TTCGTAACAC AGATGACAGG 
GCTCGTGGTC TGCCTAAACT AAAAGAGTCA CGTTCCCATG AGTCCTTGCT GAGCCCTTGC 
AGTGCAGTAG AGTGTCTGGA TCTTGGCAGA GGAGAGCCCG TGTCAGTGAA ACCACTCCAT 
AGTAGCATCC TTGGACAAGA TTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC 
TTCAGCTGTA ACTCCGCTTC AGAGAGAGAT AAGTGGATGG AAAACCTTCG TAGGACAGTT 
CAACCAAATA AGGACAATTG CAGACGAGCT GAAAATGTTC TTCGTTTATG GATCATTGAA 
GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC 
TTTGCTCGTA CAACCAGCAA GACCAAAGCA GATAATATTT TCTGGGGCGA ACATTTTGAA 
TTCTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA 
AAGAAAAAAA AAGACAAGAA TAATTACGTC GGCCTAGTCA ACATTCCCAC TGCCAGTGTG 
ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGTA CCCCTACGCC CAACAAAGGA 
AAGACAGGCG GACCTTCTAT TCGGATTAAA TCACGGTTCC AAACTATCAC CATTCTGCCT 
ATGGAGCAAT ACAAAGAATT TGCAGAATTT GTCACCAGCA ACTACACCAT GCTGTGTTCT 
GTCCTTGAAC CAGTAATTAG TGTGAGAAAT AAAGAGGAGT TGGCTTGTGC CTTAGTGCAC 
ATTCTTCAAA GTACCGGCAG AGCCAAGGAT TTTCTGACCG ACTTGGTGAT GTCTGAGGTG 
GATCGCTGTG GAGAGCATGA TGTCTTGATC TTCAGAGAGA ACACCATTGC CACCAAATCC 
ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTATCTTC ATGACGCATT GGGGGAGTTC 
ATCAAAGCTC TGTACGAGTC AGATGAGAAC TGTGAGGTGG AGCCCAGCAG GTGTTCCTCT 
AGTGAACTGA TAGAGCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG 
ATCATCAACT CGTACTGTGT TTTCCCTCGT GAGTTGAAAG AAGTGTTTGC GTCATGGAAG 
CAGCAGTGCC TGAACCGTGG CAAGCAGGAC ATCAGCGAGC GGCTCATCAG TGCCTCGTTA 
TTTCTGCGTT TTCTGTGCCC AGCCATTATG TCTCCCAGTC TTTTCAACCT GATGCAGGAG 
TATCCTGATG ACCGCACATC TCGGACTCTG ACTCTAATTG CCAAGGTCAT TCAGAACCTG 
GCTAACTTTG CCAAATTTGG TAACAAAGAA GAATATATGG CATTCATGAA TGATTTTTTA 
GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC GGACACCATC 
TCGAACACGC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT 
TCCTTACTGT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG 
ACCGTGGCAA AATTGGGACC TCTCCCTCGA GTTCTTGCTG ATATTACCAA GTCTCTGACT 
AATCCTACAC CAATACAACA GCAACTAAGA CGCTTCACTG AGCATAACTC CAGTCCAAAT 
GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTCG AAGACCCCAC TGACAGTGAT 
TTGCATAAAC TAAAATCTCC AAGCCAGGAC AACACTGATA GCTACTTCAG AGGGAAAACA 
TTACTGCTGG TGCAGCAAGC GTCCTCCCAG AGCATGACTT ACTCTGAAAA GGATGAGAAG 
GAAAGTAGCC TTCCTAACGG TCGGAGCATC TCCCTCATGG ACCTTCAGGA CACTCACGCT 
GCTCAAGTGG AGCATGCACC TGTCATGCTC GATGTGCCTA TGCGCTTAAC TGGAAGCCAG 
CTTTCCATAA CCCAGGTGGC CAGCATCAAA CAACTGCGGG AAACCCAGAG CACTCCCCAG 
AGCGCACCCC AAGTGAGAAG GCCCCTGCAC CCGGCCTTGA ACCAGCCAGG CAGCCTCCAG 
CCCTTGTCAT TCCAGAACCC TGTCTATCAC CTCAATAACC CAATTCCGGC AATGCCAAAG 
GCCTCTGTGG ATTCCAGTTT GGAGAACCTA AGCACTGCCA GTTCCAGAAG CCAAAGTAAC 
AGTGAGGATT TCAAACTCAG CGGGCCCAGC AATAGCAGTA TGGAAGATTT CACCAAACGC 
AGCACCCAGA GCGAGGATTT CTCCAGGCGG CATACTGTGC CAGACAGACA CATACCTCTT 
GCTCTGCCAC GGCAGAATAG TACCGGGCAG GCCCAGATCC GGAAAATGGA CCAGGCTGGG 
TTAGGTGCCC GAGCCAAAGC CCCACCATCC CTGCCACATA GTGCTTCCTT ACGTAGCACG 
GGGAGCATGT CGGTGGCCTC TGCAGCCCTC ATGGCCGAAC CTGTGCAGAA TGGGAGCCGG 
TCCCGGCAGC AGTCTTCCTC CTCCAGAGAG AGCCCTGTTC CCAAAGTGAG AGCGATCCAG 
AGACAACAAA CACAGCAGGT TCAGTCACCT GTGGACTCTG CCACAATGTC CCCAGTAGAG 
AGGACAGCAG CCTGGGTTCT GAACAATGGA CAATATGAAG AGGATGTGGA AGAAACTGAG 
CAAAATCAAG ATGAATCCAA GCATGCTGAG AAGTACGAGC AGGAAATCAC GAAGCTGAAG 
GAGCGCCTGC GAGTGTCCAG CCGGCGGCTG GAGGAGTATG AGCGCCGCTT GCTGGTGCAG 
GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG TACAAGGCGC GGCTGGAGGA CAGCGAGGAG 
CGCCTGCGCA GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AAAGCATCAT CAGCAGGCTC 
ATGGCTGTGG AAGAGGAACT GAAGAAAGAC CACGCTGAGA TGCAAGCCGT TATTGATGCA 
AAGCAAAAAA TAATTGATGC ACAGGTCATA AATGGAGATG AGATTATTCA AACAGGAAAA 
ACGGATCGTG TCCCTGGATT CGGCCAACAC CAGACTGATG AGCGCACTGA CCCAAGTGAA 
GGAGCGATAC AGTGTGCAGG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC 
CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_027074305.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026930106.1 Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OAc231116
Clone ID Related Accession (Same CDS sequence)	XM_027074305.1
Accession Version	XM_027074305.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	ras GTPase-activating protein nGAP isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834759.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCC CAGAGGTGCC GGCAGAAAGG TCCCCCCGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGTAACA CAGATGACAG GGCTCGTGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCCTTGC TGAGCCCTTG CAGTGCAGTA 
GAGTGTCTGG ATCTTGGCAG AGGAGAGCCC GTGTCAGTGA AACCACTCCA TAGTAGCATC 
CTTGGACAAG ATTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCCGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGCG AACATTTTGA ATTCTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTACGT CGGCCTAGTC AACATTCCCA CTGCCAGTGT GACTGGTCGC 
CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACCCCTACGC CCAACAAAGG AAAGACAGGC 
GGACCTTCTA TTCGGATTAA ATCACGGTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA 
AGTACCGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
CTGTACGAGT CAGATGAGAA CTGTGAGGTG GAGCCCAGCA GGTGTTCCTC TAGTGAACTG 
ATAGAGCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCGTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CGTCATGGAA GCAGCAGTGC 
CTGAACCGTG GCAAGCAGGA CATCAGCGAG CGGCTCATCA GTGCCTCGTT ATTTCTGCGT 
TTTCTGTGCC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TGATGCAGGA GTATCCTGAT 
GACCGCACAT CTCGGACTCT GACTCTAATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAATTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CGGACACCAT CTCGAACACG 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG 
TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CTCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTGAC TAATCCTACA 
CCAATACAAC AGCAACTAAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTC GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACTGAT AGCTACTTCA GAGGGAAAAC ATTACTGCTG 
GTGCAGCAAG CGTCCTCCCA GAGCATGACT TACTCTGAAA AGGATGAGAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAGG ACACTCACGC TGCTCAAGTG 
GAGCATGCAC CTGTCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGCATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGCGCACCC 
CAAGTGAGAA GGCCCCTGCA CCCGGCCTTG AACCAGCCAG GCAGCCTCCA GCCCTTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATTCCGG CAATGCCAAA GGCCTCTGTG 
GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAGGAT 
TTCAAACTCA GCGGGCCCAG CAATAGCAGT ATGGAAGATT TCACCAAACG CAGCACCCAG 
AGCGAGGATT TCTCCAGGCG GCATACTGTG CCAGACAGAC ACATACCTCT TGCTCTGCCA 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCACCATC CCTGCCACAT AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCAGCCCT CATGGCCGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCTTCCT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCGATCCA GAGACAACAA 
ACACAGCAGG TTCAGTCACC TGTGGACTCT GCCACAATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTTC TGAACAATGG ACAATATGAA GAGGATGTGG AAGAAACTGA GCAAAATCAA 
GATGAATCCA AGCATGCTGA GAAGTACGAG CAGGAAATCA CGAAGCTGAA GGAGCGCCTG 
CGAGTGTCCA GCCGGCGGCT GGAGGAGTAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA GTACAAGGCG CGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT CATGGCTGTG 
GAAGAGGAAC TGAAGAAAGA CCACGCTGAG ATGCAAGCCG TTATTGATGC AAAGCAAAAA 
ATAATTGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGGATCGT 
GTCCCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCACTG ACCCAAGTGA AGGAGCGATA 
CAGTGTGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026930106.1
CDS	252..3680
Translation	MQTPEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRARGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVEPSRCSSSELIEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHAPVMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALMAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDESKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIIQTGKTDRVPGFGQHQTDERTDPSEGAIQCAGPQWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_027074305.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus RAS protein activator like 2 (RASAL2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027074305.1

ORF Insert Sequence:

ATGCAGACCC CAGAGGTGCC GGCAGAAAGG TCCCCCCGCA GACGGAGTAT CTCAGGGACC 
AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGTAAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGTAACA CAGATGACAG GGCTCGTGGT 
CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAGTCCTTGC TGAGCCCTTG CAGTGCAGTA 
GAGTGTCTGG ATCTTGGCAG AGGAGAGCCC GTGTCAGTGA AACCACTCCA TAGTAGCATC 
CTTGGACAAG ATTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT 
AACTCCGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTTC GTAGGACAGT TCAACCAAAT 
AAGGACAATT GCAGACGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC 
CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGATAATATT TTCTGGGGCG AACATTTTGA ATTCTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAAAAA 
AAAGACAAGA ATAATTACGT CGGCCTAGTC AACATTCCCA CTGCCAGTGT GACTGGTCGC 
CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACCCCTACGC CCAACAAAGG AAAGACAGGC 
GGACCTTCTA TTCGGATTAA ATCACGGTTC CAAACTATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAA 
CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA 
AGTACCGGCA GAGCCAAGGA TTTTCTGACC GACTTGGTGA TGTCTGAGGT GGATCGCTGT 
GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA 
TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAT TGGGGGAGTT CATCAAAGCT 
CTGTACGAGT CAGATGAGAA CTGTGAGGTG GAGCCCAGCA GGTGTTCCTC TAGTGAACTG 
ATAGAGCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCGTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CGTCATGGAA GCAGCAGTGC 
CTGAACCGTG GCAAGCAGGA CATCAGCGAG CGGCTCATCA GTGCCTCGTT ATTTCTGCGT 
TTTCTGTGCC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TGATGCAGGA GTATCCTGAT 
GACCGCACAT CTCGGACTCT GACTCTAATT GCCAAGGTCA TTCAGAACCT GGCTAACTTT 
GCCAAATTTG GTAACAAAGA AGAATATATG GCATTCATGA ATGATTTTTT AGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CGGACACCAT CTCGAACACG 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG 
TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA 
AAATTGGGAC CTCTCCCTCG AGTTCTTGCT GATATTACCA AGTCTCTGAC TAATCCTACA 
CCAATACAAC AGCAACTAAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTC GAAGACCCCA CTGACAGTGA TTTGCATAAA 
CTAAAATCTC CAAGCCAGGA CAACACTGAT AGCTACTTCA GAGGGAAAAC ATTACTGCTG 
GTGCAGCAAG CGTCCTCCCA GAGCATGACT TACTCTGAAA AGGATGAGAA GGAAAGTAGC 
CTTCCTAACG GTCGGAGCAT CTCCCTCATG GACCTTCAGG ACACTCACGC TGCTCAAGTG 
GAGCATGCAC CTGTCATGCT CGATGTGCCT ATGCGCTTAA CTGGAAGCCA GCTTTCCATA 
ACCCAGGTGG CCAGCATCAA ACAACTGCGG GAAACCCAGA GCACTCCCCA GAGCGCACCC 
CAAGTGAGAA GGCCCCTGCA CCCGGCCTTG AACCAGCCAG GCAGCCTCCA GCCCTTGTCA 
TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATTCCGG CAATGCCAAA GGCCTCTGTG 
GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAGGAT 
TTCAAACTCA GCGGGCCCAG CAATAGCAGT ATGGAAGATT TCACCAAACG CAGCACCCAG 
AGCGAGGATT TCTCCAGGCG GCATACTGTG CCAGACAGAC ACATACCTCT TGCTCTGCCA 
CGGCAGAATA GTACCGGGCA GGCCCAGATC CGGAAAATGG ACCAGGCTGG GTTAGGTGCC 
CGAGCCAAAG CCCCACCATC CCTGCCACAT AGTGCTTCCT TACGTAGCAC GGGGAGCATG 
TCGGTGGCCT CTGCAGCCCT CATGGCCGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG 
CAGTCTTCCT CCTCCAGAGA GAGCCCTGTT CCCAAAGTGA GAGCGATCCA GAGACAACAA 
ACACAGCAGG TTCAGTCACC TGTGGACTCT GCCACAATGT CCCCAGTAGA GAGGACAGCA 
GCCTGGGTTC TGAACAATGG ACAATATGAA GAGGATGTGG AAGAAACTGA GCAAAATCAA 
GATGAATCCA AGCATGCTGA GAAGTACGAG CAGGAAATCA CGAAGCTGAA GGAGCGCCTG 
CGAGTGTCCA GCCGGCGGCT GGAGGAGTAT GAGCGCCGCT TGCTGGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA GTACAAGGCG CGGCTGGAGG ACAGCGAGGA GCGCCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAAAGCATCA TCAGCAGGCT CATGGCTGTG 
GAAGAGGAAC TGAAGAAAGA CCACGCTGAG ATGCAAGCCG TTATTGATGC AAAGCAAAAA 
ATAATTGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGGATCGT 
GTCCCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCACTG ACCCAAGTGA AGGAGCGATA 
CAGTGTGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_015083312.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_014938798.1 Acinonyx jubatus RAS protein activator like 2 (RASAL2), mRNA.

Price & Availability↑

CloneID	OAc54184
Clone ID Related Accession (Same CDS sequence)	XM_015083312.1
Accession Version	XM_015083312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Acinonyx jubatus(cheetah)
Product	LOW QUALITY PROTEIN: ras GTPase-activating protein nGAP
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015131519.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acinonyx jubatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCTGAGTT TAAGAGGAGA ATCAACCAAT GAGCCAGTAA GAGAAGATGT GAAGGGGCCA 
CCCACGCATC GTCTGTCTTG CGGCCAGTCA CCCTACACGG AAACGACAAC GTGGGAGCGG 
AGGTACTGCA TCCTCACGGA CAGCCAGCTG GTGCTGCTCA ACAAGGAGAA GGAGATACCT 
GTGGAGGGAG GACAGGAGCA GCAGACAGAT TCCACCAAAG GGCGATGCCT GAGGAGAACC 
GTCAGTGTCC CTTCCGAGGG CCAGTTTCCT GAGTACCCAC CAGAGGGCGC CACTAAACTG 
GAGGTGCCGG CAGAAAGGTC CCCCCGCAGA CGGAGTATCT CAGGGACCAG TACATCAGAG 
AAACCCAACT CCATGGACAC TGTAAATACC TCACCCTTCA AAGTACCAGG GTTCTTCAGC 
AAGCGCCTGA AAGGCTCCAT CAAGAGGACC AAAAGCCAAT CAAAGCTTGA CAGAAACACG 
AGCTTTCGGC TTCCATCCCT TCGTAACACA GATGACAGGG CTCGTGGTCT GCCTAAACTA 
AAAGAGTCAC GTTCCCATGA GTCCTTGCTG AGCCCTTGCA GTGCAGTAGA GTGTCTGGAT 
CTTGGCAGAG GAGAGCCCGT GTCAGTGAAA CCACTCCATA GTAGCATCCT TGGACAAGAT 
TTCTGCTTTG AGGTTACCTA CTTAAGTGGA AGTAAATGCT TCAGCTGTAA CTCCGCTTCA 
GAGAGAGATA AGTGGATGGA AAACCTTCGT AGGACAGTTC AACCAAATAA GGACAATTGC 
AGACGAGCTG AAAATGTTCT TCGTTTATGG ATCATTGAAG CCAAGGACCT TGCCCCTAAA 
AAGAAATATT TCTGTGAACT GTGCCTTGAT GATACCCTCT TTGCTCGTAC AACCAGCAAG 
ACCAAAGCAG ATAATATTTT CTGGGGCGAA CATTTTGAAT TCTACAGCCT TCCACCTCTT 
CATAGCATCA CAGTTCACAT TTATAAAGAT GTGGAAAAAA AGAAAAAAAA AGACAAGAAT 
AATTACGTCG GCCTAGTCAA CATTCCCACT GCCAGTGTGA CTGGTCGCCA GTTTGTAGAA 
AAGTGGTACC CAGTGAGTAC CCCTACGCCC AACAAAGGAA AGACAGGCGG ACCTTCTATT 
CGGATTAAAT CACGGTTCCA AACTATCACC ATTCTGCCTA TGGAGCAATA CAAAGAATTT 
GCAGAATTTG TCACCAGCAA CTACACCATG CTGTGTTCTG TCCTTGAACC AGTAATTAGT 
GTGAGAAATA AAGAGGAGTT GGCTTGTGCC TTAGTGCACA TTCTTCAAAG TACCGGCAGA 
GCCAAGGATT TTCTGACCGA CTTGGTGATG TCTGAGGTGG ATCGCTGTGG AGAGCATGAT 
GTCTTGATCT TCAGAGAGAA CACCATTGCC ACCAAATCCA TTGAGGAATA CCTCAAGTTG 
GTGGGACAAC AGTATCTTCA TGACGCATTG GGGGAGTTCA TCAAAGCTCT GTACGAGTCA 
GATGAGAACT GTGAGGTGGA GCCCAGCAGG TGTTCCTCTA GTGAACTGAT AGAGCATCAG 
AGCAACCTGA AAATGTGCTG TGAGCTGGCT TTCTGCAAGA TCATCAACTC GTACTGTGTT 
TTCCCTCGTG AGTTGAAAGA AGTGTTTGCG TCATGGAAGC AGCAGTGCCT GAACCGTGGC 
AAGCAGGACA TCAGCGAGCG GCTCATCAGT GCCTCGTTAT TTCTGCGTTT TCTGTGCCCA 
GCCATTATGT CTCCCAGTCT TTTCAACCTG ATGCAGGAGT ATCCTGATGA CCGCACATCT 
CGGACTCTGA CTCTAATTGC CAAGGTCATT CAGAACCTGG CTAACTTTGC CAAATTTGGT 
AACAAAGAAG AATATATGGC ATTCATGAAT GATTTTTTAG AACATGAATG GGGTGGAATG 
AAGCGCTTTC TTTTGGAGAT CTCTAATCCG GACACCATCT CGAACACACC AGGCTTTGAT 
GGTTACATTG ATCTGGGCCG AGAGCTTTCA GTTTTGCATT CCTTACTGTG GGAAGTAGTT 
TCCCAACTTG ATAAGGGTGA AAATTCCTTC CTACAGGCGA CCGTGGCAAA ATTGGGACCT 
CTCCCTCGAG TTCTTGCTGA TATTACCAAG TCTCTGACTA ATCCTACACC AATACAACAG 
CAACTAAGAC GCTTCACTGA GCATAACTCC AGTCCAAATG TCAGTGGAAG CCTCTCCTCT 
GGGCTGCAGA AAATATTCGA AGACCCCACT GACAGTGATT TGCATAAACT AAAATCTCCA 
AGCCAGGACA ACACTGATAG CTACTTCAGA GGGAAAACAT TACTGCTGGT GCAGCAAGCG 
TCCTCCCAGA GCATGACTTA CTCTGAAAAG GATGAGAAGG AAAGTAGCCT TCCTAACGGT 
CGGAGCATCT CCCTCATGGA CCTTCAGGAC ACTCACGCTG CTCAAGTGGA GCATGCACCT 
GTCATGCTCG ATGTGCCTAT GCGCTTAACT GGAAGCCAGC TTTCCATAAC CCAGGTGGCC 
AGCATCAAAC AACTGCGGGA AACCCAGAGC ACTCCCCAGA GCGCACCCCA AGTGAGAAGG 
CCCCTGCACC CGGCCTTGAA CCAGCCAGGC AGCCTCCAGC CCTTGTCATT CCAGAACCCT 
GTCTATCACC TCAATAACCC AATTCCGGCA ATGCCAAAGG CCTCTGTGGA TTCCAGTTTG 
GAGAACCTAA GCACTGCCAG TTCCAGAAGC CAAAGTAACA GTGAGGATTT CAAACTCAGC 
GGGCCCAGCA ATAGCAGTAT GGAAGATTTC ACCAAACGCA GCACCCAGAG CGAGGATTTC 
TCCAGGCGGC ATACTGTGCC AGACAGACAC ATACCTCTTG CTCTGCCACG GCAGAATAGT 
ACCGGGCAGG CCCAGATCCG GAAAATGGAC CAGGCTGGGT TAGGTGCCCG AGCCAAAGCC 
CCACCATCCC TGCCACATAG TGCTTCCTTA CGTAGCACGG GGAGCATGTC GGTGGCCTCT 
GCAGCCCTCA TGGCCGAACC TGTGCAGAAT GGGAGCCGGT CCCGGCAGCA GTCTTCCTCC 
TCCAGAGAGA GCCCTGTTCC CAAAGTGAGA GCGATCCAGA GACAACAAAC ACAGCAGGTT 
CAGTCACCTG TGGACTCTGC CACAATGTCC CCAGTAGAGA GGACAGCAGC CTGGGTTCTG 
AACAATGGAC AATATGAAGA GGATGTGGAA GAAACTGAGC AAAATCAAGA TGAATCCAAG 
CATGCTGAGA AGTACGAGCA GGAAATCACG AAGCTGAAGG AGCGCCTGCG AGTGTCCAGC 
CGGCGGCTGN GGGGGGGNNN NNTGGTGCGG GAGCGGCAAA TGCAGAAGCT GCTGCTGGAG 
TACAAGGCGC GGCTGGAGGA CAGCGAGGAG CGCCTGCGCA GGCAGCAGGA GGAGAAGGAC 
AGCCAGATGA AAAGCATCAT CAGCAGGCTC ATGGCTGTGG AAGAGGAACT GAAGAAAGAC 
CACGCTGAGA TGCAAGCCAT TATTGATGCA AAGCAAAAAA TAATTGATGC ACAGGAAAAA 
CGGATCGTGT CCCTGGATTC GGCCAACACC AGACTGATGA GCGCACTGAC CCAAGTGAAG 
GAGCGATACA GTGTGCAGGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC 
ATCACGGAGA ATGGTGAATT CAAAAACAGC AGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014938798.1
CDS	38..3736
Translation	MLSLRGESTNEPVREDVKGPPTHRLSCGQSPYTETTTWERRYCILTDSQLVLLNKEKEIPVEGGQEQQTDSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTSTSEKPNSMDTVNTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRARGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVEPSRCSSSELIEHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKQQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQDTHAAQVEHAPVMLDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLNNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTVPDRHIPLALPRQNSTGQAQIRKMDQAGLGARAKAPPSLPHSASLRSTGSMSVASAALMAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDESKHAEKYEQEITKLKERLRVSSRRLnullGnullnullnullVRERQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAIIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSVQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_015083312.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus RAS protein activator like 2 (RASAL2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015083312.1

ORF Insert Sequence:

ATGCTGAGTT TAAGAGGAGA ATCAACCAAT GAGCCAGTAA GAGAAGATGT GAAGGGGCCA 
CCCACGCATC GTCTGTCTTG CGGCCAGTCA CCCTACACGG AAACGACAAC GTGGGAGCGG 
AGGTACTGCA TCCTCACGGA CAGCCAGCTG GTGCTGCTCA ACAAGGAGAA GGAGATACCT 
GTGGAGGGAG GACAGGAGCA GCAGACAGAT TCCACCAAAG GGCGATGCCT GAGGAGAACC 
GTCAGTGTCC CTTCCGAGGG CCAGTTTCCT GAGTACCCAC CAGAGGGCGC CACTAAACTG 
GAGGTGCCGG CAGAAAGGTC CCCCCGCAGA CGGAGTATCT CAGGGACCAG TACATCAGAG 
AAACCCAACT CCATGGACAC TGTAAATACC TCACCCTTCA AAGTACCAGG GTTCTTCAGC 
AAGCGCCTGA AAGGCTCCAT CAAGAGGACC AAAAGCCAAT CAAAGCTTGA CAGAAACACG 
AGCTTTCGGC TTCCATCCCT TCGTAACACA GATGACAGGG CTCGTGGTCT GCCTAAACTA 
AAAGAGTCAC GTTCCCATGA GTCCTTGCTG AGCCCTTGCA GTGCAGTAGA GTGTCTGGAT 
CTTGGCAGAG GAGAGCCCGT GTCAGTGAAA CCACTCCATA GTAGCATCCT TGGACAAGAT 
TTCTGCTTTG AGGTTACCTA CTTAAGTGGA AGTAAATGCT TCAGCTGTAA CTCCGCTTCA 
GAGAGAGATA AGTGGATGGA AAACCTTCGT AGGACAGTTC AACCAAATAA GGACAATTGC 
AGACGAGCTG AAAATGTTCT TCGTTTATGG ATCATTGAAG CCAAGGACCT TGCCCCTAAA 
AAGAAATATT TCTGTGAACT GTGCCTTGAT GATACCCTCT TTGCTCGTAC AACCAGCAAG 
ACCAAAGCAG ATAATATTTT CTGGGGCGAA CATTTTGAAT TCTACAGCCT TCCACCTCTT 
CATAGCATCA CAGTTCACAT TTATAAAGAT GTGGAAAAAA AGAAAAAAAA AGACAAGAAT 
AATTACGTCG GCCTAGTCAA CATTCCCACT GCCAGTGTGA CTGGTCGCCA GTTTGTAGAA 
AAGTGGTACC CAGTGAGTAC CCCTACGCCC AACAAAGGAA AGACAGGCGG ACCTTCTATT 
CGGATTAAAT CACGGTTCCA AACTATCACC ATTCTGCCTA TGGAGCAATA CAAAGAATTT 
GCAGAATTTG TCACCAGCAA CTACACCATG CTGTGTTCTG TCCTTGAACC AGTAATTAGT 
GTGAGAAATA AAGAGGAGTT GGCTTGTGCC TTAGTGCACA TTCTTCAAAG TACCGGCAGA 
GCCAAGGATT TTCTGACCGA CTTGGTGATG TCTGAGGTGG ATCGCTGTGG AGAGCATGAT 
GTCTTGATCT TCAGAGAGAA CACCATTGCC ACCAAATCCA TTGAGGAATA CCTCAAGTTG 
GTGGGACAAC AGTATCTTCA TGACGCATTG GGGGAGTTCA TCAAAGCTCT GTACGAGTCA 
GATGAGAACT GTGAGGTGGA GCCCAGCAGG TGTTCCTCTA GTGAACTGAT AGAGCATCAG 
AGCAACCTGA AAATGTGCTG TGAGCTGGCT TTCTGCAAGA TCATCAACTC GTACTGTGTT 
TTCCCTCGTG AGTTGAAAGA AGTGTTTGCG TCATGGAAGC AGCAGTGCCT GAACCGTGGC 
AAGCAGGACA TCAGCGAGCG GCTCATCAGT GCCTCGTTAT TTCTGCGTTT TCTGTGCCCA 
GCCATTATGT CTCCCAGTCT TTTCAACCTG ATGCAGGAGT ATCCTGATGA CCGCACATCT 
CGGACTCTGA CTCTAATTGC CAAGGTCATT CAGAACCTGG CTAACTTTGC CAAATTTGGT 
AACAAAGAAG AATATATGGC ATTCATGAAT GATTTTTTAG AACATGAATG GGGTGGAATG 
AAGCGCTTTC TTTTGGAGAT CTCTAATCCG GACACCATCT CGAACACACC AGGCTTTGAT 
GGTTACATTG ATCTGGGCCG AGAGCTTTCA GTTTTGCATT CCTTACTGTG GGAAGTAGTT 
TCCCAACTTG ATAAGGGTGA AAATTCCTTC CTACAGGCGA CCGTGGCAAA ATTGGGACCT 
CTCCCTCGAG TTCTTGCTGA TATTACCAAG TCTCTGACTA ATCCTACACC AATACAACAG 
CAACTAAGAC GCTTCACTGA GCATAACTCC AGTCCAAATG TCAGTGGAAG CCTCTCCTCT 
GGGCTGCAGA AAATATTCGA AGACCCCACT GACAGTGATT TGCATAAACT AAAATCTCCA 
AGCCAGGACA ACACTGATAG CTACTTCAGA GGGAAAACAT TACTGCTGGT GCAGCAAGCG 
TCCTCCCAGA GCATGACTTA CTCTGAAAAG GATGAGAAGG AAAGTAGCCT TCCTAACGGT 
CGGAGCATCT CCCTCATGGA CCTTCAGGAC ACTCACGCTG CTCAAGTGGA GCATGCACCT 
GTCATGCTCG ATGTGCCTAT GCGCTTAACT GGAAGCCAGC TTTCCATAAC CCAGGTGGCC 
AGCATCAAAC AACTGCGGGA AACCCAGAGC ACTCCCCAGA GCGCACCCCA AGTGAGAAGG 
CCCCTGCACC CGGCCTTGAA CCAGCCAGGC AGCCTCCAGC CCTTGTCATT CCAGAACCCT 
GTCTATCACC TCAATAACCC AATTCCGGCA ATGCCAAAGG CCTCTGTGGA TTCCAGTTTG 
GAGAACCTAA GCACTGCCAG TTCCAGAAGC CAAAGTAACA GTGAGGATTT CAAACTCAGC 
GGGCCCAGCA ATAGCAGTAT GGAAGATTTC ACCAAACGCA GCACCCAGAG CGAGGATTTC 
TCCAGGCGGC ATACTGTGCC AGACAGACAC ATACCTCTTG CTCTGCCACG GCAGAATAGT 
ACCGGGCAGG CCCAGATCCG GAAAATGGAC CAGGCTGGGT TAGGTGCCCG AGCCAAAGCC 
CCACCATCCC TGCCACATAG TGCTTCCTTA CGTAGCACGG GGAGCATGTC GGTGGCCTCT 
GCAGCCCTCA TGGCCGAACC TGTGCAGAAT GGGAGCCGGT CCCGGCAGCA GTCTTCCTCC 
TCCAGAGAGA GCCCTGTTCC CAAAGTGAGA GCGATCCAGA GACAACAAAC ACAGCAGGTT 
CAGTCACCTG TGGACTCTGC CACAATGTCC CCAGTAGAGA GGACAGCAGC CTGGGTTCTG 
AACAATGGAC AATATGAAGA GGATGTGGAA GAAACTGAGC AAAATCAAGA TGAATCCAAG 
CATGCTGAGA AGTACGAGCA GGAAATCACG AAGCTGAAGG AGCGCCTGCG AGTGTCCAGC 
CGGCGGCTGN GGGGGGGNNN NNTGGTGCGG GAGCGGCAAA TGCAGAAGCT GCTGCTGGAG 
TACAAGGCGC GGCTGGAGGA CAGCGAGGAG CGCCTGCGCA GGCAGCAGGA GGAGAAGGAC 
AGCCAGATGA AAAGCATCAT CAGCAGGCTC ATGGCTGTGG AAGAGGAACT GAAGAAAGAC 
CACGCTGAGA TGCAAGCCAT TATTGATGCA AAGCAAAAAA TAATTGATGC ACAGGAAAAA 
CGGATCGTGT CCCTGGATTC GGCCAACACC AGACTGATGA GCGCACTGAC CCAAGTGAAG 
GAGCGATACA GTGTGCAGGT CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC 
ATCACGGAGA ATGGTGAATT CAAAAACAGC AGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RASAL2
Entrez Gene ID	106985106
Full Name	RAS protein activator like 2
Gene Type	protein-coding
Organism	Acinonyx jubatus(cheetah)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RASAL2 cDNA ORF clone, Acinonyx jubatus(cheetah)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

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